Dionex AS-HV User manual

AS-HV Autosampler
Operator's Manual
Document No. 065125
Revision 01
October 2006

© 2006 by Dionex Corporation
All rights reserved worldwide.
Printed in the United States of America.
This publication is protected by federal copyright law. No part of this publication
may be copied or distributed, transmitted, transcribed, stored in a retrieval system, or
transmitted into any human or computer language, in any form or by any means,
electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties
without the express written permission of Dionex Corporation, 1228 Titan Way,
Sunnyvale, California 94088-3603 U.S.A.
DISCLAIMER OF WARRANTY AND LIMITED WARRANTY
THIS PUBLICATION IS PROVIDED “AS IS” WITHOUT WARRANTY OF
ANY KIND. DIONEX CORPORATION DOES NOT WARRANT,
GUARANTEE, OR MAKE ANY EXPRESS OR IMPLIED
REPRESENTATIONS REGARDING THE USE, OR THE RESULTS OF THE
USE, OF THIS PUBLICATION IN TERMS OF CORRECTNESS, ACCURACY,
RELIABILITY, CURRENTNESS, OR OTHERWISE. FURTHER, DIONEX
CORPORATION RESERVES THE RIGHT TO REVISE THIS PUBLICATION
AND TO MAKE CHANGES FROM TIME TO TIME IN THE CONTENT
HEREINOF WITHOUT OBLIGATION OF DIONEX CORPORATION TO
NOTIFY ANY PERSON OR ORGANIZATION OF SUCH REVISION OR
CHANGES.
TRADEMARKS
Chromeleon® is a registered trademark of Dionex Corporation. Self-Regenerating
Suppressor™ and SRS™ are trademarks of Dionex Corporation.
MASTERFLEX® C/L® is a registered trademark of Barnant Company.
Microsoft® Windows® XP and Windows® 2000 are registered trademarks of
Microsoft Corporation.
Nalgene® is a registered trademark of Nalge Nunc International.
PEEK™ is a trademark of Victrex PLC.
Plexiglas® is a registered trademark of Rohm & Haas Company.
Santoprene® is a registered trademark of Advanced Elastomer Systems, L.P.
Tefzel® is a registered trademark of E.I. duPont de Nemours & Company.
PRINTING HISTORY
Revision 01, October 2006

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Contents
1 • Introduction
1.1 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2 About This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.2.1 Safety Messages and Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.3 Safety Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
2•Description
2.1 AS-HV Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2 AS-HV Side Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
2.3 Sample Loading Modes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
2.4 Sample Loading Pumps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.4.1 Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
2.4.2 AXP Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
2.4.3 External Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
2.4.4 Internal Peristaltic Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.4.5 Relay-Controlled Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
2.5 Chromeleon Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17

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3 • Operation and Maintenance
3.1 Safety Guidelines . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
3.2 Getting Ready to Run . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
3.2.1 Filling Sample Containers and Loading the Racks . . . . . . . . . .22
3.2.2 Turning On the Power . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
3.2.3 Selecting the Rinse Pump Speed . . . . . . . . . . . . . . . . . . . . . . . .24
3.2.4 Creating a Chromeleon Program . . . . . . . . . . . . . . . . . . . . . . . .25
3.3 Routine Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
3.3.1 Daily Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
3.3.2 Weekly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.3.3 Monthly Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
3.3.4 Periodic Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
4 • Troubleshooting
4.1 Liquid Leaks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
4.1.1 Leaking Fitting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
4.1.2 Leaking Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .39
4.1.3 Leaking Waste Line (Fixed Rinse Reservoir) . . . . . . . . . . . . . .40
4.2 Sampling Arm Jams . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
4.3 Initialization Sequence Fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
4.4 Power LED Fails to Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
4.5 CPU Activity LED Fails to Light . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .43
4.6 Power Supply Fails . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .44
4.7 Cavitation in the Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .45

Contents
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5•Service
5.1 Cleaning the Syringe Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
5.2 Replacing the Sample Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 48
5.2.1 Removing the Existing Syringe . . . . . . . . . . . . . . . . . . . . . . . . 48
5.2.2 Filling the New Syringe and Removing Bubbles . . . . . . . . . . . 49
5.2.3 Installing the New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
5.2.4 Initializing the New Syringe . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
5.3 Replacing the Sampling Needle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
5.3.1 Removing the Existing Sampling Needle . . . . . . . . . . . . . . . . 52
5.3.2 Installing the New Sampling Needle . . . . . . . . . . . . . . . . . . . . 53
5.4 Replacing the Syringe Pump Distribution Valve . . . . . . . . . . . . . . . . . 55
5.4.1 Removing the Existing Valve . . . . . . . . . . . . . . . . . . . . . . . . . . 55
5.4.2 Installing the New Valve . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
5.5 Replacing the Main Power Fuses . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
A • Specifications
A.1 Electrical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
A.2 Environmental . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
A.3 Physical . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
A.4 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60

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B • Installation
B.1 Facility Requirements . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
B.2 Unpacking the AS-HV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .61
B.3 Installing the AS-HV . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
B.3.1 Installing the Spill Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .62
B.3.2 Installing the Rack Location Mat. . . . . . . . . . . . . . . . . . . . . . . .63
B.3.3 Installing the Fixed Rinse Reservoir . . . . . . . . . . . . . . . . . . . . .64
B.3.4 Connecting the Rinse Pump . . . . . . . . . . . . . . . . . . . . . . . . . . . .65
B.3.5 Installing the Standards Rack. . . . . . . . . . . . . . . . . . . . . . . . . . .66
B.3.6 Installing the Sample Rack. . . . . . . . . . . . . . . . . . . . . . . . . . . . .67
B.3.7 Installing the Trace Analysis Cover (Optional) . . . . . . . . . . . . .67
B.3.8 Installing the Sampling Needle . . . . . . . . . . . . . . . . . . . . . . . . .68
B.3.9 Connecting the Syringe Pump (Optional) . . . . . . . . . . . . . . . . .71
B.3.10 Connecting the AXP Pump (Optional). . . . . . . . . . . . . . . . . . . .73
B.3.11 Connecting the External Peristaltic Pump (Optional) . . . . . . . .75
B.3.12 Connecting the Internal Peristaltic Pump (Optional) . . . . . . . . .77
B.3.13 Connecting a Relay-Controlled Pump (Optional) . . . . . . . . . . .77
B.3.14 Connecting the AS-HV to the Chromeleon PC . . . . . . . . . . . . .78
B.3.15 Connecting the Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . .79
B.3.16 Configuring the AS-HV in Chromeleon. . . . . . . . . . . . . . . . . . .80
B.3.17 Removing Bubbles from the Syringe. . . . . . . . . . . . . . . . . . . . .82

Contents
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C • Error Messages and Codes
C.1 Audit Trail Messages . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
C.2 AS-HV ERROR Indicator Codes . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
D • Reordering Information

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1 • Introduction
1.1 Overview
The Dionex AS-HV Autosampler is a random access robotic device that can be
commanded to execute a series of liquid handling steps. The AS-HV can hold up
to 250 mL of sample and, through the use of a sample loading pump, deliver
sample to an ion chromatography (IC) system or other analyzer. Sample loading
pump options include a syringe pump, piston pump, or peristaltic pump (for
details, refer to Section 2.4).
The AS-HV is designed to accommodate a variety of accessories, including a
sample rack, a standards rack, and a rinse reservoir. The standard configuration
for the AS-HV includes the following components:
•A 24-position sample rack that accommodates 250 mL Nunclon sample flasks
•An 11-position standards rack for loading of standards and/or samples
•A PEEK™sampling needle for aspirating sample from sample containers
•A sampling needle guide that prevents the PEEK sampling needle from
bending or flexing as it pierces a septum
•A rack location mat that houses the sample rack and ensures alignment of the
sampling needle over each vial
•A built-in rinse station (consisting of a peristaltic pump and a 150 mm
reservoir that circulates rinse fluid) that can be used to clean the sampling
needle between samples
•A spill tray for secondary containment of samples
•An RS-232 cable for communication with a PC (personal computer)
The AS-HV is usually controlled remotely from a PC running Microsoft®
Windows® XP or Windows® 2000 operating system and Dionex Chromeleon®
Chromatography Management System software. The AS-HV communicates with
the PC via the RS-232 interface. Limited remote control is also available, using
relay signals.

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1.2 About This Manual
Chapter 1
Introduction An overview of the AS-HV; includes a brief description of the standard AS-HV configuration and the software
required for operation. An explanation of the conventions
used in this manual (including safety-related information).
Chapter 2
Description Descriptions of physical aspects of the AS-HV, including mechanical components and the LEDs, controls and
connectors on the side panel; an explanation of sample
loading modes; and an introduction to Chromeleon
software.
Chapter 3
Operation and
Maintenance
Procedures to complete before beginning operation,
instructions for creating Chromeleon programs for AS-HV
control, and routine preventive maintenance requirements.
Chapter 4
Troubleshooting Minor problems that may occur during operation, with step-by-step procedures for how to isolate and eliminate
the cause of each problem.
Chapter 5
Service Step-by-step instructions for routine service and parts replacement procedures for the AS-HV.
Appendix A
Specifications Specifications and installation site requirements for the AS-HV.
Appendix B
Installation Installation instructions for the AS-HV.
Appendix C
Error Messages
and Codes
Chromeleon Audit Trail error messages and AS-HV
ERROR indicator codes, along with an explanation of the
possible cause of each problem and the corrective action to
take.
Appendix D
Reordering
Information
Spare parts for the AS-HV.

1 • Introduction
Doc. 065125-01 10/06 3
1.2.1 Safety Messages and Notes
This manual contains warnings and precautionary statements that can
prevent personal injury and/or damage to the AS-HV when properly
followed. Safety messages appear in bold type and are accompanied by
icons, as shown below.
Messages d’avertissement en français
Warnhinweise in Deutsch
Indicates an imminently hazardous situation which, if not avoided, will
result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided, may
result in death or serious injury.
Indicates a potentially hazardous situation which, if not avoided, may
result in minor or moderate injury.
Indicates that the function or process of the instrument may be
impaired. Operation does not constitute a hazard.
Signale une situation de danger immédiat qui, si elle n'est pas évitée,
entraînera des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures graves à mortelles.
Signale une situation de danger potentiel qui, si elle n'est pas évitée,
pourrait entraîner des blessures mineures à modérées. Également
utilisé pour signaler une situation ou une pratique qui pourrait
gravement endommager l'instrument mais qui n'entraînera pas de
blessures.
Bedeutet unmittelbare Gefahr. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.

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Informational messages also appear throughout this manual. These are
labeled NOTE and are in bold type:
NOTE NOTES call attention to certain information. They alert
the user to an unexpected result of an action, suggest
how to optimize instrument performance, etc.
1.3 Safety Labels
The TUV GS and cTUVus Mark safety labels and the CE Mark label on the AS-
HV indicate that the AS-HV is in compliance with the following standards and
directives: EN 61010-1:2001 (safety), CAN/CSA-C22.2 No. 61010-1:2004
(safety), UL 61010-1:2004 (safety), EN 61326 1997 + A1:1998 + A2:2001
(EMC), Low-Voltage Equipment Directive 73/23/EEC, and EMC Directive
89/336/EEC.
These symbols appear on the AS-HV or on AS-HV labels:
Alternating current
Protective conductor terminal
Power supply is on
Indicates a potential hazard. Refer to the operator’s manual for
an explanation of the hazard and how to proceed.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zum Tod oder
schwerwiegenden Verletzungen führen.
Bedeutet eine mögliche Gefährdung. Mißachtung kann zu kleineren
oder mittelschweren Verletzungen führen. Wird auch verwendet, wenn
eine Situation zu schweren Schäden am Gerät führen kann, jedoch
keine Verletzungsgefahr besteht.
˜

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2 • Description
2.1 AS-HV Overview
Figure 2-1 is an overview of the main features of the AS-HV Autosampler.
Figure 2-1. AS-HV Autosampler
Power
LED
Rinse Pump
Fixed Rinse
Reservoir
Sample Rack
Rack Location Mat Base Support Tube
Rinse Pump
Speed Knob
Standards Rack
Sampling Arm
Support
Sampling Needle
Sampling Needle
Guide
Sampling Arm
Nunclon Sample
Flasks

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Power LED
The power LED lights when power to the AS-HV is turned on, and should remain
lit as long as the main power supply is operational. If the LED fails to light when
the power is turned on, refer to Section 4.4 for troubleshooting help.
NOTE The power switch is located on the AS-HV side panel
(see Section 2.2).
Sample Rack
The sample rack houses the sample containers. The AS-HV is shipped with a 24-
position sample rack (P/N 064233) designed to accommodate 250 mL Nunclon
sample flasks (flasks: P/N 064053, pkg. of 50; caps and septa: P/N 064235; pkg.
of 50).
All sample container caps contain a split septum that allows the PEEK sampling
needle to pierce the cap, while also maintaining sample integrity and minimizing
sample evaporation.
Optional: A 15-position sample rack (P/N 064234) that accommodates 250 mL
narrow-mouth Nalgene® bottles (bottles: P/N 064236, pkg. of 72; caps and septa:
P/N 064232, pkg. of 72).
Rack Location Mat
The rack location mat (P/N 064250) is mounted on the spill tray. The mat houses
the sample rack and provides calibration coordinates for properly aligning the
sampling needle over a sample container.
Spill Tray
The spill tray (P/N 064251) provides secondary containment for sample
containers in the autosampler.
Standards Rack
Standards and/or samples can be loaded onto the standards rack. The AS-HV is
shipped with an 11-position standards rack (P/N 064253).
Optional: A 6-position standards rack (P/N 064252).

2 • Description
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Sampling Needle
The sampling needle is used to aspirate sample from sample containers.
The AS-HV is shipped with a PEEK Sampling Needle Kit (P/N 064511). The kit
includes the sampling needle (a piece of specially pre-bent 0.8-mm (0.030-in) ID
PEEK tubing), fittings, and mounting hardware.
The PEEK tubing is not only the sampling needle, but the point at which the
sample is introduced to the IC. This transfer mechanism minimizes contamination
that can be caused by fittings, extra tubing, and manual sample handling.
Optional: A Sampling Needle Kit–PTFE Lined and Sleeved (P/N 064056)
containing a stainless steel needle that is lined and sleeved with PTFE
(polytetrafluoroethylene).
Sampling Needle Guide
The AS-HV is shipped with a sampling needle guide (P/N 064519). The guide
serves two purposes:
•To ensure that the PEEK sampling needle does not bend or flex as it pierces
the septum in the sample container cap.
•To ensure that the sample container is not lifted out of the sample rack during
sampling.
Rinse Station
The rinse station cleans the sampling needle between samples. During the
cleaning cycle, the needle is inserted into the rinse fluid, fluid is drawn and then
dispensed through the needle, and the dispensed fluid is directed to waste. The
rinse station consists of:
•A 150 mm reservoir of rinse fluid. Dionex recommends using ASTM Type I
(or better) filtered, deionized water as the rinse fluid.
•A variable-speed peristaltic pump that provides a supply of fresh rinse fluid to
the reservoir. The potentiometer knob fitted above the pump is used to adjust
the flow rate (see Section 3.2.3).
If an external rinse source is available, the reservoir can be connected directly to
this source and the peristaltic pump can be replumbed for operation as the sample
loading pump. For more information, refer to Section 2.4.4.

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2.2 AS-HV Side Panel
This section describes (from top to bottom) the components located on the right
side panel of the AS-HV.
ERROR Indicators
If a fault occurs, the ERROR indicators light to
display a binary error code (in blue) that identifies
the fault. For example, the error code 0110 indicates
that Error 6 (an X-axis position error) has occurred. Refer to Section C.2 for an
explanation of all of the error codes.
RESET Switch
Pressing the RESET switch returns the AS-HV to its
initial start-up sequence. The reset procedure identifies
problems with autosampler movement in the X-, Theta-, and Z-axes (see
Figure 3-1). Successful completion of the reset procedure confirms that the
motors operate and the action of the carriages is correct.
A small pointed device is required to depress the recessed switch.
DIP-A DIP Switches
The DIP switches are numbered 1 to 8 (from top to bottom).
SW5 sets the baud rate (the only communication parameter
that can be set on the AS-HV).
The table below lists the DIP switch functions and default
settings. The switch positions are indicated as 0 = Off
(Up/Open) or 1= On (Down/Closed).
DIP Switch Default Setting Description
SW1 Off Self-test mode
SW2 Off Reserved
SW3 Off Reserved
SW4 Off Reserved
SW5 On Baud rate
SW6 Off Mode selection
SW7 Off Mobile rinse station expiration timer
SW8 Off Wash select MRS/FWR (mobile rinse
station/fixed rinse reservoir)

2 • Description
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I/O Port
The I/O port provides eight contact closure inputs and four relay
outputs.
The autosampler has four internal relays (A, B, C, and D).
Normally open and normally closed contact pairs are available on
the external I/O connector.
Relay A is reserved for control of the internal peristaltic pump.
Relay B is reserved for control of an external relay-controlled
pump operating as the sample loading pump. The relays can be
controlled through commands programmed in Chromeleon.
RELAY Status Indicators
These indicators light (yellow) to indicate the
state of the internal relays (A, B, C, and D).
When a relay is energized, the corresponding
indicator is lit.
The relay outputs can be used to connect and control low power devices. These
outputs should be limited to loads of 1 amp or less. The eight digital inputs
available on the autosampler allow monitoring of electrical signals between 0 and
+24 volts.
System Status Indicators
These indicators light (yellow) to identify certain
autosampler conditions. For details, see the table below.
.
Status Indicator Description Indication
S1 Programmable behavior
LED ON when programmed
AUX Auxiliary instrument port
activity LED ON when a byte is sent or received on Auxiliary Instrument Port, then
OFF
HOST Host interface port
activity LED ON when a byte is sent or received on Host Interface Port, then OFF
CPU Central processor unit
(CPU) activity LED OFF for 10 seconds, then ON for 1 second

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AUX (Auxiliary Communications) Port
The AUX port provides a standard RS-232 interface connection
from the AS-HV to the optional syringe pump or AXP pump.
The port supports a baud rate of 9600 and operates as
connected DCE (Data Computer Equipment); the pumps
operate as DTE (Data Terminal Equipment). The RS-232 cable
required to connect the pump to the AUX port is provided in
the pump Ship Kit.
HOST (Host Communications) Port
The HOST port provides a connection from the AS-HV to a
serial port on the Chromeleon PC. The AS-HV Ship Kit
(P/N 064051) includes an RS-232 cable (P/N 060683) for this
connection.
If a serial port is not available on the PC, order the optional
RS-232-to-USB cable (P/N 064261) and use this cable to
connect the autosampler to a USB port on the PC.
Power Entry
The power entry incorporates the AS-HV main power switch and
the fuse compartment.
The AS-HV operates from input voltages over a range of 100 to
240 VAC, 50 to 60 Hz power, and requires a grounded, single-
phase power source. The typical input power is 75 W and the
maximum line draw is 1.8 amps at 110 VAC (when the power is
turned on). The appropriate line voltage and frequency are
selected automatically.
SHOCK HAZARD—To avoid electrical shock, use a grounded
receptacle. Do not operate the AS-HV or connect it to AC power mains
without an earthed ground connection.
The power supply cord is used as the main disconnect device. Make
sure the socket-outlet is located near the AS-HV and is easily
accessible.

2 • Description
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Fuse Compartment
The fuse compartment contains two slow-blow fuses rated at 5 amps
(P/N 064248). For instructions on how to replace the fuses, refer to Section 5.5.
Operation at AC input levels outside of the specified operating voltage
range may damage the AS-HV.
DANGER D'ÉLECTROCUTION—Pour éviter toute électrocution, il faut
utiliser une prise de courant avec prise de terre. Ne l'utilisez pas et ne
le branchez pas au secteur C.A. sans utiliser de branchement mis à la
terre.
Le cordon d'alimentation principal est utilisé comme dispositif
principal de débranchement. Veillez à ce que la prise de base soit
située/installée près du module et facilement accessible.
STROMSCHLAGGEFAHR—Zur Vermeidung von elektrischen
Schlägen ist eine geerdete Steckdose zu verwenden. Das Gerät darf
nicht ohne Erdung betrieben bzw. an Wechselstrom angeschlossen
werden.
Das Netzkabel ist das wichtigste Mittel zur Stromunterbrechung.
Stellen Sie sicher, daß sich die Steckdose nahe am Gerät befindet und
leicht zugänglich ist.
DANGER D'ÉLECTROCUTION—Pour éviter toute électrocution, il faut
utiliser une prise de courant avec prise de terre. Ne l'utilisez pas et ne
le branchez pas au secteur C.A. sans utiliser de branchement mis à la
terre.
Le cordon d'alimentation principal est utilisé comme dispositif
principal de débranchement. Veillez à ce que la prise de base soit
située/installée près du module et facilement accessible.
For continued protection against risk of fire or shock, replacement
fuses must be the type and rating specified in this manual.

AS-HV Operator’s Manual
12 Doc. 065125-01 10/06
2.3 Sample Loading Modes
The AS-HV supports two sample loading modes:
•In the push mode, sample enters the sample loading pump and is then pushed
into the sample loop or concentrator column.
•In the pull mode, sample is pulled into the sample loop or concentrator
column via the sample loading pump.
The sample loading mode is determined by the components that are installed and
the plumbing connections; for schematics showing typical configurations, refer to
Appendix B.
Sample Overlap
To reduce the time between injections, some Dionex devices, including the AS-
HV, support overlapping sample preparation. This means that, while data
collection and analysis for the current sample are still in progress, the AS-HV can
perform sample preparation steps for the next sample. When the autosampler
finishes the overlapped functions, the Chromeleon program waits, if necessary, to
finish the currently running sample. Then, the remaining commands in the
overlapped sample's program are executed.
Sample overlap is enabled in the Chromeleon Program Wizard (see Section 3.2.4).
Pour maintenir la protection contre les risques d'incendie ou
d'électrocution, remplacez toujours les fusibles par des fusibles du
même type et du même calibre.
Zum Schutz vor Feuer und Stromschlägen müssen beim
Sicherungswechsel immer Sicherungen des gleichen Typs und mit
gleicher Leistung verwendet werden.