Accu-Scope EXI-600 User manual

MANUAL
73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F)
www.accu-scope.com • [e-mail]
EXI-600
RESEARCH GRADE
INVERTED BIOLOGICAL
MICROSCOPE SERIES

User Manual
This manual is written for EXI-600 research grade inverted biological microscope. To ensure the safety,
obtain optimum performance and to familiarize yourself fully with the microscope, it is strongly
recommended that you read this manual carefully before operating the microscope and put this manual in
a place easy to get for reference.
EXI-600 Research
Grade Inverted
Biological Microscope
User Manual

The warning and notice signal used in this manual
We provide you the most safety and reliable instruments, but improper operate and ignorance of
precautions may lead to a personal injury or a loss of property. To insure the right operating method,
we hope you read this manual carefully before you use the product. Besides, it’s highly recommended
you to put this manual in a place easy to get for easy reference.
In this manual the safety notices will be emphasized by the below symbols, please obey the
statement of these symbols.
The meaning of symbols on the product
Symbol Meaning
Conductor protect terminal
Caution of hot! Remind you of this situation:
The lamp house and spaces nearby will get hot when using. Do
not touch the lamp house in 30 minutes when it’s closed and when
it’s on. The lamp house may still extremely hot after it’s closed,
please ensure enough cooling time before replacing the bulb
This symbol appears in the electrical name board to remind you to
make sure the voltage of your area is the same to the input voltage
Turn on the power , rotate the brightness knob to adjust the
brightness of the field of view
Turn off the power
UV radiation
Turn off the power before open the device
Symbol Meaning
WARNING Ignore this symbol may lead to a serious personal injury even death.
NOTICE Ignore this symbol may lead to a personal injury or a property loss.

CONTENTS
Safety precautions ......................................................................................4
Ⅰ. Structure and names ........................................................................................................5
Ⅱ. Applications ....................................................................................................................6
Ⅲ. Assembly .....................................................................................................................7
Ⅳ. Operating and adjusting..............................................................................................15
Ⅴ. Basic observation ...........................................................................................................20
Ⅵ. Microscopy imaging .................................................................................................24
Ⅶ. Technical specifications .................. ...............................................................................26
Ⅷ. Configuration diagram ..................................................................28
Ⅸ. Trouble shooting ..................................................................................................29
Ⅹ.Maintenance ........................................................................................................31
Ⅺ. Product standard ..............................................................................................................31
Ⅻ. Company information .....................................................................................................31

Safety Precautions
1. Avoid placing the microscope in a place exposed to direct sunlight, with high
temperature, with high humidity or easily vibrated, make sure the platform is flat,
horizontal and stable enough. ( weight of the mainframe is 29.5kg)
2. When moving the microscope, hold the transport handle tightly and keep a distance
between the microscope and the platform before actually moving it.(shown in picture
above)
3. If bacterial solution or water is dripped over the stage, objective or observe tube, turn
off the power supply immediately and wipe out the solution, otherwise the instrument
maybe damaged.
4.
When operating, the lamp house can be considerably hot, make sure there is enough
space around the lamp house for heat dissipation to avoid the heat accumulates and
damage the instrument.
XD 5.
Before turn on the power of lamp house, make sure the power supply is connected properly.
Before replacing the bulb or fuse, turn off the power and wait until the lamp house cooled
Lamp House
Transport handle
Lamp House
Transport handle

completely.
★the indicate bulb: 12V 100WHAL high brightness halogen bulb (OSRAM)
★the fuse should load the correct melting current, do not use a temporary fuse in case of
the broken circuit.
6. Connect the power wires correctly, make sure the instrument is grounded in case of
lightning stroke.
7. Use the dedicated electric wires.
8.
The halogen lamp can radiate UV light thus may cause a burn of eyes and skins. Do
not see through the light directly and take protective measures when using the
microscope as much as possible.
9. The product should store in a place with shelter and no acid gas, alkali, organic solvent
or any harmful substances around.
★a 3-pin plug is reserved for the machine for safety concern , the machine is grounded
through the 3-pin plug. Do not use any other adapter plug or e the safety performance
may decrease.
★Do not put the instrument in a place where is hard to cut off the melting current.
★The protective offered by the device may be damaged if the device is not used in
the way indicated by our company.

Ⅰ
Structure and Names
Pic. 1

Component names:
1 Power switch
2 Left camera port
3 Left coarse/fine focusing handwheel
4 Side ports converter(light path convert—left and right side ports/observe)
5 Eyepiece
6 Trinocular tube
7 Brightness adjustment knob
8 Transmitted illumination shutter
9 Reflected illumination shutter
10 Right coarse/fine focusing handwheel
11 Middle magnification converter
12 Right camera port
13 X/Y axis handwheel of sample stage
14 Multifunction module turntable
15 Field diaphragm of reflected light
16 Aperture diaphragm of reflected light
17 3-hole filter spile
18 Obejective converter
19 Sample stage
20 Turntable condenser
21 Condenser centring screw
22 Lifting handwheel of condenser
23 Transmitted illumination support
24 12V 100W illumination lamp house
25 Filter support
26 Field diaphragm of transmitted light

Ⅱ
Applications
The EXI-600 inverted biological microscope is mainly used to study the cells, cultured
organization and sediment in culture bottles and culture dishes using transmitted and reflected
light.
This device can be used in bright field, dark field, phase contrast, differential
interference contrast, polarized light and fluorescence observation.
Main application of this device: study of human blood and tissue samples, observation
of the connection, activity and growth between live cells, drug reaction, minimally invasive,
external fertilization, toxicity test, digital record, automatic continuous time-point
observation, single molecule detection, etc.
Ⅲ
Assembly
Preparation before assembly and operation of the microscope
Tear down the packaging of the mainframe and the accessories.
The packaging include of mainframe, eyepieces, objectives, condenser support, lamp
house and other accessories such as filters, DIC blocks, dust-proof shield, tools and user
manual. The optional accessories will be packaged individually.
1. Tear down all the packaging, check the goods and confirm the accordance with the
products you purchased.
2. Dismantle the transport handles shown in pic.2.
Put the mainframe in a vibrate-proofed platform and then dismantle the handle with 4mm
hexagon screwdriver.
★Please take proper preservation of the handles.

④
Assembly
1. Trinocular tube
①Use a 2mm hexagon screwdriver to loosen
the bolt, take out the dust cover.
②Calibrate the trinocular tube and the swallow
tail, fix it by 2mm hexagon screwdriver.
★Do hold the trinocular tube tightly in case
of fallen down.
Pic.3
2. Eyepiece and centering telescope
①Take off the dust cover, insert the 2 eyepieces into the tube(the eyepiece should fully
contact with the tube).
★②When observing with phase contrast, replace one eyepiece with the centering telescope,
make the phase contrast ring focusing accurately.
★when using centering telescope
Pic.2
Transport handle
Mainframe
Bolt of trinocular tube
Trinocular tube
Swallow tail of trinocular tube

Pic.4
3. Graduated ocular
The added line scale may cause a slightly shift of
the image, rotate the visibility adjusting ring to
focus the line scale of the eyepiece.
Only need to screw off the line scale base to
Change the line scale.
4. Transmitted illumination support
Put the support at the back of
mainframe and tighten it with
M5X35 bolt and spring washer.
The transmitted support does not
need any adjustment.
Pic.6
Pic.5
Eyepiece
Dust cover
Centering telescope
Visibility adjusting ring
Zero visibility
Zero point
Line scale
Line scale base
Transmitted illumination support
Bolt
Spring washer
Pic.4

5. Objective
Screw off the dust cover on the converter, screw the
objective into the trapped hole
The objectives should match the lettering on the
converter by their magnification.
Screw the dust cover into other vacancies.
6. Sample platform
Pic. 7
Pic. 8
Pic. 9
Assemble the platform 135X85R/L
with the mainframe by three bolt
M4X10
★The platform can be assembled
at either right or left.(meanwhile
the X/Y hand wheel can be at
either right or left)
★The transmitted support can be
tilt a bit to the back to assemble the
platform.
Put the section bracket close to the spring on the edge of the platform and
press it to the platform levelly.
Dust cover Objective
X/Y hand wheel
Transmitted illumination support
Install bolt
Platform
Section bracket
Spring

7. Turntable condenser
Pic. 10
Align the guiding dowel of the condenser to the guiding groove of the support and
fix the condenser with the bolts after inserting it into the transmitted illumination
support.
Condenser centering bolt
Guiding dowel
Guiding groove
Condenser
Fixing bolt
Illumination support

8. Microscope power port
Pic.11
Please off the power before installing any parts and accessories.
★The TL lamp house switch can control the transmitted lamp house without
turning off the power.
★
The RL lamp house switch can control the reflected lamp house without
turning off the power.
Transmitted port
Reflected port
Transmitted/reflected switch
Indicator light
Power port
Power switch
Brightness adjust knob
Power light
Reflected illumination switch
Lamp house switch

9. Illumination lamp house (halogen)
Pic. 12
Insert the swallow tail of the lamp
house to the socket, make the lamp
house parallel to the stable table
and then fixed it with bolts
Connect power wires by the port
position shown in pic.11.
Check the connection and open
the power supply, rotate the
potentiometer knob and see if
the brightness changes.
Swallow socket
Lamp house
Fixing bolt

Pic.13
Assemble the diffuser
Locate the two round grooves of the
diffuser to the two bulged on the
swallow tail socket and screw the
diffuser into the socket groove.
Screw it reversely when taking the
diffuser out.

Ⅳ
Operating and adjusting
1. Coarse and fine focusing hand wheel
move range：10mm
coarse focusing hand wheel：2mm/cycle
fine focusing hand wheel：0.2mm/cycle
★Focusing hand wheel is set at both side
Of the mainframe
The left hand wheel has scales
The right hand wheel has no scales
2. Side port converter/light path converter—observe from left, right port and ocular
The side port converter has three instruction of different splitting ratio.
Pic.14
Pic. 15
★：
vis---observe by ocular
camera---photonic
Fine focusing hand wheel (scale)
100%vis: 0% camera
20%vis: 80% camera left
0%vis: 100% camera right
L
eft port screw
thread
Splitting tag
Right port screw
thread

3. Turntable condenser
(transmitted light)
NA:0.55
WD：26mm
Condenser turntable/6 positions
Bright field：H
Phase contrast：PH1, PH2, PH3
Ⅱ
Adjust the aperture diaphragm: rotate the diaphragm plate
6 position adjust: rotate the condenser tunable, if you turned the bright field/H into the light
path, make the mark “H” face you.
Phase contrast ring centering bolt: use 1.5mm hexagon screwdriver to adjust the phase
contrast ring to match the objective.
Replacing DIC prism
Take out the condenser and put it on the plate form
before replacing the DIC prism
Take out the dust cover, screw out the pressure washer
with matched tools, and turn over the condenser to
let the DIC prism slip off.
Put the new DIC prism to the groove of condenser,
screw the pressure washer in and cover the dust cover,
shown in pic.17
★attention the direction of the DIC prism and clip it
into condenser carefully to protect the prism
surfaces.
★make sure the scale ring in the tunable is right.
Pic. 16
Pic. 17
Centering bolt
Aperture diaphragm plate
Condenser turntable
Dust cover
Pressure washer
Pressure washer

4. Transmitted light field diaphragm
5. Middle magnification converter
The middle magnification mark: 1X/1.5X
You can directly convert the middle magnification the change the observe magnification.
When using transmitted illumination, screw
the tunable to adjust the field diaphragm.
Screw the condenser lift hand wheel to lift
the condenser and set the observe height.
The condenser is fixed by fixing bolts.
Screw the 2 condenser centering bolts to
move the condenser to the center of the
light path.
The filter bracket can hold different filters.
Pic. 18
Pic. 19
Field diaphragm tunable
Condenser
centering bolt
Filter bracket
Fixing bolt
C
ondenser lifting
hand wheel
Magnification mark
Magnification turntable