Sakura Tissue-Tek Film 4740 User manual

© 2013 Sakura Finetek USA, Inc.
All Rights Reserved
Printed in U.S.A.
0002470-01 Rev. F
Manufactured for:
Sakura Finetek USA, Inc., Torrance, CA 90501 U.S.A.
Sakura Finetek Japan Co., Ltd., Tokyo, 135-0007, Japan
Sakura Finetek Europe B.V., 2408 AV Alphen aan den Rijn, NL

TABLE OF CONTENTS
Revised 08/08/2013 iii
Section Page
1. INTRODUCTION 1.1
Intended Use ................................................................................................................................................1.1
Safety Instructions........................................................................................................................................1.1
Safety Precautions .......................................................................................................................................1.2
General Description......................................................................................................................................1.1
Physical Characteristics................................................................................................................................1.3
Description of Control Panel.........................................................................................................................1.7
Specifications ...............................................................................................................................................1.8
2. INSTALLATION 2.1
General Information......................................................................................................................................2.1
Environmental Factors..................................................................................................................................2.1
Unpacking.....................................................................................................................................................2.1
Preparation of Slides Prior to Coverslipping ...............................................................................................2.10
3. OPERATING INSTRUCTIONS 3.1
Loading of Slides ..........................................................................................................................................3.1
Preparation of Film .......................................................................................................................................3.1
Storage of Unused Film................................................................................................................................3.1
Routine Operation ........................................................................................................................................3.1
Setup Procedures.........................................................................................................................................3.2
Coverslipping................................................................................................................................................3.5
Operating Precautions..................................................................................................................................3.6
4. SLIDE HANDLING 4.1
Handling of Coverslipped Slides...................................................................................................................4.1
5. ACCESSORIES 5.1
Standard Accessories...................................................................................................................................5.1
Optional Accessories....................................................................................................................................5.1
6. CARE OF THE INSTRUMENT 6.1
General Maintenance ...................................................................................................................................6.1
7. TROUBLESHOOTING 7.1
General Information......................................................................................................................................7.1
Power Failure ...............................................................................................................................................7.1
Messages .....................................................................................................................................................7.2
8. SERVICE AND REPLACEMENT PARTS 8.1
Service Information.......................................................................................................................................8.1
Replacement Accessory Items .....................................................................................................................8.1
Optional Accessory Items.............................................................................................................................8.1
Apendix A
Bar Code Reader..............................................................................................................................................A.1

Revised 08/08/2013 1.1
Section 1
INTRODUCTION
Intended Use
The Tissue-Tek®Film®Automated Coverslipper is
designed for the purpose of coverslipping human and
animal tissue specimens on slides.
The instrument, as part of the histopathology process,
is intended to facilitate the in vitro examination of human
and animal tissue for morphology changes by a
pathologist.
General Description
The Tissue-Tek®Film®Automated Coverslippers
designed to coverslip biological specimens that are
mounted on standard 25 x 75 mm (1 x 3 inch) microscope
slides (Figure 1-A). The use of a special, resin-coated, film
and xylene eliminates the need for cover glass and
mounting media. (Sakura can only recommend the use of
Tissue-Tek®Coverslipping Film because of its proven
performance). The film length is user-defined in four
selectable options: 45 mm, 50 mm, 55 mm, and 60 mm.
Coverslipping is performed at approximately 3 seconds
per slide with continuous processing of up to 3 pre-loaded
baskets. By linking the Film Coverslipper to the Tissue-
Tek®Prisma®Slide Stainer, the series of slide preparation
from slide staining to coverslipping is automatically
performed. A microcomputer controls the mechanical
movements, as well as the positioning and length of the
coverslipping film. An LCD offers a visual display of
selectable options and instrument status. All operating
functions are controlled through a control panel.
Adjustments to the software, such as xylene dispensing
volume and film length can be easily modified using the
function keys on the display panel.
Specimen slides ready for coverslipping are placed
into compatible baskets and loaded into the basket
loading station. The loading station should contain xylene
in order to protect the specimens from drying out prior to
coverslipping. The loading station can accommodate 3
baskets (60 slides) at one time. When the loading door is
closed, sensors detect the number of baskets placed in
the loading station. A robotic arm picks up one basket at a
time and rises to the coverslipping area. Each slide is
ejected from the basket where a pre-determined amount
of xylene is dispensed onto the slide. The coverslipping
film is applied to the slide and the slide is then returned to
its original position in the slide basket. When all of the
slides in the basket have been coverslipped, the basket is
transferred to the 12-position unloading unit. Baskets can
be removed immediately or stored until 12 baskets have
been placed in the unloading area. Use of an optional
automatic transfer link station enables the coverslipper to
receive slides directly from the Tissue-Tek®Prisma®
Automated Slide Stainer.
Figure 1-A
The Tissue-Tek Film Automated Coverslipper with
Code Reader, product code 4743, includes a bar code
reader installed inside the unit. The 4740 version of the
Tissue-Tek Film Automated Coverslipper arrives pre-wired
for a bar code reader which may be added later with the
purchase of product code 6508. This addition requires
installation by a Sakura Service professional. Please refer
to Appendix A for further instructions pertaining to
bar code reader functions.

INTRODUCTION
1.2 Revised 08/08/2013
Safety Instructions
The Tissue-Tek®Film®Automated Coverslipper
operating manual includes important instructions and
information related to the operating safety and
maintenance of the instrument. It is important to read the
operating manual carefully prior to the operation of the
instrument for the first time and to keep the manual with
the instrument at all times.
This instrument has been built and tested with the
following safety regulations on electrical measuring
control, regulating and laboratory devices:
IEC 61010-1 2nd Ed.
CAN/CSA C22.2 No. 61010-1 2nd Ed.
UL 61010-1 2nd Ed.
In order to ensure safe operation, the operator must
always comply with the instructions and warnings
contained in the operating manual.
CAUTION: Power Supply plug is used as mains power
disconnecting device. Locate unit where power
supply is easily accessible.
NOTE: The protective devices installed on both the
instrument and accessories may neither be removed nor
modified. Only authorized and qualified persons may
access and repair the internal components of the
instrument.
The safety devices installed in this instrument by the
manufacturer only establish the basis for accident
prevention. Primary responsibility for accident-free
operation lies, above all, with the institution that owns the
instrument and, in addition, the designated personnel that
operate, service, or repair the instrument.
Safety Precautions
CAUTIONS and NOTES are provided throughout this
manual to indicate levels of potential hazards or helpful
information as defined below.
CAUTION: Indicates a potential hazard in which failure
to follow instructions may result in damage to the
Prisma and/or other property.
NOTE: Indicates a reminder or other helpful information.
Safety Features
The Tissue-Tek Film Automated Coverslipper is
equipped with several safety features, which help to keep
the operator and the instrument safe from harm.
An alarm sounds when any of the following conditions
occur:
The loading door is open
The film door is open
The cover is open
Low solvent volume is detected
Film must be replaced
Air bubbles are detected in the solvent tube
When normal operation has been suspended due
to instrument failure
If a slide jam occurs, all mechanical movement
stops and an alarm will sound.
Regardless of the various safety features, prompt
attention to any potential problems can prevent damage to
specimens and/or slides if corrected immediately.

Section 1
Revised 08/08/2013 1.3
Physical Characteristics
Loading Area
Access to the loading area (Figure 1-B) is obtained by
opening the door located at the front, lower portion of the
instrument. The loading drawer can be accessed by gently
sliding the drawer out. This drawer should be filled with
xylene to prevent slides from drying out prior to
coverslipping. A maximum of three baskets can be loaded
into the drawer.
The Tissue-Tek Film Coverslipper accommodates slide
baskets compatible with the Tissue-Tek®DRS™2000 and
Prisma®Automated Slide Stainers.
Film Loading Area
Opening the film door, located on the left side of the
instrument, provides access to the film loading area
(Figure 1-C). Instructions for loading the coverslipping film
can be found on a diagram located on the back wall of the
instrument. The film is wound through positioning spools
and rollers until it has reached the coverslipping area. The
complex design of the film feeding system assures that
even after the end of the film is detected, all of the slides
held in the coverslipping position will be coverslipped. The
length of the film going from the film end sensor to the
cutter will coverslip 20 slides. The cutter blade used to cut
the film is exchangeable and should be replaced after
every 10 rolls of film (approximately 10,000 slides) or
whenever the film cut is ragged or torn.
Figure 1-B
Figure 1-C

INTRODUCTION
1.4 Revised 08/08/2013
Dispensing Mechanism
Xylene is pumped from the xylene bottle by a pump
and then dispensed onto the slide. The xylene bottle is a
glass bottle (Figure 1-D) with a capacity of 500 ml. It is
located on the bottom left of the instrument and can be
accessed by opening the film door. The xylene dispense
volume is adjustable through the control panel with five
user-selectable levels: 1 (min), 2, 3, 4, 5 (max). When the
xylene bottle becomes empty, all of the slides held in the
coverslipping position will be coverslipped. The tube
length assures a sufficient volume of xylene to coverslip
20 slides. In order to fill the xylene bottle, it must be
removed from the instrument.
The waste bottle is a glass bottle with a 200 ml
capacity and is used to collect excessive xylene drained
from the dispensing area. The waste bottle is located to
the right of the xylene bottle. It must be removed from the
instrument to be emptied.
Coverslipping Stage
The first basket is automatically lifted from the loading
drawer to the coverslipping stage (Figure 1-E). Slides are
pushed one at a time from the loaded slide basket by an
ejector arm onto the staging area. A pre-set volume of
xylene is dispensed on the slide and then the film is cut to
the appropriate length and positioned on the slide.
Unloading Area
After the film is applied to the slide, it is returned to the
slide basket in the same position from which it was
removed. As each slide is coverslipped and returned to
the basket, the basket is moved up one position until all
slides have been coverslipped. The basket is then
transported upward to the unloading area (Figure 1-F).
The unloading area is located on the top of the instrument
and is protected by a cover. It contains a carousel-like
storage unit that can hold up to 12 baskets (240
coverslipped slides). The baskets can be removed
individually as they are placed into the unloading area or
can be held until all 12 positions are filled. The 12-position
unloading unit containing baskets can be removed from
the instrument.
Figure 1-D
Figure 1-E
Figure 1-F

Section 1
Revised 08/08/2013 1.5
Fume Control
Xylene fumes are absorbed by a carbon filtration
system (Figure 1-G). The treated air is then force-vented
through the exhaust outlet. The two activated carbon
cartridges are located in the unloading area behind the 12-
position unloading unit. An external port (OD: 38 mm or 75
mm), an optional accessory, is available so the instrument
can be vented to an outside source. It is recommended
that the carbon filters be replaced after two weeks of
continuous use.
Power Switch
The instrument power is turned ON and OFF by
pressing the power switch (Figure 1-H) located on the
right front of the instrument on the control panel. The
power switch also acts as an emergency stop switch.
Figure 1-G
Figure 1-H

INTRODUCTION
1.6 Revised 08/08/2013
Rear of the Instrument
The power cord can be installed on the rear of the
instrument (..1.., Figure 1-I). The other end of the power
cord attaches into an appropriate grounded AC electrical
outlet. There are two fuses ..2.. located to the left of the
power cord inlet.
Also located on the rear of the instrument is the
connector port ..3.. for the link cable for the Tissue-Tek®
Prisma®Automated Slide Stainer.
Control Panel
All operating functions are controlled through the
control panel (Figure 1-J). There are nine keypad switches
and three LED’s interfacing with the instrument software.
A four-line LCD provides the user with information such
as, current status, setup menu option, error codes and
messages.
Rear Panel Outlets (Figure 1-I).
Figure 1-I
Figure 1-J
1
3
2

Section 1
Revised 08/08/2013 1.7
Description of Control Panel
Once the power is turned on, the instrument will
initialize and perform a series of system checks as it
prepares for operation.
After the initialization process is complete, the
instrument goes into standby mode and is ready for
operation. From the standby screen, a series of operations
can be selected.
Directly under the LCD display are three function keys
labeled: [Enter], [Menu], and [Exit] (Figure 1-K).
Figure 1-K
The [Enter] key is used to save changed settings and
to return to the previous screen. The [Menu] key takes the
user to the menu screen. It provides access to the
following functions:
Change dispensing volume
Change film length
Verify dispensing action
Reset user count
Set key sound
Select alarm sound
View software version #
The [Exit] key is pressed to return to the previous
screen without saving any changed settings.
Directly beneath these three keys are the left [<] and
right [>] arrow keys. The [<] key, when used in the menu
screen, moves the selection item upward or to the left.
(Holding the key down will cause a continuous action).
The [>] key moves the selection item downward or to the
right. (Holding the key down will cause a continuous
action).
On the left side of the control panel are two keys,
[Film] and [Prime]. After the film is loaded up to the
coverslipping staging area, pressing the [Film] key will
capture the film and begin to feed it automatically. The
[Prime] key is pressed to dispense the xylene using the
set xylene dispensing level.
Located on the right side of the control panel are the
[Stop] and [Start] keys. When the instrument is in
operation, the [Stop] key is used to stop operation already
in progress. It is also used to stop the xylene priming
action. The coverslipper will begin routine operation, start
of origin return action, or priming action when the [Start]
key is pressed.
There are three LED’s located on the control panel.
The Cover LED, the Load LED, and the Power LED. The
Cover LED lights when the cover on the top of the
instrument is ready to open. The Load LED lights when
the door of the loading station is ready to open. The
Power LED remains lit when power is being supplied to
the instrument.

INTRODUCTION
1.8 Revised 08/08/2013
Specifications
Models
Model
Name/Description
4740
Tissue-Tek®Film®Coverslipper (115 VAC) (USA)
4741
Tissue-Tek®Film®Coverslipper (100 VAC) (Asia)
4742
Tissue-Tek®Film®Coverslipper (230 VAC) (Europe)
4743
Tissue-Tek®Film®Coverslipper BCR (115 VAC) (USA)
Power Ratings and Requirements
Model
Voltage
Frequency
Amps
4740/4743
115 VAC ± 10% @ <15A
60 Hz
1.3A
4742
230 VAC ± 10% @ < 7A
50/60 Hz
0.8A
4741
100 VAC ±10% @ <15A
50/60 Hz
1.5A
Safety Standards
Tested and listed by Intertek Testing Services (ITS).
Complies with IEC 61010-1 2nd Ed., CAN/CSA C22.2 No.
61010-1 2nd Ed., UL 61010-1 2nd Ed.
CE registered in compliance with the requirements of
EMC Directive, LV Directive in Europe, In Vitro Diagnostic
Device Directive.
Dimensions
Centimeters: 72(W) x 58(D) x 69(H)
Inches: 28(W) x 23(D) x 27(H)
Instrument Weight
75 kg (165 lbs.)
Operating Conditions
Operating temperature: 10º C to 40º C (50º F to 104º F)
Relative humidity: 30-85% (non-condensing)
Atmospheric pressure: 70kPa to 106kPa
Protect from direct sunlight.
Storage Conditions
Storage temperature: -10º C to 60º C (º F to º F
Relative humidity: 30-95% (non-condensing)
Atmospheric pressure: 70kPa to 106kPa
Processing Speed
Maximum throughput: 1,080 slides per hour
60 slides/batch
Acceptable Slide Dimensions
Dimensions: 24.7 to 26.5 mm (W) x 74.7 to 76.5 mm (L)
Thickness: 0.9 to 1.2 mm thickness
Compatible Solvent
Reagent grade or analytical grade xylene only
Coverslipping Film
Width: 24 mm
Length: 45, 50, 55, and 60 mm
Material-Cellulose Triacetate with Resin and Back
Coatings
CAUTION: Xylene substitutes are not compatible for
use on the Tissue-Tek®Film® Coverslipper.

Revised 08/08/2013 2.1
Section 2
INSTALLATION
General Information
This section provides detailed installation and setup
instructions for the Tissue-Tek®Film®Automated
Coverslipper. The installation steps must be followed
correctly to ensure proper operation and service. Read
this operating manual carefully before attempting to
operate the instrument. Follow all instructions carefully.
The Tissue-Tek Film Coverslipper is a precision
instrument and must be handled accordingly. Rough
handling or dropping the instrument will disturb or damage
internal components. Always handle the instrument with
care.
Select a place where sufficient clearance can be
provided around the instrument. Ample working space is
necessary in front and on top of the instrument.
Environmental Factors
As with all sensitive electronic instruments, prolonged
exposure to excessive humidity and temperature should
be avoided. Temperature and humidity should be held
relatively constant. The ambient temperature for operating
the instrument is 10º C to 40º C (50º F to 104º F). The
ambient operating humidity range is 30-85% relative
humidity.
Unpacking
Removing the Outer shipper
The Tissue-Tek Film Coverslipper is packaged in a
sturdy cardboard shipping carton attached to a wooden
pallet (Figure 2-A).
Figure 2-A
1. Inspect the carton and make sure there are no visible
signs of damage. If visible signs of damage are
evident, immediately file a complaint with the carrier
and notify Sakura Finetek USA Technical Support
Department. In countries other than the USA, contact
the nearest authorized Sakura distributor or
representative.
2. If no visible signs of damage are evident, remove the
protective tie wraps securing the outer shipper box to
the pallet (Figure 2-B).
Figure 2-B

INSTALLATION
2.2 Revised 08/08/2013
3. Remove the lid of the box and remove the accessory
box positioned under the lid (Figure 2-C).
Figure 2-C
4. Remove the Styrofoam pieces surrounding the
instrument. The outer cardboard box may now be
lifted up and away from the instrument.
5. The instrument is positioned on the pallet and is
protected by a plastic cover and Styrofoam packing
material. If the Styrofoam has not already been
removed, remove it from the corners of the unit.
Remove the protective wrapping exposing the
instrument (Figure 2-D).
Figure 2-D
6. Carefully lift the coverslipper from the pallet and place
it on a sturdy, level surface capable of supporting 75
kg (165 lbs.).
CAUTION: The instrument is very heavy and large;
therefore, it is strongly recommended that it always
be lifted and transported by at least two people, one
positioned on each side of the instrument.
CAUTION: Always transport the instrument in an
upright position.
7. Remove any adhesive tape from the outside of the
instrument.
8. Open the loading area door and remove any packing
material.
9. Open the film door and carefully remove all adhesive
tape securing the components. Remove the
Styrofoam wedges and foam from the coverslipping
area (Figure 2-E). Be sure not to dispose of these
items until proper operation is verified.
Figure 2-E
NOTE: Be certain that all tape and foam is removed from
the instrument prior to operation.

Section 2
Revised 08/08/2013 2.3
Removing the Metal Packing Piece
CAUTION: Make sure that the instrument is switched
off and that the power cord is unplugged before
beginning.
1. Open the film door (located on the left side of the
instrument).
2. Locate the film waste tray. Directly to the right of the
film waste tray is a tagged piece of metal (Figure 2-F).
Figure 2-F
3. Cut the wire securing the tag.
4. Remove the metal piece holding the two rollers
(..1.., Figure 2-F).
NOTE: Failure to remove this metal piece will result in
instrument malfunction.
Unpacking the Accessories
When opening the accessory box, confirm that all
accessories have been included with the instrument:
Operating Manual (1)
Warranty Card (1) (located inside the Operating Manual)
(USA only)
Power Cord (1)
Slide Baskets (10)
Slide Basket Adapters (10)
Film (1 roll)
Cutter Blades (5)
Loading Drawer (1)
Loading Drawer Cover (1)
Xylene Bottle (1)
Xylene Bottle Cap (1)
Xylene Waste Bottle (1)
Xylene Waste Bottle Cap (1)
Spill Tray (installed) (1)
Waste Drain Guide (Funnel) (1)
12-position Unloading Unit (1)
Activated Carbon Filters (2)
Positioning the Instrument
Locate the instrument in a well-ventilated area,
avoiding exposure to corrosive vapors and extreme
variations in temperature or humidity. The area should be
clean, dust free, and have a firm, level surface capable of
holding at least 75 kg (165 lbs) of weight. Be sure it is
near a power source that meets the electrical
requirements specified on the rating label located on the
left of the instrument. The power outlet must be grounded
and it should be a dedicated line. Avoid proximity to direct
sunlight, open windows, sinks, ovens, hot plates, open
burners, or radiators.
Leveling the Instrument
Once the instrument is placed on a sturdy, level
surface, it is important that it is leveled. Using a wrench,
adjust the feet by turning the nut on each foot. Turning the
nut clockwise will raise the instrument, counter-clockwise
will lower the instrument.
Place a level on the inside, middle of the instrument
and adjust the feet until it is level.
Positioning the Accessories
The accessory items may be removed from the box
and positioned on the instrument.
1

INSTALLATION
2.4 Revised 08/08/2013
Installing the Activated Carbon Filters
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. Open the top cover of the instrument.
2. If the 12-position unloading unit is installed, remove it
by grasping the handle located in the middle of the
basket and lift upward.
3. Open the filter cover by lifting up the hook located at
the top center of the filter cover.
4. Lower the filter cover.
5. Remove the plastic wrapping from both carbon filters.
6. Place carbon filters horizontally in the appropriate
location (Figure 2-G).
Figure 2-G
7. Push firmly against the filter cover to close and latch
hook.
NOTE: It is recommended that the carbon filters be
replaced periodically. See Section 6, Care of The
Instrument, for maintenance schedule.
Installing the 12-Position Unloading Unit
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. Open the top cover to expose the unloading area.
2. Grasp the 12-position unloading unit by the handle
(Figure 2-H), located in the middle of the unit.
Figure 2-H
3. Locate the positioning hole on the instrument and line
it up with the peg on the underside of the unloading
unit.
4. Lower the 12-position unloading unit until it is sitting
correctly.
NOTE: The 12-position unloading unit is keyed to fit only
one way. If it does not sit properly, lift back up and
reposition.

Section 2
Revised 08/08/2013 2.5
Installing the Xylene Bottle
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. Confirm the xylene bottle is free from dust or debris. If
there is any debris inside the bottle, clean and dry
completely.
2. Fill the bottle with Xylene.
NOTE: Use high-grade xylene only (e.g., reagent grade
xylene)
3. Open the film door (located on the left side of the
instrument).
4. Locate the priming lid on the lower left side.
5. Lift priming lid and set the xylene bottle under the lid
by positioning the priming tube inside of the bottle
(Figure 2-I).
Figure 2-I
6. Set the bottle down and lower the priming lid until lid
is resting on top of the bottle.
7. Close the film door.
Installing the Xylene Waste Bottle
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. Open the film door located on the left side of the
instrument.
2. Position the xylene waste bottle in the designated
area next to the xylene bottle on the lower left side
(Figure 2-J).
Figure 2-J
3. Close the film door.

INSTALLATION
2.6 Revised 08/08/2013
Installing the Cutter Blade
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
CAUTION: Use caution when handling the blades;
even used blades are sharp.
1. Open the film door and locate the cutter blade handle
positioned in the coverslipping area.
2. Pull the spring steel tab on the blade assembly to the
right to clear the capture button on the black safety
handle.
3. The handle, along with the blade, can be removed
from the assembly by pulling it straight out. While
removing, hold the blade against the safety handle,
as it is not locked into place on the handle.
4. Lift the used blade off the safety handle and dispose
of in an appropriate container.
5. Insert a new blade into the recessed area of the
safety handle by aligning the hole in the blade to the
peg on the handle (Figure 2-K). Notice that the blade
fits into the handle in only one direction.
Figure 2-K
6. Holding the blade against the safety handle, carefully
insert the blade into the slit of the guide piece on the
blade assembly (Figure 2-L). Push the blade
completely in without dragging the sharp edge of the
blade along the guide piece. The black safety handle
must be seated flush against the guide piece when
the assembly is fully and correctly inserted. If
resistance is encountered prior to this point, carefully
use one finger of the opposite hand to press against
the body of the blade (near the tip) while continuing to
push the assembly in with the other hand.
Figure 2-L
7. When the blade is fully inserted, press the spring tab
over the capture button on the safety handle to
ensure it is seated securely in place
8. Close the film door.

Section 2
Revised 08/08/2013 2.7
Installing the Loading Station
CAUTION: Make sure that the instrument is switched
off and the power cord is unplugged before
beginning.
1. The loading station is positioned in the lower, right
section of the coverslipper. Open the door to expose
the loading area. Pull out the loading drawer.
2. Position the loading station along the guide rails in the
loading drawer (Figure 2-M).
NOTE: Failure to orient the loading station properly will
prevent the station from being secured on the drawer.
Figure 2-M
3. Place the sensor reactor fins in the loading station so
that they face the right hand side of the loading
station. Position them securely by engaging the
locating pins inside the loading station.
4. Fill the loading station with xylene up to the
designated line.
NOTE: Use high-grade xylene only (e.g., reagent grade
xylene)
5. Cover the loading station with the lid making sure to
position it so that the handle of the lid is next to the
sensor reactor fins.
NOTE: Lid must be removed prior to instrument operation.
6. Slide the loading drawer back into the instrument
being certain that the loading station is in the proper
position.
7. Close the loading door.
Installing the Waste Funnel
1. Open the film door located on the left side of the
instrument.
2. Position the funnel so that the narrow end is inserted
slightly into the waste Xylene hole (Figure 2-N).
Figure 2-N
3. Twist the wide end of the funnel so that the edge of
the funnel rests on the opening on the back wall.
4. Secure in place to make sure it is in the proper
position
Note: The funnel edge should fit snugly on the back wall
and should not move once installed.

INSTALLATION
2.8 Revised 08/08/2013
Installing the Waste Tray
1. Open the film door located on the left side of the
instrument.
2. Locate the guide rails for the waste tray underneath
the Xylene bottle and the Xylene waste bottle
3. Position the waste tray in between the guide rails
(Figure 2-O) and push the tray completely in.
Figure 2-O
Note: Check the waste tray frequently to make sure there
is no debris or Xylene.
Installing the Tissue-Tek Coverslipping
Film
1. Power the instrument on by pressing the power
switch to the “I” position.
2. Open the film door located on the left side of the
instrument.
3. Holding the film core made of white plastic with the
Tissue-Tek Film facing forward set the film in the
groove of the two film receiving rollers.
4. Thread the tip of the film outside of roller “1” and
through guide “2” (Figure 2-P).
Figure 2-P
5. Pass the film along the perimeter of roller “3”.
6. Pass the film over rollers “4” and “5” and then through
guide “6”.
7. Pass the film along the perimeter of roller “7”.
8. Insert the tip of the film into the final roller and press
the [Film] key to advance the film through the roller.
9. Release the [Film] key when the film is past the cutter
blade (3 to 6 mm) (1/8 to 1/4”)
NOTE: The cutter blade handle must be pressed down
when the “Press [FILM] to feed and cut film”message is
displayed.
NOTE: If the film jams while threading, turn the power to
the off position, pull the film back through the last roller,
power the instrument back on, and begin again with
step 4.
10. Remove the cut film with forceps or fingertips.
11. Close the film door.