Analytic Jena PCR UV3 HEPA Guide

PCR UV3HEPA
Cabinets and Workstations
Installation and User Instructions

Analytik Jena AG
Customer Services
Konrad-Zuse-Str. 1
07745 Jena
Germany
General information about Analytik Jena AG
On the internet: http://www.analytik-jena.de
Copyrights and Trademarks
Makrolon is a registered trademark of Bayer Corp.
Documentation number: 37-3700-012-23
Edition – September 2013 (Rev STD)
Implementation of the Technical Documentation:
Analytik Jena AG
This publication describes the state of this product at the time of publishing. It need not necessarily
agree with future versions of the product.
Modifications reserved!
© Copyright 2013 Analytik Jena AG

Contents
PCR UV3 HEPA Systems Edition 09/2013 1
Contents
1Basic Information ........................................................................................................ 3
1.1 User manual notes .............................................................................................. 3
1.2 Intended use ........................................................................................................ 3
1.3 Warranty and liability ........................................................................................... 4
2Technical data.............................................................................................................. 5
3Safety instructions ...................................................................................................... 7
3.1 General notes ...................................................................................................... 7
3.2 Symbols and signal words used.......................................................................... 7
3.3 Safety markings at the PCR UV3HEPA Systems ............................................... 8
3.4 Technical condition.............................................................................................. 8
3.5 Requirements for the operating personnel .......................................................... 8
3.6 Safety instructions – transport and assembly ..................................................... 9
3.7 Safety instructions – operation ............................................................................ 9
3.8 Handling of auxiliary and operating materials ................................................... 10
3.9 Safety instructions – maintenance .................................................................... 10
3.10 Behavior during emergencies............................................................................ 11
4Technical description ................................................................................................ 12
5Operational ratings.................................................................................................... 13
6Assembling the PCR UV3HEPA Systems ............................................................... 14
7Operating the PCR UV3HEPA Systems................................................................... 19
7.1 Use of PCR equipment inside the Cabinet or Workstation ............................... 19
7.2 Operating the HEPA/UV System....................................................................... 20
7.3 Operating the UV/Air Circulator......................................................................... 20
7.4 Operating the overhead UV and fluorescent white light.................................... 21
7.5 Measuring the UV tube intensity ....................................................................... 22
7.6 Care and cleaning ............................................................................................. 24
8Maintenance, replacement parts/accessories ........................................................ 25
8.1 Replacement parts and accessories ................................................................. 25
8.2 Replacing tubes in the chamber........................................................................ 26
8.3 Replacing the fuses ........................................................................................... 27
8.4 Replacing the tube in the UV/Air Circulator....................................................... 27
8.5 Replacing the filters ........................................................................................... 28
8.6Replacing UV tube in the filter system .............................................................. 30
9Disposal ...................................................................................................................... 30

Figures
2 Edition 09/2013 PCR UV3 HEPA Systems
Figures
Figure 1 Attaching the outer back panel .......................................................................... 14
Figure 2 Attaching the inner back panel .......................................................................... 15
Figure 3 Assembling the Makrolon panels....................................................................... 16
Figure 4 Connecting the bottom and top assembly wires together ................................. 17
Figure 5 Positioning the top assembly............................................................................. 17
Figure 6 Assembling the door .......................................................................................... 18
Figure 7 Specific power plugs.......................................................................................... 19
Figure 8 Decontaminated airflow into and out of the chamber........................................ 20
Figure 9 Operating the UV and fluorescent white lights .................................................. 21
Figure 10 PCR Table ......................................................................................................... 26
Figure 11Light tubes in PCR UV3HEPA Cabinet and Workstation.................................. 26
Figure 12 Replacing the fuses ........................................................................................... 27
Figure 13 Replacing the tube in the UV/Air Circulator....................................................... 27
Figure 14 The filter assembly with the Pre-filter (1) ........................................................... 28
Figure 15 The filter assembly with the Carbon filter .......................................................... 28
Figure 16 The filter assembly after the carbon filter has been removed ........................... 29
Figure 17 The filter assembly with the HEPA filter (1)....................................................... 29

Basic Information
PCR UV3 HEPA Systems Edition 09/2013 3
1 Basic Information
1.1 User manual notes
The PCR UV3HEPA Systems are intended for operation by qualified specialist personnel
observing this user manual.
The user manual informs about the design and function of the PCR UV3HEPA Systems and
provides the necessary know-how for the safe handling of the device and its components to
personnel familiar with analysis. The user manual further includes notes on the maintenance
and service of the equipment.
User manual conventions
Instructions for action which occur in chronological order are numbered and combined into
action units and furnished with the corresponding results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.
Safety notes are indicated by pictographs and signal words. The type and source of the
danger are stated together with notes on preventing the danger. The meaning of the picto-
graphs and signal words used is explained in the chapter "Safety instructions".
1.2 Intended use
The PCR UV3HEPA Systems create an ideal environment for preparing PCR and other
samples by reducing the chance of contamination with the filtered air treated by the
HEPA/UV system which blows into the PCR chamber. The built-in high intensity UV provides
a source for inactivation of DNA between experiments.
The operational safety of the PCR UV3HEPA Systems is only ensured during proper use
according to the information in this user manual. The intended use also includes the adher-
ence to the installation conditions prescribed by Analytik Jena AG.
The device must only be commissioned, operated and serviced by trained personnel in-
structed in technical safety.
Non-observance of the safety notes given for the use of the PCR UV3HEPA Systems is
regarded as departure from intended use. Follow the safety notes given in particular labels
on the device, in section 3 “Safety instructions” and in the description of the required steps of
operation.

Basic Information
4 Edition 09/2013 PCR UV3 HEPA Systems
1.3 Warranty and liability
The warranty duration and liability comply with the legal requirements and the provisions in
the general terms and conditions of Analytik Jena AG. Tubes and filters are warranted for 90
days. Crazing of the Makrolon panels may occur within the warranty period and is regarded
as normal wear and tear not covered by the warranty.
Deviations from the intended use described in this user manual result in limitations of war-
ranty and liability during a damage event. Damage to wearing parts is not included in the
warranty.
Warranty and liability claims are excluded for personal injury and property damage due to
one or several of the following causes:
use of the PCR UV3HEPA System other than intended
improper commissioning, operation and service of the device
modifications of the equipment without prior consultation with Analytik Jena AG
unauthorized intervention in the equipment
operation of the device with faulty safety equipment or improperly fitted safety and
protection equipment
inadequate monitoring of the equipment components subject to wear
use of other than original spare parts, wearing parts or consumables
improper repairs
faults due to the non-observance of this user manual

Technical data
PCR UV3 HEPA Systems Edition 09/2013 5
2 Technical data
Standard PCR UV3HEPA Models
PCR UV3HEPA Cabinet PCR UV3HEPA Workstation
849-00002-2 PCR UV³ HEPA Cabinet,
230 V (UK plug)
849-00002-3 PCR UV³ HEPA Cabinet,
230 V (Euro plug)
849-00002-4 PCR UV³ HEPA Cabinet,
115 V (US plug)
849-00002-5 PCR UV³ HEPA Cabinet,
100 V (US plug)
849-00006-2 PCR UV³ HEPA Workstation,
230 V (UK plug)
849-00006-3 PCR UV³ HEPA Workstation,
230 V (Euro plug)
849-00006-4 PCR UV³ HEPA Workstation,
115 V (US plug)
849-00006-5 PCR UV³ HEPA Workstation,
100 V (US plug)
Light Sources
254 nm 8 Watt UV:
– Filter area
– Chamber
– UV/Air Circulator
White 8 Watt:
– Chamber
254 nm:
– Filter area 8 Watt
– Chamber 25 Watt
– UV/Air Circulator 8 Watt
White 8 Watt:
– Chamber
Three stage filters
– Pre-filter
– Carbon filter
– HEPA filter
– Pre-filter
– Carbon filter
– HEPA filter
Features include
Two power outlets
Two small shelves
UV Timer
Antimicrobial coated stainless steel (work
surface) & aluminum
Makrolon panels block below 400 nm
Four power outlets
Two shelves
UV Timer
Antimicrobial coated stainless steel (work
surface) & aluminum
Makrolon panels block below 400 nm
Dimensions
Exterior (H x W x D): 32.5 x 21.4 x 24 in.
826 x 544 x 610 mm
Exterior (H x W x D): 32.5 x 29 x 24 in.
826 x 737 x 610 mm
Interior (W x D): 19.7 x 21.4 in.
500 x 544 mm
Interior (W x D): 27.8 x 21.4 in.
706 x 544 mm

Technical data
6 Edition 09/2013 PCR UV3 HEPA Systems
UV / HEPA
Three stage filter Pre-filter, Carbon filter and HEPA filter
UV Source One 8 Watt 254 nm shortwave UV tube
Chamber Light Sources
Cabinet
UV Source – Chamber
UV Air Circulator
White Light
Four 8 Watt 254 nm shortwave UV tubes
One 8 Watt 254 nm shortwave UV tube
Two 8 Watt white tubes
Workstation
UV Source – Chamber
UV Air Circulator
White Light
Two 25 Watt 254 nm shortwave UV tubes
One 8 Watt 254 nm shortwave UV tube
Two 8 Watt white tubes
Power Outlets
Design Built-in outlets for operating instruments in-
side the chamber:
Four in the Workstation, two in the Cabinet
Shelves
Construction Two – formed antimicrobial coated aluminum
Dimensions (Cabinet) (W x D) 13 x 4.2 in. (330 x 107 mm)
Dimensions (Workstation) (W x D) 8.75 x 4 in. (222 x 102 mm)
System Design
Interior Antimicrobial coated stainless steel (horizon-
tal work surface) and aluminum
Exterior Aluminum powder coated
Door and side panels single panel, Makrolon blocks wavelengths
below 400 nm
Electrical
115 V 60 Hz
230 V 50 Hz
100 V 50/60 Hz

Safety instructions
PCR UV3 HEPA Systems Edition 09/2013 7
3 Safety instructions
3.1 General notes
For your own safety and to ensure error-free and safe operation of the PCR UV3HEPA Sys-
tems, please read this chapter carefully before commissioning.
Observe all safety notes listed in this user manual.
Besides the safety instructions in this user manual and the local safety regulations that apply
to the operation of the device the general applicable regulations regarding accident preven-
tion, occupational health and safety and environmental protection have to be observed and
complied with.
References to potential dangers do not replace the work protection regulations which must
be observed.
IMPORTANT
Specific hazards that might arise during PCR experiments are pointed out in the separate
user manual.
Always take note of this information together with the notes in this user manual!
3.2 Symbols and signal words used
The user manual uses the following symbols and signal words to indicate hazards or instruc-
tions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation
If it is not prevented death or most serious injuries (incapacitation) can result.
CAUTION
Indicates a potentially hazardous situation
If it is not prevented light or minor injuries and material damage can result.
ATTENTION
Indicates a potentially hazardous situation
Unless avoided, the product or items in its vicinity may be damaged.
IMPORTANT
Indicates application hints and other especially useful information without any resulting haz-
ardous or damaging situations

Safety instructions
8 Edition 09/2013 PCR UV3 HEPA Systems
3.3 Safety markings at the PCR UV3HEPA Systems
Safety symbols have been attached to the PCR UV3HEPA Systems whose content must
always be observed. Damaged or missing safety symbols can cause incorrect actions lead-
ing to personal injury or material damage! The safety symbols must not be removed! Dam-
aged safety symbols must be replaced without delay!
The following safety symbols have been attached to the PCR UV3HEPA Systems:
General warning
Caution: Radiation, ultra-
violet
3.4 Technical condition
The PCR UV3HEPA Systems correspond in their design and construction to the current
state of the art technology. Unauthorized modifications or changes, especially such that af-
fect the safety of the staff and the environment, are generally not allowed.
Observe the following notes:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating
personnel to the operator without delay.
3.5 Requirements for the operating personnel
Observe the following notes:
The device must only be commissioned, operated and serviced by trained person-
nel instructed in technical safety.
The operation or servicing of the device by minors or individuals under the influ-
ence of alcohol, drugs or medication is not permitted.
It must be ensured that only authorized personnel works at the device.
The operating personnel must be familiar with the dangers arising from samples to
be used. The appropriate protective equipment must be used.
The unit includes shortwave UV, which is a powerful source of UV radiation that
will cause damage to unprotected eyes and skin. Before operating any unit, ensure
all personnel in the area are properly protected and that instructions for use of this
equipment are followed.