WPI PZMIII User manual

PZMIII/PZMTIII
Precision Zoom Stereo Microscope
World Precision Instruments
INSTRUCTION MANUAL
Serial No._____________________
101212
www.wpiinc.com

PZMIII/PZMTIII
World Precision Instruments iii
Copyright © 2012 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ..................................................................................................................... 1
INTRODUCTION.................................................................................................................................. 2
Parts List ........................................................................................................................................... 2
Unpacking ....................................................................................................................................... 2
INSTRUMENT DESCRIPTION.......................................................................................................... 3
Setup.................................................................................................................................................. 4
Adusting a Microscope................................................................................................................ 4
System Diagram ............................................................................................................................ 5
OPERATING INSTRUCTIONS........................................................................................................... 6
MAINTENANCE.................................................................................................................................... 7
ACCESSORIES....................................................................................................................................... 8
SPECIFICATIONS.................................................................................................................................. 9
PZMIII Eyepieces and Objectives ............................................................................................ 9
APPENDIX: BOOM STAND ASSEMBLY......................................................................................10
Focus Mount .................................................................................................................................10
WARRANTY ........................................................................................................................................11
Claims and Returns ....................................................................................................................11
Repairs ............................................................................................................................................11

iv World Precision Instruments

PZMIII/PZMTIII
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can
cause damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can
cause personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The PZMIII binocular microscope is shown on its standard post stand.

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INTRODUCTION
WPI’s PZMIII stereomicroscope is an ideal tool for tissue dissection, cell injection,
specimen manipulation, electrode inspection and many other applications
requiring a magnified stereo view and ample working distance. It offers quality and
performance at an affordable price. Advanced optics provide image sharpness found
only among the best of this class.
Zooming is smooth and effortless, controlled by a spring-loaded knob. The PZMIII’s
compact size and light weight make it more stable and easily manipulated on a
boom stand. The binocular PZMIII can be eaily upgraded to a trinocular microscope
at any time by attaching the optional Photo/Video Module.
With an extensive array of optional accessories, PZMIII is suitable for almost any
bio-research application requiring a stereo microscope.
Parts List
After unpacking, verify that there is no visible damage to the sensor. Verify that all
items are included:
(1) PZMIII or PZMTIII Stereo microscope head
(1) Post stand
(1) Light ring adapter
(2) 10X eyepieces
(2) Paired Eye Guards
(1) Instruction Manual
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 11 of this manual. Please contact WPI Customer
Service if any parts are missing at 941.371.1003 or [e-mail].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper
or plastic surrounded with at least 100mm (four inches) of shock absorbing material.
For further details, please read the section entitled “Claims and Returns” on page
11 of this manual.

PZMIII/PZMTIII
World Precision Instruments 3
INSTRUMENT DESCRIPTION
Diopter Adjustment Ring
Magnification Indicating Ring
Post
Base
Focusing Knob
Magnification Knob
Stage Plate
Eyepiece
Body Rotation Clamp
Locking Screw Securing Knob
Vertical Stop Knob
PZMTIII
CCD Color Video
Camera (optional)
C-Mount CCD Camera
Coupler (optional)
Video/Photo Port
Light Path
Selector Bar*
Fig. 2—This image identifies the parts of the binocular PZMIII. The trinocular version
(PZMTIII) has the video/photo port and the light path selector bar, and it can accept the
camera with a C-Mount coupler.
*NOTE: “True trinocular” microscopes do not have the Light Path Selector Bar.
Instead of blocking the right eye path, this port transfers 50% of the light intensity to
the camera port.

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Setup
1. Remove the microscope from the case and check that the following parts are all
present and undamaged:
• Microscope and Base
• WFH 10x Wide-Field Eyepieces (two)
• Paired Eyeguards (two)
• Stage Plate
• Instruction Manual
2. Unscrew the protective cap on the bottom of the microscope head.
3. Refer to the “Instrument Description” on page 3 and follow the set-up
instructions below.
4. Loosen the securing knob to place the microscope at approximately the
working distance.
5. The exact working distance is found by adjusting the focusing knob.
6. Install the WFH 10x eyepiece pair, included. Then, attach the paired eyeguards
to the eyepieces.
7. When the optional stands are used instead of the standard base, see the
“System Diagram” on page 5 for mounting instructions.
8. The Precision Zoom Stereo Microscope is now ready for normal operation.
Adusting a Microscope
These instructions describe how to properly focus a binocular microscope.
1. Turn on the lamp and adjust its intensity.
2. Set theoth eye piece diopters to zero.
3. Adjust the interpupilary distance so that the two circles of light merge into one.
4. Place a sample on the stage.
5. Select the 1.0 dial setting and look through the right eyepiece only.
6. Use the focus control knobs to adjust the focus of the sample.
7. When the sample is clearly visible, use only your left eye. Do NOT adjust the
focus knobs. Instead, adjust the diopter on the left eyepiece until the sample
comes clearly into view. The image should be clear with both eyes open.
8. Choose the desired zoom range. You may need to adjust the fine focus a little.
TIP: For information on microscope basics, understanding objectives, choosing a
microscope camera, a camera comparison chart and a camera/microscope
configuration chart, see www.wpiinc.com/microscope.

PZMIII/PZMTIII
World Precision Instruments 5
System Diagram
Post Stand
503102
Fan Stand
501353
Articulated Arm
with Table Clamp: 502007
Optional Wall Mount: 502163
Boom/Clamp Stand
502006
Extension Rod
504123
Universal
Focus
Mount
502009
Ring Light Adapter
13338*
*Included with the body
Objectives:
0.3X–501373
0.5X–501375
1.5X–501377
2.0X–501378
Eyepieces:
10X–501369*
15X–501370
20X–501371
25X–501372
C-mount to Eyepiece Adapter:
1/3” or 1/2” Video–503097
1/4” Video–503098
2X for DSLR–503099
CCD Coupler:
0.5X–501381
1.0X–503300 (special order only)
1/2” Color CCD Camera
USBCAM50 Color CCD Video Camera
COLCAM-HD
Color CCD Video Camera
COLCAM
Ring Light
504134 Fiber Optic Light
NOVA
BoomStand
502004
Ball-Bearing Stand
502005
Binocular Body
501352
Trinocular Body
501379
Fig. 3—This diagram shows many of the options available for the PZMIII.

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OPERATING INSTRUCTIONS
Refer to Fig. 2 on page 3 and Fig. 3 on page 5 along with the following
instructions on normal operation.
1. Working Distance Adjustment–Loosen the Securing knob and the Vertical Stop
knob which secures the microscope to the post. Move the microscope to the
desired height (working distance) according to lens (auxiliary objective lens)
attached (see “PZMIII Eyepieces and Objectives” on page 9 for working
distance). Then, re-tighten the knobs.
2. Interpupilary Distance Adjustment–Looking through the eyepieces with both
eyes, adjust the interpupillary distance by sliding the two eyepiece tubes with
both hands until a single image is observed.
3. Focus Adjustment–Place a sample under the microscope, turn the Magnification
knob to its greatest setting (4.5). Focus the image by turning the Focusing knob.
Once the image is in clear focus at its highest magnification you may zoom out
to lower magnification by adjusting the Magnification knob while remaining in
focus.
4. Diopter Adjustment–If the image does go out of focus when zooming out, zoom
to the lowest setting and focus each eyepiece individually by adjusting the
Diopter Adjustment Ring.
5. Video/Photo Applications (for the Trinocular PZMTIII only)–Slide the Light Path
Selector bar* to the Photo In position in order to direct the light to the Video/
Photo port. Remove the black protective cap on top of the Video/Photo port.
Slip and secure the body of C-mount CCD camera Coupler over the Video/Photo
port, with its focus ring facing back. Attach a C-mount video or digital camera
(when using cameras with an integrated lens filter thread of 28, 37 or 43 mm,
Digital Camera Adapter Kit #501384 is required). Refer to your camera’s manual
for instructions on connecting the camera to the monitor. Focus the image on
the screen by adjusting the Fine Focus Ring located at the back of the C-mount
CCD camera coupler.
*NOTE: The newer trinocular body is a true trinocular. It may not have the Light
Path Selector switch. This microsocpe port transfers 50% of the light intensity to the
camera port without blocking the right eye piece path.