WPI INV-101 User manual

INSTALL GUIDE
Serial No._____________________
102811
World Precision Instruments
www.wpiinc.com
INV-101
Inverted Microscope

INV-101
II WORLD PRECISION INSTRUMENTS

INV-101
WORLD PRECISION INSTRUMENTS iii
Copyright © 2011 by World Precision Instruments, Inc. All rights reserved. No part of this publication may be
reproduced or translated into any language, in any form, without prior written permission of World Precision
Instruments, Inc.
CONTENTS
ABOUT THIS MANUAL ............................................................................................................................... 1
INTRODUCTION............................................................................................................................................ 2
Unpacking ................................................................................................................................................. 2
MICROSCOPE SETUP................................................................................................................................... 3
Setting up the Base and Stage ........................................................................................................... 3
Installing the Condenser and Lamp Housing................................................................................ 7
Setting up the Stage and Phase Aperture....................................................................................... 9
Installing the Trinocular Head Assembly........................................................................................ 9
Installing the Objectives .....................................................................................................................11
Installing Adapter Plates, Eyepieces and Filters..........................................................................13
Phase Contrast Alignment .................................................................................................................17
Adjusting the Phase Contrast Alignment................................................................................17
Mounting a Camera .............................................................................................................................19
Direct C-Mount on a Threaded Triangular Attachment.....................................................20
Vertical Eye Tube Mounting.........................................................................................................21
Eyepiece Mounting.........................................................................................................................21
Eyepiece Plus C-Mount Mounting ...................................................................................................22
BASIC MICROSCOPE OPERATIONS ......................................................................................................23
Camera Shutter (Optical) Path...........................................................................................................23
Coaxial X-Y Control..............................................................................................................................23
Micrometer Focus Control..................................................................................................................24
Focus Tensioning Adjustments.........................................................................................................24
INDEX .............................................................................................................................................................27
WARRANTY ..................................................................................................................................................28
Claims and Returns ..............................................................................................................................28

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WORLD PRECISION INSTRUMENTS 1
ABOUT THIS MANUAL
The following symbols are used in this guide:
This symbol indicates a CAUTION. Cautions warn against actions that can cause
damage to equipment. Please read these carefully.
This symbol indicates a WARNING. Warnings alert you to actions that can cause
personal injury or pose a physical threat. Please read these carefully.
NOTES and TIPS contain helpful information.
Fig. 1–INV-101 Inverted microscope

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INTRODUCTION
This document details the assembly of the INV-101 inverted microscope. It includes
optional installation of a camera. All cameras and mounting adapters are sold separately.
Unpacking
Upon receipt of this instrument, make a thorough inspection of the contents and check
for possible damage. Missing cartons or obvious damage to cartons should be noted on
the delivery receipt before signing. Concealed damage should be reported at once to the
carrier and an inspection requested. Please read the section entitled “Claims and Returns”
on page 28 of this manual. Please contact WPI Customer Service if any parts are
missing at 941.371.1003 or [e-mail].
Returns: Do not return any goods to WPI without obtaining prior approval (RMA #
required) and instructions from WPI’s Returns Department. Goods returned (unauthorized)
by collect freight may be refused. If a return shipment is necessary, use the original
container, if possible. If the original container is not available, use a suitable substitute
that is rigid and of adequate size. Wrap the instrument in paper or plastic surrounded with
at least 100mm (four inches) of shock absorbing material. For further details, please read
the section entitled “Claims and Returns” on page 28 of this manual.
Fig. 2–Contents of the two boxes

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MICROSCOPE SETUP
Before setting up the microscope, carefully open the box and inspect its contents. The
pieces of the microscope are stored in two Styrofoam containers, and many of the
pieces are wrapped in plastic (Fig. 2). Carefully unwrap each piece before setting up the
instrument. The following instructions graphically show how to setup your inverted
microscope.
NOTE: If you have a problem/issue that falls outside the definitions of this manual,
contact the WPI Technical Support team at 941.371.1003 or technicalsupport@wpiinc.
com.
Setting up the Base and Stage
1. Remove the base of the microscope and set it up on a firm tabletop surface (Fig. 3).
Fig. 4 shows the back of the microscope base. The A/C power plug adapter, the serial
number and the fuse compartment are located on the back. Fig. 5 shows the power
switch and lamp brightness control located on the side of the microscope.
Condenser Mount
Lamp Connector
Head Mount
Stage
5-Position Nosepiece
Focus Control Knob
FrontBack
Fig. 3–Base of the microscope.

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Fig. 4–Back of the microscope base showing power connection, serial number and fuse
compartment
Fig. 5–Power switch and lamp brightness control on the side of the microscope
2. Locate the coaxial stage assembly. It is shown in Fig. 6. The captive mounting screws
are located on the side of it (Fig. 7).
Fig. 6–Coaxial stage assembly

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Fig. 7–Captive mounting screws on the coaxial stage assembly
3. Tip the microscope base over on its left side to reveal the location of the screw
mounts. Then, position the coaxial stage (Fig.8). Fig. 9 shows a close up of the captive
screws.
Fig. 8–Microscope on its side Fig. 9–Close up of captive mounting screws
4. With a flat head screwdriver, gently tighten the captive screws (Fig. 10).
Fig. 10–Gently tighten the captive mounting screws.

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5. Locate the left extension stage plate bottom shown in Fig. 11 (bottom view) and Fig.
12 (top view).
Fig. 11- Extension stage plate (bottom view) Fig. 12–Extension stage plate (top view)
6. Push extension arm of the coaxial stage assembly inward so that you do not damage
it when you mount the left extension stage plate (Fig. 13).
Fig. 13–Extension arm of coaxial stage assembly.
7. Then, with the microscope base laying on its right side, locate the captive screws and
mounting holes for left stage plate (Fig. 14) and install the left stage plate (Fig. 15).
Fig. 14–Left stage plate captive screws Fig. 15–Plate installed viewed from below

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8. Turn the microscope upright (Fig. 16).
Fig. 16–Coaxial and left stage plate mounted
Installing the Condenser and Lamp Housing
1. Locate the condenser assembly and lamp housing (Fig. 17).
Condenser
Assembly
Lamp Housing
Fig. 17–Condenser assembly and lamp housing
2. Lower the condenser assembly into the condenser mount (Fig. 18). It should slide
easily into place. (Note the orientation.) Then, rotate the condenser assembly 90º
counter-clockwise into position (Fig. 19).
Fig. 18–Condenser in mounting position Fig. 19–Condenser rotated 90º CCW

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3. Tighten hex screws to secure the condenser assembly into the condenser mount (Fig. 20).
Fig. 20–Tighten hex screws on condenser head
4. Plug the lamp housing into the lamp connector (Fig. 21).
Fig. 21–Connect the lamp Fig. 22–Lamp connected Fig. 23–Install lamp
5. Lower the lamp housing onto the mounting pin sockets in the top of the condenser
head. (Fig. 23). The assembled condenser and lamp housing is shown in Fig. 24.
Fig. 24–Assembled condenser with lamp housing

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Setting up the Stage and Phase Aperture
1. Position the clear stage plate in the recess on the stage (Fig. 25).
Fig. 25–Clear stage plate, installed
2. Install the phase aperture frame (Fig. 26) in the slot on the condenser head from the
right side. Orient the phase aperture frame as shown in Fig. 27.
10X/20X/40X
Condenser
Annulus
Filter
Frame
Fig. 26–Phase aperture frame Fig. 27–Install the phase aperture frame
Installing the Trinocular Head Assembly
1. Remove the dust cover from the trinocular head assembly (Fig. 28) and the head
mount (Fig. 29).
Fig. 28–Trinocular head and dust cover Fig. 29–Cover on head mount of microscope base

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2. Place the trinocular head on the head mount and tighten the side screw (Fig. 30).
Fig. 30–Position head on the mount
3. Locate the threaded C-mount, eye tube mount and trinocular extension tube (Fig. 31).
1X Threaded
C-Mount
Eye Tube
Mount
Trinocular
Extension Tube
Fig. 31–C-mount, eye tube mount and trinocular tube extension
4. Remove the trinocular extension tube mount dust cover from the trinocular head (Fig.
32) and place the extension tube into the opening (Fig. 33). Tighten the locking screw
on the side.
Fig. 32–Remove dust cover Fig. 33–Position extension tube

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5. Depending on your application, either the eye tube mount or the C-mount can be
installed. Choose one of the two options.
• Mount the eye tube mount by inserting it into the extension tube (Fig. 34).
• Mount the threaded C-mount by screwing it into the extension tube (Fig. 35).
2X C-Mount Here
Fig. 34–Eyepiece tube screw mounted Fig. 35–Threaded C-mount mounted
Installing the Objectives
Four objectives are included with the microscope. They are in protective shipping
tubes with captive dust covers (Fig. 36). The nosepiece allows you to mount up to five
objectives. The nosepiece (for mounting the objectives) is shown in Fig. 37. (The clear
plate was removed for this part of the installation.) When mounting the objectives,
remove the nosepiece dust covers individually when you are ready to install an objective.
Leave the dust covers in place on unused mounting holes.
Fig. 36–Objective, pictured as shipped Fig. 37–5-Position nosepiece

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1. Remove a single dust cover from the nosepiece (Fig. 38). When it is removed, an
opening is revealed (Fig. 39). For ease of installation, that opening should point
straight up through the opening on the microscope stage.
Fig. 38–Removing dust cover to mount objective Fig. 39–Position for mounting objective
2. Remove the desired objective from its shipping case (Fig. 40). Then, remove the cap-
tive dust cover (its cap) (Fig. 41).
Fig. 40–Objective out of case Fig. 41–Objective with cap removed
3. Insert the objective into the nosepiece and rotate it clockwise. Finger tighten it
(Fig.42). DO NOT OVERTIGHTEN. Finger tight is sufficient!
Fig. 42–Installing the objective

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4. Rotate the nosepiece to access next mounting location (Fig. 43).
Fig. 43–Rotated nosepiece Fig. 44–Four objectives mounted
5. Repeat steps 1-4 to install the remaining objectives. Do not remove the dust covers
until you are ready to install the next objective. This prevents any stray dust from be-
ing trapped on the objective. The final assembly is shown in Fig. 44. The objectives
are arranged in order (4X, 10X, 20X, 40X). An optional fifth objective location is also
provided. If a fifth objective is not installed, leave the dust cover in place.
6. Re-position the clear stage plate in the recess on the stage (Fig. 45).
Fig. 45–Clear stage plate in place
Installing Adapter Plates, Eyepieces and Filters
1. Locate the 100mm round stage mount, rectangular plate and 35mm insert (Fig. 46).
50mm/1x3” Slide
Adapter
Rectangular
Lab Jar Adapter 35mm Dish
Adapter
Fig. 46–100mm round stage mount, rectangular plate and 35mm insert

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2. Insert the 35mm adapter plate into the rectangular plate (Fig. 47).
Fig. 47–Assembled rectangular plate and 35mm insert
3. Place the rectangular plate on the stage plate (Fig 48).
Fig. 48–Placement location of the assembled stage plate
4. Remove the eyepieces from the dust wraps (Fig. 49) and remove the dust covers from
the eyepiece openings on the trinocular head (Fig. 50).
Fig. 49–Eyepieces Fig. 50–Remove dust covers from trinocular
head

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5. Place the eyepieces in the microscope ocular openings (Fig 51). Adjust the diopter set-
ting to zero.
Fig. 51–Placement of eyepieces
6. Locate the filters (Fig. 52).
Fig. 52–Filters
7. Remove the filter holder from the condenser head as shown in Fig. 53 and Fig. 54.
Fig. 53–Filter holder position Fig. 54–Filter holder removed

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8. Place the desired filter in the filter holder (Fig. 55) and slide the holder back into the
condenser head (Fig. 56 and 57).
Fig. 55–Insert filter Fig. 56–Position holder Fig. 57–Slide holder in place
TIP: If desired, the filter may alternatively be placed in the condenser annulus slider (Fig.
58) and slid into position for use (Fig. 59).
Fig. 58–Filter in condenser annulus slider Fig. 59–Condenser annulus slider in position