WPI SI-MT User manual

INSTRUCTION MANUAL
Serial No._____________________
112014
World Precision Instruments
www.wpiinc.com
SI-MT/SI-MKB/SI-HTB
Signal Conditioning Amplifier System for Muscle Testers

SI-H Muscle Testers
II WORLD PRECISION INSTRUMENTS
Other Popular WPI Products
The new SI-BF-100 is an LED-based
fluorometer for life science applications. It is
ideally suited for ratiometric calcium detection
(FURA-2) and ATPase detection (via NADH
fluorescence). With up to seven LED modules
(wavelengths), the SI-BF-100 covers many
fluorometric applications in neuroscience and
cell biology.
Recent advancements in optics and LED
technology simplify ratiometric calcium
imaging, making this equipment more
affordable. A breakthrough in WPI patented
technology allows the SI-BF-100 to use
wavelengths below 380nm and produce
more light in those spectra. This technology
significantly cuts the cost of photometric
calcium imaging without sacrificing resolution
or quality.
• LED light sources require less power,
give off less heat and are more
compact and affordable
• Sampling rates up to 1kHz (1,000
ratios/second maximum). At lower
speeds, signal averaging is used for
noise reduction.
• Two auto ranging photomultiplier
inputs allow you to monitor multiple
wavelengths from a single emission
output that can be comprised of
Biofluorometer
Now more reliable,
simplified and affordable
any wavelength of light for which an LED
module is available
• Using a separate reference channel,
ultra-stable, continuous ratio calculations
automatically compensate for LED
intensity drift. This ensures less noise and
produces more accurate measurements.
• Application-specific probes are available
for existing tissue baths and cuvette
systems.
• Ratio noise is <0.05 peak to peak, drift is
less than 0.1 unit/hour
• The warm up time of less than one
minute is a dramatic improvement over
the common 20–60 minutes required by
xenon or mercury light sources
• Replace the emission filter easily or
change the LED modules to transform
the SI-BF-100 into a general purpose
fluorometer for many other applications
Tip Profile:
1:1
McPherson-Vannas Scissors
Length: 8 cm (3 in.)
Blades: straight 5 mm
Tips: 0.1mm
Length: 8 cm (3 in.)
Blades: curved 5 mm
Tips: 0.1mm
Tip Profile: Tip Profile:
2.3 cm
Iris Scissors, stainless steel
Length: 11.5 cm (4.5 in.)
Blades: straight, Tungsten Carbide
Standard: 500216
German: 500216-G
Length: 11.5 cm (4.5 in.)
Blades: curved, Tungsten Carbide
Standard: 500217
German: 500217-G

SI-MT/SI-MKB/SI-HTB
WORLD PRECISION INSTRUMENTS iii
Copyright © 2014 by World Precision Instruments, Inc. All rights reserved. No part of this publication may be
reproduced or translated into any language, in any form, without prior written permission of World Precision
Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ............................................................................................................................... 1
INTRODUCTION ........................................................................................................................................... 2
Features...................................................................................................................................................... 3
Cautions and Warnings ........................................................................................................................ 3
Parts List ..................................................................................................................................................... 3
Unpacking ................................................................................................................................................. 3
INSTRUMENT DESCRIPTION.................................................................................................................... 4
Signal Conditioning Amplifier (SI-MT/SI-MKB/SI-HTB) .............................................................. 4
Front Panel .......................................................................................................................................... 4
Back Panel........................................................................................................................................... 5
SI-BAM21-LCB .......................................................................................................................................... 6
Features ................................................................................................................................................ 6
How the Amplifier Works............................................................................................................... 6
Notes and Warnings ........................................................................................................................ 6
Front Panel .......................................................................................................................................... 7
SI-PF100 ..................................................................................................................................................... 8
SI-AOSUB ................................................................................................................................................... 9
Front Panel .......................................................................................................................................... 9
SI-SARCAM ..............................................................................................................................................10
Front Panel ........................................................................................................................................12
SI-MOTDB ................................................................................................................................................13
Features ..............................................................................................................................................14
Front Panel ........................................................................................................................................15
SI-TCM2B .................................................................................................................................................16
Features....................................................................................................................................................16
Front Panel ........................................................................................................................................16
SI-COLUB..................................................................................................................................................17
Connecting the Signal Conditioning Amplifier System............................................................22
OPERATING INSTRUCTIONS...................................................................................................................25
Turning the System On .......................................................................................................................25
Using the SI-BAM21-LCB ....................................................................................................................25
Calibrating the SI-BAM21-LCB ...................................................................................................25
Making Measurements .......................................................................................................................28
Setting System Gain Factor ..........................................................................................................28
Using the Programmable Filter ........................................................................................................28
Using the Anti-Oscillation Unit .........................................................................................................29
Adjusting the Anti-Oscillation Filter...........................................................................................29
Using the Sarcomere Length Unit ...................................................................................................31
Setup....................................................................................................................................................31
Choosing a Display Mode............................................................................................................31
Setup Menu .......................................................................................................................................31
1:1

SI-H Muscle Testers
IV WORLD PRECISION INSTRUMENTS
Turning On/Off the Laser .............................................................................................................32
Adjusting the CCD Offset..............................................................................................................32
Setting the Tissue Distance..........................................................................................................32
Adjusting the CCD Sensitivity......................................................................................................33
Setting the Camera (CCD) Mode ................................................................................................33
Aligning the Laser and the Camera..........................................................................................33
Calibrating the Camera .................................................................................................................35
Display Laser Diffraction Pattern on an Oscilloscope ........................................................36
Understanding the Display ..........................................................................................................36
Setup....................................................................................................................................................37
Choosing a Display Mode............................................................................................................37
Setup Menu .......................................................................................................................................37
Adjusting the Setpoint ...................................................................................................................38
Setting Alarms ..................................................................................................................................38
Changing the Backlight Level for the Display.......................................................................38
Enabling/Disabling the Alarms...................................................................................................39
Using the USB Port Output ..........................................................................................................39
MAINTENANCE............................................................................................................................................40
ACCESSORIES...............................................................................................................................................40
TROUBLESHOOTING.................................................................................................................................41
SPECIFICATIONS..........................................................................................................................................42
INDEX .............................................................................................................................................................43
WARRANTY ..................................................................................................................................................44
Claims and Returns ..............................................................................................................................44

SI-MT/SI-MKB/SI-HTB
WORLD PRECISION INSTRUMENTS 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1 (Left) SI-MT is a convenient platform for all standard mechanical experiments on intact
muscle preparations.
Fig. 2 (Right) The SI-MKB platform can be used for mechanical and optical studies of intact or
skinned muscle fibers using optional electronic components that are discussed in this manual.

SI-H Muscle Testers
2WORLD PRECISION INSTRUMENTS
Fig. 3 The Signal Conditioning Amplifier System is a flexible chassis that is specifically
configured for an SI-MT, SI-HTB or SI-MKB system (Muscle Testers).
INTRODUCTION
The Signal Conditioning Amplifier System provides a flexible electronic platform intended
to process the transduction of mechanical signals, the filtering of transducer outputs and
the control of motor positions.
The system consists of an 8-slot, rack-mountable frame that includes an ultra quiet,
shielded power supply. Outputs are routed internally to the inputs of other modules. If you
prefer, the module outputs may be routed to external outputs on the front panels. The
system has a small footprint and may be stacked to provide as many optional modules as
you need.
When the system is ordered with an SI-MT, SI-HTB or SI-MKB (Muscle Tester) system,
the Signal Conditioning Amplifier System (chassis) is configured with an SI-BAM21-LCB.
Optional modules include an SI-TCM2B Temperature Control Module, an SI-MOTDB
Linear Motor Controller, an SI-AOSUB Anti Oscillation Unit, the SI-SARCAM Sarcomere
Spacing Module and the SI-COLU Constant Load Unit. The Temperature Control Module,
Linear Motor Controller and Sarcomere Spacing Module require two slots each on the
chassis backplane.
NOTE: The system is flexible and configurable. A variety of modules are available for the
Signal Conditioning Amplifier System, and you can mix and match the modules to suit
your requirements. For this manual, we will only discuss the modules used with an SI-MT,
SI-HTB or SI-MKB system.

SI-MT/SI-MKB/SI-HTB
WORLD PRECISION INSTRUMENTS 3
Features
This Signal Conditioning Amplifier System offers eight expansion slots, configured at the
factory to meet your requirements.
NOTE: The system is configured at the factory. If you need to add additional modules,
contact Technical Support at 941.371.1003 or [e-mail].
Cautions and Warnings
WARNING: TURN OFF THE SIGNAL CONDITIONING AMPLIFIER SYSTEM AND
UNPLUG IT FROM THE POWER OUTLET BEFORE REMOVING OR INSTALLING
ANY MODULE IN THE UNIT.
Parts List
After unpacking, verify that there is no visible damage to the instrument. Verify that all
items are included:
(1) Signal Conditioning Amplifier System with an SI-BAM21-LCB
(1) Power cord
(1) Instruction Manual
OPTIONAL COMPONENTS:
SI-TCM2B Temperature Control module
SI-MOTDB Linear Motor Control module
SI-PF100 Programmable Filter module
SI-AOSUB Anti-Oscillation module
SI-SARCAM Sarcomere Spacing module (For use with the Muscle Tester only.)
SI-COLU Constant Load Unit
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and check
for possible damage. Missing cartons or obvious damage to cartons should be noted on
the delivery receipt before signing. Concealed damage should be reported at once to the
carrier and an inspection requested. Please read the section entitled “Claims and Returns”
on page 44 of this manual. Please contact WPI Customer Service if any parts are
missing at 941.371.1003 or [e-mail].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA #
required) and instructions from WPI’s Returns Department. Goods returned (unauthorized)
by collect freight may be refused. If a return shipment is necessary, use the original
container, if possible. If the original container is not available, use a suitable substitute
that is rigid and of adequate size. Wrap the instrument in paper or plastic surrounded with
at least 100mm (four inches) of shock absorbing material. For further details, please read
the section entitled “Claims and Returns” on page 44 of this manual.

SI-H Muscle Testers
4WORLD PRECISION INSTRUMENTS
INSTRUMENT DESCRIPTION
Signal Conditioning Amplifier (SI-MT/SI-MKB/SI-HTB)
Front Panel
Power
Switch
Sarcomere
Spacing
Module
Anti
Oscillation
Unit
Optical
Transducer
Amplifier
Linear
Motor
Controller
Temperature
Control
Module
Fig. 4 The front panel of a Signal Conditioning Amplifier System configured for a Muscle Tester
shows the SI-BAM21-LCB, the SI-AOSUB, The SI-SARCAM, the SI-MOTDB and the SI-TCMB2.
Optical Transducer Amplifier–The SI-BAM21-LCB powers the force transducer and
converts the output of the transducer to an amplified analog voltage that is proportional
to the force applied to the transducer. The output signal can be multiplied by a factor of 1,
2, 5 or 10 to provide better resolution for a minimal change in applied force.
Programmable Filter (SI-PF100)–(Not shown) This low pass filter may be used in place of
an Anti-Oscillation unit to program the cutoff equivalence. You may select a Bessel or a
Butterworth filter and set the cutoff frequency.
Anti-Oscillation Unit (SI-AOSUB)–Each force transducer has a resonance frequency at
which it vibrates. The SI-AOSUB, when properly tuned to that resonance frequency,
removes the resonance noise from the output signal of SI-BAM21-LCB transducer
amplifier. An SI-AOSUB is necessary when a linear motor is used.
Sarcomere Spacing Module–Sarcomere spacing measurement requires a system with
an optical cuvette (cuvette with a window). A laser passes through the muscle, and the
diffracted light is captured with a CCD camera. By monitoring the diffraction pattern, the
length of the sarcomeres can be calculated.
Linear Motor Control Module–When a linear motor is required, this module powers
the motor and provides an output indicating the actual motor position. It connects to
an analog to digital converter output of the data acquisition system (like LabTrax 8/16)
to control the waveform and timing for the motor control. The output connects with an
analog input of the data acquisition system to monitor the sensor feedback from the motor.

SI-MT/SI-MKB/SI-HTB
WORLD PRECISION INSTRUMENTS 5
Temperature Control Module–When temperature control is required, the SI-TCM2
is used. It can control two cuvettes simultaneously, using digital control to maintain
a constant temperature. It has both high and low alarm warnings which can be user
defined.
Constant Load Module–(Not pictured in the unit here.) It is often important to maintain a
constant sarcomere length rather than keep the total length of the preparation constant
during an isometric contraction. For example, if the muscle is fixed with its tendons, the
muscle contraction elongates these tendons. Even though the distance between the ends
of the muscle is kept constant, the muscle contraction effects an internal shortening of
the sarcomeres of the muscle. In order to get a true isometric muscle contraction, the
sarcomere length must be kept constant during the contraction. For this purpose the
output signal of the camera (SI-SARCAM) can be fed into the feedback controlling the
linear motor position using the SI-COLU. The feedback system controls the linear motor
so that the sarcomere length is held constant.
Expansion Slots–Empty slots (not shown) on the back plane are filled with expansion
slots which can be replaced at a later time when other modules are added to the system.
Power Switch–This system has two power switches, one on the back panel and one on
the front. Both switches must be on to power the system.
Back Panel
Master Power SwitchFuse Housin
g
Power Connector
Fig. 5 The back panel of the Signal Conditioning Amplifier System has a master power switch
that is usually left on.
Power Connector–Insert the power cord into the power connector, and plug the cord into
a standard wall AC outlet.
Fuse Housing–This housing contains the fuse for the chassis system.
Master Power Switch–The signal conditioning chassis distributes sub-regulated DC power
(12V) to the individual modules through a backplane of the chassis. For convenience, the
unit has two power switches, and both must be on to power the system. All the modules
power on/off simultaneously. When your system is set up, just leave this power switch in
the on (I) position

SI-H Muscle Testers
6WORLD PRECISION INSTRUMENTS
NOTE: The 16 plugs marked with A or B are for future development. They are not used at
this time.
SI-BAM21-LCB
The SI-BAM21-LCB KG Optical Force Transducer Amplifier is used in conjunction with the
SI-H tissue bath and muscle physiology systems. The SI-BAM21-LCB powers the force
transducer and converts the output of the transducer to an amplified analog voltage
that is proportional to the force applied to the force transducer. The output signal can be
multiplied by a factor of 1, 2, 5 or 10 to provide better resolution for a minimal change in
applied force.
NOTE: An optional factory setting increases the multiplier by a factor of 10, allowing the
signal to be multiplied by 10, 20, 50 and 100.
NOTE: The SI-BAM21-LC is the standalone version of this optical force transducer
amplifier.
Features
The SI-BAM21-LCB amplifier works with KG optical force transducers to:
• Generate an analog output (-10VDC to +10VDC) that is proportional to the force
applied to the tissue sample.
• Supply a DC voltage that powers the KG force transducer to which it is
connected.
How the Amplifier Works
In a typical setup, a muscle is held by a force transducer. The force transducer is
connected to the SI-BAM21-LCB. As the muscle contracts or releases, the force transducer
converts the force into an electrical current signal which is proportional to the force
applied to the force transducer. The SI-BAM21-LCB converts the current signal into a
voltage signal that can be displayed on the screen of the recording device.
Before initiating an experiment, the SI-BAM21-LCB must first be zeroed. This sets the
baseline for measurements to follow.
The output signal is buffered and multiplied by 1, 2, 5 or 10, depending on the Gain
switch setting on the front panel of the amplifier module. The X10 setting is useful when
output signals are extremely small. Finally, the force proportional signal is sent through
the output amplifier circuit.
The analog output has a range of –10V to +10V that drives a data acquisition system,
multimeter or oscilloscope.
Notes and Warnings
NOTE: The SI-BAM21-LCB is only designed for use with KG optical force transducers.
Use with any other type of transducer may cause damage to either the transducer or the
amplifier or both.