Amerex Hirayama HG-50 User manual

HICLAVE
HG-SERIES AUTOCLAVES
(HG-50 and HG-80)
OPERATION MANUAL
Before using this Autoclave, please read this manual
and follow all Safety Rules and Operating Instructions.
3951C Industrial Way Concord, CA 94520
[phone] / [phone] ∙ Fax:[phone]
[e-mail] ∙ www.amerexinst.com

- 1-
Introduction
● We would like to express our gratitude for your purchase of our autoclave. This manual covers the
operation procedure and a simple maintenance method for the Autoclave HG-50 or HG-80 you now
own. We hope that owing to your proper handling, the autoclave can demonstrate its full
performance and that you will make regular use of it for a long time.
● For optional equipment (□ Recorder, □ Floating sensor, □ Automatic water supply unit,
□ Printer), if any, read the attached instruction manuals for the options.
● Please check whether or not the product conforms to your order and confirm that it was not
damaged during transportation. Should it be damaged or out of order, please contact our
authorized distributor in your region.
① No part or all of this manual may be reproduced without our permission.
② The contents of this procedure are subject to change without prior notice.
③ Although we tried hard to make this manual error free, if you find any unclear descriptions,
errors or omissions, kindly let us know.

- 2-
Read Carefully Before Using
● Determine the handling person responsible for this product.
● In this manual, the following headings are applied to items to which great attention should be given:
WARNING : Precaution indicating an imminent dangerous situation which if not
avoided may lead to death or serious injury.
CAUTION :Precaution indicating a dangerous situation which if not avoided
may lead to moderate or slight injury.
IMPORTANT : Indicates items you are strongly advised to obey.
△ NOTE : Items that will aid in proper operation of the equipment.
● Never use the autoclave to sterilize any of the following hazardous materials or substances with
alkali content. Sterilization of such objects can cause explosion, corrosion of the working
chamber or chamber piping, and deterioration of gaskets.
List of Hazardous Materials
① Explosive substances
・Nitroglycol, nitroglycerin, nitrocellulose, and other explosive nitric esters.
・Trinitrobenzene, trinitrotoluene, picric acid, and other explosive nitro compounds.
・Peracetic acid, methyl ethyl ketone peroxide, benzoyl peroxide, and other organic peroxides.
② Ignitable substances
・Metallic lithium, potassium, sodium, yellow phosphorous, phosphorus sulfide, and red phosphorus.
・Celluloids, calcium carbide (carbide), lime phosphide, and magnesium powder
・Aluminum powder, magnesium powder, and metallic powders other than aluminum powder
・Sodium dithionite (or sodium hydrosulfite)
③ Oxidizing agents
・Potassium chlorate, sodium chlorate, ammonium chlorate, and other chlorates
・Potassium perchlorate, sodium perchlorate, ammonium perchlorate, and other perchlorates.
・Potassium peroxide, sodium peroxide, barium peroxide, and other inorganic peroxides
・Potassium nitrate, sodium nitrate, ammonium nitrate, and other nitrates
・Sodium chlorite and other chlorites
・Calcium hypochlorite and other hypochlorites
④ Flammable substances
・Ethyl ether, gasoline, acetaldehyde, propylene oxide, carbon disulfide, and other substances
whose flash points range from -30 to 0C.
・Methanol, ethanol, xylene, benzyl acetate (or amyl acetate), and other substances whose flash
points range from 0 to 30C.
・Kerosene, gas oil, turpenine oil, isopentyl alcohol (or isoamyl alcohol), acetic acid, and other
substances whose flash points range from 30 to 65C.
⑤ Flammable gas (hydrogen, acetylene, ethylene, methane, ethane, propane, butane, and other
substances that are gases at a temperature of 15C under 1 atmospheric pressure.)
● When liquid with salt water and much salinity of salt agar etc. spills in the chamber, blowing,
discharge water in the chamber and wipe up drop of water around the lid gasket beautifully.
It causes the corrosion of the chamber and the piping when leaving just as it is.
● Check that the pressure is below "0 MPa" before opening the lid.
● Absolutely do not attempt to remodel or alter this product.
WARNING
0.4
0
０
Pressure
gauge

- 3-
● Foreign matter (metals, liquid) may enter through the vent hole. Operating the equipment with
such foreign matter inside may cause trouble with the equipment, fire or electric shock.
● Do not forcibly bend, twist, tie or extend the power cord. Do not place heavy objects on the cord.
A damaged cord or exposed wire can cause fire or electric shock.
● Never connect the power cord to a power supply other than one of the rated voltage. Connection
to such a power supply can cause fire or electric shock.
● If grounded socket is unavailable, ground the equipment using a separate ground wire before
connecting the power cord to the power source.
● Connect the grounding cable correctly to the Type D or higher-grade grounding terminal.
Never connect the grounding wire to gas pipes or water pipes.
● Close the lid after confirming that no foreign matter is adhering to the section contacting the lid
gasket. Foreign matter in this section can cause vapor leak.
● When using a waste processing bag or other kind of bag and sterilizing, place the bag in the wire
basket and then insert it into the chamber. Using the bag “as is” can cause excessive
temperature, excessive pressure, boil dry, etc..
● Do not contact the mouth of the container which is placed in the chamber with the inner surface
of the lid. If the mouth of the container is closed by the lid inner surface, gas or liquid will gush out
of the container when opening the lid
● Be careful not to pinch your hands when closing the lid.
● Do not touch the lid or lid cover when opening or closing the lid.
● Do not put your face or hands close to the chamber when lifting the lid immediately after
completion of the operation; steam will gush out of the chamber.
● The lid, chamber, gasket and panel are extremely hot after completion of the operation.
Do not touch the equipment or you may get burned.
● Put on heat insulating gloves before removing a substance from the chamber. Do not put hands
into the chamber until the steam has been vented.
● Some time is required for liquids to cool. Be sure to check that the temperature has dropped
sufficiently before unloading a liquid from the chamber or burns can result.
● If the liquid surface which is placed in the chamber is covered with oil, etc, the liquid may gush
out when taking out or carrying. Be sure to check that the temperature has dropped sufficiently
before unloading the liquid from the chamber or it may cause burns.
● Do not unload the drain bottle or drain the chamber when the chamber is under pressure.
Boiling water or steam may gush out and it may cause burns.
● Do not remove the drain bottle before water in the bottle has sufficiently cooled down.
● If any abnormality occurs (e.g. abnormal sounds, smells, smoke), immediately shut the main
power off. After checking to see that the abnormal condition does not continue, call our
authorized distributor in your region.
● If any abnormality in display occurs, turn the main power switch off then on again. If the problem
continues, turn the main power switch off and call our authorized distributor in your region.
● Do not apply force to the lid that is open. Such force may damage the product.
CAUTION
IMPORTANT

- 4-
How to Read this Manual
● This operation manual consists of the following sections covering the information required for
proper operation of the Autoclave HG-50 or HG-80.
Chapter 1. What is the Autoclave HG-50, HG-80?
This section describes the uses and features of the product and the names and functions of its
parts.
Chapter 2. Installation
This section explains where the equipment should be installed and how to install it. The product
incorporates precision parts, so be sure to follow the instructions covered in this chapter.
Chapter 3. Operation Method
This section illustrates how to change various set values, and describes operations before starting
the equipment and after automatic operation. This section also covers the display and performance
of the equipment during automatic operation.
Chapter 4. Maintenance and Service
This section explains the methods for draining water from the drain bottle or chamber, cleaning the
body of the equipment, and parts replacement.
Chapter 5. Specifications
This section includes dimensions, power consumption and working range of the product. Refer to
this section as is required.
Chapter 6. Troubleshooting
This section covers troubleshooting procedures for the product. If you encounter a problem, read
this section first.
Appendix
This section contains information on the warranty and a glossary of terms that appear in the manual.
Please refer to this section when necessary.

- 5-
CONTENTS
Introduction ...................................................................................................... 1
Read Carefully Before Using ........................................................................... 2
How to Read this Manual ................................................................................. 4
CONTENTS ....................................................................................................... 5
Chapter 1. What is the Autoclave HG-50,HG-80? .......................................... 6
1. Product Uses .................................................................................................................... 6
2. Product Features .............................................................................................................. 6
3. Names and Functions of Each Part .................................................................................. 6
Chapter 2. Installation ...................................................................................... 9
1. Installation instructions ..................................................................................................... 9
2. Installation Procedure ..................................................................................................... 10
Chapter 3. Operation Procedure ................................................................... 13
Basic Operation Procedure ................................................................................................ 13
1. Power Source ................................................................................................................. 14
2. Pouring Water ................................................................................................................. 14
3. Placing the Load ............................................................................................................. 15
4. Selection of a Mode and Program .................................................................................. 16
5. Changing Set Values (Registration of the values by a user) .......................................... 18
6. Changing Function Setting ............................................................................................. 21
7. Reservation Operation .................................................................................................... 23
8. Starting Operation .......................................................................................................... 25
9. Taking Out the Load ....................................................................................................... 26
10. After Completion of Operations .................................................................................... 27
11. To Interrupt Operation ................................................................................................... 27
12. If Power Supply is Cut Off during Operation ................................................................. 27
13. Operation of Each Stage .............................................................................................. 27
Chapter 4. Maintenance and Service ............................................................ 31
1. Draining the Drain Bottle ................................................................................................ 31
2. Draining the Chamber .................................................................................................... 32
3. Cleaning inside the Chamber ......................................................................................... 32
4. Cleaning Body ................................................................................................................ 33
5. Water Supply to Cooling Tank ........................................................................................ 33
Chapter 5. Specifications .............................................................................. 34
Chapter 6. Troubleshooting .......................................................................... 35
1. Error Detection (Alarms) ................................................................................................. 35
2. Early Troubleshooting ..................................................................................................... 36
Appendix ......................................................................................................... 37
1. Consumable parts .......................................................................................................... 37
2. Glossary ......................................................................................................................... 37
3. Warranty ........................................................................... Error! Bookmark not defined.

- 6-
Chapter 1. What is the Autoclave HG-50,HG-80?
1. Product Uses
・The product is used to sterilize the load which can withstand high temperature and high pressure
steam such as tools of glass, ceramic, metal or rubber, water, media, reagents and liquid medicines
(Liquid, Agar, Solid modes) .
・The product is also used to liquefy media (Dissolution mode).
2. Product Features
・In order to secure safety, the product is provided with a cover around the section that becomes hot
during operation.
・The lid of the product is opened and closed automatically. So, it is not necessary to bring your hands
near the upper part of the chamber to open or close the lid. It enhances the safety of the equipment.
・The agar cycle, which prevents solidification of sterilized media when they are not taken out
immediately, and the dissolution mode, which dissolves solidified media, are provided.
・When the exhaust level (the opening ratio of the exhaust valve) is set, fine exhaust is conducted
automatically after sterilization.
・Steam that comes out of the exhaust valve during air release and exhaust is cooled in the cooling
tank to minimize the quantity of steam that is emitted into the room.
・This equipment can set the temperature, at which the load cannot be taken out, between 60℃and
97℃ in each cycle and each program number for safety operation
・A reservation timer is provided to enable operation startup at any desired time within the range of
1 hour to 99 hours.
3. Names and Functions of Each Part
● HG-50 External View

- 7-
● HG-80 External View

- 8-
● Operation panel
① Digital Display (Temperature)
The digital display indicates the preset sterilization temperature, etc. in the standby state, and it
indicates the chamber temperature during operation.
② Digital Display (Time and Pressure)
The digital display indicates the set time, reservation time, etc. in the standby state, and it indicates
the pressure inside the chamber, time remaining until completion of sterilization, and time
remaining until completion of warming during operation.
③ Digital Display (Warming)
The digital display indicates the warming temperature, function number, etc. in the standby state,
and it indicates the error when it is detected.
④ Unit Display ( , , , and )
The unit corresponding to the current numeric value lights up.
⑤ State Display ( , , and )
The state display indicates the present state.
⑥ Stage Display
All the stages included in the selected cycle light up, and the current operating stage flashes.
⑦ Drain Bottle Display
The red lamp blinks when the drain bottle is full, and the green lamp illuminates when it is not full.
⑧ POWER Switch
Used to turn on or off the power to the autoclave.
⑨ LID CLOSE Switch
Used to close the lid.
⑩ LID OPEN switch
Used to open the lid. When the lid is in the state to be opened, a green light comes on, and when
the lid cannot be opened because the chamber is hot and highly pressurized, a red lamp lights up.
⑪ Mode Switch
Used to select the mode and program number.
⑫ FUNC. Switch
Used to change and confirm the setting of respective functions.
⑬ SET switch
Used to change and confirm the set value.
⑭ Set Value Increase/Decrease Switch (▲ ▼)
Used to increase or decrease the set value.
⑮ Exhaust Level Switch (◄►)
Used to change the exhaust level.
⑯ FAN Cooling ON/OFF Switch
Used to change fan cooling ON/OFF setting.
⑰ START Switch
Used to start operation.
⑱ STOP Switch
Used to stop operation.
⑲ PROGM Display
The display indicates the present program.

- 9-
Chapter 2. Installation
● If the equipment is installed in a place which is 800m or higher than sea level (i.e. under low
pressure in mountainous areas), the settings must be changed. In this case, be sure to contact
our authorized distributor in your region. Do not use the equipment before changing the
specification.
● When relocating the product, close the lid so that it will not move, and then remove the drain
bottle and power cord.
1. Installation instructions
①Avoid installing the equipment in a place where its
body may be exposed to water or chemicals, or
where corrosive and explosive gases may be
produced neareby.
③Avoid placing the equipment directly under a fire
detector. If you open the lid immediately after
completion of operation, steam comes out of the
working chamber, and may activate the detector.
④Arrange the equipment with a clearance of 10 cm
or wider on the right side and 12 cm or wider on
the rear side to prevent the vent hole from
being blocked.
10cm or more
⑤Avoid an installation place which is subject to impact
or vibration.
⑥Place the unit in a level, firm place which can withstand
the load with 120 kg or above.
⑦Avoid installing in a place which is subjected to a room
temperature of 5
℃
or below or 35
℃
or above.
⑧Avoid shining an ultraviolet [UV] lamp on resin parts
due to cause change of color.
②Avoid installing the equiopment in a place which is
exposed to hight humidity, direct sunlight or much
dust.
0.2
0.3
0. 40
0. 1 0.2
0.3
0. 40
0. 1
0. 2
0.3
0. 4
0
0. 1
10cm or more
12cm or more
IMPORTANT

- 10 -
2. Installation Procedure
① Pour water into the cooling tank.
・Remove the cap from the water supply port
at the back of the unit, and pour water through
the water supply port by using a hose, etc. up to
the center of the level check window at the side
of the unit.
After water supply, return the cap back to the
original position. (Hoses, etc. are not provided.
Please prepare them by yourself.)
② Connect the power cord to the rated power supply.
・Be sure to connect the grounding wire.
● Do not forcibly bend, twist, tie, or extend the power cord. Do not place heavy objects on
the cord. A damaged cord or exposed wire may cause fire or electric shock.
● Never connect the power cord to a power supply with a voltage other than the rated
voltage. Connection to such a power supply may cause fire or electric shock.
● If not plugging the sterilizer into a grounded socket, ground the equipment separately
before connecting it to the power source.
● Never ground to a gas pipe or vinyl chloride water service pipe.
・Connect the unit to the rated power source as specified below and ground the Green/Yellow
earth wires.
HG-50 :
・AC220V /AC230V: 14 A or more, AC240V : 13 A or more.
・When the 2kw heater is used as option,
AC110V: 19 A or more, AC120V: 17 A or more,
AC220V: 10 A or more, AC230V/AC240V: 9 A or more
HG-80 :
・AC220V: 17.3 A or more, AC230V: 16.6 A or more, AC240V: 15.9 A or more
CO N NECT TO RATED VOLTAGE
Black
White
Green/Yellow
Connect to ground
Water supply port
Cap
Center
Waterlevelcheckwindow
CAUTION

- 11 -
③ Turn ON the main power switch.
・Raise the lever of the main power switch in the right side of the unit.
Raise.
Main power switch
④ Referring to “Chapter 3, Operation Procedure”, open the lid and take out the accessories.
then, turn OFF the main power switch.

- 12 -
⑤ Put casters on the caster stoppers so that the unit will not move.
・Place two caster stoppers on a point which is distant
from a wall 64cm or more, and push the unit so that the
front casters will be put on the stoppers.
・Be sure to set the caster stoppers, otherwise the
full water detecting function of the drain bottle may
not be activated.
⑥ Place the bottom plate in the chamber.
⑦ Attach the drain bottle.
・Check that there is no foreign material or irregularity on the floor where the drain bottle
is to be set. (Otherwise, the full water detecting function may not be activated.)
⑧ Turn ON the main power switch again.
64 cm or more
Caster stopper

- 13 -
Chapter 3. Operation Procedure
Basic Operation Procedure
Turn “ON” Power Switch
――――――
See "1. Power Source” on page 14.
▼
Open the lid
▼
―――――
See "2. Pouring Water" on page 14.
Pour water
▼
Place the load
――――――
See "3. Placing the Load" on page 15.
▼
Check and select a mode
See "4. Selection of a Mode and Program" on page 16.
▼
Check and change set
values
See "5. Changing Set Values" on page 18.
▼
▼
(When
starting operation by a reservation timer)
See “7. Reservation Operation”
on page 23
Start operation
See "8. Starting Operation" on page 25.
▼▼
Check if the operation is
completed
▼
Open the lid
―――――
See "9. Taking Out the Load" on page 26.
▼
Take out the load
▼
Turn “OFF” Power Switch
――――――
See "10. After Completion of Operations" on page 27.
Set a reservation timer

- 14 -
1. Power Source
① Press the "POWER" switch on the operation panel.
・The light comes on, the preset value is displayed, and the unit enters the standby state.
・When none of the operation switches is touched for 10 minutes, the power saving function is
activated, the display light goes out, and a dot blinks on the digital temperature screen.
Press any switch other than the "LID OPEN" switch, and the unit returns to the standby state.
● When the power supply is interrupted or the main power switch is turned OFF, the temperature
and time set for the previous operation will appear.
2. Pouring Water
● When the sterilization time is extended or a large quantity of cloth is put in, pour a larger quantity
of water than specified.
① Press the "LID OPEN" switch.
・The lid will open automatically.
② Pour water until water rises through the center hole of the
bottom plate.
・When there is no water at all, pour 3 liters of water for
HG-50 and 3.5 liters for HG-80. Supply of water is
also necessary for the dissolution cycle.
△ NOTE
IMPORTANT
P R E S S
T I M E
PROGM 1
TEMP
2 3
℃
MPa
Hr/min W A R M
℃

- 15 -
3. Placing the Load
● When using a waste processing bag or other kind of bag and sterilizing, place the bag in the wire
basket and insert it into the chamber. Using the bag “as is” can cause excessive temperature,
excessive pressure, boil-dry, etc.
● Do not contact the mouth of the container which is placed in the chamber with the inner surface
of the lid. If the mouth of the container is closed by the lid inner surface, gas or liquid will gush out
of the container when opening the lid.
● Be sure to use the bottom plate.
● The mating surface of the chamber to the gasket is important for gas sealing.
Exercise care so as not to damage the section by hitting it with loaded objects.
● Do not put in any object longer than the depth of the chamber stated in the specifications.
① Put the objects to be sterilized
(dissolved material) in the chamber.
● When sterilizing an empty deep container, lay the container on its side in the chamber so that it
will be permeated with steam. An upright position may cause insufficient sterilization.
● If a waste disposal bag is used in sterilization, open the bag properly so that the bag is not in
contact with the inside surface of the chamber. Insufficient sterilization may be caused if the bag
is closed during sterilization. When the bag is opened excessively, steam is prevented from
circulating in the chamber. This may also result in insufficient sterilization.
● Do not pile up the sterilization bucket in the chamber. When buckets are excessively piled, steam
fails to penetrate to all points, resulting in incomplete sterilization.
● In sterilizing liquids such as medical solution and media, pay attention to the quantity of the liquid
in relation to its container. For an Erlenmeyer flask, the amount of liquid should be approx. 3/4 of
the capacity of the container; for a test tube, the appropriate quantity of chemical is approx. half
of the capacity of the container. Too much chemical may result in overflow from the container
during the temperature rising or cooling process.
● Use container caps that are loose fitting and allow the passage of air. Containers may break if
venting is not possible.
● In the case of dissolution of agar media, its quantity should be 2 liters or less per container.
Two liters or more of agar medium may not be completely dissolved.
● Use the DURHAM TEST TUBE (Sample tube) with 6mm caliber or more. At the DURHAM TEST
TUBE (Sample tube) with less than 6 mm caliber, air bubble sometimes remains. Delaying of air
release time may allow no air bubbles to be left. Change the air release time by referring to
“6. Change of Function Setting-Air release time.”
CAUTION
IMPORTANT
△ NOTE

- 16 -
4. Selection of a Mode and Program
● Select a mode according to the usage.
① Press the mode switch (LIQ, AGAR, SOLID, or
DISSOL).
・Every time the switch is pressed, the current mode
changes from Mode 1 to Mode 2, 3, 1, ・・・
in sequence repeatedly.
Mode
Application
Liquid
Sterilization of water, media, solid reagent, liquid reagent, liquid chemical, and
other liquid that can withstand high-temperature high-pressure steam.
Agar
Sterilization of agar media (to be warmed so that it will not solidify after sterilization)
Solid
Sterilization of glass, ceramics, metal, rubber, and other solid equipment that can
withstand high-temperature high-pressure steam and sudden decompression
during exhaust
Dissolution
Dissolution of media
Liquid mode
Stage display
Initial set value (for all 3 programs)
Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warm
temp.
HEAT → STERI →
EXHT./COOL
121℃
20 min.
0
OFF
Agar mode
Stage display
Initial set value (for all 3 programs)
Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warm
temp.
HEAT → STERI →
EXHT./ COOL → WARM
121℃
20 min.
0
OFF
50℃
AGAR
LIQ.
SOLID
DISSOL

- 17 -
Solid mode
Stage display
Initial set value (for all 3 programs)
Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warm
temp.
HEAT → STERI →
EXHT./COOL
121℃
20 min.
3 (fixed)
ON
(fixed)
Dissolution
mode
Stage display
Initial set value (for all 3 programs)
Sterilizati
on temp.
Sterilizati
on time
Exhaust
level
Fan
cooling
Warm
temp.
HEAT → DISSOL →
COOL → WARM
100℃
10 min.
OFF
50℃

- 18 -
5. Changing Set Values (Registration of the values by a user)
● When power supply is interrupted or the main power switch is turned OFF before starting the
operation with the preset temperature and time, the preset values will return to the original
values.
Select the mode for which the setting is to be changed, select the program number under which the
set values are to be saved, and then change the setting as shown below.
● Sterilization temperature, sterilization time, warming temperature, dissolution temperature,
and dissolution time
The set values cannot be changed during operation.
① Press the "SET" switch.
The set sterilization (dissolution) temperature
display blinks, permitting setting change.
② When changing the setting other than the
sterilization (dissolution) temperature, press the
"SET" switch further and select the setting item
to change.
・Every time the switch is pressed, the alterable setting items vary in sequence as shown below.
Switch input
▽ ▽ ▽
Liquid mode
Sterilization
temperature
Sterilization
time
Agar mode
Sterilization
temperature
Sterilization
time
Warming
temperature
Solid mode
Sterilization
temperature
Sterilization
time
Dissolution
mode
Dissolution
temperature
Dissolution
time
Warming
temperature
③ Press the set value increase/decrease switch (▲ ▼) to change the value.
・Every time the switch is pressed, the set value increases or decreases as follows;
Sterilization temperature : in 1℃ increments within the range of 105℃ to 135℃
Sterilization time : in 1 minute increments within the range of 1 min. to 5 hours
Dissolution temperature :in 1℃ increments within the range of 60℃ to 100℃
Dissolution time : in 1 minute increments within the range of 1 min. to 1 hour
Warming temperature : in 1℃ increments within the range of 45℃ to 60℃
・While the switch is kept pressed, the value increases ( decreases ) at the rate of 10 units,
and it will return to the lower (upper) limit when the value exceeds the upper (lower) limit.
④ If the switch is left untouched for 5 seconds, the display will return from blinking to steady state,
and the setting is completed.
△ NOTE
SET
SET
SET

- 19 -
● For sterilization of liquid, set a sterilization time longer than usual by taking delay time into
account and by referring to the table below.
Ex. : When there is 3 liters of water in a flask, it takes nearly 30 minutes (delay time) for
temperature of water in the container to reach a set sterilization temperature after
temperature in the chamber reaches the set value. You should set a sterilization time
30 minutes longer than usual to cope with this delay time.
Set sterilization time (50 minutes)
= Delay time (30 minutes) + Sterilization time (20 minutes)
Reference Values of Delay Time
(per Flask)
Liquid Volume
Delay Time
3 liters
30 minutes
2 liters
25 minutes
1 liter
20 minutes
500 cc
15 minutes
● When a dressing drum is used, it takes several hours for the temperature in waste disposal bag
to reach the set temperature after the temperature in the chamber (displayed temperature)
reaches the set temperature (delay time).
If there is approx. 300 ~ 500 milliliters of water in the waste disposal bag, steam is generated in
the bag and drives the air out. This will significantly reduce the delay time at the time of
temperature rise. Refer to the table below and take this delay time into account when setting
the sterilization time.
The delay time will be shortened when a perforated dressing drum is used.
Reference Values for delay time in Bag
Water in Bag
Delay time
Case: A large number of φ15 x 100 test tubes
placed in a waste disposal bag.
Not poured
206 minutes
Poured
48 minutes
● For dissolution of coagulated agar medium, set an appropriate dissolution temperature and time,
referring to the table below.
Reference Values (per Flask)
Quantity of Liquid
Dissolution Temperature
Dissolution Time
2 liters
100℃
60 minutes
1 liter
45 minutes
500 cc
25 minutes
△ NOTE
40
60
80
100
120
12 0
(Temperature)
(Time)
Set sterilization time
Delay time Sterilization time
Temperature of water
Temperature
Delay Time Reference Data
in 3 liter container
temp.
R oom
in working chamber