Gilson pipetman User manual

USER’S GUIDE
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SINGLE &
MULTICHANNEL
Progress made simple!

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Contents Page
1  INTRODUCTION 3
2  PARTS CHECK LIST AND ACCESSORIES 4
3  GETTING STARTED 4
4  DESCRIPTION 5
5  PIPETTING JUST LIKEWITH PIPETMAN 6
6  PIPETTING MODES 7
7  PIPETTING SPEED CONTROL 11
8  PIPETMANTIPS 11
9  GUIDELINES FOR GOOD PIPETTING 12
10  PERSONALIZEYOUR PIPETMAN M 14
11 GOOD LABORATORY PRACTICE FEATURES 15
12 PIPETMAN M POWER MANAGEMENT 15
13  CONFIGURATION 17
14 MAINTENANCE OFYOUR PIPETMAN M 18
15 CLEANING AND DECONTAMINATION 22
16  LEAKTEST 25
17  TROUBLESHOOTING 25
18  SPECIFICATIONS 26
19 SAFETY PRECAUTIONS AND LIMITATIONS OF USE 27
20  REPLACEMENT PARTS 28
APPENDICES 29
EC DECLARATION OF CONFORMITY 33
WARRANTY 35

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1 INTRODUCTION
Congratulations on the purchase of PIPETMAN®M the natural evolu-
tion of PIPETMAN, the world standard for pipetting.
PIPETMAN M is a new motorized member of the PIPETMAN®family
from Gilson. It is a fusion of PIPETMAN’s renowned accuracy, precision
and robustness as well as user-friendly functions to simplify your
professional life.
PIPETMAN M is so easy to use that you will have the feeling that you
are using a PIPETMAN Classic:
• Push-button: set the volume by turning the button left or right and
pipette with a simple push.
• Mode-button:toaccessadditionalfeatureslikerepetitivemode,mix
function or easy readjustment.
That’s all you need to perform your pipetting tasks easily!
PIPETMAN M is nothing less than a PIPETMAN and much more:
• Easytouse: only one push-button for all pipetting functions.
• More pipette techniques for a large number of applications:
standard pipetting, repetition, mixing and reverse.
• Maximalcomfort:motorizedaspirationanddispensing,easyejection
of tips to avoid repetitive strain injuries (RSI).
• Minimaleort: PIPETMAN M is much lighter than other motorized
pipettes (RSI).
• “Nouser”eect: highreproducibility and accuracydue to motorized
pipetting.
• Morefunforbetterperformance.
PIPETMAN M is available in 10 models covering a range
from 0.5 µL to 1000 µL:
Model Reference Range Color code
P10M F81001 0.5 µL to 10 µL Red
P20M F81002 2 µL to 20 µL Pale yellow
P200M F81003 20 µL to 200 µL Yellow
P1000M F81004 100 µL to 1000 µL Blue
P8x20M F81005 1 µL to 20 µL Pale yellow
P12x20M F81006 1 µL to 20 µL Pale yellow
P8x200M F81007 20 µL to 200 µL Yellow
P12x200M F81008 20 µL to 200 µL Yellow
P8x300M F81011 10 µL to 300 µL Green
P12x300M F81012 10 µL to 300 µL Green
Push-button
Mode-button

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2PARTS CHECK LIST AND ACCESSORIES
Contents:
Just take a moment to verify that the following
items are present:
• PIPETMAN M pipette,
• PowerSupply5VwithACadapterandcable,
• 4BatteryTags,
• 4EjectorTags,
• Tipejectorextension(P10Mmodelonly),
• User’sGuide,
• QuickGuide,
• Safetybag,
• Certicateofconformity(includingbar-code
sticker),
• Lubricant(exceptforP10M,P8x20M,P12x20M,
P8x200M,P12x200M,P8x300M,P12x300M)).
3GETTING STARTED
PIPETMANMisprovidedwithaminimalbatterycharge.BeforeusingyournewPIPETMANM,westrongly
recommend you to fully charge the battery. PIPETMANMcharges80%ofitsfullbatterycapacityin
less than an hour, it takes three hours to fully charge the battery. Please refer to PIPETMANM Power
Management(Chapter12)forchargingprocedure.
Switch on your PIPETMAN M:
Pressthepush-buttontoactivatethepipette.Thestart-upscreenappears:
Thepipettewilldoaselfcalibrationtest,thermwareresetsthepiston.
During initialization, displayed on the screen, you will see the volume
range,versionofrmwareandthenumberofreadjustments.
Your PIPETMAN M is preset on PIPET mode and isnowreadytopipette
(see Chapter 5).
PIPETMANM goes into sleep mode after3 minutes of inactivity.The
display will shut o. Just press the push-button to reactivate the pipette,
your last setting and pipetting mode will appear on the screen.
Accessories:
The following items are available separately:
• POWERCARROUSEL5-positionchargingcar-
rousel for single and multichannel models
FB1001
• BatteryTags(setof4)
 F807013
• EjectorTags(setof4)
 F807014
Please contact your Gilson Distributor for
theseaccessories.
Volume range
Version of
firmware
Number of readjustments

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4DESCRIPTION
PIPETMAN M Single and Multichannel
models
Upperpart
Push-buttonandvolume adjustmentknob
Mode-button: direct access to all menu
functions
Tip ejector button
Display
Elastomer plug to cover the battery
charging port
Identity-tag clip
Identity-tag window
Connecting nut – attaches handle to
lower part
Tipejector:removabletoaccesstip-holder
 Optimizedtip-holdertoreducetiptting
and ejection forces, removable for clean-
ing and servicing
Ejector clip
Ejector support
Cover
Ejector spacer
Reset:
To reinitialize your pipette, simultaneously
press on the push- and mode- button
for at least 5 seconds
Switch O:
To switch o your PIPETMAN M, press the
push-button for at least 5 sec.
Display
The PIPETMANMdisplayisanOLEDscreen.
Usually,itshowsthecurrentmode andopera-
tionstep,pipettingvolume,batteryindicator
and piston status (aspirated and dispensed
volume) in real time.









Pipetting Mode
Indicator for aspiration
and dispense
 Batterystatus
Purge
 Volume
Warning messages appear in the
place of volume.

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5PIPETTING JUST LIKE WITH PIPETMAN
UsingPIPETMANMisaseasyasusingamechanicalPIPETMAN.YourPIPETMAN
M is set by default on “PIPETMode”, that means forward pipetting. You can
select a volume and start to pipette.
Switch on
Press push-button.
Adjust the volume setting
• HoldyourPIPETMAN M in pipette position.
• Turnthepush-buttonhalfway,thescreenwilldisplay:“clicktochangevol.”
• Nowpressthepush-button;thevolumeonthedisplaywillstartblinking,
you can now adjust the volume:
• Turn the push-button either clockwise to decrease volume or counter
clockwise to increase volume, like indicated on the button.
• Pressthepush-buttononetimewhennished.Thevolumeislocked.
Youarereadytopipette!
PIPET Mode
This is the classic pipette mode (forward pipetting), your pipette is simply
aspirating and dispensing.
Application note: You can use the PIPET Mode for all standard applications like DNA extraction, Plasmid Isolation,
Cloning, Dilution, PCR, qPCR and many others. You can easily pipette aqueous liquids like buffer, chemical solutions
(MgCl2, KCl …) and biological samples like blood, DNA and RNA.
• Fitatipsuitableforthemodelof
PIPETMAN M that you are using
(preferably use PIPETMAN TIPS for
assurance of accurate and precise
results;refertoChapter8).
• Pressthepush-buttontoaspiratetheselectedvolume.
• Todispense:
Click (press and release) the push-button.Three things happen: sample
dispense, automatic purge and piston reset to zero.
or
Keepyourthumbpressingthepush-button until theendof thedispense
cycle: The piston reset takes place after releasing the push-button again.
START button
Optional STOP
0
V
Purge
You must always fit a tip
before using any pipette!
The tip can be emptied at any time during a pipetting cycle. To do
so, turn the push button quickly: the message “click to abort” will
appearonthescreen.Clickonthepushbuttontovalidate:theliquid
will be dispensed and an automatic purge followed by piston reset
to zero will occur.

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This feature enables you to remove the tip from the liquid, without aspirating anything.
• Ejecttipbypressingtheejectionbutton.
Your PIPETMANMisreadyagain for next pipetting cycle while maintaining the volume and the last
settings!
YouhavebeenpipettinglikewithyourmechanicalPIPETMANandonlyneeded2pushesonthe
push-button!
6PIPETTING MODES
PIPETMAN M oers more pipette techniques for a large number of
applications:Youwillndallpipettingmodesinthemenu(forsystem
settingsrefertotheChapters7and13).Accessthemenubypushingthe
Mode-button. Tochooseoneofthepipettingmodes,turnthepush-
buttonandconrmyourselectionbyasimpleclick!
REPETITIVE Mode
TheREPETITIVEModeallowsyoutodistributea
samevolumerepeatedlyinpredenednumber
(N) of equal aliquots. You supply the aliquot
volume(AV)andthepipetteautomaticallycal-
culatesthenumberofaliquotspossiblefromthe
nominalvolume(NV)ofthepipetteasfollows:
N = NV/AV (e.g. 100 L x 10 for P1000M).
You also can decrease the number of repetitions (e.g. 100 L x 10-(n≥1) for P1000M).
Application note: The REPETITIVE Mode is ideal for dispensing aliquots, e.g.; dispensing a PCR master mix into PCR
tubes or 96 plates, pipetting elution buffer for DNA extraction, preparing a target for spectrometric analyses, distributing
loading buffer into samples, etc.
• Press Mode-button to access system menu, where you can choose between dierent pipette modes.
SelectREPETITIVEModebyturningthepush-button.Toconrmyourselectionclickonthepush-button.
• Turnthepush-buttonhalfway,thescreenwilldisplay:“clicktochange
vol.”
• Nowpressthepush-button;thevolumeonthedisplaywillstarttoblink,
you can now adjust the volume:
• Setthealiquotvolume:clickthepush-button,setthevolumebyturningthepush-buttonandclick
againtoconrmyoursettings.
• Setthealiquotnumber:ThemaximalnumberofaliquotshasbeencalculatedbyyourPIPETMAN
M. The number of aliquots ashes and you can decrease this number (N–(n≥1)) by turning the
push-button.Clickagaintoconrmyoursettings.
Youarereadytopipette!
Push-button
Mode-button

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1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assurance of
accurateandpreciseresults;refertochapter8).
2. Click push-button to aspirate total
volume.The volume aspirated will be
a little bit more than required (extra
volume).Theextravolumeisnecessary
to ensure equal operating conditions
for each dispensed aliquot.
3. To dispense: click the push-button.
DISCARD appears on the screen. Dis-
card the extra volume and continue
dispensing the aliquots. PIPETMAN
Mdistributes the aliquot volumeeach
time you click the push-button. The
number of aliquots left to dispense is
displayed on the screen.
A warning beep indicates the last aliquot!
4. Topurge:Pressthepushbutton,PURGE appears on the screen, click again to purge and reset the
piston. Keep your thumb pressing the push-button until the end of the purge: the piston reset
takes place after you release the push-button again. This feature enables you to remove the tip
from the liquid, without aspirating anything.
5. Eject tip by pressing the ejection button.
MIX Mode
This is the classic pipette mode (forward pipetting) followed by a mixing
phase, composed of repeatedly aspirating and dispensing, as well as an
optional forward pipetting step.
Application note:The Mode MIX can be used to prepare a PCR master mix, enzyme restriction mix, protein solution, oligo-
nucleotidedilutionandtomixsampleswithgelloadingbufferandsoon…youcanmixtwodifferentsolutionsorhomogenize
one solution. If you work with higher viscosity than water (e.g. restriction enzyme) you may change the aspiration speed
(refer to chapter 7). If you work with genomic DNA you should always pipette very carefully to avoid shearing and nicking.
• PressMode-buttontoaccessmenu,whereyoucanchoosebetweendierentpipettemodes.Select
MIXModebyturningthepush-button,toconrmyourselectionclickonthepush-button.
• Settherstvolume,namedVOLUME 1: click the push-button, set the volume by turning the push-
buttonandclicktoconrmyourselection.Thisistherstvolumeyouwillaspirateanddispense.
Set the second volume, named VOLUME2: click the push-button, you can now set the second
volumebyturningthepush-button.Clickagaintoconrmyoursettings.
You can set the VOLUME 1 or VOLUME 2= 0 µL the step will not appear. If you set the VOLUME 1 and 2 = 0 µL the
cycle will start directly with the MIX step.
• SettheMIXvolume:clickthepush-button,setthevolumebyturningthepush-button.Conrm
your settings by a simple click. It is the volume which will be aspirated and dispensed repeatedly.
“MIX” as long as you press the push-button.
You must always fit a tip
before using any pipette!
START button Optional STOP
V (+ extra vol)*
Purge
Start Purge
V (vol)
0
DISCARD ALIQUOTES PURGE
Click to Purge
Discard

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• Settheadditionalpipettingvolume,namedVOLUME 3: You can choose a volume to be aspirated
and dispensed after the mixing step, so you can continue pipetting! Click the push-button, set the
volumebyturningthepush-button.Conrmyoursettingsbyasimpleclick.
If you set the VOLUME 3 = 0 µL the cycle will stop after the MIX step.
Youarereadytomix!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN Tips for assurance of
accurateandpreciseresults;refertochapter8).
2. ToaspirateVOLUME 1 press the push-button
3. Todispense:
 Click (press and release) the push-button: Three things happen: sample dispense, automatic
purge and piston reset to zero
or
 Keep your thumb pressing the push-button until the end of the dispense cycle:The piston reset
takes place after you release the push-button again. This feature enables you to remove the tip
from the liquid, without aspirating anything.
4. Toaspirateanddispensethesecondvolume“VOLUME2”,repeatsteps2and3.
5. Tomix:Pressthepush-button;aslongasyoukeepyourthumbpressingthebutton,thepipette
continues mixing. Release the push-button to complete the current mixing cycle.
6. To purge: press push-button, PURGE appears on the screen, click again to purge and reset the
piston.
7. Topipette:IfyouhavechosenaVOLUME3>0youcannowaspirateanddispensethisvolumeby
repeatingsteps2and3.
 If you choose VOLUME1 = 0, step 2 won’t occur, if you choose VOLUME 2 = 0 µL, step 4 won’t occur, as well if you set
VOLUME 1 and 2 = 0 µL, step 1-4 will disappear. If you set VOLUME 3 = 0 µL step 7 will not occur.
You must always fit a tip
before using any pipette!
START button Optional STOP
0
V
Purge
Start Purge
Push-button pressed
V1 V2 V3

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REVERSE Mode
TheREVERSEModeislikereversemodepipettingwithamanualpipette.
During aspiration an amount of liquid is added. After delivery the excess
volume remains in the tip and is discarded.
Application note: The REVERSE Mode is ideal for pipetting viscous liquids; liquids with high vapour pressure or those
whichtend tofoam. Your PIPETMANMaspiratesa selectedvolume andan excess.Thisexcesscompensatesfortheliquid
that remains as a film inside the tip during dispensing. For example: protein extraction, cell lysis, plasmid isolation, cell
culture, buffer preparation and many others…
Electrophoresis gels loading protocol:First change the pipetting speed of your pipette (see chapter 7). Aspiration can
bedonebyusingthe standardspeed (bydefaultspeed 6).Dispensingshouldbe doneveryslowlyandcarefully, toprevent
swirling and spilling of the samples, so select the lowest speed 1. Your pipette is now ready to load your gel! Please be
aware that if you purge without pulling out the tip air bubbles could get into your gel!
• PressMode-buttontoaccesssystemmenu,whereyoucanchoosebetweendierentpipettemodes.
Select REVERSEModebyturningthepush-button,toconrmyourselectionclickthepush-button.
• Setthevolume:clickthepush-button,setthevolumebyturningthepush-buttonandclickagain
toconrmyoursettings.
Youarereadytodoreversepipetting!
1. Fit a tip suitable for the model of PIPETMAN M that you
are using (preferably use PIPETMAN TIPS for assurance
ofaccurateandpreciseresults;refertoChapter8).
2. Clickpush-buttontoaspiratetheselectedvolume.An
amount of liquid equal to the amount of purged air is
added, the volume aspirated will be a little bit more
than the set volume.
3. Clickpush-buttontodispensethevolume,theaddition-
ally amount of liquid remains in the tip.
4. Topurge:presspush-button,PURGE appears on the
screen, click again to purge and reset the piston. Keep
your thumb pressing the push-button until the end
of the purge: the piston reset takes place after you
releasethepush-buttonagain.This featureenables you
to remove the tip from the liquid, without aspirating
anything.
5. Eject tip by pressing the ejection button.
0
Purge
START button
Optional STOP Start Purge
V (+ extra vol)*
Click to Purge
Youmustalwaysfitatipbefore
using any pipette!

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7PIPETTING SPEED CONTROL
PIPETMANM is set by default on speed 6.You may need to change the speed of aspiration or dispensing
depending on your application.You can change aspiration speed and dispensing speed independently
of one other: from very slow to very fast (speed 1 – speed 6). Your speed selection is memorized for
each pipetting mode until you change it again.
• PressMode-buttontoaccesssystemmenu,whereyoucanndthedierentpipettemodes,the
speedmenuandcongurationmenu.
• SelectSPEEDbyturningthepush-button,toconrmyourselectionclickonthepush-button.
• SetASPIRATIONSPEED: aspiration speed is blinking, set the speed by turning the push-button and
clicktoconrmyourselection.
• SetDISPENSINGSPEED: dispensing speed is blinking, set the speed by turning the push-button
andclicktoconrmyourselection.
• Afterselectingthespeedyougoautomaticallybacktopipettingmode.
8PIPETMAN TIPS
PIPETMAN TIPSaremadetothehighestspecications;strictqualitycontrolismaintainedthroughout
the manufacturing process. These tips are used to calibrate PIPETMAN M, therefore for optimum per-
formance we recommend using PIPETMAN TIPS with your PIPETMAN M.
However, PIPETMAN M also oers you a high compatibility with a large
number of other tips. For more information please feel free to contact your
Gilson Distributor.
PIPETMAN TIPS have the Gilson logo engraved on their collar, ensuring that
you have a genuine Gilson product. To ensure accuracy and precision, Gil-
son’sQualityAssuranceSystemfocusesonthefollowingcriticalparameters.
• PIPETMANTIPS are made from pure polypropylene (virgin, metal-free,
toavoidthepossibilityofcontamination).Theyareavailablesterilizedandwithlters.
• SterilizedPIPETMANTIPSarecertiedfreeofdetectableRNases,DNases,DNA,RNA,andproteases.
• Optimizedshape(revisedcollarforoptimumsealing,thinwalls,andnepoint),makingthemeasier
to mount, more exible, with no vortexing, and improved precision.
• PIPETMANTIPSarefreefromevenmicroscopicdefects,especiallyattheorice.All surfaces are
smooth and hydrophobic, thereby avoiding the excessive retention of liquids that causes poor ac-
curacy and a lack of precision.
• Moldandcavityreferencesaremarkedonthecollar,ensuringthetraceability.Forqualityassurance
purposes batch numbers appear on all packages (bags and boxes).
Gilson logo ID Number

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1) FitnewPIPETMANTipforthebestresults(seeChapter18).
For single channel models, push the tip-holder into the
tipusing a slighttwistingmotion toensurea rm and
airtight seal.
For multichannel models,PIPETMANTipsarebesttted
from the patented ROCKY RACK available only in our
TIPACKs and TOWERPACKs. ROCKY RACK is the dome-
shaped part of the pack that contains the tips. ROCKY
RACKmakesiteasytosecurelytthetipstoamultichannel
pipette, ensuring an airtight seal on all channels without
the need to use undue pressure or to touch the tips.
2) Pre-rinsethetip.Pre-rinsingconsistsofaspiratingtherst
volumeofliquid and then dispensing itback intothe same
vessel (or to waste). Subsequent volumes that you pipette
willhavelevelsofaccuracyandprecisionwithinspecica-
tions. Some liquids (e.g. protein-containing solutions and
organicsolvents)canleavealmofliquidontheinsidethe
9GUIDELINES FOR GOOD PIPETTING
Aspirate and dispense
• They form an air-tight seal with the tip-holder,
preventing the leaks that cause poor accuracy and
a lack of precision.
• PIPETMANTIPS (except lter tips) may be auto-
clavedat121°Cfor20minutesat0.1MPa.
DonotautoclavePIPETMANFilterTips,they
have been sterilized!
To ensure the best performance from
your Gilson pipette, you shoud ALWAYS
use PIPETMAN Tips (in accordance with
ISO8655) system, because PIPETMAN Tips
were used to establish the specifications.
PIPETMAN Tips to use for best results
Pipette model Tips model Range Color code
P10M D10* 0.1 – 10 L Red
DL10 0.1 – 20 L
DF10 0.1 – 10 L
DFL10 0.1 – 10 L
P20M D200 2 – 200 L Pale yellow
DF30 2 – 30 L
P200M D200 2 – 200 L Yellow
DF200 2 – 200 L
P1000M D1000 100 – 1000 L Blue
D1200 100 – 1200 L
DF1000 100 – 1000 L
DF1200 100 – 1200 L
P8x20M, P12x20M DL10 0.1 – 20 L Pale yellow
DFL10 0.1 – 10 L
DF30 2 – 30 L
D200 2 – 200 L
P8x200M, P12x200M D200 2 – 200 L Yellow
D300 20 – 300 L
DF200 20 – 200 L
DF300 20 – 300 L
P8x300M, P12x300M D300 20 – 300 L Green
DF300 20 – 300 L
* A plastic adapter is required to eject D10 and DF10
tips (short tips). The adapter is supplied with P10M.
No adapter is required when using DL10 and DFL10
tips (long tips).

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- 13 -
wallofthetip;pre-rinsingthetipminimizesanyerrorsthatmayberelatedtothisphenomenon.
3) Holdthepipetteverticallyandimmersethetipintheliquid(seepage13).Pressthepush-button
toaspiratethesetvolumeofliquid.Waitafewseconds(timedependsonmodel,seepage13);
then withdraw the pipette-tip from the liquid. You may wipe any droplets away from the outside
of the tip using a medical wipe, however if you do so takecaretoavoidtouchingthetip’sorice.
4) Placetheendofthetipagainsttheinsidewalloftherecipientvessel(atanangleof10°to40°).
Pressthepush-button.Waitforatleastafewsecondsbeforereleasingthepush-buttontoexpel
anyresidualliquidfromthetip.Whileremovingthepipettedrawthetipalongtheinsidesurface
of the vessel.
5) Eject thetipbypressingrmlyonthetipejectorbutton.
General Guidelines for Good Pipetting
a.Makesurethatyoutnewtips.
b. Eachnewtipshouldbepre-rinsedwiththeliquidtobepipetted.
c.Whenaspirating,keepthetipataconstantdepthbelowthe
surface of the liquid (refer to Table).
d. Change the tip before aspirating a dierent liquid, sample, or
reagent.
e. Change the tip if a droplet remains at the end of the tip from
the previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:
 •Neverturnthepipetteupsidedown,
•Neverlaythepipetteonitssidewhenthereisliquidinthetip(s),
g.TheGilsonStandAdapter(seeChapter2)isrecommendedforusewiththeCARROUSEL and SINGLE
PipetteHoldertostoreyourPIPETMANMpipetteintheverticalposition.AlternativelythePOWER
CARROUSELcanbeusedtostoreandchargeupto5PIPETMANMpipettes.
h.Whenpipettingliquidswithtemperaturesdierenttotheambienttemperature,pre-rinsethetip
several times before use in order to reach equilibrium between the temperatures of the liquid and
the pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
Chapter 15.
k.Thepipettecanbeusedbetween+4°Cand+40°C,butthe
specicationsmayvary(seeChapter18).
l. Donotpipetteliquidshavingtemperaturesabove50°Cor
below4°C.
Immersion Depth and Wait Time
Model Immersion Depth Wait Time
(mm) (seconds)
P10M 1 1
P20M 2-3 1
P200M 2-4 1
P1000M 2-4 2-3
P8x20M, P12x20M 2-3 1
P8x200M, P12x200M 2-3 1
P8x300M, P12x300M 2-3 1
Extreme temperatures
can affect accuracy and
precision!

- 14 -
10 PERSONALIZE YOUR PIPETMAN M
Tip-ejector Extension for use with P10M
In order to eject D10 tips, tip ejector extensions are supplied with P10M
pipettes.
Thetip-ejectorextensionwhichismadeofPVDF(polyvinylideneFluo-
ride), is autoclavable.
To fit a tip-ejector extension:
• Holdthepipettewiththepush-buttonupright.
• Holdtheextensionwiththeslotupright.
• Slidetheextensionoverthetip-holder.
• Pushtheextensionrmlyontotheendofthetip-ejectoruntilitclicks
into place (see opposite).
To remove a tip-ejector extension (see opposite):
• Holdthepipetteinonehandandgriptheextensionwiththeother.
• Gentlytwisttheextension(eitherdirection)andpullitawayfromthe
pipette.
Personal Label – Name and/or Application Tag:
Your PIPETMAN M oers you two options to mark your pipette.You can
personalize your pipette with a name tag and/or an application tag:
Window-Tag:
• Remove the window by inserting a small screwdriver in the access slot.
• Position the name tag on the pipette
• Clip the window back in place.
Clip-Tag:
• Pull o the clip.
• Position the name tag into the clip
• Clamp the clip back in place.
Window-tag
Clip-tag
Short tips Long tips
To fit To remove
Slot

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- 15 -
11 GOOD LABORATORY PRACTICE FEATURES
Your PIPETMANMisfullycomplianttoISO8655standardandisCEmarked(forIVDandEMCdirectives).
PIPETMAN M includes the following GLP features.
General
• Lockablevolume.
• Usefulvolume-rangeisprintedonthepush-button.
• Volumerangeisdisplayedonthescreen.
• Serialnumberisengravedonthebody(handle)ofthepipetteandencodedinthermware.
• Barcode:ontheboxandwiththecerticate(canbetransferred).
• Personalizationbynametags(formarkingapplicationand/orusername),seeChapter10.
Cycle counters
• Fromlastvolumesetting(tocountthenumberofcyclesinthecurrent“run”).
• Frommanufactureandsincethelastservice(seeChapter14).
• Byindicators(R1,R2,R3…Rx)eachtimepipetteisreadjusted.
• Maintenanceintervalscanbespeciedbyweeksornumberofcycleselapsed.
Alarms
• Lowbatterywarning.
• Serviceisoverduewarning(seeChapter14).
• ServiceAlertcanbeset“On”or“O”.
12 PIPETMAN M POWER MANAGMENT
The PIPETMANMbatteryhasbeendesignedtoensure900pipettingcycles(750forPIPETMANMMulti
200µLand300µL)atmaximumspeed(uptonine96wellplates)betweentwochargecycles.Forser-
vice continuity when needing more capacity, your PIPETMAN M can also be used while charging with
the same performance as a fully charged pipette!
Battery Charging
PIPETMANMcharges80%ofitsfullbatterycapac-
ity in less than an hour, it takes three hours to fully
charge the battery.
Chargeyourpipettebyusingthepowersupply:
- Connect the AC adapter to a suitable AC
power supply
- Plugtheadapter into theUSB port ofthe
pipette.
- The pipette starts charging.
Stand adapter
Plug
USB port

- 16 -
Charge your pipette by using stand adapter
(seeChapter2):
- Firstplace the stand adapteron yourGilson
SINGLE pipette holder or CARROUSEL.
- Remove the plug. Now place your pipette
on the stand adapter and plug the power
transformer into the stand adapter.
- The pipette starts charging.
BatterychargingofPIPETMANM usingthe
POWERCARROUSEL:
- First install the POWER CARROUSEL as
described above.
- Remove the plug. Now place your pipette
onthechargingposition,pleaseensurethat
thepipetteisproperlyttedtothecontacts
ontthetopofthecarrousel(apropertting
will trigger a beep sound).
- The pipette starts charging.
Workingwithchargerconnectedtothepipette
PIPETMAN M can also be used for pipetting while
charging.
- Plug the power supply into your pipette.
- Press the push-button to start your pipette.
- PIPETMAN M is ready to pipette.
YoucanalsorechargePIPETMANMbyUSBwithyourcom-
puter;howeveritwilltakemuchlongerthan bypowersupply.
Low Battery Warning:
PIPETMAN M has a low battery alert. As the battery runs down, the LOW
BATTERY message appears.The warning is replaced by a blinking symbol
of an empty battery. If it is not recharged, the pipette will switch o after a
while. Settings will not be lost.
If the battery is completely discharged (= black screen, no operation) please connect the pipette to
thechargerforatleastveminutes,thena“batt.toolow”messagewillappear.Thepipettewilldisplay
the last settings used a few minutes later and will continue charging.
Changing the battery
If the following symptoms occur, please contact
your Gilson Service Center for battery change:
Never work with PIPETMAN M while
charging by computer!
PIPETMANMusesLithium-Ionbattery.
Operation on the battery is done at
user’s risk only. Opening the pipette
voids the warranty!
Use only the original power
transformer supplied by Gilson. Use
of an incompatible power supply can
damage your PIPETMAN M!

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- 17 -
 PIPETMAN M battery won’t charge or will not operate even if connected to the charger at least for
10 minutes.
 PIPETMAN M battery provides you very short cycle time or permanently needs to be used in con-
nected mode.
13 CONFIGURATION
EnterthemenuusingtheMode-button.Youwillndnotonlythepi-
pettingprograms(PIPET,REPETITIVE,MIXandREVERSE)andthespeed
controlbutalsothemenu“CONFIGURATION”,including“SERVICE”and
“ADJUSTMENT”(refertoChapter14).
ThenavigationofthemenusisalwaysdoneinthesamewayMake
yourselectionbyrotatingthepush-button,enterandconrmwith
asimpleclick!
The congurationmenu allows you to set up the following items:
1. Volumelimit: Hereyoucanxthemaximalvolumeofthepipette
(by default = nominal volume). For example you
haveaP200M pipette,butyou would liketouse itwith
your PIPETMAN DIAMOND Filter tips DF100, you can
set the maximal volume at 100 µL and you won’t risk
contamination of your pipette.
2. Beeper: You can switch the tone of your pipette on or o.
3. Contrast: You can choose between values of 1 to 5 to set up the contrast of the screen.
4. Service seeChapter14.
5. Adjustment seeChapter14.
14 MAINTENANCE OF YOUR PIPETMAN M
PIPETMANMrequiresverylittlemaintenance.However,toensurepipetteaccuracy,precisionand
robustness for timeless use please proceed periodically with a Two-minute Inspection as recom-
mended by Gilson.
Your PIPETMAN M allows you to:
• Getserviceinformation.
• Readjustthepipettetousersettings.
• Returntofactorysettings.
• Replacespareparts(fordetailedinformationseeChapters14and19).
• Preparethepipetteforcleaningorautoclavingby“disassembly”ofthepartsspecied(seeChapter15).
Push-button
Mode-button

- 18 -
Service Information
PIPETMAN M provides you with all required service information. It will help you establish an easy
diagnosis and plan any service operation with your accredited Gilson Service Center.
Youwillndinthe“service”menufollowingsubmenus:
a. Disassembly
b. Service Info
c. Service Settings
d. Pipette Info
ThenavigationofthemenusisalwaysdoneinthesamewayMakeyourselectionbyrotating
thepush-button,enterandconrmwithasimpleclick!
a.Disassembly:
The Multichannel models should not be disassembled: only the push-button and the tip-ejector can
be replaced.
OnlythelowerpartoftheSinglemodelscanbedisassembled,thepush-button,theconnectingnut
and the tip-ejector can be replaced.
This option allows you to disassemble the lower part safely (protection of the piston and the actuator).
If you select DISASSEMBLY, the following screen appears:
Now, you can remove the lower part of your pipette with complete security.
b.ServiceInfoprovidesyouwithmaintenanceinformation;youcannotmodifyanyoftheitems:
• Numberofpipettingcyclessincelastmaintenance.
• Numberofweekssincelastmaintenance.
c.ServiceSettings gives you the option to organize your maintenance, you can set following service
points:
• Numberofcyclesuntilnextmaintenance.
• Numberofweeksuntilnextservice.
• Servicedate.
• ActivateordeactivatetheServicealert.
 Changes of service settings will reset all the service info to zero.
d.PipetteInfo provides information about your pipette, you cannot modify any of the items:
• Versionofrmware.
• Serialnumber.
• Totalnumberofcycles(onecycle:upanddownofthepiston).
• Numberofreadjustmentscarriedoutbythispipette.

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- 19 -
Adjustment
Pressthemode-buttontoenterthemenu.Select“conguration”byrotatingthepush-buttonandclick
toconrmyourselection.Enterthesub-menuinthesamewayandselectthe“adjustment”menu.
The adjustment menu allows you to access the following items:
• StandardReadjustment
• ResetSettings
Standard Readjustment
Thismenuallowsyoutoadjustthepipetteusing3calibrationpoints:10%,50%and100%ofnominal
volume(inaccordingwithISO8655recommendations).
You may want to calibrate your pipette for solutions with a density, viscosity, surface tension or vapour
pressure that are dierent to that of water. To return to the factory settings, choose “ResetSettings”,
your PIPETMAN M will be reset to the three factory calibration values which are permanently stored
inthepipette’srmware.
InaccordancewithISO8655Gilsonrecommendsagravimetricprocedureforpipettecalibration.This
gravimetric method is used to establish the mean mass of a given volume of water (taking into account
evaporation losses, where necessary). After converting the mean mass to a volume (using the Z factor,
see appendix II), enter the measured volumes(s) into the pipette’s memory and the software readjusts
the pipette accordingly. This method requires the strict monitoring of environmental conditions and
the use of routinely controlled equipment that is adapted to the volume being measured.
Conversion to volume must take into account the density of the liquid as well as evaporation during
thecycletime.Foreachmeasurement,thecorrespondingvolume(Vi)canbecalculatedasfollows:
Wiistheweightasreadonthebalance
e is the mean evaporation loss during the cycle time
Z expressed in L/mg, is a conversion factor incorporating density of water buoyed in air,
at test temperature and barometric pressure (see Appendix II)
Forvolumesgreaterthan20μL,theevaporationfactorcanbedisregarded.
Formoreinformationpleasereferto“VericationProcedureforAccuracyandPrecision”whichyoucan
download from the Gilson website (www.gilson.com).
Maintenance operations
(disassembly of your PIPETMAN M to change and clean parts)
It is best to inspect your pipette regularly and to routinely
clean and change parts as required. To help you to keep
up a regular schedule and in the interests of GLP, you can
congureyourpipettetodisplayanalarmbeforeservicing
isdue(refertoServiceSettings,page18).
Vi=(Wi+e)Z
The following maintenance
operationshouldonlybedone
when the pipette is in Mode
DISASSEMBLY (see page 18)!

- 20 -
Maintenance Warning
PIPETMANMnoties you,when maintenanceis
due (service alert is set by default to“ON”).The
following message will appear:
after validation by a click, the following message
appears:
Ifyouclicktoconrmwithin10sec.thepipettewill
automatically switch to“disassembly”mode so you
can carry out your maintenance safely.
Waitlongerthan10secondsandtheoptiontohave
a reminder in one week will appear:
Clicktoconrm.Yourpipettewillbeoperationalagain.
Maintenance operation for Single channel models
 Tip-Holder and Tip-ejector
Thesepartsmustbechangedif theyaredamaged.Youmayalso
remove these parts for cleaning or decontamination purposes.
 Changing the Tip-ejector
1) Keep the tip-ejector button depressed and grip the top of
the tip-ejector with the other hand.
2) Gentlyrotatethetip-ejectorcounterclockwiseandseparate
its connector from the operating rod.
3) Pullthetip-ejectorawayfromthebodyofthepipette.
4) Cleanorautoclavethetip-ejectorandretorreplaceitby
reversing the procedure.
 Changing the Tip-holder (lower part)
After removing the tip-ejector, you may remove the lower part of the tip-holder, which is more likely
tobecomecontaminatedordamagedthantheupperpart.Removalofthelowerpartisshownbelow;
for the upper part see “Removing the Piston” (special precautions are necessary).
1) Gently rotate the lower part of the tip holder counterclockwise to unscrew it from the upper part.
2) SeparatethepartsandremovetheO-ring(see“Changing
O-ring”)
3) Cleanandifrequiredautoclavethelowerpartofthetip-
holder(autoclaving:20min.at121°Cand0.1MPa)
4) Ifrequired,lubricatethepiston(see“HowtoLubricatethe
Piston”)andtanewO-ring.
5) Screw the two parts together, making sure that the two
parts are fully tightened, by hand.
6) Retthetip-ejector.
 After autoclaving the tip-holder and tip-ejector may change color, however this has no impact on the performance.
Piston
Tip-holder
(lower)
Tip-holder
(upper)