Accu-Scope EXI-410 Series User manual

MANUAL
73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F)
www.accu-scope.com • [e-mail]
EXI-410
INVERTED
MICROSCOPE SERIES

EXI-410 INVERTED MICROSCOPE SERIES
CONTENTS
SAFETY NOTES .................................................................................................................. 3
CARE AND MAINTENANCE ................................................................................................ 4
INTRODUCTION .................................................................................................................. 5
UNPACKING AND COMPONENTS ..................................................................................... 5
COMPONENT DIAGRAMS .................................................................................................. 6
MICROSCOPE DIMENSIONS............................................................................................ 12
ASSEMBLY DIAGRAM....................................................................................................... 13
DETAILED ASSEMBLY...................................................................................................... 14
OPERATION
POWERING ON THE MICROSCOPE.......................................................................................18
ILLUMINATION.........................................................................................................................18
INTERPUPILLARY DISTANCE.................................................................................................18
FOCUSING ...............................................................................................................................19
ADJUSTABLE TENSION CONTROL ........................................................................................20
STAGE PLATES (OPTIONAL) .................................................................................................20
SELECTING THE LIGHT PATH................................................................................................21
APERTURE DIAPHRAGM ADJUSTMENT ...............................................................................21
PHASE CONTRAST .................................................................................................................22
CENTERING THE LIGHT USING ANNULUS ...........................................................................22
EMBOSS CONTRAST ..............................................................................................................23
USING A MICROSCOPY CAMERA (OPTIONAL) ....................................................................24
USING FLUORESCENCE (OPTIONAL) ...................................................................................25
TROUBLESHOOTING........................................................................................................ 27
MAINTENANCE.................................................................................................................. 30
SERVICE............................................................................................................................ 30
WARRANTY ....................................................................................................................... 30

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 3
SAFETY NOTES
1. Open the shipping carton carefully to prevent any accessory, i.e. objectives or eyepieces, from
dropping and being damaged.
2. Do not discard the molded shipping carton; the container should be retained should the microscope
ever require reshipment.
3. Keep the instrument out of direct sunlight, high temperature or humidity, and dusty environments.
Ensure the microscope is located on a smooth, level and firm surface.
4. If any specimen solutions or other liquids splash onto the stage, objective or any other component,
immediately disconnect the power cord and wipe up the spillage. Otherwise, the instrument may be
damaged.
5. All electrical connectors (power cord) should be inserted into an electrical surge suppressor to
prevent damage due to voltage fluctuations.
6. Avoid blocking the natural air circulation for cooling. Ensure objects and obstructions are at least 10
centimeters from all sides of the microscope (the only exception is the table on which the microscope
sits).
7. For safety when replacing the LED lamp or fuse, be sure the main switch is off (“O”), remove the
power cord, and replace the LED bulb after the bulb and the lamp house has completely cooled.
8. Confirm that the input voltage indicated on your microscope corresponds to your line voltage. The
use of a different input voltage other than indicated will cause severe damage to the microscope.
9. When carrying this product, firmly grasp the microscope with one hand in the recess in the lower front
of the main body and the other hand in the recess in the rear of the main body. Refer to the figure
below.
Front recess
for grasping
Rear recess
for grasping

Do not grasp or hold using any other parts (such as the illumination pillar, focus knobs,
eyetubes or stage) when carrying the microscope. Doing so may result in dropping the
unit, damage to the microscope or failure of proper operation.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 4
CARE AND MAINTENANCE
1. Do not attempt to disassemble any component including eyepieces, objectives or focusing assembly.
2. Keep the instrument clean; remove dirt and debris regularly. Accumulated dirt on metal surfaces
should be cleaned with a damp cloth. More persistent dirt should be removed using a mild soap
solution. Do not use organic solvents for cleansing.
3. The outer surface of the optics should be inspected and cleaned periodically using an air stream from
an air bulb. If dirt remains on the optical surface, use a soft cloth or cotton swab dampened with a
lens cleaning solution (available at camera stores). All optical lenses should be swabbed using a
circular motion. A small amount of absorbent cotton wound on the end of a tapered stick such as
cotton swabs or Q-tips, makes a useful tool for cleaning recessed optical surfaces. Avoid using an
excessive amount of solvents as this may cause problems with optical coatings or cemented optics
or the flowing solvent may pick up grease making cleaning more difficult. Oil immersion objectives
should be cleaned immediately after use by removing the oil with lens tissue or a clean, soft cloth.
4. Store the instrument in a cool, dry environment. Cover the microscope with the dust cover when not
in use.
5. ACCU-SCOPE®microscopes are precision instruments which require periodic preventative
maintenance to maintain proper performance and to compensate for normal wear. An annual
schedule of preventative maintenance by qualified personnel is highly recommended. Your
authorized ACCU-SCOPE ® distributor can arrange for this service.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 5
INTRODUCTION
Congratulations on the purchase of your new ACCU-SCOPE ® microscope. ACCU-SCOPE ®
microscopes are engineered and manufactured to the highest quality standards. Your microscope will
last a lifetime if used and maintained properly. ACCU-SCOPE ® microscopes are carefully assembled,
inspected and tested by our staff of trained technicians in our New York facility. Careful quality control
procedures ensure each microscope is of the highest quality prior to shipment.
UNPACKING AND COMPONENTS
Your microscope arrived packed in a molded shipping carton. Do not discard the carton: the carton
should be retained for reshipment of your microscope if needed. Avoid placing the microscope in dusty
surroundings or in high temperature or humid areas as mold and mildew will form. Carefully remove the
microscope from the EPE foam container by its arm and base and place the microscope on a flat,
vibration-free surface. Check the components against the following standard configuration list:
1. Stand, which includes the supporting arm, focusing mechanism, nosepiece, mechanical stage
(optional), condenser with iris diaphragm, illumination system, and phase contrast accessories
(optional).
2. Binocular viewing head
3. Eyepieces as ordered
4. Objectives as ordered
5. Stage plate inserts, green and yellow filters (optional)
6. Dust cover
7. 3-prong electric power cord
8. Camera adapters (optional)
9. Fluorescence filter cubes (optional)
Optional accessories such as optional objectives and/or eyepieces, slides sets, etc., are not shipped as
part of the standard equipment. These items, if ordered, are shipped separately.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 6
COMPONENTS DIAGRAMS
EXI-410 (with Phase Contrast)
1. Phase Contrast Slider
2. Eyepiece
3. Eyetube
4. Viewing Head
5. Emboss Contrast Slider
6. Power Indicator
7. Illumination Selector
8. Main Frame
9. LED Lamp (transmitted)
10. Illumination Pillar
11. Condenser Set Screw
12. Field Iris Diaphragm
13. Condenser
14. Objective
15. Stage
16. Mechanical Stage with Universal Holder
(optional)
17. Mechanical Stage Control Knobs (XY
movement)
18. Focus Tension Adjustment Collar
19. Coarse Focus
20. Fine Focus
1
2
3
4
5
6
7
9
10
11
12
13
14
15
16
17
18
19
20
8

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 7
EXI-410 (with Phase Contrast)
1. Illumination Pillar
2. Field Iris Diaphragm
3. Phase Contrast Slider
4. Condenser
5. Mechanical Stage with Universal
Holder (optional)
6. Objective
7. Nosepiece
8. Power Switch
9. Eyepiece
10. Eyetube
11. Viewing Head
12. Light Path Selector
13. Camera Port
14. Power Indicator
15. Illumination Selector
16. Illumination Intensity Adjustment Knob
1
2
3
4
5
6
7
8
13
12
11
9
10
16
14
15

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 8
EXI-410 (with Phase Contrast)
1. Viewing Head
2. Stage
3. Emboss Contrast Slider
4. Main Frame
5. Focus Tension Adjustment Collar
6. Coarse Focus
7. Fine Focus
8. Condenser Set Screw
9. Phase Contrast Slider
10. Condenser
11. Illumination Pillar
12. Rear Hand Grasp
13. Mechanical Stage (optional)
14. Nosepiece
15. Fuse
16. Power Outlet
1
2
3
4
7
12
11
10
8
9
16
14
15
5
6
13

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 9
EXI-410-FL
1. Phase Contrast Slider
2. Eyepiece
3. Eyetube
4. Viewing Head
5. Fluorescence Light Shield
6. Emboss Contrast Slider
7. Power Indicator
8. Illumination Selector
9. Main Frame
10. LED Lamp (transmitted)
11. Illumination Pillar
12. Condenser Set Screw
13. Field Iris Diaphragm
14. Condenser Centering Screw
15. Condenser
16. Light Shield
17. Objective
18. Stage
19. Mechanical Stage with Universal Holder
(optional)
20. Fluorescence Illumination
21. Fluorescence Turret
22. Mechanical Stage Control Knobs (XY
movement)
23. Tension Adjustment Collar
24. Coarse Focus
25. Fine Focus
1
2
3
4
6
7
8
10
11
12
13
14
17
18
19
22
23
24
25
9
5
21
16
20
15

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 10
EXI-410-FL
1. Illumination Pillar
2. Field Iris Diaphragm
3. Phase Contrast Slider
4. Condenser
5. Mechanical Stage with Universal
Holder (optional)
6. Objective
7. Nosepiece
8. Fluorescence Turret
9. Fluorescence Turret Access Door
10. Power Switch
11. Eyepiece
12. Eyetube
13. Viewing Head
14. Light Path Selector (Eyepieces/Camera)
15. Camera Port
16. Power Indicator
17. Illumination Selector
18. Illumination Intensity Adjustment Knob
1
2
3
4
5
6
7
10
15
14
13
11
12
18
16
17
8
9

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 11
EXI-410-FL
1. Viewing Head
2. Fluorescence Light Shield
3. Emboss Contrast Slider
4. Main Frame
5. Focus Tension Adjustment Collar
6. Coarse Focus
7. Fine Focus
8. Condenser Set Screw
9. Phase Contrast Slider
10. Condenser
11. Illumination Pillar
12. Light Shield
13. Rear Hand Grasp
14. Mechanical Stage (optional)
15. Nosepiece
16. LED Fluorescence Light Source
17. Fuse
18. Power Outlet
1
2
3
4
7
13
11
10
8
9
18
15
17
5
6
14
12
16

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 12
MICROSCOPE DIMENSIONS
EXI-410 Phase Contrast and Brightfield
EXI-410-FL with Mechanical Stage

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 13
ASSEMBLY DIAGRAM
The diagram below shows how to assemble the various components. The numbers indicate the order of
assembly. Use the hex wrenches that are supplied with your microscope when required. Be sure to keep
these wrenches for changing out components or making adjustments.
When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 14
ASSEMBLY
Condenser
To install the condenser:
1. Unscrew the condenser set screw sufficiently to allow
the condenser tube to slide over the dovetail groove of
the condenser hanger.
2. Lightly press the condenser into position and tighten
the set screw.
Phase Contrast Slider
To install the phase contrast slider:
1. With the printed notations on the slider facing up and
readable from the front of the microscope, insert the
phase contrast slider horizontally into the condenser
slot. The orientation of the slider is correct if the edge
of the slider facing the operator has adjusting screws
visible.
2. Continue to insert the slider until an audible "click"
indicates that one position of the 3-postion phase
contrast slider is aligned with the optical axis. Insert
the slider further into the slot or backward to the
desired slider position.
Mechanical Stage (optional)
To install the optional mechanical stage:
1. Install the mechanical according to path (as shown
in the figure). First, align the edge A of the mechanical
stage with the edge of the flat/plain stage surface.
Align the mechanical stage with the plain stage until
the two set screws in the bottom of the mechanical
stage align with screw holes in the bottom of the plain
stage. Tighten the two set screws.
2. Install the universal holder according to path (as
shown in the figure). Start by placing the flat universal
holder plate on the plain stage surface. Align the two
screw holes on the universal holder plate with the set
screws on the lateral movement ruler of the mechanical
stage. Tighten the two set screws.
Set screw
Adjustment screws

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 15
Objectives
To install the objectives:
1. Turn the coarse adjustment knob ①until the revolving
nosepiece is at its lowest position.
2. Remove the nosepiece cap ② closest to you and
thread the lowest magnification objective onto the
nosepiece opening, then rotate the nosepiece
clockwise and thread the other objectives from low to
high magnification.
NOTE: ●Always rotate the nosepiece by using the
knurled nosepiece ring.
●Keep the covers on any unused
nosepiece openings to prevent dust and
dirt from getting inside.
Stage Plate
Insert the clear glass stage plate ①into the opening on
the stage. The clear glass allows you to view the
objective in position.
Eyepieces
Remove the eyetube plugs and fully insert the eyepieces
① into the eyepiece tubes ②.
①
②
①
①
②

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 16
Camera (optional)
To install the optional camera:
1. Remove dust cover from 1X relay lens.
2. Thread the camera into the relay lens as shown.
NOTE: ●Always keep one hand on the camera to
prevent it from falling.
3. Several camera relay lens magnifications are available
depending on application and/or camera sensor size.
a. A 1X lens is standard and included with the
microscope. This magnification is suitable for
cameras with sensor diagonal sizes of 2/3” and
larger.
b. A 0.7X lens (optional) will accommodate camera
sensors of ½” to 2/3”. Larger sensors may result
in images with significant vignetting.
c. A 0.5X lens (optional) accommodates ½” camera
sensors and smaller. Larger sensors may result
in images with significant vignetting.
Fluorescence Filter Cubes (EXI-410-FL models
only)
To install a fluorescence filter cube:
1. Remove the cover from the filter cube mounting port on
the left side of the microscope.
2. Rotate the filter turret to a position that accepts a filter
cube.
3. If replacing an existing filter cube, remove that filter
cube first from the position into which the new filter
cube will be placed.Align the filter cube with the guide
and groove before inserting. Insert completely until an
audible “click” is heard.
4. Replace the filter turret cover.
NOTE: ●Fluorescence filter sets must match the fluorescence LED excitation light source and
the fluorescence probes used in the application. Please contact with ACCU-SCOPE
with any questions about compatibility.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 17
Power Cord
VOLTAGE CHECK
Confirm that the input voltage indicated on the rear label of the microscope corresponds to your line
voltage. The use of a different input voltage than indicated will cause severe damage to your
microscope.
Connecting the Power Cord
Make sure the On/Off Switch is “O” (the off position) before connecting the power cord.
Insert the power plug into the power outlet of the microscope; make sure the connection is snug.
Plug the power cord into a power supply receptacle.
NOTE: Always use the power cord that came with your microscope. If your power cord becomes
damaged or lost, please call your authorized ACCU-SCOPE dealer for a replacement.

EXI-410 INVERTED MICROSCOPE SERIES
ACCU-SCOPE®73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com 18
OPERATION
Powering On
Plug the 3-prong line cord into the microscope power outlet
and then into a grounded 120V or 220V A.C. electrical
outlet. Usage of a surge suppressor outlet is highly
recommended. Turn the illuminator switch ①to “―”, then
press the illumination selector ② to toggle the light to on
(power indicator ③will light). For longer lamp life, always
turn the illuminator variable intensity knob ④to the lowest
illumination intensity setting possible before turning the
power on or off.
Adjusting the Illumination
The light level may need adjustment depending upon the
specimen density and objective magnification. Adjust the
light intensity for comfortable viewing by turning the light
intensity control knob ④clockwise (towards the operator)
to increase brightness. Turn counterclockwise (away from
the operator) to decrease brightness.
Adjusting Interpupillary Distance
To adjust the interpupillary distance, hold the left and right
eyetubes while observing a specimen. Rotate the
eyetubes around the central axis until the fields of view of
both eyetubes coincide completely. A complete circle
should be seen in the viewing field when viewing the
specimen slide. An improper adjustment will cause
operator fatigue and will disrupt the objective parfocality.
Where the “●” ①on the eyepiece tube lines up, that is the
number for your interpupillary distance. The range is 54-
75mm. Make note of your interpupillary number for future
operation.
①
②
④
④
③

EXI-410 INVERTED MICROSCOPE SERIES
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Operation (continued)
Adjusting the Focus
To ensure that you obtain sharp images with both eyes,
(since eyes vary, especially for those wearing glasses) any
eyesight variation can be corrected in the following
manner. Set both diopter collars ②to “0”. Using your left
eye only and the 10X objective, focus your specimen by
adjusting the coarse adjustment knob. When the image is
in view, refine the image to its sharpest focus by turning
the fine adjustment knob. Rotate the diopter collar to
obtain the sharpest focus. To obtain the same sharp
image using your right eye, do not touch the coarse or fine
adjustments. Instead, rotate the right diopter collar until
the sharpest image appears. Repeat several times to
check.
IMPORTANT: do not counter rotate the focusing knobs as
this will cause severe problems and damage to the
focusing system.
Focusing on a Specimen
To adjust focus, rotate the focus knobs on the right or left
sides of the microscope to move the objective up and
down. Coarse focus ①and fine focus ②knobs are
identified in the figure to the right.
The figure to the right illustrates the relationship between
the rotational direction of the focus knobs and the vertical
motion of the objective.
Focus travel: The default focus travel from the surface of
the plain stage is up 7mm and down 1.5mm. The limit can
be increased up to 18.5mm by adjusting the limit screw.
①
②
②
① ②
② ①