REVCO Ultima II User manual

305978H02 Rev A
©2003
All rights reserved
Printed in U.S.A.
Ultima II and Elite II
Laboratory CO2Incubators
Installation and Operation Manual
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . 2
Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Initial Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Key Switch and Control Operation . . . . . . . . . . . . . 8
Control Systems Theory . . . . . . . . . . . . . . . . . . . . 13
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . 19

Installation and Operation
Ultima II and Elite II Incubators
i
Table of Contents
1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1 Features and Benefits . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
1.2 Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1
2 Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
3 Operating Standards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
4 Pre-Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
4.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.1.1 Included Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.2 Set-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.3 Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2
4.4 Gas Supplies . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
4.5 Wiring . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3
5 Initial Start-Up Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
5.1 Start-Up Prompts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.2 Installation Tools . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.3 Power and Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.4 Level and Position . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4
5.5 Shelving, HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.5.1 Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.5.2 HEPA Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.6 Water Jacket Fill . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5
5.7 Re-level Incubator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.8 Set Up CO2 Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.9 Set Up O2 or N2 Supply (Tri-Gas Units Only) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6
5.10 Humidity Pan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7
5.11 Setpoint Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7

Installation and Operation
Ultima II and Elite II Incubators
ii
6 Key Switch and Control Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
6.1 Key Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
6.2 Control Panel Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8
6.3 Display Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
6.4 Setpoint and Alarm Programming Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9
6.5 Service Mode Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
6.6 Restore Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10
6.7 Alarm Summary . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
6.8 Calibration (Optional) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.8.1 CO2Measurement and Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.8.2 Temperature Correlation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
6.9 Remote Alarm Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
7 Control Systems Theory . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
7.1 Temperature Control System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
7.2 Overtemperature Monitoring System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
7.3 Door Heat System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .13
8 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
8.1 Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.2 CO2Filter Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.3 Draining the Water Jacket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .14
8.4 Decontamination Kit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .15
8.5 Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
8.6 Door Seal Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .16
9 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
10 Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19

Installation and Operation
Ultima II and Elite II Incubators
1
1 Introduction
Figure 1. Ultima II and Elite II Incubators
1.1 Features and Benefits
Revco UltimaTM II and EliteTM II incubators are designed to
create a stable, reliable environment for cell culture applications.
They operate at temperatures ranging from 5°C above ambient
temperature to +40°C.
•Convenience Outlet. A receptacle rated at 115 VAC, 75 watts
maximum, is located at the rear of the incubator. The outlet is
ideal for electronic recorders or other small loads. Do not
exceed the electrical capacity of this outlet.
•Alarms:
–High and low level CO2and (on Ultima tri-gas models) O2
setpoint alarms, adjustable to within ±1% of setpoint.
–High and low temperature alarms settable by the user.
•Chamber Drain. All models are provided with a chamber
drain valve at the bottom left of the cabinet.
•CO2tank switchover and Alarm.
•IntrLogicTM II microprocessor control system with large,
easy-to-read display. On program request, software steps the
user through initial installation and startup procedures.
•Keyed power/alarm switch provides setpoint security.
•Units stackable.
•An in-chamber HEPA filter is standard for Ultima models,
optional for Elite models.
•The IncuSoftTM interface for RS485 data port
communications is standard for Ultima models, optional for
Elite models.
1.2 Requirements
•High quality two-stage, low pressure 30 psig pressure
regulators are required for proper operation of the CO2and
(if applicable) O2gas supplies, 15 psig at the incubator.
•CO2used in the incubator must be at least 99.9% pure.
•In-line gas supply filters must be used on the CO2supply and
(on tri-gas units) the O2supply to prevent damage to the
solenoid valve(s). One filter for each gas is supplied with the
startup kit:
–Type: Microbiological
–Specification: 0.3 micron
–Location: rear of unit
•Each chamber contains approximately 10 gallons of jacket
water when filled. The water takes six to eight hours to heat
from 20°to 37°C. This time must be allowed for the interior
temperature to stabilize before any adjustments or permanent
calibrations are made.
Welded Wall
Seamless Wall

Installation and Operation
Ultima II and Elite II Incubators
2
2 Safety Precautions
In this manual and on labels attached to this product, the words
WARNING and CAUTION mean the following:
•WARNING: a potentially hazardous situation which, if not
avoided, could result in serious injury or death.
•CAUTION: a potentially hazardous situation which, if not
avoided, may result in minor or moderate injury or damage to
the equipment.
The following symbols are used in caution, warning and
informational labels attached to the incubator:
Before installing, using or maintaining this product, please be sure
to read this manual and product warning labels carefully. Failure to
follow these instructions may cause this product to malfunction,
which could result in injury or damage.
Below are important safety precautions that apply to this product:
•Use this product only in the way described in the product
literature and in this manual. Before using it, verify that this
product is suitable for its intended use.
•Do not modify system components, especially the controller.
Use OEM exact replacement equipment or parts. Before use,
confirm that the product has not been altered in any way.
•Disconnect the unit from all power sources before cleaning,
troubleshooting, or performing other maintenance on the
product or its controls. To disconnect power supply to the
incubator, unplug the supply cord at the back of the incubator.
Note that turning the key switch on the front control panel to
the Off position is not sufficient to disconnect power.
WARNING! The user is responsible for carrying out
appropriate decontamination procedures when
hazardous materials are spilled on or inside the
incubator.
3 Operating Standards
The incubators described in this manual are classified for use as
stationary equipment in a Pollution Degree 2 and Overvoltage
Category II environment, according to the UL61010A-1 and IEC
664 standards.
These units are designed to operate under the following
environmental conditions:
•Indoor use
•Altitude up to 2000m
•Maximum relative humidity 80% for temperatures up to 31ºC
•Main supply voltage fluctuations not to exceed 10% of the
nominal voltage.
4 Pre-Installation
4.1 Unpacking
At delivery, examine the exterior for physical damage while the
carrier’s representative is present. If exterior damage is present,
carefully unpack and inspect the unit and all accessories for
damage. If there is no exterior damage, unpack and inspect the
equipment within five days of delivery. If you find any damage,
keep the packing materials and immediately report the damage to
the carrier. Do not return goods to the manufacturer without
written authorization. When submitting a claim for shipping
damage, request that the carrier inspect the shipping container
and equipment.
4.1.1 Included Parts
The following items are packaged and shipped inside the
incubator cabinet:
•Shelves and shelf brackets
•3/16 in. (476 mm) ID clear tubing for the gas connection (a Y
fitting is also supplied with dual-chamber units)
•Decontamination kit: 0.3 micron gas filter, replacement
Blower Wheel, CO2Sensor gasket, and Injection Port
Assembly gasket
•Tubing, funnel, and bottle of water conditioning crystals for
filling the water jacket
•Humidity pan
•HEPA filter (if one comes with your unit)
•A thin, 1/2” open-end wrench to adjust leveling legs and
relocate the outer door hinge
•Stacking brackets (for dual models)
4.2 Set-up
Remove shelves from the inside of the incubator and clean the
incubator (refer to Section 8.1 on page 14). The shelves and
brackets can be autoclaved at this time.
4.3 Location
Install the unit in a level area free from vibration with a minimum
of three inches (7.6 cm) of space on the sides and rear and
12 inches (30.5 cm) at the top. The floor must be able to support
40 PSI (single chamber incubator) or 75 PSI (double chamber
incubator).
Be sure to position the incubator so that the power cord can
easily be reached to disconnect power. Do not position the
equipment in direct sunlight or near any HVAC duct/diffusers.
The ambient temperature range at the location must be 59 to
90°F (15 to 32°C).
caution, info
electrical hazard
hot surface
earth ground
protective conductor terminal
on
off
alternating
current

Installation and Operation
Ultima II and Elite II Incubators
3
4.4 Gas Supplies
Verify that the incubator gas supplies are available near the
installation area: CO2for all incubators, also O2 and N2for tri-
gas models. The required gas supply pressure is 15 PSI,
controlled by a high-quality, two-stage regulator suitable for each
connected input gas.
Do not connect the gas at this time.
4.5 Wiring
CAUTION! Connect the equipment to the correct
power source. Be sure to operate the incubator at the
voltage specified on the dataplate. Incorrect voltage
can result in severe damage to the equipment.
WARNING! For personal safety and trouble-free
operation, this unit must be properly grounded before
it is used. Failure to ground the equipment may cause
personal injury or damage to the equipment. Always
conform to the National Electrical Code and local
codes. Do not connect the unit to overloaded power
lines.
The incubators described in this manual are rated either for
supply voltage 115/120 VAC, single-phase, 60Hz, 6 amps, or for
supply voltage 230 VAC, single-phase, 50Hz, 3 amps. Be sure to
check your dataplate for correct information.
Always connect the unit to a dedicated (separate) circuit. Do not
exceed the electrical and temperature ratings printed on the
dataplate located near the upper hinge of the unit. Electrical
codes require fuse or circuit breaker protection for branch circuit
conductors. Use time delay fuses for #12 AWG circuits.

Installation and Operation
Ultima II and Elite II Incubators
4
5 Initial Start-Up Procedures
Figure 2. Control Panel: First Start-Up Prompt
IMPORTANT NOTE: When setting up your incubator for the
first time, be sure to follow the control instructions on the
sticker provided on the control panel, and in Section 5.3
below.
5.1 Start-Up Prompts
When you first start up your incubator, the control display will go
through several prompts that will guide you through the entire
set-up process, from leveling the incubator to adjusting the
operating parameters.
During normal operation, the control panel display looks like
Figure 8 on page 8. The next chapter explains all the control
functions available during normal operation. On initial start-up,
however, you will see a series of prompts similar to the one
shown above. This chapter explains the start-up prompts and the
procedures they ask you to follow.
5.2 Installation Tools
Tools you will need to complete installation are:
•A sharp knife or scissors suitable for cutting plastic tubing
•A bubble level, at least 9 in. (23 cm) long, for leveling the
incubator
•A thin, 1/2 in. (1.25 cm) open-end wrench (provided with
your incubator) to adjust the leveling feet
5.3 Power and Key Switch
Turn the key switch (shown in the figure above) to the “1”
position.
The Revco logo will then roll across the display.
While the logo is displayed, press and hold the Increase button
() until the logo disappears to initiate the special start-up
prompts. The first prompt is the one shown above: if you see a
temperature display instead, you haven’t pressed the Increase
button long enough — turn the key switch back to “0” and try
again.
5.4 Level and Position
The first prompt asks you to level the incubator. It is very
important to level the unit both left to right and front to back.
1. Place a bubble level across the top of the incubator (side to
side).
2. Use the thin, 1/2 in.(1.25 cm) open-end wrench to adjust the
leveling feet located at the base of the incubator.
3. Repeat steps 1 and 2, this time placing the bubble level on top
of the incubator front to back.
4. Slide the incubator into place.
After the water jacket is filled, the incubator must be re-leveled
left to right and front to back.
When the incubator is level, press to advance to the next
prompt.
Key Switch
Mode
Level incubator
- complete

Installation and Operation
Ultima II and Elite II Incubators
5
5.5 Shelving, HEPA Filter
The next prompt asks you to install the shelves and the HEPA
filter.
5.5.1 Shelves
The incubator shelves are fully adjustable. First install the side
support walls and top duct. Then determine the location you want
for each shelf and install the shelf brackets (refer to Figure 3).
1. Place the base pan with the hole for the humidity pan facing
the front of the cabinet.
2. Place a shelf support wall on top of the base on the left side,
with the indicator hole toward the front bottom of the unit.
The curve of the support wall aligns with the curve of the
chamber.
3. Install the top air duct, making sure that the circular hole is to
the right directly under the HEPA filter fan.
4. Lean the right support wall against the right side of the
chamber and slide it up carefully into vertical position under
the top duct. The three parts should lock firmly into place. Do
not install the HEPA filter (Section 5.5.2) until the shelf
assembly is complete.
5. Position a shelf bracket in the slots on the wall, with the back
flange facing up and the side flange facing down. Then pop
the shelf bracket up into the slots.
6. Install another shelf bracket in the same location on the
opposite wall of the incubator.
7. Slide a shelf into the clips.
Figure 3. Shelving Detail
5.5.2 HEPA Filter
You can use a HEPA filter to remove airborne particulates from
the chamber environment. The filter provided is 99.97% efficient
with a 0.3 micron rating. It should normally be replaced every 6
months, more often if the chamber environment has a relatively
high concentration of particulates.
To install the filter, place it in the hole in the top duct and twist,
as shown in Figure 4.
Figure 4. HEPA Filter
When the shelves and filter are in place, press to advance to
the next prompt
5.6 Water Jacket Fill
The next prompt asks you to fill the water jacket. Since water
level is monitored automatically by the incubator control
program, you will not see this prompt if the water level sensor
detects that the jacket is already full.
Before starting to fill the water jacket, have a water source or
10 gallons of water available near the incubator. Also, make sure
that the drain valve (located on the lower left side of the unit) is
closed.
For double (stacked) units, always fill the bottom unit first.
WARNING! Over-filling the water jacket may result
in damage to electronic components and can become
hazardous. Avoid filling beyond the weep hole located
near the upper left corner of the glass door.
The fill port is located on the front of the unit behind the upper
left corner of the inner glass door. It is labeled FILL. To fill the
water jacket, complete the following steps:
1. Locate the plastic bottle of water conditioning crystals,
funnel, and length of 3/8 inch tubing supplied with the
incubator.
2. Connect one end of the 3/8 inch tubing to the funnel.
Figure 5. Water Jacket Fill
Ta b
Tab
HEPA
filter
Top
duct

Installation and Operation
Ultima II and Elite II Incubators
6
3. Fill the plastic bottle of water conditioning crystals with
warm water, replace the cap, and shake the bottle until all the
crystals are dissolved.
Note: It is important that you use the entire bottle of crystals to
treat the water in the water jacket. If all of the crystals
are not dissolved on the first try, pour off and keep
shaking the bottle until all the crystals are dissolved
4. Open the outer door of the incubator and remove the fill port
cap located in the upper left corner (labeled FILL).
5. Slide the tubing to the fill fitting as shown in Figure 5.
6. Carefully pour all of the conditioning crystal solution into the
funnel.
7. Using cool tap water, continue to fill the water jacket
(approximately 10 gallons).
–Always use cool tap water to fill the water jacket. If you
use hot water to fill the water jacket, temperature
correlation will be impossible for days.
–Do not use deionized water to fill the water jacket.
–If the water jacket is too full, water will come out of the
weep hole. If that happens, remove any excess water from
the water jacket, using the drain valve at the bottom of the
incubator (refer to Section 8.3 on page 14.)
8. Replace the fill port cap.
9. Close both incubator doors; when the water jacket is full, the
program automatically advances to the next prompt.
5.7 Re-level Incubator
Level the incubator left to right and front to back (refer to
Section 5.4 on page 4). press to advance to the next prompt.
5.8 Set Up CO2Supply
This step will be skipped if internal devices already detect
adequate input pressure.
CO2gas connection(s) are located at the rear of the unit.
Note: The required gas supply pressure is 15 PSI. Make sure
the supply is available in the installation area.
To install the CO2connections, complete the following steps:
1. Be sure that you have the right size tubing (3/16 in. interior
diameter).
2. Cut a small length (approximately 6 in.) from the supplied
roll of 3/16 in. ID tubing.
3. Pull the cap off the gas connection port at the rear of the
incubator and attach one end of the tubing to the gas
connection port.
4. Connect the opposite end of the tubing to a high-quality input
gas filter (0.3 micron supplied). Note that the side of the filter
marked “IN” faces the incoming gas connection, not the back
of the incubator (refer to Figure 6).
5. Use the remaining 3/16 in. ID tubing to connect the input gas
to the gas filter. Keep the tubing length as short as possible to
minimize the pressure drop.
Figure 6. Gas Connections
6. Repeat steps 1-5 for the second gas connection. Note that
stacked units require separate gas connections.
5.9 Set Up O2or N2 Supply (Tri-Gas Units Only)
For tri-gas units, the gas you hook up at T3 and T4 depends on
whether the ambient oxygen level is above or below the desired
O2setpoint.
If the O2(oxygen) setpoint is greater than ambient, hook up O2
supplies (enhancement mode).
If the O2setpoint is less than ambient, hook up N2(nitrogen)
supplies (depletion mode).
In both case, hook up the gas connections in a similar way to the
CO2connections described in Section 5.8. Use connections T3
and T4 as shown in Figure 6. The right connections to use will be
shown in the display prompts.
WARNING! Never use oil to lubricate fittings used
for O2connections. Oil easily combusts in the
presence of high concentrations of oxygen.
When the gas supplies are properly connected and the supply is
turned on, the display will automatically advance to the next
prompt.
Gas Filter
3/16 in. ID Tubing
T1
T2 T4
T3

Installation and Operation
Ultima II and Elite II Incubators
7
5.10 Humidity Pan
Do not install the humidity pan during this special start-up
procedure.
1. After you have entered the appropriate operating parameters
and completed the initial start-up procedure, wait
approximately eight hours for the cabinet temperature to
stabilize.
2. Fill the humidity pan with cold water and place it in the
bottom of the chamber (refer to Figure 7 on page 7); then
press . to continue.
Note: To retard microorganism growth, you can use 0.25 ml of
Roccal II or the equivalent (1:15,000 ratio) in the
humidity pan. Do not use the water conditioning crystals.
Test the Roccal II for effects on cell growth prior to
applying to the humidity pan.
3. After installing the humidity pan, wait another four hours for
stabilization. Your incubator will then be ready to operate.
Figure 7. Humidity Pan
5.11 Setpoint Parameters
Once you have completed the previous installation steps, you
will then be prompted to accept or adjust the values of several
operating parameters. In each case, press the Increase or
Decrease buttons to change a displayed value, press Mode when
the displayed value is the one you want. The modifiable value is
always the one that is flashing.
If you do not adjust a displayed parameter and 60 seconds elapse,
the software will register the existing default value and move to
the next parameter.
If you want to operate with all the factory-set defaults, press
Mode repeatedly until you come to the end of the prompts.
The adjustable parameters are:
•Temperature (default 37ºC)
•Warm alarm (default 40ºC; cannot deviate from the
temperature setpoint by less than 0.3ºC)
•Cold alarm (default 34ºC; cannot deviate from the
temperature setpoint by less than 0.3ºC)
•CO2 setpoint (default 5%)
•High CO2alarm (default 6%; cannot deviate from the CO2
setpoint by less than 1%)
•Low CO2alarm (default 4%; cannot deviate from the CO2
setpoint by less than 1%)
•O2setpoint (tri-gas models only; default is typical
atmospheric, 20.9%)
•High O2alarm (tri-gas models only; default 23.9%; cannot
deviate from the O2setpoint by less than 1%)
•Low O2alarm (tri-gas models only; default 17.9%; cannot
deviate from the O2setpoint by less than 1%)
•HEPA filter change reminder (in months, 1, 2, or 3)
When you have finished adjusting the parameters, the screen will
return the normal display (shown on the next page). An
additional message will indicate that the stabilization period has
begun (normally 11 to 12 hours).
During this initial stabilization period, leave the door closed.
Humidity Pan

8
Installation and Operation
Ultima and Elite Incubators
6 Key Switch and Control Operation
6.1 Key Switch
To operate the incubator with alarms activated, be sure to turn the key switch all the way to the rightmost position (as shown in the
figure below). To avoid nuisance alarms, work with the key switch in the “1” position during the set-up procedures described in
Section 5.
Figure 8. Control Panel, with RH display
6.2 Control Panel Features
The control panel is located on the top of your incubator.
Before loading and using your incubator, be sure to take some time to review the control panel functions.
The main display, during normal operation, shows: cabinet temperature in degrees Celsius, as measured by the sensor inside the cabinet;
and percentage levels of carbon dioxide (CO2) and relative humidity. When one or more of these fields flashes during normal operation,
an alarm or error condition is indicated. Note that alarm conditions are displayed only when the key switch is turned to the alarm
position.
When you are in programming mode (described in detail in Table 2) the main display changes to show the setpoint you are viewing and
related alarm setpoints. For example:
Here the first value displayed is temperature setpoint; the next two values are the warm alarm and cold alarm setpoints. The flashing
value is the one you can change using the increase and decrease buttons.
The Increase ( ) and Decrease ( ) pushbuttons are used to change setpoint values in programming mode and for various display
functions.
The Mode pushbutton is used: to silence an audible alarm; to enter programming and service modes; and in combination with the other
buttons for various display functions.
For full descriptions of display, programming, and service functions, refer to Tables 1, 2, and 3.
Key Switch
Mode
TEMP (C): 37.0
CO2 (%):
RH (%):
5.0
10.0
Set Temp SETPOINT:
37.0C 40.2C 33.8C

9
Installation and Operation
Ultima and Elite Incubators
6.3 Display Functions
Table 1. Control Panel Display Functions
6.4 Setpoint and Alarm Programming Functions
To enter programming mode, press Mode, and hold until the display indicates that you are changing to program mode. Pressing Mode
repeatedly scrolls through the available functions. Whichever parameter you are currently changing, the display will automatically
return to normal operating mode 30 seconds after the last key entry. You can also exit programming mode by scrolling through all
available functions and parameters using the Mode button.
Table 2. Setpoint and Alarm Programming Functions
Function Meaning Sequence Display
Normal operation Default display while incubator
is running — Display shows cabinet temperature, CO2, Relative
Humidity, O2.
Cold excursion Show coldest cabinet temperature
since last startup or reset Press Display shows cold excursion while button is pressed.
Warm excursion Show warmest cabinet temperature
since last startup or reset Press Display shows warm excursion while button is pressed.
Mode Silence audible alarm Press Mode Display shows current values, alarm or error field
continues to flash.
Reset Return to default display after
excursion or alarm condition
Press and
simultaneously
Excursion values are reset; display shows current values.
Display flashes twice
Water temperature Show water temperature as
measured by sensor in water jacket
Press and Mode
simultaneously Display shows water temperature.
Function Programming Sequence
Adjust temperature setpoint Enter programming mode by pressing Mode and holding for 5 seconds. On release, the current temperature
setpoint value flashes in the display; use and to adjust it.
Adjust warm alarm setpoint Press Mode again. The current warm alarm setpoint value then flashes in the display; use and to
adjust it. There is a minimum deviation of 0.3º C between each alarm setpoint and the temperature setpoint.
Adjust cold alarm setpoint Press Mode again. The current cold alarm setpoint value then flashes in the display; use and to adjust
it. There is a minimum deviation of 0.3º C between each alarm setpoint and the temperature setpoint.
Adjust CO2setpoint
Press Mode again. The display changes to display CO2setpoint and high and low CO2alarm setpoints, with
the operating CO2setpoint flashing. Use and to adjust it. There is a minimum deviation of 1% between
each alarm setpoint and the operating CO2 setpoint.
Adjust CO2high alarm setpoint Press Mode again. The current high CO2alarm setpoint then flashes; Use and to adjust it. There is a
minimum deviation of 1% between each alarm setpoint and the operating CO2 setpoint.
Adjust CO2low alarm setpoint Press Mode again. The current high CO2low setpoint then flashes; Use and to adjust it. There is a
minimum deviation of 1% between each alarm setpoint and the operating CO2 setpoint.
Adjust O2setpoint
(tri-gas only)
Press Mode again. The display changes to display O2setpoint and high and low O2alarm setpoints, with the
operating O2setpoint flashing. Use and to adjust it. There is a minimum deviation of 1% between each
alarm setpoint and the operating O2 setpoint.
Adjust O2high alarm setpoint
(tri-gas only)
Press Mode again. The current high O2alarm setpoint then flashes; Use and to adjust it. There is a
minimum deviation of 1% between each alarm setpoint and the operating O2 setpoint.
Adjust O2low alarm setpoint
(tri-gas only)
Press Mode again. The current high O2low setpoint then flashes; Use and to adjust it. There is a
minimum deviation of 1% between each alarm setpoint and the operating O2 setpoint.
Set Temp offset Press Mode again. The display changes to display temperature offset (for calibration purposes). Use and
to adjust it.
Set CO2offset Press Mode again. The display changes to display CO2 offset (for calibration purposes). Use and to
adjust it.

10
Installation and Operation
Ultima and Elite Incubators
6.5 Service Mode Parameters
From programming mode, you can enter service mode by pressing Mode and holding for 5 seconds.Pressing Mode repeatedly scrolls
through the available functions. For any modifiable parameter you can use and to adjust the value. The display automatically
returns to normal operating mode 30 seconds after the last key entry or after scrolling through all available functions and parameters.
Table 3. Service Parameters
In service mode, data and bar graphs are also displayed showing power usage, for diagnostic purposes.
6.6 Restore Function
When the incubator is first powered up, you can restore all factory-set program parameter defaults as follows:
Parameter Notes
Network Adr Network address (for RS485 applications). Default 238.
SN Serial number.
Check Sum Checksum to identify firmware version
Ambient Temp Ambient temperature in ºC, as measured by a sensor in back of the cabinet.
Water Templ Water jacket temperature in ºC.
Temp Offset For temperature setpoint calibration; default 0ºC.
CO2Offset For CO2setpoint calibration; default 0%.
O2Calibration For O2setpoint calibration; default 0.
Function Sequence Notes
Restore program defaults Press and simultaneously,
hold for five seconds
Restores original (factory-set) values of all program parameters; clears excursion
values. This is useful for tracking recent excursions (for example, minimum and
maximum operating temperatures within a one-day period).

11
Installation and Operation
Ultima and Elite Incubators
6.7 Alarm Summary
This section describes the alarm functions in detail.
Some important general notes:
•Alarms are activated only when the key switch is turned to the alarm position.
•Audible alarms may be silenced at any time by pressing MODE; in that case a full ringback (not a chirp) will occur in 15 minutes.
When audible alarms are no active, the ringback condition will clear automatically. Note, however, that has-been alarms can still be
active.
•‘Has-been’ alarms (indicating past alarm conditions) may be cleared by pressing and simultaneously. Be careful, however,
because this action also clears temperature excursions: you may want to view excursions before clearing has-been displays.
The following table describes all alarm functions. Not all alarms are available on all units: for example, O2alarms are only relevant to
tri-gas units.
Table 4. Incubator Alarm Functions
Alarm Visual Audible Remote Has-been Hysteresis Notes
Temperature Warm x x x x 0.2º C
Temperature Cold x x x x 0.2º C
CO2 High x x x x 0.0%
CO2 Low x x x x 0.0% Visual alarm is immediate but audible, remote, and
has-been are delayed 15 minutes
O2 High x x x x 0.0%
O2 Low x x x x 0.0% Visual alarm is immediate but audible, remote, and
has-been are delayed 15 minutes
RH High x x x x 0.0%
RH Low x x x x 0.0% Visual alarm is immediate but audible, remote, and
has-been are delayed 30 minutes
Ambient Warm x
Ambient Cold x
Power Fail x x x x
If the key switch is not in the alarm position, the
display will still be dimmed for backup battery
conservation.
Add Water x x x Checked every 2 seconds and displayed every 4
seconds
HEPA Reminder xWill display for 18 hours, then clear automatically
Battery Low xChecked every 4th, 12th and 20th hour
Door Open x x x If the door is open for 3 minutes, mute will be
interrupted
Tank 1 CO2 Main x x x
Audible and remote alarms active only when both
tanks are empty, else visual only
Tank 2 CO2 Backup x x x
Tank 3 O2/N2 Main x x x
Tank 4 O2/N2 Backup x x x

Installation and Operation
Ultima II and Elite II Incubators
12
6.8 Calibration (Optional)
6.8.1 CO2Measurement and Calibration
This method uses an instrument, such as the Fyrite, to read the
actual CO2% in the chamber. The display can be changed to
agree with the measured reading. The factory standard method,
using traceable gas standards, assures an accuracy of ±0.2%. The
Fyrite instrument obtains ±0.5% accuracy.
To use the Fyrite for CO2concentration measurement, complete
the following steps:
1. Wait at least 15 minutes after the CO2level arrives at setpoint
before attempting any concentration measurement.
2. Make sure that the wick in the Fyrite sample tube is moist
and make sure that the tubing is in good condition (no cracks
or cuts).
3. Hold the Fyrite upright and away from your face. Depress the
plunger valve briefly to vent the Fyrite and release the valve.
WARNING! Never depress the plunger valve when
the Fyrite is in the inverted position. Doing so causes
fluid, which is corrosive and contains poisonous
elements, to spill. Refer to the MSDS sheet contained
in the Fyrite instruction packet for additional
information.
4. Invert the Fyrite, holding it at a slight angle to drain the fluid
into the top reservoir.
5. Turn the Fyrite up to a 45 degree angle momentarily to allow
fluid droplet drainage into the bottom reservoir.
6. Hold the Fyrite completely upright and away from your face.
Depress the plunger valve briefly and release the valve.
7. Loosen the locknut at the rear of the scale. Slide the scale
until the top of the fluid column lines up with zero on the
scale. Tighten the locknut.
Note: When setting zero, hold the Fyrite vertically and level
with your eyes while sighting across the scale to the top
of the fluid column. Use this same procedure for
adjusting zero and reading percent CO2.
8. Remove the plug from the grommet in the glass door, and
insert the open end of the tube through the opening in the
grommet.
9. Holding the Fyrite in the upright position, place the sampling
rubber connector tip over the plunger valve on the Fyrite.
Purge the sample line by squeezing the aspirator bulb twice,
then depress the plunger valve firmly with the connector tip.
Squeeze and release the aspirator bulb 18 times. During the
18th squeeze and with bulb held deflated, release the
connector tip and the plunger valve.
Note: Always hold the Fyrite by the fins only to prevent
warming of the Fyrite fluid during analysis.
10.Invert the Fyrite until the fluid drains into the top reservoir,
then turn the Fyrite upright to drain the fluid into the bottom
reservoir. Repeat this step once.
11.Briefly hold the Fyrite at a 45 degree angle to allow the fluid
to drain into the bottom reservoir.
12.Hold the Fyrite completely upright and immediately read the
CO2percent on the scale at the point corresponding to the top
of the fluid column.
13. Wait a few minutes, then repeat the entire procedure (steps 3
through 12) once to confirm the reading.
To offset the CO2 % level, refer to the Service Parameters section
(Section 6.5 on page 10).
6.8.2 Temperature Correlation
The factory standard method of temperature calibration uses
instruments with an accuracy of ±0.1°C or better. To place an
offset on the displayed reading to match any other calibrated
temperature measuring instrument:
To offset the temperature, refer to the Service Parameters section
(Section 6.5 on page 10).
6.9 Remote Alarm Connection
Terminal strips for remote alarms are located on the back of the
control housing. The contacts are not powered from internal
circuits. These terminals provide normally open or normally
closed contacts for switching remotely powered alarms.

Installation and Operation
Ultima II and Elite II Incubators
13
7 Control Systems Theory
7.1 Temperature Control System
Laboratory CO2Incubators have a “jacket” between the
incubator chamber and the exterior wall of the unit. In water-
jacketed incubators, the jacket is filled with water.
Interior chamber temperature control is maintained by two
sensors. One sensor is located in the jacket and the other sensor is
located in the chamber air. Both sensors constantly signal the
electronic circuitry. The chamber air provides a reference point
while the jacket is being controlled. This circuitry recognizes that
jacket temperature is very slow to react to any change in either
ambient or chamber temperature but the chamber temperature
can change very rapidly due to door opening.
For example:
The chamber inner door is opened. The chamber air sensor
immediately signals a large drop in temperature but the control
recognizes that, unless there is a corresponding (smaller) drop in
jacket temperature, there is no need to increase the heat. The
system does nothing until sufficient time passes to measure how
fast the chamber air temperature is rising to meet the jacket
temperature after the door is closed. Heat is applied to the jacket
in short bursts. The rate of heat application changes as the circuit
monitors the two sensors. This control scheme is extremely
accurate and stable, with the ability to control within ±0.2°C of
the setpoint.
7.2 Overtemperature Monitoring System
This system is activated anytime the chamber air sensor detects a
temperature above the overtemperature setpoint, which should be
set no closer than 0.3°C above the chamber temperature. When
the system is activated, the jacket heater is turned off and both
audio and visual alarms are activated. Control is now effectively
switched to the overtemperature monitoring system. The
overtemperature sensor is the chamber air sensor which also
provides the signal for the digital display on the control panel.
7.3 Door Heat System
Heating the inner surface of the outer door with a low wattage,
large area heater provides enough radiant heat to the glass door to
control condensation. The micro-processor control operates the
door heater.

Installation and Operation
Ultima II and Elite II Incubators
14
8 Maintenance
8.1 Cleaning
The incubator can be easily cleaned and disinfected in about 30
minutes.
Be sure to use an appropriate disinfectant solution: Roccal II; its
Lysol equivalent, 5 milliliters per liter; or O-Syl in a one percent
solution. You should always dilute disinfectants with sterile,
distilled water.
CAUTION! Before using any cleaning or
decontamination method except for those
recommended in this manual, contact Technical
Service to verify that you will not damage the
equipment.
CAUTION! Do not use strong alkaline or caustic
agents, which can cause corrosion, rust and
pitting of stainless steel surfaces. Stainless steel
is corrosion-resistant but not corrosion-proof.
CAUTION! Do not use sodium hypochlorite
solutions such as Purex and Clorox. These can
also cause corrosion and pitting of stainless steel.
CAUTION! Do not use steel wool pads such as
Brillo; they deposit carbon particles in the
chamber.
When cleaning stainless steel, use the mildest cleaning procedure
that will do the job effectively. To avoid marring the surface,
always rub in the direction of the finish polish lines.
Do not use aromatic solvents to clean the cabinet interior:
residues could cause contamination of the cabinet environment.
To clean and disinfect your incubator:
1. Remove the shelves, support walls and pans.
2. Clean all interior surfaces with the disinfectant solution using
a clean sponge.
3. Rinse the interior surfaces at least twice with sterile distilled
water.
4. Clean the inner door gasket thoroughly.
5. Clean the inside of the glass door with the disinfectant
solution, then rinse twice with sterile distilled water.
6. Clean the shelves, support walls and pans with disinfectant
and rinse thoroughly with sterile distilled water.
7. Wipe down all disinfected surfaces with an alcohol solution.
CAUTION! Alcohol is volatile and flammable.
Use only in a well-ventilated area removed from
open flames and other heat sources. Allow
sufficient time for fumes to dissipate before using
cleaned components.
8.2 CO2Filter Replacement
To replace the CO2Filter:
1. Turn the main power switch to OFF.
2. Turn the gas supply (or supplies) to OFF.
3. Remove the tubing from both ends of the gas filter.
4. Note the flow direction on the filter. The side marked IN
points to the gas supply. Install the new filter onto the tubing
connected to the incubator and the tubing connected to the
gas supply (refer to Section 4.4 on page 3 and Figure 6 on
page 6).
5. Turn the gas supply (or supplies) to ON.
6. Turn the main power switch to ON.
8.3 Draining the Water Jacket
A drain valve is located on the bottom left side of the unit. To
drain the water jacket:
1. Turn the main power switch to OFF.
2. Run a flexible plastic drain line to the nearest floor drain or a
large container.
Note: The jacket holds approximately 10 gallons of water.
Make sure that the floor drain or container can
accommodate that amount of water.
CAUTION! Water will rush out of the incubator
rapidly. Make sure that one end of the drain line is
located in a floor drain or large container before
connecting the other end of the drain line to the
drain fitting.
3. Attach the drain line to the water drain fitting on the lower
front of the incubator; this allows the jacket to drain.
4. Open the valve.

15
Installation and Operation
Ultima and Elite Incubators
Figure 9. Top Duct Components
8.4 Decontamination Kit
The decontamination kit provided with your incubator includes:
•a replacement blower wheel
•a CO2Sensor gasket
•and Injection Port Assembly gaskets.
To install replacement components (shown in Figure 9):
1. Turn off the main power supply by unplugging the service cord.
2. Turn the gas supply (or supplies) to OFF.
3. Remove the shelves and shelf brackets.
4. Tilt one duct wall toward the center of the incubator until it disengages from the top duct.
CAUTION! The top duct will drop down when the side wall is disengaged.
5. Remove the duct wall, the top duct, and the other duct wall.
6. Remove the blower wheel by carefully pulling firmly in a downward direction until the wheel slides off the motor shaft.
7. .Replace the blower wheel with the one provided in the decontamination kit. Make sure that the blower wheel spins freely and does
not come into contact with the motor mounting screws.
8. Carefully remove the thumbnuts from the sensor and injection plates, allowing to hang freely from the ceiling of the incubator.
Remove one plate at a time.
9. Carefully disconnect the wiring connectors or tubing from the plates.
10.Remove and discard the gaskets and replace them with the ones provided in the decontamination kit.
11.Replace the plates.
12.Reinstall the shelves and shelf supports.
13.Turn the main power switch to ON.
Injection Port
CO2Sensor
Blower Wheel
Assembly Gasket
Gasket

16
Installation and Operation
Ultima and Elite Incubators
8.5 Fuses
The fuses used in this incubator are located on the power supply board and should only be replaced by a qualified technician.
The F1 and F2 fuses are Type “T”, rated at 120V/6A or 230VAC/3A.
The F3 and F4 fuses are Type “T”, rated at 120V/500mA or 230VAC/300mA.
Refer to the Replacement Parts List on page 19 for service part numbers.
8.6 Door Seal Check
To check the door seal, complete the following steps:
1. Open the inner glass door.
2. Insert a strip of paper (a couple of inches wide) between the door gasket and the cabinet flange and close the door.
3. Slowly pull the paper strip from the outside. You should feel some resistance.
4. Repeat this test at 4 inch (10 cm) intervals around the door. If the door does not seal properly, call your Technical Service
representative: the gasket needs to be adjusted or replaced.

17
Installation and Operation
Ultima and Elite Incubators
9 Troubleshooting
WARNING! Troubleshooting procedures involve working with high voltages which can cause injury or death.
Troubleshooting should only be performed by trained personnel.
Table 5. Troubleshooting Procedures
Symptom Solution
General
No display on control panel.
No power:
• The service cord is loose in the outlet.
• Check the outlet for power.
• Check the circuit breaker in the power panel. Make sure the circuit serving the incubator is a dedicated
circuit. Reset the circuit breaker.
• Check that the main power switch on the incubator is turned on.
Water leaking from the cabinet.
Water jacket is overfilled:
• Visually check the water level.
• Drain some water from the water jacket.
Temperature display disagrees with setpoint.
• Check the jacket water level. The water must be up to the fill port.
• Allow sufficient time for stabilization. .
• Make sure door was not recently opened. If door was opened, allow stabilization time.
• Make sure power did not fail recently. If power failed, allow stabilization time after power is restored.
High temperature alarm flashes.
• Check the incubator location.
• Do not open the door more frequently than at five minute intervals. Set the operating temperature
setpoint at least 5°C above the maximum ambient temperature.
Oxidation forming on interior surfaces.
• Do not wipe surfaces with a Clorox solution greater than 10%.
• Always wipe surfaces with a mild detergent and water to remove Clorox residue.
• Never use a Brillo pad on interior surfaces. Use a non-metallic pad (e.g., Scotch-brite).
• Corrosive culture medias can cause oxidation.
Add-Water indicator is illuminated and alarm is
sounding.
• Do not fill the incubator with de-ionized water. Use tap or distilled water.
• Check the floor for evidence of a leak.
• Make sure the incubator is level. .
• Check that water is visible at the weep hole. If not, add water
Condensation
Humidity is too low.
• Close the glass door for at least 30 minutes.
• Make sure that the humidity pan is stainless steel, has at least 150 square inches of water surface
area, and more than one inch of water depth.
• Check the water level in the humidity pan. .
• Check the jacket water level.
• Make sure the glass door seals properly. Use the outer door seal procedure to check the inner door
seal
Too much condensation on glass door. • Outer door gasket is not sealing properly or is damaged.
• Make sure that the humidity pan cover is in place.
Condensation on inside of outer door. •Door seal is not tight.
Condensation on interior walls/ceiling.
• Check the water level.
• Make sure the incubator is level.
• Check the incubator location.
• Make sure the glass door seals properly. Use the outer door seal procedure to check the inner door
seal.
• Make sure that the humidity pan cover is in place.
Temperature Control
Temperature display is drifting. • Check the incubator location.
Inside temperature is not equal to the display
temperature.
• Verify the calibration procedure.
• Check the incubator location.
Temperature not controlling at setpoint. • Close the doors for a minimum of 30 to 40 minutes.
• Check the setpoint..
Temperature is over-shooting during the day. • Do not open the door more frequently than at five minute intervals.
• Check the incubator location.