Shinwa ULTRON PS-80 Series User manual

LM1099EA
ULTRON PS-80 Series
Instruction Manual
1. Introduction
Thank you for purchasing the ULTRON PS-80 series packed column for high-performance liquid
chromatography. The PS-80 series of columns are packed with a strong cation -exchange resin consisting of
sulfonated cross-linked styrene-divinylbenzene copolymer. There are 4 types of columns in the PS -80
series:
・PS-80H : designed especially for analysis of organic acids.
・PS-80N, C, P : designed especially for analysis of sugars and sugar alcohols.
ULTRON PS-80columns, which are manufactured under highly controlled conditions, must pass a series
of strict tests before being accepted for shipment. To ensure optimal performanc e and durability of the
column, please read these instructions carefully before using this column.
2. Specifications
Base material
Sulfonated styrene-divinylbenzene resin
Degree of cross-linkage
8 %
Particle size
10 m
Counter ion
PS-80H: Hydrogen (H+)
PS-80N: Sodium (Na+)
PS-80C: Calcium (Ca2+)
PS-80P: Lead (Pb2+)
column name
Dimensions
(mm)
Max. operating
pressure (MPa)
Max. operating
temperature (oC)
flow rate range
(mL/min)
PS-80H
250 x 2.0
4.0
80 (recommended 60)
0.03 ~ 0.06
PS-80H
300 x 8.0
4.0
80 (recommended 60)
0.5 ~ 1.0
PS-80N
300 x 8.0
4.0
80 (recommended 60)
0.5 ~ 1.0
PS-80N
500 x 8.0
6.0
80 (recommended 60)
0.5 ~ 1.0
PS-80C
300 x 8.0
4.0
90 (recommended 80)
0.5 ~ 1.0
PS-80P
300 x 8.0
4.0
90 (recommended 80)
0.5 ~ 1.0
3. Shipment solvent
・PS-80H ： aqueous solution of perchloric acid (pH 2.1)
・PS-80N, C, P ： pure water.
4. Mobile phase and sample
・Ensure to filter samples and mobile phases using a membrane filter with a mesh size of 0.45 µm or smaller before
using. Failure to filter mobile phases, etc. can lead to blockages of column filters and increases in analytical pressures.
・Ensure to thoroughly degas mobile phases prior to use. Insufficient degassing of mobile phases can lead to the
formation of bubbles inside analytical instruments and columns resulting in problems with analyses.
・The columns in this series are used with water or the appropriate aqueous acid mobile phases. The mobile phase may
contain up to 30% acetonitrile.
Instruction Manual

LM1099EA
5. Precautions for use
・Before installation the column, replace the liquid in the system with the mobile phase to be used.
・Tubing must have flat ends and must bottom out in the column endfitting. Tubing must be connected to the column
correctly to avoid creating a void between the column frit and tubing, which can cause a leak and result in poor
column performance.
・Install the column according to the direction of the arrow.
・Do not remove the column from LC system before the pressure drops zero.
・Flow rate direction of mobile phases is as indicated on the column label.
・Avoid using a column repeatedly near the pressure limit or making abrupt changes in pressure to prevent shortening of
the column life. The degradation of column performance is likely to occur when used at higher temperatures.
・Please flow the mobile phase at an initial flow rate 0.1 mL/min(0.01 mL/min for PS-80H 250 x 2.0) in order to avoid
the danger of sudden pressure increases.
・Metal ion and amino compounds in sample or mobile phase may degrade the column performance. Please use guard
column in order to avoid the above trouble.
・Do not subject to shock such as dropping, etc.
・Performance of the column should be carried out in accordance with the enclosed “COLUMN
PERFORMANCE REPORT”.
6. Column cleaning and regeneration
When the column performance deteriorates, try cleaning the column using regeneration solution as below at 0.5
mL/min(0.03 mL/min for PS-80H 250 x 2.0) at 50℃ for 2 hours.
(Regeneration column performance)
column name
Regeneration solution
Temperature (℃)
Procedure
PS-80H
50mM HCl
50
at 0.5 mL/min for 2 hours
PS-80N
50mM NaOH
50
at 0.5 mL/min for 2 hours
PS-80C
50mM Ca(NO3)2・4H2O
50
at 0.5 mL/min for 2 hours
PS-80P
50mM Pb(NO3)2
50
at 0.5 mL/min for 2 hours
7.Column storage
・Do not allow the column packing material to dry out.
・Close the column with end stop plugs tightly and store at cool and dark place.
・If it will not be used for a long time, flow the mobile phase at a flow rate of 0.5 mL/min (0.03 mL/min for PS-80H
250 x 2.0) for about 1 hour at least once a month.
ULTRON series packed columns are shipped under highly controlled conditions. However, if you should find any
defect, please contact your dealer or Shinwa.
Note that Shinwa does not warrant the product against column life or deterioration caused by the failure to follow the
above instructions.
50-2 Kagekatsu-cho, Fushimi-ku, Kyoto 612-8307 JAPAN
TEL [phone] URL https://shinwa-cpc.co.jp