REPLIGEN KrosFlo TFF User manual

1 420-14871-001 Rev. 00
KrosFlo® TFF System - Auxiliary Scale
Model ACSS-20K
For use with:
•KrosFlo® KR2i Tangential Flow Filtration (TFF) System
•KrosFlo® KMPi Tangential Flow Filtration (TFF) System
User Guide

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
2 420-14871-001 Rev. 00
The information contained in this document is subject to change without notice.
Repligen Corporation makes no warranty of any kind with regards to this material, including, but not
limited to, the implied warranties of merchantability and fitness for a particular purpose.
Repligen Corporation shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language
without the prior written consent of Repligen Corporation.
Products are not intended for diagnostic or therapeutic use or for use in vivo with humans or
animals.
For further information, please contact Repligen Corporation at www.repligen.com.
©2020 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property
of Repligen Corporation and/or its affiliate(s) or their respective owners.
Customer Support
customerserviceU[email protected]
310-885-4600
Repligen Corporation
18617 South Broadwick Street
Rancho Dominguez, California 90220
www.repligen.com

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
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Contents
2.1 System assembly.....................................................................................................................8
2.2 Operating instructions..........................................................................................................10
2.2.1 Operational panel .......................................................................................................10
2.2.2 Operating the auxiliary scale ......................................................................................11
3.1 Good weighing practice........................................................................................................12
3.2 Zeroing..................................................................................................................................12
3.3 Taring....................................................................................................................................12
3.3.1 Entering tare value manually......................................................................................13
3.4 Units......................................................................................................................................13
3.4.1 Start unit .....................................................................................................................13
3.4.2 Temporary unit ...........................................................................................................13
4.1 External calibration...............................................................................................................14
4.2 User calibration ....................................................................................................................14
4.3 Calibration report .................................................................................................................14
5.1 Filter......................................................................................................................................14
5.2 Value release ........................................................................................................................15
5.3 Ambient conditions ..............................................................................................................15
5.4 Autozero function.................................................................................................................15
5.5 Tare entering mode..............................................................................................................15
5.6 Tare value memory...............................................................................................................15
5.6.1 Entering tare value to scale memory..........................................................................15
5.6.2 Selecting tare value from the scale memory..............................................................16
5.7 Last digit................................................................................................................................16
6.1 RS232 (1) port settings .........................................................................................................16
6.2 USB (A) port..........................................................................................................................16
6.3 USB (B) port ..........................................................................................................................17
7.1 Computer..............................................................................................................................17
7.1.1 Port .............................................................................................................................17
7.1.2 Continuous transmission ............................................................................................17
7.1.3 Printout interval for continuous transmission............................................................17
7.1.4 Printer .........................................................................................................................17
7.1.5 Port .............................................................................................................................18
7.2 Additional display .................................................................................................................18
7.2.1 Port .............................................................................................................................18
8.1 Calibration report .................................................................................................................18
8.2 GLP printout..........................................................................................................................19
9.1 Backlight ...............................................................................................................................19
9.2 ‘Beep’ sound.........................................................................................................................20
9.3 Automatic shutdown............................................................................................................20
9.4 Date and time .......................................................................................................................20
9.5 Default user settings.............................................................................................................20
11.1 Running working mode.........................................................................................................21

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
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11.2 Working mode local settings ................................................................................................21
11.2.1 Working mode accessibility ........................................................................................22
11.2.2 Save mode...................................................................................................................22
11.2.3 Automatic printout interval........................................................................................22
11.2.4 Lo threshold................................................................................................................23
12.1 Local settings ........................................................................................................................23
13.1 Local settings ........................................................................................................................23
13.1.1 Working mode selection.............................................................................................24
13.2 Setting reference sample mass by entering mass of a single part.......................................24
13.3 Setting reference sample mass by determining mass of a single part.................................24
14.1 Local settings ........................................................................................................................25
14.2 Declaring check weighing thresholds ...................................................................................25
15.1 Local settings ........................................................................................................................25
15.1.1 Working mode selection.............................................................................................26
15.2 Reference mass determined by weighing ............................................................................26
15.3 Reference sample mass by determining mass of a single part ............................................26
16.1 Local settings ........................................................................................................................26
16.2 Peak Hold operation.............................................................................................................27
17.1 Local settings ........................................................................................................................27
17.2 Totalizing operation..............................................................................................................27
18.1 Local settings ........................................................................................................................28
18.2 Animal Weighing operation..................................................................................................28
19.1 Cleaning ABS components....................................................................................................29
19.2 Cleaning stainless steel components....................................................................................29
19.3 Cleaning aluminum components..........................................................................................29
20.1 Dimensions ...........................................................................................................................30
20.2 Connectors arrangement......................................................................................................30

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
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List of tables
Table 1. KrosFlo® TFF System –Auxiliary Scale parts.........................................................................7
Table 2. Auxiliary scale keys .............................................................................................................10
Table 3. Auxiliary scale menu...........................................................................................................11
Table 4. Auxiliary scale menu navigation .........................................................................................11
Table 5. Good weighing practices ....................................................................................................12
Table 6. Calibration report variable list............................................................................................18
Table 7. GLP weighing printout variable list.....................................................................................19
Table 8. GLP date and time printout variable list.............................................................................20
Table 9. Scale Data ...........................................................................................................................21
Table 10. Working mode settings ......................................................................................................22
Table 11. Save mode options .............................................................................................................22
Table 12. Weighing –Local settings...................................................................................................23
Table 13. Parts Counting –Local settings...........................................................................................23
Table 14. +/- control –Local settings .................................................................................................25
Table 15. Percent Weighing –Local settings......................................................................................26
Table 16. Peak Hold –Local settings ..................................................................................................27
Table 17. Totalizing –Local settings...................................................................................................27
Table 18. Animal Weighing –Local settings.......................................................................................28
Table 19. Troubleshooting suggestions..............................................................................................31
Table 20. Error messages ...................................................................................................................31
List of figures
Figure 1. KrosFlo® TFF System - Auxiliary Scale components.............................................................7
Figure 2. Auxiliary scale parts.............................................................................................................7
Figure 3. Grounding spring check.......................................................................................................8
Figure 4. Rubber feet installation.......................................................................................................8
Figure 5. Weighing pan installation....................................................................................................9
Figure 6. Leveling the scale ................................................................................................................9
Figure 7. Interface cable to scale connection.....................................................................................9
Figure 8. Interface cable to octopus cable .......................................................................................10
Figure 9. Auxiliary scale keypad .......................................................................................................10
Figure 10. Calibration report example ...............................................................................................19
Figure 11. GLP printout example........................................................................................................19
Figure 12. Totalizing printout example ..............................................................................................28
Figure 13. Scale dimensions ...............................................................................................................30
Figure 14. Scale connectors................................................................................................................30
Figure 15. RS-232 connector ..............................................................................................................30

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
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Abbreviations
Acc Access
CAL Calibration
CdF Calibration difference
Conn Communication
CtP Calibration type
dSh Dashed
dAt Date
ducE Peripheral devices
Err Error
Func Function
InFo Information
Int Interval
Lo Low
Othr Other
Prnt Printouts
rEAd Readout
SiG Signature
tin Time

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
7 repligen.com 420-14871-001 Rev. 00
KrosFlo® TFF System –Auxiliary Scale
The KrosFlo ® TFF System –Auxiliary Scale assembly and operation may vary based on application.
The instructions below are for general use. For questions about specific application set up or
customization, please contact your local Repligen representative.
Figure 1. KrosFlo® TFF System –Auxiliary Scale components
1. Display and operations keypad
2. Weighing pan
1. General precautions
•Remove the contents from the packaging. If a part is missing, contact Repligen for
replacement
•Prior to the first use, carefully read this User Guide. Use the device only as intended
•Scales to be decommissioned must be decommissioned in accordance with valid legal
regulations
2. KrosFlo® TFF System –Auxiliary Scale
Table 1. KrosFlo® TFF System –Auxiliary Scale parts
Part description
Quantity
ACSS-20K scale
1
Weighing pan
1
Solid rubber foot
3
Rubber foot with spring
1
3’ Interface cable
1
Figure 2. Auxiliary scale parts
1. ACSS-20K scale400-15001-000 Rev.
2. Weighing pan
3. Rubber foot with spring
4. Solid rubber foot
5. 3’ Interface cable
1
2
3
4
5
1
2

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
8 repligen.com 420-14871-001 Rev. 00
1. Place the balance on a solid surface to ensure stability. Operate the balance in a room where
the temperature ranges between 10 - 40o C (50 - 104o F).
2. The balance must be positioned away from heat sources, magnetic fields, vibrations, and air
drafts to receive stable and repeatable results. Balance is to not be used for explosive
materials.
2.1 System assembly
1. Check the grounding spring located in the rubber foot with spring. The spring must jut
slightly out of the hole.
Figure 3. Grounding spring check
2. Install the rubber foot with spring into any freely selected opening. Install 3 x solid rubber
feet in remaining openings.
Figure 4. Rubber feet installation
3. Install the weighing pan.

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
9 repligen.com 420-14871-001 Rev. 00
Figure 5. Weighing pan installation
4. Level the scale by turning the feet and monitoring the leveling vial in the rear of the unit.
Continue to adjust the feet until the air bubble takes the central position.
Figure 6. Leveling the scale
5. Prior to switching the balance on, let it reach room temperature (estimated stabilization
time: 1 - 8 hours).
6. Connect the adapter cable (DB9-M) to the rear of the scale. During this process there should
be no load on the weighing pan.
Figure 7. Interface cable to scale connection
7. Connect the adapter cable (DB9-F) to the auxiliary component octopus cable.

KrosFlo® TFF System –Auxiliary Scale ACSS-20K
User Guide
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Figure 8. Interface cable to octopus cable
2.2 Operating instructions
2.2.1 Operational panel
Figure 9. Auxiliary scale keypad
Table 2. Auxiliary scale keys
Key
Function
Press to switch the weighing device on/off –hold the key for about 1 second
Function key, press to change the working mode
Press to send the weighing result to a printer or a computer
Press to zero the scale
Press to tare the scale