REPLIGEN KrosFlo ACSS-60K User manual

1
420-14871-002 Rev. 00
KrosFlo® TFF System - Auxiliary Scale
Model ACSS-60K
User Guide
For use with:
•KrosFlo® KR2i Tangential Flow Filtration (TFF) System
•KrosFlo® KMPi Tangential Flow Filtration (TFF) System
User Guide

KrosFlo® TFF System – Auxiliary Scale ACSS-60K
User Guide
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The information contained in this document is subject to change without notice.
Repligen Corporation makes no warranty of any kind with regards to this material, including, but not
limited to, the implied warranties of merchantability and fitness for a particular purpose.
Repligen Corporation shall not be liable for errors contained herein or for incidental or
consequential damages in connection with the furnishing, performance, or use of this material.
No part of this document may be photocopied, reproduced, or translated to another language
without the prior written consent of Repligen Corporation.
Products are not intended for diagnostic or therapeutic use or for use in vivo with humans or
animals.
For further information, please contact Repligen Corporation at www.repligen.com.
©2020 Repligen Corporation. All rights reserved. The trademarks mentioned herein are the property
of Repligen Corporation and/or its affiliate(s) or their respective owners.
Customer Support
customerserv[email protected]om
310-885-4600
Repligen Corporation
18617 South Broadwick Street
Rancho Dominguez, California 90220
www.repligen.com

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Contents
2.1 System assembly.....................................................................................................................8
2.2 Operating instructions..........................................................................................................10
2.2.1 Operational panel .......................................................................................................10
2.2.2 Operating the auxiliary scale ......................................................................................10
3.1 Good weighing practice........................................................................................................12
3.2 Zeroing..................................................................................................................................12
3.3 Taring ....................................................................................................................................12
3.3.1 Entering tare value manually......................................................................................13
3.4 Units......................................................................................................................................13
3.4.1 Start unit .....................................................................................................................13
3.4.2 Temporary unit ...........................................................................................................13
4.1 External calibration...............................................................................................................14
4.2 User calibration ....................................................................................................................14
4.3 Calibration report .................................................................................................................14
5.1 Filter......................................................................................................................................14
5.2 Value release ........................................................................................................................15
5.3 Ambient conditions ..............................................................................................................15
5.4 Autozero function.................................................................................................................15
5.5 Tare entering mode ..............................................................................................................15
5.6 Tare value memory...............................................................................................................16
5.6.1 Entering tare value to scale memory..........................................................................16
5.6.2 Selecting tare value from the scale memory..............................................................16
5.7 Last digit................................................................................................................................16
6.1 RS232 (1) port settings .........................................................................................................17
7.1 Computer..............................................................................................................................17
7.1.1 Port .............................................................................................................................17
7.1.2 Continuous transmission ............................................................................................17
7.1.3 Printout interval for continuous transmission............................................................18
7.1.4 Printer .........................................................................................................................18
7.1.5 Port .............................................................................................................................18
7.2 Additional display .................................................................................................................18
7.2.1 Port .............................................................................................................................18
8.1 Calibration report .................................................................................................................18
8.2 GLP printout..........................................................................................................................19
9.1 Backlight ...............................................................................................................................20
9.2 ‘Beep’ sound .........................................................................................................................20
9.3 Automatic shutdown ............................................................................................................20
9.4 Date and time .......................................................................................................................20
9.5 Default user settings.............................................................................................................21
11.1 Running working mode.........................................................................................................22
11.2 Working mode local settings ................................................................................................22
11.2.1 Working mode accessibility ........................................................................................22

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11.2.2 Save mode...................................................................................................................22
11.2.3 Automatic printout interval........................................................................................23
11.2.4 Lo threshold ................................................................................................................23
12.1 Local settings ........................................................................................................................23
13.1 Local settings ........................................................................................................................24
13.1.1 Working mode selection.............................................................................................24
13.2 Setting reference sample mass by entering mass of a single part .......................................24
13.3 Setting reference sample mass by determining mass of a single part.................................24
14.1 Local settings ........................................................................................................................25
14.2 Declaring checkweighing thresholds ....................................................................................25
15.1 Local settings ........................................................................................................................26
15.1.1 Working mode selection.............................................................................................26
15.2 Reference mass determined by weighing ............................................................................26
15.3 Reference sample mass by determining mass of a single part ............................................26
16.1 Local settings ........................................................................................................................27
16.2 Peak Hold operation .............................................................................................................27
17.1 Local settings ........................................................................................................................27
17.2 Totalizing operation..............................................................................................................28
18.1 Local settings ........................................................................................................................29
18.2 Animal Weighing operation..................................................................................................29
19.1 Cleaning ABS components....................................................................................................29
19.2 Cleaning stainless steel components....................................................................................30
19.3 Cleaning aluminum components..........................................................................................30
20.1 Dimensions ...........................................................................................................................31
20.2 Connectors arrangement......................................................................................................31

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List of tables
Table 1. Auxiliary scale parts ..............................................................................................................7
Table 2. Auxiliary scale keys .............................................................................................................10
Table 3. Auxiliary scale menu ...........................................................................................................10
Table 4. Auxiliary scale menu navigation .........................................................................................11
Table 5. Good weighing practices ....................................................................................................12
Table 6. Calibration report variable list............................................................................................19
Table 7. GLP weighing printout variable list.....................................................................................19
Table 8. GLP date and time printout variable list.............................................................................21
Table 9. Scale Data ...........................................................................................................................21
Table 10. Working mode settings ......................................................................................................22
Table 11. Save mode options .............................................................................................................23
Table 12. Weighing – Local settings ...................................................................................................23
Table 13. Parts Counting – Local settings...........................................................................................24
Table 14. +/- control – Local settings .................................................................................................25
Table 15. Percent Weighing – Local settings......................................................................................26
Table 16. Peak Hold – Local settings ..................................................................................................27
Table 17. Totalizing – Local settings...................................................................................................28
Table 18. Animal Weighing – Local settings.......................................................................................29
Table 19. Troubleshooting suggestions..............................................................................................32
Table 20. Error messages ...................................................................................................................32
List of figures
Figure 1. KrosFlo® TFF - Auxiliary Scale components .........................................................................7
Figure 2. Auxiliary scale parts.............................................................................................................7
Figure 3. Packaging removal...............................................................................................................8
Figure 4. Transport lock removal........................................................................................................8
Figure 5. Leveling the scale ................................................................................................................8
Figure 6. Replace weighing pan
..........................................................................................................9
Figure 7. Interface cable to scale connection.....................................................................................9
Figure 8. Interface cable to octopus cable .........................................................................................9
Figure 9. Auxiliary scale keypad .......................................................................................................10
Figure 10. Calibration report example ...............................................................................................19
Figure 11. GLP printout example........................................................................................................20
Figure 12. Totalizing printout example ..............................................................................................28
Figure 13. Scale dimensions ...............................................................................................................31
Figure 14. Scale connectors................................................................................................................31
Figure 15. RS-232 connector ..............................................................................................................31

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Abbreviations
Acc Access
CAL Calibration
CdF Calibration difference
Conn Communication
CtP Calibration type
dSh Dashed
dAt Date
ducE Peripheral devices
Err Error
Func Function
InFo Information
Int Interval
Lo Low
Othr Other
Prnt Printouts
rEAd Readout
SiG Signature
tin Time

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KrosFlo® TFF System – Auxiliary Scale
The KrosFlo® TFF System – Auxiliary Scale assembly and operation may vary based on application.
The instructions below are for general use. For questions about specific application set up or
customization, please contact your local Repligen representative.
Figure 1. KrosFlo® TFF - Auxiliary Scale components
1. Display and operations keypad
2. Weighing pan
1. General precautions
•Remove the contents from the packaging. If a part is missing, contact Repligen for
replacement
•Prior to the first use, carefully read this User Guide. Use the device only as intended
•Scales to be decommissioned must be decommissioned in accordance with valid legal
regulations
2. KrosFlo® TFF System - Auxiliary Scale
Table 1. Auxiliary scale parts
Part description Quantity
ACSS-60K scale
1
6’ Interface cable
1
Figure 2. Auxiliary scale parts
1. ACSS-60K scale
2. 6’ Interface cable
1. Place the balance on a solid surface to ensure stability. Operate the balance in a room where
the temperature ranges between 10 - 40o C (50 - 104o F).
2. The balance must be positioned away from heat sources, magnetic fields, vibrations, and air
drafts to receive stable and repeatable results. Balance is to not to be used for explosive
materials.
1
2
1
2

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2.1 System assembly
1. Remove unit from packaging.
Figure 3. Packaging removal
2. Remove unit transport locks. Once removed replace the weighing pan.
Figure 4. Transport lock removal
1. Level the scale by turning the feet and monitoring the leveling vial. Continue to adjust the
feet until the air bubble takes the central position.
Figure 5. Leveling the scale
2. Replace weighing pan.

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Figure 6. Replace weighing pan
3. Prior to switching the balance on, let it reach room temperature (estimated stabilization
time: 1 - 8 hours).
4. Connect the adapter cable (DB9-M) to the rear of the scale. During this process there should
be no load on the weighing pan.
Figure 7. Interface cable to scale connection
5. Connect the adapter cable (DB9-F) to the auxiliary component octopus cable.
Figure 8. Interface cable to octopus cable

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2.2 Operating instructions
2.2.1 Operational panel
Figure 9. Auxiliary scale keypad
Table 2. Auxiliary scale keys
Key Function
Press to switch the weighing device on/off – hold the key for about 1 second
Function key, press to change the working mode
Press to send the weighing result to a printer or a computer
Press to zero the scale
Press to tare the scale
2.2.2 Operating the auxiliary scale
Table 3. Auxiliary scale menu
Function group number Function group name Description
P1
CAL
User calibration
P2
rEAd
Readout parameters
P2
Func
Working modes
P4
Conn
Communication
P5
ducE
Peripheral devices
P6
Prnt
Printouts
P7
Othr
Operation-related functions
P8
InFo
Scale information
P9
Unit
Units
In order to navigate the auxiliary scales menu, utilize the keypad.

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Table 4. Auxiliary scale menu navigation
Key Description
+ Press to enter the main menu
+
Press to:
•Enter tare manually
•Enter tare from tare database
•Change value by 1 digit up
•
Scroll the menu up
+ Press to view date/time
Press to:
•Scroll the menu down
•
Change current parameter value
Press to enter given submenu. Press to modify given parameter
Press to confirm modification
Press to:
•Exit, function remains unmodified
•Move one menu level up

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3. Weighing
3.1 Good weighing practice
To assure long-term operation and correct mass measurement, follow the rules presented below:
Table 5. Good weighing practices
3.2 Zeroing
1. To zero mass indication press key, during this process the weighing pan must be
empty.
2. With the zeroing of the scale, the pictograms and are displayed. The scale can only
be zeroed when the indication is stable.
3. Indication can be zeroed only within ±2% range of the maximum capacity. If the zeroed
value is greater than ±2% of the maximum capacity, then the scale displays message <Err2>,
and short sound signal is heard.
3.3 Taring
1. To determine the net weight value, load the weighing pan with a package. Wait for the
stable indication, , and press key.
2. The zero indication and the following pictograms are displayed: Net and .
3. The scale has now been tared. Upon loading, net mass is displayed.
Recommendation
Load the weighing pan steadily avoiding mechanical shocks
Place weighed loads centrally on the weighing pan (eccentricity
errors are specified by EN 45501 standard, point 3.6.2.)
Do not apply concentrated force (total load in one point)
Avoid side loading, in particular side shocks
NO
YES
YES
NO
NO
NO

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4. Taring can be carried out repeatedly within the whole weighing range. Do not exceed the
maximum capacity, i.e. sum of tare weight value and load weight value must be lower than
the maximum capacity value.
5. Upon unloading, the sum of tared masses with a minus sign is displayed. To return to zero
point press key.
3.3.1 Entering tare value manually
1. Press and keys combination, tare value edit box is displayed.
2. Enter tare value. To do so press to set digit value (0 - 9) and to select digit to be
edited.
3. Press key to confirm, the scale returns to the weighing mode, modified tare value with
‘-‘ sign is displayed.
4. You can enter tare value at any time during the weighing operation.
3.4 Units
<P9.Unit> parameter group enables change of start unit and temporary unit. The units can be
changed during weighing or during other working modes operation. ‘Parts Counting’ and ‘Percent
Weighing’ modes are exceptions.
3.4.1 Start unit
1. Enter <P9.Unit/9.1.UnSt> submenu.
2. Press key, available units are displayed successively one by one. Available units are:
kg (kilogram), g (gram), lb (pound), N (Newton).
3. Select start unit and press key. To return to home screen press key.
4. Upon restart, the scale runs with the set start unit.
3.4.2 Temporary unit
Temporary unit runs from the moment it is set to the scale shut-down and restart.
1. Enter <P9.Unit/9.2.Unin> submenu
2. Press key, available units are displayed successively one by one. Available units are:
kg (kilogram), g (gram), lb (pound), N (Newton).
3. Select start unit and press key. To return to home screen press key.
4. Calibration
In order to ensure the highest weighing accuracy, it is recommended to periodically introduce
corrective factor of indications to scale memory. This factor is a known reference weight which is
used to perform a calibration of the scale.
Calibration is recommended to be carried out:

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1. Prior to weighing.
2. If long breaks between successive measuring series occurs.
3. If the ambient temperature has changed dynamically.
4. If the scale has been relocated.
4.1 External calibration
External calibration must be carried out using an external calibration weight of class F2.
1. Enter <P1.CAL / 1.1.CA-E> submenu, the text <UnLoAd> will be displayed.
2. Remove any load from the weighing pan and press key.
3. Mass of the empty weighing pan is determined; this is signaled with display of dash <- >.
4. Text <LoAd> is displayed along with required mass value, <50.000kg>.
5. Load the required mass value, press key.
6. Weigh mass is determined; this is signaled with display of dash <- >. Once complete, text
<UnLoAd> will be displayed.
7. Remove mass from weighing pan, scale returns to <P1.CAL / 1.1.CA-E> submenu.
4.2 User calibration
User calibration must be carried out using an external calibration weight of class F2. The calibration
weight value is specified by sure and must be of a mass equal or greater than 30% of maximum
capacity.
1. Enter <P1.CAL / 1.2.CA-u> submenu, edit box for declaring weight mass is displayed (the
mass value must be ≥30% of the maximum capacity value).
2. Enter weight mass value and press key, the text <UnLoAd> will be displayed.
3. Remove any load from the weighing pan and press key.
4. Mass of the empty weighing pan is determined; this is signaled with display of dash <- >.
5. Text <LoAd> is displayed along with user inputted mass value, i.e. <20.000kg>.
6. Load the required mass value, press key.
7. Weigh mass is determined; this is signaled with display of dash <- >. Once complete, text
<UnLoAd> will be displayed.
8. Remove mass from weighing pan, scale returns to <P1.CAL / 1.2.CA-u> submenu.
4.3 Calibration report
The calibration report is automatically printed (using scale-connected printer) at the end of each
calibration process. To declare report content, go to <P6.1.CrEP> submenu.
5. Scale parameters
Scale parameters are set to adjust the weighing device to ambient conditions (filters) or individual
needs (autozero, on/off, tare values memory). These parameters can be found in <P2.rEAd>
submenu.
5.1 Filter
1. Enter <P2.rEAd / 2.1.Fil> submenu.

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2. Press key, filter values are displayed successively one by one, where: 1 - Fast, 2 -
Average, 3 – Slow.
3. Set respective value and press key to confirm, next go to the home screen.
Note:The higher filter value, the longer the indication takes to stabilize.
5.2 Value release
Enter this parameter to adjust rate of stabilization of the measurement result. Depending on the
selected option, weighing time is either shorter or longer.
1. Enter <P2.rEAd / 2.2.APPr> submenu.
2. Press key, filter values are displayed successively one by one, where: F_P – Fast and
Reliable, PrEc - Reliable, FASt – Fast.
3. Set respective value and press key to confirm, next go to the home screen.
5.3 Ambient conditions
4. Parameter relating to ambient and environmental conditions of the workstation. Enter this
parameter and set 'nStAb' value if the ambient conditions are unfavorable (air drafts,
vibrations).
5. Enter <P2.rEAd / 2.3.Enut> submenu.
6. Press key, filter values are displayed successively one by one, where: nStAb – unstable,
StAb – stable.
7. Set respective value and press key to confirm, next go to the home screen.
5.4 Autozero function
‘Autozero’ function has been designed to enable automatic control of zero indication. This
guarantees precise weighing results.
There are, however, some cases where this function can be a negative factor in the measuring
process, e.g. very slow loading of the weighing pan (load adding, e.g. pouring, filling). In such case, it
is recommended to disable the function.
1. Enter <P2.rEAd / 2.4.Aut> submenu.
2. Press key, filter values are displayed successively one by one, where: YES – Function
enabled, no – Function disabled.
3. Set respective value and press key to confirm, next go to the home screen.
5.5 Tare entering mode
The tare is entered using and keys combination. There are two enter modes.

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1. Enter <P2.rEAd / 2.6.ttr> submenu.
2. Press key, filter values are displayed successively one by one: tArEH -to enter tare
value manually, tArnn – to enter tare value that is stored in scale memory.
3. Set respective value by using and keys.
4. Press key to confirm, next go to the home screen.
5.6 Tare value memory
It is possible to store 10 tare values in scale memory.
5.6.1 Entering tare value to scale memory
1. Enter <P2.rEAd / 2.7.tArn> submenu, name of tare no. 1 from tare database is displayed
(<tArE 0>), to select a different record press key.
2. Select respective entry and press key, tare value edit box is displayed.
3. Enter tare value, to do this press to select digit to be edited and to set digit value
(0 - 9).
4. Press key to confirm, <tArE 0> is displayed.
5. Press to return to home screen.
5.6.2 Selecting tare value from the scale memory
1. Enter <P2.rEAd / 2.7.tArn> submenu, name of tare no. 1 from tare database is displayed
(<tArE 0>), to select a different record press key.
2. To select a tare value press key.
3. The set tare value is displayed on the screen with a minus sign, Net symbol is shown in the
upper-left corner of the screen.
Note:The tare value acquired from the weighing device memory is not remembered upon the
weighing device restart.
5.7 Last digit
Function designed to disable display of the last weighing indication digit, this results with less
accurate measurement.
1. Enter <P2.rEAd / 2.8.LdiG> submenu.
2. Press key, filter values are displayed successively one by one, where: ALAS – Last digit
always on, nEur – Last digit always off, and uuSt – Last digit on only when the weighing
indications is stable.

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3. Set respective value and press key to confirm, next go to the home screen.
6. Communication
Communication between the scale and peripheral devices is established via RS232 port. To set the
ports go to <P4.Conn> submenu.
6.1 RS232 (1) port settings
1. Enter <P4.Conn / 4.1.rS1> submenu.
2. Submenu <4.1.1.bAd> sets the baud rate. Press key, and rates are displayed
successively one by one: 2400, 4800, 9600, 19200, 38400, 57600, 115200 bit/s.
3. Submenu <4.1.1.bAd> sets the baud rate. Press key, and rates are displayed
successively one by one: 2400, 4800, 9600, 19200, 38400, 57600, 115200 bit/s.
4. Set respective value and press key to confirm, next go to the home screen.
7. Peripheral devices
<P5.ducE> menu contains list of devices connecting with the scale.
7.1 Computer
<5.1.PC> submenu allows to:
•Select port to which the computer is connected
•Enable/disable continuous transmission
•Set frequency of printouts for continuous transmission
7.1.1 Port
1. Enter <P5.1.PC / 5.1.1.Prt> submenu.
2. Press key, port options are displayed successively, where: nonE – none, rS1 – RS232.
3. Set respective value and press key to confirm, next go to the home screen.
7.1.2 Continuous transmission
1. Enter <P5.1.PC / 5.1.2.Cnt> submenu.
2. Press key, transmission options are displayed successively, where: nonE – Continuous
transmission disabled, CntA – Continuous transmission in basic unit, Cntb – Continuous
transmission in current/temporary unit.
3. Set respective value and press key to confirm, next go to the home screen.

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7.1.3 Printout interval for continuous transmission
Parameter to set frequency of printout for continuous transmission. Printout interval is set in
seconds, with 0.1 [s] accuracy within 0.1 [s] – 3600 [s] range.
1. Enter <P5.1.PC / 5.1.3.Int> submenu.
2. Enter transmission rate, to do this press to select digit to be edited and to set
digit value (0 - 9).
3. Set respective value and press key to confirm, next go to the home screen.
7.1.4 Printer
<5.2.Prtr> submenu allows to:
•Select port to which the printer is connected
7.1.5 Port
1. Enter <P5.2.Prtr / 5.2.1.Prt> submenu.
2. Press key, options are displayed successively, where: nonE – No port selected, rS1 –
RS232.
3. Set respective value and press key to confirm, next go to the home screen.
7.2 Additional display
<5.3.AdSP> submenu allows to:
•Select port to which the additional display is connected
7.2.1 Port
1. Enter <P5.3.AdSP / 5.3.1.Prt> submenu.
2. Press key, options are displayed successively, where: nonE – No port selected, rS1 –
RS232 (1).
3. Set respective value and press key to confirm, next go to the home screen.
8. Printouts
8.1 Calibration report
<P6.1.CrEP> is a group of parameters allowing to declare variables that are to be printed on the
calibration report printout. Each variable allows for accessibility attribute: YES – print, no – do not
print. Calibration report is automatically generated at the end of each calibration process.

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Table 6. Calibration report variable list
Submenu Name Description
6.1.1.
CtP
Calibration type
6.1.2.
dAt
Calibration date
6.1.3.
tin
Calibration time
6.1.4.
Idb
Scale serial number
6.1.5.
CdF
Difference between mass of a calibration weight measured during the last
calibration and mass of the currently measured calibration weight
6.1.6.
dSh
Dashed line separating printout data and signature fields
6.1.7.
SiG
An area for the signature of an operator carrying out the calibration
Figure 10. Calibration report example
8.2 GLP printout
<P6.2.GLP> is a group of parameters allowing to declare variables that are to be printed on a
weighing printout. Each variable allows for accessibility attribute: YES – print, no – do not print.
Table 7. GLP weighing printout variable list
Submenu Name Description
6.2.1. dAt
Weighing date
6.2.2. tin
Weighing time
6.2.3. n
Net weight value of performed weighing in a basic measuring unit
6.2.4. t
Tare weight value in a current unit
6.2.5. b
Gross weight value in a current unit
6.2.6. CrS
Current weighing result (net weight) in a current unit
6.2.7. CrP
The last adjustment report generated in accordance with settings declared for the
adjustment report printout

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Figure 11. GLP printout example
9. Miscellaneous parameters
<P7.Othr> is a group of parameters enabling to customize scale operation.
9.1 Backlight
Parameter allowing to change display brightness, the brightness can be changed within 0% - 100%
range.
1. Enter <P7.Othr / 7.1.bLbt> submenu.
2. Press key, options are displayed successively, where: nonE – Backlight off, 10 – Display
brightness low limit value in [%], and 100 – Display brightness high limit value in [%] .
3. Set respective value and press key to confirm, next go to the home screen.
9.2 ‘Beep’ sound
Parameter allowing to enable/disable sound signal informing the operator about pressing panel
key(s).
1. Enter <P7.Othr / 7.2.bEEP> submenu.
2. Press key, options are displayed successively, where: no – sound signal disabled, YES –
sound signal enabled.
3. Set respective value and press key to confirm, next go to the home screen.
9.3 Automatic shutdown
Parameter allowing to set time interval, in [min], after which the weighing device shuts down
automatically. If the indication is stable during the declared time interval, the device is shut down.
Shutdown function is inactive, and the device cannot be turned off if any process is started or if you
operate the menu.
1. Enter <P7.Othr / 7.3.t1> submenu.
2. Press key, options are displayed successively, where: nonE – Function disabled, 1, 2, 3,
5, 10.
3. Set respective value and press key to confirm, next go to the home screen.
9.4 Date and time
Parameter allowing to set current date and time and to specify date and time format.