Accuris MR9610 User manual

Operation Manual
Version 2.0
MR9610,
SmartReader™UV-Vis
Microplate Reader
This instrument is for research use only

III
Foreword
Thank you for purchasing our MR9610, SmartReader™UV-Vis Microplate
Reader. This user manual describes how the instrument works and includes an
operation guide, please read it carefully before operation and keep for future
reference.
Initial check
Please check the instruments as well as all accessories with the packing list when
first received and unpackaged. If you find any missing, please contact your
distributor or Accuris Instruments.
Safety Warning and Guidelines
1. Important information for safe use
Please read the manual carefully and operate safely according to all
guidelines.
This instrument is intended for Research Use Only
Users should have a clear understanding on use this instrument before
operation. Please read this user manual carefully.

IV
2. Safety Tips
The operation, maintenance and repair of the Instrument should comply with the
basic guidelines and the warnings below.
Indoor use only.
Warning: Biological contamination hazard. All samples for testing,
quality control, and calibration should be regarded as infectious, and
any parts that come into contact with samples should also be treated
as infectious. Please wear gloves and appropriate safety gear when
operating this device.
Before using the device, read the operation manual carefully. These
instruments are designed for use in a laboratory environment. The
device must be operated by skilled laboratory personnel with the
appropriate training.
Warning: Avoid injury. Keep your body or any part of body at a safe
distance (1 foot or more) from the instrument when running.
The operator should not open or perform any repairs on the
Instrument without specific instructions from Accuris Instruments or
an authorized agent of Accuris. Performing any unauthorized
service repairs will result in loss of warranty coverage. Contact
Accuris Instruments or an authorized distributor or agent in the event
of any service issues.
Before powering on, confirm that the voltage used meets the
electrical requirements of the instrument as stated on the rating plate.
If the electric cord is damaged, replace it with the same type.
Hold the socket firmly when you pull the plug from an outlet, and don’t
pull the electric line only.

V
The Instrument should be installed in an environment of standard
room temperature, low dust, low humidity and away from direct
sunlight. Also make sure there is adequate ventilation, no corrosive
gas or strong magnetic field.
Always power off the instrument when you are finished using it.
Unplug the power cord and cover the instrument with a cloth or plastic
sheet to prevent excessing dust from entering the housing.
Pull the connector plug from the electrical outlet immediately and
contact the vendor in the case of:
⚫Liquid entering the housing.
⚫Abnormal operation: such as any abnormal sound or smell.
⚫The instrument is dropped or there is any damage to the housing.
⚫Any malfunction

Contents
Foreword................................................................................................................................................III
Initial check...........................................................................................................................................III
Safety Warning and Guidelines..............................................................................................................III
1. Important information for safe use....................................................................................III
2. Safety Tips............................................................................................................................IV
Chapter 1 Introduction....................................................................................................................1
Chapter 2 Features ................................................................................................................................2
Chapter 3 Instrument Overview....................................................................................................4
Chapter 4 Installation............................................................................................................................5
1. Initial check................................................................................................................................5
2. Installation location and environment.....................................................................................5
3. Installation steps .......................................................................................................................5
Chapter 5 Operation Guide......................................................................................................................7
1. Initial self-check.........................................................................................................................7
2. System settings .....................................................................................................................8
3. Protocol Management...........................................................................................................9
4. Reading a Microplate..........................................................................................................12
5. Result processing................................................................................................................18
6. Report exporting..................................................................................................................26
7. Power off...............................................................................................................................27
Chapter 6 Maintenance, storage, transportation.........................................................................28
1. Maintenance.........................................................................................................................28
2. Storage and transportation ................................................................................................28
Chapter 7 Trouble shooting.................................................................................................................29
Chapter 8 Accessories............................................................................................................................31

MR9610 Microplate Reader Operation Manual Chapter 1 Brief Introduction
─1─
Chapter 1 Introduction
The SmartReader™UV-Vis is an automated microplate reader for ELISA
assays. Through absorbance readings, it can determine the concentration, as
well as positive or negative detection of antibody and antigen in samples when
used with ELISA kits and solutions. The instrument can also be used to test for
purity and quantitate nucleic acids and proteins.
Key Features:
1) 10-inch color touch screen.
2) Operating system allows acquisition, editing and saving of data.
3) Stand-alone system incorporates built-in processing, it can be optionally
connected to a PC with the use of SmartReader software.
4) Graphical interface displays a representative plate for easy setting of well
parameters: blank, sample, standards, positive/negative, quality control
and multi-value comparisons.
5) Dual optical channels, one used for sample detection, and one used for
reference.
6) End-point method, kinetic readings, and spectral scanning can be
performed.
7) Accepts plates with or without lids. Top heating prevents condensation.
8) Xenon lamp offers long life: 109flashes (1,000,000,000), equivalent to
approximately 1,300,000 plate reads.
9) Plate incubation feature allows accurate and uniform temperature control
(≤0.5°C deviation between wells).
10) Top reading optical path with continual self-checking and automatic
calibration option for monochromator wavelength and plate positioning.
11) Shaking function, time and speed are adjustable.
12) USB flash drive data export, for fast and easy data transfer.
13) Multi-user operation, data management for separate users.

MR9610 Microplate Reader Operation Manual Chapter 2 Features
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Chapter 2 Features
Working conditions：
Ambient temperature: 10C~40C
Relative humidity: 30%~80% (Non-condensing environment)
Power: AC 100-240V 50-60Hz 2A
Parameters:
Model
Parameter
MR9610
Light source
Xenon flash lamp >109flashes
Wavelength
200～1000nm
Bandwidth
< 2.5nm
Wavelength accuracy
±2nm
Wavelength
repeatability
0.2nm
Read-out range
0.0-4.0 OD
Linearity@450nm
R2≥0.999, [0.0 –3.0]
Accuracy@450nm
1.0% + 0.003 Abs (0 - 2.0]
2.0% (2.0 - 2.5 Abs]
Precision@450nm
CV < 0.5% or SD<0.003 (Precision mode); CV < 1.0%
(Fast mode)
Stability@450nm
< 0.005A , (0.0 - 2.0]
< 2% , (2.0 - 2.5]
Measurement speed
< 8 seconds in Fast mode (96-well plate)
< 28 seconds in Precision mode (96-well plate)
< 74 seconds in Precision mode (384-well plate)
Plate shaking
Linear (Slow: 16.8Hz, 1mm; Medium: 6.68Hz, 3mm;
High: 2.44Hz, 15mm)
Incubation range
RT+ 5°C to 45°C
Temp. uniformity
± 0.5°C @ 37°C

MR9610 Microplate Reader Operation Manual Chapter 2 Features
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Connections
1 B-type USB port for PC connection
1 Ethernet port
2 A-type of USB ports for mouse and printer connections
Power requirements
Included power adapter: DC24V 6.67A 160W,
AC 100V –240V
Dimension (W×D×H)
300×500×260mm / 11.8 x 19.7 x 10.25 inch
Weight (kg)
15.5kg / 34 Lbs

MR9610 Microplate Reader Operation Manual Chapter 3 Instrument Structure
─4─
Chapter 3 Instrument Overview
Structure
Rear cover
Touch screen
Housing
External Buttons
Plate holder
Front door
Extension module
USB Ports
LAN Port
Power Switch
DC Power Inlet
B-type
USB Port
Air outlet

MR9610 Microplate Reader Operation Manual Chapter 4 Installation
─5─
Chapter 4 Installation
1. Initial check
Each MR9610 is thoroughly tested before shipping, but please check it again when you
receive the instrument and contact your local distributor or Accuris Instruments if:
• The outer package is damaged
• The outer package has any obvious moisture stains
•The outer package has marks of impact
After opening, please check the instrument and box contents.
Confirm that all ordered accessories have been included.
Check the instrument’s appearance for any damage.
2. Installation location and environment
Installation location: locate the instrument on a flat, dry, clean and stable surface, allow
enough room on the front side so there is space for plate holder drawer to move in and out,
and also keep 15cm / 6 inches of space behind and on the sides for any wires and air flow.
Environment:
a. The environment for installation should not be excessively humid or dusty.
b. Temperature should be within the range of +10°C ~ +40°C.
c. Relative humidity should be within the range of 30% ~ 80% to avoid condensation.
Note: KEEP THE INSTRUMENT AWAY FROM CORROSIVE GASSES AND
LIQUIDS.
3. Installation steps
①Place the instrument on a stable and level surface.
Note: DO NOT loosen any screws or parts without first consulting with the
Accuris Instruments Service Department or your authorized distributor. Doing
so can cause damage and void the warranty.
②Connect the instrument to an appropriate power outlet using the provided power
cord.
Fig. 1

MR9610 Microplate Reader Operation Manual Chapter 4 Installation
─6─
Note: One cord coming from the power adapter will fit to the input jack on the
back of the instrument. Make sure to connect it according to the above picture.
The other cord will connect to a standard electrical outlet (100VAC to 230VAC)
③After connecting the power cords, switch the power button on the rear of the
instrument to the ON position, the front panel will cycle through a start-up and
self-test screen.
Warning: Do not connect the instrument to a power outlet that is not properly
grounded.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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Chapter 5 Operation Guide
1. Initial self-check
This chapter explains a standard protocol set up and operation, beginning with the
automatic self-check after power on. The following screen image will appear during the
self-check:
Fig 2
The user login interface will appear after the self-check, see Fig 3. This allows login
as the Admin, existing user, or click “Guest”.
Fig 3

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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Table 1
User type
Creation
Method
Default password
Permission
Export
Admin
Cannot be
deleted
“123456”
For all files of
Admin, User and
Guest
All can be
exported
User
Created by
Admin
Default is “123456” or
is set when creating
Access to files
under the user
account
Guest
Cannot be
deleted
No password
Note: Please keep the Admin password recorded and in a safe place, and contact
Accuris Instruments or your distributor if the Admin password is lost or forgotten.
After logging in, the Home Interface screen is displayed (Fig 4.)
Fig 4
The “Admin” button on the lower left is for the logout and login interface.
2. System settings
In the “Systems” section, users create system settings (time, date and language)
according to their needs, for details see Fig 5.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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Fig 5
Note: 1. The instrument must be manually powered off and back on after the date and
time settings are changed.
2. The Maintenance function is accessible only by authorized service
representatives.
3. Click the “Home” button on the top left corner to go to the main interface.
3. Protocol Management
Click “Absorbance” on the home interface to enter the layout interface (fig 6). This
interface is composed of six sections: the navigation bar at the top, the sidebar, the main
display area, the optional bar, setting type selection and the status bar at the lower right
area. The SmartReader UV-Vis incorporates a touch screen control panel, but the
included mouse can also be used for all programming functions.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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─
Fig 6
Protocol name can be modified by clicking it directly.
The default name of a new protocol is the system date
and time, and it can be modified manually. Apop-up
window will appear to remind the user when a modified
protocol has not been saved.
Save the current protocol to the protocol list.
Shortcut for exporting raw data to a flash drive in .csv
format. (Exports only the data displayed in the “Main
Display Area”)
Open the Protocol folder for accessing saved protocols,
sorting, deleting, importing, exporting, and renaming
protocols. The Protocol list opens on the right side of the
screen and can be closed by clicking on the left area.
Navigation bar
The optional bar
Setting Type Selection
The Sidebar
Main display area

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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─
Protocol list, standard state Protocol list in “edit” state
Fig 7
When the protocol list is in the standard state, users can perform the below operations:
⚫Search: Enter a keyword to search the list.
⚫Sort: protocols can be sorted according to “Name”, “Date” and “State”. “demo1”,
“demo2” and “demo3” are always displayed at the first three positions.
⚫Import: For importing protocols from a flash drive to the instrument.
⚫Rename: Change the name of a protocol.
⚫Save as: Save as a new protocol.
⚫Edit: “Edit” button is located on the top right corner, see Fig 7 to enter the edit state.
⚫Account: Protocols of other accounts can be checked, but this function is only
available for the Admin account.
When the protocol list in the edit state, the below operations are available:
⚫Sorting: protocols can be sorted according to “Name”, “Date” and “State”.
⚫Checkbox: “” can used to select more than one for batch operation.
⚫Delete: delete selected protocols.
⚫Export: For exporting selected protocols to a flash drive.
⚫Cancel: Return to the standard state of the protocol list.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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4. Reading a Microplate
After a protocol name is created, the next step is to set the parameters according to
the experiment requirements.
4.1 Plate layout settings
The interface will automatically display the plate in a 96 well plate layout when
creating a new protocol. To change from a 96 to 384 well-layout, click on the top left of the
plate layout to choose the required layout format.
Fig 8
The plate layout menu allows choosing 6 well-setting options, as well as a clear option.
Choose the appropriate setting type, and the option bar will change accordingly, then click
the corresponding wells on the main display area to finish the settings.
Note: the entire plate (all wells) can be selected by clicking the blank box at the
upper left corner of the plate layout.
Blank: Set for a blank control during measurement. The
average absorbance value of the blank wells is
subtracted from the absorbance values of the other wells
after a plate read.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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─
Standard: Used for creating the standard curve. After
clicking “Standard”, the option bar will allow setting
replicates and also calculations for quickly setting the
standard concentrations, see Fig 9.
⚫Replicates: Use to set the same standard for multiple
wells.
⚫Concentrations: After setting the concentration and
unit of the first well, the protocol will calculate and set
subsequent standards automatically according to the
set operator and step size, or the values can be
modified manually by clicking the corresponding
wells.
Example: If the set concentration is 125ng/μL, the
operator is “×” (multiply) and the step size is “2”, the
concentration of the first well will be 125ng/μL, the
second well 250ng/μL (2 x 125ng/μl) and the third
500ng/μL (2 x 250ng/μL)… etc.
Quality control. Settings are done in the same manner as
the wells of standard samples.
Unknown samples. Users can set wells as unknown, and
there in an additional “Factor” option if the unknown
sample has been diluted. If a dilution has been used,
set the value for the dilution factor “X. 1:X means the
unknown sample was diluted X times.
Positive Control, users can set several wells as positive
controls
Negative Control, users can set several wells as negative
controls
Used for clearing the settings of the plate layout.

MR9610 Microplate Reader Operation Manual Chapter 5 Operation Guide
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Fig 9
Note: The plate layout can be set up before or after the plate is read. For
example, users can set all wells as “unknown”, read the absorbance values, then set
up the layout of the plate. The absorbance value data and layout are separate,
users can create or modify a layout to analyze absorbance data that was captured in
the past.
Note: the absorbance values of a test cannot be changed!
4.2 Parameter setting
After completing the plate layout settings, click “Run” on the left side bar (see Fig 10.)
Fig 10
After clicking “Run” the lower window becomes a controller with the following functions: