Stalwart STM-2035 Series User manual

Biological Microscope
Model：STM-2035 Series
Instruction Manual
STM-2035DA1
STM-2035DA2
This manual is written for biological microscope STM-2035 series. To ensure the
safety , obtain optimum performance and to familiarize
you fully with the use of this
microscope , it is recommended strongly that you study this manual thoroughly
before using the microscope and retain this manual in an easily accessible place
near the work desk for future reference.

2
User Notices STM-2035
Ⅰ. Safety Notes
1. Carefully open the box, avoid the accessories, like lens, dropping to ground and being
damaged.
2. Do keep the instrument out of direct sunlight, high temperature or humidity, dustyand
easy shaking environment. Make sure the stage is smooth, horizontal and firm enough.
3. When moving the instrument, please use two hands to grip with the two sides of the
microscope body.
4. During use, if the microscope is splashed by liquid, cut off the power at once, and wipe
away the splash.
5. When running, the lamp house and nearby parts will be very hot. Please ensure there is
enough cooling room for them.
6. Make sure the instrument is earthed, to avoid lighting strike.
7. For safety, be sure the main switch is in “O”(off) state before replacing the halogen lamp
or the fuse, then cut off the power, and do the operation after the lamp bulb and the lamp
house completely cool down.( Specified: Halogen Lamp 6V/20W or LED 3W)
8. Check the input voltage: be sure the input voltage which signed in the back of the
microscope is consistent with the power supply voltage, or it will bring a serious damage
to the instrument.
9. Warning against high temperature
10. Warning against electric shock.
11. Warning: before use, carefully read the manual .improper use could result in
personal injury to the user and/or damage to the equipment
12. When disposing the microscope, check the regulations and rules of your local
government and be sure to observe them.
Ⅱ. Maintenance
1. All the lenses have been well checked and adjusted. It is forbidden to disassemble them
yourself.
2. The nosepiece and coarse/fine focus unit have a compact and precise frame, so please
don’t disassemble them as possibly as you can.
3. Keep the instrument clean, wipe dust regularly, and be attention to avoid contaminating
the optical elements especially.

3
4. The contaminations on the prism, as finger mark and oil, could be gently wiped with a
piece of soft cloth or tissue paper, gauze which has been immersed in pure alcohol or
aether. (Note that the alcohol and ether are highly flammable, do keep them away
from the fire or potential sources of electrical sparks, and use them in a drafty
room as possible as you can.)
5. Do not attempt to use organic solvents to clean the microscope components other than
the glass components. To clean them, use a lint-free, soft cloth slightly moistened with
a diluted neutral detergent.
6. When using, if the microscope is splashed by liquid, cut off the power at once, and
wipe up the moisture.
7. Do not disassemble any parts of the microscope, which will affect the function or
decline the performance of the microscope.
8. Place the instrument in a cool, dry position. When not using the microscope, keep it
covered with a dust cover. Make sure the lamp socket is cool before covering the
microscope.

4
2.Specifications
STM-2035
2.1 Mechanical tube：160mm
2.2 CF high-contrast objectives：
Magnification
Numerical
Aperture
（NA）
Thickness of
coverslip
(mm)
Focal length
f
(mm)
Working
distance
(mm)
Type
4× 0.10 0.17 28.902 17.912 Dry
10× 0.25 0.17 16.6 5.6 Dry
40× 0.65 0.17 4.28 0.6 Dry
100× 1.25 0.17 1.82 0.14 Oil
2.3 Eyepieces：
Category Magnification Focal length f (mm) Field of view（mm）
Wide field eyepieces 10× 24.86 φ20
Plan eyepieces 16× 15.58 φ11
2.4 Total Magnification：
Eyepieces 10× 16× 10× 16× 10× 16× 10× 16×
Objectives 4× 10× 40× 100×
Total
magnification
40× 64× 100× 160× 400× 640× 1000× 1600×
2.5 Conjugate Distance：195mm
2.6 Compensation free binocular head：inclined at 30°
2.7 double layers mechanical stage：140mm×140mm
Moving Range：75mm×50mm
2.8 Coaxial coarse & fine focusing system, Adjustment Range: 20mm
Fine Division: 0.002mm
2.9 Condenser：NA＝1.25 Abbe condenser with iris Diaphragm，
Adjustment Range: 20mm
2.10 Illumination system：halogen lamp 6V/20W 或3W LED, Brightness Adjustable，Voltage
Rating: 110V or 220V

6
STM-2035 DA1 Digital Biological Microscope

7
STM-2035 DA2 Digital Biological Microscope
Viewing head ：Compensation free binocular viewing head, inclined at 30 °,interpupillar
y
distance:48-75mm。
Camera system：External digital camera system with C mount, 1.3M pixel CMOS image sensor

8
4.ASSEMBLY STM-2035
4.1 Assembly Diagram
The following figure shows the installation sequence of the components. The number in the
figure shows the assembly steps.
Before installing, be sure every components is clean, do not scratch any parts or glass
surface.
Keep well with hexagon wrench provided. When replacing the components, you will
need it again.
①
⑤
③
④
②
Plan eyepieces
Objectives
Main body
Power cord

9
NOTE:
Operation Conditions：
1．Temperature ：0℃～40 ℃,Maximum
Relative Humidity：85%.
2．High Temperature：High Temperature
and humidity will result in a mildewing,
dew and even ruinous instrument.
3．Avoid placing the instrument in a dusty
environment. When ending your
microscope operation, please cover it with
the dust cap.
4．Lay the microscope in a plan and stable
position, please.
4.2ASSEMBLY STEPS STM-2035
4.2.1 Preparation (Fig.1)
For protecting the microscope in shipping,
we took some protect measures, so you need to
finish the things listed below before
installation: ①move away the plastic cap on
the binocular viewing head; ②move away the
plastic cap on the microscope body; ③move
away the plastic envelop of the stage; ④
revolve the adjustment knob upwards ( firstly,
please revolve the leftward knob up to
loosen),remove the fixed block which used to
prevent the stage slipping.
4.2.2 Installing binocular viewing head
（Fig.2、Fig.3）
Insert the compensation free binocular
head into the microscope head, turn into the
right position, then screw down the bolt⑤to fix
it.
Left Locking knob
Fi
g
.2
⑤
Fig.3
Fig.1
①
④
②

10
4.2.3 Installing the eyepieces（Fig.4、
Fig.5）
Insert the eyepieces into the eyepiece
tube until they are against each other as
shown in Fig.5.
4.2.4 Installing objectives (Fig.6& 7）
1. Adjusting the coarse focus knob until the
support device of the mechanical stage
reaches its low limit position.
2. Screw the lowest magnification objective
into the nosepiece①from the left or the
right side, then revolve the nosepiece
clockwise and mount other objectives by
the sequence of low to high
magnification
Clean the objectives regularly, for lens
is susceptible to dust.
When operating, use 10×magnification
objective to search and focus specimen
firstly, then replace with higher
magnification objective if necessary.
When replacing the objective, slowly
turn the nosepiece until you hear
“clicked”, which means the objective
is in the required position—center of
the light path.
Fig.4
Fig.5
Fig.7
Fig.6
①

11
4.2.5 Installing the color filters (Fig.8)
1. Pressing the salient point under the condenser
bracket, turn the condenser bracket①out at the
direction of arrow in Fig.8
2．Put the required filters into the holder on the
bracket, and then turn the bracket back to the
right position.
★Baby blue and green filters are available in
standard outfit.
4.2.6 Connecting the power cord
（Fig.9、Fig.10）
1． Turn the main switch①to “O” (off) state
before connecting the power cord.
2． Insert the power plugs②into the power jack
⑤of the microscope; Make sure the connection
is well.
1. Plug the power cord③into the power supply
receptacle safely. Make sure the connection is
well.
★ Make sure to check the input voltage, see
whether or not same as the nameplate
indicates. Using the wrong input voltage
would result a short circuit or fire, and
destroy the instrument.
★Do use the supplied power cord all the time.
If it lost or damaged, select the same
standard cord, please.
4.2.7 Replacing the fuse (Fig.9&10)
Do remember to turn the main switch①on
the state of “O” (off) before replacing the fuse, and
unplug the power cord. Rotate the fuse kits ④out of
the holder by the “一”type screwdriver, replace a
new fuse, then rotate back to the holder again.
★This instrument have two Fuses, the rating is:
250V，500mA.
⑤
②
③
④
Fig.10
Fig.9
①
Fig.8
Filters

12
4.2.8 Replacing the Lamp（Fig.11, Fig.12）
1．Please set the main switch to “O” state (off)
before replacing, and make sure the bulb, the lamp
room and periphery are all cool enough to carry no
burn. Then, you can do your replacing.
2．Take up the collector holder①lightly, pull it out
by the direction which the figure shown.
3. Pull out the old bulb②,hold the new bulb after
you wrap it with gauze or other protection materials
and insert its pin as deeply as possible into the
jack in the lamp holder.
4. Insert the condenser holder back to the
microscope body, pushing down to make sure the
holder is holed stable enough.
Please insert the bulb gently, or it will be
damaged by excessive extrusion.
Do not touch the halogen bulb with bare
hands. It will shorten the service life or cause it
to burst. If you leave fingerprints on the surface
carelessly, clean it with a piece of dry soft cloth.
When replacing LED ：
1. Generally, the LED has long service life and is not
easy to damage, if unfortunately it damaged,
purchase a new one from the supplier.(Fig.14)
2. Take the base plate③down from the bottom side
of the microscope base with screw driver ,loose the
bolt ④to take the old LED off, pull out the other
end of LED wire from the breadboard⑥,replace the
old LED with a new one .
3. Put the new LED unit back onto the bracket with
the bolt④and insert the end of LED wire onto the
breadboard⑥.
4. Install the base plate③back onto the bottom side
of the microscope with original bolts.
When you take down the base plate, please do
it gently and slowly, to avoid damaging internal
electrical wires.
Fig.11
①
②
Fi
g
.12
Fi
g
.13
③
⑤
④
⑥
Fig.14
⑦

13
5. Adjustment&Operation STM-2035
5.1 Assembly Diagram（Fig.15、16、17）
Fi
g
.15
Specimen holder
Longitudinal Adjustment Knob
Diopter ring
Transversal Adjustment Knob
Right Coarse Focus Knob
Right Fine Focus Knob
Brightness Adjustment Knob
T
ension Ad
j
ustment Colla
r
Aperture diaphragm adjust lever
Condenser Focusing Knob
Left Coarse Focus Knob
Left Fine Focus
Locking Set
Fig.16
Interpupillary Distance Indicator
Fig.17

14
5.2 Operation
5.2.1 Adjusting the brightness（Fig.18）
1． Connect the power, turn on the main
switch①(shown on the figure)which on
the bottom side of the base to “－”(on).
2．Turning the brightness adjustment
knob ②clockwise, the voltage raise, and
the brightness strengthen; whereas turning
at the contra direction, the voltage decline,
and the brightness weaken.
● The brightness is affected by kinds
conditions, like the phase contrast of
the specimen, the magnification of the
objective, the adjust ability of the eyes
and so on. Too faint or too strong light
is not suitable. So, generally you should
not adjust the brightness to the highest
intensity, or it will let the lamp working
in a fully loaded state, and bring a
shorten work life.
5.2.2 Placing the specimen（Fig.19、20）
1．Place the specimen on the center of the
stage, keeping the cover slip upward, and
then nip it with the specimen holder.
2．Turn the longitudinal and transversal
adjustment knobs which on the
mechanical ruler to move the specimen
onto the required position.
★Be careful when changing the
objective. If you finish the observation
with the short working distance
objective, and want to change another
one, be careful of not letting the objective
touch the specimen.
Fig.19
Specimen
Fig.20
Cover Slip
②
①
Fig.18

15
5.2.3 Oil spacing observation
The objective labeled “Oil” is oil immersion objective (100× objective). When use the
oil-spaced lens, you need to add special oil only used for microscope between the objective and
the cover slip. You should be aware of the cleaning procedures to be used after the use of oil. Wipe
oil from the oil immersion objective, and from any other parts in need of cleaning. Clean lenses
immediately after use. Leaving oil on the lens may allow oil to seep behind and harden on the
inside of the lens
● Immersing the oil
Add oil between the objective and the specimen, note that not all kinds oil are suitable, it is a
special one, just supplied for microscope. (Cedar oil fig. 21）
● Eliminating the bleb
The bleb in the oil will result in a bad effect on the
image quality. So make sure there is no bleb when
using the oil. You also can take the following steps to avoid
this problem:
· Turn the nosepiece gently to move the oil immersion
objective several times.
· Add more immersion oil
· Wipe away the oil, and add some other oil for replacement
5.2.4 Focus（Fig.22）
Place the specimen on the center of the stage, make
sure the cover slip is upward, then, uses the 10× objective
and the 10× ocular to perform the observation.
To avoid the objective touching against the specimen, you
should rise the mechanical stage up to make the specimen
near to the objective at first, then apart them slowly to make
focus.
When focusing, please reversely turn the coarse focus
knob ①, let the specimen falling slowly, and look into
the ocular to search image at the same time. And at last,
change the fine focus knob②to sharp the image. After
done this, you could change other magnification objective
freely with no risk of damaging the specimen.
Fig.21
Note：
Only a little oil is needed. If too
much , the residual oil will seep into
the stage and the condenser and
result in a decline of the
performance。
Fig.22
①
②
③

16
● The tight tension of the coarse focus knob
has already been adjusted before leaving
factory. If loosen (e.g. the stage slip down by
its weight), please screw the intention
adjustment collar to the right position by③
the supplied spanner.
5.2.5 CondenserAdjustment（Fig.23）
Turn the condenser focus knob to move the
condenser up and down. Raise the condenser
when using the high magnification objective,
and descend it when using the low
magnification one.
 The condenser and the objective are coaxial.
It has been adjusted well before leaving
factory, so the user needn’t to adjust them by
self .
 The highest position of the condenser has
been adjusted too. It also needn’t any user’s
operation.
5.2.6 Aperture Iris Diaphragm
Adjustment（Fig.25）
Turn the aperture iris diaphragm lever to①
adjust the aperture iris diaphragm.
● If the size of the aperture diaphragm
minified, the brightness and the resolution
declined, while the contrast and the depth
of field increased; In other words, if the size
largen, the brightness and the resolution
improved, but the contrast and the depth of
field declined.
● Generally, setting the size of the condenser
aperture diaphragm at 70%～80% of the
numerical aperture, you can obtain a clear
image with enough contrast. If the open of
the aperture diaphragm is too small, the
resolution were very low, so please don’t
minify the aperture below 60% of the
Fig.23
Fig.24
Fig.25
①

17
● The numerical aperture is marked on
the objective. For example, the mark
“ 10/0.25 ” means the magnification is
10×，and the numerical aperture is
0.25.
● If you want to observe the image of
the aperture iris diaphragm, remove
one eyepiece and look through the
tube. You will see a dark circle
encroaching on the bottom of the
tube.
5.2.7 Adjusting the Interpupillary
Distance（Fig.26）
The interpupillary distance range:
55mm～75mm。
When observing with two eyes, hold on
the left and right prism holders, turn
them around the axis to adjust the
interpupillary distance until the left and
right fields of view coincide completely,
as shown in Fig.26.
5.2.8 Adjusting the diopter（Fig.27）
1．Look into the left ocular by your left eye,
then revolving the coarse focus knob to
focus on the specimen.
2．Then use your right eye to look into the
right ocular. If the image is not sharp,
just use the diopter adjustment ring①to
adjust please.
Fig.26
Fig.27
①
Fig.26
Specimen
Image of Aperture Iris Diaphragm
The right size of the aperture diaphragm

6. Outfit STM-2035
Components Specifications Standard outfit
STM-2035 DA1 STM-2035 DA2
Main Body
Main body
Double layers
mechanical stage Standard
Condenser bracket
Viewing Head
Compensation
Free Binocular
Head ，Inclined at
30°
Compensation Free
Trinocular Head ，
Inclined at 30°
Standard
1.3M Pixel CCD
Camera System
300,000 pixel CCD
Camera System
400,000 pixel CCD
Camera System
USB Cable Standard
S-Video Cable
Condenser
N=1.25 Abbe
Condenser with iris
diaphragm
Standard Standard
Nosepiece Quadruple
Quintuple Optional
Illumination
System
6V20W halogen
lamp or 3W LED
Kohler illumination
system
Spare bulb(6V20W
halogen lamp) Standard
Spare fuse, with
rating:250mA
（500mA for
110V）
Standard
Eyepieces
EW10× WF
eyepieces Standard Standard
WF16× WF
Optional
Standard Standard
Standard
Standard Standard
Standard
Standard
StandardStandard
Optional
Optional Standard
OptionalOptional
Standard
Standard
Optional

19
eyepieces
Objectives
CF
high-contrast
objectives
4×
10×
40×
100×
Plan
achromatic
objectives
Filters Baby blue
Green
Video
attachment
Photography
attachment
1×(C mount)
0.5×(C mount)
Polarization
Set
Simple Polarization
Set
Phase Contrast
Kit
Simple Phase
Contrast Kit
Sliding Phase
Contrast Kit
Turret Phase
Contrast Kit
Dark Field
Condenser
Dry dark field
condenser NA0.9
Oil dark field
condenser
NA1.25-1.36
Fluorescent
attachment
Epi-fluorescent
attachment
Pointer system LED Optical Point
System

20
7. Trouble Shooting STM-2035
7.1 Optical Part
PROBLEM REASON SOLUTION
The edge of the field
of view has shadow or
the brightness is
asymmetry
The nosepiece is not in the located
position（objective is not in the center
of the optical path）
Turn objective to the right
position(the optical light center)
The image of the filament is not in
the center
Center it
The surface of the lens is moldy or
has contaminant（include condenser,
objective, eyepiece and collector）
Clean it up
Find dust and stain in
the field of view
There are stains on the lens（include
condenser, objective, eyepiece and
collector）
Clean it up
There are stains on the specimen Clean it up
The position of the condenser is too
low
Loosen the condenser’s locking bolt,
adjust the condenser to the right
position
The image is defocus
( low resolution \
contrast)
There is no coverslip on the specimen Add coverslip
The coverslip is too thick or too thin Use the standard coverslip
( 0.17mm )
The specimen is placed inversely Reversal it back
There was oil on the dry
objective(easily happened in 40X
objective)
Clean it up
There are stains on the lens（include
includ condenser, objective, eyepiece
and collector）
Clean it up
The oil objective didn’t immerse oil Use immerse oil
There was bleb in the oil Eliminate the bleb
Use a unsuitable oil Change to the specified one
The size of the aperture diaphragm is
too big
Minify it
There are stains on the incident lens
of the binocular tube
Clean it up
The size of the aperture diaphragm is
too small
Open it up
The position of the condenser is too
low
Adjust the position

21
One side of the image
is dark
The condenser is not in the center of
the field of view\the condenser
inclines
Install the condenser again and adjust
the center carefully by the centering
bolt
The nosepiece is not in the right
position
Turning it until it reach the “clicked”
position
The specimen is floating Fix it
The image shift in
focusing
The specimen slips on the stage Fix it
The nosepiece is not in the right
position
Turn it to the “ clicked ”position
The image is a little
yellow
Not use the blue color filter Use the blue filter
The brightness is not
enough
The size of the aperture diaphragm is
too small
Adjust again
The position of the condenser is too
low
Adjust the position
There are stains on the lens（include
condenser, objective, eyepiece and
collector）
Clean it up
7.2 Mechanical Part
PROBLEM REASON SOLUTION
The image can focus
when using high
magnification
objective
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip（0.17
mm）
The objective and the
specimen touch when
change the low
magnification to the
higher magnification
The specimen is placed inversely
The coverslip is too thick
Turn inversely
Use the standard coverslip（0.17
mm）
The specimen is not
easy to move The specimen holder is not fixed Fix it
The binocular image is
not coincident The interpupillar distance is not correct Adjust it
The eyes is too tired
No diopter adjustment Adjust the diopter correctly
The brightness is not suitable Adjust the voltage of the lamp
7.3 Electric Part
PROBLEM REASON SOLUTION
The lamp can’t light
when turning on the No power Check the connection of the
power cord