Thermo Scientific TSX Series Owner's manual

Ultra Low
Temperature Freezers
TSX Series
Installation and Operation
327228H01 Rev. A January 2015
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www.thermoscientific.com/labwarranty

IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result
in damage to the unit, injury to operating personnel, and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with
respect to this manual. In no event shall Thermo be held liable for any damages, direct or incidental,
arising from or related to the use of this manual.
© 2015 Thermo Fisher Scientific Inc. All rights reserved.

Table of Contents
Table of Contents
Models.......................................................................................1
Safety Precautions....................................................................2
Unpacking.................................................................................4
Packing List..............................................................................5
General Recommendations.....................................................6
Temperature Monitoring...................................................... 6
General Usage...................................................................... 6
Initial Loading...................................................................... 6
Operating Standards ...............................................................7
Electrical Specifications....................................................... 7
Installation................................................................................8
Location ............................................................................... 8
Wiring .................................................................................. 8
Leveling ............................................................................... 9
Backup System (Optional)................................................... 9
Superinsulated Cabinet Construction................................... 9
Door Operation .................................................................... 9
Pressure Equalization Port ................................................. 11
Installing the Remote Alarm Connector ............................ 12
Intended Use ...................................................................... 12
Start Up...................................................................................13
Initial Start Up.................................................................... 13
Unit Name (Optional) ........................................................ 14
Performance Mode............................................................. 15
Security Mode.................................................................... 16
New User Entry.................................................................. 17
Operation................................................................................19
Operation Overview........................................................... 19
Home Screen...................................................................... 19
Using the Main Toolbar..................................................... 23
Setting Temperature Setpoints........................................... 23
Setting Preferences............................................................. 25
Managing Users ................................................................. 27
Screen Calibration.............................................................. 30
Regional Settings ............................................................... 30
System Management.......................................................... 31
User Log On/Off................................................................ 32
Health Status and Alarm Management ...............................33
Health Status Overview ..................................................... 33

Table of Contents
Alarms.................................................................................34
Temperature Sensor Screen ............................................... 35
Event Log Screen............................................................... 36
Power Systems Screen....................................................... 37
Exporting Event and Temperature Data............................. 39
Backup System (Optional) ....................................................42
CO2and LN2Precautions.................................................. 42
Installation.......................................................................... 43
Start Up.............................................................................. 44
Operation............................................................................ 44
Chart Recorders (Optional)..................................................45
Set Up and Operation......................................................... 45
Changing Chart Paper........................................................ 46
Calibration Adjustment...................................................... 46
Maintenance and Troubleshooting.......................................47
Cleaning the Condenser ..................................................... 47
Cleaning the Condenser Filter............................................ 47
Gasket Maintenance........................................................... 48
Defrosting the Freezer........................................................ 48
Alarm Battery Maintenance............................................... 48
Warranty ................................................................................49
Warranty
(International)........................................................................50
Appendix A:
Alarm Summary ....................................................................51
Appendix B:
Event Log Detail.....................................................................53

Models
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 1
1 Models
Model Size (xxx) Voltage (*)
Thermo Scientific -TSXxxx* 600 V

Safety Precautions
2 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
2 Safety Precautions In this manual, the following symbols and conventions are used:
This symbol used alone indicates important operating instructions which reduce
the risk of injury or poor performance of the unit.
CAUTION: This symbol, in the context of a CAUTION, indicates a
potentially hazardous situation which if not avoided could result in minor to
moderate injury or damage to the equipment.
WARNING: This symbol indicates potentially hazardous situations which,
if not avoided, could result in serious injury or death.
WARNING: This symbol indicates situations where dangerous voltages
exist and potential for electrical shock is present.
The snowflake symbol indicates extreme low temperatures and high risk of
frostbite. Do not touch bare metal or samples with unprotected body parts.
This symbol indicates a need to use gloves during the indicated procedures. If
performing decontamination procedures, use chemically resistant gloves. Use
insulated gloves for handling samples and when using liquid nitrogen.
Before installing, using or maintaining this product, please be sure to read this
manual and product warning labels carefully. Failure to follow these instructions
may cause this product to malfunction, which could result in injury or damage.

Safety Precautions
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 3
Below are important safety precautions that apply to this product:
Use this product only in the way described in the product literature and in this
manual. Before using it, verify that this product is suitable for its intended use. If
this equipment is used in a manner not specified by the manufacturer, the
protection provided by the equipment may be impaired.
Do not modify system components, especially the controller. Use OEM exact
replacement equipment or parts. Before use, confirm that the product has not
been altered in any way.
WARNING: Your unit must be properly grounded in conformity with
national and local electrical codes. Never connect the unit to overloaded
power sources.
WARNING: Disconnect the unit from all power sources before cleaning,
troubleshooting, or performing other maintenance on the product or its
controls.
WARNING: "Caution, risk of fire".This unit is charged with hydrocarbon
refrigerants.

Unpacking
4 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
3 Unpacking At delivery, examine the exterior for physical damage while the carrier’s
representative is present. If exterior damage is present, carefully unpack and
inspect the unit and all accessories for damage.
If there is no exterior damage, unpack and inspect the equipment within five days
of delivery. If you find any damage, keep the packing materials and immediately
report the damage to the carrier. Do not return goods to the manufacturer
without written authorization. When submitting a claim for shipping damage,
request that the carrier inspect the shipping container and equipment.

Packing List
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 5
4 Packing List Inside the freezer cabinet is a bag containing:
• This manual
• A CD with user’s manuals, including translated versions
• Certificates of conformance and calibration
• A remote alarm contact connector
• Posts for rear spacing
• A freezer key.
If you have ordered a field-installed chart recorder, the bag will also contain:
• Recorder installation instructions
• Extra paper
If you have ordered a backup system, the cabinet will also contain:
• A hose assembly
• English and metric connectors
If specified on the order, the bag may also include:
• A QC temperature graph and test log
• Calibration information
If you have ordered the Proximity Access Card Option, the cards will be in a bag
attached to the front of the freezer.

General Recommendations
6 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
5 General
Recommendations
5.1 Temperature
Monitoring
IMPORTANT NOTE We recommend the use of a redundant and
independent temperature monitoring system so that the freezer can be
monitored continuously for performance commensurate with the value of
product stored.
5.2 General Usage This refrigeration system is designed to maintain ultra-low temperatures with
safety in an ambient environment up to +32°C (90°F), only when the freezer is
used for storage.
WARNING: This unit is not a “rapid-freeze” device. Freezing large
quantities of liquid, or high-water content items, will temporarily increase
the chamber temperature and will cause the compressors to operate for a
prolonged time period.
Avoid opening the door for extended time periods since chamber temperature air
will escape rapidly. Also, keep the inner doors closed as much as possible. When
room air, which is higher in humidity, replaces chamber air, frost may develop in
the chamber more rapidly.
5.3 Initial Loading Allow the freezer to operate at the desired temperature for a minimum of 12
hours before loading
.
Load the freezer one shelf at a time, beginning with the top shelf. After loading
each shelf, allow the freezer to recover to the desired set point before loading the
next shelf. Repeat this process until the freezer is fully loaded.
Note Maximum shelf load is 100 lbs (45.4 kg ) per shelf.
CAUTION: Failure to follow these procedures or overloading the unit may
cause undue stress on the compressors or jeopardize user product safety.

Operating Standards
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 7
6 Operating
Standards
The freezers described in this manual are classified for use as stationary
equipment in a Pollution Degree 2 and Overvoltage Category II environment.
These units are designed to operate under the following environmental
conditions:
• Indoor use
• Altitude up to 2000m
• Maximum relative humidity 60% for temperatures up to 32°C (90°F)
• Main supply voltage fluctuations not to exceed 10% of the nominal
voltage.
• The ULT should not be connected to a GFCI (Ground Fault Circuit
Interrupter) protected outlet as it may be subject to nuisance tripping.
6.1 Electrical
Specifications
The last character in the model number listed on the dataplate identifies the
electrical specifications for your unit.
The voltage type is, as specified in the following table:
Table 1. Electrical Specifications
Model Voltage Frequency Current
V 230V 50Hz 4A

Installation
8 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
7 Installation
WARNING: Do not exceed the electrical rating printed on the data plate
located on the lower left side of the unit.
7.1 Location Install the unit in a level area free from vibration with a minimum of eight inches
(20 cm) of space on the top and sides, six inches (15 cm) in back. (Refer to
Section 7.3 for further instructions on leveling cabinets.) Allow enough clearance
so that door can swing open at least 85°.
The rear spacing posts provided with the freezer can be used to ensure proper
clearance. To install the spacing posts, screw them into the back in the rear deck area.
Do not position the equipment in direct sunlight or near heating diffusers,
radiators, or other sources of heat. The ambient temperature range at the location
must be 59 to 90°F (15 to 32°C).
7.2 Wiring
CAUTION: Connect the equipment to the correct power source. Incorrect
voltage can result in severe damage to the equipment.
CAUTION: For personal safety and trouble-free operation, this unit must be
properly grounded before it is used. Failure to ground the equipment may cause
personal injury or damage to the equipment. Always conform to the National
Electrical Code and local codes. Do not connect the unit to overloaded power
lines.
CAUTION: Do not position the unit in a way that impedes access to the
disconnecting device or circuit breaker in the back of the unit.
CAUTION: Always connect the freezer to a dedicated (separate) circuit. Each
freezer is equipped with a service cord and plug designed to connect it to a
power outlet which delivers the correct voltage. Supply voltage must be within
+10% or -10% of the freezer rated voltage. If cord becomes damaged , replace
with a properly rated power supply cord 3-G 1.5mm², CEE 7/7, 16A / 250 V .
CAUTION: Never cut the grounding prong from the service cord plug. If the
prong is removed, the warranty is invalidated.

Installation
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 9
7.3 Leveling Make sure that the floor is level. The unit must be level both front to back and
side to side.
7.4 Backup System
(Optional)
If you are using a CO
2
or LN
2
backup system, refer to Section 11 for installation
and operation instructions.
7.5 Superinsulated
Cabinet Construction
In all models, the cabinet walls have a vacuum insulation core encapsulated by a
sealed film laminate.
CAUTION: Never drill holes in or near the cabinet walls. Drilling could
damage the insulation and make the unit inoperable.
7.6 Door Operation Upright freezer models are equipped with an advanced assembly specifically
designed for ultra-low temperature freezers.
Features include:
• One-hand operation
• A front-accessible lock
• Hasps for a standard padlock to provide additional security
• Durable construction for reliable operation and safe product storage
• Optional controlled access to the freezer with Proximity Access cards
CAUTION: When moving the freezer, always grasp cabinet surfaces; never
pull the freezer by the latch handle.

Installation
10 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
7.6.1 Opening the Door For freezers with the Proximity Access Card option:
1. To unlock the door, pass the card in front of the freezer below the LCD
display.
2. Grasp the latch handle and pull it toward yourself until the latch
disengages from the cabinet strike.
3. Keep pulling by the latch handle to open the main door.
For freezers without the Access Card option:
1. Remove the padlock if installed.
2. Grasp the latch handle and pull it toward yourself until the latch
disengages from the cabinet strike.
3. Keep pulling by the latch handle to open the main door.
7.6.2 Opening the Door During
a Power Outage
In case of power outage, you may use a 9 volt battery to activate the proximity
card access system. To access the 9 volt terminal, a piece of plastic trim must be
removed near the user interface.
Slide the U-shaped trim piece down and gently pull it out.
Once the terminal is exposed, you can open the door by holding the 9 volt
battery against the terminal and passing a valid proximity card below the display
area.
Once the door is open, remove the battery.
7.6.3 Closing the Door Note The latch does not self-engage automatically when you close the door. You
must rotate the latch into the open position first.
1. Grasp the latch handle (preferably with your left hand) and pull it
toward yourself, rotating the latch into the open position.
2. Move the freezer door into the closed position and gently push the
handle away from you, making sure that the latch engages fully with the
cabinet strike.
3. Keep applying gentle pressure to the latch handle until the latch is
securely in closed position.
4. Insert the key and rotate counterclockwise to lock.
5. Replace the padlock as required.

Installation
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 11
7.7 Pressure
Equalization Port
When an upright ultra-low temperature freezer door is opened, room
temperature air rushes into the storage compartment. When the door is closed,
the fixed volume of air is cooled rapidly. Pressure drops below atmospheric
pressure, resulting in a substantial vacuum. Re-entry into the cabinet is
impossible until internal pressures are returned to atmospheric pressure. Without
a pressure equalization mechanism, it can take, in extreme cases, several hours
before the door can easily be reopened.
All upright models feature a port that provides vacuum relief after door openings.
The pressure equalization port is located in the door behind the eye-level panel
on the front of the freezer. Although the port is designed to self-defrost, excessive
frost accumulation on the inner door could eventually restrict air flow. Therefore
you should periodically inspect the inner door and brush away any loose frost
using a stiff nylon brush.

Installation
12 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
7.8 Installing the
Remote Alarm
Connector
The remote alarm contacts are located on the back of the freezer above and to the
left of the power switch. After installing the wiring from the remote alarm to the
connector, install the connector to the freezer microboard.
The pin configuration is shown in Figure 1 below.
Figure 1. Remote Alarm Pin Configuration
The contacts will trip in the event of a power outage, high temperature alarm or
low temperature alarm.
7.9 Intended Use The -86°C freezer (refer to Section 1 for the specific model series) described in
this manual are high performance units for professional use. These products are
intended for use as cold storage in research use and as a general purpose
laboratory freezer, storing samples or inventory at operating temperatures
between -50°C and -80°C.
It is not considered a medical device and has therefore not been registered with a
medical device regulatory body (e.g. FDA): that is, it has not been evaluated for
the storage of samples for diagnostic use or for samples to be re-introduced to the
body.
This unit is not intended for use in classified hazardous locations, nor to be used
for the storage of flammable inventory.

Start Up
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 13
8 Start Up
8.1 Initial Start Up To start up the freezer, complete the following steps:
1. Plug the freezer into the power outlet.
2. Turn the power switch in back of the freezer, on the bottom right, to the
ON position.
3. When the freezer is powered up, you will see a logo displayed on the
front screen for about 25 seconds, followed by a Regional Settings
screen:
Figure 2. Regional Settings Screen
This screen allows you to specify the preferred display language along with date,
time and temperature display preferences. If you prefer a display language other
than English, press the correct radio button. Press the °F radio button if you want
to change the display temperature from Celsius to Fahrenheit.
To set date, time, and 24hr/AM-PM display format, press each display you wish
to change. Up and down arrows will then appear which you can use to change
values.
On this and all other screens, a check mark icon will appear whenever you
change values. Always be sure to press the check mark icon to confirm
changes before navigating away from a screen.
Press the check mark icon to confirm changes. Press Next when ready.

Start Up
14 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
4. A sequence of screens then asks you to verify that:
• The freezer is properly grounded and connected to a dedicated power
source
• The unit has sufficient clearance
• The unit is level
• Ambient conditions are within the acceptable range.
If you are in doubt about whether any of the above installation requirements have
been met, refer to the details in Section 7 and verify them before completing the
startup procedure.
You will then be asked to specify unit name, performance mode, and security
mode, as explained in the following sections.
8.2 Unit Name
(Optional)
If desired, you can identify an individual freezer by specifying Unit Name on the
screen below.
Figure 3. Unit Name Screen
The Contact Us information is pre-filled with Thermo Scientific information.
To enter unit name or contact information, press the field, then use the
keyboard.
To edit unit name or contact information, press the pencil icon, then use the
keyboard to edit.

Start Up
Thermo Scientific TSX Ultra Low Temperature Freezers Installation and Operation Manual 15
The keyboard for this start up screen is English only.
• The Sym key switches to a symbol and numeric character set. To switch
back to the alpha character set, press the up arrow (upper case).
• The Clear key clears edits.
• The Back key backs up one character.
Press the check mark to confirm entry, then press Next to continue.
8.3 Performance Mode Specify Performance Mode on the screen below:
Figure 4. Performance Mode Screen
Use the radio button to change settings.
The default setting is Standard Mode. This setting can be used in most
applications. Customers that require optimum temperature uniformity or those
operating in high temperature or high humidity environments should select high
performance mode.
High Performance Mode optimizes temperature uniformity in the freezer and
should be used when validation protocols require tight temperature uniformity.
In addition this mode should be used when operating in ambient temperatures
higher than 80F (27C), humidity levels above 50% RH or dew points of 65 or
higher. Standard Mode is for customers who do not require maximum
temperature uniformity. It can save up to 15% in energy usage.
If you change settings, press the check mark icon to confirm.
Press Next to continue.

Start Up
16 Installation and Operation Manual Thermo Scientific TSX Ultra Low Temperature Freezers
8.4 Security Mode The next startup screen determines whether the freezer will run in the set point
security mode. Choices are Secured and Full Access.
Figure 5. Security Mode Screen
The default setting is Full Access Mode, in which all users have access to all
functions, including changing freezer set points.
In Secured mode, administrators and users have access to functions according to
the table below.
Table 2. Administrator and User Privileges
Function Administrator User
Acknowledge alarms X X
Change setpoints X
Add / delete users X
Download data X
Change freezer
settings
X
Note that if you choose to run in Full Access mode and if you have the Proximity
Access Card option installed, you will need to activate the cards after start up.
Refer to Section 9.6 for details.
You can choose Secured mode to enable set point security. In Secured mode, only
an administrator can change freezer settings.