SCIEX ExionLC 100 Installation manual

ExionLC™100 System
Hardware User Guide
May 2018RUO-IDV-05-1839-C

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
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©2018 AB Sciex
AB Sciex Pte. Ltd.
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Woodlands Central Indus. Estate.
SINGAPORE 739256
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1 Operational Precautions and Limitations...............................................................................................6
Electrical Precautions.........................................................................................................................................................6
AC Mains Supply..........................................................................................................................................................6
Protective Earth Conductor..........................................................................................................................................7
Environmental Precautions................................................................................................................................................7
Electromagnetic Environment......................................................................................................................................8
Decommissioning and Disposal....................................................................................................................................8
Ventilation Precautions......................................................................................................................................................9
Chemical Precautions.........................................................................................................................................................9
System Safe Fluids.....................................................................................................................................................10
Static Electricity Precautions ...........................................................................................................................................11
Typical Cause of Static Electricity Accidents...............................................................................................................11
Preventing Static Electricity Accidents.......................................................................................................................14
Equipment Use and Modification.....................................................................................................................................16
Maintenance, Inspections, and Adjustment.....................................................................................................................17
Storage and Handling......................................................................................................................................................18
2 Overview ...............................................................................................................................................19
Front Side.........................................................................................................................................................................20
Side View.........................................................................................................................................................................22
Rear Panels......................................................................................................................................................................24
Interior View....................................................................................................................................................................25
Flow Line Diagram...........................................................................................................................................................27
Entire Flow Line.........................................................................................................................................................27
Inner Volume of Each Flow Line.................................................................................................................................28
Flow Line During Sample Injection Operations................................................................................................................29
Ready.........................................................................................................................................................................29
Release of Pressure from the Flow Line.....................................................................................................................31
Needle Movement.....................................................................................................................................................32
Needle Rinse Before Sample Aspiration.....................................................................................................................33
Sample Aspiration......................................................................................................................................................35
Needle Rinse After Sample Aspiration.......................................................................................................................36
Start Analysis.............................................................................................................................................................37
Initialize the Measuring Pump...................................................................................................................................39
Rinse Solution Aspiration...........................................................................................................................................40
Rinse Solution Discharge............................................................................................................................................41
3 Configuration ........................................................................................................................................43
Configure Initial Settings.................................................................................................................................................43
Show the Calibration Tab...........................................................................................................................................43
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Contents

Set a Safety Lock........................................................................................................................................................52
Set Password Request to Deactivate the Lock............................................................................................................54
Configure Network Settings.......................................................................................................................................57
Set the Date and Time...............................................................................................................................................61
Change the System Pass ID........................................................................................................................................64
Change the Login Pass ID..........................................................................................................................................66
Initialize Settings........................................................................................................................................................69
Configure the System to Shut Down after Analysis....................................................................................................71
4 Operating Instructions...........................................................................................................................75
Sample Workflow.............................................................................................................................................................75
Turn on the HPLC System.................................................................................................................................................76
Log on to the HPLC System..............................................................................................................................................77
Prepare the Mobile Phase and Rinse Solution..................................................................................................................77
Select a Rinse Solution...............................................................................................................................................79
Install the Mobile Phase and Rinse Solution on the Reservoir Tray..................................................................................84
Perform an Autopurge.....................................................................................................................................................85
Install the Column............................................................................................................................................................90
Install the Column in the Column Oven.....................................................................................................................90
Equilibrate the Column..............................................................................................................................................95
Prepare the Sample..........................................................................................................................................................96
Put the Sample In a Sample Vial................................................................................................................................96
Put the Sample in the Autosampler...........................................................................................................................99
Removal of Sample Racks During Analysis....................................................................................................................101
Post-Analysis Procedures...............................................................................................................................................102
Rinse the Flow Line..................................................................................................................................................102
Turn Off the System.................................................................................................................................................102
5 Service and Maintenance....................................................................................................................104
Replace the Mobile Phase..............................................................................................................................................105
Replace the Mobile Phase with a Miscible Solvent..................................................................................................107
Replace the Rinse Liquid (if applicable)..........................................................................................................................111
Purge the Pump Manually..............................................................................................................................................115
Prime the Autosampler Rinse Solution Manually...........................................................................................................119
Change Sample Racks....................................................................................................................................................124
Set the Rack ID.........................................................................................................................................................124
Install the Plates on the Sample Rack......................................................................................................................130
Open the Rack Setting Screen..................................................................................................................................134
Set the Plate Type and Needle Stroke from the Top.................................................................................................138
Adjust the Rack Position..........................................................................................................................................142
Connect the System to a Computer................................................................................................................................159
Determine the Amount of Mobile Phase and Rinse Solution Remaining........................................................................160
Lock the System.............................................................................................................................................................162
Clean the Leak Sensor and Leak Tray.............................................................................................................................165
Clean the Automatic Rinsing Kit (if applicable)..............................................................................................................167
LC Column.....................................................................................................................................................................167
Clean the Monitor Screen..............................................................................................................................................168
Clean the Module Surfaces............................................................................................................................................170
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Contents

Storage and Handling....................................................................................................................................................171
6 Troubleshooting...................................................................................................................................172
Hardware Issues.............................................................................................................................................................172
Analysis Issues...............................................................................................................................................................177
Causes of and Countermeasures for Clogging in Flow Lines..........................................................................................182
A Consumables, Options, and Spares....................................................................................................184
Consumables.................................................................................................................................................................184
Sample Vials for Use with the Autosampler.............................................................................................................185
Septum for Use with the Autosampler.....................................................................................................................188
Microtiter Plate........................................................................................................................................................189
Pump Spares..................................................................................................................................................................191
Autosampler Spares.......................................................................................................................................................192
Column Oven Spares......................................................................................................................................................193
Options..........................................................................................................................................................................193
Other..............................................................................................................................................................................193
B Error Messages.....................................................................................................................................195
C Glossary of Symbols............................................................................................................................214
D Glossary of Warnings..........................................................................................................................219
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Contents

This guide describes the basic operation and troubleshooting for the ExionLCTM 100 system . Read this guide
thoroughly before using the product and operate the product in accordance with the instructions in this guide.
This guide provides safety instructions and precautions to make sure that the user operates the system safely.
Follow all Warning and Caution instructions provided in the guide.
Keep this guide for future reference. Make sure that it is accessible to the operator of the system.
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
•Follow required electrical safe work practices.
•Use cable management practices to control electrical cables. This will reduce the chance of a tripping hazard.
AC Mains Supply
Connect the system to a compatible AC mains supply as instructed in this guide.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the
installation of all of the electrical supplies and fixtures, and make sure that
all of the installations adhere to local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.
WARNING! Electrical Shock Hazard. Use only the power cables supplied with
the system. Do not use power cables that are not properly rated for the
operation of this system.
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1
Operational Precautions and
Limitations

CAUTION: Potential System Damage. Do not unpack or connect any system components.
The FSE will unpack, connect, and configure the system for the proper operating voltage.
Guidelines:
•Do not connect the wiring in a manner other than that prescribed by the manufacturer.
•Do not rest heavy objects on the power cable.
•Do not bend or pull on the power cable. To disconnect the system, pull on the plug and not the cable.
•Do not route the power cable near heat-generating equipment.
•Do not modify the power cable in any way.
Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the
protective earth conductor. Any interruption of the protective earth conductor
creates an electrical shock hazard.
Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all of the installations comply with local bylaws and biohazard regulations. For information
about the required environmental conditions for the system, refer to the Site Planning Guide.
Allow access space around the equipment when setting up the system.
WARNING! Fire Hazard. Do not operate the system in the presence of an open flame,
or in the same room as equipment that could potentially emit sparks.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part
is not intended to act as a biological containment.
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Operational Precautions and Limitations

WARNING! Environmental Hazard. Follow established procedures for disposal
of biohazardous, toxic, radioactive, and electronic waste. The customer is
responsible for disposal of hazardous substances, including chemical, waste
oils and electrical components, in accordance with local laws and regulations.
WARNING! Fire Hazard. Do not use flammable sprays (such as hair sprays or
insecticide sprays) near the system. They could ignite and cause a fire.
CAUTION: Potential System Damage. Avoid exposure to corrosive gas and excessive dust.
CAUTION: Potential System Damage. Take precautions to prevent the system from falling
in the event of an earthquake.
Electromagnetic Environment
CAUTION: Potential Wrong Result. Do not use this device in close proximity to sources of
strong electromagnetic (EMC) radiation (for example, unshielded intentional RF sources),
because EMC radiation might interfere with the proper operation and cause a wrong result.
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended.
Decommissioning and Disposal
Before decommissioning, decontaminate the entire system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations. Refer to Storage and Handling.
Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Waste Electrical and Electronic Equipment
Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of
waste, electrical, and electronic equipment (WEEE). To safely dispose of this equipment, contact a local Customer
Service office for complimentary equipment pick-up and recycling.
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Operational Precautions and Limitations

Ventilation Precautions
The venting of fumes and disposal of waste must comply with all of the federal, state, provincial, and local health
and safety regulations. It is the responsibility of the customer to make sure that the air quality is maintained in
compliance with local health and safety regulations.
WARNING! Flammable Chemical Hazard, Biohazard, Ionizing Radiation Hazard,
and Toxic Chemical Hazard. Be sure to use the system in a well-ventilated
laboratory environment in compliance with local regulations and with
appropriate air exchange for the work performed. Solvents used in high
performance liquid chromatography are flammable and toxic.
Chemical Precautions
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Determine whether decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with the system.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
WARNING! Fire Hazard. If large amounts of flammable waste solvents are collected,
then take preventative measures to minimize the risk of electrical fire due to static
electricity.
CAUTION: Potential System Damage. Do not submerge the end of the drain tubing in the
waste liquid in the waste container.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX
Safety Data Sheets can be found at sciex.com/tech-regulatory.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
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Operational Precautions and Limitations

•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Make sure that all exhaust hoses are connected properly and that all connections are functioning as designed.
•Collect all spent liquids and dispose of them as hazardous waste.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
•(Recommended) Use secondary containment trays beneath the solvent bottles and the waste collection container
to capture potential chemical spills.
System Safe Fluids
The following fluids can safely be used with the system.
CAUTION: Potential System Damage. Do not use any other fluid until confirmation is
received from SCIEX that it does not present a hazard. This is not an exhaustive list.
•Organic Solvents
•MS-grade acetonitrile; up to 100%
•MS-grade methanol; up to 100%
•Isopropanol; up to 100%
•HPLC-grade or higher water; up to 100%
•Buffers
•Ammonium acetate; less than 1%
•Ammonium formate; less than 1%
•Acids and Bases
•Formic acid; less than 1%
•Acetic acid; less than 1%
•Trifluoroacetic acid (TFA); less than 1%
•Heptafluorobutyric acid (HFBA); less than 1%
•Ammonia/ammonium hydroxide; less than 1%
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Operational Precautions and Limitations

Static Electricity Precautions
Liquid chromatography (LC) uses flammable organic solvents as the mobile phase. LC systems are also often used
where large amount of flammable substances are present. Thus, there is a risk of accidents involving fire or
explosion.
The major cause of these accidents is static electricity. Devising preventative measures for static electricity can be
difficult, because the symptoms before an accident vary and can be hard to detect, because such accidents occur
as a result of several simultaneous incidents. Recommended methods for preventing static electricity accidents
are provided in the following sections.
Typical Cause of Static Electricity Accidents
Static electricity accidents are generally caused by this sequence of events:
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Operational Precautions and Limitations

Table 1-1 Static Electricity Accidents—Sequence of Events
ResultEvent
When liquid is passed through thin tubing at high flow rates, as in liquid
chromatography, the electrostatic charges of the flowing matter generate static
electricity.
Figure 1-1 Generation of Static Electricity by a Liquid Flowing Over
a Solid
DescriptionItem
Flowing liquid1
Solid2
Charges move with the flow of liquid3
Immobile charges, fixed to the surface of the solid4
Generation of static activity.
If electrostatically charged liquid is allowed to accumulate in an electrically
insulated container, then the charge will gradually increase, and can eventually
reach several thousand volts.
Accumulation of static
electricity.
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Operational Precautions and Limitations

Table 1-1 Static Electricity Accidents—Sequence of Events (continued)
ResultEvent
If this happens and an electrical conductor is brought within a certain distance of
the container, then an electrical discharge occurs, releasing thermal energy that
will ignite any flammable gas of sufficient density in the vicinity.
Release of energy through
electrical discharge.
Figure 1-2 Potential Accident Situation
DescriptionItem
Dry air1
Spark2
Liquid flowing through thin tubing at a high rate. Air bubbles
in liquid facilitate generation of static electricity.
3
Insulated container of polyethylene or a similar material4
Flammable gas present in the container5
Flammable organic solvent with a large electrostatic charge6
Floor covered with rubber or a similar material cannot
conduct electricity away
7
Ignition of flammable
substances.
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Operational Precautions and Limitations

Preventing Static Electricity Accidents
The best way to prevent static electricity accidents is to prevent the occurrence and accumulation of electrostatic
charges. If large amounts of flammable solvent are collected in a large container, then the preventive measures
identified in Preventive Measure One, Preventive Measure Two, and Preventive Measure Three.
CAUTION: Potential System Damage. Take multiple preventative measures simultaneously.
Keep the room at a proper humidity level. Ambient humidity exceeding 65% will prevent
static.
Note: For low conductivity (less than 10–10 S/m) liquids, take preventive measures 1 to 4. For these liquids,
preventive measure 5 has no effect.
Note: Anti-static equipment (anti-static clothing, shoes, and matting) and charge measurement equipment
(potentiometer) are sold by specialty manufacturers.
Figure 1-3 Preventative Measures for Static
DescriptionItem
18 L metal can (preferably plated). (Preventive Measure One)1
Reduce the opening with a cap. (Preventive Measure Two)2
Connect a clip to metal parts.3
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Operational Precautions and Limitations

DescriptionItem
Connect to a protective earth terminal or other grounding point of the module. (Preventive
Measure One)
CAUTION: Potential System Damage. Do not connect the grounding
wire to the gas tubing, the water service tubing, or the telephone line.
4
Static electricity generated by the liquid will be conducted through the container to the
earth.
5
Preventive Measure One
Use a metal container for the waste liquid, and connect the container to protective earth to make sure that the
electrical charges of the container and liquid pass to the earth.
Required Materials (not provided)
•Grounding wire with clip
•18 L metal container
•4 L metal container
Guidelines
•Connect the metal waste container to protective earth properly. If the grounding wire is not properly connected
to protective earth, then static electricity can build up in the container.
CAUTION: Potential System Damage. Do not connect the grounding wire to the gas
tubing, the water service tubing, or the telephone line.
Note: Some metal containers have surfaces that are laminated or oxidized, and therefore do not conduct
electricity. After connecting the metal container to protective earth, use a tester to verify that electricity is
conducted to the earth.
•If the liquid to be drained into the waste container is virtually nonconductive (10–10 S/m or less), then add
properly conductive liquid to the tank. This conductive liquid can be added beforehand.
Preventive Measure Two
Cover the spaces between the tubing and the sides of the inlet and outlet openings of the waste container with
caps or other protective covering. This prevents any sparks generated outside the container from getting inside.
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Operational Precautions and Limitations

Required Materials (not provided)
•Caps for 18 L or 4 L container (with three 3 mm diameter openings)
Preventive Measure Three
Keep electrostatically charged objects, including the human body, away from the waste liquid container.
CAUTION: Potential System Damage. If no other anti-static precautions have been taken,
then touch a metal object that is connected to protective earth before coming near the
waste liquid container to drain static charges.
Guidelines
•Wear anti-static clothing and shoes.
•Use anti-static wrist straps to connect the human body to protective earth. For safety, the wrist strap should
be connected to the earth using an intervening resistor of about 1 M .
•Spread anti-static matting on the floor, to make the floor conductive.
Preventive Measure Four
Use tubing with an inner diameter of at least 2 mm for drain lines with high flow rates.
Guidelines
•Periodically inspect the tubing connections for leaks. Air bubbles in liquid can multiply the electrostatic charge
by a factor of 20, 30, or more.
Preventive Measure Five
If it is not possible to use a conductive waste liquid container, then follow these guidelines:
•Make sure that the end of the inflow tubing is always submerged inside of the container. Also, put metal object
that is connected to protective earth, such as a ground wire connected to the module, into the liquid.
•Use as small a container as possible to minimize damage in the event of fire.
Equipment Use and Modification
WARNING! Personal Injury Hazard. Contact the SCIEX representative if product
installation, adjustment, or relocation is required.
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Operational Precautions and Limitations

WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any purpose other than their intended
purpose can put the user at risk of harm or negatively impact system performance.
WARNING! Lifting Hazard. Make sure that at least four people or a lifting device
are available to lift the LC system. Follow established safe lifting procedures. Refer
to the Site Planning Guide for the weights of system components.
WARNING! Crushing Hazard. Wear protective footwear when moving heavy objects.
Maintenance, Inspections, and Adjustment
WARNING! Personal Injury Hazard. Contact the SCIEX representative if product
installation, adjustment, or relocation is required.
WARNING! Electrical Shock Hazard. Always turn off the power and then disconnect
the instrument prior to performing inspection and maintenance. Otherwise, fire,
electric shock, or a malfunction might occur.
To maintain the performance of the system and to obtain accurate measurement data, perform daily inspection
and periodic calibration.
•For daily maintenance and inspection, refer to Service and Maintenance.
•For planned maintenance, contact a SCIEX representative.
•For replacement parts, refer to Consumables, Options, and Spares.
•Replacement cycles described for periodic replacement parts are estimates. Replacement might be required
earlier than the described replacement cycles depending on usage environment and frequency.
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Operational Precautions and Limitations

Storage and Handling
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
The environmental requirements for the storage and transport of the ExionLCTM 100 system:
•Altitude not exceeding 1 828 m (6 000 feet) above sea level
•An ambient temperature of 4 °C to 35 °C (39.2 °F to 95 °F)
The rate of temperature change must not exceed 2 °C (3.6 °F) per hour. Ambient temperature fluctuations
exceeding these limits will result in higher signal drift and an inconsistent baseline signal from the detector.
For ambient temperatures between 4 °C and 30 °C, a non-condensing relative humidity of between 20% and
85% is required.
For ambient temperatures between 30 °C and 35 °C, a non-condensing relative humidity of between 20% and
70% is required.
Note: Do not place the system adjacent to heaters or cooling ducts, or in direct sunlight.
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Operational Precautions and Limitations

The ExionLCTM 100 system is an integrated high performance liquid chromatography system that integrates a pump
(quaternary low-pressure gradient), autosampler, and column oven and realizes a comfortable operating environment
while automating testing (validation).
This system collects the status of each unit of the pump, autosampler, and column oven for executing control. The
network function included as standard enables monitoring of the analysis status from the acquisition computer.
CAUTION: Potential System Damage. Only use the replacement parts specified in the
documentation that comes with the system. Use of any other parts might result in
instrument damage and malfunction.
Figure 2-1 ExionLCTM 100 System
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2
Overview

DescriptionItem
Controller1
Autosampler2
Pump3
Column oven4
Front Side
Figure 2-2 Front Side
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Overview