Adrona Crystal EX Trace User manual

Water purification system
Adrona Crystal EX Trace/
HPLC/ Bio
INSTALLATION & OPERATION MANUAL
Version 5.1 (2019)
No. LVRIG38118A

INTRODUCTION
2
Table of Contents
INTRODUCTION ........................................................................................................................................ 3
Using This Manual................................................................................................................................ 3
Safety Information ............................................................................................................................... 3
Contact Adrona .................................................................................................................................... 3
PRODUCT OVERVIEW ............................................................................................................................... 4
Crystal Ex Water System General Description ..................................................................................... 4
System Overview ................................................................................................................................. 4
Water Specifications ............................................................................................................................ 5
Technical Specifications ....................................................................................................................... 5
System Components in Flow Chart ...................................................................................................... 6
Principle ............................................................................................................................................... 7
PRE-INSTALLATION ................................................................................................................................... 8
Feedwater requirements ..................................................................................................................... 8
Feedwater connection ......................................................................................................................... 8
Site requirements ................................................................................................................................ 9
Environmental requirements ............................................................................................................... 9
Water leakage safety ........................................................................................................................... 9
Additional equipment .......................................................................................................................... 9
UNPACKING .............................................................................................................................................. 9
INSTALLATION ........................................................................................................................................ 10
Preparation of the System ................................................................................................................. 10
Introduction to John Guest tubing system ........................................................................................ 10
Rear and side panel ........................................................................................................................... 11
Pro tank.............................................................................................................................................. 12
Installation of filter ............................................................................................................................ 13
Installation of the modules ................................................................................................................ 14
Dispense filter mounting ................................................................................................................... 16
Connection of tubing ......................................................................................................................... 18
Connection to tank cable ................................................................................................................... 20
Calibration ......................................................................................................................................... 21
USING THE SYSTEM ................................................................................................................................ 22
Display ............................................................................................................................................... 22
Obtaining the water ........................................................................................................................... 23
Operating modes ............................................................................................................................... 25
MAINTENANCE ....................................................................................................................................... 27
Maintenance schedule ...................................................................................................................... 27
Replacement of pre-filter .................................................................................................................. 28
Replacement of deionization or polishing module ............................................................................ 29
Calibration ......................................................................................................................................... 31
Empty the tank .................................................................................................................................. 32
Cleaning and Sanitization of the system ............................................................................................ 33
TROUBLESHOOTING ............................................................................................................................... 34
SPARE PARTS AND CONSUMABLES ........................................................................................................ 35
WARRANTY AND CLAIMS ........................................................................................................................ 35

INTRODUCTION
3
INTRODUCTION
Using This Manual
This is a user manual for Adrona Crystal EX Trace, Crystal EX HPLC and Crystal EX Bio water purification
systems. We strongly advise you to read this manual before installing and using the water purification
system.
Safety Information
WARNING! Read and understand all sections in this guide before installing or operating the system.
The symbols used below are internationally accepted symbols that warn of potential hazards with
electrical products.
This HAZARD symbol is used to refer to instructions in this manual that need to
be done safely and carefully
This ATTENTION symbol is used to refer to instructions in this manual that need
to be done carefully
This ELECTRICAL GROUND symbol is used to refer to a position where an
electrical ground connection is made
This ELECTRICAL DANGER symbol means that there are dangerous voltages
present within the unit
This DANGER symbol indicates that it is necessary for the user to refer to the
owner’s manual, read, understand and follow the instructions
This UV RADIATION symbol indicates the ultraviolet radiation (UV) danger.
Failure to comply with safety instructions may result in personal injury
Ensure that anyone who operates the water purification system has received instructions in both
general safety practices for laboratories and specific safety practices for the instrument.
Contact Adrona
Adrona SIA
Kalnciema st. 209
Riga, LV-1046
LATVIA
Phone: [phone]
Mob: [phone]
[e-mail]
www.adrona.lv

PRODUCT OVERVIEW
4
PRODUCT OVERVIEW
Crystal Ex Water System General Description
Water purification system Crystal EX Trace/ HPLC/ Bio produces pure water that complies with ISO 3696
Grade I and Grade II water requirements.
Pure (ISO 3696 Grade II) water applications include, but are not limited to:
 Feed for laboratory equipment (washing machines, clinical analyzers,
humidifiers, autoclaves, hydrogen gas generators);
 Manufacturing of chemical and biochemical reagents;
 Microbiological media preparation;
 Spectrophotometry.
 Buffer preparation;
 Wet chemistry;
 In some cases - sensitive analytical techniques (e. g. atomic absorption, ICP-OES).
Ultrapure (ISO 3696 Grade I) water applications include but are not limited to:
 High sensitivity analytical techniques (e. g. ICP-MS).;
 High performance liquid chromatography;
 Molecular biology;
 Cell culture.
“Crystal” system combines several water purification technologies (depending on configuration):
reverse osmosis, adsorption, deionization (DI), UV-sterilization, photooxidation.
The water storage tank is used for storing Grade II water.
System Overview

PRODUCT OVERVIEW
5
Water Specifications
Purified water specification Crystal EX Trace Crystal EX HPLC Crystal EX Bio
Grade II resistivity at 25 °C >10 M x cm >10 M x cm >10 M x cm
Grade II conductivity at 25 °C <0.1 µS/cm <0.1 µS/cm <0.1 µS/cm
Grade I resistivity at 25 °C 18.2 M x cm 18.2 M x cm 18.2 M x cm
Grade I conductivity at 25 °C 0.055 µS/cm 0.055 µS/cm 0.055 µS/cm
TOC level <10 ppb <2 ppb <2 ppb
RNase N/A N/A <0.01 ng/mL
DNase N/A N/A <4 pg/

L
Bacteria <0.01 CFU/mL <0.01 CFU/mL <0.01 CFU/mL
Endotoxins <0.15 EU/mL <0.15 EU/mL <0.001 EU/mL
Technical Specifications
Dimensions and weight
Crystal EX Trace Crystal EX HPLC Crystal EX Bio
Dimensions (W*D*H) 40*35*55 cm 40*35*55 cm 40*35*55 cm
System weight 17 kg 18 kg 19 kg
Operating weight 24 kg 25 kg 26 kg
Noise level
Water purification system Crystal EX can generate a maximum sound pressure level of 47 dB at 1 m
distance from the system.
Electrical requirements
The water purification system is configured for 230 V ± 5%, 600 mA max.
In areas where the supplied power is subject to voltage fluctuations exceeding 10% of the nominal
volume, a power line regulator may be required.
Power supply receptacle should be within 1.5 meters from the installation site.

PRODUCT OVERVIEW
6
System Components in Flow Chart
Crystal EX Trace
Crystal EX HPLC
Crystal EX Bio

PRODUCT OVERVIEW
7
Principle
The hydraulic diagram of the Crystal water purification system is shown above.
The solenoid valve controls intake of feed water from the tap. The first purification stage consists of
pre-filter (part no. 10320) and activated carbon filter intended to remove particles, free chlorine,
organics and colloids. A pressure switch controls feed water pressure.
The boost pump is used to maintain pressure at the level necessary for the efficient operation of the
reverse osmosis membrane. The feed stream flows to the membrane where it splits into two parts: the
permeate, diffused through the membrane, and the concentrate which passes over the membrane,
carrying away contaminants to drain. The permeate flows to the tank EX RO or to the third purification
step – deionization module (part no. 10310) where the remaining ions are removed.
Before entering the tank, water is sterilized by optional UV lamp (in Bio configuration, part no. 10102);
water quality is controlled by water conductivity sensor. The LCD display provides information about
the system status.
Purified water is stored in the tank. Water in the tank meets the requirements of ISO 3696 Grade II.
In order to obtain Grade I water, there is a recirculation loop connected to the tank.
For Trace modification this loop includes recirculation pump, polishing module (part no. 10030), Grade I
sensor as well as dispense port with microfilter (part no. 10012). HPLC and Bio configuration
additionally includes photooxidation module (part no. 10018). Crystal EX Bio system has an ultrafilter
(part no. 10120) instead of microfilter.

PRE-INSTALLATION
8
PRE-INSTALLATION
Make sure the pre-installation requirements are met before installing the system.
Feedwater requirements
Feedwater should be filtered with 1 μm sediment filter. If the filter is not installed, the input strainer
may become clogged thus blocking the water flow.
Check if sediment filter is installed in tap water
supply line. There should be at least one sediment
filter (pore size 1 µm). The sediment filter is
usually available from a local plumbing store.
If you cannot obtain a sediment filter locally, you
can order tap water line pre-filter set from Adrona
(see picture below). The part number is
10170 (carbonpp/ PP 1 μm) or
10171 (polyphosphate/carbonpp/1 μm).
Feedwater properties:
Type of feedwater Potable
Minimum pressure ≥0.4 bar
Maximum pressure ≤4 bar
Conductivity <1500 µS/cm
Temperature 5 – 35 °C
pH 4 – 10
Fouling Index <5
Iron <0.1 ppm as CaCO3
Aluminum <0.05 ppm as CaCO3
Manganese <0.05 ppm as CaCO3
Free Chlorine <1 ppm
Langelier Saturation Index <+0.2
TOC <2000 ppb
Feedwater connection
Feed water hardness does affect produced water quality. Operation of the system with hard feed water
may result in pre-mature clogging of reverse osmosis membranes and reduced Grade II water flow.
Therefore, it is strongly recommended to install a water softener or polyphosphate filter if
water hardness is above 160 ppm.
Feed water connection port has to be 1/2” NPTF (male) thread. The system is equipped with feed water
tube (1/4” OD) and adapter for 1/2” NPTF (female) water supply connection. The feed water tube
should be connected to the 1/4” John Guest port of the adapter. Feed water connection port should be
equipped with a valve allowing shutting off water supply.
Drain pipe should be lower that the level of the instrument and the level of the “OVERFLOW”
port of the tank. The water purification system has 1/4” OD drain pipe. The “OVERFLOW”
port of the tank is intended for 3/8” OD pipe with stem elbow 3/8”.
Feed water supply connector and drain should be within 3 meters from the installation site.

UNPACKING
9
Site requirements
The system requires up to 400*350*550 mm (W*D*H) space on the bench. If the system is equipped
with the tank, allow enough space for the tank. The tank can be placed under the bench.
Environmental requirements
The water purification system is intended for indoor use only, in an environment that has
nonconductive pollutants only.
Ensure that the site is maintained under the following conditions:
Condition Acceptable range
Temperature 15 to 30 °C (59 to 86 °F)
Humidity 20% to 80% relative humidity, noncondensing
Water leakage safety
Make sure that all water connection tubes are kink-free.
Make sure that all water connections are tight.
When installing the tank, connect the fitting marked “OVERFLOW” to the drain. Make sure
that the drain level is lower than the “OVERFLOW” fitting level. This will prevent water
leakage in case of tank level sensor failure.
A 1 um sediment filter has to be installed in the feedwater supply line. Failure to install the
filter may result in clogging of the strainer inside the system and water flow blockage.
Additional equipment
Water purification systems Crystal EX Trace, Crystal EX HPLC and Crystal EX Bio are not equipped with
internal tank.
Therefore, you may need to order external tank for purified water storage additionally. See Price list
for available options.
UNPACKING
Remove packing materials carefully and retain for them future shipment or storage of the unit. Examine
the unit carefully for any damage incurred during transit. The warranty does not cover in-transit
damage.
Due to the unit’s weight its unpacking and installing is to be carried out by two persons.
When opening the shipping box, we advise to compare received parts with the Packing List included.
Contact Adrona if any part is missing.

INSTALLATION
10
INSTALLATION
Preparation of the System
Review “Pre-installation requirements” chapter above.
Unpack the water purification system and place it on the laboratory bench.
Remove the protective packing materials.
Introduction to John Guest tubing system
Adrona water purification systems are equipped with John Guest push-fit fittings and tubes. We will
use abbreviation JG for John Guest parts in further text.
To make a connection, the tube is simply pushed in
by hand; the unique patented John Guest collet
locking system then holds the tube firmly in place
without deforming it or restricting flow
Push the tube into the fitting, to the tube stop
Pull on the tube to check it is secure. Test the
system before use
To disconnect, ensure the system is depressurized,
push the collet square against the fitting. With the
collet held in this position the tube can be removed

INSTALLATION
11
Rear and side panel
Look at the rear panel of the Crystal EX water purification system and locate pre-filter and
deionization module installation sites as well as inlet and outlet fittings.
Rear panel
1 – “REC IN” 1/4” connection
2 – “REC OUT” 1/4” connection
3 – “DRAIN” 3/8” connection
4 – “TAP WATER” 3/8” connection
Side panel
1 – Information about system & location of
the serial number
2 – Electric cable plug
3 – Power switch
4 – “TANK LEVEL” connection

INSTALLATION
12
Pro tank
The “Pro” 30 l tank with level sensor, base and tap (part no. 13003) is an optimal solution for purified
water storage. There are available other tanks with different volume. See price list for further
information.
Front view Rear view
Top close view Rear close view
1 – “TANK LEVEL” connector
2 – “OVERFLOW” tube output
3 – A cap
4 – Air filter
5 – Water tap
6 – “REC IN” connection to system
7 – “REC OUT” connection to system

INSTALLATION
13
Installation of filter
[1]
Remove protective cap from pre-filter
(part no. 10320)
[2]
Place pre-filter in the filter holder (i. e. filter head)
[3]
Install pre-filter at the rear panel of the water
purification system. To install the pre-filter, fit it
into the filter holder and turn it one quarter of a
turn

INSTALLATION
14
Installation of the modules
[1]
Locate the installation slots:
- deionization module (part no. 10310)
- polishing module (part no. 10030)
Installation process of the deionization
module and the polishing module is
identical
[2]
Locate the modules:
1 – deionization module (part no. 10310) is
marked with BLUE sticker;
2 – polishing module (part no. 10030) is marked
with SILVER sticker
[3]
Remove both protective ¼” JG plugs from
DI module.
Option 1.
You need to push the little ring down and you will
be able to take out the plug (see the principle in
chapter ”Introduction to John Guest tubing
system”)
[4]
You can use Adrotool for more convenient removal.
See Adrotool close view picture on the left

INSTALLATION
15
[5]
Option 2.
Protective 1/4” JG plug removal from DI module
using Adrotool.
You need to push the little ring down with Adrotool
and you will be able to take out the plug
[6]
Locate connectors for deionization module (JG
stem elbow with 1/4” tubes)
[7]
Insert connectors in the module fittings.
Make sure they are properly attached
[8]
Place deionization module in the slit

INSTALLATION
16
[9]
Place connector tubes in slots.
Make sure that connectors are properly attached
[10]
Repeat the same installation process with polishing
module.
Rear panel after installation of deionization and
polishing module is shown in picture on the left
Dispense filter mounting
[1]
Ultrafilter packaging (part no. 10120)
For Bio configuration only
[2]
0.22 m dispense filter packaging
(part no. 10012)
For HPLC and Trace configuration
only
[3] [4]
O-rings:
1 – yellow for ultrafilter
2 – black for 0.22 m dispense filter

INSTALLATION
17
[5]
For Bio systems, unpack the ultrafilter and screw
it to the holder in front of the system.
Before that, make sure that the yellow O-ring is
inside the holder
[6]
Ultrafilter connected with the system.
After water dispensing put the bell cap
back to the bell
[7]
For HPLC and Trace systems, unpack the 0.22 m
dispense filter and screw it to the holder in front
of the system.
Before that, make sure that the black O-ring is
inside the holder
[8]
0.22 m dispense filter connected with system.
After water dispensing put the bell
cap back to the bell
The microfilter (or ultrafilter) should be rinsed prior to use!
When the tank is full, press the “Dispense” button and rinse the microfilter (or ultrafilter)
with at least 10 liters (or 20 liters respectively) of purified water.

INSTALLATION
18
Connection of tubing
[1]
There are overall four 1/4” JG tubes in the set
[2]
Locate the 1/2” NPT thread adapter:
- Connect one 1/4” JG tube to the 1/2” NPT
thread adapter;
- Connect it to the feedwater source
[3]
Connect the feed water to the 3/8”–1/4” JG stem
elbow connection marked TAP WATER
[4]
Connect the 3/8”–1/4” JG stem elbow
connection marked DRAIN to the local drain
using 1/4” JG plastic tube
[5]
The next step is tank connection to the system:
1. locate REC IN and REC OUT connections
on the Crystal EX lower left side of the
rear panel [pic. 5];
2. locate REC IN and REC OUT connections
on the tank’s rear panel [pic. 6];

INSTALLATION
19
[6]
3. use two 1/4” JG plastic tubes for
connection [pic. 2];
4. connect REC IN on Crystal EX system
[pic. 5] with REC OUT on the tank [pic.
6] (marked with GREEN square);
5. connect REC OUT on Crystal EX system
[pic. 5] with REC IN on the tank [pic. 6]
(marked with BLUE circle)
[7]
Locate 3/8” JG tube with JG elbow adapter
[8]
Connect OVERFLOW port of the tank to the drain
using the 3/8” JG tube with JG elbow adapter
(3/8”).
When installing the tank, connect the
fitting marked “OVERFLOW” to the
drain. Make sure that the drain level is
lower than the “OVERFLOW” fitting level. This
will prevent water leakage in case of tank level
sensor failure.

INSTALLATION
20
Connection to tank cable
[1]
Locate the tank cable
[2]
Connect the tank cable to the “TANK LEVEL”
connector that is located on the top of the tank
[3]
Connect the tank cable to the “TANK LEVEL”
connector that is located on the side panel of the
system
[4]
Locate the power cable