WPI PZMIV User manual

PZMIV/PZMTIV
Precision Zoom Stereo Microscope
World Precision Instruments
INSTRUCTION MANUAL
Serial No._____________________
040612
www.wpiinc.com

PZMIV/PZMTIV
World Precision Instruments iii
Copyright © 2012 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ..................................................................................................................... 1
INTRODUCTION.................................................................................................................................. 2
Parts List ........................................................................................................................................... 2
Unpacking ....................................................................................................................................... 2
INSTRUMENT DESCRIPTION.......................................................................................................... 3
Setup.................................................................................................................................................. 4
Adusting a Microscope................................................................................................................ 4
System Diagram ...................................................................................................................... 5
OPERATING INSTRUCTIONS........................................................................................................... 6
MAINTENANCE.................................................................................................................................... 7
ACCESSORIES....................................................................................................................................... 8
SPECIFICATIONS.................................................................................................................................. 9
PZMIV & PZMTIV Eyepieces and Objectives .........................................................9
APPENDIX: BOOM STAND ASSEMBLY......................................................................................10
Focus Mount .................................................................................................................................10
WARRANTY ........................................................................................................................................11
Claims and Returns ....................................................................................................................11
Repairs ............................................................................................................................................11

iv World Precision Instruments

PZMIV/PZMTIV
World Precision Instruments 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can
cause damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can
cause personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1—The PZMIV binocular microscope is shown on its standard track stand.
Max. Working Range: 25 cm

2 World Precision Instruments
INTRODUCTION
WPI’s PZMIV stereo microscope is an ideal tool for tissue dissection, cell injection,
specimen manipulation, electrode inspection and many other applications
requiring a magnified stereo view and ample working distance. It offers quality and
performance at an affordable price. Advanced optics provide image sharpness found
only among the best of this class.
PZMIV uses a modular, parallel-beam path design and a high quality optical system.
The advanced optical design with plan achromatic objectives provides sharp and
distortion-free image throughout the entire zoom range. The ergonomic design
makes it easy to use—zooming is smooth and effortless, controlled by a large spring-
loaded knob.
The PZMIV is available in a binocular or trincocular version. In addition, an
extensive list of optional accessories is available that makes the PZMIV suitable for
integrated optical and biological research. A variety of PZMIV microscope kits are
also sold.
The microscope comes with a track stand, standard 10x eyepieces (wide-field,
distortion-free and high eye point), and a 1x planachromatic distortion-free
objective.
Parts List
After unpacking, verify that there is no visible damage to the sensor. Verify that all
items are included:
(1) PZMIV Stereo microscope head
(1) Track stand
(1) Light ring adapter
(2) 10X eyepieces (wide-field, distortion-free and high eye point)
(2) Paired Eye Guards
(1) 1x planachromatic distortion-free objective
(1) Instruction Manual
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and
check for possible damage. Missing cartons or obvious damage to cartons should be
noted on the delivery receipt before signing. Concealed damage should be reported
at once to the carrier and an inspection requested. Please read the section entitled
“Claims and Returns” on page 11 of this manual. Please contact WPI Customer
Service if any parts are missing at 941.371.1003 or [e-mail].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA
# required) and instructions from WPI’s Returns Department. Goods returned
(unauthorized) by collect freight may be refused. If a return shipment is necessary,
use the original container, if possible. If the original container is not available, use a

PZMIV/PZMTIV
World Precision Instruments 3
suitable substitute that is rigid and of adequate size. Wrap the instrument in paper
or plastic surrounded with at least 100mm (four inches) of shock absorbing material.
For further details, please read the section entitled “Claims and Returns” on page 11 of
this manual.
INSTRUMENT DESCRIPTION
CCD Color Video
Camera (optional)
C-Mount CCD Camera
Coupler (optional)
Diopter Adjustment Ring
Magnification Indicating Ring*
Track
Base
Focusing Knob
Magnification Knob
Body Rotation Clamp
Locking Screw
Stage Plate
Video/Photo Port
Light Path Selector Bar** Eyepiece
*Above the right-hand knob
(hidden in this photo) is a detent
control to provide discreet zoom
stops or continuous zooming.
**When out, light to the right
eyepiece is switched to the
photo port
PZMTIV Only
Fig. 2—This image identifies the parts of the trinocular PZMTIV. The binocular version
(PZMIV) will not have the camera, video/photo port or the light path selector bar.

4 World Precision Instruments
Setup
1. Remove the microscope from the case and that the parts are all present and
undamaged. See “Parts List” on page 2.
2. Assemble the track stand.
3. Mount the microscope head into the focus mount.
4. Remove the protective cap on the bottom of the microscope head.
5. Refer to the parts diagram (Fig. 2) and follow the set-up instructions.
6. Install the WFH 10x eyepiece pair (included). The paired eyeguards can now be
attached to the eyepieces.
7. The exact working distance is found by adjusting the focusing knob.
8. When the optional stands are used instead of the standard base, see “System
Diagram” on page 5 for mounting instructions.
Adusting a Microscope
These instructions describe how to properly focus a binocular microscope.
1. Turn on the lamp and adjust its intensity.
2. Adjust the interpupilary distance so that the two circles of light merge into one.
3. Place a sample on the stage.
4. Select the 10X objective and look through the right eyepiece only.
5. Use the coarse and fine focus control knobs to adjust the focus of the sample.
6. When the sample is clearly visible, use only your left eye. Do NOT adjust the
focus knobs. Instead, adjust the diopter on the left eyepiece until the sample
comes clearly into view. The image should be clear with both eyes open.
7. Choose the desired objective. You may need to adjust the fine focus a little.
TIP: For information on microscope basics, understanding objectives, choosing a
microscope camera, a camera comparison chart and a camera/microscope
configuration chart, see www.wpiinc.com/microscope.

PZMIV/PZMTIV
World Precision Instruments 5
System Diagram
Track Stand
502002
Fan Stand
501353
Articulated Arm
with Table Clamp: 502007
Optional Wall Mount: 502163
Boom/Clamp Stand
502006
Extension Rod
504123
Universal
Focus
Mount
502009
Ring Light Adapter
502015*
*Included with the body
Objectives:
0.32X–502016
0.5X–502017
0.63X–502018
1.0X–502019*
Eyepieces:
10X–502010*
16X–502011
20X–502012
25X–502013
With Reticle:
10X–500264
20X–500266
C-mount to Eyepiece Adapter:
1/3” or 1/2” Video–503097
1/4” Video–503098
2X for DSLR–503099
CCD Coupler:
0.35X–500261
0.5X–500262
1X–500028
1/2” Color CCD Camera
USBCAM50 Color CCD Video Camera
COLCAM-HD
Color CCD Video Camera
COLCAM
Ring Light
504134 Fiber Optic Light
NOVA
BoomStand
502004
Ball-Bearing Stand
502005
Fig. 3—This diagram shows many of the options available for the PZMIV.

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OPERATING INSTRUCTIONS
Refer to Fig. 2 on page 3 and Fig. 3 on page 5 along with the following
instructions on normal operation.
1. Set up the microscope on the track stand. Mount the microscope head into the
focus mount. Use focusing knob to bring microscope to focus.
2. Looking through the eyepieces with both eyes, adjust the interpupillary distance
by sliding the two eyepiece tubes with both hands until a single image is
observed.
3. Place a sample under the microscope, turn magnification knob to its greatest
setting (5.0). Focus the image by turning the focusing knob. Once the image
is in clear focus at its highest magnification you may zoom out to lower
magnification by adjusting magnification knob while remaining in focus.
4. If image goes out of focus when zooming out, zoom to the lowest setting and
focus each eyepiece individually by adjusting the diopter adjustment ring.
5. For video or photo applications on the PZMTIV:
• Slide the light path selector bar to the “PHOTO IN” position in order to direct
the light to the video/photo port.
• Remove the protective cap on top of the video/photo port.
• Slip and secure the body of C-mount CCD camera coupler over the video/
photo port, with its focus ring facing back.
• Attach the C-mount video or digital camera (when using cameras with an
integrated lens filter thread of 28, 37 or 43 mm, the Digital Camera Adapter
Kit #501384 is required).
• Refer to the camera’s manual for connecting the camera to monitor.
• Focus the image on the screen by adjusting the fine focus ring located at
the back of the C-mount CCD camera coupler.

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MAINTENANCE
This instrument should be kept in a cool, dry place free from direct sun, dust, alkali
and acid fumes, as well as moisture.
Do not disassemble any of the lenses since they have all been properly aligned at
the factory. The glass surfaces of the lenses should be cleaned with lens tissue or
brushed with a soft camel-hair brush. Any dust which has fallen on lens exterior
surfaces may be blown away with a commercial grade clean dust spray.
If stains deposit on exterior surfaces of the lenses, clean them gently using a mild
soap solution with a lint free cloth. Industrial solvents should not be used since they
may penetrate to the inside of the lens components and dissolve the lens cement.
When cleaning and lubricating the mechanical parts, special attention must be taken
not to stain the optical surfaces with grease. The moveable mechanical parts should
be lubricated occasionally with a non-corrosive grease.
When not in use, remove the eyepieces and put them back into the lens box.
Replace them with the eyepiece tube dust covers. The microscope should then be
secured in its box or covered with a dust cover.

8 World Precision Instruments
ACCESSORIES
502000 PZMIV Binocular Body With 10X Eyepieces, 1X Objective, Eye guards
502001 PZMTIV Trinocular Body With 10X Eyepieces, 1× Objective, Eye
guards
502004 Boom Stand (Heavy) W/O Focus Mount (requires 502009 Focus Mount for
PZMIV)
502005 Ball Bearing Boom Stand (Heavy) W/O Focus Mount (requires 502009
Focus Mount for PZMIV
502006 Boom Clamp Stand (Heavy) (requires 502009 Focus Mount for PZMIV)
504123 Extension for Heavy Clamp Stand
502007 Articulated Arm and Table Clamp w/o Focus Mount (requires 502009
Focus Mount for PZMIV)
502009 Universal Focus Mount for 76 mm PZMIV (Required for BS, AAC, BBS,
and BCS) (5/8” pin)
502160 Lighted Fan Base, Fluorescent base, Tungsten Halogen beam
502010 10× Wide Field Eyepiece for PZMIV (pair)
502011 16× Wide Field Eyepiece for PZMIV (pair)
502012 20× Wide Field Eyepiece for PZMIV (pair)
502013 25× Wide Field Eyepiece for PZMIV (pair)
500264 10× Eyepiece with Reticle (matches 10× eyepiece #502010)
500266 20× Eyepiece with Reticle (matches 20× eyepiece #502012)
502015 Ring Light Adapter for PZMIV (For R-8-8-WPI01 Ring Light Guide)
502016 0.32×, Planachromatic Objective (Distortion-free) (278 mm WD)
502017 0.50×, Planachromatic Objective (Distortion-free) (174 mm WD)
502018 0.63×, Planachromatic Objective (Distortion-free) (138 mm WD)
502019 1.0×, Planachromatic Objective (Distortion-free) (73mm WD)
500261 0.35× CCD Camera Coupler, C-Mount (Use with USBCAM33)
500262 0.5× CCD Camera Coupler, C-Mount (Use with COLCAM, USBCAM50)
500028 1× CCD Camera Coupler, C-Mount (Use with COLCAM)
502163 Wall Mount Plate for Articulated Arm System
NOVA NOVAFLEX Fiber Optic Illuminator
500186 Bifurcated Light Guide with Lenses
R-8-8-WPI01 Ring Light Guide
NOVA-186 NOVAFLEX Fiber Optic Illuminator with Bifurcated Light Guide and
Lenses
EJA Replacement Lamp for NOVAFLEX
502167 Replacement Fluorescent lamp for Lighted Fan Base (502160)
502168 Replacement Tungsten Halogen lamp for Lighted Fan Base (502160)

PZMIV/PZMTIV
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SPECIFICATIONS
This unit conforms to the following specifications:
Eyepieces .................................................................................................................................WFH 10X
Auxiliary Lenses ................................................................................................................................. 1X
Zoom Range....................................................................................................................... 0.62X – 5X
Total Magnification .......................................................................................................... 6.2X – 50X
Zoom Ratio ......................................................................................................................................... 8:1
Field of View ............................................................................................................. ∅33.9- ∅4.2mm
Working Distance .......................................................................................................................80mm
Binocular Tube.................................................................................................................. Inclined 45°
Interpupilary Distance.................................................................................................... 50 – 75mm
Diopter Adjustment........................................................................................................... ±5 Diopter
Microscope Body........................................................................................................Rotatable 360°
Optional Accessories
Eyepieces ...................................................................................................................16X, 20X, 25X
Auxiliary Lenses...............................................................................................0.32X, 0.5X, 0.63X
Total Magnification......................................................................................................1.9X - 125X
Field Of View..........................................................................................................∅106–∅1.8mm
Working Distance ........................................................................................................ 80–296mm
Shipping Weight............................................................................................................................23 lb.
PZMIV & PZMTIV Eyepieces and Objectives
10x Eyepiece 16x Eyepiece 20x Eyepiece 25x Eyepiece
Objective Mag Field (mm)
(Video Field)
Mag Field (mm)
(Video Field)
Mag Field (mm)
(Video Field)
Mag Field (mm)
(Video Field)
Working
Distance
0.32x 1.9x -
16x
106 -13.1
(49.8 - 6.1)
3.2x
25.6x
70.6 - 8.8
(49.8 - 6.1)
3.9x -
32x
55.4 - 6.9
(49.8 - 6.1)
5x -
40x
45.4 - 5.6
(49.8-6.1)
278 mm
0.5x 3.1x -
25x
67.7 - 8.4
(31.8 - 3.95)
5x -
40x
45.2 - 5.6
(31.8 - 3.95)
6.2x -
50x
35.5 - 4.4
(31.8 - 3.95)
7.8x -
62.8x
20 - 3.6
(31.8-3.95)
174 mm
0.63x 3.9x -
31.5x
53.8 - 6.7
(25.3 - 3.15)
6.2x -
50.4x
35.8 - 4.4
(25.3 - 3.15)
7.8x
-63x
28.2 - 3.5
(25.3 - 3.15)
9.8x -
78.8x
23 -2.9
(25.3-3.15)
138 mm
1.0x (inc) 6.2x -
50x
33.9 - 4.2
(15.9 - 1.97)
9.9x -
90x
22.6 - 2.8
(15.9 - 1.97)
12.4x
- 100x
17.7 - 2.2
(15.9 - 1.97)
15.5x -
125x
14.5 - 1.8
(15.9 -.97)
73 mm
The Video Field is based on a 1/2-inch CCD (8 mm diagonal) and a 0.5x camera adapter.

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APPENDIX: BOOM STAND ASSEMBLY
1. Remove all the parts from the box.
2. Unscrew the hex screw from
Vertical Post. The Vertical Post
should have a cross block
attached to it.
3. Screw Vertical Post and Base
together using the hex screw.
(Insert the screw from bottom of
the base into the threaded section
of Vertical Post.)
4. Insert the Horizontal Bar into the
opening in the cross block with the
slot side facing up.
Two different types of focusing assemblies are available:
Focus Mount
1. Remove the focus mount from the protective packaging
2. Insert the focus mount into the end
of the Horizontal Bar and secure it by
tightening set screw.
3. Tighten one or both hex screws (2.5
mm and 4 mm) so the microscope
head is tiltable but not free to swing.
TIP: The focus mount can be inserted
from the TOP, BOTTOM or END of the
Horizontal Bar. For oblique viewing, set
the focus mount up at various angles and then tighten the handle.

PZMIV/PZMTIV
World Precision Instruments 11
* Electrodes, batteries and other consumable parts are warranted for 30 days only from the date on which the
customer receives these items.
WARRANTY
WPI (World Precision Instruments, Inc.) warrants to the original purchaser that this equipment,
including its components and parts, shall be free from defects in material and workmanship for a
period of one year* from the date of receipt. WPI’s obligation under this warranty shall be limited
to repair or replacement, at WPI’s option, of the equipment or defective components or parts upon
receipt thereof f.o.b. WPI, Sarasota, Florida U.S.A. Return of a repaired instrument shall be f.o.b.
Sarasota.
The above warranty is contingent upon normal usage and does not cover products which have been
modified without WPI’s approval or which have been subjected to unusual physical or electrical stress
or on which the original identification marks have been removed or altered. The above warranty will
not apply if adjustment, repair or parts replacement is required because of accident, neglect, misuse,
failure of electric power, air conditioning, humidity control, or causes other than normal and ordinary
usage.
To the extent that any of its equipment is furnished by a manufacturer other than WPI, the foregoing
warranty shall be applicable only to the extent of the warranty furnished by such other manufacturer.
This warranty will not apply to appearance terms, such as knobs, handles, dials or the like.
WPI makes no warranty of any kind, express or implied or statutory, including without limitation any
warranties of merchantability and/or fitness for a particular purpose. WPI shall not be liable for any
damages, whether direct, indirect, special or consequential arising from a failure of this product to
operate in the manner desired by the user. WPI shall not be liable for any damage to data or property
that may be caused directly or indirectly by use of this product.
Claims and Returns
Inspect all shipments upon receipt. Missing cartons or obvious damage to cartons should be noted
on the delivery receipt before signing. Concealed loss or damage should be reported at once to the
carrier and an inspection requested. All claims for shortage or damage must be made within ten
(10) days after receipt of shipment. Claims for lost shipments must be made within thirty (30) days
of receipt of invoice or other notification of shipment. Please save damaged or pilfered cartons until
claim is settled. In some instances, photographic documentation may be required. Some items are
time-sensitive; WPI assumes no extended warranty or any liability for use beyond the date specified
on the container
Do not return any goods to us without obtaining prior approval and instructions from our Returns
Department. Goods returned (unauthorized) by collect freight may be refused. Goods accepted for
restocking will be exchanged or credited to your WPI account. Goods returned which were ordered
by customers in error are subject to a 25% restocking charge. Equipment which was built as a special
order cannot be returned.
Repairs
Contact our Customer Service Department for assistance in the repair of apparatus. Do not return
goods until instructions have been received. Returned items must be securely packed to prevent
further damage in transit. The Customer is responsible for paying shipping expenses, including
adequate insurance on all items returned for repairs. Identification of the item(s) by model number,
name, as well as complete description of the difficulties experienced should be written on the repair
purchase order and on a tag attached to the item.

World Precision Instruments, Inc.
USA
International Trade Center, 175 Sarasota Center Blvd., Sarasota FL 34240-9258
Tel: [phone] • Fax: [phone] • E-mail: [e-mail]
UK
1 Hunting Gate, Hitchin, Hertfordshire SG4 0TJ
Tel: [phone] • Fax: [phone] • E-mail: [email protected]
Germany
Zossener Str. 55, 10961 Berlin
Tel: [phone] • Fax: [phone] • E-mail: [email protected]
China & Hong Kong
WPI Shanghai Trading Co., Ltd.
Rm 20a, No8 Dong Fang Rd., Lu Jia Zui Financial District, Shanghai PRC
Tel: [phone] • E-mail:chinas[email protected].com
Internet
www.wpiinc.com • store.wpiinc.com • www.wpichemistry.com
www.wpi-europe.com • www.wpiinc.cn