Analytic Jena PCR UV3 HEPA Guide

PCR UV3HEPA
Cabinets and Workstations
Installation and User Instructions

Analytik Jena AG
Customer Services
Konrad-Zuse-Str. 1
07745 Jena
Germany
General information about Analytik Jena AG
On the internet: http://www.analytik-jena.de
Copyrights and Trademarks
Makrolon is a registered trademark of Bayer Corp.
Documentation number: 37-3700-012-23
Edition – September 2013 (Rev STD)
Implementation of the Technical Documentation:
Analytik Jena AG
This publication describes the state of this product at the time of publishing. It need not necessarily
agree with future versions of the product.
Modifications reserved!
© Copyright 2013 Analytik Jena AG

Contents
PCR UV3 HEPA Systems Edition 09/2013 1
Contents
1Basic Information ........................................................................................................ 3
1.1 User manual notes .............................................................................................. 3
1.2 Intended use ........................................................................................................ 3
1.3 Warranty and liability ........................................................................................... 4
2Technical data.............................................................................................................. 5
3Safety instructions ...................................................................................................... 7
3.1 General notes ...................................................................................................... 7
3.2 Symbols and signal words used.......................................................................... 7
3.3 Safety markings at the PCR UV3HEPA Systems ............................................... 8
3.4 Technical condition.............................................................................................. 8
3.5 Requirements for the operating personnel .......................................................... 8
3.6 Safety instructions – transport and assembly ..................................................... 9
3.7 Safety instructions – operation ............................................................................ 9
3.8 Handling of auxiliary and operating materials ................................................... 10
3.9 Safety instructions – maintenance .................................................................... 10
3.10 Behavior during emergencies............................................................................ 11
4Technical description ................................................................................................ 12
5Operational ratings.................................................................................................... 13
6Assembling the PCR UV3HEPA Systems ............................................................... 14
7Operating the PCR UV3HEPA Systems................................................................... 19
7.1 Use of PCR equipment inside the Cabinet or Workstation ............................... 19
7.2 Operating the HEPA/UV System....................................................................... 20
7.3 Operating the UV/Air Circulator......................................................................... 20
7.4 Operating the overhead UV and fluorescent white light.................................... 21
7.5 Measuring the UV tube intensity ....................................................................... 22
7.6 Care and cleaning ............................................................................................. 24
8Maintenance, replacement parts/accessories ........................................................ 25
8.1 Replacement parts and accessories ................................................................. 25
8.2 Replacing tubes in the chamber........................................................................ 26
8.3 Replacing the fuses ........................................................................................... 27
8.4 Replacing the tube in the UV/Air Circulator....................................................... 27
8.5 Replacing the filters ........................................................................................... 28
8.6Replacing UV tube in the filter system .............................................................. 30
9Disposal ...................................................................................................................... 30

Figures
2 Edition 09/2013 PCR UV3 HEPA Systems
Figures
Figure 1 Attaching the outer back panel .......................................................................... 14
Figure 2 Attaching the inner back panel .......................................................................... 15
Figure 3 Assembling the Makrolon panels....................................................................... 16
Figure 4 Connecting the bottom and top assembly wires together ................................. 17
Figure 5 Positioning the top assembly............................................................................. 17
Figure 6 Assembling the door .......................................................................................... 18
Figure 7 Specific power plugs.......................................................................................... 19
Figure 8 Decontaminated airflow into and out of the chamber........................................ 20
Figure 9 Operating the UV and fluorescent white lights .................................................. 21
Figure 10 PCR Table ......................................................................................................... 26
Figure 11Light tubes in PCR UV3HEPA Cabinet and Workstation.................................. 26
Figure 12 Replacing the fuses ........................................................................................... 27
Figure 13 Replacing the tube in the UV/Air Circulator....................................................... 27
Figure 14 The filter assembly with the Pre-filter (1) ........................................................... 28
Figure 15 The filter assembly with the Carbon filter .......................................................... 28
Figure 16 The filter assembly after the carbon filter has been removed ........................... 29
Figure 17 The filter assembly with the HEPA filter (1)....................................................... 29

Basic Information
PCR UV3 HEPA Systems Edition 09/2013 3
1 Basic Information
1.1 User manual notes
The PCR UV3HEPA Systems are intended for operation by qualified specialist personnel
observing this user manual.
The user manual informs about the design and function of the PCR UV3HEPA Systems and
provides the necessary know-how for the safe handling of the device and its components to
personnel familiar with analysis. The user manual further includes notes on the maintenance
and service of the equipment.
User manual conventions
Instructions for action which occur in chronological order are numbered and combined into
action units and furnished with the corresponding results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.
Safety notes are indicated by pictographs and signal words. The type and source of the
danger are stated together with notes on preventing the danger. The meaning of the picto-
graphs and signal words used is explained in the chapter "Safety instructions".
1.2 Intended use
The PCR UV3HEPA Systems create an ideal environment for preparing PCR and other
samples by reducing the chance of contamination with the filtered air treated by the
HEPA/UV system which blows into the PCR chamber. The built-in high intensity UV provides
a source for inactivation of DNA between experiments.
The operational safety of the PCR UV3HEPA Systems is only ensured during proper use
according to the information in this user manual. The intended use also includes the adher-
ence to the installation conditions prescribed by Analytik Jena AG.
The device must only be commissioned, operated and serviced by trained personnel in-
structed in technical safety.
Non-observance of the safety notes given for the use of the PCR UV3HEPA Systems is
regarded as departure from intended use. Follow the safety notes given in particular labels
on the device, in section 3 “Safety instructions” and in the description of the required steps of
operation.

Basic Information
4 Edition 09/2013 PCR UV3 HEPA Systems
1.3 Warranty and liability
The warranty duration and liability comply with the legal requirements and the provisions in
the general terms and conditions of Analytik Jena AG. Tubes and filters are warranted for 90
days. Crazing of the Makrolon panels may occur within the warranty period and is regarded
as normal wear and tear not covered by the warranty.
Deviations from the intended use described in this user manual result in limitations of war-
ranty and liability during a damage event. Damage to wearing parts is not included in the
warranty.
Warranty and liability claims are excluded for personal injury and property damage due to
one or several of the following causes:
use of the PCR UV3HEPA System other than intended
improper commissioning, operation and service of the device
modifications of the equipment without prior consultation with Analytik Jena AG
unauthorized intervention in the equipment
operation of the device with faulty safety equipment or improperly fitted safety and
protection equipment
inadequate monitoring of the equipment components subject to wear
use of other than original spare parts, wearing parts or consumables
improper repairs
faults due to the non-observance of this user manual

Technical data
PCR UV3 HEPA Systems Edition 09/2013 5
2 Technical data
Standard PCR UV3HEPA Models
PCR UV3HEPA Cabinet PCR UV3HEPA Workstation
849-00002-2 PCR UV³ HEPA Cabinet,
230 V (UK plug)
849-00002-3 PCR UV³ HEPA Cabinet,
230 V (Euro plug)
849-00002-4 PCR UV³ HEPA Cabinet,
115 V (US plug)
849-00002-5 PCR UV³ HEPA Cabinet,
100 V (US plug)
849-00006-2 PCR UV³ HEPA Workstation,
230 V (UK plug)
849-00006-3 PCR UV³ HEPA Workstation,
230 V (Euro plug)
849-00006-4 PCR UV³ HEPA Workstation,
115 V (US plug)
849-00006-5 PCR UV³ HEPA Workstation,
100 V (US plug)
Light Sources
254 nm 8 Watt UV:
– Filter area
– Chamber
– UV/Air Circulator
White 8 Watt:
– Chamber
254 nm:
– Filter area 8 Watt
– Chamber 25 Watt
– UV/Air Circulator 8 Watt
White 8 Watt:
– Chamber
Three stage filters
– Pre-filter
– Carbon filter
– HEPA filter
– Pre-filter
– Carbon filter
– HEPA filter
Features include
Two power outlets
Two small shelves
UV Timer
Antimicrobial coated stainless steel (work
surface) & aluminum
Makrolon panels block below 400 nm
Four power outlets
Two shelves
UV Timer
Antimicrobial coated stainless steel (work
surface) & aluminum
Makrolon panels block below 400 nm
Dimensions
Exterior (H x W x D): 32.5 x 21.4 x 24 in.
826 x 544 x 610 mm
Exterior (H x W x D): 32.5 x 29 x 24 in.
826 x 737 x 610 mm
Interior (W x D): 19.7 x 21.4 in.
500 x 544 mm
Interior (W x D): 27.8 x 21.4 in.
706 x 544 mm

Technical data
6 Edition 09/2013 PCR UV3 HEPA Systems
UV / HEPA
Three stage filter Pre-filter, Carbon filter and HEPA filter
UV Source One 8 Watt 254 nm shortwave UV tube
Chamber Light Sources
Cabinet
UV Source – Chamber
UV Air Circulator
White Light
Four 8 Watt 254 nm shortwave UV tubes
One 8 Watt 254 nm shortwave UV tube
Two 8 Watt white tubes
Workstation
UV Source – Chamber
UV Air Circulator
White Light
Two 25 Watt 254 nm shortwave UV tubes
One 8 Watt 254 nm shortwave UV tube
Two 8 Watt white tubes
Power Outlets
Design Built-in outlets for operating instruments in-
side the chamber:
Four in the Workstation, two in the Cabinet
Shelves
Construction Two – formed antimicrobial coated aluminum
Dimensions (Cabinet) (W x D) 13 x 4.2 in. (330 x 107 mm)
Dimensions (Workstation) (W x D) 8.75 x 4 in. (222 x 102 mm)
System Design
Interior Antimicrobial coated stainless steel (horizon-
tal work surface) and aluminum
Exterior Aluminum powder coated
Door and side panels single panel, Makrolon blocks wavelengths
below 400 nm
Electrical
115 V 60 Hz
230 V 50 Hz
100 V 50/60 Hz

Safety instructions
PCR UV3 HEPA Systems Edition 09/2013 7
3 Safety instructions
3.1 General notes
For your own safety and to ensure error-free and safe operation of the PCR UV3HEPA Sys-
tems, please read this chapter carefully before commissioning.
Observe all safety notes listed in this user manual.
Besides the safety instructions in this user manual and the local safety regulations that apply
to the operation of the device the general applicable regulations regarding accident preven-
tion, occupational health and safety and environmental protection have to be observed and
complied with.
References to potential dangers do not replace the work protection regulations which must
be observed.
IMPORTANT
Specific hazards that might arise during PCR experiments are pointed out in the separate
user manual.
Always take note of this information together with the notes in this user manual!
3.2 Symbols and signal words used
The user manual uses the following symbols and signal words to indicate hazards or instruc-
tions. The safety instructions are always placed before an action.
WARNING
Indicates a potentially hazardous situation
If it is not prevented death or most serious injuries (incapacitation) can result.
CAUTION
Indicates a potentially hazardous situation
If it is not prevented light or minor injuries and material damage can result.
ATTENTION
Indicates a potentially hazardous situation
Unless avoided, the product or items in its vicinity may be damaged.
IMPORTANT
Indicates application hints and other especially useful information without any resulting haz-
ardous or damaging situations

Safety instructions
8 Edition 09/2013 PCR UV3 HEPA Systems
3.3 Safety markings at the PCR UV3HEPA Systems
Safety symbols have been attached to the PCR UV3HEPA Systems whose content must
always be observed. Damaged or missing safety symbols can cause incorrect actions lead-
ing to personal injury or material damage! The safety symbols must not be removed! Dam-
aged safety symbols must be replaced without delay!
The following safety symbols have been attached to the PCR UV3HEPA Systems:
General warning
Caution: Radiation, ultra-
violet
3.4 Technical condition
The PCR UV3HEPA Systems correspond in their design and construction to the current
state of the art technology. Unauthorized modifications or changes, especially such that af-
fect the safety of the staff and the environment, are generally not allowed.
Observe the following notes:
The operator must only operate the device in a sound and operationally safe condi-
tion. The technical condition must always comply with the legal requirements and
regulations.
Prior to every use the device must be checked for damage and sound condition.
Any changes in the device affecting its safety must be reported by the operating
personnel to the operator without delay.
3.5 Requirements for the operating personnel
Observe the following notes:
The device must only be commissioned, operated and serviced by trained person-
nel instructed in technical safety.
The operation or servicing of the device by minors or individuals under the influ-
ence of alcohol, drugs or medication is not permitted.
It must be ensured that only authorized personnel works at the device.
The operating personnel must be familiar with the dangers arising from samples to
be used. The appropriate protective equipment must be used.
The unit includes shortwave UV, which is a powerful source of UV radiation that
will cause damage to unprotected eyes and skin. Before operating any unit, ensure
all personnel in the area are properly protected and that instructions for use of this
equipment are followed.

Safety instructions
PCR UV3 HEPA Systems Edition 09/2013 9
3.6 Safety instructions – transport and assembly
Only transport the device when it is completely empty. Clean and decontaminate
the chamber.
Allow for a sufficient cool-down of the light tubes before transport.
To prevent health damage the following notes must be observed when moving the
device in the laboratory (lifting and carrying):
– For reasons of safety 2 persons are required to transport the device and must
position themselves on both sides of the equipment.
– Because the device does not feature any handles, firmly grip the device from
the bottom and make sure prior to simultaneous lifting the door is closed.
Disconnect the power supply before fully assembling the PCR system.
Two people are recommended for assembly to avoid damage to the device.
3.7 Safety instructions – operation
The PCR UV3HEPA Systems are designed with function, reliability, and safety in mind. The
unit includes shortwave UV, which is a powerful source of UV radiation that will cause dam-
age to unprotected eyes and skin. A safety shut-off switch automatically turns the UV light off
when the door is open, protecting users from UV exposure. The Makrolon enclosure is UV
and beta-blocking and effective for protection against 32P labeled compounds.
For your own safety observe the following notes:
The device must only be operated if all protective equipment is present, properly
installed and fully operational. Special care must be taken that the panel door is
closed. Protective and safety equipment must never be removed, modified or de-
commissioned during operation.
The sound condition of the protection and safety equipment must be checked regu-
larly. Any defects must be corrected as soon as they occur.
Even though the PCR UV3HEPA Systems shut off when the panel door is opened,
UV Blocking Eyewear should be worn during operation.
The PCR UV3HEPA Systems must only be connected to a power supply with the
indicated voltage (100 V, 110 V or 230 V).
Free access to the power plug on the back of the unit must be ensured.
Covered vents may cause the device to break down or may cause damage to it.
When PCR equipment is operated inside the PCR chamber, combined Amps total
is not to exceed 8 Amps.
Avoid exposing the Makrolon panels to heaters or other heat sources, as damage
may occur. Do not use a heater exceeding 800 Watts, which can cause the internal
chamber to reach a temperature to exceed 140°C.

Safety instructions
10 Edition 09/2013 PCR UV3 HEPA Systems
3.8 Handling of auxiliary and operating materials
The operator is responsible for the selection of substances used in the process as well as for
their safe handling. This is particularly important for radioactive, infectious, poisonous, corro-
sive, combustible, explosive and otherwise dangerous substances.
Observe the following notes:
Hazardous substances have to be handled according to the biosafety level of the
laboratory. The relevant regulations and the notes in the EC safety data sheets of
the manufacturers have to be observed as well as the national and international
guidelines (WHO, “Laboratory Biosafety Manual”).
Wear protective equipment when operating the UV light.
Obey all security instructions for decontaminating the PCR UV3HEPA Systems.
3.9 Safety instructions – maintenance
Observe the following notes:
Disconnect the power supply before servicing the PCR UV3HEPA Systems. Re-
member esp. to unplug the unit before cleaning around the UV tube contacts and
internal plug outlets.
Allow for a sufficient cool-down of the light tubes before maintaining them.
The door and side panels of the PCR UV3HEPA Systems are made of Makrolon and are
subject to scuffing and scratches if improperly cleaned. The stainless steel and aluminum
surfaces are manufactured with an antimicrobial coating to reduce bacterial growth. Care in
cleaning and use of the equipment is recommended to reduce wear of the coating.
To clean the unit:
Wipe excess water from inside the unit and outside the unit with an absorbent soft
cloth or sponge.
Use only mild soap and a damp soft cloth or damp sponge to clean the exterior of
the unit. Use a mild detergent on the interior surfaces.
Never use organic based compounds, Alcohol, or Ammonia containing cleaners.
Do not use abrasive pads or cleansers.

Safety instructions
PCR UV3 HEPA Systems Edition 09/2013 11
3.10 Behavior during emergencies
In case of emergency disconnect immediately the plug of the PCR UV3HEPA Sys-
tems from the outlet!
Because a rapid response can save lives during an emergency, the following has to be en-
sured:
The operating staff must be familiar with the location of safety equipment, accident
and danger alarms, first aid and rescue equipment as well as their handling.
The operator is responsible for the respective training of the operating staff.
All equipment for first aid (first-aid kit, eyewash bottles, stretcher, etc.) as well as
equipment for firefighting (fire extinguishers) must be within reach and easy to ac-
cess. All equipment has to be in a sound condition and should be checked at regu-
lar intervals.

Technical description
12 Edition 09/2013 PCR UV3 HEPA Systems
4 Technical description
The PCR UV³ HEPA Systems create an ideal environment for preparing PCR and other
samples by reducing the chance of contamination with the filtered air treated by the
HEPA/UV system which blows into the PCR chamber.
The built-in high intensity shortwave UV (254 nm) provides a source for inactivation of DNA
between experiments. Additional contamination control is provided by the uniquely coated
stainless steel and aluminum design that maintains antimicrobial efficacy. The surface re-
sists growth of destructive bacteria, molds, and fungi. The Makrolon panel assembly blocks
UV under 400 nm.
The PCR UV3HEPA Systems feature:
Three-stage HEPA filter system with Pre filter, Carbon filter and HEPA filter
Overhead UV for decontamination of the chamber or PCR equipment
UV safety switch and keylock for preventing exposure to UV
Antimicrobial coated stainless steel and aluminum for reducing bacterial growth
Easy-access door
Overhead white light
Shelves for placement of tools
Plug outlets built into the chamber interior
Built-in UV/Air Circulator
Power plugs built into the chamber allow use of shakers, rockers, and other equipment for
PCR experiments inside the PCR chamber. The equipment can be decontaminated between
experiments.
The UV/Air Circulator module eliminates amplicons of DNA or genomic DNA from a previ-
ously dispensed experiment.

Operational ratings
PCR UV3 HEPA Systems Edition 09/2013 13
5 Operational ratings
Unit intended to be used indoors
Altitude must not exceed 2000 m
Ambient temperature not to exceed 5 °C to 40 °C (41 °F to 104 °F)
Relative humidity not to exceed 80 % for temperatures up to 31 °C (88 °F) de-
creasing linearly to 50 % relative humidity at 40 °C (104 °F)
Mains supply voltage fluctuations up to ±10 % of the nominal voltage
Pollution rating 2 or better for laboratory areas
Part Number Voltage Max Amps
849-00002-2
849-00002-3
849-00002-4
849-00002-5
230 V
230 V
115 V
110 V
8.7
8.7
9.1
9.1
849-00006-2
849-00006-3
849-00006-4
849-00006-5
230 V
230 V
115 V
110 V
8.7
8.7
9.1
9.1

Assembling the PCR UV3 HEPA Systems
14 Edition 09/2013 PCR UV3 HEPA Systems
6 Assembling the PCR UV3HEPA Systems
Carefully remove the unit from the packaging materials. Follow the assembly instructions
provided in this manual.
Tools required:
Philips head screwdriver
Flathead screwdriver
Gloves are provided to reduce transfer of fingerprints to PCR components
ATTENTION
Two people are recommended for assembly or lifting of this equipment.
Place the PCR base on a level surface large enough to accommodate the entire base. The
surface must be level to ensure proper alignment of screws with Makrolon panels.
Assembly for both the Workstation and Cabinet are the same. The assembly instruction fig-
ures provided in this manual show the Workstation.
Attaching the outer back panel
The back and inner panels and shelf bracket are assembled at the factory for shipping pur-
poses. The Makrolon frames (2) are taped to the panels.
1. Disassemble and remove the protective white film prior to completing the assembly
instructions below.
2. Attach the outer back panel to the base with two screws placed as shown below.
IMPORTANT:Do not tighten the screws at this time.
1 outer back panel
2 screws
3 base
NOTE:The notch in the top
of the outer back panel al-
lows room for the wires.
Figure 1 Attaching the outer back panel

Assembling the PCR UV3 HEPA Systems
PCR UV3 HEPA Systems Edition 09/2013 15
Attaching the inner back panel
ATTENTION
Two people are required to hold the panels in place and insert the screws.
3. Position the wires so that they run between the inner back panel and the outer back
panel up to the top for later connections to the top assembly.
4. Position the inner back panel so that the screw holes are aligned with the outer back.
Attach the inner back panel to the outer back panel with four (4) screws.
5. Secure the shelf bracket in front of the inner back panel using:
– Eight (8) screws in the Workstation
– Six (6) screws in the Cabinet
IMPORTANT: Do not fully tighten screws at this time.
1 inner back panel
2 screws
3 shelf bracket
NOTE: Position the inner
panel so that the edge of the
frame rests against the
stainless steel base.
Figure 2 Attaching the inner back panel

Assembling the PCR UV3 HEPA Systems
16 Edition 09/2013 PCR UV3 HEPA Systems
Assembling the Makrolon panels
The frames (two) are designed to hold the Makrolon panels in place against the back as-
sembly.
6. Loosen the four screws indicated in the drawing below.
7. Slide the frames for Makrolon panels under the screws on the back panel assembly so
that the frames are under the shelf brackets.
IMPORTANT: Do not tighten the screws fully.
8. Slide the Makrolon panels into position between the bracket and the frame.
9. Press the frames tightly against the Makrolon panels.
10. Tighten all screws.
1 frame for Mak-
rolon panels
2 shelf bracket
3 screws
4 Makrolon panel
Figure 3 Assembling the Makrolon panels

Assembling the PCR UV3 HEPA Systems
PCR UV3 HEPA Systems Edition 09/2013 17
Assembling the top housing and the shelves
WARNING
Before assembling top and shelves ensure that the unit is not plugged in.
11. Rest the top assembly on top of the unit.
12. Connect the bottom and top assembly wires together according to their labels, matching
wire “A” with wire “A,” wire “B” with wire “B” and so forth, until all labeled wires are con-
nected. Connect the wires by joining the male and female red connectors located on the
ends of each wire. Then, connect the two unlabeled green/yellow ground wires together
using the red connectors.
Figure 4 Connecting the bottom and top assembly wires together
13. Slide the top assembly down so that the Makrolon panels are inside the top assembly
channels. Make sure that back flange of the top assembly is positioned between the in-
ner back and the outer back panel. Secure and tighten the top assembly to the back
panel with screws, pushing from the back to ensure the screws are secure.
14. Slide the lower portion of the shelves behind the shelf bracket.
1 top assembly
2 screws
3 shelf bracket
NOTE: The flange is locat-
ed at the inside of the top
assembly.
Figure 5 Positioning the top assembly

Assembling the PCR UV3 HEPA Systems
18 Edition 09/2013 PCR UV3 HEPA Systems
Assembling the door
15. Slide the door assembly up into channel in the top assembly. Holding the door in posi-
tion, insert the thumb screws (Qty. 3) inside the top assembly.
16. Secure the door with brackets onto the left and right side panels.
17. A magnet is attached to the bottom right bracket on the door. The purpose of the mag-
net is to automatically shut off the overhead UV light when the door is open.
1 thumb screws
2 door assembly
3 side brackets
4 magnet
Figure 6 Assembling the door
18. When the PCR is assembled, plug the power cord into the back of the unit and then into
a wall outlet in the laboratory.

All parts of the PCR UV3HEPA System are assembled. The system is ready
for operation now.