Stalwart STM-2010MD User manual

Digital Monocular Microscope
STM-2010MD
Instruction Manual
This manual is written for digital monocular microscope STM-2010MD. To
ensure the safety, obtain optimum performance and to familiarize the use
of this microscope, it is recommended strongly that you study this manual
thoroughly before using the microscope and retain this manual in an easily
accessible place near the work desk for future reference.

1. Brief introduction
Digital monocular microscope STM-2010MD is widely used in biological, pathological,
bacteriological fields for purposes such as observation, research, academic demonstration, clinic,
medical inspection. It features more comfortable and reliable operation with modern design and
advanced technology.
2. Main Specifications
1. Mechanical tube: 160mm
2. Objectives (achromatic)：
Magnification
Numerical
Aperture
Focal Length
f (mm)
Working
distance
(mm)
4X
0.10
31.05
37.5
10X
0.25
17.13
7.316
40X
0.65
4.65
0.632
100X
1.25
2.906
0.198
Eyepiece
Objective
Stage
Coarse focusing knob
Fine focusing knob
Condenser
Main switch
Brightness adjusting knob
Digital Monocular
head
Collector
Limit bolt

3. Eyepiece
Category
Magnification
Focal Length f(mm)
Field of view
（mm）
Plan
Eyepiece
10X
24.95
Ø18
4. Total Magnification
Eyepiece
10X
10X
10X
10X
Objective
4X
10X
40X
100X
Total
40X
100X
400X
1000X
5. Objective conjugate distance:195mm
6. Stage: double layer stage 125mmX115mm
7. Coarse focusing range: 6.5mm
8.Condenser: Abbe Condenser，NA=1.2 with iris diaphragm
9.Diameter of filter：Ø28mm
10.Illuminator：LED Lamp，brightness adjustable
3. Outfit
Name
Specifications
Standard outfit
Body
Stand
Optional
Double-layer mechanical stage
Optional
Base （LED lamp）
Optional
Viewing Head
Monocular digital head
Optional
Condenser
NA1.2 Abbe Condenser
Optional
Nosepiece
Quadruple
Optional
Illuminator
LED lamp
Optional
Eyepiece
10X Plan Eyepiece
Optional
Objectives
4X
Optional
10X
Optional
40X
Optional
100X
Optional
Immersion oil
Optional
AC Adapter
Optional
User Manual
Optional
Package
Optional
4. Structure Feature
a) Eyepiece, objective and nosepiece
10X plan eyepieces combined with 4X, 10X, 40X, 100X achromatic objectives can give
the microscope a large and clear field of view. The nosepiece has a precise frame which
can locate objectives accurately and stably。 The digital monocular head can turn 360
degree freely with comforts and conveniences.
b) Curved arm and coarse and fine focusing frame
The C-type molding of the curved arm is sprightly. The coarse focusing frame adopts
dovetail guide and move smoothly with upper limit device.
c) Base
The base, with built-in illuminator, is the foundation of whole microscope which adopts
streamline circular in consonance with the curved arm.

d) Stage
Use double-layer mechanical stage, the slide is fixed with slide clip.
e) Illumination system
The illumination system consists of illuminator, iris diaphragm and condenser.
Abbe condenser can be raised and lowered by simple operation.
5. Assembly
A) Environmental Requirement：
1.Ambient temperature: 5°C to 40°C(41°F to 104°F),Maximum R.H：85%。
2. High Temperature will result in a mildewing, dew and even ruinous instrument.
3. Avoid placing the instrument in a dusty environment. When ending your microscope
operation, please cover it with the dust cover.
4. Lay the microscope in a plan and stable position, please.
B) Input voltage check:
Check the input voltage: be sure the input voltage which signed in the back of the
microscope is consistent with the power supply voltage, or it will bring a serious damage to
the instrument.
C) Eyepiece:
Insert the eyepiece into the eyepiece tube.
D) Objective:
Adjusting the coarse focus knob until the mechanical stage reaches its lower limit
position.
Screw the lowest magnification objective into the nosepiece from the left or the right
side, then revolve the nosepiece clockwise and mount other objectives in the sequence of
low to high magnification
E) Condenser:
The condenser had been installed in the microscope before leaving factory.
F) Collector:
The collector had been installed and located before leaving factory. The screw-mounted
collector can be taken down by anticlockwise turning.
H) Filter:
Mount the filter on the iris diaphragm bracket under the condenser if necessary.
6. Operation
1. Insert the eyepiece into the eyepiece tube and revolve the objectives into the nosepiece
in sequence of magnification.

2. Place the specimen in the center of the stage, keeping the cover slip upward, and then
nip it with the specimen holder.
3. Search the image by lower magnification objective, turn the longitudinal and transversal
adjustment knobs which on the mechanical ruler to move the specimen onto the center of
the field of view. Then replace with higher magnification objective, turn the fine focusing
knob to sharpen the image.
4. When using 100X oil objective, add the cedar oil between the objective and specimen.
5. You should be aware of the cleaning procedures to be used after the use of oil. Wipe oil
from the oil immersion objective, and from any other parts in need of cleaning. Clean lenses
immediately with xylene after use. Leaving oil on the lens may lead oil to seep behind and
harden on the inside of the lens.
★To obtain a bright and clear image, it is import to adjust the illumination system. The
condenser position and the iris diaphragm size should match with objectives of different
magnifications. Turn the condenser focus knob to shift the condenser. It needs to raise the
condenser when using the high magnification objective, and to decline when using the low
magnification one.
★The aperture diaphragm is used to adjust the numerical aperture instead of brightness.
Generally, setting the size of the aperture diaphragm at 70%～80% of the numerical aperture,
you can obtain a clear image with enough contrast.
★ For observing a batch of specimen of the same type, use the limit bolt to locate general
position of the stage to simplify operation. In this way, the specimen can come into focus
rapidly just by turning the coarse focusing knob.
Note: specimens of the same type means they are same in the thickness of slide, cover
slip and specimen slice.
7. Maintenance
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being
damaged.
2. All the lenses have been well checked and adjusted. It is forbidden to disassemble them by
user self.
3. The nosepiece and coarse/fine focus unit have a compact and precise frame, so please
don’t disassemble them as possibly as you can.
4. Keep the instrument clean, wipe dust regularly, apply a little non-corrosive grease in the
sliding parts and be attention to avoid contaminating the optical elements especially.
5. Place the instrument in a cool, dry position. When not using the microscope, keep it covered
with a dust helmet. Make sure the lamp socket is cool before covering the microscope.

8. Troubleshooting
Under certain conditions, performance of this unit may be adversely affected by factors
other than defects. If a problem occurs, please review the following list and take remedial
action as needed. If you cannot solve the problem after checking the entire list, please
contact your local franchiser for assistance.
1）.Optical part
Problem
Cause
Solution
The edge of the field
of view has shadow
or the brightness is
asymmetry
The nosepiece is not in the located
position（objective is not in the center
of the optical path）
Turn objective to the right
position(the optical light
center)
The image of the filament is not in the
center
Center it
The surface of the lens is moldy or has
contaminant（include condenser,
objective, eyepiece and collector）
Clean it up
Find dust and stain in
the field of view
There are stains on the lens（include
condenser, objective, eyepiece and
collector）
Clean it up
There are stains on the specimen
Clean it up
The position of the condenser is too
low
Raise it to proper
position
The image is
defocused ( low
resolution \ contrast)
There is no coverslip on the specimen
Add coverslip
The coverslip is too thick or too thin
Use the standard
coverslip ( 0.17mm )
The specimen is placed inversely
Reverse it back
There was oil on the dry
objective(easily happened in 40X
objective)
Clean it up
There are stains on the lens
（including condenser, objective,
eyepiece and collector）
Clean it up
The oil objective didn’t immerse oil
Use immerse oil
There was bleb in the oil
Eliminate the bleb
Use a unsuitable oil
Change to the specified
one
The size of the aperture diaphragm is
too large
Minify it
The size of the aperture diaphragm is
too small
Open it up
The position of the condenser is too
low
Adjust the position
One side of the
image is dark
The condenser is not in the center of
the field of view\the condenser inclines
Re-install the condenser
The nosepiece is not in the right
position
Turning it until it reach
the “clicked” position
The specimen is floating
Fix it
The image shift
during focusing
The specimen slips on the stage
Fix it
The nosepiece is not in the right
position
Turn it to the
“ clicked ”position

2）. Mechanical part
TROUBLE
CAUSE
SOLUTION
The image can focus
when using high
magnification
objective
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard
coverslip（0.17 mm）
The objective and the
specimen touch
when change
objectives from low
magnification to
higher magnification
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard
coverslip（0.17 mm）
The specimen is not
easy to move
The specimen holder is not fixed
Fix it
The eyes are too tired
The brightness is not suitable
Adjust the voltage of the
lamp
3）. Electrical Part
TROUBLE
CAUSE
SOLUTION
The lamp can’t light
when turning on the
switch
No power supply
Check the connection of
the power cord
The LED lamp is burnt out
Replace it
The lamp burns out
suddenly
The voltage is too high
Use the specified AC
adapter, if the problem is
not solved, contact with
the service department
The brightness is
not enough
Use a substandard AC adapter
Use the specified AC
adapter
The voltage is too low
Add the voltage
9．The software Scopeimage 9.0
1) Installation
1. Link the digital Monocular head with PC by the USB cable, the system will find the new
device, and then run the driver under the CD, please follow the clue to finish the installation.
Attention: please link the camera with PC before running the driver installation; and when
there comes out warning dialog, just click ‘Continue Anyway’, as the pictures show below:

2. Install the software, follow the clue to finish the installation, after the installation, there will be
a shortcut icon on the Desktop and the Start Menu.
3. Please install the video codec ‘wmv9VCMsetup.exe’, it can compress the video files when
recording, and greatly reduce the size of the video files. If you can not do record, please check
whether you have installed the video codec already.
4. Double click the shortcut icon on the desktop to preview the images, please insure the camera
has linked with the PC, and the driver has been installed rightly, or there will come out an error
hint: Cannot preview successfully. Please check your camera.
Attention:
★Please install all the three setup files in the CD, otherwise the camera may can not work
normally.
★USB2.0 is a mandatory, not optional. This camera cannot work on the computers with USB1.1
ports. In order to ensure the stability of the connection with the USB2.0 interfaces, please
insert the plug into those interfaces in the back panel the mainframe.
★ Please use the same USB2.0 port every time. Using on a different USB2.0 port requires
reinstalling or updating the driver again to make the camera work rightly. If not, it will influence the
performance of the camera, and cause the live image displays drag, for there is another system
driver, and the system set it as default driver.
★ Support operation system: Windows2000/XP/Vista 32bit, for 64bit PC processor, please installs

the 32bit operation system.
★Please unplug the USB cable from the computer immediately after using. It will shorten the
lifetime of the camera, sometimes may cause repair service if the camera is plugged in the
computer all the time and you do not shut down the computer for a long time.
2) Operation
ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It
allows you to view, capture, edit, record, zoom, measure, and process microscope images. The
main interface of the software is as follows:
Besides image windows, many other windows or user interface elements reside within the main
window of ScopeImage 9.0. These include menu, toolbars, status bar and control windows,
providing additional information about the images or control of the camera.
ScopeImage 9.0 mainly divides into two parts operation, one is for the video operation, and the
other one is for the image operation;
Video operation includes video preview, video control, color adjustment, video capture, ect.
Image operation includes image processing, image flip, image calibration and measurement, ect.
ScopeImage 9.0 is image-processing software professionally designed for digital microscope. It
allows you to view, capture, edit, record, zoom, measure, and process microscope images.
Specific features included are as follows:
1）Active image preview, freezing preview, fit to window preview, real preview, full screen
preview… various preview modes make you preview the active images with great ease.
2）Convenient color adjustment of the active image, containing brightness, contrast,
saturation, RGB value, white balance and so on, very convenient to adjust the color
during previewing.

3）Special design color database, we can associate the parameters of the color to the
database, and can store or delete the color scheme.
4）Various image measurement tools, including line, angle, rectangle and circle, can move,
delete or set the color of the objects. And you can also burn all the objects into the image
for further using.
5）Basic image operations like other similar software are included in the Image menu. You
can adjust the image at ease.
6）Image flip function, include horizontal flip and vertical flip of the active image, and rotate
90 degree, 180 degree, 270 degree or any angle degree of the captured image.
7）Image Zoom: Contain image zoom in, zoom out, 1:1.
Attention:
When launching the software, there will be several seconds displays as black preview, please
don’t worry, it is normal, because the camera needs time to do some pretreatment, to ensure the
stable working of the camera. The same reason for the recording, when you begin to record, it will
displays as black preview for several seconds, and when the images display normal, the recording
begins.
Detail information about how to operate the software, please refer to the user manual in the CD.