Velp Scientifica TB1 User manual

Instruction Manual
TB1
Portable Turbidimeter
VELP SCIENTIFICA
Constant Commitment to Knowledge Development
10000208/A8

Velp Scientifica –TB1 Manual
Preface
This manual functions in two ways: first as a step by step guide to help you
operate the waterproof TB1 turbidimeter; second, it serves as a handy
reference guide.
It contains basic instructions that you must follow during the operation,
care and maintenance of the instrument. The safety protection provided by
this equipment may be impaired if it is used in a manner not described in
this manual. It is recommended that all operators should read this manual
prior to working with this instrument.
The manufacturer does not acknowledge any responsibility for an
improper use of the equipment, not responding to directions for use.
Note: The manufacturer, engaged in a continuous improvement of his
products reserves the right to modify any of their characteristics without
notice.
Do not dispose of this equipment as urban waste

Velp Scientifica –TB1 Manual
TABLE OF CONTENTS
1 OVERVIEW 1
1.1 Unpacking TB1Meter and Accessories 1
1.2 Display 2
1.3 Keys and Functions 3
1.4 Battery Installation 4
2 TURBIDITY CALIBRATION 5
2.1 Indexing 5
2.2 Calibration Standards 5
2.3 Preparing the standard vials 6
2.4 Calibration Procedure 6
2.5 Restoring Factory Calibration 9
3 TURBIDITY MEASUREMENT 11
3.1 General Information 11
3.2 Turbidity Measurement Procedure 11
3.2.1 Preparation of Sample Vial 12
3.2.2 Measurement Procedure 13
3.3 Single-Shot or Continuous Measurement 14
4 TROUBLESHOOTING GUIDE 16
5 ROUTINE MAINTENANCE 17
5.1 Vials –Handling, Cleaning and Care 17
6 ACCESSORIES 18
7 SPECIFICATIONS 19
8 ADDENDUM 1: TURBIDITY 20
8.1 Definition 20
8.2 Why Is It Important? 20
8.3 Measurement Principle 20
8.4 Nephelometric Turbidity Units (NTU) 21
8.5 Indexing a Vial 21
9 ADDENDUM 2: GUIDE TO GOOD MEASUREMENT TECHNIQUE 22
9.1 Maintain sample vials in good condition 22
9.2 Match Sample Vials 22
9.3 Degassing 23
9.4 Timeliness of Sample 23
9.5 Other Important Sampling Techniques 23
9.6 Calibration 24
9.7 Dilution 24
10 WARRANTY 25

Velp Scientifica –TB1 Manual
1
1 OVERVIEW
Thank you for selecting the waterproof portable TB1 turbidimeter. The TB1
allows you to measure turbidity of an aqueous sample in the field. This
instrument operates on the nephelometric principle of turbidity
measurement and is designed to meet the criteria specified in ISO 7027
and DIN 27027 standards (see 8.3 Measurement Principle on page
20).
1.1 Unpacking TB1 Meter and Accessories
The table below indicates the items that you should find in your
turbidimeter shipment.
Item
Quantity
1. Field Portable TB1 Turbidimeter with 4 “AAA” batteries
1
2. Instruction Manual
1
3. Instrument Carrying Case
1
4. Light Shield Cap
1
5. Calibration Set (0.02, 20.0,100, 800 NTU Standards)
1
6. Empty Vials
3
7. Silicone Oil
1
8. Lint free cloth
1
Remove TB1 turbidimeter from the packing carton. Carefully inspect all
items to ensure that no visible damage has occurred during shipment. If
the items you received do not match your order, please contact your
nearest distributor immediately.
WARNING: Extra care should be taken when
unpacking, opening, and handling the calibration
standards and sample vials. Surface scratches or
finger smudges on the vial surface may cause
measurement errors. Handle these items by their
caps only.
Batteries provided with the meter package are to be installed prior to use.
See Section 0 - 1.4 Battery Installation on page 4.
Figure 1 depicts the meter. The three main components of the instrument
are the sample well, the display, and the keypad. The following sections
will describe the functionality of the display and the keypad. The proper
use of the instrument and the sample well will be discussed in later
sections.

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Figure 1: Parts of TB1 Turbidimeter
1.2 Display
All the LCD segments and annunciators that can appear on the display are
shown in Figure 2. The display is used for reporting the turbidity reading
and to provide guidance for the operation of the instrument. In addition,
the display has several other annunciators that are used to communicate
error messages and provide user guidance.
CAL 13 CAL 24 mg/l
NTU
ppm
%pH
TAvg
14-segment Liquid Crystal Display
Battery Indicator
Calibration
Standards
Units of
Measurement
Figure 2: Customized LCD when switched on
Sample Vial
Sample Well
Index Mark
Liquid Crystal Display
Keypad

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1.3 Keys and Functions
The keypad has five keys: ON/OFF, CAL, ▲, ▼, and READ/ENTER.
Keys
Functions
ON/OFF
-Powers on and shuts off the meter. With the auto switch-off
feature the meter automatically shuts off 20 minutes after last
key press.
CAL
-Initiates the calibration mode of the meter. When pressed, the
meter is set to accept the first calibration standard.
-It is also used to exit the calibration mode if the user does not
want to follow the complete calibration procedure.
READ /
ENTER
-When in measurement mode, the READ/ENTER key is used to
perform a measurement.
-Single-shot measurements: When the key is pressed and
released immediately (a quick key stroke of less than 0.3
seconds), the display will blink [-Rd-] for 10 times and then
display the measured value.
-Continuous measurements: If the READ/ENTER key is pressed
and held, the instrument will perform a continuous measurement
during which the display is updated every 2 seconds. This can
be used for indexing vials. (This function is not available in
calibration mode).
After the READ/ENTER key is released, the instrument will
automatically perform a single-shot measurement.
-When in calibration mode, the READ/ENTER key is used to
confirm the measurement for the calibration standards.
▲and▼
-Active only during calibration mode. These keys are used to
select the calibration point in an incremental/ decremental
manner.
NOTE: This meter is also fitted with an auto incrementing
feature, i.e. after the successful calibration of one point it auto
selects the next calibration point, or automatically exits the
calibration mode after the fourth calibration point.

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1.4 Battery Installation
Four AAA-sized batteries are included in your meter’s packaging:
1. Use a Philips screwdriver to remove the two screws holding the
battery cover. See Figure 3.
2. Remove the battery cover.
3. Insert the batteries. Follow the diagram inside the cover for correct
polarity.
4. Replace the battery cover onto its original position using the two
screws removed earlier.
5. The meter is now ready to operate.
Philips Screws
Battery cover
Four 'AAA'-sized batteries
Figure 3: Battery Installation
NOTE:
Dispose used batteries in accordance with your local
regulations.

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2 TURBIDITY CALIBRATION
The TB1 turbidimeter was calibrated and tested prior to leaving the factory.
Therefore, it is possible to use the instrument directly out of the box.
However, re-calibration of the instrument is recommended to help you
become familiar with the operation of the instrument and the calibration
procedures. In addition, re-calibration is recommended at least once every
month for optimum accuracy.
It is recommended that you perform a full calibration using all 4 standards
provided to ensure full-range accuracy. The meter kit now includes a set of
prepared SDVB primary standards in plastic bottles which are EPA-
approved for meter calibration. This keeps the stock solutions for the 0.02,
20.0, 100 and 800 NTU standards ready to use and offers improved
precision and shelf life. A set of 4 empty vials for the standards are
included in the meter kit. Before using the meter for the first time, verify the
meter calibration by reading back the value for the prepared primary
standard at the appropriate NTU value.
However, the TB1 turbidimeter provides flexibility for you to calibrate at
selected ranges suitable for your application.
In addition, the instrument comes with a light shield cap to shield off stray
light during calibration and measurements.
2.1 Indexing
Due to the high quality of the glass vials provided, indexing is not required.
You only need to align the mark on the vial with the mark on the meter.
However, in order to achieve a better accuracy of the measurement, you
can proceed with indexing of the vials. See Section 8.5 Indexing a
Vial on page 21 for more information.
2.2 Calibration Standards
We recommend that you use the following SDVB primary standard during
calibration to achieve the accuracy stated in this manual:
CAL 1: 800 NTU Calibration Standard
CAL 2: 100 NTU Calibration Standard
CAL 3: 20.0 NTU Calibration Standard
CAL 4: 0.02 NTU Calibration Standard
It is well known that diluted Formazin is unstable. If you choose to use
Formazin to calibrate the instrument, ensure that you are using a fresh
stock suspension of Formazin to achieve the accuracy quoted for the

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instrument. The SDVB Calibration standards offered are more stable than
Formazin and have a shelf life until the expiration date on the plastic
bottle. These are primary standards that meet the regulatory requirements
for reporting for ISO and US EPA.
2.3 Preparing the standard vials
Each standard vial should be used with the same standard value (0.02,
20.0, 100, or 800 NTU) for the greatest accuracy. Follow the steps below
at each change of solution.
1. Ensure there are no scratches or marks on the glass vial. If there are
marks that could affect the turbidity measurement, select another vial.
2. Slowly and gently invert the calibration stand plastic bottle five times but
do not shake the bottle as air bubbles can affect the reading.
3. Rinse a clean vial with 3-4 ml of the calibration standard that will be
used in this vial. Invert the capped vial 5 times. Uncap and dispose of the
rinsate.
4. Repeat the rinsing steps and dispose of the rinsate.
5. Fill the vial with the primary standard solution to the fill line and cap the
vial.
6. Clean the exterior of the vial using a lint-free cloth to remove all traces
of liquid, dirt or fingerprints. Remove stubborn smudges with alcohol or a
non-abrasive glass cleaner, and silicone oil can be used to fill small
external scratches on the vial.
7. Repeat Steps 1-6 to prepare the other three standard solutions.
These standard vials can be used to check the meter calibration by
reading back the standard value daily and also be used for calibration
when needed. When the standard reading drifts more than 10% of the
standard’s NTU value, the solution should be replaced with another aliquot
from the plastic bottle following the Preparing the Standard Vials steps.

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2.4 Calibration Procedure
When calibration is required fresh standards from the plastic bottle are
recommended for the greatest accuracy. Follow the calibration sequence
from highest, 800 NTU, down to lowest standard, 0.02 NTU. See Section
2.3 “Preparing the standard vials” prior to starting the calibration.
1. Place the TB1 turbidimeter on a flat and level surface.
2. Select the calibration function of the instrument by pressing the CAL
button once. The [CAL] annunciator will blink momentarily and the
meter will prompt for the first calibration standard CAL 1 standard (800
NTU).
3. Insert the CAL 1 standard (800 NTU) into the sample well, aligning the
mark on the vial with the mark on the meter. See Figure 10
4. Press down until the vial snaps fully into the instrument.
5. Cover the vial using the light shield cover.
6. Press READ/ENTER key.
7. The [CAL 1 800 NTU] annunciator will blink for about 12 seconds.
When the instrument has completed calibration for this point, it prompts
you to insert the next calibration standard into the sample well [CAL 2
(100 NTU).
8. Repeat the calibration sequence for each calibration standard.
9. After you successfully calibrate the CAL 4 standard (0.02 NTU), the
display will show [STbY].
10.The meter is now ready to perform next measurement.
Figure 4 shows the complete calibration sequence.
NOTES:
1. If you wish to exit the calibration mode you may
do so at the end of any step by pressing the CAL
key. The meter will accept only the values
calibrated prior to exiting.
2. You can skip a calibration point by pressing ▲ or
▼ keys and move on to the next calibration point.
3. After a successful calibration of one point, it will
auto select the next calibration point. It will
automatically exit calibration mode after the fourth
calibration point.
4. If an error occurs during calibration, the display
will present an error message. The meter will
abort calibration and return to the measurement
mode without saving the last calibration value.
5. For a list of error messages, refer to Section 4:
Troubleshooting Guide.

Velp Scientifica –TB1 Manual
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CAL 1
800 NTU
NTU
CAL 1
NTU
NTU
CAL 3
CAL 3
NTU
CAL 2
NTU
CAL 2
READ
ENTER
NTU
CAL 1
CAL
Place the CAL 1
standard into the
sample well
Place the next CAL
3 standard into the
sample well
Flashes about
5 times
Flashes about 10
times or more
Flashes about 10
times or more
Flashes about 10
times or more
Flashes about 10
times or more
CAL 3
CAL 4
20.0 NTU
0.02 NTU
NTU
NTU
CAL 4
CAL 4
READ
ENTER
READ
ENTER
Place the next CAL
2 standard into the
sample well
Place the next CAL
4 standard into the
sample well
The TN-100/ T-100 meter is now ready
for measurement
CAL 2
100 NTU
READ
ENTER
Figure 4: Calibration Sequence

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2.5 Restoring Factory Calibration
The TB1 turbidimeter allows you to reset the meter back to the factory
default calibration values. This feature is extremely useful when there are
errors in calibration or when you have new calibration standards.
1. With the meter switched off, press ▲ and ON/OFF keys simultaneously
for about 2 seconds.
2. The display will flash [URST] (User-Reset) for 2 seconds and show
[No], which is the default value.
3. Use ▲ or ▼ key to select between [YES] or [No].
[YES] = To restore meter back to factory calibration values
[No] = To retain last calibrated values
4. After selecting [YES] or [No], press READ/ENTER key.
5. The display will flash [--Rd--] 10 times.
6. Once it stops flashing, it will show [--Rd--]. It is now ready to take
turbidity measurements.
Figure 5 shows the sequence for restoring factory calibration values.

Velp Scientifica –TB1 Manual
10
READ
ENTER READ
ENTER
Press and
keys simultaneously and
hold for 2 seconds before
releasing
UP ON/OFF
[-Rd-] will flash 10 times
The meter is now ready for measurement
Press or key to
select or
UP DOWN
YES No
To restore factory
calibration To retain last
calibration values
without retrieving
factory calibration.
METER OFF
OFF
ON
Figure 5: Restoring Factory Calibration

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3 TURBIDITY MEASUREMENT
3.1 General Information
The waterproof TB1 turbidimeter allows you to measure the turbidity of a
grab sample. The turbidity is reported in Nephelometric Turbidity Units
(NTU). Readings above 1000 NTU are outside the range of this
instrument.
NOTE:
Before switching on the TB1 turbidimeter, a sample
vial MUST be placed in the sample well. You can use
any of the calibration standards for this purpose.
The light shield cap provided could be used to cover
the vial during measurements.
The meter will go through a power sequence as shown in Figure 6.
CAL 13CAL 24 mg/l
NTU
ppm
%pH
TAvg
NTU
OFF
ON
"Rd" will flash 10 times
before a reading appears
(assuming you have
placed a vial into the
sample well of the meter
A reading will only appear
if you have placed a vial
or any of the calibration
standards provided into
the sample well.
Otherwise an error
message "Err 8" will
appear.
Figure 6: Power-up sequence
3.2 Turbidity Measurement Procedure
An accurate turbidity measurement depends on good measurement
techniques. Factors such as clean sample vials, positioning of vial in the
sample well, covering the vial with the light shield cap, meter calibration,
handling of meter, and others, have to be taken into consideration. Please
see Section 0: 5.1 Vials –Handling, Cleaning and Care on page 17 and
Section 9: ADDENDUM 2: Guide to Good Measurement Technique on
page 22 for more information.

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3.2.1 Preparation of Sample Vial
1. Grab liquid with the plastic bottle provided.
2. Obtain a clean and dry sample vial (Figure 7).
3. Take care to handle the sample vial by the top.
4. Rinse the vial with approximately 10 ml of the sample
water, capping the vial with the black screw cap and
gently inverting it several times. Discard the used sample
and repeat the rinsing procedure two more times.
5. Fill the rinsed vial with the remaining portion
(approximately 10 ml) of the grab sample up to the
mark indicated in the vial. Cap the vial with the
supplied black screw cap.
6. Wipe the vial with the soft, lint-free cloth supplied. Ensure that the
outside of the vial is dry, clean and free from smudges.
7. Apply a thin film of silicone oil (supplied) on the sample vial (see Figure
8). Wipe with a soft cloth to obtain an even distribution over the entire
vial’s surface.
NOTES:
1. The purpose of oiling the vial is to fill small
scratches and to mask the imperfection in the
glass.
2. Do not apply large quantity of oil as this may
collect dirt and dust.
8. You are now ready to place the vial into the meter for measurement.
Figure 8: Apply a thin film of silicone oil
Figure 7:
Sample Vial

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3.2.2 Measurement Procedure
1. Place TB1 turbidimeter on a flat and level surface.
2. Place the sample vial inside the sample well and align the vial’s mark
with the meter’s index mark. See below
Align index mark on
the sample vial with
the index mark on
the TN-100/ T-100
turbidimeter and
push till it is fully
snapped in
IMPORTANT:
Place meter on a flat
and level surface.
DO NOT hold it on
hands while operating
the meter. It may lead to
inaccurate readings
Light shield cap for
covering the vial during
measurements
Figure 8: Align sample vial's mark with meter's index mark
3. Push the vial until it is fully snapped in.
4. Cover the vial with the light shield cap.
5. Turn on the meter by pressing the
ON/OFF key.
6. After the power-sup sequence, the
display blinks [--Rd--] for about 12
seconds. See Figure 9.
7. A value will appear. Note the reading.
8. If necessary, please the second sample
vial into the sample well. Remember to
align the vial’s mark with the meter’s
index mark.
9. Press READ/ENTER key. The display
blinks [-- Rd --] and a value will appear.
Note the reading.
10.Repeat steps 2 through 9 for all of your
samples.
Flashes about 10
times
SAMPLE
Unknown
NTU
Place vial
into the
sample well
NTU
READ
ENTER
Measurement mode can start from
either situation:
1. [STbY] after calibration;
2. After powering up the meter
Figure 9: Reading turbidity
Figure 10: Value tubidity

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NOTE:
1. NEVER pour liquid directly into the sample well of
the instrument. Always use a vial. The instrument
will only accurately measure the turbidity of a
sample when vials sealed with the black caps are
used. The black cap serves as both seal and a
light shield.
2. NEVER attempt to clean the sample well. The
optics may be damaged.
3. The instrument will automatically power off 20
minutes after the last key press for battery
conservation.
3.3 Single-Shot or Continuous Measurement
You can use TB1 turbidimeter to take a single reading or perform
continuous measurement. The latter is only used for indexing the vials.
See Section 0 - 8.5 Indexing a Vial on page 21 for more information.
For single-shot measurement:
1. Make sure the meter is sitting on a flat
and level surface and is in measurement
mode. The display shows the last
measured value or [STbY] after exiting
calibration mode.
2. Place sample vial in the sample well.
3. Cover the vial with the light shield cap.
4. Press READ/ENTER key and release
immediately (<0.3 seconds). See Figure .
5. The display will blink [--Rd--] for 10 times
or more and then display the measured
value.
Press key and release
immediately
SAMPLE
Unknown
NTU Place vial
into the
sample well
NTU
READ
ENTER
Flashes about 10
times
Figure 11: Single-shot measurement

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15
For continuous measurement:
1. Make sure the meter is sitting on a flat and level surface and is in
measurement mode. The display shows the last measured value or
[STbY].
2. Place the sample vial in the sample well.
3. Press READ/ENTER key and hold. See Figure 12.
4. Wait for the reading to stabilize before rotating the sample vial.
NOTE:
During continuous measurement, the display is
updated every 2 seconds. The displayed reading
may not be the actual turbidity value. For accurate
measurement, use single-shot measurement.
5. You can rotate the sample vial for
indexing purpose. See Section 0 - 8.5
Indexing a Vial on page 21 for
more information.
6. Once you release the READ/ENTER
key, the meter will automatically
perform a single-shot measurement.
NOTES:
1. After a measurement is completed and the
display is updated, a 4-second recovery time
occurs before the meter can perform another
function. If any key is pressed during the recovery
time, the meter will perform the corresponding
action at the end of the recovery period.
2. When performing a measurement, if the meter
detects stray light exceeding the amount
equivalent to 0.02 NTU in the low range, the
measurement is immediately aborted and an error
message [ERR 8] is displayed. Ensure the vial
sits properly into the sample well. Press the
ENTER/READ key to re-do the measurement.
SAMPLE
Unknown
NTU Place vial
into the
sample well
Press and hold
NTU
READ
ENTER You can turn
the vial for
indexing
Figure 12: Continuous measurement

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4 TROUBLESHOOTING GUIDE
The TB1 turbidimeter routinely performs self-diagnostics, and will
automatically generate messages to provide you with specific diagnostic
information. These messages are for your use and do not indicate a
reduction in the performance of the instrument or a failure of any
component in the instrument, unless otherwise stated in this list.
LCD
Message
Description
Corrective Actions
ERR 1
Calibration Error. The meter is
unable to recognize the 800 NTU
Calibration Standard.
Ensure to use the correct 800
NTU calibration standard. *
ERR 2
Calibration Error. The meter is
unable to recognize the 100 NTU
Calibration Standard.
Ensure to use the correct 100
NTU calibration standard. *
ERR 3
Calibration Error. The meter is
unable to recognize the 20.0 NTU
Calibration Standard.
Ensure to use the correct 20.0
NTU calibration standard. *
ERR 4
Calibration Error. The meter is
unable to recognize the 0.02 NTU
Calibration Standard.
Ensure to use the correct 0.02
NTU calibration standard. *
ERR 5
Calibration Error. There is not
sufficient signal to achieve the
appropriate resolution in the 0-
1000NTU range.
Re-do calibration with all 4
standards provided. *
ERR 6
General Calibration Failure. There
is not sufficient signal to achieve
the appropriate resolution in the 0-
100NTU range.
Re-do calibration with all 4
standards provided. *
ERR 7
General Calibration Failure. There
is not sufficient signal to achieve
the appropriate resolution in the 0-
20 NTU range.
Re-do calibration with all 4
standards provided. *
ERR 8
Excessive stray light detected.
Ensure the vial is fully snapped
in the sample well.
Attempting a measurement when
low battery indicator is on.
Replace batteries.
ERR 9
Lamp Failure.
Return unit.
Or
When Turbidity value is above the
measurement range(>1000NTU)
Dilute sample. See Section 0 on
page 24.
Low battery indication. The
batteries need to be replaced.
Replace batteries.
* If an error message appears, take the
appropriate corrective action and re-do the
desired procedure. If the problem persists,
contact Velp or your dealer. See Sections 10
“Warranty”and on page 25.

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5 ROUTINE MAINTENANCE
The supplied carrying case is optimal for protecting the instrument. If you do
not plan on leaving the instrument in the supplied carrying case, when not in
use, ensure that the instrument has been turned off and that a clean sample
vial fitted with a black cap has been placed in the sample well.
This will ensure that a minimal amount of dust and/or debris will be able to
settle on the optics of the instrument.
5.1 Vials –Handling, Cleaning and Care
Proper measurement of the turbidity of a sample requires the use of a vial
that is free of marks, smudges, scratches and any bacterial growth.
Therefore, sample vials must be handled with absolute care to avoid
contamination or damage, which might change the optical characteristics of
the glass. Scratches, fingerprints, and water droplets on the sample vial or
inside the sample well can cause stray light interference leading to inaccurate
readings.
Cleaning the vial is accomplished by washing the interior and exterior of the
vial in a detergent solution. Once cleaned, the vial should be rinsed
thoroughly 8 to 10 times with clean distilled water to eliminate the possibility
of detergent buildup and streaking.
Vials can also be acid washed periodically and coated with a special silicone
oil to fill small scratches and mask the imperfections in the glass. Since the
silicone oil required for this application should have the same refractive
characteristics as glass, it is recommended that the oil be obtained from us.
Care should be taken not to apply excessive oil that could attract dirt or
contaminate the sample well of the meter. Once the oil has been applied to
the vial, the excess oil should be removed with a lint-free cloth. The result
should be a sample vial surface with a dry appearance, but with all
imperfections filled with oil.
Sample vials should always be handled from the top or by the cap to avoid
fingerprints or smudges. After a vial has been filled with a sample and
capped, the outside surface should be wiped with a clean, lint-free absorbent
cloth until it is dry. Cleaned and dried vials should be stored with the black
caps on. The vials can be stored in the carrying case. During normal
operation you may use any typical glass cleaner along with a lint free cloth or
tissue (Kimwipes®), to clean the outside of the vials.
Condensation may appear on the vial when your sample is very cold and the
relative air humidity is high. When this happens, the turbidity that you read
may be higher than the actual turbidity due to the light scattered by the
condensate on the vial. If you find yourself in this circumstance, you can
alleviate the problem by either coating the vial with an anti-fogging agent, or
by running warm water over the vial for a short period of time to warm the
sample prior to measurement.