THORLABS CC215MU User manual

Cooled Scientific
Digital Camera
User Guide
CC215MU Cooled sCMOS Scientific
Camera

Compact Scientific Digital Cameras
Table of Contents
Chapter 1 Safety...................................................................................................................................1
1.1. Precautions................................................................................................................... 1
1.2. Warning Symbol Definitions........................................................................................ 2
1.3. Product Care................................................................................................................. 3
1.3.1. Service ..................................................................................................................................3
1.3.2. Accessories and Customization............................................................................................3
Chapter 2 Description .........................................................................................................................4
2.1. Introduction................................................................................................................... 4
2.2. Receiving and Unpacking............................................................................................ 4
2.3. Supplied Equipment..................................................................................................... 5
2.3.1. Optional Items.......................................................................................................................5
Chapter 3 Setup & Installation ...........................................................................................................6
3.1. Pre-Installation - USB 3.0............................................................................................. 6
3.2. Installing the Software.................................................................................................. 6
3.2.1. Download and Run the Installation File ................................................................................6
3.2.3. Driver Selection.....................................................................................................................6
3.2.4. Installing 3rd Party Software Application Support................................................................10
3.3. Connecting the Camera.............................................................................................. 11
3.3.1. Multiple Camera Operation.................................................................................................12
3.4. Optical and Mechanical Mounting Considerations .................................................. 13
3.4.1. Using a C-Mount Lens ........................................................................................................13
3.4.2. Mounting the Camera..........................................................................................................13
3.4.3. Using the Camera with a Cage System..............................................................................14
3.5. Optical Front End........................................................................................................ 15
3.5.1. Description of Components.................................................................................................15
3.6. Optical Front End Procedures................................................................................... 16
3.6.1. Window Removal and Replacement Procedure.................................................................16
3.6.2. Re-Assembly of the Window and C-Mount Adapter and Flange Focus Adjustment..........16
3.7. Auxiliary Connector.................................................................................................... 17
3.7.1. Pin Descriptions ..................................................................................................................17
3.7.2. Signal Descriptions .............................................................................................................17
3.7.3. Auxiliary I/O Cable Requirements.......................................................................................18
Chapter 4 Operation..........................................................................................................................19
4.1. Starting the Camera.................................................................................................... 19
4.2. Camera Timing Diagrams........................................................................................... 20
4.3. Equal-Exposure Pulse (CC215MU Quantalux Only)................................................. 22
4.4. Hot Pixel Correction................................................................................................... 23
Chapter 5 CC215MU Specifications.................................................................................................24
5.1. sCMOS Sensor Specifications................................................................................... 24
5.2. Quantum Efficiency.................................................................................................... 24

Compact Scientific Digital Cameras
5.3. Imaging Specifications............................................................................................... 24
5.4. Example Frame Rates ................................................................................................ 25
5.5. CC215MU Power Consumption ................................................................................. 25
5.6. Operating/Storage Temperatures.............................................................................. 25
5.7. Sensor Temperatures in Cooling Mode .................................................................... 25
Chapter 6 Protective Glass Window................................................................................................26
6.1. Protective Glass Window Dimensions...................................................................... 26
6.2. AR-Coated Window Specifications............................................................................ 26
Chapter 7 Mechanical Drawings......................................................................................................27
Chapter 8 Troubleshooting...............................................................................................................28
8.1. Interface ...................................................................................................................... 28
8.2. Optical......................................................................................................................... 28
8.3. Operational.................................................................................................................. 29
8.4. Software...................................................................................................................... 29
Chapter 9 Maintenance .....................................................................................................................30
9.1. Cleaning Guidelines ................................................................................................... 30
Chapter 10 Regulatory ........................................................................................................................33
10.1. Certifications and Compliance –CE Declaration of Conformity ............................. 34
10.2. FCC Statement............................................................................................................ 35
10.3. Auxiliary I/O Cable Requirements ............................................................................. 35
Chapter 11 Warranty............................................................................................................................36
11.1. General Product Warranty ......................................................................................... 36
11.2. Specific Warranties and Repairs ............................................................................... 36
Chapter 12 Thorlabs Worldwide Contacts........................................................................................37

Compact Scientific Digital Cameras Chapter 1: Safety
Rev A, June 3, 2020 Page 1
Chapter 1 Safety
1.1. Precautions
Please read the instruction manual carefully before operating your Digital Camera. All statements regarding safety
and technical specifications will only apply when the unit is operated correctly.
Refer to this User’s Guide whenever the following symbols are encountered on the Digital Camera and Power
Supply.
ATTENTION
This symbol indicates that additional information is available in this user guide.
This equipment is intended for laboratory use only and is not certified for medical applications,
including but not limited to, life support situations.
Transportation and delivery may cause the Digital Camera to be warm or cool upon receipt.
Please wait for the device to reach room temperature before attempting to operate.
WARRANTY WARNING
Do not open the Digital Camera. There are no user serviceable parts in this product. Opening the
device will void your warranty. Any modification or servicing of this system by unqualified
personnel renders Thorlabs free of any liability. This device can only be returned when packed into
the complete original packaging, including all foam packing inserts. If necessary, ask for
replacement packaging. Please contact Thorlabs’ Technical Support at techsupp[email protected]
and a member of our team will be happy to assist you.
CAUTION
This product is powered directly from the USB 3.0 port of a host computer (not included), and from
an auxiliary AC-DC power supply (included). To ensure optimal performance and the safety of the
user, the host computer must use a properly grounded UL Listed or Recognized AC-DC power
supply (or comparable regulatory approvals).
Dangerous voltage exists within the power supplies. Do not tamper with or open the supplies under
any circumstances. Doing so may expose lethal voltage to personnel and will void the warranty of
the computer and the camera.

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1.2. Warning Symbol Definitions
Below is a list of warning symbols you may encounter in this manual or on your device.
Symbol
Description
Direct Current
Alternating Current
Both Direct and Alternating Current
Earth Ground Terminal
Protective Conductor Terminal
Frame or Chassis Terminal
Equipotentiality
On (Supply)
Off (Supply)
In Position of a Bi-Stable Push Control
Out Position of a Bi-Stable Push Control
Caution: Risk of Electric Shock
Caution: Hot Surface
Caution: Risk of Danger
Warning: Laser Radiation
Caution: Spinning Blades May Cause Harm

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1.3. Product Care
Handle the system with care during transportation and unpacking. Bumping or dropping the system can damage
the unit or lower system performance. If the system is mishandled during shipment, the optical components may
become misaligned, which could lead to a decrease in the image quality. If this happens, the system will need to
be realigned by qualified personnel. To ensure proper care and operation of your Cooled sCMOS Digital Camera,
please follow the handling instructions below.
•Do not store or operate in a damp, closed environment.
•Do not use solvents on or near the equipment.
•Keep away from dust, dirt, and air-borne pollutants (including cigarette smoke). The system is not designed
for outdoor use. Protect the equipment from rain, snow, and humidity.
•Do not expose to mechanical or thermal extremes. Protect the equipment from rapid variation in
temperature.
•Handle all connectors with care. Do not use unnecessary force as this may damage the connectors.
•Clean using a soft, lint free cloth. Use of isopropyl alcohol is permitted, however do not immerse in any
liquid or solvent.
•Clean any accessible optical surfaces with an appropriate optics grade tissue or cloth.
1.3.1. Service
Only trained and approved Thorlabs’ personnel should service the system. Please contact Thorlabs’ Technical
Support at [e-mail] and a member of our team will be happy to assist you.
1.3.2. Accessories and Customization
Although the system is easily adapted for custom interfaces, to achieve the listed specifications, this system should
only be used with accessories provided by Thorlabs. Any modification or servicing by unqualified personnel renders
the warranty null and void, leaving Thorlabs free of liability. Please contact Thorlabs for questions on customization.
All cameras are supplied with one USB 3.0 cable. These cables are constructed to provide reliable data
throughput at a length of 3 m. Do not replace this cable with a 3 m cable from a different vendor. The use of a
shorter cable (1 m or 2 m) might be acceptable but is not guaranteed. Never use any cable that is longer than 3 m
on a USB 3.0 system.

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Chapter 2 Description
2.1. Introduction
The CC215MU is a 2.1 MP, monochrome, USB 3.0 actively-cooled sCMOS scientific camera. It is compatible with
many related Thorlabs products, including C-mount lenses and our 60 mm cage system. It is supported by our
ThorCam camera application as well as the free Micro-Manager software. It is also supported in LabVIEW and
MATLAB through our .NET interfaces for each. This model is an extension of our Quantalux CS2100M-USB
sCMOS Compact Scientific camera.
The camera is bundled with the full complement of Thorlabs Scientific Imaging (TSI) software. For end-users, the
ThorCam software application provides full control of the camera through an intuitive user interface, and it allows
the user to acquire, pan, zoom, analyze, and save images and metadata.
For developers, TSI also offers a Software Developer’s Kit (SDK), which includes a comprehensive Application
Programming Interface (API) to streamline the integration of any TSI camera into your system. After software
installation, the SDK can be found in a sub-folder under C:\Program Files\Thorlabs. This is the same directory for
32- and 64-bit distributions.
This manual is a functional overview of the camera, and it is meant to be a companion to the Camera Quick Start
Guide, ThorCam User Guide, and the TSI Application Programming Interface Guide. All support documentation is
available at www.thorlabs.com as well as in the C:\Program Files\Thorlabs folder following the installation of
ThorCam.
2.2. Receiving and Unpacking
Your camera was thoroughly tested and carefully packed at the factory. Once the camera shipment is accepted for
delivery, the carrier assumes full responsibility for its safe arrival. Should you receive your shipment with any
damage—concealed or apparent—please contact the carrier at once. The carrier will instruct you on how to initiate
a damage claim. If a visual inspection reveals damage upon receipt, it must be noted on the freight bill or express
receipt and the notation signed by the carrier’s agent. Failure to do so can result in the carrier refusing to honor the
claim.
To return your camera to TSI for service, you must first contact your local Thorlabs office or distributor and request
a Return Material Authorization (RMA). Returns will not be accepted without an RMA. See Chapter 11, titled
“Warranty Information” for details.

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2.3. Supplied Equipment
The following is a list of equipment that is supplied with your camera
Supplied Equipment
Interface Accessory
USB 3.0 Cable 1 (3 m long)
Accessory
Hex Key –5/64" (for C-mount adapter removal)
Accessory
Lens Cap
Power Supply
5V/3A AC-DC Converter
(For TEC Cooling Power Only)
Note 1 - All cameras are supplied with one USB 3.0 cable. These cables are constructed to provide reliable data throughput at a length
of 3 m. Do not replace this cable with a 3 m cable from a different vendor. The use of a shorter cable (1 m or 2 m) may be acceptable
but is not guaranteed. Never use any cable that is longer than 3 m on a USB 3.0 system.
2.3.1. Optional Items
•Auxiliary I/O Patch cable –The auxiliary connector on the camera allows the user to access optional
camera control and internal status signals (see Section 3.7).
Order Thorlabs 8050-CAB1 and TSI-IOBOB or TSI-IOBOB2.

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Chapter 3 Setup & Installation
3.1. Pre-Installation - USB 3.0
Your camera will only operate when connected to a USB 3.0 “SS” (Super Speed) port on your PC. USB 2.0 ports
are not supported at this time. A USB 3.0 compatible port is typically blue - but not always. Look for this symbol
above the port to be sure:
If your computer does not have a USB 3.0 port, it is likely an indication that it is an older machine, and you should
consult our recommended configuration. If you must use that machine, Thorlabs recommends our PCIe USB 3.0
card (Thorlabs’ part number USB3-PCIE).
Recommended Installation Sequence
1. Run the software installer, follow the steps outlined in the next section.
2. Connect the camera, power will be applied when connected to a USB 3.0 port. See section 3.3.
3. Your PC should discover the new USB device and install the necessary drivers. This may take a minute or
so for the first instance.
4. Run the ThorCam application software.
3.2. Installing the Software
The Thorlabs camera software runs on Windows 7, 8.1, and 10 operating systems ONLY.
3.2.1. Download and Run the Installation File
3.2.2. You may download the latest version of software from our webpage ThorCam™ Software for Scientific
and Compact USB Cameras. Choose the appropriate installation format, 32-bit or 64-bit and download the file to
your host computer. You cannot install the 32-bit version on a 64-bit computer. Double-click the file to run the
installation.
Software Designer Kits (SDKs) for Windows and Linux are also available at the same download page.
3.2.3. Driver Selection
Your camera requires a driver to be installed on your computer. After the welcome screen, acceptance of the license
agreement, and entering your user information, you will be presented with a choice of drivers. Select the driver that
matches the interface on your camera, in this case USB3.0. Thorlabs offers various cameras with Gigabit Ethernet
or Camera Link interfaces. If you have more than one camera, and they have different interfaces, select all that
apply. If you’re unsure, select them all.

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Select a driver for installation by clicking on the dropdown arrow and selecting either of the two choices; “This
feature will be installed on local hard drive,” or “This feature, and all sub-features, will be installed on local hard
drive.” There are no sub-features, so both choices do the same thing.
ALL Cooled sCMOS series cameras must have the USB driver selected.
Once a driver is selected, the red X will be replaced with a hard drive icon as shown in the USB selection below.

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Click Next to proceed with the driver installation. Depending on your PC’s configuration and the drivers you selected,
you might encounter the additional Windows security dialogs below. Be sure to click “install”to complete the
operation, otherwise the installer will “roll back” and exit.
USB Driver Installation. Click “Install”. This is required for Cooled sCMOS series cameras.
Gigabit Ethernet Driver Installation. This is not required for Cooled sCMOS series cameras, but if you have
Thorlabs “GE” cameras it is recommended. If so, click “Install”.

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Once setup is complete, you may now proceed to the next section that will describe how to connect and power on
your camera. When the camera is connected and powered up, you can navigate to the ThorCam Imaging
Software as shown below:
“Start”→”All Programs”→”ThorIabs”→“ThorCam”
Or simply double-click on the ThorCam shortcut icon that was placed on your desktop during installation of the
software:

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3.2.4. Installing 3rd Party Software Application Support
After installation of Thorlabs components is complete, you have access to interfaces for select 3rd-party image
analysis packages you may already have installed on your computer, such as MATLAB®.
3rd-party application software interfaces are located in Zip files in the following directory:
C:\Program Files\Thorlabs\Scientific Imaging\Scientific Camera Support
Important: Do not develop 3rd-party solutions in this directory, as they will be deleted if you update your
ThorCam software.
To use the MATLAB and LabVIEW interfaces, extract from the Zip file and place in the appropriate directory on
your system.
Special note for users of Micro-Manager (µManager) software: support for Thorlabs scientific cameras (excluding
the CS505MUP polarization camera) is included when you run versions 1.4.14 and later. If you wish to download
or update your Micro-Manager software, please visit
https://micro-manager.org/wiki/Micro-Manager_Open_Source_Microscopy_Software
Before using your camera within Micro-Manager, first make sure that your camera is properly installed and powered-
on. You may run a session of ThorCam to confirm that the camera, software and drivers are properly installed.
Then, shut down your ThorCam session and start a Micro-Manager session.

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3.3. Connecting the Camera
Figure 1 Rear Panel Connections
To connect the camera, follow the steps below.
1. Connect the provided USB 3.0 cable1into the mating USB 3.0 port on the back of the camera. The
camera end of the cable will have two thumb screws. Make sure the connector is fully inserted, and then
tighten the thumbscrews by hand into the camera housing.
2. Connect the provided +5V / 3A power supply to the TEC Power port by threading the mating connector
onto the camera’s connector.
3. Connect the other end of the USB 3.0 cable into a USB 3.0 port (USB SS) on the host computer. Note:
The camera will turn on at this point.
4. If external I/O signaling is required connect an 8050-CAB1 cable (not included) to the I/O port. See
section 3.7.
5. Confirm that the appropriate AC Mains adapter is installed on the +5V / 3A power supply, then plug it into
a suitable AC Mains power source of 100 –240 VAC @ 50 –60 Hz.

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Figure 2 TEC Power Supply and Mains Adapters
Note 1 - All cameras are supplied with one USB 3.0 cable. These cables are constructed to provide reliable data throughput at a length of 3 m. Do not
replace this cable with a 3 m cable from a different vendor. The use of a shorter cable (1 m or 2 m) may be acceptable but is not guaranteed. Never
use any cable that is longer than 3 m on a USB 3.0 system.
3.3.1. Multiple Camera Operation
If multiple camera operation is required on the same computer, it is recommended that each camera uses a
dedicated USB 3.0 port. Do not use a USB 3.0 hub.

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3.4. Optical and Mechanical Mounting Considerations
3.4.1. Using a C-Mount Lens
For the best results, the use of a C-mount lens that matches the optical format of the sensor’s imaging area is
recommended (refer to the sensor specifications starting at Chapter 5). For more information on Thorlabs C-
Mount lenses, please visit www.thorlabs.com.
C-mount lenses are specified with a flange focal distance of 17.5 mm in free air. However, there can be
considerable variation in the flange focal distance among commercially available lenses, as well as effects due to
inline optics.
To accommodate for these variations the camera has a fixed flange distance of 18.0 mm to allow you to bring
objects at infinity in focus before the infinity stop on the lens is reached.
3.4.2. Mounting the Camera
On most commercial microscopes, the use of a suitable adapter is recommended in order to match the optical
format of the imaging area. Please note that some commercial 1X adapters may under fill the imaging area, leading
to vignetting of the image.
Six 1/4"-20 tapped holes are provided, one or two on each side of the housing. These may be used with tripods or
other optical mounting devices. These 1/4"-20 tapped holes are compatible with Thorlabs Ø1" posts. For more
information on Thorlabs Ø1" posts, please visit www.thorlabs.com.
Figure 3 Arrow Indicates One of the Six 1/4"-20 Tapped Holes on Camera
1/4"-20 Tapped Hole
(6 Places)

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3.4.3. Using the Camera with a Cage System
The cameras have 4-40 tapped holes on the front face for compatibility with Thorlabs’60 mm Cage systems.
For more information on Thorlabs’60 mm Cage system components, please visit www.thorlabs.com.
Figure 4 Camera Shown as Part of a 60 mm Thorlabs Cage System

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3.5. Optical Front End
The images below show the components located on the front end of the camera.
Figure 5 Exploded View of Optical and Mechanical Components
3.5.1. Description of Components
•C-Mount Adapter: Allows the camera to be optically coupled with standard C-mount optics, including
lenses and microscope adapters.
•Lens Cap (Not Shown): A protective cover that should always be installed during movement or shipment,
and when the camera is not in use. The lens cap threads into the C-mount adapter.
•Protective Window: Unless otherwise requested, an AR coated protective glass window is installed and
held in place by an O-ring.
oUsers may, at their discretion, remove the protective glass window, or replace it with a custom
optical filter. When using the C-mount adapter the maximum filter thickness is 0.079” (2.0 mm) with
Ø1" (25 mm).
oAll monochrome Cooled sCMOS cameras are configured with a clear, broadband, AR-coated
protective window to preserve the entire sCMOS sensor spectral response. If the image spectrum
must be limited, for example to eliminate unwanted near-infrared light, Thorlabs offers a wide range
of high-quality interference and colored-glass filters compatible with the camera.
oSee Chapter 6 for more information on the clear glass window. See Section 3.6.1 for instructions
related to removing and replacing the glass window.
Protective Window
O-Ring
C-Mount Adapter

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3.6. Optical Front End Procedures
3.6.1. Window Removal and Replacement Procedure
TSI Cooled sCMOS cameras have a protective clear glass window installed (See Section 3.5.1). The window has
a broadband anti-reflection coating that is easily scratched or smudged by oil from fingers. Use soft, clean cotton,
latex, or nitrile gloves and handle the window by the edges.
The optical front end is designed to accommodate standard Ø1" windows. Users can, at their discretion, operate
the camera without any glass installed or install custom filters in front of the sensor. The maximum filter thickness
when using the C-mount adapter is 0.079" (2.0 mm). See Chapter 6 for more information on both types of windows.
Note: If no glass is installed, dust and debris may collect on the hermetic chamber window. Care must be taken
when cleaning this window to avoid scratching or other damage to the glass. Please use every precaution to avoid
contact with the hermetic chamber window, and to keep the camera securely mounted to the optical system or
capped when not in use.
Removal of the Protective Window from the Camera
1. Using the hex key provided, remove the three socket head screws from the C-mount adapter.
2. You can now lift the C-mount adapter out of the camera housing.
3. Locate the pry slot along the perimeter of the rubber O-ring that is holding the protective window in the
adapter. Using a non-metallic tool (optic safe tweezers or equivalent) pry the O-ring out of the C-mount
adapter. Use caution to prevent the glass window from falling out of the adapter.
Figure 6 Removing the Window
4. Carefully remove the window from the C-mount adapter and place it in a safe location.
3.6.2. Re-Assembly of the Window and C-Mount Adapter and Flange Focus Adjustment
1. Replacement of the window and C-mount adapter is done in the reverse order as shown in Section 3.6.1.
Care must be taken when handling the window.
2. The O-ring used to secure the window in the adapter must be pressed firmly into the mating groove in the
adapter until both the O-ring and the window are held securely in place.
3. Clean the window prior to reinstalling the C-mount adapter into the camera.
4. Install a compatible lens. There is no further adjustment required as the C-mount adapter has a fixed flange
focal distance.
C-mount Adapter
Protective Window
O-ring

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3.7. Auxiliary Connector
The auxiliary connector on the camera allows the user to access optional camera control and internal status signals.
3.7.1. Pin Descriptions
Figure 7 Illustration of the Auxiliary Connector on the
Rear of the Camera
3.7.2. Signal Descriptions
•Pins 1, 2, 3, 10, 11: Ground. This is the electrical ground for
the camera TTL signals.
•Pin 4, STROBE_OUT (Output): STROBE_OUT is an LVTTL output that is high during the actual sensor
exposure time when in continuous, overlapped exposure mode. STROBE_OUT is typically used to
synchronize an external flash lamp or other device with the camera.
•Pin 4 EEP Alternate Function (Output –CC215MU Only): EEP - Equal-Exposure Pulse is available when
Equal-Exposure Pulse is selected in the ThorCam Settings Dialog or by other means. This signal is active
from the time when rolling reset is complete to the time when rolling readout commences. The Equal
Exposure Pulse is typically used to synchronize an external flash lamp or other device with the camera in
order to produce uniform exposures across the entire ROI. See Section 4.3.
•Pin 5 TRIGGER_IN (Input): TRIGGER_IN is an LVTTL input used to trigger exposures on the camera.
Transitions can occur from the HIGH to LOW states or LOW to HIGH, as selected using the ThorCam
interface. Default is Low to High. Minimum Trigger Pulse-width is 100 µs.
•Pin 6, LVAL_OUT (Output): LVAL_OUT refers to “Line Valid Output.” It is an active-HIGH LVTTL signal
and is asserted during the valid pixel period on each line. It returns low during the inter-line period between
each line and during the inter-frame period between each frame.
•Pin 7, OPTO I/O_OUT (Output): The OPTO I/O_OUT signal is an optically isolated output signal. The user
must provide a pull-up resistor to an external voltage source of 2.5 V to 20 V. The pull-up resistor must limit
the current into this pin to <40 mA. The default signal present on Pin 7 is the STROBE_OUT signal, which
is effectively the Trigger Out signal as well. Note: The value of the user supplied pull-up resistor affects the
rise and fall time of this output.
•Pin 8, OPTO I/O_RTN: This is the return connection for the OPTO I/O_OUT output and the OPTO I/O_IN
input connections. This must be connected to the pull-up source for OPTO I/O_OUT or the driving source
for the OPTO I/O_IN signals.
Pin Descriptions
Pin #
Signal
1
Ground
2
Ground
3
Ground
4
LVTTL STROBE_OUT / EEP
5
LVTTL TRIGGER_IN
6
LVTTL LVAL_OUT
7
OPTO I/O_OUT STROBE
8
OPTO I/O_ RTN
9
OPTO I/O_IN
10
Ground
11
Ground
12
LVTTL FVAL_OUT