VWR SAS Super IAQ User manual

VWR® Microbiological Air Sampler
SAS Super IAQ
INSTRUCTION MANUAL
European catalogue numbers:
710-0868 710-0919
Version: 1
Issued: February 2020

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Legal address of manufacturer
Europe
VWR International Srl
Via S. Giusto 85
20153 Milano
Italy
Tel.: + 39 02-3320311
http://it.vwr.com
Country of origin: Italy

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Table of contents
Legal address of manufacturer ................................................................................................ 2
Warning ..................................................................................................................................... 4
Safety information .................................................................................................................... 4
Kit and unit description ............................................................................................................ 5
Technical features ..................................................................................................................... 6
Intended use .............................................................................................................................. 6
Brief operating instructions ..................................................................................................... 7
Installation ................................................................................................................................. 7
Functions ................................................................................................................................... 9
Colony Forming Unit correction factor .................................................................................. 15
Accessories ............................................................................................................................. 18
Troubleshooting ...................................................................................................................... 19
Technical service .................................................................................................................... 20
Warranty .................................................................................................................................. 20
Compliance with local laws and regulations ......................................................................... 20
Equipment disposal ................................................................................................................ 21

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Warning
This document is the property of VWR International S.r.l. - Milan - Italy
It may not be duplicated or distributed without the owner’s authorisation.
Patent pending
Manufactured by VWR International S.r.l. - Milan, Italy
Safety information
Use the device only for the indicated purposes.
The device must be correctly used according to this instruction manual before starting any operation.
Immediately replace any electrical cable when damaged.
Always disconnect the charger before:
 Repairing or maintenance; these operations must be carried out by qualified staff
 Cleaning the unit
Use original spare parts and accessories for any replacement.
Do not use this device in the presence of explosive gas.
Please follow the guidelines below and read this manual in its entirety to ensure safe operation of the unit.
Be aware that the voltage and frequency of the electrical system are compatible with the electrical
requirements of the battery charger.
Never use a non OEM charger to charge the air sampler.
Use of improper charger may damage the unit.
.
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Kit and unit description
1. Soft carrying case
2. SAS IAQ control unit
3. Aluminium head
4. Battery charger
5. Remote control
6. “Clear” button
7. “Enter” button
8. Arrow “Up”
9. “Start” button
10. Arrow “Down”
11. Standing feet
12. Printer connection (not used)
13. Main switch
14. Serial number
15. CE mark
16. Charger connection
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Technical features
Model SAS IAQ Contact SAS IAQ Petri
Cat. No. 710-0868 710-0919
Airflow 100 l/min 100 l/min
Use with 55 mm contact plates 90 mm Petri dishes
Portable Yes Yes
Battery Rechargeable Rechargeable
Battery life 70 000 l 70 000 l
Intended use
Principle
The Surface Air System (SAS) encompasses several models which use the same principle. Air is aspirated at a fixed
speed for variable time through a head which has been machined with a series of specially designed small holes.
The resulting laminar airflow is directed onto the agar surface of a “RODAC plate” (or Petri dish) containing
medium for microbiological analysis.
When the selected sampling cycle is completed, the plate is removed and incubated. The organisms are then visible
to the naked eye and can be counted for the assessment of contamination levels.
The basic idea
The key features of the Surface Air System (SAS) are:
1. To use a simple and inexpensive “contact plate” (“SURFAIR PLATE”, “RODAC”) for surface, hand or air
control. These plates are very well known and easily available. They can be purchased ready poured with
different media.
2. To sample a known volume of air for a variable time to provide a range of sampling volumes.
3. To aspirate air in a laminar flow pattern with sufficient velocity to impact organisms onto an agar surface.
4. To accumulate data on the level of hygiene in each environment so that fluctuations can be monitored.
5. To take advantage of advanced electronics for more reliable results in different operating conditions.
6. To have the flexibility to choose between 55 mm contact plates or 90 mm standard Petri dishes.
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Brief operating instructions
Brief operating instruction for SAS Super IAQ
1. Press main switch.
2. Press ‘START’ button to sample the same air volume as the last sampling cycle.
3. To change the air volume, use the arrow buttons and select ‘USER MODE’ from the menu.
4. Refer to the instruction manual to change settings.
5. Press ‘ENTER’ to confirm selection.
6. Press arrows for menu selection to modify other pre-set parameters.
7. Press ‘ENTER’ to reach the sub menu of the chosen parameter.
8. Refer to the instruction manual to change settings.
9. Press ‘ON/OFF’ switch at the end of sampling operations.
10. Press ‘CLEAR’ each time you need to end an action. The unit will then return to the initial configuration.
Installation
Practical use of contact plates
1. Remove the aspirating head.
2. Insert an identified, closed and prepared plate and remove the plate lid.
3. Replace the aspirating head.
4. Select required air volume and start the unit. The airflow is directed onto the agar surface of the plate.
5. At the end of the cycle, remove the aspirating head.
6. Close and remove the plate.
7. Incubate.
8. Count the colonies, record the results on the microbiological air sampling report and read the results.
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List of menus and utility sub menus
START FOR XXX This message indicates the air sampler is ready to sample the volume shown
(XXX) after pressing the ‘Start’ button
USER MODE Choose from 8 programmable sampling volumes
PROGRAM MODE Modify the 8 programmable sampling volumes
DELAY MODE Set a delayed start
UTILITY MODE This leads to the following sub menu:
SET TIME
To adjust the date and time
SET AUTOSWITCH
To disconnect the automatic switch-off when the “Infrared Remote” is used
DISPLAY RECORD
To show the recorded sampling data
CLEAR RECORD
To clear the recorded sampling data
LANGUAGE
To display text in different languages
IDENTIFY
To identify the operator’s name
SAMPLING SITE
To identify the sampling point
Preliminary inspection
The instrument is subject to specific quality tests before shipment and is carefully packed to avoid possible damage
during transit. However, a visible check should be carried out as soon as possible to determine any damage. This
must be reported immediately. The following procedures should be followed to check that the unit is working properly.
The instrument battery pack must be charged for at least 4 hours before running the test.
After the ‘ON/OFF’ button is pressed an automatic visual presentation will be displayed.
Holder adjustment
Contact plate and Petri dish holders can be adjusted (using a screw driver) in case the available plate is slightly
different in diameter from the standard 55 mm contact plate or 90 mm Petri dish.
Petri head adapter (optional)
An adapter is available in aluminium or stainless steel which allows the use of standard 90 mm Petri dishes with
SAS for contact plates.
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90 mm Petri dish filling
We recommend you fill the standard 90 mm disposable Petri dish with no more than 18 - 20 ml of agar to avoid the
medium touching the inside surface of the aspirating head.
Tripod installation (optional)
The SAS SUPER IAQ can be fixed to a table, floor tripod or wall support. The screw thread connection is located
under the unit, between the two front feet.
Functions
Software
Press the ‘ON/OFF’ switch (black button) to switch the unit on.
The ‘ON/OFF’ switch (black button) must be pushed twice to switch on the unit if the automatic switch-off timer was
used previously.
Press ‘CLEAR’ at any time to return to the initial ‘START FOR’ main screen.
The air sampler is provided with eight programmable air volumes (‘User Mode’) selectable by the operator.
Volume selection is made by pressing the “▲” or “▼” arrows when the programme is in the relevant sub menu.
Suggested air volumes:
- Contaminated areas (communities, processing rooms, etc) 10 - 200 litres of air
- Normal areas (laboratory benches, houses, etc) 200 - 500 litres of air
- Sterile or high-risk areas (cleanrooms, operating theatres, etc) 500 - 1000 litres of air
Starting with the same air volume as the previous sample
Switch on the unit by pushing the main switch. You will then see the following display with the last aspirated volume
of air:
Push the ‘START’ button to start a sampling cycle with the displayed volume of air.
‘USER MODE’
Up to eight additional volumes can be programmed into the unit. User-selectable volumes can be selected from the
‘USER MODE’, while the selectable volumes can be modified using the PROGRAM MODE’ function described in the
next paragraph.
Switch on the instrument and wait until the main screen is displayed:
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Press the “Up” or “Down” arrows to reach the:
Press the ‘ENTER’ button, now you can scroll the eight user-defined sampling volumes.
Once the desired sampling volume has been reached press the ‘ENTER’ button to confirm.
Press the ‘START’ button to begin the sampling cycle:
There are a total of eight available ‘“USER MODE’ programmes.
The maximum volume of air for each sampling cycle is 2000 litres.
‘PROGRAM VOLUME’ (modification of the value of a stored volume)
Using this procedure, it is possible to memorise up to eight different volumes (from 1 to 2000 litres of air). Switch on
the instrument and wait until the main screen is displayed.
Press the “Up” or “Down” arrows to reach the:
Press the ‘ENTER’ button, now you can scroll the eight user-selectable sampling volumes.
Select a volume by pressing the “UP” or “DOWN” arrows and then pressing ‘ENTER’ (value starts flashing):
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Change the digit indicated by pressing the “UP” or “DOWN” arrows and presses OK to go to the next figure.
Repeat until you reach the last digit and then confirm by pressing OK.
Once the sampling volume has been set, press the ‘ENTER’ button to confirm.
The display will show the main screen with the last aspirated volume of air.
‘DELAY MODE’
With this function it is possible to delay the beginning of the sampling cycle. If a delay has been selected, the sampling
cycle will begin after the selected delay time.
Switch on the instrument and wait until the main screen is displayed:
Press the “Up” or “Down” arrows to reach the:
Press the ‘ENTER’ button and the delay time is displayed:
Select the desired delay time using the “Up or “Down” arrows. The selectable delay values are 1, 2, 3, 4, 5, 6, 7, 8,
9, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55 or 60 minutes. Press the ‘ENTER’ button to confirm.
When a delay has been selected, after the “START” button has been pressed, a delay warning message will be
displayed.
‘UTILITY MODE’
With this function it is possible to modify all the settings of the air sampler.
The ‘UTILITY MODE’ is divided into seven sub menus:
Set Time; Set Autoswitch; Display Record; Clear Record; Language; Identify; Sampling Site.
Always start from ‘UTILITY MODE’ to reach one of these sub menus.
Switch on the instrument and wait until the main screen is displayed:
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Press the “Up” or “Down” arrows to reach the:
Press the ‘“ENTER’ button, the first sub menu is then listed.
‘SET TIME’
This option is used to programme day, month, year and time of day.
Press ‘ENTER’ and the “Up” or “Down” arrows to change the month
Press ‘ENTER’ and the “Up” or “Down” arrows to change the day
Press ‘ENTER’ and the “Up” or “Down” arrows to change the year
Press ‘ENTER’ and the “Up” or “Down” arrows to change the hours
Press ‘ENTER’ and the “Up” or “Down” arrows to change the minutes
Press ‘ENTER’ to confirm and exit
‘SET AUTOSWITCH’
To save battery consumption the SAS automatically switches off after 4 minutes. The ‘SET AUTOSWITCH’ option is
used to enable or disable the automatic switch-off.
From the ‘UTILITY MODE’ select ‘SET AUTOSWITCH’:
Press ‘ENTER’ and the “Up” or “Down” arrows to change between the two options:
Select the desired options and press’ ENTER’ to confirm and exit.
This setting will be lost after the instrument is switched off.
‘DISPLAY RECORD’
The last 99 samples are memorised in the file ‘DISPLAY RECORD’. Each sample is identified in chronological
order and shows the date, time, operator, site and volume of air sampled.
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From the UTILITY MODE select ‘DISPLAY RECORD’.
Press ‘ENTER’ to see the memorised values:
In the placeholder symbolized by a red box the following symbols can be mentioned:
Single head Dual head
Error X X
Single run l, r, b
Multi Cycle * L, R, B
The following parameters are recorded:
Progressive number; Month and Day; Time; Identification/Site; Sampling Volume.
If there is no data stored in memory the display will show:
‘CLEAR RECORDS’
This option is used to delete all the data memorised in the DISPLAY RECORD”. Before starting this procedure,
please be certain that existing data is not required or that it has been downloaded.
From the UTILITY MODE select ‘CLEAR RECORD’.
Press ’ENTER’ to delete all the stored data,
Press ‘ENTER’ to confirm the deletion of data or press CLEAR to exit the function.
The display will show the resetting status:

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‘LANGUAGE’
Menu text may be selected by choosing from different languages.
From the UTILITY MODE select ‘LANGUAGE’:
Press ‘ENTER’ to list all the available languages:
Use the “Up” or “Down” arrows to select the desired language and press ‘ENTER’ to confirm.
There are six available languages:
English, German, Spanish, French, Italian and Portuguese.
‘IDENTIFY’
This option is used to identify the operator. This should be changed if different operators use the sampler and
especially if the data is to be printed.
From the UTILITY MODE select ‘IDENTIFY’.
Press ‘ENTER’ to modify identification code:
Select the desired character using the “Up” or “Down” arrows and confirm with ‘ENTER’.
Select the next desired characters and confirm them by pressing ‘ENTER’.
The selected values are stored in the memory and will be kept even after the instrument has been switched off.
‘SAMPLING SITE’
Site identification should be changed for samples taken at different sites, especially if the results are to be printed.
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From the UTILITY MODE select ‘SAMPLING SITE’.
Press ‘ENTER’ to modify:
Select the desired character using the “Up” or “Down” arrows and confirm with ‘ENTER’. Select the next desired
characters and confirm them by pressing ‘ENTER’.
The selected values are stored in the memory and will be kept even after the instrument has been switched off.
List of system messages
Besides the messages described in the previous paragraph, the following messages can be displayed:
**LOW BATTERY** Battery must be recharged
MOTOR ERROR Motor not working or not connected
- OFF The instrument is switching off
Colony Forming Unit correction factor
The number of organisms counted on the surface of the dish must first be corrected for the statistical possibility of
multiples particles passing through the same hole. The statistical formula is taken from work by J. Maker.
Correction Tables are given for both the 55mm standard contact head and the 90mm Petri head. The probable count
(Pr) is then used to calculate the number of CFU per cubic meter of air sampled.
Example of calculation of results
X = Pr ∗ 1000
V
Where:
V = Volume of sampled air = 200 litres of air
r (see table) = Colony Forming Units counted on “55 mm Contact Plates”= 67
Pr = Probable count obtained by table positive hole correction = 80
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X = Colony Forming Units per 1000 litres (= 1 cubic meter) of air
So:
X = ∗
 400 CFU per 1000 litres of air (1000 litres=1m3)
To express the final result in CFU/ft3 multiply the CFU/m3 value by 0,02832

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r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
r
Pr
1 1 38 42 75 92 112 156 149 249 186 412 1 1 41 43 81 90 121 144 161 206 201 278
2 2 39 43 76 93 113 158 150 252 187 418 2 2 42 44 82 92 122 145 162 207 202 280
3 3 40 44 77 95 114 160 151 255 188 425 3 3 43 45 83 93 123 147 163 209 203 282
4 4 41 45 78 96 115 162 152 258 189 432 4 4 44 47 84 E 124 148 164 211 204 284
5 5 42 46 79 98 116 165 153 261 190 439 5 5 45 48 85 95 125 150 165 212 205 287
6 6 43 48 80 99 117 167 154 265 191 447 6 6 46 49 86 97 126 151 166 214 206 289
7 7 44 49 81 101 118 169 155 268 192 455 7 7 47 50 87 98 127 152 167 216 207 291
8 8 45 50 82 102 119 171 156 271 193 463 8 8 48 51 88 99 128 154 168 217 208 293
9 9 46 51 83 104 120 173 157 275 194 471 9 9 49 52 89 100 129 155 169 219 209 295
10 10 47 53 84 106 121 176 158 278 195 480 10 10 50 53 90 102 130 157 170 221 210 297
11 11 48 54 85 107 122 178 159 282 196 489 11 11 51 54 91 103 131 158 171 223 211 299
12 12 49 55 86 109 123 180 160 286 197 499 12 12 52 56 92 104 132 160 172 224 212 301
13 13 50 57 87 110 124 182 161 289 198 508 13 13 53 57 93 106 133 161 173 226 213 303
14 14 51 58 88 112 125 185 162 293 199 519 14 14 54 58 94 107 134 163 174 228 214 305
15 15 52 59 89 114 126 187 163 297 200 530 15 15 55 59 95 108 135 164 175 230 215 307
16 17 53 60 90 116 127 189 164 301 201 542 16 16 56 60 96 110 136 166 176 231 216 310
17 18 54 62 91 117 128 192 165 305 202 554 17 17 57 61 97 111 137 167 177 233 217 312
18 19 55 63 92 119 129 194 166 309 203 567 18 18 58 63 98 112 138 169 178 235 218 314
19 20 56 64 93 121 130 196 167 313 204 580 19 19 59 64 99 114 139 170 179 237 219 316
20 21 57 66 94 122 131 199 168 317 205 595 20 20 60 65 100 115 140 172 180 239 220 318
21 22 58 67 95 124 132 201 169 322 206 611 21 22 61 66 101 116 141 173 181 240 221 321
22 23 59 69 96 126 133 204 170 326 207 627 22 23 62 67 102 118 142 175 182 242 222 323
23 24 60 70 97 128 134 206 171 331 208 646 23 24 63 68 103 119 143 177 183 244 223 325
24 25 61 71 98 130 135 209 172 335 209 666 24 25 64 70 104 120 144 178 184 246 224 327
25 26 62 73 99 131 136 212 173 340 210 687 25 26 65 71 105 122 145 180 185 248 225 330
26 28 63 74 100 133 137 214 174 344 211 712 26 27 66 72 106 123 146 181 186 250 226 332
27 29 64 76 101 135 138 217 175 349 212 739 27 28 67 73 107 124 147 183 187 251 227 334
28 30 65 77 102 137 139 220 176 354 213 770 28 29 68 74 108 126 148 184 188 253 228 336
29 31 66 78 103 139 140 222 177 359 214 807 29 30 69 76 109 127 149 186 189 255 229 339
30 32 67 80 104 141 141 225 178 365 215 851 30 31 70 77 110 128 150 188 190 257 230 341
31 33 68 81 105 142 142 228 179 370 216 905 31 32 71 78 111 130 151 189 191 259 231 343
32 34 69 83 106 144 143 231 180 375 217 978 32 33 72 79 112 131 152 191 192 261 232 346
33 36 70 84 107 146 144 234 181 381 218 1088 33 34 73 80 113 133 153 192 193 263 233 348
34 37 71 86 108 148 145 237 182 387 219 1307 34 35 74 82 114 134 154 194 194 265 234 351
35 38 72 87 109 150 146 240 183 393 35 37 75 83 115 135 155 196 195 267 235 353
36 39 73 88 110 152 147 243 184 399 36 38 76 84 116 137 156 197 196 269 236 355
37 40 74 90 111 154 148 246 185 405 37 39 77 85 117 138 157 199 197 271 237 358
38 40 78 87 118 140 158 201 198 272 238 360
39 41 79 88 119 141 159 202 199 274 239 363
40 42 80 89 120 142 160 204 200 276 240 365
55mm contact plates - 90mm Petri Plates - 219 holes 1,00 mm 55mm contact plates - 90mm Petri Plates - 401 holes 0,75 mm
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Accessories
Description Cat. No.
Spare parts
Aluminium aspirating head for contact plates, Ø 55 mm 710-0892
Aluminium aspirating head for Petri dishes, Ø 90 mm 710-0886
SAS Super universal battery charger 710-0993
Remote control 710-0962
Soft carrying case 710-0896
Other aspirating heads for contact plates, Ø 55 mm
Stainless steel aspirating head for contact plates, Ø 55 mm 710-0880
Sterile daily head for contact plates, Ø 55 mm 710-0890
Other aspirating heads for Petri dishes, Ø 90 mm
Stainless steel aspirating head for Petri dishes, Ø 90 mm 710-0878
Sterile daily head for Petri dishes, Ø 90 mm 710-0891
Other accessories
Aluminium carrying case 710-0875
Bio-Transport autoclavable carrying case 113-8185
Handle for Bio-Transport carrying case 113-8186
Floor tripod 710-0889
SAS-Holder table and wall stainless steel 710-0963
Adaptor* to convert contact plate model to accept 90 mm Petri dishes 710-0882
SAS stainless steel Petri head + adaptor 710-0877
SAS aluminium Petri head + adaptor 710-0879
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Troubleshooting
Review the information in the table below to troubleshoot operating problems.
Problem Cause Solution
Unit does not start Battery is low Recharge battery
Battery charger is not working Check battery charger and replace if
required (Cat. No. 710-0993)
Battery is too old Check battery and replace if required
Battery discharges after few minutes
working
Battery is low Recharge battery
Battery charger is not working Check battery charger and replace if
required (Cat. No. 710-0973)
Battery is too old Check battery and eventually replace it
“LOW BATTERY” message Battery is low Recharge battery
Infrared remote switch doesn’t switch
on the unit
Remote control battery is low Replace infrared remote battery
Microbiological media is dehydrated
after sampling
Media is corrupted Check expiration date of media and if
the
agar was not dehydrated before
sampling
Sampling time is too long Shorten the sampling time
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Technical service
Web resources
Visit the VWR website at vwr.com for:
• Complete technical service contact information
• Access to the VWR online catalogue and information about accessories and related products
• Additional product information and special offers
Contact us for information or technical assistance contact your local VWR representative or visit vwr.com.
Warranty
VWR International warrants that this product will be free from defects in material and workmanship for a period
of two (2) years from date of delivery. If a defect is present, VWR will, at its option and cost, repair, replace, or
refund the purchase price of this product to the customer, provided it is returned during the warranty period. This
warranty does not apply if the product has been damaged by accident, abuse, misuse, or misapplication, or from
ordinary wear and tear. If the required maintenance and inspection services are not performed according to the
manuals and any local regulations, such warranty turns invalid, except to the extent, the defect of the product is not
due to non performance.
Items being returned must be insured by the customer against possible damage or loss. This warranty shall be
limited to the aforementioned remedies. IT IS EXPRESSLY AGREED THAT THIS WARRANTY WILL BE IN LIEU
OF ALL WARRANTIES OF FITNESS AND IN LIEU OF THE WARRANTY OF MERCHANTABILITY.
Compliance with local laws and regulations
The customer is responsible for applying for and obtaining the necessary regulatory approvals or other
authorisations necessary to run or use the product in its local environment. VWR will not be held liable for any
related omission or for not obtaining the required approval or authorisation, unless any refusal is due to a defect of
the product.
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