Sakura Tissue-Tek Polar DM User manual

Operating Manual
Frozen Tissue Section Preparation Instrument
Cryostat Microtome

Introduction
The frozen tissue section preparation
instrument cryostat microtome Tissue-Tek®
Polar®(herein
after simply referred to as
"the Polar®
instrument") is an instrument
to section frozen tissues.
Carefully read this Operating Manual
and use the Polar®
instrument correctly
so that its performance can be utilized
fully.

a
Table of Contents
Safety Precautions............................................................................................ f
Chapter 1 Basic Knowledge of Instrument
Basic Knowledge.......................................................................................... 1-1
Explanation of System .........................................................................................................................1-1
Installation Method ....................................................................................... 1-2
Environmental Factors.........................................................................................................................1-2
Name of Each Part (Exterior) ....................................................................... 1-3
Name of Each Part (Left/Right Panels, Display Area) ................................ 1-5
Name of Each Part (Chamber) ..................................................................... 1-6
Handwheel..................................................................................................... 1-7
Overview of Handwheel.......................................................................................................................1-7
Coordination of Handwheel and Specimen Head...........................................................................1-7
Handwheel Lock tab.............................................................................................................................1-8
Unlocking the Handwheel...............................................................................................................1-8
Locking the Handwheel..................................................................................................................1-8
Foot Switch (DM only) .................................................................................. 1-9
Overview of Foot Switch ......................................................................................................................1-9
Connecting the Foot Switch.................................................................................................................1-9
How to Use/Lock the Foot Switch......................................................................................................1-10
Unlocking the Foot Switch............................................................................................................1-10
How to Operate the Foot Switch...................................................................................................1-10
Adjusting the Sectioning Speed....................................................................................................1-10
Locking the Foot Switch ...............................................................................................................1-10
How to Operate the Right Panel (DM only)................................................1-11
How to Operate the Right Panel for Motorized Sectioning.................................................................1-11
Starting Motorized Sectioning.......................................................................................................1-11
Pausing Motorized Sectioning......................................................................................................1-11
Stopping Motorized Sectioning.....................................................................................................1-11
Adjusting the Sectioning Speed....................................................................................................1-11
Types of Cryobars and Object Holders..................................................... 1-12
Types of Cryobar Removable Plates..................................................................................................1-12
Setting..........................................................................................................................................1-12
Types of Object Holders.....................................................................................................................1-12
Safety Functions......................................................................................... 1-13
Emergency Stop Switch.....................................................................................................................1-13
Actuating the Emergency Stop.....................................................................................................1-13
Resetting the Emergency Stop.....................................................................................................1-13
Blade Guard.......................................................................................................................................1-14
Recovery Processing.........................................................................................................................1-15
Specifications.............................................................................................. 1-16
Standard Accessories/Options.................................................................. 1-17
Explanation of Screens .............................................................................. 1-19
Explanation of Screen Layout............................................................................................................1-19
Explanation of Top of Screen........................................................................................................1-19
Explanation of Tab Area................................................................................................................1-19
Explanation of Tab Screen Area ...................................................................................................1-19
Explanation of Right-of-Screen Button Area.................................................................................1-20
Explanation of Status Display Area...............................................................................................1-20
Section Tab Screen..................................................................................... 1-21
FREE Mode Screen...........................................................................................................................1-21
Temperature Setting 1 Mode/Temperature Setting 2 Mode Screen ...................................................1-22

b
Motorized Sectioning Tab Screen (DM only) ............................................ 1-23
TRIM Mode Screen............................................................................................................................1-23
FINE Mode Screen.............................................................................................................................1-24
PAUSE Mode Screen.........................................................................................................................1-25
Tuning Tab Screen...................................................................................... 1-26
Utilities Tab Screen..................................................................................... 1-27
Other Items...................................................................................................................................1-28
History Tab Screen...................................................................................... 1-29
Various Settings.......................................................................................... 1-30
Setting the Section Thickness............................................................................................................1-30
How to Change in the Manual Sectioning Mode...........................................................................1-30
How to Change in the Motorized Sectioning Mode.......................................................................1-30
Setting the TRIM Thickness...............................................................................................................1-31
Registering/Editing in the Temperature Setting FREE Mode..............................................................1-32
How to Set the Chamber Temperature.........................................................................................1-32
How to Set the Specimen Head Temperature...............................................................................1-32
Registering/Editing in the Temperature Setting 1 / Temperature Setting 2 Mode...............................1-33
How to Set the Temperature.........................................................................................................1-33
Setting the Home Position Offset.......................................................................................................1-34
Setting the Sectioning End Position...................................................................................................1-35
Setting the Vacuum Suction Force.....................................................................................................1-36
Setting a Desired Suction Force...................................................................................................1-36
The suction force is fixed to 100%................................................................................................1-36
Setting the Date/Time ........................................................................................................................1-37
Setting the Date............................................................................................................................1-38
Setting the Time ...........................................................................................................................1-38
Setting a Schedule.............................................................................................................................1-39
Explanation of Always-on Operation Setting Screen....................................................................1-39
Setting the Cleaning Operation (Defrost Cycle/Ozone cycle) Start Time......................................1-40
Explanation of Scheduled Operation Setting Screen....................................................................1-41
Setting the Operation Ready/Shutdown Times.............................................................................1-42
Setting Days Off ...........................................................................................................................1-43
Deleting Days Off Setting .............................................................................................................1-43
Setting an Operating Day.............................................................................................................1-44
Deleting an Operating Day Setting...............................................................................................1-44
Using External Save.....................................................................................................................1-45
Backing Up to the Instrument.......................................................................................................1-45
Restoring from the Backup in the Instrument...............................................................................1-45
Outputting (Exporting) to a USB Memory Device .........................................................................1-45
Inputting (Importing) from a USB Memory Device........................................................................1-45
Selecting the Language.....................................................................................................................1-46
Displaying the Maintenance Information............................................................................................1-46
Setting the Cooling Method................................................................................................................1-46
Setting Sounds...................................................................................................................................1-47
Changing the Sound Volume........................................................................................................1-47
Changing the Sound.....................................................................................................................1-47
Setting Accounts ................................................................................................................................1-48
Switching between the Manager List and User List......................................................................1-48
Setting/Changing a Password......................................................................................................1-48
Various Permission Table .............................................................................................................1-49
Using External Save.....................................................................................................................1-50
Backing Up to the Instrument.......................................................................................................1-50
Restoring from the Backup in the Instrument...............................................................................1-50
Outputting (Exporting) to a USB Memory Device .........................................................................1-50
Inputting (Importing) from a USB Memory Device........................................................................1-50
Viewing and Outputting the History....................................................................................................1-51
Checking and Outputting the Error Log........................................................................................1-51
Checking and Outputting the Event Log.......................................................................................1-51

c
Software version ................................................................................................................................1-52
Checking the Version....................................................................................................................1-52
Updating.......................................................................................................................................1-52
Chapter 2 Operating Method
Basic Operation Flow ................................................................................... 2-1
Logging On to the System ........................................................................... 2-2
Performing Pre-operation Checks........................................................................................................2-2
Turning On the Power..........................................................................................................................2-2
Logging On to the Instrument...............................................................................................................2-3
Logging Off from the Instrument...........................................................................................................2-3
Returning from the Sleep Mode ...........................................................................................................2-3
Returning from the Standby Mode........................................................................................................2-3
Preparing for Sectioning .............................................................................. 2-4
Checking the Temperature Settings.....................................................................................................2-4
Checking the "FREE" Temperature Settings........................................................................................2-4
Changing the Chamber Temperature .............................................................................................2-4
Changing the Specimen Head Temperature...................................................................................2-4
Using "Temperature Setting 1" or "Temperature Setting 2"..................................................................2-4
Preparing for Frozen Block Preparation...............................................................................................2-5
Freezing in the Chamber......................................................................................................................2-5
Setting the Frozen Block on the Specimen Head.................................................................................2-6
Adjusting the Specimen Head..............................................................................................................2-7
Adjusting the Specimen Head Orientation (Angle)..........................................................................2-7
Changing the Disposable Blade...........................................................................................................2-8
Using the High Profile Type............................................................................................................2-8
Adjusting the Lateral Movement of the Blade Holder .....................................................................2-9
Using the Low Profile Type...........................................................................................................2-10
How to Set the Low Profile Adapter..............................................................................................2-10
Sectioning.....................................................................................................2-11
Preparing for Manual Sectioning (Final Shaving)...............................................................................2-11
Press the home position key to move the specimen head to the sectioning start position...........2-11
Cutting the Specimen to Flat Surface (Rough Shaving) ...............................................................2-11
Checking the Section Thickness for Sectioning (Final Shaving)................................................2-11
Manually Sectioning the Specimen (Final Shaving) ...........................................................................2-12
Sectioning Manually...........................................................................................................................2-12
Sectioning with theAnti-Roll Rake .....................................................................................................2-13
Sectioning with theAnti-Roll Plate......................................................................................................2-14
After Processing ......................................................................................... 2-15
Clean the Interior of the Chamber Using the Supplied Nozzle...........................................................2-15
Performing Cleaning Operation..........................................................................................................2-16
Cancelling Cleaning Operation.....................................................................................................2-17
Defrosting Manually ...........................................................................................................................2-18
Cancelling Manual Defrost Cycle .................................................................................................2-18
Performing Ozone Cycle Manually.....................................................................................................2-19
Cancelling Manual Ozone Cycle ..................................................................................................2-19
Shutting Down the Instrument............................................................................................................2-20
Shutting Down the Instrument Manually.............................................................................................2-20
Manual shutdown when "Always-on operation" is selected..........................................................2-20
Manual Shutdown under "Scheduled Operation"..........................................................................2-21
Cancelling Manual Shutdown.......................................................................................................2-22
Shutting Down the InstrumentAutomatically......................................................................................2-23
Cancelling Automatic Shutdown...................................................................................................2-24
Other Operations ........................................................................................ 2-25
When the Instrument Detects an Abnormality During the Ozone Disinfection Cycle..........................2-25
Errors notified after completion of ozone decomposition..............................................................2-25
Errors notified when ozone might still be inside the chamber.......................................................2-25

d
Preparing for Motorized Sectioning (DM only) ...................................................................................2-26
Preparing for Sectioning...............................................................................................................2-26
Selecting an Motorized Sectioning Mode .....................................................................................2-26
Moving the Specimen Head to the Sectioning Start Position........................................................2-27
Cutting the Specimen to Flat Surface (Rough Shaving) in the TRIM Mode..................................2-27
Checking the Section Thickness...................................................................................................2-27
Sectioning Automatically (DM only)....................................................................................................2-28
How to Operate the Right Panel...................................................................................................2-28
How to Operate the Foot Switch...................................................................................................2-28
Adjusting the Anti-Roll Plate...............................................................................................................2-29
Adjusting the Forward/Backward Position of the Anti-Roll Plate...................................................2-29
Adjusting the Angle (Lateral Inclination) of the Anti-Roll Plate......................................................2-29
Adjusting the Blade Holder.................................................................................................................2-30
Adjusting the Blade Angle (Relief Angle)......................................................................................2-31
Removing the Blade Holder from the Chamber............................................................................2-32
Setting the Blade Holder in the Chamber (Normal)......................................................................2-32
Setting the Blade Holder in the Chamber (Cryo3Adapter Is Used)..............................................2-33
Turning on the Chamber LED Lights..................................................................................................2-34
Assisting with Vacuum .......................................................................................................................2-34
Draft Generator..................................................................................................................................2-35
Starting the Draft Generator .........................................................................................................2-35
Stopping the Draft Generator........................................................................................................2-35
Changing the Suction Force of the Draft Generator.....................................................................2-35
Setting the Parking Plate..................................................................................................................2-36
Setting the Heat Extractor..................................................................................................................2-36
Plastic Object Holder..........................................................................................................................2-37
Using the Plastic Object Holder....................................................................................................2-37
Adapter for Cryo3 Object Holder........................................................................................................2-38
Using Adapter for Cryo3 Object Holder ........................................................................................2-38
Freeze the Block Using the PINO Hyfluid Method .............................................................................2-38
Chapter 3 Maintenance/Inspection
Daily Care and Inspection............................................................................ 3-1
Cleaning the Chamber.........................................................................................................................3-1
Cleaning the Chamber....................................................................................................................3-1
Cleaning the Chamber Drain Pipe..................................................................................................3-1
Cleaning the Specimen Head...............................................................................................................3-1
Cleaning the Holder Installation Position........................................................................................3-1
Cleaning the Operation Panel and Instrument Exterior........................................................................3-1
Setting the Waste Tank ........................................................................................................................3-2
Cleaning the Waste Tank .....................................................................................................................3-2
How to Install the Vacuum Filter Set ....................................................................................................3-3
Replacing the Vacuum Filter Set..........................................................................................................3-4
Cleaning the Vacuum Hose............................................................................................................3-4
How to Set the Draft Generator............................................................................................................3-5
Draft Generator Error Codes................................................................................................................3-5
Replacing the Draft Generator Filter ....................................................................................................3-6
How to Disassemble/Set and Clean the Blade Holder.........................................................................3-7
Disassembling/Cleaning the Blade Holder.....................................................................................3-7
Disassembling/Cleaning the Stage.................................................................................................3-8
Disassembling/Cleaning the Wave Washer and Lever Locking Bracket of the Blade Holder (Base)
.......................................................................................................................................................3-8
How to Disassemble/Set and Clean the Anti-Roll Plate .......................................................................3-9
How to Remove theAnti-Roll Plate and Vacuum Suction Port.......................................................3-9
How to Remove Only the Anti-Roll Plate........................................................................................3-9
How to Install the Anti-Roll Plate ....................................................................................................3-9
Disassembling and Assembling the Anti-Roll Plate ......................................................................3-10
Replacing the Glass of the Anti-Roll Plate....................................................................................3-10

e
Cleaning the Vacuum Suction Port...............................................................................................3-10
Cleaning the Anti-Roll Rake...............................................................................................................3-11
Disassembling/Cleaning the Anti-Roll Rake .................................................................................3-11
Cleaning the Object Holder................................................................................................................3-11
Cleaning the Window Gasket.............................................................................................................3-11
Replacing the Ozone Lamp................................................................................................................3-11
Cleaning the Air Intake Grille..............................................................................................................3-12
Replacement of the Brine Solution (auxiliary cooling fluid)................................................................3-12
Chapter 4 Troubleshooting
Troubleshooting............................................................................................ 4-1
Before Suspecting a Trouble ~ Instrument Conditions and Remedial Actions .....................................4-1
When Error/Alarm/Other Message Screens Appear ............................................................................4-4
Error Codes Displayed during System Operation ................................................................................4-5
Glossary of Terms......................................................................................... 4-7
Explanation of Terms............................................................................................................................4-7
Decontamination Statement
Warranty Period and Repair
Warranty Period and Repair

f
Safety Precautions
Please appoint the "person responsible for handling the instrument."
•The Polar® instrument requires special knowledge on the use and operation. Therefore, to use the
instrument safely, please appoint the "person responsible for handling the instrument."
•The "person responsible for handling the instrument" must directly be given handling instructions from the
sales staff in charge at the time of instrument delivery.
* Please keep this Operating Manual with the Polar® instrument for easy reference.
* Before using the instrument, read "Safety Precautions" to ensure the correct use.
* The precautions provided herein are intended to make sure the instrument is used safely in order to
protect the user, bystanders and environment from potential dangers and/or losses. They provide important
safety information and must be followed without fail.
* This document uses " " " " " " and " " to indicate varying degrees and levels of
imminence of danger and/or loss resulting from wrong handling. Their definitions are presented below.
Instructions provided under these headings are very important and must be followed without fail.
Wrong handling against the instruction may potentially result in the operator's death or serious injury.
Failure to follow the instruction may potentially result in the operator or other person suffering serious
injury.
Failure to follow the instruction may potentially damage to the Polar® instrument or other property or
negatively affect the processing result.
Point to note or other useful information.
* Specifications and other details of the Polar® instrument are subject to change without notice for the
reason of functional improvement, etc.
* Images featured in this document may differ from the actual product.
* Unauthorized reproduction of all or part of this document is strictly prohibited.
* We shall assume no responsibility whatsoever for any impact the frozen sections prepared by the Polar®
instrument may have on diagnosis, etc., or use of the Polar® instrument may have on tissues and
specimens.
•Symbols on the Polar® instrument labels attached to the instrument are explained.
Labels bearing the symbols below provide particularly important instructions that must be heeded to ensure
safety of the operator, provide greater ease of operation and protect the instrument from damage. Be sure
to check and follow the instructions as you commence your work.
Labels bearing this symbol provide compulsory actions. Be sure to follow the instructions.
Labels bearing this symbol provide prohibited items. Be sure to follow the instructions.
Labels bearing this symbol provide precautions. Wrong handling against the instruction may
potentially expose the operator to danger or damage the instrument, so be sure to follow the
instruction.
Labels bearing this symbol indicate that areas around them are heated to high temperatures.
Exercise caution against burns caused by high temperatures.
When handling any bio-hazard material on the Polar® instrument, follow the applicable
regulations and guidelines in the country or region to ensure safety.

g
Hole in the side
DANGER
•
Do not use power cords other than the one supplied with the products.
If a power cord with unsuitable current capacity is used, electric shock or fire due to short-circuit can occur.
Be sure to use a correct power cord that is suitable for the current capacity of your region.
•
Be sure to ground the instrument.
Be sure to ground the instrument to prevent electric shock and damage.
•
Do not disassemble, repair or modify the instrument.
Disassembling, repair and modification of the Polar® instrument must not be done by any other persons
except by our repair staff. If such work is done by any unauthorized person, explosion, electric shock,
equipment failure or injury can occur. These harm and damages may cause the instrument to malfunction
and result in accident.
•
Do not overload the outlet and do not use an extension cord.
Doing so may stop the operation of the instrument due to voltage drop, or fire can occur due to heat
generation.
•
Do not operate the switches with a wet hand.
Do not connect/remove the power plug with a wet hand.
Connect/remove the power plug by holding the plug.
Electric shock accident can occur.
•
Do not use the instrument with incorrect voltage.
Doing so may cause the instrument to overheat, cause electric shock, short-circuit, or cause unexpected
fire, etc. Before operating the instrument, be sure to check the voltage in use. The Polar® instrument has a
voltage switch, so set a proper voltage according to the region.
•
Do not forcibly bend, pull or twist the power cord or loop it into a bundle, as it may
damage the power cord.
Fire or electric shock accident can occur. Furthermore, do not place heavy objects on the power cord and
store it in a narrow place. Doing so may damage the power cord.
•
Do not place the instrument in a location exposed to direct sunlight, snow or rain.
Ultraviolet rays and high temperature may damage the instrument. Furthermore, using the instrument while
exposing it to snow or rain may cause electric shock, short-circuit, fire or malfunction.
•
Do not let foreign matters go inside the instrument.
If water, metal, paper or other object clogs the exhaust port (heat
radiating port), fire, electric shock, injury of the user or instrument
failure may occur. If foreign matters entered the instrument, stop the
instrument as soon as possible and contact the nearest authorized
Sakura distributor or representative.
Also, the hole in the side is exclusively for only our service staff to
operate. Please do not touch this hole.
•
Do not put your face into the chamber when working.
Doing so may cause a risk of infection because infectious samples may be sometimes handled in the
chamber from the nature of this system.
•
Do not open the window during the ozone disinfection cycle.
Ozone might leak from the instrument.
[Precautions When Performing Ozone Disinfection Cycle]
High concentration ozone gas is generated in the chamber during the ozone disinfection cycle. High
concentration ozone gas is extremely dangerous. Do not open the window until ozone decomposition
treatment is completed.
* Ozone is a colorless gas with a pungent odor.
In the event that you feel irritation as a result of the ozone exposure, perform the following proper treatment.
•If you feel irritation in your eyes: Rinse with water.
•If you inhale ozone: Immediately move to somewhere else where the air is clean.
•If you feel irritation on your skin: Rinse with water.
•In the case of serious injury or if symptoms do not improve: Seek medical help from a doctor.

h
Safety Precautions
WARNING
•
Securely connect the power cord.
If they are loosely connected, heat may be generated at the connected part, voltage drop can occur and the
instrument may not operate normally. Furthermore, unexpected accidents or injuries can occur.
•
Before using the instrument, be sure to always check for the use of a correct power cord
and specified voltage.
If an incorrect power cord is used, fire, instrument malfunction due to voltage drop, unexpected accidents or
injuries can occur.
•
Do not store or use the instrument in a humid or dusty place.
Using the instrument in a humid or dusty place may cause its parts to deteriorate, short-circuit, fire, etc. The
installation humidity range is 85% or below (non-condensing).
•
Thoroughly check the installation location before installing the instrument.
Install the Polar® instrument on a flat, non-slippery sturdy floor or bench free from vibration and having a
sufficient size. If the installation location is tilted or too small, unexpected accidents may occur.
•
Turn off the power before cleaning the instrument.
Unless otherwise instructed in this document, always turn off the power before cleaning the instrument.
•
Install the instrument in a well-ventilated cool place.
Do not block the exhaust vent (radiation vent) with a wall or object or use the instrument in a poorly
ventilated place or with the dustproof covers still attached, because heat will be trapped in the instrument
and cause abnormal overheating, potentially causing fire, accident, failure, etc.
•
Do not directly touch with hands, etc. those areas whose temperature drops to low
levels.
Never directly touch with hands, etc. the chamber and other locations subject to extremely low
temperatures. Frostbite may occur. When touching the chamber, wear gloves or use dry, thick cloths. It is
recommended to wear thick rubber gloves for safety.
•
Be sure to lock the handwheel and various levers and put the blade guard on when the
instrument is not in use.
A disposable blade is set in the blade holder. When setting a tissue sample, unexpected movement of the
specimen head (specimen holder) or blade holder may cause injury. Even when the instrument is not in use,
be sure to lock the handwheel and various levers and put the blade guard on. Also, always wear protective
gears when using the instrument.
•
Do not open the window during the ozone disinfection cycle.
Ozone gas generates in the chamber during the ozone cycle. Ozone is potentially harmful to the human
body, so do not open the window until ozone decomposition treatment is completed.
•
Turn off the power should malfunction occur.
If the motor malfunctions or generates abnormal noise or the ozone lamp turns on erroneously, immediately
turn off the power and contact the nearest authorized Sakura distributor or representative.
Install the instrument in such a way that operation of the power switch will not be obstructed.
•
Use a cooling spray containing HFC-134a gas.
Do not use a cooling spray containing butane or propane. Use of such sprays may cause instrument
malfunction. When a cooling spray is used, use a cooling spray containing HFC-134a
(1,1,1,2-Tetrafluoroethane) as a main component.

i
WARNING
•
When disinfecting the instrument or chamber, do so in a well-ventilated place wearing
protective gears. Also use the specified disinfectant/cleaning reagent.
Using any other reagent may cause the instrument to corrode, fail, etc. For details, refer to Chapter 3,
"Maintenance/Inspection."
•
After use, clean/disinfect the instrument as soon as possible.
Debris and unwanted trimmings, etc., accumulate in the chamber during sectioning. These sectioned craps
are potentially infectious, so clean/disinfect the chamber as soon as possible.
If alcohol, acetone or other highly volatile reagent was used for cleaning/disinfection, dry the chamber
thoroughly. Otherwise, the instrument may fail or the interior walls and/or components such as the
evaporator in the chamber may corrode.
•
Replace the vacuum filter set regularly.
The pre-filter and bacterial filter (vacuum filter set) are consumables. Replace them every 3 months
depending on the condition of the institution.
•
Clean the waste tank regularly.
The Polar® instrument automatically defrosts itself once a day. Liquid generating in the chamber as a result
of defrost cycle collects in the waste tank, and since this liquid waste is potentially infectious, drain and
clean the waste tank regularly. Drain the waste tank in a well-ventilated place and wear protective gears.
If alcohol, acetone or other highly volatile reagent was used for cleaning/disinfection, dry the chamber
thoroughly. Otherwise, the instrument may fail or the interior walls and/or components such as the
evaporator in the chamber may corrode.
•
Wear a mask, gloves, goggles and other protective gears while working or cleaning.
The Polar® instrument has a blade and glass edges to demonstrate its characteristics. Since these parts
may cause injury or infection, be sure to wear gloves and other protective gears while working (sectioning,
changing the disposable blade, cleaning, etc.).
•
Do not perform the ozone disinfection cycle in a high altitude place.
If the ozone cycle is performed at a high altitude place of 2000 m or above, the ozone cycle may not be
implemented properly and infection may occur during work. Be sure to install the instrument in a location
meeting the installation conditions.
•
Keep the chamber, blade holder and specimen head (specimen holder) against
attachment of contaminants at all times.
If contaminants are attached to the blade holder or specimen head (specimen holder), tissues may not be
sectioned to an appropriate thickness or irregular thickness or tissue sample contamination may occur. Also
keep the chamber from attachment of contaminants at all time, because if sectioned craps accumulate in
the chamber, liquid waste may not be drained properly during defrost cycle.
•
Make sure the Anti-Roll Rake does not come in direct contact with the tissue sample.
The Anti-Roll Rake leaves edge marks on the tissue sample. So that proper specimens can be prepared,
make sure no tissue sample is present in areas that may come in contact with the Anti-Roll Rake.
•
Handle the Anti-Roll Plate made of glass with care.
If the Anti-Roll Plate falls sharply, its front end may get contact with a disposable blade and broken. This
may lead to scatter of glass fragment and cause injury. The brokenAnti-Roll Plate may also interfere with
production of good sections or cause damage to blocks.

j
Safety Precautions
WARNING
•
Do not touch the switches (especially the motorized sectioning switch) unless
necessary.
Pressing the motorized sectioning switch causes the specimen head (specimen holder) to move up/down. If
the specimen head (specimen holder) is not at an appropriate position, the specimen may contact the blade
holder, resulting in instrument failure or tissue sample damage. If the specimen head (specimen holder)
was moved by mistake, immediately stop the instrument by pressing the emergency stop switch.
•
Provide a space of at least 20 cm around the instrument.
If sufficient space is not available around the exhaust instrument, heat will be trapped and the cooling
function will drop. Be sure to install the instrument in a location meeting the installation conditions.
•
Do not install the instrument in a hot, humid place or place subject to the direct sunlight.
If the instrument is installed in a hot/humid place or place subject to the direct sunlight, the chamber and
blade holder may not be cooled properly. Be sure to install the instrument in a location meeting the
installation conditions.
•
Install the power cord carefully to make sure it will not obstruct work.
The operator may trip over the power cord and fall or get injured.
•
Do not leave the instrument with the tissue sample/disposable blade still in the chamber.
The Polar® instrument automatically defrosts itself once a day. The chamber temperature rises to 0°C or
above during the defrost cycle (even in the event of power outage), so the tissue sample may melt. Also,
the disposable blade may frost and rust. Do not leave the tissue sample and disposable blade in the
chamber.
•
Set the chamber temperature and specimen head (specimen holder) temperature (D/DM)
to levels appropriate for the tissue/compound beforehand.
If the chamber and specimen head (specimen holder) temperature settings are too high, the tissue sample
and compound may melt. Be sure to set temperatures matching the tissue sample and compound used,
prior to sectioning.
•
Inspect the system every 6 months.
Contact the nearest authorized Sakura distributor or representative for the details of periodic inspection.
•
Be sure to lock the main body with the adjuster.
When the handwheel is turned, the instrument may vibrate and cause unexpected accident. Lock the main
body with the adjuster.
•
Clean the Object Holder after use.
If tissue/compound deposits remain on the Object Holder, contamination may occur or tissues may not be
held by the Object Holder properly and separate. Be sure to clean the Object Holder after use.
•
When handling a disinfectant such as glutaraldehyde, wear gloves, mask and/or safety
goggles, or take other proper measures designed to protect the operator, by following
applicable regulations or guidelines in your country or region. Dispose of wastes properly
as per regulations and guidelines in your country or region. Remove disinfectant residue
thoroughly after use.
Some disinfectants are a hazard to human health.
Disinfectant residue may cause the instrument to corrode or malfunction.
If any biologically hazardous substance is handled, follow applicable regulations or guidelines in your
country or region to ensure safety.

k
WARNING
•If there is a power interruption during the ozone disinfection cycle, do not open the
window.
Ozone might leak from the instrument.
[Refer to page (g) "Precaution When Performing Ozone Disinfection Cycle"]
* Ozone disinfection involves the generation of high concentration ozone inside a sealed chamber under
negative pressure with a locked window to eliminate bacteria and viruses and/or fungi.Although the window
is locked even after a power interruption, the inside of the chamber is no longer in a negative pressure state.
For this reason, ozone might leak from the chamber if an attempt is made to open the window.
•
If there is the possibility of ozone still remaining in the chamber as a result of a power
interruption, move at least 30 cm away from the edge of the window on the instrument
until power is restored.
Ozone might leak from the instrument.
[Refer to page (g) "Precaution When Performing Ozone Disinfection Cycle"]
•
If an error occurs during the ozone disinfection cycle, operate by following the
on-screen messages. Also, if an error that cannot automatically be recovered occurs, do
not touch the window on the instrument, but immediately contact the nearest authorized
Sakura distributor or representative.
If an error that cannot automatically be recovered occurs, there might be ozone still inside the chamber.
Also, even if an error can automatically be recovered, to be extra sure contact the nearest authorized
Sakura distributor or representative before using the instrument.
•
Do not place or drop heavy objects on the window.
This might damage or crack the window, and cause ozone to leak from the instrument.
•
If the window is damaged or cracked, do not perform ozone disinfection.
If the window is damaged or cracked, the window might break during the ozone disinfection cycle.
Immediately contact the nearest authorized Sakura distributor or representative.
•
If the motor operates or sounds abnormally OR if the ozone lamp lights up when it is not
supposed to, OR if you notice other abnormalities, do not open the window but
immediately turn the power OFF.
There might be ozone still inside the chamber. Immediately contact the nearest authorized Sakura
distributor or representative.
[Refer to page (g) "Precaution When Performing Ozone Disinfection Cycle"]
•
During the ozone disinfection cycle, do not turn the handwheel.
Turning the handwheel during the ozone disinfection cycle might cause a rupture in the rubber seal that
separates the outer air from inside the chamber and cause ozone to leak.
[Refer to page (g) "Precaution When Performing Ozone Disinfection Cycle"]

l
Safety Precautions
CAUTION
•
Unplug the power cord from the power outlet and store it while the instrument is not in
use.
For safety, if the instrument will not be used for an extended period of time, roughly clean the entire
instrument, cover it to prevent dust and foreign matters from entering, and store it in a place with least
humidity.
•
Handle the power cord with care.
To unplug the power cord from the power outlet, do not pull the cord, but hold the plug and then pull it out.
Furthermore, inspect the power cord periodically. If any damage is found in the exterior of the power cord,
stop using it and contact the nearest authorized Sakura distributor or representative.
•
Be sure to inspect the instrument periodically.
Maintain/inspect the instrument periodically so that it operates correctly at all times.
Attachment of condensation water, frost, ice or contaminant on the top face of the Cryo Plus and Cryobar or
dirt or dust on the intake port cover may cause the cooling capacity to drop. Also, the ozone disinfection
cycle may be disabled if the window gasket is damaged or has deposits of foreign matters. Inspect the
window gasket periodically according to "Daily Care and Inspection."
•
Pay attention to the reagent to be used.
If alcohol, acetone or other highly volatile reagent or dry ice was used, dry the chamber thoroughly.
Otherwise, the instrument may fail or the interior walls and/or components such as the evaporator in the
chamber may corrode.
•
Keep the window closed while the instrument is not in use.
If the instrument will not be used for an extended period of time, be sure to close the window. Otherwise, the
cooling part in the chamber may frost and cause the operability or cooling performance to drop.
•
Do not put reagent, etc., on the instrument.
If reagent spills over the instrument, the instrument may fail or sustain damage.
•
Do not leave a non-sealed container containing a volatile reagent such as alcohol,
acetone and formalin inside the chamber.
Doing so may cause the interior walls and/or components such as the evaporator in the chamber to corrode
because such reagents will be oxidized with ozone during the ozone disinfection cycle and produce formic
acid or acetic acid.
•
If an abnormality occurs during the ozone disinfection cycle, perform ozone disinfection
again.
If an abnormality occurs during the ozone disinfection cycle, ozone disinfection (generation and
decomposition of ozone) might not have been performed appropriately. For this reason, when an infectious
specimen has been handled, the source of the infection might not have been eliminated. Repeat ozone
disinfection by manual operation. If an error recurs, contact the nearest authorized Sakura distributor or
representative.
•
Do not use tools or foreign objects to force open or to access the interior of the
instrument.
If water, metal, paper or other foreign objects get inside the exhaust port (heat radiating port), or other holes
or gaps on the exterior, fire, electric shock, injury to the user, or instrument failure may occur. If foreign
objects get inside the instrument, immediately stop the instrument, and contact the nearest authorized
Sakura distributor or representative.

Chapter 1 Basic Knowledge of Instrument

1-1
Basic Knowledge
Explanation of System
The Tissue-Tek® Polar® is an instrument to freeze and section tissues and prepare sections.
•
Preparation of high-quality frozen specimens (D&DM type: Standard specification)
Proper cooling of the specimen head (specimen holder) and area around the blade holder in the chamber
allows for preparation of high-quality frozen tissue sections. In particular, the specimen head (specimen
holder) reaches the setpoint temperature in just several seconds, so all types of tissues from lymph node
tissues to fatty tissues can be sectioned rapidly in an ideal temperature band.
•
Very easy-to-use operation panel and chamber design
The home position key *1, trim key, etc., let you section tissues in fewer steps. The wide chamber also
allows for smooth collection of sections. Interior walls of the chamber are flat for easy cleaning.
*1: The home position key lets you move the specimen head to the registered position to start cutting with a
touch of the key.
•
Prevention of infection after sectioning (D&DM type: Standard specification)
Ozone disinfection and debris removal functions are provided for bio-hazard control.
The process completes in approximately 75 minutes under cooling to reduce the risk of bacterial or viral
infection in the chamber.
•
Prevention of infection during sectioning (D&DM type: Optional)
To prevent infection during sectioning, a draft generator can be installed to keep contaminated air from
diffusing outside the chamber. This function prevents infection during sectioning.

1-2
Installation Method
This section provides information on determining a location for, and installing the Polar® instrument.
Installation should be performed by qualified service staff working exclusively with the Polar® instrument.
The Polar® instrument must be installed correctly to ensure proper operation and service.
Read this Operating Manual carefully before operating the Polar® instrument. Follow all instructions
provided in this document carefully.
The Polar® instrument is a precision instrument and must be handled accordingly. Rough handling or
dropping will disturb or damage internal components. Always handle the Polar® instrument with care.
Environmental Factors
As with all electronic equipment, the Polar® instrument must also be installed and used in an appropriate
environment (temperature, humidity, etc.).
Temperature and humidity should be held constant to obtain the highest degree of operating stability. The
ambient operating temperature range of the Polar® instrument is 10 to 35°C. The ambient operating
humidity range is 30 to 85% relative humidity, non-condensing. Locate the instrument in a well-ventilated
area at least 20 cm away from walls, avoiding exposure to corrosive vapors, direct air currents, or
temperature extremes. Avoid proximity to direct sunlight, open windows, sinks, ovens, open flames, hot
plates, radiators, and dry ice baths. Locate the Polar® instrument away from any equipment that consumes
a high voltage or large current, including large refrigerators and ovens. Since the instrument is very heavy,
the floor must be solid and level, and the instrument should be secured with adjusters (lockdown feet).Also
install anchorage brackets, etc., in case of earthquake.
Be sure the instrument is located near a power source that meets the electrical requirements (voltage and
amperage) specified in the specification page of this Operating Manual. The power receptacle must be
grounded and should be a clean, dedicated line.
Using the Polar® instrument in an unventilated location may do serious harm to the human body.

1-3
Name of Each Part (Exterior)
[1] Window and chamber
The window is designed with an anti-fogging feature. To open the window, lift the handle and push it
away from you. Refer to p. 1-6 for the details of the chamber.
[2] Left panel
This panel is used to operate the vacuum function, move the specimen head (specimen holder) forward
and backward, and operate the trim function. Refer to p. 1-5 for details.
[3] Status lamps and display
This touch panel is used to perform various operations and settings. Refer to p. 1-5 for the status lamps.
[4] Emergency stop switch
This switch is used to stop the microtome operation. Refer to p. 1-13 for details.
[5] Right panel (DM only)
Motorized sectioning can be started or stopped and the sectioning speed adjusted on this panel, among
others. Refer to p. 1-5 for details.
[6] USB port, LAN port, foot switch port (DM only)
Refer to p. 1-4 for details.
[7] Handwheel lock tab
This lever is used to lock the handwheel. Refer to p. 1-8 for details.
[8] Handwheel with handle
This handwheel is used during sectioning. Refer to p. 1-7 for details.
[9] Main power switch
[10] Waste tank
Liquid waste is collected here during the defrost cycle. Refer to p. 3-2 for the setting method.
[11] Foot rest
This foot rest is used during sessions.
[12] Adjusters (lockdown feet)
These adjusters are used to secure the system.

1-4
[13] Draft generator (optional)
This optional draft generator prevents contaminated air from diffusing outside the chamber. Refer to p.
2-34 for details.
[14] Filtering power switch for draft generator
This switch is used to supply power to the filtering mechanism of the optional draft generator. Refer to p.
2-34 for details.
[15] Filtering suction force switching button for draft generator
The suction force of the optional draft generator can be set to one of five levels. The air volume can be
checked with the center indicator lamp. Refer to p. 2-34 for details.
[16] Draft generator filter LEDs
The two LEDs tell you when to replace the HEPA filter in the optional draft generator and when the draft
generator is operating abnormally (such as when the fan has stopped).
[17] USB port
A USB memory device can be connected here. Use this port to export data, etc.
[18] LAN port
A LAN cable can be connected here.
[19] Foot switch port (DM only)
This port is used to connect the foot switch. Once the foot switch is connected, motorized sectioning can
be started, paused or stopped using the foot switch. Refer to p. 1-9 for details.
[20] Power cable

1-5
Name of Each Part (Left/Right Panels, Display Area)
[1] Vacuum key
This switch is used to suction air form the vacuum inlet. Pressing this key once applies 100% suction
force, while pressing it again adjusts the suction force to a desired level according to the setting. Refer
to p. 1-36 for details.
[2] Home position key
This key is used to move the specimen head (specimen holder) to the home position (set position to
start cutting). Refer to p. 1-34 and p. 2-11 for details.
[3] R key
Press this key to back away the specimen head (specimen holder).
The specimen head backs away by 10 µm every time this key is pressed. Press and hold the key, and
the specimen head continues to back away while the key is held.
[4] F key
Press this key to advance the specimen head (specimen holder).
The specimen head advances by 10 µm every time this key is pressed. Press and hold the key, and the
specimen head continues to advance while the key is held.
[5] TRIM key
Turn the handwheel while holding this key to section the tissue to flat surface at a desired trim thickness
according to the setting. Refer to p. 1-31 for details.
[6] Speed adjustment lever (DM only)
The sectioning speed can be adjusted with this lever during motorized sectioning. Raise the lever to
increase the speed, or lower it to decrease the speed.
[7] Motorized sectioning key (DM only)
This key is used to start, pause or stop motorized sectioning. Press the pause key (  ) and stop key ()
together to start motorized sectioning. Refer to p. 1-11 for details.
[8] Power indicator lamp
This lamp turns on when the power is supplied.
[9] System status indicator lamp
This lamp is lit when the system is ready to perform sectioning and other operations.
It blinks if sectioning operation is not possible because the ozone cycle, etc., is in progress.
[10] Sleep status indicator lamp
This lamp turns on when the system sleeps following a manual/automatic shutdown. It turns off once the
system wakes up.