Stalwart STM-2092 User manual

Inverted Biological
Microscope
Model Number
STM-2092
Instruction Manual
To ensure correct operation, please read this manual thoroughly before using the
microscope and keep it near the product for easy reference.

1
CONTENTS
User notice ......................................................................................................................................3
1. Application............................................................................................................................3
2. Safety Precaution ..................................................................................................................3
3. Maintenance and Storage......................................................................................................4
4. Safety Symbols .....................................................................................................................5
I. Nomenclature ..............................................................................................................................6 
2. Installation...................................................................................................................................6
2-1 Installation Diagram ...........................................................................................................6
2-2 Installing steps....................................................................................................................7
2-2-1 Installing and replacing the lamp（Fig.4）...................................................7
2-2-2 Installing the lamp house（Fig.5）...................................................................8
2-2-3 Mounting the objectives（Fig.6-7）..................................................................8
2-2-4 Installing the stage lengthen splint and the mechanical ruler（Fig. 8）....10
2-2-5 Installing the stage inserted plate（Fig.9）...................................................10
2-2-6 Installing the eyepiece.......................................................................................10
2-2-7 Installing the color filters ...................................................................................11
2-2-8Connecting the power cord .........................................................................12
3. Controls .....................................................................................................................................13
4. Using the controls ....................................................................................................................14
4-1 Microscope Frame............................................................................................................14
4-1-1 Turning on the lamp...........................................................................................14
4-1-2 Adjusting the brightness( Fig.16）...................................................................14
4-1-3 Adjusting the tension of the coarse .................................................................14
adjustment knob（Fig.17）........................................................................................14
4-2 stage..................................................................................................................................15
4-2-1 Placing the specimen ........................................................................................15
4-2-2 Moving the specimen ....................................................................................................16
4-3 The viewing tube ..............................................................................................................16
4-3-1Adjusting the diopter ...........................................................................................16
4-3-2 Adjusting the interpupillary distance................................................................17
4-3-3 Selecting the light path......................................................................................17
4-4 Illumination.......................................................................................................................18
4-4-1 Using color filters................................................................................................18
4-4-2 Using the aperture diaphragm .........................................................................19
4-4-3 Removing the condenser lens..........................................................................20
5. Phase Contrast Observation ..................................................................................................20
5.1name of components ..........................................................................................................20
5-1-1 Phase contrast objective...................................................................................20
5-1-2 Phase contrast slider .........................................................................................21
5-2 Installation and usage .......................................................................................................21
5-2-1 Installing the phase contrast slider ..................................................................21

2
5-2-2 Centering the light annulus...............................................................................22
6. Microscope photography and video ......................................................................................23
6-1 Microscope video..............................................................................................................23
6-1-1 Selecting the light path（Fig.31）....................................................................23
6-1-2 Installing the video set.......................................................................................24
6-2 Microscope photography ..................................................................................................24
6-2-1 selecting the light path.......................................................................................24
6-2-2 Installing the photography set (Fig.33) ...........................................................24
6-1-3 Focus ...................................................................................................................24
6-2-3 Focus ...................................................................................................................25
6-2-4 Adjusting the color temperature............................................................................25
6-3 Digital photography..........................................................................................................26
6-3-1 Selecting the light path......................................................................................26
6-3-2 installing the photography set ..........................................................................26
7. Specifications ...........................................................................................................................27
7-1 Main specifications...........................................................................................................27
7-2 Objective Specifications...................................................................................................28
8. Configuration table for inversed microscope STM-2092....................................................28
9. Troubleshooting .......................................................................................................................
29


3
User notice
1. Application
Inversed Biological microscope is the dedicated microscope for biological and
medical areas, applied in the field of microscopic measurement, health agencies,
laboratories, research institutes and universities and other units for biology, genetics,
immunology, chemistry, environment protection, oceanography, pharmacology, bacterial
observation, education and professional studies.
2. Safety Precaution
1. Do not keep the instrument in a direct sunlight, high temperature or humidity,
dusty and easy shaking environment. Make sure the stage is plane, horizontal
and stable enough.( Weight: about 9.5 kg)
2. When moving the microscope, please hold the instrument by the lower side of the
observation tube④and the illumination column②.（Fig.1）
3. If the bacterium solution or the water splash to the stage, objective or viewing
tube, set the main switch to off state and unplug the power cord. Then wipe away
Fig.1

4
any liquid. Otherwise, the instrument will be damaged.
4. When working, the lamp house on the top of the arm③(Fig.1) will become very
hot, be sure there is enough room around the lamp house (especially the top side
for cooling).
5. Before replacing the lamp bulb or fuse, turn the main switch①to the “O”(off)
position, then cut off the power. If the lamp is on, or soon after it has been turned
off, it is hot and will cause serious burns, please do the replacement after it cool
down completely.
★ Specified lamp：the halogen lamp 6V30W（PHILIPS5761）
6. Earth this instrument to prevent the lightning strike.
7. Use the specified power cord, please.
8. The product should stored in a shady location and no acidic gases, alkalis, organic
solvents and other hazardous materials surrounding; the storage period is usually not
more than 6 months.
★always ensure that the grounding terminal of the microscope and that of the wall
outlet are properly connected. If the equipment is not grounded, we can no longer
warrant the electrical safety performance of the equipment.
3. Maintenance and Storage
1. Clean all glass components by wiping gently with gauze. To remove fingerprints or
oil smudges, wipe with gauze slightly moistened with a mixture of ether (70%) and
alcohol (30%).
★Since solvents such as ether and alcohol are highly flammable, they must be
handled carefully. Be sure to keep these chemicals away from open flames or
potential source of electrical sparks---for example, electrical equipment that is being
switched on or off. Also remember to always use these chemicals only in a
well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other
than the glass components. To clean them, use a lint-free, soft cloth slightly
moistened with a diluted neutral detergent.
3. be careful not to spill any liquid – such as a culture solution –on the unit. if you do
spill any thing, immediately set the main switch to off and unplug the power cord. then
wipe away any liquid on microscope.

5
4. Do not disassemble any part of the microscope as this could result in malfunction
or reduced performance.
5. If no objectives are mounted, be sure to cover the objective mounting threaded
positions on the revolving nosepiece to prevent any dust and spilled culture solution
from getting on the lenses inside.
6. When not using the microscope, keep it covered with a dust cover. Make sure the
lamp socket is cool before covering the microscope.
4. Safety Symbols
Symbol Explanation
Indicate that the surface becomes hot ,and should not be
touched with bare hands.
Before use, carefully read the instruction manual. incorrect use
could result in personal injury to the user and/or damage to the
equipment.
Indicate that the main switch is ON.
Indicate that the main switch in OFF.
INVERSED BIOLOGICAL MICROSCOPE
INVERSED BIOLOGICAL MICROSCOPE

6
I. Nomenclature
INVERSED BIOLOGICAL MICROSCOPE
2. Installation
2-1 Installation Diagram
The following figure shows the installation sequence of the components. The number in
the figure shows the installation sequence.
Before installing, be sure every component is clean, do not score any parts or
glass surface.
Lamp house
Filter holder
Eyepieces
Phase contrast slider
Observation tube
Illumination column
Condenser
Mechanical ruler
Auxiliary stage
Objective
Nosepiece
Main switch
Light intensity control knob
35/54 mm Petri dish holder
4x, 10x, 20x, 40x
Main frame
Terasaki holder
Fig.2
Stage

7
Keep well with the supplied S1.5 and S2 hexagon wrench. When changing the
components, you will need it again.
2-2 Installing steps
2-2-1 Installing and replacing the lamp（Fig.4）
Please use the specified halogen Lamp 6V30W.
1. Hold to the bulb ①after you wrap it with gauze or other protection materials, then
depress the plugs②into the jack④on the lamp house, ensure the filament and the
bolt③are in a same level.
④
Fig.3

8
2. Replacing the lamp when using or soon after
When using, or soon after it is turned off, the lamp, the lamp house and nearby parts
will be very hot and will cause serious burns. Please turn the main switch to “O” (off),
pull up power plug, and make sure the bulb, the lamp house and periphery are all cool.
Then, you can do your replacement.
Please insert the bulb gently, or it will be damaged by excessive extrusion.
Do not touch the Halogen bulb with your bare hands. It will shorten the service
life or cause it to burst. If you leave fingerprints on the surface carelessly, clean
it with a dry soft cloth.
2-2-2 Installing the lamp house（Fig.5）
Keep the BNC connector plugs③and the lamp house pin②in line, and keep the
bolt①and the condenser jack④in line, too. Then push the lamp house into the
illumination unit gently until they are against each other.
2-2-3 Mounting the objectives（Fig.6-7）
1. Turn the coarse adjustment knob① as Fig.6 shows until the revolving nosepiece is set
Fig.4 Fig.5

9
at its lower limit.
★For ensuring the safety of the instrument during transportation, the nosepiece is
located in the lowest position and the tension adjustment collar②is adjusted to an
appropriate tension while leaving the factory.
2. Screw the lowest magnification objective onto nosepiece from the nearside, then turn
the nosepiece clockwise, mount other objectives according the magnification sequence of
low to high.
◎Mount objectives in this way will make the change of magnification to be very easy in
using.
◎It also can install the objective through the stage opening.
★Clean the objective regularly, the objective used in the inversed microscope is very
sensitive to dust.
★Be sure to cover any unused threaded positions with the objective caps to prevent dirt
and dust from getting inside.
★When operating, use the low magnification objective （4X or 10X）to search and focus
the specimen at first, then use higher magnification objectives if necessary.
★When replacing the objectives, slowly turning the nosepiece until you hear “clicked”,
that means the objective enter into the right position—center of the light path.
Fig.6 Fig.7

10
2-2-4 Installing the stage lengthen splint and the mechanical ruler（Fig. 8）
2-2-5 Installing the stage inserted plate（Fig.9）
INVERSED BIOLOGICAL MICROSCOPE
2-2-6 Installing the eyepiece（Fig.10）
1. Remove the cap of the eyepiece tube①.
2. Insert the eyepiece②into its tube③until they are against each other.
3. screw tightly the bolt③with provided S1.5 wrench to keep the eyepiece from dropping
out.
Fig.8
◎ Stage lengthen splint can be installed at
either side of the stage to enlarge the work
surface. But you can’t install the
mechanical ruler together at the same side.
◎Generally, the mechanical ruler will be
installed at the right side for comfortable
adjustment.
1. Installing the stage lengthen splint
First, Screw the fixed bolt①on to the
splint, then mount it on to the stage from
right or left below, screwing down the bolt
until it stay hard.
2. Installing the mechanical ruler
Please install the ruler in the same way as
the stage splint.
1. When using the glass stage ①, there is no
special requirement, you just need to place
it in a plane.
2. Install the stage inserted plate on to the stage
opening.
◎Turn the disk, let the V nick face the user, so
the recognition of the objective will become
easier.
Fig.9
Fig.9

11
2-2-7 Installing the color filters（Fig.11）
INVERSED BIOLOGICAL MICROSCOPE
Fig.10
Fig.11
Let the filters cool down sufficiently before
replacing them. Take out the filter holder①and
insert the required filters②.
Push the filter down to the bottom as shown in
Fig.11 ③so that it does not tilt. If the filter is
inclined or is not pushed down to the bottom④, it
may fall off the filter holder.
◎More than one filter can be stacked in the filter
holder. You can mount as many as you like, as
long as the total thickness does not exceed
11mm.

12
2-2-8 Connecting the power cord（Fig.12,13 &14）
Fig .12
Fig .13
★Cables and cords are vulnerable when bent
or twisted. Never subject them to excessive
force.
1.Make sure that the main switch of the power supply
is set to “o”(OFF)before connecting cables.(Fig.12)
2.Connect the plug④of the illumination column firmly
to its jack⑤on the rear of the microscope.(Fig.13)
3. Connect one end of the power cord ②into its
connector ③on the rear of the microscope.(Fig.13)
4. Connect the other end of the power cord②to a wall
outlet⑥.(Fig.14)
★Do always use the supplied power cord .If lost or
damaged, select the same standard cord, please.
★Connect the power cord correctly, to ensure the
instrument is grounded.

13
2-2-9 Replacing the fuse (Fig.12-14)
3. Controls
Fig.14
★Do remember to turn the main switch①to the
state of “O” (off) before replacing the fuse, and
unplug the power cord②. Rotate the fuse kits out
of the holder by the “--”type screwdriver, replace a
new fuse, then rotate back to the holder again.
★Fuse rating: 250V，1A.
Aperture iris diaphragm
Filter holder
Fine focusing knob
Coarse focusing
Tension adjustment collar
Phase contrast slider
Optical path switching
Light Annulus Centering
Fig.15

14
4. Using the controls
4-1 Microscope Frame
4-1-1 Turning on the lamp (Fig.16）
4-1-2 Adjusting the brightness( Fig.16）
4-1-3 Adjusting the tension of the coarse
adjustment knob（Fig.17）
★Be sure to use the tension adjustment
ring to adjust the rotation tension of the①
coarse adjustment knob.
Adjustment procedure:
◎How to adjust the tight tension
Turning the tension adjustment ring with①
your finger by counter-clockwise in the figure,
the tight tension of the coarse focus knob is②
increasing；And if at the contra direction, the
tight tension will decline.
If the nosepiece dropped automatically, or the
specimen defocused soon even you focus with
the fine focus knob . It means the coarse③
focus knob is too loose, you should screw it
down at the direction shown by the arrowhead
in the Fig.18.
Fig.16
Fig.17
Connect the power supply, turn on the
main switch①(shown in Fig.16)which on
the bottom side of the base to “－”(on).
Turning the brightness adjustment knob②
clockwise, the voltage raise, and the
brightness strengthen; Whereas turning at
the contra direction, the voltage decline and
the brightness weaken.

15
4-2 stage
4-2-1 Placing the specimen（Fig.18& Fig.19）
Put the specimen in the center of the stage.
★ to obtain the best image effect, please
select the containers, such as culture dish
and culture bottle, with the bottom
thickness of 1.2mm, and the same
thickness is also required for the specimen
slide.
◎Using the Φ35mm culture dish
You can lay a Φ35mm culture dish on the
stage directly by using the standard center
board①of the stage.
◎Using the mechanical ruler
1. When using the 96bit or 24bit micro-titration
board, please fasten it tightly by the stage clips
②.
2. When fastening other model boards, please
use the following supplied brackets with
mechanical ruler:
zTerasaki bracket⑦for Terrasaki board.
zCulture dish bracket⑤forΦ35mm culture dish.
zSlide bracket⑥for slide and Φ54mm culture
dish
Turning the transverse knob③and lengthways
knob④, move the specimen to the required
position.
( Movement Range: 120（width）×78（Length）
mm)
Fig.18
Fig.19

16
4-2-2 Moving the specimen
4-3 The viewing tube
4-3-1Adjusting the diopter（Fig.20）
Turn the X-axis and Y-axis knobs of the mechanical stage or move the specimen directly
by hand.
★Be careful when changing objectives. When objectives are switched after
observing the specimen with an objective with short working distance, the
newly selected objective may interfere with the stage center plate or Petri dish
holder. Turn the coarse focusing knob slightly to lower the objective properly
for that.
1. Look into the right ocular by your right eye,
then revolving the coarse focus knob to
focus on the specimen.
2. Then use your left eye to look into the left
ocular. If the image is not sharp, just use the
diopter adjustment ring①to adjust please.
★There are ±5 diopter in the adjustment
ring. The number which the reticle on the
eyepiece holder pointed is your eye’s
diopter graduation.
1. While looking through the left eyepiece with
your left eye, turn the coarse and fine focus
adjustment knobs to bring the specimen into
focus. While looking through the right eyepiece
with your right eye, turn only the diopter
adjustment ring ①to focus on the specimen.
★There are ±5 diopter in the adjustment
ring. The number which the reticle on the
eyepiece holder pointed is your eye’s
diopter graduation.
Fig .20

17
4-3-2 Adjusting the interpupillary distance
(Fig.20-21)
4-3-3 Selecting the light path
While looking through the eyepieces, move
both eyepieces until the left and right fields
of view coincide completely.
★The reticle on the interpupillar distance
indicator③, pointed by the spot “.” ②on
the eyepiece holder, shows the scale of
the interpupillar distance.（Fig.20）
The range of the interpupillary distance：55～
75mm。
◎Used for digital photography, CCD and 135 camera.
◎Revolve two knobs in fig.22 respectively to select the light path you need.
◎when in the binocular observation, turn the knob to the gap position until you heard a
“clicked” .while using video or photography with upper or lower port, turn the upper or lower
knob until it reached the “clicked” position.
Fig.21
Upper knob
Lower knob
Fig.22

18
Light path
switching knob Light Intensity ratio Application
Both the upper and
lower knob is set to
gap position
100% for binocular observation Dark specimen observation
The upper knob is
set to“PHO”position
80% for binocular eyepieces，20% for
TV/photography
Observation of bright
specimens, photography, TV
observation
The lower knob is
set to“PHO”position
100% for TV/photography Photography, TV observation
4-4 Illumination
4-4-1 Using color filters（Fig.23）
◎using appropriate filters according to the purposes allows you to observe and
photograph specimens more effectively. Particularly, the use of the LED filter is
recommended in observation and photomicrography because it renders more neutral
colors.
◎ More than one filter can be stacked in the filter holder (filter diameter: 45mm maximum;
thickness of stacked filters: 11mm)
Fig.23

19
XDINVERSED BIOLOGICAL MICROSCOPE
-202 型倒置生物显微
4-4-2 Using the aperture diaphragm（Fig.24）
Filter Application
IF550 Monochrome contrast filter (green)
LBD Color temperature conversion filter (for observation and
photomicrography)
SIF800 Exposure time compensation in photomicrography
◎the aperture iris diaphragm determines the numerical aperture of the illumination
system in bright field observation.Only when the numerical aperture of the
objective and the illumination system being matching, you can obtain the higher
image resolution and contrast, and the increased depth of focus, too.
●Checking the aperture iris diaphragm
Remove the eyepiece when necessary (and inset the centering telescope if you
have one), then look into the eyepiece sleeve; you will see the field of view as
shown in Fig.24.now adjust the aperture iris diaphragm lever as required.
●Generally, when observing a dyed specimen, set the aperture iris diaphragm②to
70% to 80% of the N.A. of the objective①in use.However, when observing a
culture specimen, which is not dyed, set the aperture iris diaphragm lever
toward“ ” .
70-80%
30-20%
Fig.24