ViroGates suPARnostic Quick Triage User manual

ViroGates, Denmark - [email protected] - www.virogates.com
PI 302 v1.1 MAY 2020
Quick Guide - suPARnostic® Quick Triage measurement with aLF Reader
aLF Reader set up
1. Place the aLF Reader on a stable and level surface.
2. Plug the power supply cable into the connector at the rear end of the aLF Reader.
3. If needed: Connect the DYMO LabelWritter to the aLF Reader with the supplied USB cable.
Turn on the aLF Reader
4. Turn on the aLF Reader by pressing the power button
Wait for the operating system to boot up.
The main menu will appear on the touch screen display.
Perform measurement and preparation of sample
5. To start a new test, touch “New Test” on the touch screen display.
6. Two measuring methods are available, the suPARnosticQT and the suPARnosticQT20
i. For suPARnosticQT leave the device for 20 minutes on the table for incubation before measuring in
the aLF Reader.
ii. For suPARnosticQT20 insert the device in the aLF Reader, where it will incubate for 20 minutes
before measuring automatically.
7. Using the internal 2D barcode scanner, scan the 2D barcode with the desired program, suPARnosticQT
(manual reading) or suPARnosticQT20 (automated reading), provided in the kit.
NOTE: keep the barcode in a vertical angel 10 –15 cm from the aLF Reader.
8. Test Name, Lot ID, and Sample Settings will automatically appear on the screen.

ViroGates, Denmark - [email protected] - www.virogates.com
PI 302 v1.1 MAY 2020
Sample preparation
9. Mix 100 μl of the Assay running buffer, included in the kit (A003), with 10uL fresh isolated plasma sample
in a clean tube by pipetting up and down at least 5 times or vortex briefly.
NOTE: Do not leave the mix for longer than one hour.
10. Transfer 60 μl of the mix to the suPARnostic® Quick Triage device.
11. Place the Lateral Flow cassette in the correct orientation into the drawer, as shown in the figure.
12. Insert the drawer into the device. Touch the “forward” button to proceed and confirm that the cassette
has been inserted in the correct orientation.
13. Read the 2D-barcode with the Patient ID or write the Patient ID manually.
Obtain results
14. The test results will appear on the screen after the test run has finished.
15. Touch the “Print” button to print the results with DYMO LabelWriter or touch the “Export to
USB stick” button to save the data in .csv format on USB.
16. Connect to the hospitals IT system if needed. For further information see the Qiagen User manual QLC
connect (ESLR22-DH-0001 User manual QLC connect 01) or contact Qiagen at
aLF[email protected] or call [phone].
Summary table for sample preparation
suPARnosticQT
suPARnosticQT20
1. Transfer 100 µl of running buffer to an empty tube.
2. Transfer 10 µl of plasma sample to the tube containing 100 µl of running buffer.
Vortex the mixture or use the pipet to mix up and down.
3. Transfer 60 μl of the diluted sample to the well of the suPARnostic® Quick Triage device.
4. Scan the suPARnosticQT method barcode. Incubate
the device for 20 min on the table. Insert the device
into the aLF Reader before running the method. (If the
user is NOT present during the incubation it is
recommended to use the suPARnosticQT20 method).
4. Scan the suPARnosticQT20 method barcode.
Insert the device in the aLF Reader for incubation
and touch “forward”to activate the 20 minute
incubation.
5. Press “forward”to read the device with the aLF
Reader. Use the designated batch method.
5. aLF Reader reads the device automatically after
20 min.