Stalwart STM-2093A Series User manual

STM-2093A Series
Inverted Biological Microscope
Instruction Manual
STM-2093A STM-2093AF
To ensure the safety and obtain satisfactory performance, please study this operation instruction thoroughly
before your operation.

1
ATTENTIONS
1. Please clean sample-touching part after using.
•Following the Fig.1 step to move the instrument, make sure the sample is taken away. Use
one hand holding ① position, the other hand holding ② position.
•Once the sample is damaged, must take steps to avoid pollution.
•If rising microscope’s height with other parts, must keep it in horizontal position, avoid
incline and sample slip.
Fig.1 Carrying Diagram Fig.2 Replace Bulb
a. Take Screw Off
b. Remove Lamp Cover
c. Replace Bulb
2. Before replacing the lamp, first turn off power switch at off position O, and unplug it in case of electric
shock and burn. If microscope is in use or after use, the bulb and lamp house must be cooled off
completely before proceeding, please see Fig.2, Loosening screw and replace bad bulb with required
bulb. The power supply must be cut off before bulb replacement.
3. Microscope must be placed on stable and horizontal table.
4. Only use our power line, wrong power line can’t guarantee instrument safety and performance.
5. The microscope ground terminal must be tightly connected with plug ground terminal.
6. If unexpected situation happens, please pull the plug out.
7. Disassembly only by the professionals. The microscope has been adjusted before shipping,
Unprofessional-person should not disassemble and remove any other parts.
If you have any questions, please contact with manufacturer or local distributor.
8. Wide voltage input 100～240V，47-63Hz, if not in this range, it may cause damage for equipment.
9. Don’t open microscope lower plate when in use or else exposed electrical element will lead to
electric shock. Before replacing the lamp or fuse, please turn off power switch and pull out the plug
from the socket.
10. Do not use alcohol, gasoline, paper and other combustibles near the instrument, to prevent fire! !

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MARK MEANING
Surface will be hot, don’t touch it.
Please read manual instruction carefully at first. Misuse will lead to body damage
or instrument damage.
It is near the fuse socket, means be care of electric leakage.
Main switch “ ON ”
Main switch “ OFF”
1) PREPARATION
a. Microscope is precise instrument, carefully operation, avoid collisions and shaky.
Using environment should not be in direct sunlight, high temperature or high humidity and dusty,
avoid violent shaky.
b. Tension for coarse focusing knob can adjustable.
c. Please leave enough space (10cm) for ventilation.
d. Please follow Fig.1 to carry microscope.
★Firstly, take away sample, filters and round stage parts avoid damage .
★If the microscope is leaning slightly, not in good position, rubber gasket may be fall off.
2) MAINTENANCE AND STORE
a. Please use gauze with 70% ether and 30% alcohol mixed liquor to gently wipe the lens, Alcohol
and ether are inflammable material, please take them away from fire. Be careful for turn on and
off power. Please keep indoor air ventilation.
b. Please use soft fabric to clean other parts with neutral detergent besides glass parts.
c. If not in use, please put dust cover on microscope.
d. Please collect packing material for storage and carry after unpack carton.
Safety Mark

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CONTENTS
Attentions.......................................................................................................................................................1
1. Parts Name .........................................................................................................................................................4
2. Observation Steps...............................................................................................................................................6
3. Operation............................................................................................................................................................7
3.1 Main Body.........................................................................................................................................................7
3.1.1 Turn On Light Source..................................................................................................................................7
3.1.2 Aperture Diaphragm..................................................................................................................................7
3.1.3 Usage of Filter............................................................................................................................................7
3.2 Focusing Unit ....................................................................................................................................................8
Adjustment of Coaxial Coarse Focusing Knob Tension .......................................................................................8
3.3 Observation Tube..............................................................................................................................................8
3.3.1 Adjustment Of Interpupilary Distance.......................................................................................................8
3.3.2 Adjustment Of Diopter ..............................................................................................................................8
3.3.3 Locking Eyepiece........................................................................................................................................9
3.4 Stage .................................................................................................................................................................9
3.4.1 Fixed Stage.................................................................................................................................................9
3.4.2 Mechanical Stage.......................................................................................................................................9
3.5 Condenser.......................................................................................................................................................10
3.6 Fluorescence Attachment...............................................................................................................................11
3.6.1 Mechanism of Fluorescence Production .................................................................................................11
3.6.2 Parts Name ..............................................................................................................................................13
3.6.3 Fluorescence Observation Processes.......................................................................................................14
3.6.4 Mercury Lamp Power House....................................................................................................................14
3.6.5 Replacement of Mercury Bulb.................................................................................................................15
3.6.6 Replacement of Mercury Lamp Fuse.......................................................................................................16
4. Trouble Shooting.................................................................................................................................................17
5. Installation ..........................................................................................................................................................18
5.1 Installation Diagram........................................................................................................................................18
5.2 Installation Steps.............................................................................................................................................19
5.2.1 Installation And Replacement of Bulb .......................................................................................................19
5.2.2 Replacement Of Fuse.................................................................................................................................19
5.2.3 Connecting Power Line ..............................................................................................................................19
5.2.4 Installation Of Phase Contrast Slider .........................................................................................................20
5.2.5 Installation Of Photography System...........................................................................................................20
5.2.5.1 Video System (Optional) ...................................................................................................................20
5.2.5.2 Digital Photography System(Optional) ..............................................................................................21

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Fig.3 Main Parts Name
1）Eyepiece
2）Condenser
3）Lamp Cover
4）Fixed Stage
5）Attached Mechanical Stage
6）Nosepiece
7）Main Body
1. Parts Name

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8）Seidentopf Trinocular Head
Fig.4 Control Parts
1）Eyepiece
2）Seidentopf Trinocular Head
3）Phase Contrast Slider
4）Aperture Diaphragm Lever
5）Filter Slider
6）Lamp Cover
7）Condenser
8）Round Stage
9）Fixed Stage
10）Handle
11）Objective
12）Nosepiece
13）Power Socket
14）Fuse Box
15）Power Switch
16）Surplus Water Plate
17）Attachable Mechanical
Stage Knob
18）Potentiometer Knob
19）Coarse & Fine Focusing Knobs
20）Attachable Mechanical Stage
21）Switch Knob

Please read chapter 6 about assembly steps.
2. Observation Steps
Putting objective in optical path and focusing.
Turn filter into
optical path.
Adjusting aperture diaphragm
Adjusting brightness
Observation
Turn on main power source
Place sample
Put 10X objective in optical path
Focusing sample
Adjusting brightness
Adjusting interpupilary
distance and diopter
6

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3.1.1 Turn On Light Source (Fig. 5)
a. Turn potentiometer knob ① to the minimum, turn on power switch to ② position I(ON).
b. Rotating potentiometer knob ① to increase and decrease brightness for good illumination.
Fig. 5 Power Switch Fig.6 Adjustment of Aperture Diaphragm
3.1.2 Aperture Diaphragm (Fig.6)
Gently moving diaphragm lever ① to adjust aperture diaphragm for best image contrast.
3.1.3 Usage of Filter
a. Take out the filter holder ②.
b. Insert filter① into filter holder ②and pull filter holder into the socket.
Fig.7 Filter Installation
3. Operation
3.1 Main Body

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Adjustment of Coaxial Coarse Focusing Knob Tension (Fig.8)
a. Coarse focusing knob tension can be adjustable, hold adjustable ring ① and rotate it. Anti-clockwise
direction is decrease, clockwise direction is increase.
b. If stage is decreasing automatically, the sample is deviating from the focusing point, means tension
too low, clockwise rotating adjustable ring ① to increase tension.
Fig.8 Tension Adjustment For Coarse Focusing Knob
Don’t rotate coarse and fine focusing knobs with reverse direction at the same time.
3.3.1 Adjustment of Interpupillary Distance
Please adjusting binocular tube to make left and right field coincide completely. Indication point ●is
interpupillary distance.
Fig.9 Adjustment of Interpupillary Distance Fig.10 Adjustment of Diopter
3.3.2 Adjustment of Diopter (Fig.10)
Please turn adjusting ring scale 0to scale line at the first use.
3.2 Focusing Unit
3.3 Observation Tube

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a．With right eye to observation by right eyepiece, focusing the sample by coarse and fine focusing
knob.
b．With left eye to observation by left eyepiece, focusing the sample by coarse and fine focusing knob.
3.3.3 Locking Eyepiece
Eyepiece can be tightened by using thumb screw ①(Fig.11). If want to change eyepiece, must unscrew it
at first.
Fig.11 Locking Eyepiece
3.4.1 Fixed Stage
a. Insert standard round plate○
1in stage.
b. Install the clamps 2 on the stage by screw joint.
Fig.12 Fixed Stage
3.4.2 Mechanical Stage
a. Mechanical stage can be moved freely at X and Y direction.
b. Insert standard stage round plate 1 in stage. (also don’t insert round plate as needed).
c. Install attached mechanical stage 2 in stage with knurled screw 3 or hexagon socket cap screws.
d. Put stage plate 4 in stage as needed and fixed with clamps.
3.4 Stage

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Fig.13 Mechanical Stage
◎According to culture dish size to decide if remove condenser.
a. Use hexagon socket screwdriver to loosen thumb screw ① can remove condenser②.
b. When removing condenser, please hold condenser② with hand in case of broken.
Fig.14 Condenser Installation
3.5 Condenser

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◎STM-2093 inverted biological microscope can configured with fluorescence attachment
(lamp
house, power supply etc.)
3.6.1 Mechanism of Fluorescence Production
Ultraviolet(U), Violet(V), Blue(B), Green(G) transmittance characteristic curve.
UV ( Ultraviolet )Excitation V (Violet) Excitation
B( Blue Light )Excitation G (Green Light )Excitation
Fluorescence is a kind of photoluminescence in natural world. This kind of material will emit more longer
wavelength light than irradiation light wavelength, if stop light, emitted light will be disappeared. The longer
wavelength is fluorescent light. The substance which can launch the fluorescent is called fluorescence material.
Stimulate the principle: Now use blue-ray as example (B light, the wavelength of 490nm), when the light is
emitted from the DC mercury lamp, after condenser and stimulated color filter, get the exciting light wavelength
of 490 nm or so, then through color spectroscope reflection into the objective, color spectroscope has the nature
of selective reflection in a certain spectrum area. It will completely reflect all light which is shorter than a certain
wavelength and all light which is longer than a certain wavelength of light will totally through it. According to this
feature, color spectroscope can be designed corresponds to the excitation light beam splitter, it reflects the light
of wavelength under 490 nm, and the wavelength is more than 490 nm light can through. So such a wavelength
of 490 nm or so exciting light after color spectroscope, greater than 490 nm light through color spectroscope to
be removed, and the excitation under 490 nm light irradiation by fluorescence objective can converge on the
fluorescent specimens, specimen inspire greater than 490 nm wavelength of fluorescence(effective fluorescence
wavelength of 525nm) and fluorescence imaging by objective gathering, and through the color spectroscope
3.6 Fluorescence Attachment

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(with a small amount of under 490 nm exciting light is reflected back to the light source and not through) and
cut-off filter (cutoff wavelength less than 525 nm light), pure fluorescent light beam for observation and
photography.
Fluorescent Attachment Diagram
Fig.15 Fluorescent Attachment Diagram
1) Mercury Lamp
2) Condenser
3) Excitation Filter
4) Specimen
5) Objective
6) Color Spectroscope
7) Filter for Resistance
8) Prisms Group For Inverting Light Path Direction
9) Eyepiece

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3.6.2 Parts Name
Fig.16 Parts Name
1) Eyepiece
2) C-mount
3) Digital Camera
4) Filter
5) Lamp Cover
6) Phase Contrast Slider
7) Condenser
8) UV Protecting Plate
9) Stage Plate
10) Mercury Lamp
11) Attached Mechanical Stage For 96-hole Plate
12) Nosepiece
13) Power Switch
14) Mechanical stage hand knob
15) Diaphragm Slider
16) Fluorescent Transforming Handle
17) Potentiometer Knob
18) Coarse & Fine Focusing Knobs
19) Tension Adjusting Knob

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20) Main Body
21) Trinocular Lever
22) Seidentopf Trinocular Head
3.6.3 Fluorescence Observation Processes
According to bright field observation methods to adjust microscope, take following steps to make
observation:
●Put mercury lamp power supply power plug into the power socket, please firstly check the power
supply voltage whether conform to the requirements of the instrument and supply voltage.
●Open the mercury lamp power switch, mercury lamp power voltage fluctuation may not be greater
than 220V ±20V ,otherwise affect the start of mercury lamp. It takes about 10 minutes to reach stable
state, the maximum luminous efficiency.
●Put 10X objective in optical path. Put fluorescent specimen on the slide, with fixed clamp, regulating
stage vertical moving handle to make the objective in the optical path.
●Through push and pull the fluorescent transform handle, fluorescence excitation cube will be moved
into light path .
●Adjusting coarse and fine focusing knob to get clear image.
●Adjusting the center of the low and high pressure mercury lamp.
After getting clear image by focusing , using condenser axial adjustment knob, mercury lamp arc
light can be seen in the eyepiece . If arc light regiment not in the center of the field, by adjusting the
vertical and horizontal adjustment knob of mercury lamp light box to make the center of the arc light
group in the field of view, adjusting the mirror, make the brightness to the brightest. When arc light
group in the center, adjusting condenser axial adjustment knob to make arc light group into the whole
field, fluorescence observation can be made.
Try to avoid frequent open mercury lamp power supply, it will reduce mercury lamp working life.
Once closed, mercury should be restart after five minutes later.
3.6.4 Mercury Lamp Power House
a) Main Parameter
Input Voltage: AC 100 ~240 V 47~63 Hz
Output Voltage: DC18V~ 40V Maximum
Stable Output Current: 3.6A ～ 4.9A
Start Stable time: 2 minutes
Fig.17 Adjustment for Mercury Lamp House
1) Condenser Adjusting Knob
2) Vertical Adjusting Knob for Mercury lamp
3) Horizontal Adjusting Knob for Mercury lamp
b) Use of Power Supply

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● Determine the input voltage is conform to the requirements of the instrument, can open the
main power switch, open the indicator lights on the rear panel is light, indicating the power switch
on.
●Gently press the trigger switch, power box above positive press switch in place can let go, don't
have to hold for a long time. If you don't can trigger mercury lamp, please check the mercury lamp
light boxes closed is complete.
● Mercury lamp light will be stable after 2 minutes commonly, lamp power consumption between
90 W and 135 W.
● Mercury lamp service life is 100 hours or so, so use for a period of time after the brightness will
decrease.
● After replacing mercury lamp, power box timers need to reset, pressing the reset which on the
front side of power box.
● Don't often press the power box timer reset button, also can't often time, according to otherwise
it will affect the service life.
● Don't no-load test output voltage, also don't open source box to adjust or modified, in order to
avoid damage to mercury lamp or power box.
Fig.18 Front Side Fig.19 Back Side
1) Timer 2) Reset 3) Trigger Switch 4) Power Switch 5) Output Socket 6) Cooling Fan
7) Voltage Transfer Switch 8) Input Power Plug 9) Fuse Holder

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3.6.5 Replacement of Mercury Bulb
Cut off the power supply, with inner
hexagon screwdriver to adjust the socket head
screw which in the rear of the mercury lamp light
box, then slowly remove the mercury lamp light
box upper portion, upside down on top of the
desktop. Unscrew two mercury lamp set screw,
remove the old mercury lamp, change new
mercury lamp, then tighten the two fixed screw
will recover lamp holder, open screw tightening
the mercury lamp holder. Then according to the
mentioned methods for mercury lamp in front of
the center.
Fig.20 Replacement of Mercury Lamp
●Don’t remove mercury lamp under the condition of electricity from inside the box! Danger!
●Mercury lamp must be replaced after cooling.
●Note mercury lamp installation in negative direction, and the lamp glass shell on uneven parts from
the direction of the condenser.
●With gauze dipped in a little alcohol ether 4:6 mixture to wipe the lamp glass shell surface, there is no
dust, fingerprints, etc contamination tubes.
3.6.6 Replacement of Mercury Lamp Fuse
Turn off the power, pull out the plug and open fuse box .Mercury power supply fuse:
Φ5×20mm, 5.00A, 250V (The detail steps are as follows).
1.
2.
3.
Fig.21 Replacement Of Power Box Fuse Tube

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Trouble Causation Remedy
1. Optical System
a. Field incomplete or
illumination irregular.
Nosepiece not in right location. Readjust nosepiece in right position.
Filter slider not in right place. Readjust filter slider
Phase Contrast Slider aren’t moved out of optical
path. Moving out the slider.
b. Dirt or dust in objective field.
Lens of collector is dirty.
Thoroughly clean.Sample is dirty.
Eyepiece is dirty.
c. Dazzling image Aperture diaphragm isn’t wide enough. Readjust it.
d. Bad Image quality, for
example, not sharp, low
picture contrast, image
detail not clear.
Objective isn’t in right position. Rotate nosepiece to locating position.
Objective front lens is dirty. Thoroughly clean.
Culture dish bottom is thick. Use normal size culture dish.
Culture dish optical performance isn’t good. Use standard culture dish.
Sample is dirty. Thoroughly clean
e. Partial image isn’t clear or
unsteady.
Objective isn’t in right position. Rotate nosepiece to locating position.
Sample isn’t placed at right stage place. Correctly placing sample and fixer it
with holder.
f. The effect of phase contrast
observation image is poor.
Annular spot don’t focus with dish holder. Readjust it.
Culture dish bottom isn’t flat. Use standard culture dish.
2. Mechanical Focusing Unit
a) Coarse focusing knob is too
tight.
Tension too big. Loosing tension adjusting ring ，
reset again.
b) Nosepiece glide down
automatically.
Tension too small. Tightening tension adjusting ring,
reset again.
3. Binocular Observation Tube
Field of Binocular observation
tube isn’t inconsistent.
Wrong interpupillary distance. Correctly set again.
Binocular diopter is incorrect.
Left eye and right eye with different eyepiece. Change one eyepiece, to make it
same as another eyepiece.
4. Nosepiece
When using high power objective,
it will touch the sample. Improper sample. Use right sample.
Culture dish bottom is too thick. Use standard dish.
5. Power System
a) Bulb don’t work. No bulb. Install bulb.
Bulb or fuse is broken. Change new bulb or fuse.
Don’t plug in power. Plug in power safely.
b) Bulb is easy broken. Don’t use specified specification bulb. Use specified specification bulb.
No bulb. Install bulb.
加装机械载物台
4. Trouble Shooting

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Fig.22
5. Installation
5.1 Installation Diagram

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5.2.1 Installation and Replacement of Bulb (Fig.2)
WARNING: Cut off the power line plug before replace bulb.
a. Completely loosening screw on the top of the lamp house, pulling out lamp shade.
b. Loosening bulb pins locking screw, using glove or gauze to hold the bulb and insert fully
into the socket.
c. Install the lamp again.
◎Bulb: 6V30W Halogen
5.2.2 Replacement of Fuse
WARNING：Turn off the power plug before replacement.
Fuse box is installed below the power socket, fuse rating: Φ5X20mm, 5A/250V.
Fig.23 Replacement Of Fuse
5.2.3 Connecting Power Line
a. Don’t curve or twine power line.
b. Make sure the main switch at off mark O when
connecting power lines.
c. Must use three-phase plug which can connect ground
electrode, Otherwise can’t use the microscope.
If power line is close to lamp house or relevant device, it
will melt and cause electric leakage. So don’t close to
lamp house.
Fig.24 Power cord Connection
5.2 Installation Steps