Sensa Core ST-200 aQua User manual

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OPERATOR’S MANUAL
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ST-200 aQua Operator’s Manual
1
ST-200 aQua
Na/K/iCa/Li/pH/Cl Electrolyte Analyzer
OPERATOR’S MANUAL
SENSA CORE MEDICAL INSTRUMENTATION PVT. LTD.
Plot No.3, Export Promotion Industrial Park,
Pashamylaram, Sangareddy (Dist), Hyderabad, Telangana, 502307.
www.sensacore.com, Tel: [phone].
[email protected]

ST-200 aQua Operator’s Manual
2
PREFACE
1.
Understanding the ST-200 aQua Electrolyte
Analyzer
…...
5
A.
Intended Use
…..
5
B.
Summary and Explanation
…..
5
C.
Operational Hazards and Precautions
…..
6
2.
Analyzer Installation
…..
8
A.
Unpacking
…..
8
B.
Component Installation/Replacement
…..
8
a.
Fluid control valve
…..
8
b.
Reference housing
…..
9
c.
Building the Electrodes stack
…..
10
d.
Installation of tubing
…..
11
e.
Reagent Pack Installation
…..
14
C.
Analyzer Restoration
…..
16
D.
Printer Setup
…..
17
E.
Power Up
…..
18
3.
Operating the Analyzer
…..
19
A.
Calibration
…..
19
B.
Analyze Sample
…..
21
C.
Daily Cleaner
…..
23
D.
Standby
…..
27
E.
Sub Menus
…..
28
F.
Analyze Urine
…..
32
G.
Stored Results
…..
34
H.
Reagent Pack Consumption
…..
35
I.
Quality Control
…..
36
J.
Operator Functions
…..
36
a.
Diagnostics
…..
36
b.
User menu
…..
50
c.
Setup menu
…..
59

ST-200 aQua Operator’s Manual
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d.
Parameters
…..
64
e.
Select iCa Unit
…..
65
4.
Sample Handling and Collection
…..
66
A.
Whole Blood
…..
66
B.
Serum
…..
66
C.
Plasma
…..
67
D.
Urine
…..
67
E.
Expected Values
…..
68
5.
Maintenance
…..
69
6.
Principles of Operation
…..
70
A.
ISE Theory
…..
70
7.
Specifications of Na/K/iCa/Li/pH/Cl
…..
72
8.
Troubleshooting
…..
74
Introduction
…..
74
A.
Flow path system
…..
76
B.
Flow errors
…..
77
C.
Electrode Assembly
…..
81
D.
Reagent Pack
…..
82
E.
Bubble Detector
…..
82
F.
Printer
…..
83
9.
Display Messages
…..
85
10.
Appendices
…..
90
A.
Warranty
…..
90

ST-200 aQua Operator’s Manual
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FIGURES
S/N
Figure
Figure no.
Page no.
1.
ST-200 aQua
1
7
2.
Fluid control valve
2
8
3.
Reference electrode
3
9
4.
Reference electrode removal
4
9
5.
Building the Electrode Stack
5
10
6.
Parameter combinations
6
11
7.
Pump assembly modules
7
12
8.
Pump tube installation
8
13
9.
Reagent pack installation
9
15
10.
Vent holes on reagent pack caps
10
15
11.
Printer setup
11
17
12.
Back view connection
12
18
13.
Aspirating sample
13
21
14.
Aspirating daily Cleaner
14
24
15.
Pressing Standby button
15
27
16.
Aspirating urine
16
33
17.
Correction Factor-Slope
17
57
18.
Correction Factor-Intercept
18
58
19.
Flow path system
19
76
20.
Bubble detector
20
83
21.
Flow cell cover
21
83

ST-200 aQua Operator’s Manual
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ST-200 AQUA ELECTROLYTE ANALYZER (6 Ch)
1. Understanding the ST-200 aQua Electrolyte Analyzer
A. Intended Use
The ST-200 aQua is an automated, microprocessor-controlled analyzer for
measurement of sodium, potassium, ionized calcium, Lithium, Chloride &
pH in serum, plasma, whole blood, diluted urine and cerebrospinal fluid.
To obtain accurate results, the ST-200 aQua must be operated with Our
Company’s specially packaged calibrant and bovine-based control
materials and be maintained as described in this manual.
B. Summary and Explanation
Designed for fast, efficient use, the ST-200 aQua provides clinically
accurate analysis for patient diagnosis and treatment. The analysis takes
75 seconds and requires approximately 150 micro liters of serum, plasma,
whole blood, CSF or 400 micro liters of diluted urine (65 seconds urine
analysis cycle).
You will be guided through the display menu by pressing on keypad the
“Y” or “N” button in response to questions and messages that appear on
the display. Calibration is automatic, but can be performed on demand. A
unique reagent pack contains Cal “A” & Cal “B” to calibrate the analyzer.
The ST-200 aQua Electrolyte analyzer has a external waste container for
analyzed samples and solutions, eliminating the need to handle biological
waste materials.
Many medication states the cause of temporary imbalance of the body’s
electrolyte, requires necessary treatment to the patient. Drugs for
hypertension act as diuretics, causing the body to excrete high levels of
potassium in the urine. Imbalances in the body’s potassium level affect the
neurological and muscular activity of the body. Drugs that influence
sodium concentration include all diuretics, chloropropamide, vasopressin,
antihypertensive agents and corticosteroids. Sodium imbalance is often
associated with dehydration and edema. Chloride values are seen in
metabolic acidotic states and in salt losing renal diseases.

ST-200 aQua Operator’s Manual
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C. Operational Hazards and Precautions
Read the operator’s manual before setting up or operating the ST-200
aQua electrolyte analyzer.
Observe all warnings, notes and key information in this manual.
Failure to leave the ST-200 aQua electrolyte analyzer to power with the
REAGENT PACK in place could damage the electrodes and cause blockage.
There are no operator serviceable parts inside the ST-200 aQua electrolyte
analyzer. If electromechanically problems are suspected, DO NOT attempt
to open the back cover. Contact your authorized technical service
representative.
The power cord of the ST-200 aQua electrolyte analyzer must be connected
to a matching ground outlet supplying 110 VAC, 50/60 Hz 0r 230 VAC,
50/60 Hz, as indicated on the label on the rear panel of the analyzer.
The ST-200 aQua electrolyte analyzer contains sensitive electronics and
must be properly grounded. The ST-200 aQua electrolyte analyzer should
not be plugged into a circuit protected by a GFI (Ground Fault Interrupter).
The environment should be as free as possible from dust, mechanical
vibrations, and electrical interference. Avoid proximity to brush-type
motors (certain type of centrifuges), diathermy instruments, flickering
fluorescent lights and arcing contacts of any kind. Do not install the ST-
200 aQua electrolyte analyzer near heat producing equipment or near
incandescent lighting.
BIOHAZARD
When collecting and handling biological specimens, the center for Disease
control (CDC) recommends that all samples be considered biohazards
which may be contaminated with HIV or other pathogens. Any replaceable
item which comes in contact with biological samples, including the sample
probe, solution valve, probe wipers, sample tube, sample detector,
electrodes, electrode connectors, membrane assembly, electrode housing,
pump tubing and solutions pack may contain potentially contaminated
material. Treat all components, during use and dispose, as you would use
any biohazardous material.

ST-200 aQua Operator’s Manual
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ST-200 aQua:
Figure 1

ST-200 aQua Operator’s Manual
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INSTALLATION
2. Analyzer Installation
A. Unpacking
Upon arrival, carefully remove the ST-200 aQua electrolyte analyzer and
accessories from shipping containers and place on solid work surface.
Visually inspect ST-200 aQua electrolyte analyzer for any damage
sustained during shipment. If any damage is found, please inform to your
nearest authorized dealer of ST-200 aQua electrolyte analyzer.
B. Component Installation/Replacement
To install or replace ST-200 aQua electrolyte analyzer components, follow
the procedures in this chapter. New ST-200 aQua electrolyte analyzers are
shipped with the fluid control valve and two pump motors.
a. Fluid Control Valve:
Valve is mounted in ST-200 aQua for the supply of Calibrant A, Calibrant
B solutions as per requirement. It controls the flow of Calibrant solutions
by moving the lock pin.
Valve Side View Valve Top View
Figure 2
Pinch Valve handling:
For removing or replacement of Valve should be done by using proper
star screw driver if not screw driver will damage the valve holder.
While tightening pinch valve’s screws there should not apply extra
pressure and it should not be super tight. Every time valve should be
handled gently.
Valve holder

ST-200 aQua Operator’s Manual
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b. Reference Housing:
Remove the reference electrode from its package. Wipe the outside of the
reference electrode to dry. Make sure all surfaces are clean and dry.
Reference housing consists of a KCl solution. It comes with black lock on
the seal to avoid transportation leakage of KCl Solution. Remove the black
lock part from the seal of Reference housing mandatory before installation.
Figure 3
Reference Housing: Electrode Removal
Remove the SM lock from the top of the Bubble detector and Slide the
electrode stack up at least one inch by gently pushing up on the last pin.
Figure 4
Flow Cell
Reference Seal
Internal Filling
Solution
Reference
Membrane

ST-200 aQua Operator’s Manual
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c. Building the Electrode Stack:
Assemble the electrode by following the arrow marks and bubble detector
by inserting seals in between every electrode and bubble detector. Make
sure that the arrow mark on the electrodes should indicates upper side,
each component should be clean and dry and also there should be a seal
at the bottom of electrode. Stack the electrodes as shown in fig.
Figure 5
ST-200 aQua Electrolyte analyzer comes with two blocks of 3 in 1
electrodes. The block 1 comes with two types of parameter combinations
i.e; K, Na & K, Na, iCa and the block 2 comes with 8 types of parameter
combinations as per the requirement (refer Figure 6).
Electrode housing must be in place to ensure proper operation. Upon
replacement of electrode blocks, use new electrode rubber seal. The seals
must be clean and dry.
MODEL 1 -- K/ Na
MODEL 2 -- K/ Na /Cl
MODEL 3 -- K/ Na /iCa
MODEL 4 -- K/ Na /iCa /Cl
MODEL 5 -- K/ Na /iCa /Li
MODEL 6 -- K/ Na /iCa /pH
MODEL 7 -- K/ Na /iCa /Li/pH
MODEL 8 -- K/ Na /iCa /pH/Cl
MODEL 9 -- K/ Na /iCa /Li/Cl
MODEL 10 -- K/ Na /iCa /Li /pH/Cl

ST-200 aQua Operator’s Manual
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Figure 6
Loading the Stack
Slide the electrode stack into the electrode housing. Push down firmly
until the bubble detector snaps into position.
Carefully install the bubble detector and the electrode blocks into the
electrode housing in such a manner that electrode blocks silver pins
should be inserted in female connector.
Do not force the electrode housing into place. If the electrode blocks are
properly installed, the electrode housing should slide easily into position.
d. Installation of tubing:
The ST-200 aQua Electrolyte analyzers comes with two pump motors.
The first pump motor is used for the flow of CAL-A & CAL-B solutions in to
the electrode housing in control with the valve. Its inflow is from the valve
and outflow is to the bubble detector flow cell and electrode housing.
Connect its outflow tube at the side of the flow cell pin.
The second pump motor is used for the flow of sample, QC, CAL-A & CAL-
B fluids from electrode housing to the waste bottle. Its inflow is from
electrode housing and outflow is to the waste bottle. Its pump tube has
two stoppers. One stopper (red) is connected at the base of the electrode
housing and the other stopper (blue) is connected to the waste bottle.

ST-200 aQua Operator’s Manual
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Figure 7

ST-200 aQua Operator’s Manual
13
Figure 8
Pump tube installation procedure:
1. Module base: It is fixed to the chassis through a lock at the top of
base as shown in the figure. We need to place the base bottom side
first followed by the top, which consists of lock.
2. Rollers: It consists of grove at its bottom and a knob on the front
side. Grove side should be inserted to the base knob.
3. Pump tubing: Insert the tubing as per the direction by holding the
roller firmly after applying the grease around it as shown in the
figure.
4. Front cover: It is used to lock the rollers and pump tubing as
shown in the figure. Ensure that the tag of pump tubing should be
above the front cover lock.

ST-200 aQua Operator’s Manual
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e. Reagent Pack Installation:
Reagent packs consists CAL-A and CAL-B solutions in separate containers.
Do not squeeze the reagent pack. Remove the caps and seals of the CAL-A
and CAL-B containers. Hold the reagent pack in your hand and place
reagent pack into back side slot of the analyzer; connect the CAL-A tube
cap to the CAL-A container and CAL-B tube cap to the CAL-B container
respectively as shown in below figures. Make sure that the caps should be
fixed and tighten properly.
Do not use expired reagent pack. The electrode should be exposed to
ST-200 aQua reagent all time so that it will protect electrode from getting
damaged.

ST-200 aQua Operator’s Manual
15
Figure 9
Note: Don’t place the ST-200 aQua reagent packs open to atmosphere. Because,
the solutions gets escape from the containers. Only the small vent holes on the
caps should be open for air inlet as shown below figure.
Figure 10

ST-200 aQua Operator’s Manual
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C. Analyzer Restoration:
To remove or replace any ST-200 aQua components, restore the
ST-200 aQua in following order:
Place the instrument into Maintenance mode.
MENU
1.DIAGNOSTICS
2.USER MENU
3.SETUP MENU
4.PARAMETERS
5.SELECT iCa UNIT
Press 1
↓
DIAGNOSTICS
1. DAILY CLEANER
2. MAINTENANCE
3. SENSORS
4. FLOW
5. HARDWARE
6. REAGENT PACK
Press 2
↓
ENTERING MAINTENANCE
Please wait …
Cleared liquid from the tubing
↓
To clean the flow path:
1. Enter in to maintenance mode and remove the electrode housing
by disconnecting first pump outflow tube from the bubble detector
flow cell pin and disconnect the second pump motor inflow (red)
tube from the electrode housing.
2. Remove the reagent pack CAL-A & CAL-B flow tubes from the
caps and valve.
3. Remove the first pump motor tubing from the roller. Take the DI
water in to the bold needle syringe, connect it to the tubing and
flush it with DI water and air.
To clean the electrode blocks:
If the Calibration slopes are out of range. Then, clean the electrodes
by flushing with DI water/CAL A. Do not use any plain or spring
needle to clean the electrode block.
***MAINTENANCE ***
N→ Exit

ST-200 aQua Operator’s Manual
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D. Printer Setup:
The built-in printer will provide a permanent record of calibration and
analysis results. Other data can also be requested for printing. If required,
the printer can be turned off. (To prevent damage to the printer
mechanism, the printer must be turned off if the ST-200 aQua electrolyte
analyzer runs out of printer paper.)
Refer to the following precautions:
In order to open the printer door lift the left side lock upward direction. To
install the roll of paper into the printer, cut off the beginning section of
paper that has tape or adhesives. After installing the paper, close the cover
on the top of the housing until the door gets locked.
Use of the ST-200 aQua electrolyte analyzer printer requires some
precautions:
1. Use only the printer paper provided by your ST-200 aQua
electrolyte analyzer distributor.
2. Do not permit the printer to operate without printer paper
installed.
3. In this situation go to SETUP MENU, press default setting enter
the PRINTER section and select the required option “Y” or “N” for
Printer ON /OFF. The absence of paper during printer operation
will cause premature printer failure.
4. Do not apply any lubricants, grease or other materials to the
printer assembly under any circumstances.
Failure to comply with the above requirements will void the printer
warranty.
Printer setup
Figure 11

ST-200 aQua Operator’s Manual
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E. Power Up:
Confirm that the ST-200 aQua electrolyte analyzer voltage setting on the
rear cover matches the outlet voltage. When all the components have been
assembled, plug the ST-200 aQua electrolyte analyzer into a grounded
outlet. When the ST-200 aQua electrolyte analyzer is plugged in, the power
is ON. The Na/K/iCa/Li/pH/Cl analyzer will display,
SENSACORE
ELECTROLYTE ANALYZER
Ver : XXXXXX
When the ST-200 aQua electrolyte analyzer displays SYSTEM
UNCALIBRATED PLEASE CALIBRATE, installation is complete.
After initial setup, do not unplug the ST-200 aQua electrolyte analyzer
unless it must be moved to another location.
Diagram of Back view connections:
Figure 12

ST-200 aQua Operator’s Manual
19
OPERATING THE ANALYZER
3. Operating the Analyzer
A. Calibration
The instrument has to be calibrated when it is switched ON or it
automatically calibrates on set calibrated frequency after four hours or
eight hours as per set frequency.
Following is the calibration procedure:
A. Calibration
Switch ON the instrument
↓
INITIALIZING…
↓
↓
ACQUIRING RANGES
Please Wait…
↓
SENSACORE
System Uncalibrated
Please Calibrate….
Na* K* iCa* Li* pH* Cl*
Press CALIBRATE key
↓
CALIBRATE
Y→ Continue N→ Exit
Press Y
Displays PUMP CALIBRATION STEPS
↓
↓
PUMPING AIR
↓
SELF CHECK
Searching pack