Thermo Scientific 325099H11 Owner's manual

-0°C Laboratory and
3ODVPD Freezers
Installation and Operation3
325099H11 Rev. A September 2013
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IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in
damage to the unit, injury to opertaing personnel, and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect
to this manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising from or
related to the use of this manual.
© 2013 Thermo Fisher Scientific Inc. All rights reserved.

Table of Contents
Table of Contents
Introduction .............................................................................1
Safety Precautions.....................................................................2
Unpacking ................................................................................3
Temperature Monitoring..........................................................3
Installation ...............................................................................4
Location...................................................................................4
Leveling ...................................................................................4
Wiring .....................................................................................4
Drawers....................................................................................4
Door Seal.................................................................................5
Solid Doors..............................................................................5
Final Checks ............................................................................5
Control Panel ...........................................................................6
Control Panel Features.............................................................6
Display Functions ....................................................................8
Programming Functions...........................................................9
Service Parameters..................................................................10
Operation ...............................................................................11
Temperature Settings .............................................................11
Start Up .................................................................................11
Defrost ...................................................................................12
Automatic Defrost .................................................................12
Alarm Systems ........................................................................13
Operating the Alarm ..............................................................13
Local and Remote Alarms ......................................................13
Installing a Remote Alarm (Optional) ....................................14
Alarm Test .............................................................................14
Chart Recorders......................................................................16
Set Up and Operation............................................................16
Changing Chart Paper ...........................................................17
Power Supply.........................................................................17
Calibration Adjustment..........................................................18
Drawers ..................................................................................19
Removing the Drawers...........................................................19
Reinstalling the Drawers ........................................................20
Changing Drawer Position.....................................................20
Cleaning .................................................................................21
Cleaning the Drawers.............................................................21
Cleaning the Condenser.........................................................21
Troubleshooting .....................................................................22
Warranty Statement ...............................................................23

Introduction
Thermo Fisher Scientific Laboratory and Plasma Freezers 1
1 Introduction This manual provides installation and operation instructions for laboratory
freezers with preset temperature setpoints of -30ºC.
The control system, standard on all models, includes:
• Key-operated power and alarm switch
• Preset temperature setpoint
• Digital temperature display with 0.1C resolution
• Graphic temperature display
• Audible and visual power failure indicators
• Alarm silence, ringback, and automatic reset functions
• SureTempTM alarm system test
Other standard features include:
• Keyed door locks
• Remote alarm contacts
• CFC-free refrigerant
• CFC-free foamed in-place urethane insulation
• Quiet, hermetically sealed refrigeration compressors

Safety Precautions
2 Laboratory and Plasma Freezers Thermo Fisher Scientific
2 Safety Precautions In this manual and on labels attached to this product, the words
WARNING and CAUTION mean the following:
WARNING : potential danger of electric shock which, if not avoided, could
result in serious injury or death.
CAUTION : a situation which, if not avoided, may result in minor or
moderate injury, impaired performance, or damage to the equipment.
Before installing, using or maintaining this product, please be sure to read
this manual and product warning labels carefully. Failure to follow these
instructions may cause this product to malfunction, which could result in
injury or damage.
Below are important safety precautions that apply to this product:
• Use this product only in the way described in the product literature and
in this manual. Before using it, verify that this product is suitable for its
intended use. If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be
impaired.
• Do not modify system components, especially the controller. Use
Thermo Scientific exact replacement equipment or parts. Before use,
confirm that the product has not been altered in any way.
• Your unit must be properly grounded in conformity with national and
local electrical codes. Never connect the unit to overloaded power
sources.
• Disconnect the unit from all power sources before cleaning,
troubleshooting, or performing other maintenance on the product or its
controls.
WARNING: Do not store flammable materials in this unit..

Unpacking
Thermo Fisher Scientific Laboratory and Plasma Freezers 3
3 Unpacking At delivery, examine the exterior for physical damage while the carrier’s
representative is present. If exterior damage is present, carefully unpack and
inspect the unit and all accessories for damage.
If there is no exterior damage, unpack and inspect the equipment within five
days of delivery. If you find any damage, keep the packing materials and
immediately report the damage to the carrier. Do not return goods without
written authorization. When submitting a claim for shipping damage,
request that the carrier inspect the shipping container and equipment.
4 Temperature
Monitoring IMPORTANT NOTE: We recommend the use of a redundant and
independent temperature monitoring system so that the freezer can be
monitored continuously for performance commensurate with the value of
product stored.

Installation
4 Laboratory and Plasma Freezers Thermo Fisher Scientific
5 Installation
CAUTION : Improper operation of the equipment could result in
dangerous conditions. Follow all instructions and operate within design
limits noted on the dataplate.
5.1 Location Install the unit in a level area free from vibration with a minimum of 6
inches of space on the sides and rear and 12 inches at the top. Undercounter
units require 1 inch of clearance on top.
Do not position the equipment in direct sunlight or near heating diffusers,
radiators, or other sources of heat. The ambient temperature range at the
location must be 59 to 90F (15 to 32C).
5.2 Leveling The unit must be level. If the unit is out of level, you may need to shim the
corners with thin sheets of metal.
5.3 Wiring Before connecting your freezer to a power source, be sure to check the
dataplate for correct voltage. Standard NEMA plugs are provided with all
units. Wiring diagrams are attached to the back of the cabinet.
CAUTION : Connect the equipment to the correct power source. Incorrect
voltage can result in severe damage to the equipment.
WARNING : For personal safety and trouble-free operation, this unit must
be properly grounded before it is used. Failure to ground the equipment
may cause personal injury or damage to the equipment. Always conform to
the National Electrical Code and local codes. Do not connect unit to already
overloaded power lines.
Always connect the equipment to a dedicated (separate) circuit. Electrical
codes require fuse or circuit breaker protection for branch circuit
conductors. Use time delay fuses for #12 AWG circuits.
5.4 Drawers Plasma freezers come standard with drawers. Additional drawers are offered
as available options.

Installation
Thermo Fisher Scientific Laboratory and Plasma Freezers 5
5.5 Door Seal Door seal integrity is critical for freezers. A loose fitting gasket allows moist
air to be drawn into the cabinet, resulting in quicker frost buildup on the
evaporator coil, longer running time, poor temperature maintenance, and
increased operation cost.
To check the door seal, complete the following steps:
1. Open the door.
2. Insert a strip of paper (a couple of inches wide) between the door gasket
and the cabinet flange and close the door.
3. Slowly pull the paper strip from the outside. You should feel some
resistance.
4. Repeat this test at 4-inch intervals around the door. If the door does not
seal properly, replace the gasket.
5.6 Solid Doors Solid doors stay open if opened 90 degrees. Solid door spring tension cannot
be adjusted.
5.7 Final Checks Before start up, complete the following steps:
1. Make sure that the unit is free of all wood or cardboard shipping
materials, both inside and outside.
2. Verify that the unit is connected to a dedicated circuit.

Control Panel
6 Laboratory and Plasma Freezers Thermo Fisher Scientific
6 Control Panel
Figure 1. Laboratory Freezer Control Panel
6.1 Control Panel
Features
The control panel is located on the top right side of your freezer. You can
use the three pushbuttons (#5, #8, and #9 in Figure 2) to change the
temperature display (#1) or to adjust temperature and alarm setpoints. The
thermometer display (#2) provides a quick visual indicator of current
cabinet temperature and alarm conditions.
1. Main temperature display — during normal operation, shows cabinet
temperature in degrees Celsius, as measured by the sensor inside the
cabinet. You can use the buttons to display other values such as setpoints
and highest and lowest recorded temperatures. The number in the main
display flashes when the value can be modified.
2. Thermometer — shows cabinet temperature and alarm conditions.
There are 10 horizontal bars: 9 are displayed during normal operation,
the tenth (top) bar indicates a warm alarm condition. The number of
bars illuminated indicates approximate cabinet temperature. Depending
on alarm settings, 4 or 5 bars illuminated indicate that the cabinet is at
setpoint. For example, suppose that the cabinet temperature setpoint is
-20ºC and that the warm and cold alarm setpoints are -16ºC and
-24ºC. Then the number of bars illuminated indicates cabinet
temperature as shown in Table 1

Control Panel
Thermo Fisher Scientific Laboratory and Plasma Freezers 7
Table 1. Thermometer display on control panel (setpoint -30ºC)
When cabinet temperature exceeds the warm alarm setpoint, the top bar
of the thermometer flashes. When temperature is lower than the cold
alarm setpoint, the bulb flashes.When you are in programming mode
(described in Table 3) the thermometer shows the setpoint value you are
changing.
3. Power failure — illuminated when the main power supply is
interrupted. In this case the audible alarm also sounds.
4. Service required — illuminated when the controller is in service
programming mode or when simulated warm or cold alarm conditions
are failing to occur during an alarm test.
5. Increase — used to increase setpoint values in programming mode and
for various display functions.
6. Door ajar — illuminated when the freezer door is open, the alarm is
activated, and the key switch is turned to the alarm position).
7. Battery low — illuminated when the backup battery is low.
8. Decrease — pushbutton used to decrease setpoint values in
programming mode and for various display functions.
9. Scan — pushbutton used to change the main display and for various
other functions.
10. Audible alarm — illuminates during warm and cold alarm conditions.
For full descriptions of display, programming, and service functions, refer to
the following tables.
Bars displayed Temperat
ure
Bars displayed Temperature
bulb only -34ºC
(cold
alarm)
6 bars -28ºC
1 bar -33 7 bars -27
2 bars -32 8 bars -26 (warm alarm)
3 bars -31 9 bars -25
4 bars -30
(setpoint)
10 bars -24
5 bars -19

Control Panel
8 Laboratory and Plasma Freezers Thermo Fisher Scientific
6.2 Display Functions
Table 2. Control Panel Display Functions
Function Meaning Sequence Display
Normal
operation
Default display while
freezer is running
— Temperature display and control panel
thermometer icon show cabinet
temperature.
Cold excursion Show coldest cabinet
temperature since last
startup or reset
Press Display shows coldest logged temperature
while button is pressed.
Warm excursion Show warmest cabinet
temperature since last
startup or reset
Press Display shows warmest logged
temperature while button is pressed.
Mute Silence audible alarm Press (the Scan
button between
and )
Display and thermometer show cabinet
temperature, alarm icon continues to
flash.
Reset Return to default display
after
excursion or alarm
condition
Press and
simultaneously,
hold for five
seconds
Excursion values are reset; temperature
display shows cabinet temperature.
SureTemp
Alarm test
Tests alarm system by
warming/cooling probe
surface; key switch must be
in alarm mode
Press and
simultaneously
Display and thermometer icon show
simulated cabinet temperature, alarms
flash and sound as appropriate. Alarms
clear when test is completed.
Lower sensor Display lower cabinet
temperature
Press and
simultaneously
and hold
Display shows lower cabinet temperature
while buttons are held, returns to upper
cabinet temperature when buttons are
released. ERR is displayed if no lower
sensor probe is detected.

Control Panel
Thermo Fisher Scientific Laboratory and Plasma Freezers 9
6.3 Programming
Functions
You can enter programming mode by pressing and holding for 5 seconds.
Pressing repeatedly scrolls through the available setpoint functions: cold
alarm and warm alarm.
Table 3. Setpoint Programming Functions
Function Programming Sequence
Adjust temperature
control setpoint
Enter programming mode by pressing and holding for 5 seconds. On release, the
current temperature setpoint value flashes in the temperature display; use and to
adjust it. Press to confirm the new value. The display automatically returns to normal
operating mode 30 seconds after the last key entry or after scrolling through all available
functions.
Adjust cold alarm
setpoint
Enter programming mode by pressing and holding for 5 seconds. On release, the
current cold alarm setpoint value flashes in the temperature display; use and to
adjust it. The display automatically returns to normal operating mode 30 seconds after
the last key entry or after scrolling through both available functions.
Adjust warm alarm
setpoint
Enter programming mode and press repeatedly until the top of the thermometer is
illuminated. The current warm alarm setpoint value then flashes in the temperature
display; use and to adjust it. The display automatically returns to normal operating
mode 30 seconds after the last key entry or after scrolling through both available
functions.

Control Panel
10 Laboratory and Plasma Freezers Thermo Fisher Scientific
6.4 Service Parameters You can access service parameters by entering programming mode with the
controller key in the Power On position, then pressing for an additional 5
seconds. On release of the button, the display will go blank, then display
“SEr” with the service wrench icon illuminated. Then the firmware checksum
(read-only) will be displayed for about 4 seconds. Pressing repeatedly
scrolls through the available service functions. While you are in service
mode, the wrench icon is illuminated. For any flashing parameter you can
use and to adjust the value.
Table 4. Service Parameters
Parameter Display Notes
1. Offset Value in main display, single bar
illuminated in thermometer
Center air temperature calibration. Default value is 0
(maximum + or - 10.0).
2. Line voltage Err Not available for this model.
3. Network
address
nEt (2 sec.); Adr (2 sec.); then
value
Can only be modified by RS-485 communications
software.
4. Defrost
probe
temperature
dEF (2 sec.); Prb (2 sec.); then
value Display only.

Operation
Thermo Fisher Scientific Laboratory and Plasma Freezers 11
7 Operation
7.1 Temperature Settings The factory default temperature setting is -30C for automatic defrost
freezers, including plasma freezers.
To change the factory temperature settings, refer to the instructions in
Section 6.3.
7.2 Start Up To start up the freezer, complete the following steps:
1. Plug in the power cord.
2. All freezers except undercounter models have a double pole circuit
breaker switch located next to the power inlet. Make sure that is in the
ON (“1”) position.
3. Insert the silver colored key in the switch on the control panel and turn
the power on, turning the key switch to position 1. The compressor and
the evaporator fans should start immediately.
4. Rotate the power switch to the ALARM ON position when the
temperature drops below the warm alarm setpoint.
5. If desired, lock the cabinet door using the gold colored key. Place
duplicate key copies in a safe place.
All controls should now be fully operational, the alarm activated, and all
visual indicators active.
CAUTION : Do not operate strong radio emission sources such as
walkie-talkies within 3 feet of the freezer. EMI and RFI can affect the
performance of the control systems.

Defrost
12 Laboratory and Plasma Freezers Thermo Fisher Scientific
8 Defrost
8.1 Automatic Defrost The defrosting process on all -30C freezers initiates automatically in
response to a built-in timer.
All models are set for one defrost cycle every six hours. The defrost cycle is
20 minutes. The cycle terminates automatically if during defrost the
evaporator coil temperature exceeds 15ºC.

Alarm Systems
Thermo Fisher Scientific Laboratory and Plasma Freezers 13
9 Alarm Systems
9.1 Operating the Alarm The alarm system is designed to provide visual and audible warning signals
for both power failure and rise in temperature. The alarm is equipped with a
battery backup.
Default cold and warm alarm values are -40C and -20C. These values may
be adjusted, following the instructions in Section 6.3.
The alarm system is activated only when the key switch is turned to the
Alarm On position. The audible warning signal sounds when there is a
power failure or temperature alarm condition, or when the door is ajar for
more than 3 minutes.
The Mute function (pressing the button) allows you to turn off the audio
warning without turning off the visual indicators.
To turn off and reset flashing visual alarms, press and simultaneously.
There is also a ringback function after approximately 6 minutes if any alarm
condition remains active.
9.2 Local and Remote
Alarms
Freezer units can have either a factory-installed local alarm or an optional
user-installed remote alarm. Operating and testing procedures are the same
for both types of alarm.
The maximum distance between a freezer and a remote alarm depends on
the wire gauge used. Refer to Table 5 below.
Table 5. Wire Gauges and Distance to Remote Alarm
Wire Gauge Total Wire Length (feet) Distance to Alarm 1/2 Wire
Length (feet)
20 530 265
18 840 420
16 1,330 665
14 2,120 1,060
12 3,370 1,685

Alarm Systems
14 Laboratory and Plasma Freezers Thermo Fisher Scientific
9.3 Installing a Remote
Alarm (Optional)
Remote alarm terminals are located at the rear of the machine compartment.
The terminals are: Common, Open on Fail (Normally Closed), and Close
on Fail (Normally Open).
1. The remote alarm system has two keyhole slots on the back to hang the
alarm system on the wall. Insert two screws, no longer than a #12
truss-head type and spaced two inches apart, into a wall and mount the
alarm.
2. Make the following connections:
a. Connect the common terminal on the cabinet switch to the purple
wire on the alarm.
b. Connect the normally closed terminal on the cabinet to the black
(“open on fail”) wire on the alarm. This connection gives an alarm
when the switch contacts open.
3. Plug the alarm system service cord into an electrical outlet. This alarm is
designed for 115V/60 Hz, 115V/50 Hz, or 100V/50 Hz operation.
Note : If you want the alarm signal to sound when the switch contacts close,
connect the normally open terminal on the cabinet to the red/white (“close
on fail”) wire on the alarm. The purple and red/white wires must be tied
together in this application.
The wiring diagram is attached to the inside of the alarm back cover.
9.4 Alarm Test Your freezer is equipped with a SureTempTM testing system which
automatically tests the alarm probe and electronics. This may eliminate the
need for other methods of warming or cooling the probe, such as ice baths.
Theory of Operation During the alarm test, the temperature sensor is artificially heated by a
tiny, built-in thermoelectric heating unit which simulates warm conditions.
The electronic control module notes the sensor temperature changes and the
control panel displays these changes.
While this alarm testing procedure is very accurate and reliable, the
temperature of the refrigerated space does not change during the alarm test.

Alarm Systems
Thermo Fisher Scientific Laboratory and Plasma Freezers 15
Alarm Test Procedure
Note : This test automatically advances through all steps and stops.
1. Verify that the key position is in the Alarm On mode, and that the
current warm and cold alarm setpoints are within normal ranges (the
warm and cold simulations may not work if the setpoints are set to
extreme values).
2. To start the alarm test, press and simultaneously. During the test
the main display and thermometer bulb will indicate simulated (not
actual) cabinet temperature.
3. When simulated temperature exceeds the warm alarm setpoint, the
alarm sounds and the alarm icon on the control panel illuminates (#10
in Figure 2 on page 8).
4. The temperature display begins to drop. After a few seconds, the
temperature in the display is back in the operating range.
5. The alarm stops. The temperature on the display drops until the cold
alarm sounds.
6. The test is now complete but the alarm continues to sound until the
temperature on the display is back in the operating range.
If the simulated alarm conditions do not occur during the first five minutes
of the alarm test, the service (wrench) icon illuminates and the test is
terminated. You can also terminate the test immediately by turning the key
switch to the second (Power On) position. When during the alarm test, the
temperature display does not change or the service icon illuminates, check
the sensor connections.
After an alarm test has terminated, there is a 10-minute delay before the test
can be run again.

Chart Recorders
16 Laboratory and Plasma Freezers Thermo Fisher Scientific
10 Chart Recorders Panel-mounted six inch recorders are standard and factory-installed on all
models except for undercounter models, for which free-standing recorders
are provided.
Recorder operation begins when the system is powered on.
10.1 Set Up and
Operation
To prepare the recorder to function properly, complete the following steps:
1. Open the recorder door to access the recorder.
2. Connect the nine volt DC battery located at the recorder’s upper right
corner. This battery provides back-up power.
3. Install clean chart paper (refer to Section 10.2 below).
4. Close the recorder door.
Note : The recorder may not respond until the system reaches temperatures
within the recorder’s range.
Figure 2. Six Inch Chart Recorder
9 Volt Battery
Imprinting
Stylus
Hub-Nut and
Retaining Wire
Chart
Chart Buttons
Reference Mark

Chart Recorders
Thermo Fisher Scientific Laboratory and Plasma Freezers 17
Figure 3. Pressure Sensitive Chart Buttons
10.2 Changing Chart
Paper
To change the chart paper, complete the following steps:
1. Locate the pressure sensitive buttons at the front, upper left of the
recorder panel.
2. Press and hold the change chart button (#3 on the upper left of the
panel) for one second. The pen will move off the scale.
3. Unscrew the center nut, remove the old chart paper, and install new
chart paper. Carefully align the day and time with the reference mark on
the recorder panel (a small groove on the left side of the panel, shown in
Figure 2).
4. Replace the center nut and hand tighten. Press the change chart button
again (#3) to resume temperature recording.
10.3 Power Supply The recorder normally uses AC power when the system is operating. If AC
power fails, the LED indicator on the recorder flashes to alert you to a power
failure. The recorder continues sensing cabinet temperature and the chart
continues turning for approximately 24 hours with back-up power provided
by the nine-volt battery.
The LED indicator glows continuously when main power is functioning
and the battery is charged.
When the battery is low, the LED flashes to indicate that the battery needs
to be changed.
12
3
CHANGE CHART