WPI DAM50 User manual

Instrumenting scientific ideas
WORLD
PRECISION
INSTRUMENTS
DAM50
Dierential Amplier
Serial No._____________________
www.wpiinc.com
INSTRUCTION MANUAL
092618

DAM50
World Precision Instruments i
CONTENTS
ABOUT THIS MANUAL ................................................................................................................... 1
INTRODUCTION .............................................................................................................................. 2
Parts list ...................................................................................................................................... 2
Unpacking................................................................................................................................... 2
INSTRUMENT DESCRIPTION ........................................................................................................ 3
The Amplier.............................................................................................................................. 3
Electrode Impedance............................................................................................................... 3
Filters............................................................................................................................................ 3
AC or DC Recording?................................................................................................................ 5
MAINTENANCE ................................................................................................................................ 5
Testing the Amplier ................................................................................................................ 5
SPECIFICATIONS.............................................................................................................................. 6
APPENDIX A DAM50 WIRING CONFIGURATIONS ................................................................... 7
A.300647 Prewired cable for metal electrodes................................................................. 7
a.Dierential conguration (Mode A-B) ......................................................................... 7
b.Single ended conguration (Mode A) ......................................................................... 7
B.5489 mini-banana adaptor for glass ................................................................................ 8
a.Dierential conguration (Mode A-B) ......................................................................... 8
b.Single ended conguration (Mode A) ......................................................................... 8
C.5447 2 mm adaptor for glass............................................................................................. 9
a.Dierential conguration (Mode A-B) ......................................................................... 9
b.Single ended conguration (Mode A) ......................................................................... 9
DECLARATION OF CONFORMITY ..............................................................................................10
WARRANTY .....................................................................................................................................11
Claims and Returns ................................................................................................................11
Repairs.......................................................................................................................................11
Copyright © 2018 by World Precision Instruments. All rights reserved. No part of this publication may
be reproduced or translated into any language, in any form, without prior written permission of World
Precision Instruments, Inc.

ii World Precision Instruments

DAM50
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The DAM50 is a dierential amplier.

2 World Precision Instruments
INTRODUCTION
Dierential amplication is of great importance in bioelectric
recording to reduce the ever present eect of noise
interference from power line induction. A well designed
dierential amplier will signicantly diminish power line
(mains) noise. It is most essential that the preparation be
connected with an electrode to a good electrical ground
as well as to the grounding wire of the DAM50 itself. This
should have the eect of greatly reducing electrostatically
induced potential. In addition to the preparation ground,
two dierential input connections must be made via
appropriate electrodes applied to the recording site so as to
optimally record a bioelectric potential dierence.
Parts list
Contents
DAM50 amplier
3518 shielded cable (bare termination) (available in a package of two as P/N 3517).
300647 shielded cable for metal electrodes
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 11 of this manual. Please contact WPI Customer
Service if any parts are missing at 941.371.1003 or [email protected].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper or
plastic surrounded with at least 100mm (four inches) of shock absorbing material. For
further details, please read the section entitled “Claims and Returns” on page 11 of
this manual.

DAM50
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INSTRUMENT DESCRIPTION
The Amplier
INPUT to the DAM50 amplier is made via a exible, 4-wire shielded cable terminated
in a miniature telephone-style plug. The DAM50 now has a prewired cable (300647)
made for metal electrodes (0.031” pin) and a reference electrode on a 2 mm pin. Refer
to the appendix for wiring congurations.
The designation of the four wires is indicated on the mating panel receptacle. The
INPUT SELECT switch allows the user to choose A, B (inverting) or dierential A-B.
It is recommended that, while the electrodes are being connected, amplier inputs
should be grounded with the INPUT SELECT switch to avoid input overload which may
result in excessive recovery time as described below. Further, inputs A and -B must
be connected to a ground return path. In A-B mode, if either input is not placed on
the preparation, it must be connected to ground. Note that in A or -B INPUT SELECT
modes, the input not selected is grounded internally. The recorder or oscilloscope is
connected via the cable provided to the OUTPUT connector on the right side of the
amplier case.
Amplication is controlled by the GAIN selector switch. Note that amplication is ten
times larger in the AC mode than in the DC mode.
Electrode Impedance
If electrodes of very high impedance are used, electrostatic induction will induce
objectionably large noise signals. It may then be necessary to enclose the electrode
and cable with an electrostatic shield (keep the cable length short) or alternatively to
shield the entire recording area (The DAM80, a companion instrument in the DAM
amplier series, is especially designed for high impedance electrode recording).
Filters
The LOW FILTER selector switch determines the lower cut o frequency when the user
is in the AC MODE. Note that at 0.1 Hz setting the amplier may take a long time to
recover if the user inadvertently disconnects an input wire or if the input is exposed
to an excessively large artifact. This is a typical property of AC ampliers set at very low
frequency lter settings. For fast recovery from signal overload the user is advised to
operate at the highest frequency setting of the low frequency lter consistent with the
application. Generally, slow signals such as ECG and EEG require the 0.1 Hz setting,
while nerve action potential recording allows the use of the 10 Hz LOW FILTER setting
without sacricing signal quality.

4 World Precision Instruments
Fig. 1—GAINS: This chart shows the standard 3dB frequency cuto at maximum
lter band pass.

DAM50
World Precision Instruments 5
AC or DC Recording?
It should be noted that DC recording with metal electrodes will invariably result in
excessively large galvanic potentials being applied to the amplier’s input terminals.
This can result in overdriving the amplier so that the POSITION control will not be
able to restore the operating baseline’s position. In this case, use Calomel or Ag-AgCl
electrodes, or use an external variable bucking potential; alternatively, resort to AC
recording.
MAINTENANCE
Testing the Amplier
Instrument POWER is provided by two alkaline 9 volt batteries. Battery operation
results in lower internal noise level. Batteries can be tested by activating the BAT/CAL
switch. If a short tone is heard, the batteries are functional. Conversely if the tone is
absent, the batteries must be replaced. An additional function that the BAT/CAL switch
performs is to provide a low frequency square wave test signal which is applied to the
amplier input. The amplitude of this signal is 100 microvolts when operating in either
of the two highest GAIN positions and 1 millivolt in the lowest GAIN position.
Mode Gain Cal signal Output
DC 10× 1 mV 10 mV
DC 100× 1 mV 100 mV
DC 1000× 100 µV 100 mV
AC 100× 1 mV 100 mV
AC 1000× 1 mV 1000 mV
AC 10,000× 100 µV 1000 mV

6 World Precision Instruments
SPECIFICATIONS
This unit conforms to the following specications:
Input Resistance ...............................................>1012 Ohms, common mode and dierential
Input Leakage Current .................................................................................................50 pA, max.
Amplication ....................................................................................... 100×, 1000×, 10,000× (AC)
........................................................................................................................10×, 100×, 1000× (DC)
Common Mode Rejection Ratio (CMRR) .......................................... 100 dB typ. @ 50/60 Hz,
..............................................................................................................................80 dB typ. @ 1 KHz
Equivalent Noise Signal Input (ENSI) ..............................................................................................
AC amplier (typ.)............................................................... 0.4 µV rms (2.0 µV p/p), 0.1-100 Hz
................................................................................................2.0 µV rms (10 µVp/p), 1 Hz-10 kHz
DC Amplier .............................................................................6.0 µV rms (30 µV p/p), 3-10 kHz
Bandwidth Filter Settings ..................................................................................................................
AC ................................................................................................low frequency 0.1, 1, 10, 300 Hz
AC and DC .......................................................................... high frequency 100 Hz, 1, 3, 10 KHz
Position .................................................................................................................................... 250mV
Output Voltage Swing............................................................................................................... ± 8 V
Output Resistance...........................................................................................................470 Ohms
Battery Test.................................................................................................1 second audible tone
Calibrator Signal.........................................10 Hz square wave, amplitude 100 µV (at 1000×
.................................................................................. and 10,000×), 1 mV (at 100×) and 10× DC).
Power.......................................................................................... 2 9V alkaline batteries, supplied
Input Cables........ 1 shielded cable with metal electrode connectors are provided and 1
shielded cable with bare wires.
APPENDIX A DAM50 WIRING CONFIGURATIONS
PART NUMBER DESCRIPTION
M3301EH Replacement Electrode Holder
M3301R Manual Micromanipulator
MD4R Dual Tool-Holder Micromanipulator
TM31A10 Tungsten Metal Electrodes Prole A, 76 mm, 1 micron
RC1T Reference Cells
M10 M10 Magnetic Stand
5052 Steel Base Plate, 10 lbs
3294 Ground Wire for DAM80 Probe
EP2 Ag/AgCl Electrode
2505 Electrode Handle 6.3 mm
5444 Electrode Handle 4.8 mm
13620 Low-noise cable for Microelectrode Holder
5489 Optional Probe for DAM50
MEH7W-10 Microelectrode Holder

DAM50
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M3301EH
M3301R
TM31A10
DAM50
RC1T 300647
M3301EH
TM31A10
DAM50
MD4R
5052
M10
RC1T
300647
b. Single ended conguration (Mode A)
A. 300647 Prewired cable for metal electrodes
a. Dierential conguration (Mode A-B)

8 World Precision Instruments
B. 5489 mini-banana adaptor for glass
a. Dierential conguration (Mode A-B)
b. Single ended conguration (Mode A)
DAM50
DAM50
EP2
EP2
13620
13388
2505
2505
2505
5444
5444
5489
5489
3294
3294
MEH7W10
MEH7W10
13620
13620

DAM50
World Precision Instruments 9
C. 5447 2 mm adaptor for glass
a. Dierential conguration (Mode A-B)
DAM50
13620
2505
5444
MEH7W10
MEH7W1013620
RC1T
5447
b. Single ended conguration (Mode A)
DAM50
5447
5444
2505
RC1T
13620

10 World Precision Instruments
DECLARATION OF CONFORMITY

DAM50
World Precision Instruments 11
* Electrodes, batteries and other consumable parts are warranted for 30 days only from the date on which
the customer receives these items.
WARRANTY
WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment, including
its components and parts, shall be free from defects in material and workmanship for a period of
one year* from the date of receipt. WPI’s obligation under this warranty shall be limited to repair or
replacement, at WPI’s option, of the equipment or defective components or parts upon receipt thereof
f.o.b. WPI, Sarasota, Florida U.S.A. Return of a repaired instrument shall be f.o.b. Sarasota.
The above warranty is contingent upon normal usage and does not cover products which have been
modied without WPI’s approval or which have been subjected to unusual physical or electrical stress
or on which the original identication marks have been removed or altered. The above warranty will not
apply if adjustment, repair or parts replacement is required because of accident, neglect, misuse, failure
of electric power, air conditioning, humidity control, or causes other than normal and ordinary usage.
To the extent that any of its equipment is furnished by a manufacturer other than WPI, the foregoing
warranty shall be applicable only to the extent of the warranty furnished by such other manufacturer.
This warranty will not apply to appearance terms, such as knobs, handles, dials or the like.
WPI makes no warranty of any kind, express or implied or statutory, including without limitation any
warranties of merchantability and/or tness for a particular purpose. WPI shall not be liable for any
damages, whether direct, indirect, special or consequential arising from a failure of this product to
operate in the manner desired by the user. WPI shall not be liable for any damage to data or property
that may be caused directly or indirectly by use of this product.
Claims and Returns
Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted on
the delivery receipt before signing. Concealed loss or damage should be reported at once to the carrier
and an inspection requested. All claims for shortage or damage must be made within ten (10) days
after receipt of shipment. Claims for lost shipments must be made within thirty (30) days of receipt of
invoice or other notication of shipment. Please save damaged or pilfered cartons until claim is settled.
In some instances, photographic documentation may be required. Some items are time-sensitive; WPI
assumes no extended warranty or any liability for use beyond the date specied on the container
Do not return any goods to us without obtaining prior approval and instructions from our Returns
Department. Goods returned (unauthorized) by collect freight may be refused. Goods accepted for
restocking will be exchanged or credited to your WPI account. Goods returned which were ordered
by customers in error are subject to a 25% restocking charge. Equipment which was built as a special
order cannot be returned.
Repairs
Contact our Customer Service Department for assistance in the repair of apparatus. Do not return
goods until instructions have been received. Returned items must be securely packed to prevent
further damage in transit. The Customer is responsible for paying shipping expenses, including
adequate insurance on all items returned for repairs. Identication of the item(s) by model number,
name, as well as complete description of the diculties experienced should be written on the repair
purchase order and on a tag attached to the item.

12 World Precision Instruments
USA
International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258
Tel: [phone] • Fax: [phone] • E-mail: [email protected]
UK
1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ
Tel: [phone] • Fax: [phone] • E-mail: [email protected]
Germany
Saarstraße 23, D-61169 Friedberg (Hesson), Germany
Tel: [phone] • Fax: [phone] • E-mail: [email protected]
China & Hong Kong
WPI Shanghai Trading Co., Ltd.
Rm 25e, No8 Dongfang Rd., Pudong District, Shanghai, 200120 PR China
Tel: [phone] • E-mail:[email protected]
Brazil
Av. Conselheiro Nébias, 756 sala 2611, Santos-CEP: 11045-002, São Paulo Brazil Tel: (013) 406-
29703 • E-mail: [email protected]
Internet
www.wpiinc.com • www.wpi-europe.com • www.wpiinc.cn • www.wpibrasil.com.br