YOKOGAWA CSU-X1 User manual

User’s Manual
Confocal Scanner Unit CSU-X1
IM 85A7C25-02E
IM 85A7C25-02E
横河電機株式会社 1ｓｔ Edition

-2-
TABLE OF CONTENT
1 Safety Precautions.....................................................................................5
1.1 Introduction ...................................................................................................5
1.2 CAUTION ......................................................................................................5
1.3 History ...........................................................................................................5
1.4 General Safety Precautions...........................................................................6
1.5 Laser safety Precautions ...............................................................................8
1.5.1 Laser Safety Standards ........................................................................................ 8
1.5.2 About the Class 3B Laser ................................................................................... 11
1.5.3 Safety Precautions .............................................................................................. 11
2 Product Overview ....................................................................................18
2.1 Principle of the Confocal Microscope........................................................... 18
2.2 Structure and Features of the CSU-X1.......................................................19
2.3 Description of CSU-X1.................................................................................20
2.3.1 CSU-X1-M1.......................................................................................................... 21
2.3.2 CSU-X1-M2.......................................................................................................... 22
2.3.3 CSU-X1-A1 .......................................................................................................... 23
2.3.4 CSU-X1-A2 .......................................................................................................... 24
2.3.5 CSU-X1-A3 .......................................................................................................... 25
3 Before Using the Unit..............................................................................26
3.1 Packing List .................................................................................................26
3.2 Option List ...................................................................................................27
3.2.1 Filter wheel control unit (CSU-X1CU-F1)......................................................... 27
3.2.2 Filter wheel (CSU-X1FW):.................................................................................. 27
3.2.3 Bright Field Control unit (CSU-X1CU-B1) ....................................................... 27
3.3 Installation................................................................................................... 28
3.3.1 Installation of Microscope................................................................................... 28
3.3.2 Installation of Laser System and Adjustment of Fiber Coupler...................... 31
3.3.3 Installation of CSU-X1........................................................................................ 32
3.3.4 Connection Diagram ........................................................................................... 37
3.3.5 Installation of Camera........................................................................................ 39
3.3.6 Power witch ......................................................................................................... 39

-3-
3.3.7 Power Off ............................................................................................................. 40
4 Name and Function .................................................................................41
4.1 M1 Model ..................................................................................................... 41
4.2 M2 Model ..................................................................................................... 44
4.3 A1 Model ......................................................................................................44
4.4 A2 Model ......................................................................................................45
4.5 A3 Model ......................................................................................................45
5 Maintenance ............................................................................................46
5.1 BA Filter Exchange .....................................................................................46
5.2 Dichroic Mirror Unit Exchange................................................................... 47
6 Trouble Shooting .....................................................................................48
6.1 Microscope, Laser and fiber coupler............................................................ 48
6.2 CSU-X1.........................................................................................................48
7 Specification.............................................................................................50
7.1 Product Specifications .................................................................................50
7.1.1 Specifications of CSU-X1 unit ............................................................................ 50
7.1.2 Electrical Specifications of CSU-X1 unit ........................................................... 53
7.1.3 Specifications of Control unit ............................................................................. 54
7.1.4 Specifications of Filter Wheel............................................................................. 55
7.1.5 Environment........................................................................................................ 56
7.1.6 Conformity........................................................................................................... 57
7.1.7 Global Environment Action................................................................................ 57
7.2 Model and Suffix Code (MS Code)...............................................................58
7.3 Dimension ....................................................................................................60
7.3.1 CSU-X1-A1 .......................................................................................................... 60
7.3.2 CSU-X1-A2 .......................................................................................................... 61
7.3.3 CSU-X1-A3 .......................................................................................................... 62
7.3.4 CSU-X1-M1.......................................................................................................... 63
7.3.5 CSU-X1-M2.......................................................................................................... 64
7.3.6 CSU-X1CU........................................................................................................... 65
7.3.7 CSU-X1FW .......................................................................................................... 65

-4-
7.4 Control Signal ..............................................................................................66
8 Command list...........................................................................................73
9 Warranty..................................................................................................75

-5-
1Safety Precautions
1.1 Introduction
Thank you so much for purchasing the CSU-X1. This manual covers the features,
operation methods and procedures, safety and handling precautions of the CSU-X1.
Before you start to use this instrument, read this manual thoroughly and operate the
instrument in a proper manner.
After reading through, please keep this manual in a safe place for future quick
reference.
1.2 CAUTION
No part of the user’s manuals may be transferred or reproduced without prior
written consent from Yokogawa.
Yokogawa continues to improve the product’s performance and features, and for this
reason, the specifications and information herein are subject to change at any time
and without notice.
The authors and publishers of this manual have used their best efforts in preparing
this document, but make no representation or warranties with respect to the
accuracy, applicability, fitness, or completeness of the contents of this manual. If you
have noticed any problems or have any suggestions for the manual, please contact
us.
To maintain long-term, stable performance of the product, please observe the
operation instructions in this manual.
1.3 History
1st Edition: September, 2007

-6-
1.4 General Safety Precautions
For the protection and safe use of this instrument, be sure to observe the instructions
on safety described in this manual when handling this instrument. If you use the
instrument in a manner not specified in this manual, the protection provided by this
instrument may be impaired. In addition, Yokogawa Electric Corporation assumes
no liability for the customer’s failure to comply with these requirements.
The following safety precaution symbols are attached to the instrument:
WARNING or CAUTION
Both of the above two symbols indicate that important content is mentioned. The
symbols are given wherever you are required to refer to the manual in order to avoid
personal injury and/or damage to the instrument.
The following safety precaution symbols are used in this manual.
WARNING
This symbol indicates that the operator must observe the explanation in the user’s
manual in order to avoid serious injury or death and/or damage to the instrument.
CAUTION
This symbol indicates that the operator must observe the explanation in the user’s
manual in order to avoid minor injury and/or damage to the instrument.

-7-
To reduce the risk of bodily injury of personnel such as electric shock or and/or
damage to the instrument, please comply the following precautions:
WARNING
Power supply
Please make sure the source voltage matches the voltage of the power supply before
turning on the power.
The CSU-X1 is classified as a laser hazard CLASS 3B instrument in accordance with
WHO regulations. Please comply with the remarks on laser safety precautions
described in the next chapter of this manual.
.
CAUTION
The CSU-X1 is used in combination with a microscope, laser system, camera, and
other peripheral instruments. Please read carefully instruction manuals of each
instrument, and follow safety precautions.
Please use input/output terminals of the CSU-X1 within the range of specification as
stated in this manual.
The CSU-X1 is a precision optical instrument. Please do not install the CSU-X1
system under inappropriate circumstances, such as vibrating conditions, dusty place,
very humid and/or high temperature room, drastic change in the room temperature,
corrosive or inflammable atmosphere, etc.
Do not touch inside the CSU-X1. Unauthorized open/touch inside the CSU-X1 may
cause mechanical and optical damages, and may cause serious damages.
Do not touch and soil the surface of the lenses inside the camera port adapter and C
mount port of the CSU-X1 at the time of installation or storing..
In the case of malfunction, please do not open the CSU-X1 but consult Yokogawa
Electric Corporation, or your local representative for proper advice and repair.
.

-8-
1.5 Laser safety Precautions
Laser products are classified on the basis of their laser beam wavelengths and power
characteristics, and a common set of safety standards is applied to the products in
each class. The CSU-X1 is classified as a laser hazard CLASS 3B instrument in
accordance with WHO regulations, which indicates that it is capable of producing
skin or eye damage if used improperly. Direct viewing into the laser beam or the
viewing of reflected beams must strictly be prevented. The WHO recommendations
concerning health and industrial protection when handling laser instruments must
strictly be observed. Before operating the CSU-X1, please read the following to
become familiar with its safety control, markings, and warnings.
1.5.1 Laser Safety Standards
The CSU-X1 is designed in compliance with the Emission Safety Guideline of Laser
Product of Japanese Industrial Standard (JIS C 6802). For safe use of the CSU-X1,
the following considerations are made:
Warning labels
You must attach the warning labels at appropriate positions indicated below either
on the CSU-X1 or on the microscope at installation, and strictly follow the warnings
when using the CSU-X1, for your own safety. Figure 1-1shows warning labels for
safe use of the laser product to be attached to this product, and the microscope to be
used in combination with it. Figure 1-2 shows the positions to attach the warning
labels for the CSU-X1 and the filter wheel. Figure 1-3 shows the positions to which
the warning labels for the microscope to be used in combination with this
instrument should be attached.
Warning label (1)
M3916LK(E)
Warning label (2)
M3916LL(E )

-9-
Warning label (3)
M3916LM(E)
Warning label (4)
D8953RH(E)
Warning label (5)
M3916MB
Warning label (6)
M3916XE
Figure1-1 Warning labels

-10-
(1) Warning label (1)
Warning sign to indicate this product belongs to the Class 3B laser product, and
indicates the product’s wavelength, maximum laser output power and laser class.
(2) Warning label (2)
Warning sign to instruct the operator must turn the laser off when he removes the
FC connector.
(3) Warning label(3)
Warning sign to indicate the laser aperture must be closed when the laser is on.
(4) Warning label (4) : For Direct view Port (Option)
Warning sign to instruct the operator avoid looking into the eyepiece of microscope
when exchanging the light path between confocal and non-confocal observation to
avoid laser exposure through the eyepiece.
(5) Warning label (5)
Warning sign to advise the necessity to use the laser in compliance with the class 3B
laser safety precautions. Specifically, it is necessary to avoid direct exposure of the
laser beam on the skin or eyes. Do not touch or look into the laser beam.
(6) Warning label (6)
Warning sign to indicate the operator must never insert the fingers or hand in the
filter wheel holes while operation.
(7) Laser key switch
The laser power supply used for CSU-X1 system employs a key switch for your safety.
The key switch is to turn off laser emission into the CSU-X1. For details, see the
user’s manual of the laser system you are using.
(8) Shutter
The shutter is for the incident laser beam. You may select full-open or close. For
non-confocal observation, always keep the shutter closed. Red LED light on the
operation panel of CSU-X1 will be OFF when the shutter is closed.
(9) Interlock
When you use the CSU-X1 without control unit (CSU-X1CU-F1), insert the interlock
key into the EXT CONTROL / INTERLOCK connector before turning on the unit. This
will disengage the interlock circuit, and you will be able to manually open/close the
shutter from the operation panel. If you use the control unit (CSU-X1CU-F1), insert
the interlock key into the EXT INTERFACE1 (at the back of the control unit). After
turn on the CSU-X1 unit, you will be able to open/close the shutter via operation panel
or control unit.

-11-
1.5.2 About the Class 3B Laser
Laser products are classified by their acceptable exposure limit ( AEL) based on the
laser beam wavelengths and power characteristics, and a common set of safety
standards is applied to the products in each class. The CSU-X1 is classified as a
laser hazard CLASS 3B instrument in accordance with WHO regulations, which
indicates that it is capable of producing skin or eye damage if used improperly.
Direct viewing into the laser beam or the viewing of reflected beams must strictly be
prevented. The WHO recommendations concerning health and industrial protection
when handling laser instruments must strictly be observed..
1.5.3 Safety Precautions
(1) Controlled access area
The laser products shall be operated within controlled access areas.
(2) Warning Signs
At the entrance of the controlled access areas, post laser warning signs.
(3) Surface reflection
Metallic and other lustrous and reflective surfaces may reflect laser beams. Make
sure that no unexpected reflection of laser beams may occur.
(4) Beam path
The laser beam coming through the optical fiber is injected into the CSU-X1 via an
FC connector. Never remove the FC connector connected to the CSU-X1 unless the
outgoing laser beam is stopped. Do not directly view or touch the laser beam source,
optical fiber, or laser beam coming out of the CSU-X1.
(5) Protective glasses
When you want to observe the direct light or reflected light, protect your eyes by
wearing protective glasses.
(6) Key control of laser sources
Power supply of laser system used for laser confocal scanner system can be turned
on/off by using a key provided with the laser system. When you don’t use the laser
system, please remove the laser key and store it in a safe place so that unauthorized
persons are not able to operate the laser. For details, refer to the user’s manual of
the laser system you are using in combination of the CSU-X1.

-12-
Figure 1-2 Positions to attach warning labels to CSU-X1-M1-E
(Labels are the same with the A1 model.)

-13-
Figure 1-3 Positions to attach warning labels to CSU-X1-M2-E

-14-
Figure 1-4 Positions to attach warning labels to CSU-X1-A2-E

-15-
Figure 1-5 Positions to attach warning labels to CSU-X1-A3-E

-16-
Figure 1-6 Positions to attach warning labels (6)
CSU-X1-M1
CSU-X1-A2
CSU-X1-A3
CSU-X1-FW

-17-
Figure 1-7 Examples of warning labels attached to the microscope

-18-
2Product Overview
2.1 Principle of the Confocal Microscope
As conventional optical microscopes use surface light sources such as halogen lamp
or others, diffused light from places other than the point of interest are mixed with
the light from the point of interest. Further, when planar photo detectors like
cameras or eyes are used, blurred, out-of-focus light from the points shifted toward
the optical axis are observed, overlapped with the observed light. For these reasons,
the conventional optical microscope has a limited spatial resolution. On the contrary,
confocal microscope systems use laser beams to illuminate samples with pin-point
accuracy, to eliminate diffused light from sources other than the point of interest. In
addition, pin-hole windows are provided in front of the light detector to cut off the
light from sources other than the point of interest for high resolution.
Figure 2-1 Principle of the confocal microscope
Detector Light source
Pinhole window
Objective lens
Focal plane
Specimen
Beam splitter