Amersham Pharmacia Biotech EPS 601 User manual

Electrophoresis Power Supply
EPS 601
User Manual
Manuel d’Utilization
Bedienungsanleitung
Manual del usuario
Manuale dell’operatore
18-1130-19
Edition AB
18-1130-19 AA EPS 601 omslag 99-02-09 14.10 Sidan 1

Program
HV on
Electrophoresis Power Supply - EPS 601
STOP
run exit enter
more set
pause
continue
Voltage 100-120/220-240 VAC
Frequency 50/60 Hz
Power 140 W 2.5AMax
100-120 220-240
MAINS
Disconnect before servicing
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Fig. 1. The front panel of the EPS 601.
Figure 1. Le panneau avant de l’EPS 601.
Abb. 1. Die Fronttafel des EPS 601.
Figura 1. Panel frontal de la EPS 601.
Figura 1. Pannello anteriore dell’EPS 601.
Fig. 2. The rear panel of the EPS 601.
Figure 2. Le panneau arrière de l’EPS 601.
Abb. 2. Die Rückwand des EPS 601.
Figura 2. Panel posterior de la EPS 601.
Figura 2. Pannello posteriore dell’EPS 601.
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1. Introduction. . . . . . . . . . . . . . . . . . . . . . 2
2. Safety information . . . . . . . . . . . . . . . . . 2
2.1 Safety precautions . . . . . . . . . . . . . . 2
2.2 In-built safety features . . . . . . . . . . . 3
3. Unpacking and installation. . . . . . . . . . . 3
4. Technical description . . . . . . . . . . . . . . . 4
4.1 Front panel . . . . . . . . . . . . . . . . . . . 4
4.1.1 Display . . . . . . . . . . . . . . . . . . 4
4.1.2 Keyboard . . . . . . . . . . . . . . . . 5
4.1.3 Output sockets . . . . . . . . . . . . 5
4.2 Rear panel . . . . . . . . . . . . . . . . . . . . 5
5. Operation. . . . . . . . . . . . . . . . . . . . . . . . 6
5.1 Overview. . . . . . . . . . . . . . . . . . . . . 6
5.2 Programming. . . . . . . . . . . . . . . . . . 6
5.3 Running a program . . . . . . . . . . . . . 8
5.4 Optional programming . . . . . . . . . . 9
5.5 Choosing run parameters. . . . . . . . 10
5.6 Short instructions. . . . . . . . . . . . . . 11
6. Maintenance . . . . . . . . . . . . . . . . . . . . 12
7. Trouble shooting . . . . . . . . . . . . . . . . . 12
8. Technical specifications . . . . . . . . . . . . 13
9. Ordering information. . . . . . . . . . . . . . 14
Contents
EPS 601 - User Manual
Please read this entire manual to
fully understand the safe use of
EPS 601
WARNING!
The Warning sign
highlights an instruction
that must be strictly
followed in order to
avoid personal injury. Be sure not to
proceeed until the instructions are
clearly understood and all stated
conditions are met.
Declaration of conformity
Safety Standards
This product complies with the
requirement of the Low Voltage
Directive (LVD) 73/23/EEC through
the harmonized standard EN 61010-
1, 1993+ A1, 1992.
Important user information
EMC Standards
This product complies with the
requirement of the EMC Directive
89/336/EEC through the harmonized
standards EN 50081-1 (emission)
and EN 50082-1 (immunity).
The CE symbol, and corresponding
declaration of conformity, is valid
for the instrument when it is.
– used as a “stand alone” unit or
– connected to other CE marked
Amersham Pharmacia Biotech
instruments, or
– connected to other products
recommended or described in
this manual and
– is used in the same state as it was
delivered from Amersham
Pharmacia Biotech except for
alterations described in this
manual.
Terms and Conditions of Sale
All goods and services are sold
subject to the terms and conditions
of sale of the company within the
Amersham Pharmacia Biotech group
which supplies them. A copy of these
terms and conditions is available on
request.
Should you have any comments on
this product, we will be pleased to
receive them at:
Amersham Pharmacia Biotech Inc.
654 Minnesota Street
San Francisco, CA 94107 USA
Trademarks
Amersham is a trademark of
Nycomed Amersham plc.
Pharmacia and Drop Design are
trademarks of Pharmacia & Upjohn
Inc.
© Copyright 1998 Amersham
Pharmacia Biotech AB - All rights
reserved
Office Addresses
Amersham Pharmacia Biotech AB
SE-751 84 Uppsala
Sweden
Amersham Pharmacia Biotech UK
Limited
Amersham Place Little Chalfont
Buckinghamshire
England HP7 9NA
Amersham Pharmacia Biotech Inc.
800 Centennial Avenue
P.O. Box 1327
Piscataway N.J. 08855-1327
USA
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EPS 601 - User Manual
1. Introduction
The Amersham Pharmacia Biotech Electrophoresis Power Supply EPS 601 is a high quality, high
precision and safe power supply for electrophoresis applications that require a maximum of 600 volts
and 400 mA.
EPS 601 is primarily designed for the following techniques:
1. SDS-PAGE (Polyacrylamide Gel Electrophoresis)
2. Native PAGE
3. Agarose electrophoresis
4. Electroblotting
5. DNA pulsed field electrophoresis
6. DNA fragment separation
7. DNA submarine electrophoresis
Electrophoresis separations can be controlled by voltage, current or power. The EPS 601 automatically
switches over the controlling parameter according to programmed limits and conductivity variations in
the system.
Two electrophoresis units can be connected to the EPS 601 and run with the same programmed method
at one time. Three programs can be saved.
2. Safety information
2.1 Safety precaucions
Extreme caution should be exercised in the operation of this instrument as it can develop sufficient
voltage and current to produce a lethal shock.
To avoid any risk of injury, the instrument should only be operated by properly trained personnel and
always in accordance with the instructions provided.
Read this entire manual before using this power supply.
1. This instrument is designed for indoor use only.
2. The instrument must always be used with the protective earth lead of the power cord correctly
grounded to earth at the mains outlet.
3. To permit sufficient cooling, ensure that the vents in the rear and sides of the instrument are not
covered.
4. Do not operate the instrument in extreme humidity (above 95%). Avoid condensation by letting the
unit equilibrate to ambient temperature when taking the power supply from a colder to a warmer
environment.
5. Keep the instrument as dry and clean as possible. Wipe regularly with a soft damp cloth. Let the
power supply dry completely before use. If wetted, unplug the power supply until the instrument is
dry.
6. Use only undamaged electrical wire and equipment specified for the voltages you will use. High
voltages wires must meet the requirements of the IEC 1010-2-031:1993 electrical standard. Any
electrophoresis equipment connected to the power supply should meet the requirements of the IEC
1010-1:1993 electrical standard.
7. Note that the output is connected to the chassis/reference earth.
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EPS 601 - User Manual
2.2 Built-in safety features
The EPS 601 has been tested and complies with the IEC 61010-1 (EN 61010-1) electrical safety
standard. The power supply also has several built-in safety functions. Error messages are also shown on
the display (see 7. Trouble shooting):
1. Functional earth leakage.
Should the power supply be connected to an electrophoresis unit that has a leakage path to earth, the
EPS 601 will detect this fault and the high voltage is turned off.
2. Start current check.
To ensure that an electrophoresis unit is connected correctly, the power supply checks that the resistance
is not higher than a specified limit at a low safety voltage (<40V). If this resistance is too high, the
voltage is turned off. Too high a resistance can also be caused by using buffers with extremely low
conductivity. The high voltage is also turned off in this case and an error message is shown. This
function can be disabled to perform certain applications (see 5.4 Optional programming).
3. Sudden load change detection.
This function prevents accidents under running conditions due to a break in the electrical circuit such as
a bad connection to the electrophoresis unit. The high voltage is turned off in such an event.
Error messages for sudden fails are also shown on the display.
3. Unpacking and installation
Unpacking
Check the contents against the packing list supplied. Inspect for any damage that may have occurred
during transit. Report any damage immediately to your local Amersham Pharmacia Biotech
representative and to the transport company concerned.
Mains connection
Select the appropriate voltage range, 100-120 or 220-240 V. See Fig. 2 inside front cover.
Warning! If the power supply is connected to 220-240 V with the range set to 100-120 V, the
instrument can be severely damaged.
Select the appropriate mains cable and connect one end to the mains socket on the EPS 601 power
supply (see Fig. 2, inside front cover) and the other end to an AC grounded outlet.
Connection of the electrophoresis unit(s)
Connect the leads from the electrophoresis unit (red to red, and black or blue to blue), see Fig. 1 inside
front cover. The red lead is the positive and black or blue is the negative.
Warning! Use only undamaged electrical wire and equipment approved for the voltage you will use.
Two electrophoresis units can be run simultaneously with the same program. Please remember to
double the limiting current and power if two electrophoresis units are run at the same time. The
voltage will be the same regardless of the number of units.
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