Thermoline Scientific Equipment TI-150F User manual

LABORATORY INCUBATORS
MODELS INCLUDE:
TI-150F TI-150G
TI-250F TI-250G
TI-350F
TI-500F
WITH EUROTHERM 3216 CONTROL
& MSP MULTI SET POINT
Thermoline Scientific Equipment Pty. Ltd.
T/A Thermoline Scientific ABN 80 000 859 129
10-12 Ross Place. Wetherill Park. N.S.W. 2164. Australia.
P.O. Box 6862, Wetherill Park Delivery Centre, N.S.W. 1851. Australia.
Phone: [phone]. International: 61 2 9604 3911.
Fax [phone]. International: 61 2 9725 1706.
Email: [e-mail] Web: www.thermoline.com.au

P a g e | 2
17/03/11
IMPORTANT INFORMATION
This manual contains operating and safety information. The operator must read and
understand the contents of this manual prior to using this equipment. Incorrect
operation may cause harm or damage the severity is classified by the following Alert
Boxes. Ensure that this manual is saved for future reference:
Warning alerts apply when there
is a Possibility of personal injury.
Caution alerts apply when there
is a Possibility of damage to the
Equipment .
To maintain safe operation of this equipment be aware of the ALERT BOXES
located throughout these operating instructions.
CONTENTS
SAFETY INFORMATION
2
ALERT BOXES
2
INTRODUCTION
3
THE MANUAL
3
UNPACKING
3
LOCATION
3
INTENDED USE
3
FUNCTION
4
PRINCIPLE OF OPERATION
4
PERFORMANCE
4
C-TICK
5
INSTALLATION
5
MAINTENANCE
5
TECHNICAL ASSISTANCE
5
EXPLANATION OF CONTROLS
6
MAIN SWITCH
6
CONTROLS
6
ELECTRICAL
7
OVER TEMPERATURE INDICATION
7
OPERATING THE EQUIPMENT
7
OPERATING GUIDELINES FOR 3216
TIMER/PROGRAMMER
8
CHANGING SET TEMPERATURE
8
OPERATING THE 3216 PROGRAMMER
9 TO 12
CALIBRATION
13 & 14
WARRANTY POLICY
15

P a g e | 3
17/03/11
INTRODUCTION
Thank you for selecting this product from the large range of products manufactured in
Australia by Thermoline Scientific.
The materials incorporated in this design have been carefully selected for their
durability, technical performance, and practicability, the resultant product is one we
are proud of.
THE MANUAL
Please familiarise yourself with the information contained in this manual before using
the equipment.
Ensure that the manual is kept safe but accessible for current and future operators to
refer to.
UNPACKING
Remove the equipment from the packing material, check that it is complete.
Assemble the components and inspect for damage.
Retain the packaging materials until the equipment has been thoroughly tested.
Notify the detail of any damage without delay to your supplier, or Thermoline
Scientific.
LOCATION
Place the equipment in a well-ventilated area on a firm surface. Confirm that the
power supply is suitable for this equipment. Refer to the equipment rating plate for
details. To control temperature effectively, the equipment should be located in a
stable ambient.
WARNING: Ensure that the electrical
outlet is earthed and is of the correct
voltage and is capable of carrying the
stated current load.
INTENDED USE
This equipment is used to provide a mild temperature controlled environment for use
in microbiology, immunology, culture departments, stability storage of veterinary
samples, education and many more.

P a g e | 4
17/03/11
CAUTION: The range of equipment
described here are not to be used in
conjunction with large quantities of
water. The introduction of water and the
resultant vapour could result in
premature component failure.
WARNING: Do not use flammable
solvents or combustible materials as fire
or explosion may occur. This equipment
uses components which may be a
source of ignition.
FUNCTION
To provide an accurate controlled temperature between ambient +5C to +60C.
PRINCIPLE OF OPERATION
The range of Laboratory Incubators are ideally suited for laboratory bench top
applications or floor.
The incubator body is insulated to minimise heat loss. The outer door which is also
insulated closes on a magnetic gasket.
An Inner Clear Door for viewing products under evaluation whilst not disturbing the
conditions, is standard in each model.
Removable chrome plated open wire shelves are supplied with additional shelf
spacing.
The desired operating temperature is set at the temperature controller. A signal from
the temperature sensor located inside at the stainless steel liner wall is monitored at
the controller. The resultant calculated pulsed output is directed to a solid state relay
which switches power to the heating elements which are bonded to the exterior of the
liner.
The warmed walls of the incubator, gently and uniformly heat the air. The amount of
energy is determined by the sensor.
Air movement is either gravity ‘G’ convection, Models: TI-150G and TI-250G or Fan
‘F’ Forced, Models: TI-150F, TI-250F, TI-350F and TI-500F.
PERFORMANCE
The operating range for this equipment is ambient +5C to +60C with a control
accuracy of 0.1C.
MAXIMUM TEMPERATURE
For this product is 60C.

P a g e | 5
17/03/11
C-TICK
The equipment referred to here complies with requirement of AS/NZS 1044:1995
electromagnetic interference (EMI).
INSTALLATION (Refer to Unpacking Page 2)
Place the equipment in a well-ventilated area, on a firm level surface and ensure that
a suitable power supply is available. Allow sufficient room for the door to open fully.
WARNING: Ensure that the electrical
outlet is earthed and is of the correct
voltage and is capable of carrying the
equipment stated current load (AMPS).
The models TI-150 (Gravity) or (Fan Forced) are suitable for bench top use, where
as models TI-250G and F, TI-350F and TI-500F are better suited for floor standing
due to their height and weight.
CAUTION:
THE STANDARD Range of incubators
are not suitable for stacking.
MAINTENANCE
The Thermoline range of laboratory incubators require no routine maintenance other
than normal levels of cleanliness.
The external surface powder coated steel and the inner surface stainless steel may
be wiped clean using a damp, soft cloth.
CARING FOR STAINLESS STEEL
Stainless steel is under most conditions extremely resistant to corrosion. This is in
part due to the addition of chromium and nickel to the steel and the formation of a
durable chromium oxide at the surface during the manufacturing process. There are
several chemicals which will attack the surface of stainless steel, plus the lack of
oxygen at the surface will cause rusting, corrosion and pitting. Note: Deionised water
is particularly corrosive, to minimise, maintain a Neutral pH (7-9).
Always have in place a program of regular cleaning using a soft damp cloth with a
mild solution of soap and water and allow to dry.
TECHNICAL ASSISTANCE
If you require additional operational or technical information regarding this equipment
please contact:
Thermoline Scientific
Customer Service Division
Telephone: [phone]
Facsimile: 61 2 9725 1706
Email: [e-mail]

P a g e | 6
17/03/11
EXPLANATION OF CONTROLS
The controls are located in a control enclosure above the door.
MAIN SWITCH
This is a combination power switch and circuit breaker, when pressed this switch
allows power to be supplied to the controls and circulating fan if applicable.
Incorporated in the face of the switch is a ’MAINS ON’ indicating light.
CAUTION: The equipment is fitted with a combination circuit
breaker, mains switch. If the circuit breaker continues to trip, it
may indicate an electrical fault exists. Switch Off the power and
consult a qualified technician.
TEMPERATURE CONTROLLER / SENSOR
This is a Eurotherm 3216 PID control with digital temperature indication of both the
set value and the operating value to 0.1°C.
Engineering parameters are configured to provide stable temperature control with
minimum over shoot and quick recovery times.
Sensor type is a DIN standard platinum resistance bulb, RTD Pt100.
Maximum recommended temperature is 60°C.
OPERATING
VALUE
SCROLL
BUTTON
SET VALUE
(SP)
MAINS SWITCH
(CIRCUIT
BREAKER)
DOWN
ARROW
N
UP
ARROW
HOME
BUTTON
OVER
TEMP
LIGHT

P a g e | 7
17/03/11
ELECTRICAL - POWER LEAD
A removable mains power lead with a three pin plug and right angle female IEC
socket is supplied for connection between the male IEC socket located at the
incubator (top left side) and a ten (10 Amp General Purpose Outlet (G.P.O.)
OVER TEMPERATURE INDICATION
All Eurotherm 3216 controllers are fitted with an automatic over temperature alarm. In
the event of a malfunction of the Thermoline product, the alarm will switch off all
forms of heating if the actual temperature exceeds the set temperature by 2.0°C. This
will be indicated by the red “over temp” indicator and a scrolling message of ‘Over
Temperature’ on the bottom display of the controller.
OPERATION
WARNING: 1. To avoid injury do not use flammable solvents in this
cabinet, as fire or explosion may result. This cabinet contains
components that may ignite such materials.
WARNING: 2. Do not introduce water into this chamber as the
excessive vapour may result in premature component failure.
OPERATING THE INCUBATOR
1) Ensure that the incubator is located as previously described, refer to the
index, LOCATION, Page 2.
2) Position the mains switch to the ON position (reset) and immediately the
circulating fan, if fitted, will start and the controller will go through a short
initiation period, the two displays will then indicate the operating value at the
top and the set value at the lower display.
3) The temperature controller is a Eurotherm 3216 microprocessor based
instrument which has been factory configured for range, sensor type, and
engineering parameters for optimum control.
Limited access to the control is available to the operator, refer to the index,
CALIBRATION.

P a g e | 8
17/03/11
Operating Guidelines for a Eurotherm 3216 Timer/Programmer.
The Eurotherm 3216 digital temperature control can be used either as a simple set
point control, where the operator sets the temperature and the incubator controls at
that temperature. Or it can be operated as a program control where the temperature
ramps from one temperature at a set rate to a second temperature, and then dwells
at that temperature for a set period of time. This ramp/dwell program has 4
ramp/dwell segments.
CHANGING THE SET TEMPERATURE:
When operating at one set temperature, simply use the “raise” or “lower” keys
to increase or decrease the set temperature.
Here we have changed the set temperature from 37ºC to 44ºC. The output of
the temperature control will automatically adjust the temperature of the
incubator to the new value.

P a g e | 9
17/03/11
OPERATING THE 3216 PROGRAM:
The multiple set point program is made up of 4 ramp/dwell segments of “TGSP 1 - 4”
(Target set point), “RMP 1- 4” (Ramp to target temperature in ºC/hour), “DWEL 1 –4”
(Dwell at target temperature for “x” hours). Once the program is complete it can be
repeated up to 100 times.
For example:
Start at ambient and ramp to 35ºC as quickly as possible (TGSP.1 = 35ºC,
RMP.1 = OFF).
Dwell @ 35ºC for 5 hours (DWEL.1 = 5).
Ramp from 35ºC to 40ºC in one hour (TGSP.2 = 40ºC, RMP.2 = 5ºC/hr).
Dwell at 40ºC for 5 hours (DWEL.2 = 5).
Ramp from 40ºC to 45ºC in one hour (TGSP.3 = 45ºC, RMP.3 = 5ºC/hr).
Dwell at 45ºC for 5 hours (DWEL.3 = 5).
Ramp from 45ºC to 50ºC in one hour (TGSP.4 = 50ºC, RMP.4 = 5ºC/hr).
Dwell at 5ºC for 5 hours (DWEL.4 = 5).
Program turns off and temperature returns to the original set temperature (no
repeat cycles).
By using the “Scroll” key the operator can scroll through a list of
parameters in order to edit a program as described above. There are 15 parameters
in total.
The program would be entered as follows:
1. Use the “Scroll” key to step through each parameter. The first screen
will show “tsp.1” parameter (Target set point 1),

P a g e | 10
17/03/11
2. Use the “Scroll” key to select in turn the “tsp.1” parameter (Target set point 1),
the “RMP.1” parameter (Ramp rate 1), and the “DWEL.1” parameter (Dwell time
1). Use the raise or lower keys to set the parameters shown below.
TSP.1 (Target set point 1)
RMP.1 (Ramp rate 1)
Note with RMP.1 = OFF the temperature will rise
from ambient to 35ºC as quickly as possible.
DWEL.1 (Dwell time 1)
3. As for segment 1 described above use the “Scroll” key to select in turn the
“tsp.2” parameter (Target set point 2), the “RMP.2” parameter (Ramp rate 2),
and the “DWEL.2” parameter (Dwell time 2). Use the raise or lower keys to set
the parameters shown below.

P a g e | 11
17/03/11
Note that in this instance “RMP.2” is set at 5.0ºC/hour so that the program will
ramp from 35ºC to 40ºC in 1 hour, then dwell there for 5 hours.
4. As for segment 2 described above use the “Scroll” key to select in turn the
“tsp.3” parameter (Target set point 3), the “RMP.3” parameter (Ramp rate 3),
and the “DWEL.3” parameter (Dwell time 3). Use the raise or lower keys to set
the parameters shown below.
Note that in this instance “RMP.3” is set at 5.0ºC/hour so that the program will
ramp from 40ºC to 45ºC in 1 hour, then dwell there for 5 hours.
5. As for segment 3 described above use the “Scroll” key to select in turn the
“tsp.4” parameter (Target set point 4), the “RMP.4” parameter (Ramp rate 4),
and the “DWEL.4” parameter (Dwell time 4). Use the raise or lower keys to set
the parameters shown below.
Note that in this instance “RMP.4” is set at 5.0ºC/hour so that the program will
ramp from 45ºC to 50ºC in 1 hour, then dwell there for 5 hours.
6. Use the “Scroll” key to select the last parameter, which is “P.CYCL” or
program cycle. This parameter determines how many times the program will
be repeated. If this is left at “1”, there will be no “repeats” and the temperature
will return to the original set temperature. Unless the incubator has a cooling

P a g e | 12
17/03/11
function, the cool time will depend on the size of the incubator and ambient
conditions.
7. Now that the program has been entered use the “Scroll” key to run the
program:
8. After “P.CYCL” press the “Scroll” key again to step through to the next
parameter which would be “res t.stat”.
This symbol refers to “Timer status” “reset”, which means
that the program (or timer), is in the reset mode and is not running. The status
can be “Res”, “Run” or “Hold”, leave the program in reset mode until after
entering the program.
With the display showing “T.STAT” “Res”, use the “Raise” key to run the
program:
9. You will note that the screen displays “RUN” at the bottom whenever the
program is running.
10. Note that at any time during the program the segment can be held by
changing from “RUN” mode to “HOLD”

P a g e | 13
17/03/11
11. Use the “Home” key to return to the original display:
SENSOR CORRECTION
There are a number of factors that will affect the accuracy of the temperature
displayed in relation to the actual temperature inside the incubator, these include the
following:
Loading of the incubator. (Product load inside the incubator may affect
the air flow across the temperature sensor).
Incubator temperature. (At higher temperatures the heat loss from the
incubator will be greater).
Sensor location. (The temperature sensor can never be placed in the
centre of the incubator because it could get damaged).
Because of the above factors, it will be common to have an error between the
temperature displayed and temperature measured at the centre of the workspace.
For this reason the digital temperature controls have a parameter that can be used to
correct the temperature displayed, called the “PV Offset”.
In simple terms this “PV Offset” adds or subtracts a correction value to the displayed
temperature to make it read the correct temperature. The “PV Offset” correction
parameter can be accessed by means of the following procedure:
MEASURING EQUIPMENT
Ensure that the equipment used to verify the temperature at the desired location is
appropriate for conditions, and it has a current certificate of calibration to a traceable
source issued by a registered organisation.
ADJUSTING THE CALIBRATION OFFSET
As an example, assume that we have determined with our reference instrument that
@ 35ºC we have a +1.0ºC error, and that we should be reading 36ºC instead of
35ºC.

P a g e | 14
17/03/11
Use the “Scroll” key to step through to the last parameter. The screen will
show “PV.OFS”.
Use the up or down keys to enter an offset of +1.0ºC.
Use the “Home” key to return to the original display:
You will now notice that the +1.0ºC calibration has been added to the incubator
temperature. The instrument will then adjust the output to alter the operating
temperature.

P a g e | 15
17/03/11
PRODUCT WARRANTY
This warranty is limited to goods purchased and installed in *Australia.
Twelve (12) months full warranty on parts and labour by Thermoline Scientific
through its authorised dealers or service organisations, will repair or at its
option replace any part found to contain a manufacturing defect in material or
workmanship without charge to the purchaser for parts, service labour or any
necessary shipping or cartage costs for parts only. Replacement or repaired
parts will be warranted only the unexpired portion of the original warranty.
This warranty does not apply to damage caused by accident, misuse, fire,
flood, or acts of God. It does not apply to damage caused from failure to
properly install, operate or maintain the goods in accordance with the printed
instructions provided. To obtain prompt warranty and service, simply contact
Thermoline Scientific.
If warranty service is required, Thermoline Scientific will endeavour to have
an authorised service organisation carry out the work if there is an available
agent, however if it is decided to return the equipment to our main workshop
then it is the customers responsibility to pay the freight cost of this return,
Thermoline Scientific, after the repair, will return the goods at our cost, to the
customer.
The customer should always contact Thermoline Scientific for approval to
return and seek advice as to the best and most cost effective method of
return.
*Export Warranty: Limited to the replacement of Parts Only. Supplied Ex
Factory, Australia.
Limitation of Liability: Except to the extent not permitted by law, Thermoline
Scientific excludes all warranties, conditions and terms implied by law. If any
implied warranty, condition or term does exist, Thermoline Scientifics’ liability
for breach of that implied term is limited, at the option of Thermoline Scientific,
to replacement or repair of goods or part thereof, or payment of the cost of
repairing or replacing the goods or any part of the goods.

P a g e | 16
17/03/11
The buyer does not have, under any circumstances, any cause of action or
right to claim or recover from Thermoline Scientific for, and in respect of, any
loss or damage of any kind whatsoever, caused directly or indirectly by:
Any defect in material or workmanship of, or any other defect
whatsoever in, or unsuitability for, any purpose of the goods or any
part of the goods; or
default or negligence on the part of Thermoline Scientific or of any
employee, contractor or agent of Thermoline Scientific, or any person
for whom Thermoline Scientific had legal responsibility relating to the
supply, or otherwise concerning the goods or any legal part of the
goods.