Shimadzu iMScope TRIO User manual

ZEBV-5084A
Imaging Mass Microscope
iMScope TRIO
Pre-Installation Requirements
（InstallationPreparations/Confirmation）

ZEBV-5084A
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Notice
(1) All rights are reserved, including those to reproduce this manual or parts thereof in any form without
permission in writing from Shimadzu Corporation.
Copyright 2014 Shimadzu Corporation
(2) Information in this manual is subject to change without notice and does not represent a commitment
on the part of the vendor.
(3) Any errors or omissions which may have occurred in this manual despite the utmost care taken in its
production will be corrected as soon as possible, although not necessarily immediately after detection.

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Contents
１． INTRODUCTION ···················································3
２． INSTALLATION CONDITIONS ·······························4
2.1 Carry-inroute............................................................................ 4
2.2 Example installation ................................................................ 4
2.3 Ambient requirements ............................................................. 6
2.4 Requiredpowersupply ........................................................... 6
2.5 Gas .......................................................................................... 7
2.5.1Nitrogengas....................................................................... 7
2.5.2Argongas........................................................................ 9
2.5.3General precautions........................................................ 9
2.5.4Precautionswhenusing high pressuregascylinder....10
2.6 Exhaust .................................................................................10
2.7 Other items tobe prepared.....................................................11
3．Customer pre-check list········································· 14
4．Remarks ······························································ 19
4.1Aboutmaking yoursamplesforiMScopeTRIO.........................................19

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１. Introduction
These Pre-Installation Requirements describe prior preparation required at the customer’s site in order to
facilitate installation of the iMScope TRIO. Please make sure that the content of this document is carefully
observed to ensure that the instrument function stably, providing data acquisition with a high degree of
reliability.

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2. Installation Conditions
Be sure to read the installation precautions described below to keep the instrument stable and obtain
reliable acquisition results. Also, follow the precautions given in the Safety Precautions of the instruction
manual that came with the instrument.
2.1 Carry-in route
The instrument can be moved because there are six casters on the instrument underside. Refer to the
instrument dimensions described in 2.2 Example installation so that delivery can be carried out without
problems.Also check the following points. It is recommended to have the carrier check before carrying-in.
If using an elevator, the inside dimension should be at least 2,050 mm in depth, 1,100 mm in width,
and 1,800 mm in height.Also, the frontage should be 1,100 mm in width and 1,800 mm in height.
If the instrument is carried in with a crane, prepare an approach route and the stopping place of the
crane, and the carry-in entrance to the building.
Passage and the corners in the building should have the sufficient width and height to move the
instrument.
2.2 Example installation
Front view
1710
448
397397
448
Fig.1 Example of installation layout (Front view and Top view) [mm]
Top view
1000
1980
600
(2630)
(1600)
60050
Rotary pump
Required space
RP power cable（1.5m）
230V Main power supply
cable（10m）(connect to switchboard)
Exhaust hose（5m）
(connect to duct)
Vacuum piping（1m）
Ar gas piping, N2gas piping（5m）
(connect to gas cylinder or piping
in the room)
597597
180180
Instrument
710
400 Space required for
maintenance
445
178
211
506
Portable table
(Prepared by the customer)
Control cable （0.6m）
Optical link cable（2m）
445
178 211
506 HUB
LAN cable（1m）LAN cable（1m）
*A further space of 60cm or more is needed to
the left of the iMScope to allow sufficient space
for maintenance.
*Omitted a cable of PC and a monitor and the
AC100V power supply.
PC for
analyzing PC for
acquisition
power supply.

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2250
（1380）
Left side view
Open and close view of
the front door during maintenance
Fig.2 Example of installation layout (Left side view)
Table1 Dimensions and Weight
Item
number
Item name
Dimensions
（mm）
Weight
Ｗ
Ｄ
Ｈ
（kg）
1
iMScope TRIO
1980
1000
1710
900
2
Rotary pump E2M28
597
180
276
40
3
Monitor
See the respective instruction manual.
4
PC
In the example installation given in Fig.1, a separate PC system desk has been prepared, with the
PC system placed on it.
The PC can only be placed on the left side of iMScope TRIO unit.
Install the PC within 0.6 m from the iMScope TRIO.
As the iMScope TRIO unit weights 900 kg, install on a floor that is able to stand load capacities of 900
kg in weight or more.
An additional space of 60cm or more is needed behind and to the right-hand side of the iMScope
TRIO. A further space of 60cm or more is needed to the left of the iMScope TRIO to allow sufficient
space for maintenance. Place equipment that can be readily moved, such as the PC system given in
the example, to the left of the iMScope TRIO unit, and ensure a space of 60cm or more between the
iMScope TRIO unit and walls or other immovable objects.
・Space behind iMScope TRIO unit : 60 cm or more
・Space to right of iMScope TRIO unit : 60 cm or more
・Maintenance space to left of iMScope TRIO unit : 60 cm or more
Front panel may be opened for maintenance. As referring to the figure of the example installation,
place only equipment that can be readily moved on the area where there is a possibility of
interference with the front panel.
Place rotary pump on a firm floor surface at a distance where it can be connected to the iMScope
TRIO unit by attached piping. (Extending piping to a distance greater than this will prevent
assurance of unit performance.) Ensure a distance of 60 cm or more between the back of the iMScope
TRIO and the wall, as the rotary pump will be placed on the floor behind the iMScope TRIO.
It is recommended to place the rotary pump on a stand so that oil change can be easily performed.

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2.3 Ambient requirements
This instrument is intended for precision measurement. Sufficient consideration must be given to
preparing its installation environment as a given level of performance can not be guaranteed if
installation conditions are not met.
Temperature Constant temperature of 18 to 28°C (at all times, both day and night)
Please ensure temperature does not fluctuate during the day or night. This will
ensure maintenance of unit precision. Place unit away from direct sunlight and in a
location where it will not come into direct contact with airflows from air conditioners.
Temperature stability:
+ - 1.5° C (Term - less than 1° C / 1 Hour)
+ - 0.5° C (Term - more than 1° C / 1 Hour)
+ - 1.5° C (Term –Rapid change within10minutes cycle )
(Reference) Heat discharge of this instrument: around 2,000 kcal/h
Humidity 40 to 70% (with no condensation)
Install location This instrument weighs 900 kg. Install it on a flat floor that can withstand a weight
of 900 kg or more. Ensure that there is around 2.7 m × 1.6 m of space for
installation and that the room can withstand the weight of the instrument. The
position for installation of this instrument must be at least 2.3 m high. Including
space for working, provide a W4 m D3 m H2.3 m site.
Ventilation This instrument uses flammable solvents for auto tuning, and uses large amounts of
nitrogen gas. Therefore, using the instrument in an insufficiently ventilated room
may cause oxygen deficiency. Prepare equipment that provides sufficient ventilation.
Exhaust Be sure to install duct equipment such as a draft chamber for the exhaust gas from
the rotary pump.
Other • Install in a room with minimal dust. This instrument uses a high voltage, and
dust may cause electrical discharge, damage or malfunction. Abuildup of dust in the
fan also may cause overheating.
• To maintain high performance, install in a room with minimal disturbing
elements such as vibration, electromagnetic wave noise and corrosive gases.
• Be sure to read “Safety Instructions” of the instruction manual before using this
instrument.
2.4 Required power supply
For safety reasons use a power supply equipped with a ground fault interrupter.
The iMScope TRIO unit uses AC230V, 20A, 50/60Hz (single phase) and AC100 V, 15 A, 50/60 Hz
(single phase).Avoid sharing power source with other equipment.
Acable terminal supplying AC230V to the iMScope TRIO must be connected to a circuit breaker or to
a power outlet using 3-point power plug. (Refer Fig3.)

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To avoid electric shock, ensure grounding at power supply ground terminal has a resistance of 100
ohm or less.
To assure performance, keep voltage fluctuation within ±5% and frequency stability within ±0.5 Hz.
In the country where power supply voltage is not AC100V, use a step-down transformer on the
AC100V power-supply line. Refer Fig.5 to install the step-down transformer
<Step-down transformer>
Part number
Description
Remarks
225-25315-41
STEP-DOWN TRANSFORMER 1.5KVA
The Step-down transformer and
cables are included.
Please prepare a plug.
*The number 225-25315-41 refers to the Shimadzu Corporation part number.
If power supplies of the above specifications are not available, alterations to room power supply capacity
will be necessary.
Required power supplies for each unit of the iMScope TRIO system are shown in Table 2.
Table2 required power supplies for each unit
Unit
Voltage
Current
Cable length
figure
iMScope TRIO
AC230V 50/60Hz
(single phase)
20 A
10 m
Fig.3
AC100V 50/60Hz
(single phase)
15 A
2.5 m
Fig.4
PC
See the respective instruction manual.
Monitor
Black ･･････ Connects toAC HOT
White ･･････ Connects toAC NEUTRAL
Green / Yellow ･･･ GROUND
Fig.3 Configuration ofAC230V power supply cable connection-terminal
(requires connection to circuit breaker or 3-point power plug)
Fig.4 Configuration ofAC100V power supply cable terminal
5.3mm dia.
Black
White

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OUT IN
Blue(N)
240V
230V
220V
200V
0
100V
0
a
b
P
S
Brown(L)
Yellow/Green(E)
Blue(N)
Brown(L)
Black
Earth Terminal
(Inside)
To iMScope AC100V in
To power supply
Earth Terminal
(Outside)
225-25316
STEP-DOWN TRNASFORMER 1.5K
225-25317-41
CABLE ASSY, IMSCOPE PS1
225-25319-41
CABLE ASSY, IMSCOPE PS3
225-25318-41
CABLE ASSY, IMSCOPE PS2
225-25320-41
CABLE ASSY, IMSCOPE EARTH To protective earth
Connects to either 240V, 230,V 220V, or 200V
terminal according to power supply voltage
Fig.5 How to install the step-down transformer, TBX-1.5KKV
2.5 Gas
Nitrogen gas (N2) and argon gas (Ar) are used for iMScope TRIO. Purities and pressures for each gas are
as follows:
Table3. Gas purities and pressures
Gas
Pressure supplied
to iMScope TRIO
Type
Purity
Nitrogen gas（N2）
99% or greater
(In case of nitrogen gas generator
97.0 % or greater)
690～800kPa
Argon gas（Aｒ）
99.99% or greater
200～500kPa
2.5.1Nitrogengas
The method of supplying nitrogen gas, there is a method to use liquid nitrogen, nitrogen gas cylinder,
nitrogen gas generator. This instrument does not use nitrogen gas other than stopping or adjustment.
Therefore recommend use the nitrogen gas cylinder or liquid nitrogen. The nitrogen gas generator does
not absolutely require. Please note that operating hours may be shortened depending on the flow rate of
the drying gas if using a nitrogen gas cylinder. As an approximate standard, assuming a 47L cylinder
releasing the gas at a maximum flow rate, this single cylinder would sustain the flow for approximately 5
hours. (For approximately two times of auto tuning)

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Furthermore, as the flow rate of drying gas is large, gas pressure may be smaller than expected if gas
supply pipings are long as in the case of centered gas supplies. Please take care with gas supply pipings
and pipe inner diameters as this may prevent normal control of the gas.
（a）If supplying gas generated from liquid nitrogen
Take adequate precautions with pipe resistance and other factors as sufficient secondary pressure cannot
be obtained when using nitrogen gas generated from liquid nitrogen. Prepare a PTFE pipe with an outer
diameter of 6mm and an inner diameter of 4mm or larger.
For details, refer the Pre-Installation Requirement of nitrogen generator. Various other models are
available.
・System Instruments Co., Ltd. http://www.sic-tky.com/.
・AIR-TECH Co., Ltd. http://www.airtech-corp.com/
・PeakScientific Co., Ltd. http://www.peakscientific.com/
（b）If using a nitrogen gas cylinder
Prepare the following pressure regulator and pipes given below, or equivalent products, if using a nitrogen
gas cylinder.
<Pressure regulator>
Part number
Description
Requirement
Remarks
221-35999-01*
Pressure Regulator, for N2
1 pc
Koike Sanso Kogyo Co., Ltd.
*The number 221-35999-01 refers to the Shimadzu Corporation part number.
<Piping between pressure regulator and iMScope TRIO>
The following gas supply tubes are available from Shimadzu. Please specify when ordering.
Part number
Description
Remarks
225-09933-91
Gas supply tube, external
Length : 5 m
（c）if pre-existing nitrogen gas generator is used
If the diameter of pipes leading is 1/4 inch when using an existing nitrogen generator, please use the
following adaptor.
<Union>
Part number
Description
Requirement
035-60725-07*
Half Union KQ2H07-M5
1 pc
* The number 035-60725-07 refers to Shimadzu Corporation part number.
In addition to the above part, use the following from the selection of standard accessories.
<Standard accessories>
- #62 AdaptorASSY (length 1m) (P/N: 225-15189-91*) 1pc
*The number 225-15189-91 refers to Shimadzu Corporation part number.

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2.5.2Argon gas
Use an argon gas cylinder to supply argon gas. Only a trace amount of argon gas is used (maximum
consumption 5mL/min). As an approximate standard, and assuming a 3L cylinder with a maximum flow
rate, a single cylinder will last for approximately 2 months. Gas pressure may be smaller than expected if
gas supply pipings are long. Please take care with gas supply piping length and pipe inner diameter as
this may prevent normal control of the gas.
Prepare the following pressure regulator and pipes given below, or equivalent products, if using an argon
gas cylinder.
<Pressure regulator>
Part number
Description
Requirement
Remarks
221-35999-01*
Pressure Regulator, for N2
1 pc
Koike Sanso Kogyo Co., Ltd.
*The number 221-35999-01 refers to the Shimadzu Corporation part number.
<Pipes>
The following gas supply tubes are available from Shimadzu.
Please specify when ordering.
Part number
Description
Remarks
225-09933-91
Gas supply tube, external
Length : 5 m
In addition to the above part, use the following from the selection of standard accessories.
#12 External pipe joint (P/N：225-15157-91*) 1pc
#13 Union, SS-3MO-61 (P/N：035-57501-32*) 1pc
* The numbers 225-15157-91 and 035-57501-32 refer to Shimadzu Corporation’s own
parts’ numbers.
2.5.3Generalprecautions
Take care when supplying gas by centered gas supply as other instruments being used may be
affected by the amount of gas supplied.
If using a cylinder, please use 5m or shorter pipes in general carrier gas piping. If gas supply lines are
long, the prescribed gas pressure may not be supplied to the iMScope TRIO due to pipe resistance. It
is furthermore necessary to raise the cylinder’s secondary pressure higher than the prescribed
pressure so as to ensure prescribed pressure for the iMScope TRIO.
2.5.4Precautions when usinghighpressure gascylinder
General precautions when using gas cylinders
１．Install the gas cylinder in a well-ventilated area outside the room, away from direct sunlight, and use
piping to run the gas into the room.
２．Never allow the gas cylinder to reach 40 °C or a higher temperature. Additionally, ensure that there
are no open flames within 2 m of the gas cylinder.
３．When using high pressure gas pay sufficient attention to ventilation and use soapy water or other
methods to check for leaks before starting work. When using flammable gases, in particular, do not
smoke or allow open flames within 5m of the equipment that uses the gas. Have a usable fire
extinguisher ready.
４．Secure the gas cylinder with means such as a rope so that it does not topple or fall.
５．Use oil-less pressure release valve. Do not use valves that oil has adhered to within piping that comes
into contact with gas, or in other areas.
６．Immediately shut off cylinder source valve when finished using gas.

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2.6 Exhaust
The unit emits exhaust gas from the rotary pump and vapor from mobile phase solvents.
Long-term inhalation of these is very harmful to the health.
In order to keep the site clean, it is necessary to direct these gases to a draft chamber or other such
exhaust facility. Five-meter exhaust hoses are supplied with the unit for rotary pump and drain container
discharge. Hoses from the rotary pump and the drain container must be separately connected to exhaust
ducts.Atypical draft chamber can be used.
[Example:] Draft chamber: 20m3/min.
Be sure to install the oil return kit to suppress the decrease in oil in the rotary pump and clean the
exhaust gas from a rotary pump.
< The parts which are require for the exhaust from a rotary pump>
The following options for the exhaust gas from the rotary pump are available from Shimadzu.
Please specify when ordering.
Part number
Part name
Description
225-05990-92
RP oil return kit
Kit for E2M28
018-31511
SLEEVE,PTFE 15X22 CL
Hoses for E2M28, length 1m
.
Fig6 example of exhaust piping
オイルリターン用
チューブ
オイルミストトラップ（オプション）
廃液キット
ロータリーポンプの排気
ホース（内径φ15X5）
シリコンゴムチューブ（内径φ7X5M）
（廃液キットに付属）
ダクト設備へ
廃液用容器は
お客様準備品
LCMS-IT-TOF
iMScope
Oil mist trap
Exhaust tube from Rotary pump
(ID 15*5)
Tube for oil return
Waste liquid kit
Please prepare
the container
Silicon rubber tube) inner diameter 7 ×5m
(included in Drain kit )
To duct

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2.7 Other items to be prepared
After installation, our field engineers will immediately begin to obtain adjustment acceptance data in
order to confirm normal operation of the unit.
Please prepare the following items in advance as they will be required in the operational check.
（1）Gas
Nitrogen gas N 2 Purity 99% or greater (In case of nitrogen gas generator 97.0 % or greater)
Argon gasAr Purity 99.99% or greater
（2）Standard sample for instrument adjustment
Standard sample 225-06613-08 TFANa (sodium trifluroacetate), 200mL
（3）Standard sample for Sensitivity check
Standard sample (will be informed in detail )
To perform data acquisition using a sample prepared by the customer, the following items are required in
addition to the general laboratory apparatuses. Please prepare the following items prior to lectures after
delivery of iMScope TRIO.
If the customer requested to use a sample prepared by the customer during lectures, read “4.2 About
making your samples for iMScope TRIO”before preparing. Preparing multiple glass slides with sections
on them is useful for practicing the spray coating.
（4）Environment for spray coating of the matrix
Airbrush
This is necessary for spray coating of the matrix.
Draft chamber
We recommend you use a draft chamber during the process of
spray coating of the matrix to prevent dispersion of the spray
solution of the matrix.
Dustproof / Gasproof mask
We recommend you use a Dustproof / Gasproof mask during
the process of spray coating of the matrix to prevent inhaling
the spray solution.
（5）Environment for vapor deposition-coating of the matrix(if matrix vapor deposition equipment is used)
Matrix Vapor Deposition System
This is necessary for vapor depositing of the matrix.
Recommended product : Shimadzu , iMLayer
Table for the vapor deposition
equipment
This needs to be prepared separately.
Draft chamber
If inside of the vapor deposition equipment is contaminated by
the matrix, a large amount of matrix dust may fly up when
the cover is opened. We recommend that the vapor deposition
equipment be installed on the draft chamber to prevent
dispersion of the dust.
Dustproof / Gasproof mask
If inside of the vapor deposition equipment is contaminated by
the matrix, a large amount of matrix dust may fly up when
the cover is opened. We recommend you use a Dustproof /
Gasproof mask to prevent inhaling the dust.
（6）Consumable parts
Multimeter
This is required to distinguish which is the right side of the
ITO coated glass slide. Any type of tester will acceptable as
long as it can measure the resistance value. (Required
measuring range: a few ohms to several megohms)
ITO coated glass slide
Acquisition cannot be properly performed with a normal glass

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slide. Use a glass slide with conductive surface.
Recommended product : SIGMA-ARDRICH, 578274-25P
(Pack of 25 slides)
Air duster
This is necessary for removing dust when introducing the
sample to the instrument. Use the one that does not emit any
liquid.
(Reference) The following is a list of general laboratory apparatus required after preparing a section of a
sample.
（１） Matrix spray coating
Gloves
Matrix
DHB. 9-AA, CHCA, etc
Scoopula
10mL Tube
For preparing the matrix solution.
Electric balance
For measuring the matrix.
Pipette and pipette tips
100 –1000uL
Matrix solvent
HPLC-grade methanol (This is also used for cleaning
airbrush), Milli-Q ultrapure water, TFA
Vortex mixer
For agitating the matrix solution
Ultrasonic cleaner
For agitating the matrix solution
Power extension cord
for airbrush
This is required if the airbrush power cable is too short.
Cloth
Kimwipe, etc
Black mat
This is used as a guide for measuring the coating thickness.
(not indispensable)
Aluminum foil, Tape, Scissors
This is used for masking a part of the glass slide.
（２） Vapor deposition coating of the matrix
Matrix
DHB. 9-AA, CHCA
Scoopula
Timer
In case that the vapor deposition equipment does not have a
timer function.

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Customer pre-check list
(YYYY) (MM) (DD)
The sheets on the following pages are a checklist for each of the installation conditions that have been described
in this document. Please fill out the checklist yourself and pass it on to the appropriate personnel at Shimadzu.
Customer：
Address：
TEL：
FAX：
Checked by Shimadzu
Name：

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Item
Condition
Required item
（* refers to Shimadzu
part number）
Check
1.Carry-in
route
・The instrument must be movable in
the building.
・(If using a crane) Sufficient space on
the carry-in route, the stopping
place of the crane, and the carry-in
entrance to the building is available.
□
2.Installatin
room
Temperature
18 to 28°C
*at all times, both day and night
Temperature stability :
1) + - 1.5° C (Term - less than 1° C
/ 1 Hour)
2) + - 0.5° C (Term - more than 1°
C / 1 Hour)
3) + - 1.5° C (Term –Rapid change
within10minutes cycle )
*Place unit away from direct sunlight
and in a location where it will not
come into direct contact with
airflows from air conditioners.
*(Reference) Heat discharge of this
instrument: around 2,000 kcal/h
(Depending on the situation)
・Air conditions
・Curtains at the entrance
of the installation room
・Screen to avoid direct
contact with airflows
from air conditioners
□
Humidity
40 to 70% (with no condensation)
□
Installation space
・Space for
iMScope TRIO
・Strength
・Portable table for
PC and monitor
・Stand for RP
W D H
2700mm×1600mm×2300mm
Ability to withstand weight loads
adequately. (900kg)
Refer to the example installation. The
table must be wide enough so that 2
PCs can be placed.
*Must be moveable
*The table must be place within 1m
to the left from the instrument.
Height can be raised at least 15 cm.
・Portable table for PC
and monitor（Must be
movable）
□
Miscellaneous
・Ventilation
・Interference
factors
Must be able to ventilate room well
Dust and fine particles should be minimal.
Vibration, magnetic field noise, and
other disturbances should be
minimal.
(Depending on the situation)
・Fan and/or ventilation
opening
・Curtains at the entrance
of the installation.
□
3.Power
supply
Power supply
fluctuation
(guaranteed
performance)
・Voltage
fluctuation
・Frequency fluctuation
・Within ±5% of voltage rating
・50/60Hz, ±0.5Hz
□

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Item
Condition
Required item
（* refers to Shimadzu
part number）
Check
Power capacity
・For iMScope
TRIO
・For PC, Monitor
・Ground
(ground resistor)
・AC230V 20A50/60Hz
*Avoid sharing power source with
other equipment.
AC100V 15A50/60Hz
*In the country where power supply
voltage is not AC100V, use a
step-down transformer on the
AC100V power-supply line.
・Depends on configuration
*Use a power supply equipped with a
ground fault interrupter.
・Less than 100 ohms
・Step down transformer :
STEP-DOWN
TRANSFORMER
1.5KVA
P/N 225-25315-41*
(Depending on the situation)
・Power plug for Step
Down transformer
・Extension cable for
100V line
□
Terminals
・For iMScope
TRIO
・For PC, Monitor
・AC230V：10m
AC100V：2.5m
・Depends on configuration
(Depending on the situation)
・3-point power plug for
230V
・Power strip for PC,
Monitor
□
4.Gas
N2 (nitrogen gas)
・Purity
・Supply pressure
to iMScope TRIO
・Distance from
gas supply source
・99% or greater
(In case of nitrogen gas generator
97.0 % or greater)
・690～800kPa
・Must be within 5m from the center
of instrument rear. (Use 5m pipes.)
（If using gas cylinders）
・Gas cylinder (47L
cylinder is
recommended )
・Securing means such
as a gas cylinder holder
(for two cylinder
including spare
cylinder)
・Handle for opening
and closing the gas
cylinder (if the gas
cylinder does not have
a handle)
・Pressure regulator ：
PPR-N2
P/N 221-35999-01*
・Pipes ：Gas supply
tube, external
P/N 225-09933-91*
（If supplying gas
generated from liquid
nitrogen）
・Supply through PTFE
pipe with an outer
diameter of 6mm and
an inner diameter of
4mm or larger.
□
Ar (argon gas)
・Purity
・Supply pressure
to iMScope TRIO
・99.99% or greater
・200～500kPa
（If using gas cylinders）
・Gas cylinder ( 3 - 47L
cylinder is
recommended )
□

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Item
Condition
Required item
（* refers to Shimadzu
part number）
Check
・Distance from
gas supply sour
・Must be within 5m from the center
of instrument rear. (Use 5m pipes.)
・Securing means such
as a gas cylinder holder
(for two cylinder
including spare
cylinder)
・Handle for opening
and closing the gas
cylinder (if the gas
cylinder does not have
a handle)
・Pressure regulator ：
Same as for N2
nitrogen gas
・Pipes：
Same as for N2
nitrogen gas
5.Exhaust
・Exhaust from
rotary pump
・Solvent vapor
emission from drain
・Drying gas
emissions
・Discharge evacuation gases to a
draft chamber or other such exhaust
facility. (Five-meter exhaust hoses
are supplied with the instrument.
Use of Five-meter hoses is
recommended, however, if it is too
short, prepare longer hoses. )
・Be sure to install the oil return kit to
rotary pump.
・Evacuation facilities
such as a draft chamber
・RP oil return kit
P/N 225-05990-92*
・Evacuation hose
SLEEVE,PTFE 15X22 CL
P/N 018-31511*
・Container
□
6.Miscellaneo
us
Adjustment
acceptance
・Standard samples for instrument
adjustment and sensitivity check are
required.
・CD-R (Blank) for installation data
record is required.
・TFANa 200mL
P/N 225-06613-08*
・Standard sample
(will be informed in
detail )
･CD-R (Blank)
□
Environment for
spay coating of the
matrix
・Airbrush is required.
・Also use of a draft chamber and
Dustproof / Gasproof mask are
recommended.
・Airbrush
・Draft chamber
・Dustproof / Gasproof
mask
□
Environment for
vapor
deposition-coating
of the matrix
･Table for the vapor deposition equipment
･Also use of a draft chamber and
Dustproof / Gasproof mask are
recommended.
・Matrix Vapor Deposition
System
（See the Pre-Installation of
Matrix Vapor Deposition
System）
・Draft chamber
・Dustproof / Gasproof
mask
□
Sample used for
lectures
If the customer requested to use a
sample prepared by the customer
during lectures, read “4.2 About
making your samples for iMScope
TRIO”before preparing.
・on the ITO-coated
microscope slide
(Prepared by the
customer)
□
Other required
item
・Realtime Analysis PC and Postrun
Analysis PC are sold separately.
Please prepare the items we
recommend.
・One hub and two LAN cables are
・A set of Realtime
Analysis PC
PC （1pc.）
Display（1pc.）
□

ZEBV-5084A
18
Item
Condition
Required item
（* refers to Shimadzu
part number）
Check
required to connect the above two
PCs. Please choose a length of the
LAN cable depending on placement
of the PC.
・A set of Postrun
Analysis PC
PC （1pc.）
Display（1pc.）
・Hub 1pc
・LAN cable（Strait）2pc
Others,
Consumable parts
・Multimeter is required to
distinguish which is the right side of
the ITO coated glass slide.
・ITO-coated microscope slides and air
duster are consumed regularly.
Please prepare the air duster that
does not emit any liquid.
・Multimeter (available
for measuring
resistance value)
・ITO-coated microscope
slides：（Recommended
product）
SIGMA-ARDRICH 、
578274-25P
P/N 046-25211-02*
・Air duster （The one
that does not emit any
liquid）：
□

ZEBV-5084A
19
4. Remarks
4.1 About making your samples for iMScope TRIO
(a) Back and Front of the Slide glass
The slide glass to use in the iMScope TRIO is thing called ITO glass. One side of the ITO-glass is coated
with Indium TinOxide to make it conductive. Prepare a commercial ITO coated glass slide (26 × 76 mm,
1.0-1.2 mm thick, e.g.ALDRICH #578274).
Affix the sample to the conductive side of the glass slide. It is possible to determine which surface of the
glass slide is conductive by measuring resistance with an instrument such as a tester.
(b) The Place to Mount Sections
Avoid the outermost 5 mm of either side and the outermost 1 mm (sectionB) of either end when affixing
the sample. Because of the instrument composition, we can not analyze that part.
(c) Section Thickness
To avoid the section damage and the instrument trouble, we ask you to slice the tissue within 1mm
thickness. In general, you can obtain better results with thinner sections. So, we recommend you to slice
tissues within 10µm thick.
(d) Frozen Section Attachment
When you mount frozen sections on to the ITO-glass, please make sure those sections are firmly stuck to
the glass. If a part of the section is separated from the ITO-glass, you can NOT obtain any signals from
that part. When you mount the frozen sections to the ITO-glass, please follow the method below.
Mount the frozen sections to the ITO-glass→ Touch the back of the ITO-glass with your finger→ Thaw the
section (this step makes the sections to stick firmly to the ITO-glass)→ Refreeze immediately.