ABLE Cultisir User manual

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Cultistir
Magnetic stirrer for cell culture
Operating instructions

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Contents
I. Safety precautions................................................................................................3
II. Condition of use....................................................................................................4
III. Technical data ................................................................................................... 6
IV. Equipment provided..........................................................................................8
1. Controller and motor.........................................................................................8
2. Base plate ........................................................................................................10
V. Installation ......................................................................................................... 12
1. Assemble.......................................................................................................... 12
2. Installation space ............................................................................................ 12
3. Putting device into operation.......................................................................... 13
4. Disconnect the cables ...................................................................................... 13
VI. Operation......................................................................................................... 14
1. Starting (for 5mL) ........................................................................................... 14
2. Starting (for 30mL) ......................................................................................... 14
3. Starting (for 100mL) ....................................................................................... 15
4. Stopping........................................................................................................... 15
VII. Trouble shooting.............................................................................................. 16
VIII. Cleaning .......................................................................................................17
IX. Guarantee........................................................................................................ 18
X. Manufacturer and service contacts....................................................................18

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I. Safety precautions
General
Throughout the manual, the following symbol will be used to indicate general
precautionary measures:
Indication: information the correct use of Cultistir
Danger: Warning that, if safety precautions are not observed, injury to
personnel may result.
Warning: Warning that, if safety precautions are not observed, damage may
result to the instruments. Please read all documentation.
Installation: Location and conditions
Cultistir is intended for use in indoor laboratory.
Ambient conditions for Cultistir see chapter Technical data
Warning: Always locate Controller outside of incubator.
DO NOT leave unpowered Cultistir device in the incubator for more than a
month.
Avoid exposing Controller and power supply to direct sunlight or high
humidity.
Transport
Always grip Base plate of Cultistir when transporting.

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II. Condition of use
Applications
Cultistir is a spinning system comprising for stirring units for the gentle cultivation of
cells and the preparation of cell products in bioreactor. Cultistir is suitable for use with
cell suspension and cell aggregate formation (e.g. organoids or embryoid body of stem
cell) and immobilized adherent cells (e.g. micro carriers).
Product combinations
Cultistir is designed for using with ABLE single-use bioreactor of 5mL (Product No.
BWV-S005A), 30mL (Product No. BWV-S03A), 100mL (Product No. BWV-S10A).
Cultistir is not designed for using of any other culture vessel or bioreactor,
spinner flask.
Application not for the intended use
Cultistir is explicitly NOT intended for the applications:
Stirring of flammable or corrosive liquids
At danger atmosphere of explosion
Stirring of volatile liquid in a closed vessel
Dangers involved when safety precautions are not observed
Cultistir is safe to use. Some danger may exist if the instruments are used by untrained
personnel. Any person given the task of operating Cultistir must first read the
operating and safety instructions and understand them fully or be thoroughly trained
by a superior in the use of the instruments before operating the instrument.
Safety at work
Independent of any safety precautions listed here, all other relevant safety aspects (e.g.
regulations of each country or authority, rules of a company) must be observed.
Safety aspects for the user company
The supervisors of operating personnel must have read and understood all the safety
precautions involved and must ensure that these are adhered to. They must also ensure
that operating personnel also understand and observe all safety rules. Prior to

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operating Cultistir, the supervisor must ensure that no other dangers exist, especially if
other instruments and systems are also being used.
Changes in configuration/design by user
No changes may be made to Cultistir. Any alteration made releases ABLE corporation
from any liability for damage caused.
Power requirements
Checking the power supply
Check the power supply at the location and ensure that it complies with that of the
instrument.
Warning: Using the wrong power supply may damage electronic components
in the instrument.
Connecting to the power supply
Connect Cultistir to the power supply using the power cable provided.
Danger: The instrument may not be used if insufficient protection is provided
as this may expose personnel to the danger of electrical current injury.
NB: When connecting or disconnecting Motor from Controller the latter must
always first be switched off otherwise the electronics may be damaged.
Pollution degree
Pollution degree of this device is 2.

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III. Technical data
Item name
Controller and motor
Product No.
DW-1013
Controller
Dimensions (W × D × H)
132 × 150 × 41 mm
Weight (gross)
0.6kg
Power cable length
2m
Speed Range (Resolution)
5-120 rpm (1rpm)
Power source
AC100-240V±10%/50-60Hz/10VA
Operating condition
15-45°C/max., 80% RH
Altitude of the installation site
Use below 2000 m above sea level
Storage condition
-10-50°C/max., 95% RH
Motor
Dimensions (W × D × H)
58 × 90 × 84 mm
Weight (gross)
0.7kg
Motor cable length
2m
Speed Range
5-120 rpm
Power source
DC 12V
Operating condition
15-45°C/max., 99% RH/max., 10% CO2
Altitude of the installation site
Use below 2000 m above sea level
Storage condition
-10-50°C/max., 95% RH
Item name
6ch. Base plate for 5mL
Product No.
BWS-S005N0S-6A
Dimensions (W × D × H)
120 × 230 × 35 mm
Weight (gross)
1.0 kg
Speed Range
5-120 rpm
Stirring position
6
Applicable products
BWV-S005A
Power source
−
Operating condition
15-45°C/max., 99% RH/max., 10% CO2
Altitude of the installation site
Use below 2000 m above sea level
Storage condition
-10-50°C/max., 95% RH

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Item name
6ch. Base plate for 30/100mL
Product No.
BWS-S03N0S-6B
Dimensions (W × D × H)
153 × 267 × 33 mm
Weight (gross)
1.8kg
Speed Range
5-120 rpm
Stirring position
6
Applicable products
BWV-S03A, BWV-S10A
Power source
−
Operating condition
15-45°C/max., 99% RH/max., 10% CO2
Altitude of the installation site
Use below 2000 m above sea level
Storage condition
-10-50°C/max., 95% RH

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IV. Equipment provided
1. Controller and motor
Controller and motor (product No. DW-1013) comprises:
Controller
1
Motor
1
Power cable
1
Operating instructions
1
Power cable
Controller
Motor

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①Power switch: Switches the instrument on/off
②Stirring button: Switches stirring on/off
③▽/△: Up down keys change stirring speed values (rpm)
By keeping keys, values are displayed in rapid
④Display: Shows the speed set for stirring
⑤Rear port: Connects with motor cable of motor box
⑥Power cable port: Connects with power cable
⑦Motor case: Motor inside
⑧Base plate mounting port: Fixing Motor on base plate
⑨Motor cable: Connect with rear port of Controller
⑩Release ring: Use for disconnecting the motor cable connector
①
②
③④③
⑤
⑥
⑦
⑧
⑧
⑨
⑩

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2. Base plate
6ch. Base plate for 5mL (Product No. BWS-S005N0S-6A) comprises:
6ch. Base plate for 5mL
1
Fixing screw
2
①Reactor set position: 6 position of magnetic drive
②Motor mounting port: Fixing Motor on Base plate
③Fixing screw: Fixing Motor and Base plate
Base plate
②②
Fixing screw
①
③
③

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6ch. Base plate for 30/100mL (Product No. BWS-S03N0S-6B) comprises:
6ch. Base plate for 30/100mL
1
Fixing screw
2
Attachment ring
3
①Reactor set pin: 6 pins of magnetic drive
②Motor mounting port: Fixing Motor on Base plate
③Fixing screw: Fixing Motor and Base plate
④Attachment ring: Attachment adaptor for 100mL reactor
Base plate
Fixing screw
Attachment ring
①①
①①
①①
②②
③
④
③

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V. Installation
1. Assemble
Fix Motor and Base plate with fixing screws.
Controller and Motor is compatible with two types Base plate:
6ch. Base plate for 5mL (Product No. BWS-S005N0S-6A)
6ch. Base plate for 30/100mL (Product No. BWS-S03N0S-6B)
NB: When assembling Motor and Base plate or changing a device, must be
power off.
NB: Tighten the fixing screw by hand. DO NOT use tools.
NB: Always grip Base plate when transporting. DO NOT grip Motor.
2. Installation space
Base plate and Motor: W150 ×D300 ×H150 mm (inside the incubator)
Controller: W140×D150×H40 mm (It can be installed on the side panel)
The controller has a magnet sheet on the bottom of the case, so it can be
mounted onto the side panel of the incubator.

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3. Putting device into operation
①After cleaning of assembled Base plate and Motor, place into incubator.
②Put motor cable outside from the utility port of incubator.
③Connect power cable to the power cable port of Controller. Connect motor cable to
the rear port of controller. Align the notch of the port and motor cable connector and
push until it clicks. (DO NOT twist connector and cable)
④Connect the power cable to the power supply.
When operating the assembled motor and base plate side by side with the
controller on a table, install at least 30 cm apart.
NEVER use acetone, acid or alkali water for cleaning.
NB: In setting up Cultistir, please observe the conditions for location and
setting up as outlined in sectionⅠ.
4. Disconnect the cables
①Disconnect motor cable from rear port of the Controller. Pull the release ring of the
connector of motor cable. (DO NOT pull the cable)
②Disconnect the power cable.
NB: When disconnecting motor cable and power cable from Controller the
latter must always first be switched off otherwise the electronics may be
damaged.

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VI. Operation
1. Starting (for 5mL)
①Switch the power switch to ON position.
②Stirring speed is displayed on the display. Set the stirring speed with the △/up and
▽/down keys. During the setting, speed indicator on the display panel blinks.
③Place the bioreactor on base plate.
④Press the stirring (blue) button, a blip sounds, and the rotation starts.
⑤Stirring speed can be changed during operation.
2. Starting (for 30mL)
①Switch the power switch to ON position.
②Stirring speed is displayed on the display. Set the stirring speed with the △/up and
▽/down keys. During the setting, speed indicator on the display panel blinks.
③Place the bioreactor on set pin (6 position) of base plate.
④Press the stirring (blue) button, a blip sounds, and the rotation starts.
⑤Stirring speed can be changed during operation.
Display

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3. Starting (for 100mL)
①Place the attachment ring on small pins on Base pate surface (3 posion that are not
adjacent).
②Switch the power switch to ON position.
③Stirring speed is displayed on the display. Set the stirring speed with the △/up and
▽/down keys. During the setting, speed indicator on the display panel blinks.
④Place the 100mL bioreactor on set pins (3 position) of base plate.
⑤Press the stirring (blue) button, a blip sounds, and the rotation starts.
⑥Stirring speed can be changed during operation.
4. Stopping
①Press the stirring (blue) button, the rotation stops.
②If you do not operate for more than a month, Cultistir device must be taken out
from the incubator. Turn off the power to remove the device from the incubator.
DO NOT leave unpowered Cultistir device in the incubator for more than a
month.
Attachment ring

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VII. Trouble shooting
This product is maintenance-free. There are no periodic replacement parts. When any of
the following conditions appears, please contact our service.
①If the motor stops due to a failure, the controller issues a continuous beep sound.
②In spite of switched ON the stirring button, stirring does not start.
③The stirring speed of the controller is displayed but the impellor of the bioreactor
does not rotate.
④It is awkward rotation of the impellor of the bioreactor.
⑤Display does not turn on while the power switch is ON.

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VIII. Cleaning
Cleaning work
To clean the instruments, use a cloth moistened with customary cleansing agents like
soapy water, 70 % ethanol solution. DO NOT spray directly on the instrument but use a
cloth and wipe dry directly after cleaning.
Always switch off the instrument and disconnect it from power supply when
carrying out cleaning work.
NEVER use acetone, acid or alkali water for cleaning.

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IX. Guarantee
All instruments are guaranteed for 1 year.
X. Manufacturer and service contacts
Manufacturer:
ABLE Corporation
Tokyo, Japan
http://able-biott.co.jp/en/
Customer service:
Please contact your local distributor of ABLE products. To find out name and address of
your local distributor go to ABLE web site and inquire us.
http://able-biott.co.jp/en/contact/

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Document No:S-1475-DoC
Date of issue on : August 30, 2019
EU Declaration of Conformity
We ABLE Corporation 7-9, Nishigoken-cho, Shinjyuku-ku, Tokyo,
162-0812, Japan declares under our sole responsibility that the product described
below in compliance with the following directive
Hereby declares that the product described below:
Product Name: CULTISTIR（カルチスター）
Model Name: DW-1013, BWS-S005N0S-6A, BWS-S03N0S-6B
Complies with the provisions of the following EU Directives;
・EMC Directive 2014/30/EU
・Low Voltage Directive 2014/35/EU
・RoHS Directive 2011/65/EU
Based on the following harmonized standards applied.
・EMC Directive：EN 61326-1:2013
・Low Voltage Directive:EN61010-1:2010
・RoHS Directive：EN 50581：2012
・EN ISO 12100:2010
Done at Tokyo Japan,
Name：Haruo Ushiyama
Position：President Signature：
Address：7-9, Nishigoken-cho, Shinjyuku-ku, Tokyo, 162-0812, Japan
Technical Document File No.：S-1475-Index-Technical-File
ORIGINAL

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© 2019 ABLE Corporation
All rights to this documentation are reserved.