WPI PZMIV User manual

PZMIV & PZMTIV
Precision Zoom Stereo Microscopes
INSTRUCTION MANUAL
Serial No._____________________
072105
World Precision Instruments
www.wpiinc.com

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 11
Contents
Introduction ............................................................................................................. 1
Unpacking and Set-Up............................................................................................ 2
Parts Diagram for PZMIV and PZMTIV.................................................................... 3
PZMTIV System Diagram ........................................................................................ 4
Specifications.......................................................................................................... 5
Operation................................................................................................................. 6
Maintenance............................................................................................................ 7
Appendix: Boom Stand Assembly .......................................................................... 8
Warranty .................................................................................................................. 9
Copyright © 2004 by World Precision Instruments, Inc. All rights reserved. No part of this publication
may be reproduced or translated into any language, in any form, without prior written permission of
World Precision Instruments, Inc.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 1
Introduction
WPI’s PZMIV stereomicroscope is an ideal tool for tissue dissection, cell injection,
specimen manipulation, electrode inspection and many other applications
requiring a magnified stereo view and ample working distance. It offers quality and
performance at an affordable price. Advanced optics provide image sharpness
found only among the best of this class.
PZMIV uses modular, parallel-beam path design and a high quality optical system.
The advanced optical design with plan achromatic objectives provides sharp and
distortion-free image throughout the entire zoom range. The ergonomic design
makes it easy to use — zooming is smooth and effortless, controlled by a large
spring-loaded knob.
With an extensive array of optional accessories, PZMIV is suitable for almost any
bio-research application requiring a stereomicroscope.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 2
Unpacking and Set-Up
1. Remove the microscope from the case and check that the following parts are
all present and undamaged:
Microscope Head
WFH 10x Wide-Field Eyepieces (two)
Paired Eyeguards (two)
Ring Light Adapter
Track Stand
2. Assemble the track stand.
3. Mount the microscope head into the focus mount.
4. Remove the protective cap on the bottom of the microscope head.
5. Refer to the parts diagram on page 3, and follow the set-up instructions
below.
6. Install the WFH 10x eyepiece pair (1) included. The paired eyeguards can
now be attached to the eyepieces.
7. The exact working distance is found by adjusting the focusing knob (7).
8. When the optional stands are used instead of the standard base, see
System Diagram on page 4 for mounting instructions.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 3
Parts Diagram for PZMIV and PZMTIV
Key
(1) Eyepiece
(2) Diopter Adjustment
Ring
(3) Magnification
Indicating Ring
Above the right-
hand knob (hidden
in this photo) is a
detent control to
provide discreet
zoom stops or
continuous zooming.
(4) Magnification
Knob
(5) Body Rotation
Clamp Locking
Screw
(6) Stage Plate
(7) Focusing
Knob
(8) Track
(9) Base
PZMTIV only:
(10) CCD Color Video
Camera (optional)
(11) C-Mount CCD
Camera Coupler
(optional)
(12) Video/Photo Port
(13) Light Path Selector
Bar — when
out, light to the
right eyepiece is
switched to the
photo port.
10
11
Fig. 1
1
2
3
8
9
7
4
5
6
12
13

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 4
PZMTIV
System
Diagram
Universal Focus Mount
#502009
Boom Stand
#502004
Articulated Arm
with Table Clamp: #502007
optional Wall Mount: #501366
Ball-Bearing Stand
#502005
Post Stand
#502003
Objectives:
0.32x — #502016
0.5x — #502017
0.63x — #502018
1.0x — #502019 *
Eyepieces:
10x — #502010 *
16x — #502011
20x — #502012
25x — #502013
Digital camera (with integrated
lens filter thread of 28, 37 or
43 mm)
Digital Camera
Adapter Kit
#501384
Video Camera
#COLCAM
CCD Coupler
0.3x — #500261
0.5x — #500262
0.75x — #500263
Ring Light Adapter
#502015 *
Pixera
#VCS-10132
Track Stand
#502002
Binocular Body
#502000
Trinocular Body
#502001
*Included with body.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 5
Specifications
EYEPIECES......................................... WFH 10x (two), standard;
............................................................ WF 15x, 20x, 25X, optional
OBJECTIVE ........................................ 1x
OBJECTIVES (optional)...................... 0.3x, 0.4x, 0.5x, 0.75x, 1.5x, 2x
ZOOM RANGE ................................... 0.62x - 5x
TOTAL MAGNIFICATION.................... 6.2x - 50x
FIELD OF VIEW .................................. Ø 33.9 MM - Ø 4.2 MM
WORKING DISTANCE ........................80 mm
BINOCULAR TUBE............................. Inclined 45°
INTERPUPILLARY DISTANCE ............ Adjustable 50-75 mm
DIOPTER ADJUSTMENT.................... ±5 Diopter (both eyepiece tubes)
MICROSCOPE BODY......................... Rotatable 360°
AUXILIARY LENSES
Total magnification.....................1.9x - 125x
Biggest Field of View ................. Ø 106 mm
Working Distance.......................80-296 mm
PZMIV & PZMTIV Eyepieces and Objectives
10x Eyepiece 16x Eyepiece 20x Eyepiece 25x Eyepiece
Objective Mag Field (mm)
(Video Field) Mag Field (mm) (Video Field) Mag Field (mm) (Video Field) Mag Field (mm) (Video Field)
Working
Distance
0.32x 1.9x - 16x 106 -13.1
(49.8 - 6.1) 3.2x 25.6x 70.6 - 8.8 (49.8 - 6.1) 3.9x - 32x 55.4 - 6.9 (49.8 - 6.1) 5x - 40x 45.4 - 5.6 (49.8 - 6.1) 296 mm
0.5x 3.1x - 25x 67.7 - 8.4
(31.8 - 3.95) 5x - 40x 45.2 - 5.6 (31.8 - 3.95) 6.2x - 50x 35.5 - 4.4 (31.8 - 3.95) 7.8x - 62.8x 20 - 3.6 (31.8 - 3.95) 187 mm
0.63x 3.9x - 31.5x 53.8 - 6.7
(25.3 - 3.15) 6.2x - 50.4x 35.8 - 4.4 (25.3 - 3.15) 7.8x -63x 28.2 - 3.5 (25.3 - 3.15) 9.8x - 78.8x 23 -2.9 (25.3 - 3.15) 149 mm
1.0x (inc) 6.2x - 50x 33.9 - 4.2
(15.9 - 1.97) 9.9x - 90x 22.6 - 2.8 (15.9 - 1.97) 12.4x - 100x 17.7 - 2.2 (15.9 - 1.97) 15.5x - 125x 14.5 - 1.8 (15.9 - 1.97) 80 mm
The Video Field is based on a 1/2-inch CCD (8 mm diagonal) and a 0.5x camera adapter.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 6
Operation
Refer to Figure 1 on page 3 and system diagram on page 4 along with the
following instructions on normal operation.
1. Set Up Microscope on Track Stand
Mount the microscope head into the focus mount. Use focusing knob to bring
microscope to focus.
2. Interpupilary Distance Adjustment
Looking through the eyepieces with both eyes, adjust the interpupillary
distance by sliding the two eyepiece tubes with both hands until a single
image is observed.
3. Focus Adjustment
Place a sample under the microscope, turn magnification knob (4) to its
greatest setting (5.0). Focus the image by turning the focusing knob (7).
Once image is in clear focus at its highest magnification you may zoom out
to lower magnification by adjusting magnification knob (4) while remaining in
focus.
4. Diopter Adjustment
If image does go out of focus when zooming out, zoom to the lowest setting
and focus each eyepiece individually by adjusting the diopter adjustment ring
(2).
5. Video/Photo Applications
Slide the light path selector bar (13) to the “PHOTO IN” position in order
to direct the light to the video/photo port (12). Remove the protective cap
on top of the video/photo port. Slip and secure the body of C-mount CCD
camera Coupler (11) over the video/photo port, with its focus ring facing
back. Attach C-mount video or digital camera (when using cameras with an
integrated lens filter thread of 28, 37 or 43 mm, Digital Camera Adapter Kit
#501384 is required). Refer to camera’s manual for connecting camera to
monitor. Focus image on screen by adjusting the fine focus ring located at the
back of the C-mount CCD camera coupler (11).

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 7
Maintenance
This instrument should be kept in a cool, dry place free from direct sun, dust, alkali
and acid fumes as well as moisture.
Do not disassemble any of the lenses since they have all been properly aligned
at the factory. The glass surfaces of the lenses should be cleaned with lens tissue
or brushed with a soft camel-hair brush. Any dust which has fallen on lens exterior
surfaces may be blown away with a commercial grade clean dust spray.
If stains deposit on exterior surfaces of the lenses, clean them gently using a mild
soap solution with a lint free cloth. Industrial solvents should not be used since they
may penetrate to the inside of the lens components and dissolve the lens cement.
When cleaning and lubricating the mechanical parts, special attention must be
taken not to stain the optical surfaces with grease. The moveable mechanical parts
should be lubricated occasionally with a non-corrosive grease.
When not in use, remove the eyepieces and put them back into the lens box.
Replace them with the eyepiece tube dust covers. The microscope should then be
secured in its box or covered with a dust cover.

PZMIV & PZMTIV
WORLD PRECISION INSTRUMENTS 8
Appendix: Boom Stand Assembly
1. Remove all parts from box
2. Unscrew hex screw from Vertical Post. Vertical post
should have cross block attached to it.
3. Screw Vertical Post and Base together using hex screw.
(Insert screw from bottom of base, into threaded section
of vertical post)
4. Insert Horizontal Bar into opening in cross block, slot side
facing up.
Two different types of focusing assemblies are available:
E-arm
1. Remove E-arm from protective packaging
2. Insert E-arm into the end of the Horizontal Bar, secure by
tightening set screw.
3. Tighten one or both hex screws (2.5 mm and 4 mm) so the microscope head is
tiltable but not free to swing.
Tips
E-arm can be inserted from the TOP, BOTTOM or END of the Horizontal Bar.
For oblique viewing, set E-arm up at various angles and then tighten handle.