REVCO Ultima II User manual

305978H02 Rev A
©2003
All rights reserved
Printed in U.S.A.
Ultima II and Elite II
Laboratory CO2Incubators
Installation and Operation Manual
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . 2
Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Key Switch and Control Operation . . . . . . . . . . . . . 8
Control Systems Theory . . . . . . . . . . . . . . . . . . . . 13
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . 19

Installation and Operation
Ultima II and Elite II Incubators
i
Table of Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Features and Benefits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
1.2 Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
2 Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
3 Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
4 Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.1.1 Included Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.2 Set-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.3 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.4 Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
4.5 Wiring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
5 Initial Start-Up Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
5.1 Start-Up Prompts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.2 Installation Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.3 Power and Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.4 Level and Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.5 Shelving, HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.5.1 Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.5.2 HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.6 Water Jacket Fill . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.7 Re-level Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.8 Set Up CO2 Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.9 Set Up O2 or N2 Supply (Tri-Gas Units Only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.10 Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
5.11 Setpoint Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Installation and Operation
Ultima II and Elite II Incubators
ii
6 Key Switch and Control Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
6.1 Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
6.2 Control Panel Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
6.3 Display Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
6.4 Setpoint and Alarm Programming Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
6.5 Service Mode Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
6.6 Restore Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
6.7 Alarm Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
6.8 Calibration (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.8.1 CO2Measurement and Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.8.2 Temperature Correlation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.9 Remote Alarm Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
7 Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
7.1 Temperature Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
7.2 Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
7.3 Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
8.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.2 CO2Filter Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.3 Draining the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.4 Decontamination Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
8.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
8.6 Door Seal Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
9 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
10 Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Installation and Operation
Ultima II and Elite II Incubators
1
1 Introduction
Figure 1. Ultima II and Elite II Incubators
1.1 Features and Benefits
Revco UltimaTM II and EliteTM II incubators are designed to
create a stable, reliable environment for cell culture applications.
They operate at temperatures ranging from 5°C above ambient
temperature to +40°C.
•Convenience Outlet. A receptacle rated at 115 VAC, 75 watts
maximum, is located at the rear of the incubator. The outlet is
ideal for electronic recorders or other small loads. Do not
exceed the electrical capacity of this outlet.
•Alarms:
–High and low level CO2and (on Ultima tri-gas models) O2
setpoint alarms, adjustable to within ±1% of setpoint.
–High and low temperature alarms settable by the user.
•Chamber Drain. All models are provided with a chamber
drain valve at the bottom left of the cabinet.
•CO2tank switchover and Alarm.
•IntrLogicTM II microprocessor control system with large,
easy-to-read display. On program request, software steps the
user through initial installation and startup procedures.
•Keyed power/alarm switch provides setpoint security.
•Units stackable.
•An in-chamber HEPA filter is standard for Ultima models,
optional for Elite models.
•The IncuSoftTM interface for RS485 data port
communications is standard for Ultima models, optional for
Elite models.
1.2 Requirements
•High quality two-stage, low pressure 30 psig pressure
regulators are required for proper operation of the CO2and
(if applicable) O2gas supplies, 15 psig at the incubator.
•CO2used in the incubator must be at least 99.9% pure.
•In-line gas supply filters must be used on the CO2supply and
(on tri-gas units) the O2supply to prevent damage to the
solenoid valve(s). One filter for each gas is supplied with the
startup kit:
–Type: Microbiological
–Specification: 0.3 micron
–Location: rear of unit
•Each chamber contains approximately 10 gallons of jacket
water when filled. The water takes six to eight hours to heat
from 20°to 37°C. This time must be allowed for the interior
temperature to stabilize before any adjustments or permanent
calibrations are made.
Welded Wall
Seamless Wall

Installation and Operation
Ultima II and Elite II Incubators
2
2 Safety Precautions
In this manual and on labels attached to this product, the words
WARNING and CAUTION mean the following:
•WARNING: a potentially hazardous situation which, if not
avoided, could result in serious injury or death.
•CAUTION: a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury or damage to
the equipment.
The following symbols are used in caution, warning and
informational labels attached to the incubator:
Before installing, using or maintaining this product, please be sure
to read this manual and product warning labels carefully. Failure to
follow these instructions may cause this product to malfunction,
which could result in injury or damage.
Below are important safety precautions that apply to this product:
•Use this product only in the way described in the product
literature and in this manual. Before using it, verify that this
product is suitable for its intended use.
•Do not modify system components, especially the controller.
Use OEM exact replacement equipment or parts. Before use,
confirm that the product has not been altered in any way.
•Disconnect the unit from all power sources before cleaning,
troubleshooting, or performing other maintenance on the
product or its controls. To disconnect power supply to the
incubator, unplug the supply cord at the back of the incubator.
Note that turning the key switch on the front control panel to
the Off position is not sufficient to disconnect power.
WARNING! The user is responsible for carrying out
appropriate decontamination procedures when
hazardous materials are spilled on or inside the
incubator.
3 Operating Standards
The incubators described in this manual are classified for use as
stationary equipment in a Pollution Degree 2 and Overvoltage
Category II environment, according to the UL61010A-1 and IEC
664 standards.
These units are designed to operate under the following
environmental conditions:
•Indoor use
•Altitude up to 2000m
•Maximum relative humidity 80% for temperatures up to 31ºC
•Main supply voltage fluctuations not to exceed 10% of the
nominal voltage.
4 Pre-Installation
4.1 Unpacking
At delivery, examine the exterior for physical damage while the
carrier’s representative is present. If exterior damage is present,
carefully unpack and inspect the unit and all accessories for
damage. If there is no exterior damage, unpack and inspect the
equipment within five days of delivery. If you find any damage,
keep the packing materials and immediately report the damage to
the carrier. Do not return goods to the manufacturer without
written authorization. When submitting a claim for shipping
damage, request that the carrier inspect the shipping container
and equipment.
4.1.1 Included Parts
The following items are packaged and shipped inside the
incubator cabinet:
•Shelves and shelf brackets
•3/16 in. (476 mm) ID clear tubing for the gas connection (a Y
fitting is also supplied with dual-chamber units)
•Decontamination kit: 0.3 micron gas filter, replacement
Blower Wheel, CO2Sensor gasket, and Injection Port
Assembly gasket
•Tubing, funnel, and bottle of water conditioning crystals for
filling the water jacket
•Humidity pan
•HEPA filter (if one comes with your unit)
•A thin, 1/2” open-end wrench to adjust leveling legs and
relocate the outer door hinge
•Stacking brackets (for dual models)
4.2 Set-up
Remove shelves from the inside of the incubator and clean the
incubator (refer to Section 8.1 on page 14). The shelves and
brackets can be autoclaved at this time.
4.3 Location
Install the unit in a level area free from vibration with a minimum
of three inches (7.6 cm) of space on the sides and rear and
12 inches (30.5 cm) at the top. The floor must be able to support
40 PSI (single chamber incubator) or 75 PSI (double chamber
incubator).
Be sure to position the incubator so that the power cord can
easily be reached to disconnect power. Do not position the
equipment in direct sunlight or near any HVAC duct/diffusers.
The ambient temperature range at the location must be 59 to
90°F (15 to 32°C).
caution, info
electrical hazard
hot surface
earth ground
protective conductor terminal
on
off
alternating
current

Installation and Operation
Ultima II and Elite II Incubators
3
4.4 Gas Supplies
Verify that the incubator gas supplies are available near the
installation area: CO2for all incubators, also O2 and N2for tri-
gas models. The required gas supply pressure is 15 PSI,
controlled by a high-quality, two-stage regulator suitable for each
connected input gas.
Do not connect the gas at this time.
4.5 Wiring
CAUTION! Connect the equipment to the correct
power source. Be sure to operate the incubator at the
voltage specified on the dataplate. Incorrect voltage
can result in severe damage to the equipment.
WARNING! For personal safety and trouble-free
operation, this unit must be properly grounded before
it is used. Failure to ground the equipment may cause
personal injury or damage to the equipment. Always
conform to the National Electrical Code and local
codes. Do not connect the unit to overloaded power
lines.
The incubators described in this manual are rated either for
supply voltage 115/120 VAC, single-phase, 60Hz, 6 amps, or for
supply voltage 230 VAC, single-phase, 50Hz, 3 amps. Be sure to
check your dataplate for correct information.
Always connect the unit to a dedicated (separate) circuit. Do not
exceed the electrical and temperature ratings printed on the
dataplate located near the upper hinge of the unit. Electrical
codes require fuse or circuit breaker protection for branch circuit
conductors. Use time delay fuses for #12 AWG circuits.

Installation and Operation
Ultima II and Elite II Incubators
4
5 Initial Start-Up Procedures
Figure 2. Control Panel: First Start-Up Prompt
IMPORTANT NOTE: When setting up your incubator for the
first time, be sure to follow the control instructions on the
sticker provided on the control panel, and in Section 5.3
below.
5.1 Start-Up Prompts
When you first start up your incubator, the control display will go
through several prompts that will guide you through the entire
set-up process, from leveling the incubator to adjusting the
operating parameters.
During normal operation, the control panel display looks like
Figure 8 on page 8. The next chapter explains all the control
functions available during normal operation. On initial start-up,
however, you will see a series of prompts similar to the one
shown above. This chapter explains the start-up prompts and the
procedures they ask you to follow.
5.2 Installation Tools
Tools you will need to complete installation are:
•A sharp knife or scissors suitable for cutting plastic tubing
•A bubble level, at least 9 in. (23 cm) long, for leveling the
incubator
•A thin, 1/2 in. (1.25 cm) open-end wrench (provided with
your incubator) to adjust the leveling feet
5.3 Power and Key Switch
Turn the key switch (shown in the figure above) to the “1”
position.
The Revco logo will then roll across the display.
While the logo is displayed, press and hold the Increase button
() until the logo disappears to initiate the special start-up
prompts. The first prompt is the one shown above: if you see a
temperature display instead, you haven’t pressed the Increase
button long enough — turn the key switch back to “0” and try
again.
5.4 Level and Position
The first prompt asks you to level the incubator. It is very
important to level the unit both left to right and front to back.
1. Place a bubble level across the top of the incubator (side to
side).
2. Use the thin, 1/2 in.(1.25 cm) open-end wrench to adjust the
leveling feet located at the base of the incubator.
3. Repeat steps 1 and 2, this time placing the bubble level on top
of the incubator front to back.
4. Slide the incubator into place.
After the water jacket is filled, the incubator must be re-leveled
left to right and front to back.
When the incubator is level, press to advance to the next
prompt.
Key Switch
Mode
Level incubator
- complete

Installation and Operation
Ultima II and Elite II Incubators
5
5.5 Shelving, HEPA Filter
The next prompt asks you to install the shelves and the HEPA
filter.
5.5.1 Shelves
The incubator shelves are fully adjustable. First install the side
support walls and top duct. Then determine the location you want
for each shelf and install the shelf brackets (refer to Figure 3).
1. Place the base pan with the hole for the humidity pan facing
the front of the cabinet.
2. Place a shelf support wall on top of the base on the left side,
with the indicator hole toward the front bottom of the unit.
The curve of the support wall aligns with the curve of the
chamber.
3. Install the top air duct, making sure that the circular hole is to
the right directly under the HEPA filter fan.
4. Lean the right support wall against the right side of the
chamber and slide it up carefully into vertical position under
the top duct. The three parts should lock firmly into place. Do
not install the HEPA filter (Section 5.5.2) until the shelf
assembly is complete.
5. Position a shelf bracket in the slots on the wall, with the back
flange facing up and the side flange facing down. Then pop
the shelf bracket up into the slots.
6. Install another shelf bracket in the same location on the
opposite wall of the incubator.
7. Slide a shelf into the clips.
Figure 3. Shelving Detail
5.5.2 HEPA Filter
You can use a HEPA filter to remove airborne particulates from
the chamber environment. The filter provided is 99.97% efficient
with a 0.3 micron rating. It should normally be replaced every 6
months, more often if the chamber environment has a relatively
high concentration of particulates.
To install the filter, place it in the hole in the top duct and twist,
as shown in Figure 4.
Figure 4. HEPA Filter
When the shelves and filter are in place, press to advance to
the next prompt
5.6 Water Jacket Fill
The next prompt asks you to fill the water jacket. Since water
level is monitored automatically by the incubator control
program, you will not see this prompt if the water level sensor
detects that the jacket is already full.
Before starting to fill the water jacket, have a water source or
10 gallons of water available near the incubator. Also, make sure
that the drain valve (located on the lower left side of the unit) is
closed.
For double (stacked) units, always fill the bottom unit first.
WARNING! Over-filling the water jacket may result
in damage to electronic components and can become
hazardous. Avoid filling beyond the weep hole located
near the upper left corner of the glass door.
The fill port is located on the front of the unit behind the upper
left corner of the inner glass door. It is labeled FILL. To fill the
water jacket, complete the following steps:
1. Locate the plastic bottle of water conditioning crystals,
funnel, and length of 3/8 inch tubing supplied with the
incubator.
2. Connect one end of the 3/8 inch tubing to the funnel.
Figure 5. Water Jacket Fill
Ta b
Tab
HEPA
filter
Top
duct

Installation and Operation
Ultima II and Elite II Incubators
6
3. Fill the plastic bottle of water conditioning crystals with
warm water, replace the cap, and shake the bottle until all the
crystals are dissolved.
Note: It is important that you use the entire bottle of crystals to
treat the water in the water jacket. If all of the crystals
are not dissolved on the first try, pour off and keep
shaking the bottle until all the crystals are dissolved
4. Open the outer door of the incubator and remove the fill port
cap located in the upper left corner (labeled FILL).
5. Slide the tubing to the fill fitting as shown in Figure 5.
6. Carefully pour all of the conditioning crystal solution into the
funnel.
7. Using cool tap water, continue to fill the water jacket
(approximately 10 gallons).
–Always use cool tap water to fill the water jacket. If you
use hot water to fill the water jacket, temperature
correlation will be impossible for days.
–Do not use deionized water to fill the water jacket.
–If the water jacket is too full, water will come out of the
weep hole. If that happens, remove any excess water from
the water jacket, using the drain valve at the bottom of the
incubator (refer to Section 8.3 on page 14.)
8. Replace the fill port cap.
9. Close both incubator doors; when the water jacket is full, the
program automatically advances to the next prompt.
5.7 Re-level Incubator
Level the incubator left to right and front to back (refer to
Section 5.4 on page 4). press to advance to the next prompt.
5.8 Set Up CO2Supply
This step will be skipped if internal devices already detect
adequate input pressure.
CO2gas connection(s) are located at the rear of the unit.
Note: The required gas supply pressure is 15 PSI. Make sure
the supply is available in the installation area.
To install the CO2connections, complete the following steps:
1. Be sure that you have the right size tubing (3/16 in. interior
diameter).
2. Cut a small length (approximately 6 in.) from the supplied
roll of 3/16 in. ID tubing.
3. Pull the cap off the gas connection port at the rear of the
incubator and attach one end of the tubing to the gas
connection port.
4. Connect the opposite end of the tubing to a high-quality input
gas filter (0.3 micron supplied). Note that the side of the filter
marked “IN” faces the incoming gas connection, not the back
of the incubator (refer to Figure 6).
5. Use the remaining 3/16 in. ID tubing to connect the input gas
to the gas filter. Keep the tubing length as short as possible to
minimize the pressure drop.
Figure 6. Gas Connections
6. Repeat steps 1-5 for the second gas connection. Note that
stacked units require separate gas connections.
5.9 Set Up O2or N2 Supply (Tri-Gas Units Only)
For tri-gas units, the gas you hook up at T3 and T4 depends on
whether the ambient oxygen level is above or below the desired
O2setpoint.
If the O2(oxygen) setpoint is greater than ambient, hook up O2
supplies (enhancement mode).
If the O2setpoint is less than ambient, hook up N2(nitrogen)
supplies (depletion mode).
In both case, hook up the gas connections in a similar way to the
CO2connections described in Section 5.8. Use connections T3
and T4 as shown in Figure 6. The right connections to use will be
shown in the display prompts.
WARNING! Never use oil to lubricate fittings used
for O2connections. Oil easily combusts in the
presence of high concentrations of oxygen.
When the gas supplies are properly connected and the supply is
turned on, the display will automatically advance to the next
prompt.
Gas Filter
3/16 in. ID Tubing
T1
T2 T4
T3

Installation and Operation
Ultima II and Elite II Incubators
7
5.10 Humidity Pan
Do not install the humidity pan during this special start-up
procedure.
1. After you have entered the appropriate operating parameters
and completed the initial start-up procedure, wait
approximately eight hours for the cabinet temperature to
stabilize.
2. Fill the humidity pan with cold water and place it in the
bottom of the chamber (refer to Figure 7 on page 7); then
press . to continue.
Note: To retard microorganism growth, you can use 0.25 ml of
Roccal II or the equivalent (1:15,000 ratio) in the
humidity pan. Do not use the water conditioning crystals.
Test the Roccal II for effects on cell growth prior to
applying to the humidity pan.
3. After installing the humidity pan, wait another four hours for
stabilization. Your incubator will then be ready to operate.
Figure 7. Humidity Pan
5.11 Setpoint Parameters
Once you have completed the previous installation steps, you
will then be prompted to accept or adjust the values of several
operating parameters. In each case, press the Increase or
Decrease buttons to change a displayed value, press Mode when
the displayed value is the one you want. The modifiable value is
always the one that is flashing.
If you do not adjust a displayed parameter and 60 seconds elapse,
the software will register the existing default value and move to
the next parameter.
If you want to operate with all the factory-set defaults, press
Mode repeatedly until you come to the end of the prompts.
The adjustable parameters are:
•Temperature (default 37ºC)
•Warm alarm (default 40ºC; cannot deviate from the
temperature setpoint by less than 0.3ºC)
•Cold alarm (default 34ºC; cannot deviate from the
temperature setpoint by less than 0.3ºC)
•CO2 setpoint (default 5%)
•High CO2alarm (default 6%; cannot deviate from the CO2
setpoint by less than 1%)
•Low CO2alarm (default 4%; cannot deviate from the CO2
setpoint by less than 1%)
•O2setpoint (tri-gas models only; default is typical
atmospheric, 20.9%)
•High O2alarm (tri-gas models only; default 23.9%; cannot
deviate from the O2setpoint by less than 1%)
•Low O2alarm (tri-gas models only; default 17.9%; cannot
deviate from the O2setpoint by less than 1%)
•HEPA filter change reminder (in months, 1, 2, or 3)
When you have finished adjusting the parameters, the screen will
return the normal display (shown on the next page). An
additional message will indicate that the stabilization period has
begun (normally 11 to 12 hours).
During this initial stabilization period, leave the door closed.
Humidity Pan