Shoreline Biome Shoreline Breaker User manual

Shoreline Breaker™
User Manual

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Copyright
All rights reserved. No part of this book may be reproduced, stored in a
retrieval system, or transmitted, in any form or by any means (e.g.,
electronic, mechanical, photocopying, recording, or otherwise) without
the prior written permission of Shoreline Biome, LLC.
Disclaimer
Information in this document is subject to change without notice.
Shoreline Biome, LLC, makes no guarantees of any kind with regard to
this material, including but not limited to, the implied warranties or
merchantability and fitness for a particular purpose. Shoreline Biome,
LLC shall not be held liable for errors contained herein or for incidental or
consequential damage in connection with the furnishing, performance, or
use of this material.
This document and its contents are proprietary to Shoreline Biome, LLC and are intended
solely for the contractual use of its customer in connection with the use of the product(s)
described herein and for no other purpose. This document and its contents shall not be used or
distributed for any other purpose and/or otherwise communicated, disclosed, or reproduced in
any way whatsoever without the prior written consent of Shoreline Biome. Shoreline Biome
does not convey any license under its patent, trademark, copyright, or common-law rights nor
similar rights of any third parties by this document.
The instructions in this document must be strictly and explicitly followed by qualified and
properly trained personnel in order to ensure the proper and safe use of the product(s)
described herein. All of the contents of this document must be fully read and understood prior
to using such product(s).
FAILURE TO COMPLETELY READ AND EXPLICITLY FOLLOW ALL OF THE
INSTRUCTIONS CONTAINED HEREIN MAY RESULT IN DAMAGE TO THE
PRODUCT(S), INJURY TO PERSONS, INCLUDING TO USERS OR OTHERS, AND
DAMAGE TO OTHER PROPERTY.
SHORELINE BIOME DOES NOT ASSUME ANY LIABILITY ARISING OUT OF THE
IMPROPER USE OF THE PRODUCT(S) DESCRIBED HEREIN (INCLUDING PARTS
THEREOF OR SOFTWARE) OR ANY USE OF SUCH PRODUCT(S) OUTSIDE THE
SCOPE OF THE EXPRESS WRITTEN LICENSES OR PERMISSIONS GRANTED BY

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SHORELINE BIOME IN CONNECTION WITH CUSTOMER'S ACQUISITION OF SUCH
PRODUCT(S).
All names, logos, and trademarks are the property of their respective owners.
Table of Contents
Introduction 4
Intended Use 4
Safety Information 4
Installation 6
Operation 8
Maintenance 10
Troubleshooting 10
Technical Data 11
Customer Service / Technical Support 11
Warranty and Liability 12

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Introduction
Before using the Shoreline Breaker™, it is essential you read this user manual
carefully and pay particular attention to the safety information. The instructions
and safety information in this user manual must be followed to ensure safe
operation of the Shoreline Breaker™ and to maintain the Shoreline Breaker™ in
a safe condition.
The Shoreline Breaker™ has been designed to mechanically lyse biological
samples contained in bead beating tubes. Up to 48 samples in 2 ml tubes can
be simultaneously processed using Shoreline Biome’s patent-pending device
and method.
The Shoreline Breaker™ has been optimized to reduce the number of steps and
hands-on time to extract DNA from difficult-to-lyse cells, including spores, while
preserving the integrity of the genomic DNA. No programming for different
sample types or bead-beating time is required, simplifying the operation to a
single button.
Intended Use
The Shoreline Breaker™ is intended for research use-only molecular biology
applications. This product is neither intended for the diagnosis, prevention, or
treatment of a disease, nor has it been validated for such use either alone or in
combination with other products. Therefore, the performance characteristics of
the product for clinical use (i.e., diagnostic, prognostic, therapeutic or blood
banking) are unknown.
Safety Information
The Shoreline Breaker™ is designed for safe operation. However, for your
safety, the Shoreline Breaker™ should be operated as specified in this User
Manual. Failure to do so could result in injury to yourself or others, or in damage
to the instrument.
Do not submerge the device in any liquid.
Do not operate if the device is damaged in any way.
Do not attempt to modify the device in any manner. Any attempt to do so will
void the warranty.
Biological Safety
When handling biological material, use safe laboratory procedures.

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Samples may contain infectious agents. You should be aware of the health
hazard presented by such agents and should use, store, and dispose of such
samples according to the required safety regulations.
Damage to the instrument
Do not lift the instrument by holding the tube holder. It must be lifted by holding
the bottom of the unit.

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Installation
Remove all parts from the shipping container and inspect for damaged or
missing parts. If any parts are found to be damaged or missing, please contact
Shoreline Biome Technical Support.
The Shoreline Breaker™ consists of the following:
Description
Quantity
Shoreline Breaker™ base
1
Universal Power Supply
1
Plug Adapters
1 set
The power supply has been pre-assembled with the North American plug
adapter.
Identify the location where the Shoreline Breaker™ will be installed. Ensure the
surface is clear of debris, clean, and close enough to a power outlet for the cord
to reach. Avoid using extension cords or power strips.
Insert the power cable into the Shoreline Breaker™ base. (Figure 1)
Figure 1.
Place the Shoreline Breaker™ on the bench and, holding the base of the unit,
press down firmly to secure the suction cups to the bench. (Figure 2).

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Figure 2.
Do not plug the Shoreline Breaker™ in until ready to use it.
X
X

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Operation
Before using the Shoreline Breaker™, press the base of the unit to ensure that
the suction cups are fully engaged and plug the system into the wall outlet.
Prepare the samples according to the Shoreline Breaker™ Prep instructions from
the kit; see Appendix A for the list of Shoreline Breaker™ Prep kits. Ensure
each tube’s cap is fully closed before placing the tube into one of the tube holes.
Push the tube down until the lip of the tube touches the top of the Breaker™ as
in Figure 3.
Figure 3.
All tube positions mechanically lyse the sample uniformly, meaning there is no
risk of imbalance during operation.
Once all samples have been prepared and loaded into the Breaker™, push the
green button (Figure 4) to start it. The Breaker™ will rapidly agitate the tubes for
sixty (60) seconds before automatically stopping.
NOTE: The Breaker has been designed to agitate each tube for maximum lysing
efficiency. This requires a shaking motion that will result in a loud rattling
noise. This is expected as part of the standard procedure.

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Figure 4.
When finished, remove the bead lysis tubes from the Breaker™ for purification
according to the Prep Kit instructions.
“Emergency” Stop
In the event of an emergency, press the green button again to interrupt the
Shoreline Breaker™. Alternatively, the system may be unplugged to interrupt the
device.

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Maintenance
Cleaning
Unplug unit before cleaning. The housing of the unit can be cleaned with a
sponge or a damp cloth moistened with water or 70% ethanol. Be sure unit is
completely dry before plugging back into an electrical outlet.
Decontamination
If a tube breaks or spills during use, decontaminate parts that may have been
contaminated with an appropriate disinfectant. The decontamination procedure is
the sole responsibility of the user. Parts that may have been contaminated can
be cleaned with a sponge or a damp cloth moistened with 10% bleach solution.
Troubleshooting
Condition
Action
Shoreline Breaker™ does not
start
1. Check power cable at back of the
device
2. Verify power cable at wall outlet
Shoreline Breaker™ moves
during operation
1. Check that the suction cups are firmly
secured to the bench
Tubes fall out during operation
1. Confirm tubes are fully inserted into
hole, with tube lip touching the top of
the device (see Figure 4)
Figure 4
2. Check that the suction cups are firmly
secured to the bench
X

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Technical Data
Operating Conditions
Power 115 V 60 Hz / 230 V 50 Hz (autosensing)
Place of Operation For indoor use only
Storage Conditions
Temperature Store in a dry area at a temperature between
32°F and 120°F (0°C and 49°C)
Mechanical Data
Dimensions Depth: 6.2 inches (15.75 cm)
Width: 7.4 inches (18.8 cm)
Height: 5 inches (12.7 cm)
Weight 2 lb (1 kg)
Sound Level 88.2 dB
Customer Service / Technical Support
Address: Shoreline Biome, LLC
400 Farmington Avenue CB119
Farmington, CT 06032 USA
Tel: [phone]
Fax: [phone]
Email: [email protected]om
Website: www.shorelinebiome.com

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Warranty and Liability
Thank you for your purchase of a Shoreline Breaker™ instrument. Your instrument
has been carefully tested to ensure optimum operating efficiency and reproducibility
of results. Shoreline Biome warrants that all new instrumentation manufactured by
Shoreline Biome will correspond to the product specifications and be free from
defects in workmanship and materials for a period of twelve (12) months from the
original date of shipment.
Repair or replacement of defective parts will be provided to the purchaser during this
time period provided the Shoreline Breaker™ is operated under conditions of normal
and proper use, but not for damage caused by the customer. If any part or
subassembly proves to be defective, it will be repaired or replaced at Shoreline
Biome’s sole option, subsequent to inspection, provided that such defect manifested
under normal and proper use.
Limitation of warranties and remedies
THE FOREGOING WARRANTY IS SHORELINE BIOME’S SOLE AND EXCLUSIVE
WARRANTY, AND REPAIR OR REPLACEMENT OF DEFECTIVE PARTS IS THE
SOLE AND EXCLUSIVE REMEDY. THERE ARE NO OTHER WARRANTIES OR
GUARANTEES, EXPRESS OR IMPLIED. THE IMPLIED WARRANTIES OF
MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE
EXPRESSLY EXCLUDED, TO THE FULLEST EXTENT PERMITTED BY LAW.
(NOTE: SOME STATES DO NOT PERMIT DISCLAIMERS OF IMPLIED
WARRANTIES SO THIS LIMITATION MAY NOT APPLY TO YOU). WITH THE
EXCEPTION OF THE ABOVE-REFERENCED REPAIR OR REPLACEMENT
REMEDY, SHORELINE BIOME SHALL HAVE NO OBLIGATION OR LIABILITY OF
ANY NATURE WHATSOEVER WITH RESPECT TO THE SHORELINE BIOME
INSTRUMENTATION, WHETHER ARISING IN CONTRACT, TORT, STRICT
LIABILITY, OR OTHERWISE, INCLUDING BUT NOT LIMITED TO, LIABILITY FOR
INDIRECT, CONSEQUENTIAL, INCIDENTAL AND/OR SPECIAL, PUNITIVE,
MULTIPLE AND/OR EXEMPLARY DAMAGES AND/OR OTHER LOSSES
(INCLUDING LOSS OF USE, LOST REVENUES, LOST PROFITS AND DAMAGE
TO REPUTATION), EVEN IF SUCH DAMAGES WERE FORESEEN OR
FORSEEABLE, OR WERE BROUGHT TO SHORELINE BIOME’S ATTENTION. IN
NO EVENT SHALL SHORELINE BIOME’S LIABILITY TO YOU EXCEED THE
PURCHASE PRICE OF THE PRODUCT.
Liability clause
Shoreline Biome shall be released from all obligations under its warranty in the event
repairs or modifications are made by persons other than its own personnel, except in
cases where the Company has given its written consent to perform such repairs or
modifications.
All materials replaced under this warranty will be warranted only for the duration of the
original warranty period, and in no case beyond the original expiration date of original
warranty unless authorized in writing by an officer of the Company. Representations
and warranties made by any person, including representatives of Shoreline Biome,

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which are inconsistent or in conflict with the conditions in this warranty shall not be
binding upon the Company unless produced in writing and approved by an officer of
Shoreline Biome.

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Appendix A: Shoreline Breaker™ Prep Kits
The only bead-beating kits approved for use on the Shoreline Breaker™ are
listed below in Table A-1.
Prep Kit Name
Description
Catalog
Number
Shoreline Breaker™ Prep – 16
Lyse and Purify Kit, 16 Samples
BRKRPREP16
Shoreline Breaker™ Prep – 48
Lyse and Purify Kit, 48 Samples
BRKRPREP48
Table A-1. Approved Shoreline Breaker™ Prep Kits