Yamato SM300 User manual

High Pressure Steam Sterilizer
SM200 SM300
SM310 SM510
Second Edition
● Thank you for your Yamato Scientific RE series Rotary Evaporator purchase
● For best test data, we recommend you purchase our BM series Water Bath.
Please call Yamato Scientific for more details.
Yamato Scientific America Inc.
Santa Clara, CA
[phone]
Read and apprehend the important warnings in
this instruction manual prior to use.

Table of Contents
1. Specifications.................................................................................................................................1-2
2. Safety Information..........................................................................................................................3-7
Safety Symbols.................................................................................................................................... 3
Safety Precautions ...........................................................................................................................4-6
Hazardous Material ............................................................................................................................. 7
3. Identification of Parts...................................................................................................................8-11
External................................................................................................................................................ 8
Internal............................................................................................................................................9-10
Control Panel..................................................................................................................................... 11
4. Installation...................................................................................................................................12-18
Environmental Requirements.......................................................................................................12-13
Power Requirements......................................................................................................................... 14
Initial Set Up .................................................................................................................................15-18
5. Operational Precautions............................................................................................................19-20
6. Sample Run Examples.................................................................................................................... 21
7. Program “MODE” .......................................................................................................................22-27
“MODE” key Information.................................................................................................................... 22
Display Symbols................................................................................................................................ 23
Flowchart........................................................................................................................................... 24
Setting Sterilization Temperature and Time...................................................................................... 25
Setting Dry Temperature and Time................................................................................................... 26
Special Program Features................................................................................................................. 27
8. Run “MENU” ...............................................................................................................................28-33
Selection of Operation....................................................................................................................... 28
“STERILIZE” Procedure..............................................................................................................29-30
“STERILIZE & DRY” Procedure .................................................................................................31-33
9. Special Control Functions.........................................................................................................34-35
View Parameter during Operation..................................................................................................... 34
Interrupt Program during Operation .................................................................................................. 35
Pressure Relief Switch ...................................................................................................................... 35
Checking chamber temperature during stand-by mode.................................................................... 35
10. Troubleshooting Guide............................................................................................................36-37
Safety................................................................................................................................................. 36
Self-diagnostic Circuitry..................................................................................................................... 36
Error Code List .................................................................................................................................. 36
In case of power failure ..................................................................................................................... 36
Problem Solving Chart....................................................................................................................... 37
11. Wiring Diagram.........................................................................................................................38-39
12. Piping Chart................................................................................................................................... 40
13. Maintenance..............................................................................................................................41-42
Daily Maintenance............................................................................................................................. 41
Weekly Maintenance......................................................................................................................... 41
Monthly Maintenance ........................................................................................................................ 42
14. After Sale Service and Warranty.................................................................................................. 43
Request for Repair ............................................................................................................................ 43
15. Replacement Parts List ................................................................................................................ 44

1
Specifications
SM200/300
Model SM200 SM300
System Automatic high pressure steam sterilizer
Sterilization 105℃〜123℃105℃〜128℃ Temperature
Setting Range Drying 150℃〜180℃
Maximum Operational Pressure 0.18MPa (26.1psi) 0.2MPa (29.0psi)
Power Requirement AC115V 13A (50/60Hz) AC115V 17A (50/60Hz)
Controller HiTec IV CR Type Microprocessor Control
Sensor 1 (Chamber Temp.) Pt100Ωresistance thermometer sensor
Sensor 2 (Water Temp.) Thermocouple (Type T)
Timer 1min. 〜99hours and 59min. 100 〜999hours
Heater 1 (Sterilization) 1.3kW 1.7kW
Heater 2 (Drying) 1.0kW 1.5kW
Safety
Main circuit breaker, Over pressure safety valve,
Self-diagnostic circuitry; Monitors any abnormality of the temp sensor,
Low water sensor, SSR, and heater
External Dimensions (W×D×H): 16.1”×18.5”×37.4”
41cm×47cm×95.3cm
17.3”×20.9”×38.0”
44cm×53cm×96.5cm
Internal Dimensions
(Diameter, Depth):
9.4”, 17.5”
24cm, 44.5cm
11.8”, 17.5”
30cm, 44.5cm
Capacity (cu. ft): 0.8 1.2
Capacity (liters): 22 36
Weight 143.3 pounds
65kg
176.4 pounds
80kg
Accessories
Bottle 1
Basket 2
Bottom Plate 1
Steam Saucer １
Instruction Manual 1
Warranty 1

2
Specifications
SM310/510
Model SM310 SM510
System Automatic High pressure steam sterilizer
Sterilization 105℃〜128℃ Temperature
Setting Range Drying 150℃〜180℃
Maximum Operational Pressure 0.2MPa (29.0psi)
Power Requirement AC220V 11A (50/60Hz)
Controller HiTec IV CR Type Microprocessor Control
Sensor 1 (Chamber Temp.) Pt100Ωresistance thermometer sensor
Sensor 2 (Water Temp.) Thermocouple (Type T)
Timer 1min. 〜99hours and 59min. 100 〜999hours
Heater 1 (Sterilization) 2.0kW
Heater 2 (Drying) 1.5kW
Safety
Main circuit breaker, Over pressure safety valve,
Self-diagnostic circuitry; Monitors any abnormality of the temp sensor,
Low water sensor, SSR, and heater
Intend Dimensions (W×D×H): 17.3”×20.9”×38.0”
44cm×53cm×96.5cm
17.3”×20.9”×42.7”
44cm×53cm×108.5cm
Internal Dimensions
(Diameter, Depth):
11.8”, 17.5”
30cm, 44.5cm
11.8”, 26.2”
30cm, 66.5cm
Capacity (cu. ft): 1.2 1.8
Capacity (liters): 36 51
Weight 176.4 pounds
80kg
187.4 pounds
85kg
Accessories
Bottle 1
Basket 2
Bottom Plate 1
Steam Saucer １
Instruction Manual 1
Warranty 1

3
Safety Information
Safety Symbols
Safety Information
This instruction manual contains various symbols and safety information. Ignoring safety information
can cause such situations as listed below. Please read the following warning and caution signs in this
manual prior to use.
Warning Indicates the possibility of serious or fatal injury. (Note 1)
Caution Indicates the possibility of injury (Note 2) or damage (Note 3) to the
equipment.
(Note 1) Serious injury: Bodily harm by electric shock, bone fracture or poisoning which may require
hospitalization.
(Note 2) Injury: Bodily harm by electric shock, bone fracture or poisoning which may require
hospitalization.
(Note 3) Damage: Any damage on equipment, facility, structure, etc.
Meaning of graphic indications
Shows warnings or caution.
Specific contents are described aside each sign.
Shows users important information.
Specific contents are described aside each sign.
Shows users important information.
Specific contents are described aside each sign.

4
Safety Information
Safety Precautions
Do not use this unit for any purpose other than its intended use, described in this manual.
This unit is not explosion proof. Never use in flammable or explosive gas environments.
Be sure to ground the unit. Electric leak could cause electrical shock or fire.
Be sure to use a power supply with more than the rated capacity specified in this manual. Use
of a power supply without the correct rated voltage and current could cause fire or electric
shock.
If smoke or any strange odor should disburse from this unit, turn the breaker off immediately
and pull out the main power cord. Then contact Yamato Scientific. Neglecting this procedure
can result in fire or electric shock. Never try repairing the unit yourself.
Forcibly bending, pulling, wrenching or extending the power cord can cause a fire or electric
shock.
Overheat or fire can occur if the power cord is bundled or if an object is on the cord.
The use of explosive, flammable or such compounds can cause explosion or fire. (See Pg.7)
Disassembling this unit can cause fire, electric shock or other crisis’s.
The exhaust is located on the right side of the unit. Possibility of personal injury if you come in
contact with this area during operation or for a period of time after use.

5
Safety Information
Safety Precautions
Do not plug the exhaust. Any obstruction in the exhaust may cause the tank to build pressure
and become hotter the normal.
Do not attempt to open the sterilizer lid until the pressure is at “0psi”.
If you attempt to open the lid when pressurized high pressure steam could
cause serious bodily injury. Do not stand close to the lid since steam will
exhaust once opened.
Do not open drain when vessel is pressurized. Since sterilization water is hot just after
sterilization, let the water cool before draining.
The bottle, located in the inside of the front door contains hot water after operation. Let the
water cool prior to removing the bottle. To avoid possible bodily injury, do not open the door
during operation.
The plate, lid and surrounding areas are hot after operation. To avoid bodily injury, do not touch
these areas. Always wear protective gear when removing a processed load.
Once the lid is open and all steam has exhausted remove sterilized material with heat-resistant
gloves to prevent bodily injury.
Hot steam will disperse out of the louvers located in the back of this unit if a malfunction
occurs. Do not close the louvers.
When using the pressure relief switch be sure not to touch the exhaust areas of the sterilizer
since high pressure steam will exhaust.
Do not operate this unit without a basket.

6
Safety Information
Safety Precautions
In the event of electrical storm turn off the main circuit breaker. Neglecting this procedure can
result in fire, electric shock or other troubles due to thunderbolts.
In the event of a power interruption or the main circuit breaker is switched off during operation
the sterilizer solenoid exhaust valve will remain closed once power has been restored. The
solenoid exhaust valve will not open until the chamber temperature reaches below boiling
point.
Do not attempt to open the sterilizer lid until the pressure is at 0psi.
Use the pressure relief switch to reduce the pressure inside the chamber to “0psi”. (refer to
page 35 for instruction how to use the pressure relief switch)

7
Safety Information
Hazardous Material
The following material is not recommended for use on the sterilizer
Nitroglycol, Nitroglycerin, Nitrocellulose and other explosive nitric
esters.
Trinitrobenzene, Trinitrotoluene, Picric acid, and other explosive
nitro compounds.
Peracetic acid, Methyl ethyl ketone peroxide, Benzoyl peroxide and
other organic peroxides.
Explosive Explosive
Substance
Sodium azide and any other metallic azide compound.
Combustible
Substance
Metallic lithium, Metallic potassium, Metallic sodium, Yellow
phosphorus, Phosphorus sulfide, Red phosphorus, Celluloid,
Calcium carbide(Carbide), Phosphide lime, Megnesium powder,
Aluminum powder, other combustible metal powders and Sodium
dithionite (Hydrosulfite).
Potassium chlorate, Sodium chlorate, Ammonium chlorate and other
perchlorate.
Potassium perchlorate, Sodium perchlorate, Ammonia perchlorate,
and other perchlorates.
Potassium peroxide, Sodium peroxide, Barium peroxide and other
inorganic peroxides.
Potassium nitrate, Sodium nitrate, Ammonia nitrate and other
nitrates.
Sodium chlorite and other chlorites.
Oxidant
Calcium hypochlorite and other hypochlorites.
Ethyl ether, Gasoline, Acetaldehyde, Propylene chloride, Carbon
disulfide and other substance with the flash point below minus 30ºC.
Normal hexane, Ethylene oxide, Acetone, Benzene, Methyl ethyl
ketone and any other substance with the flash points not lower than
minus 30ºC and below 0oC.
Methanol, Ethanol, Xylene, Pentyl acetate (Amyl acetate) and other
substance with the flash point not lower than 0ºC and below 30ºC.
Ignitable
Substance
Kerosene oil, Light oil, Turpentine oil and Isopentyl alcohol(Isoamyl
alcohol), Acetic acid and other substances with the flash point not
lower than 30ºC and below 65ºC
Flammable
Combustible
Gas Hydrogen, Acetylene, Ethylene, Methane, Ethane, Propane, Butane and other flammable gas at 15ºC and under 1 atmosphere.

8
Identification of Parts
External
Handle
Lid
Exhaust (U-shaped Pipe)
Control Panel
A
rm
Pressure Gauge
Door Latch
Bottle Water Window Viewer
Panel Guard
Pressure Relief Switch
Circuit Breaker
Terminal (Optional)
Power Cord
Louver
Louver
Louver
Steam Saucer

9
Identification of Parts
Internal

Sensor 1
Sensor 2
Heater 1
Safety Valve
Thermal Insulato
r
Solenoid Drain Valve
Solenoid Exhaust Valve
Bottom Plate
Heater 2
Chamber
Service Plug
Micro Switch
Filte
r
(For Drying)
(For Water Temperature)
(For Sterilization) (For Chamber Temperature)

10
Identification of Parts
Internal
Plug
Silence
r
Drain Valve
Micro Switch
Exhaust Hose
Heater 1 (For Sterilization)
Filter
Solenoid Exhaust Port
Sensor 1 (For Chamber Temperature)
Sensor 2
(For Water Temperature)
Flange
Clamp

11
Identification of Parts
Control Panel
①ENTER key Start and stop operation, fix the set point/function changed.
②△▽(up down) key Change set point/function.
③MENU key Choose “STERILIZE” or “STERILIZE & DRY” before operation.
④MODE key Use at the beginning/end to change or confirm set point/function.
⑤Main display Shows mainly chamber temperature and set temperature.
⑥Sub display Shows mainly time left and set time.
⑦Heater lamp Stays lit or blinks when heater is on.
⑧STERILIZE lamp Stays lit or blinks in sterilization process.
⑨DRY lamp Stays lit or blinks in drying process.
⑩END lamp Stays lit in the end of operation.
⑪STERILIZE lamp Blinks when “STERILIZE” menu is selected before operation, and
stays lit during operation.
⑫STERILIZE & DRY lamp Blinks when “STERILIZE & DRY” menu is selected before operation,
and stays lit during operation.
⑬TROUBLE lamp Stays lit when the unit is out of order.
⑭SET lamp Stays lit when pushing MODE key to check set point etc.,
①
②
④
③
⑫
⑪
⑭
⑬
⑩
⑨
⑧
⑤⑦⑥

12
Installation
Environmental Requirements
Area of installation
Never install sterilizer near any flammable,
explosive, or corrosive gas components.
Fire, explosion, or other troubles may occur
when turning the breaker on and off.
Area of installation
The sterilizer should not be installed in the
following environments.
①Under direct sun
②In temperatures below 5℃,or above 35℃
③Where severe changes in temperature occur
④In very humid or dusty locations
⑤Under water
⑥In vibrating or shock areas
Level surface
If the sterilizer is not on a level surface the
heater will reach a high temperature causing
a interruption of the operation.

13
Installation
Environmental Requirements
Provide ample space surrounding the sterilizer
Provide ample space surrounding the unit as
indicated below.
・Space for lid to open...13 inches or more
・Space to open door.....10.2 inches or more
・Space for ventilation...5.9 inches or more
Do not install under a low ceiling
An abundant amount of steam will spout out of the lid
just after operation. Do not install the sterilizer under
any electrical components such as an alarm.


Lock casters
Be sure to fasten the two front casters
after positioning the unit.
Release
Lock

14
Installation
Power Requirements
Ground
To prevent shock from electric current leak, be sure to connect the ground lead.
Contact Yamato Scientific or an electrician if a power source with a ground terminal is needed.
Never connect ground to with gas pipe, water pipe, telephone line, or lighting conductor.
SM200/300 SM310/510
Ground outlet is recommended for use. No power plug is attached to these types of units.
Ground properly according to power source.
Connect to sufficient power source
Type Power Source
Necessary
Capacity
SM200 AC115V
single-phase 13A or more
SM300 AC115V
single-phase 17A or more
SM310 AC220V
single-phase 11A or more
Be sure to connect the power cord to
sufficient power source.
(See right table)
SM510 AC220V
single-phase 11A or more
Attaching the power cord
Be sure that the breaker is turned off prior to connecting the power cord. The power cord plug is
not attached to the SM310 and 510. Select an adequate capacity plug and terminal according
to the connecting power source.
Green (Ground Lead)
Bl
ac
k
(
P
o
w
e
r
S
i
de)
Whi
te
(
P
o
w
e
r
S
i
de)
Power plug
G
r
ou
n
d
out
l
et
G
r
ou
n
d
l
ead

15
Installation
Initial Set Up
Filter Installation
Be sure to install the filter prior to operation.
Install the bottom plate
The bottom plate balances the material in the
chamber and protects heater 1 and sensor 2. Be
sure to install the plate.
Close the drain valve
Be sure to close the drain valve.
Bottom Plate
(at a level)
O
PEN
C
L
OS
E

16
Installation
Initial Set Up
Install the bottle
The sterilizer drains water forcibly during the “STERILIZE & DRY” process. Hot and high
pressure water will spout out to the bottle. To prevent the user from operating the sterilizer
without the drain bottle a micro switch will not allow the sterilizer to operate without the bottle.
①Open the front door of the sterilizer and
remove the bottle. The display will flash
indicating the bottle is not in place.
②Pour 1500ml of water into the bottle. This
water is used to cool the hot steam coming
out of the chamber.
The unit drains water forcibly out of the
container into the bottle during the
“STERILIZE & DRY” process. If too much
water is poured into the bottle, the drained
hot water can overflow. Follow the water
volume specified.
③Put the silencer into the bottle that contains
1500ml water.
④After the silencer is placed into the bottle,
push the bottle upward, inside the door
while
putting the exhaust hose into the bottle.
⑤Once the bottle is set, the micro switch is
pushed, which allows the sterilizer to
operate.

Silencer
Exhaust Hose
1500ml
Plug
Silencer
Exhaust Hose
Micro Switch

17
Installation
Initial Set Up
Pour water into the chamber
Pour water into the chamber according to the
gauge on the bottom plate.
Failing to pour adequate water will result in a
interruption of operation. Confirm the water
level after each sample run.
Requirement
SM200 1900〜2000ml
SM300 2800〜3000ml
SM310 2800〜3000ml
See table on right for water volume
information. Using over the maximum level
of water will result in hot water spouting
from the bottle.
SM510 2800〜3000ml
Use distilled water for sterilization
Use distilled water to prevent collection of mineral deposits and corrosion of chamber
components. Under ground water is not recommended.
Placing materials into sterilizer
Place the material into the basket and insert
into the chamber. Never block the steam
exhaust port. Always use a basket for
material being sterilized.
High-level
Exhaust Port
Low-level

18
Installation
Initial Set Up
Close the door before operation
Be sure to close the front door of the sterilizer before operation. An open door may cause the
bottle to fall during operation. Do not open the front door during operation.
Close the lid
Be sure to close the lid completely by turning
the handle clockwise in a 1/4-1/2 circle after
it touches the chamber. If it is not closed
properly, steam will escape from the
chamber.
Set the steam saucer
This unit comes equipped with a steam
saucer to prevent any steam from dropping
to the floor. See right illustration to place
the saucer under the exhaust.
CLOSE