Analytic Jena InnuPure C16 User manual

InnuPure C16
Extraction system
Operating manual

Manufacturer
CyBio AG
Goeschwitzer Strasse
07745 Jena
Germany
Phone:
Hotline: + 49 (0) 3641 / 351-0
Fax: + 49 (0) 3641 / 351-409
Email:
[e-mail]
Service:
Analytik Jena AG
Life Science
Konrad
-Zuse-Str. 1
07745 Jena
Germany
Phone:
Hotline: + 49 (0) 3641 / 77-94 11
Fax: + 49 (0) 3641 / 77-76 77 76
Email:
[e-mail]
General information about Analytik Jena AG
on the internet: http://www.analytik-jena.de
Copyrights and Trademarks
Microsoft and Windows are registered trademarks of Microsoft Corp.
The identification with ® or TM is omitted in this manual.
Documentation number: 30-7115-002-23
Edition – June 2016
Implementation of the Technical Documentation:
Analytik Jena AG
This publication describes the state of this product at the time of publishing. It need not necessarily
agree with future versions of the product.
Modifications reserved!
© Copyright 2016 Analytik Jena AG

Contents
InnuPure C16 Edition 06/2015 1
Contents
1Basic information ........................................................................................................ 5
1.1 User manual notes .............................................................................................. 5
User manual conventions.................................................................................... 5
1.2 Intended use........................................................................................................ 5
1.3 Warranty and liability........................................................................................... 6
2Technical data.............................................................................................................. 7
3Safety instructions ...................................................................................................... 9
3.1 General notes...................................................................................................... 9
3.2 Symbols and signal words used in the user manual........................................... 9
3.3 Safety markings on the InnuPure C16 .............................................................. 10
3.4 Technical condition............................................................................................ 11
3.5 Requirements for the operating personnel........................................................ 11
3.6 Safety instructions, transport and installation.................................................... 11
3.7 Safety instructions - operation........................................................................... 12
3.8 Handling hazardous substances....................................................................... 12
3.9 Chemical resistance of the device..................................................................... 13
3.10 Behavior during emergencies............................................................................ 13
3.11 Standards and directives................................................................................... 14
4Technical description................................................................................................ 15
4.1 Basic system design.......................................................................................... 15
4.2 Design, connections, indications....................................................................... 15
4.3 Principle of operation......................................................................................... 19
5First commissioning.................................................................................................. 22
5.1 Site requirements .............................................................................................. 22
5.1.1 Installation conditions........................................................................................ 22
5.1.2 Space requirement............................................................................................ 22
5.1.3 Energy supply.................................................................................................... 23
5.2 Connecting the InnuPure C16 and switching it on/off ....................................... 23
Connecting the InnuPure C16........................................................................... 23
Switching on the InnuPure C16......................................................................... 23
Switching off the InnuPure C16......................................................................... 24
Switching off the touch sensor .......................................................................... 24
6Operation.................................................................................................................... 25
6.1 Preparation of the buffers and solutions............................................................ 25
6.2 Preparing the sample trays and inserting them into the InnuPure C16 ............ 25
6.3 Operating InnuPure C16 with HID-Pro 320....................................................... 29
6.3.1 Overview of the InnuPure C16 program............................................................ 29

Contents
2 Edition 06/2015 InnuPure C16
6.3.2 Starting and executing an extraction protocol ................................................... 30
6.3.3 Copying, moving or deleting extraction protocols.............................................. 34
6.3.4 Checking and executing individual device functions with tools......................... 36
6.3.5 Using the sample setup tool .............................................................................. 37
Creating and saving a sample setup via the HID-Pro 320 ................................ 37
Creating a sample setup via an external PC..................................................... 39
Loading a sample setup via the HID-Pro 320.................................................... 41
6.3.6 Recording, downloading and displaying logfiles................................................ 42
Recording logfiles.............................................................................................. 42
Downloading logfiles to a USB stick.................................................................. 42
Viewing logfiles.................................................................................................. 42
6.3.7 Working with user management........................................................................ 44
User rights ......................................................................................................... 44
Creating/changing/deleting a user profile.......................................................... 45
Switching user login at start on/off..................................................................... 46
Logging in a different user................................................................................. 46
6.3.8 Enabling the interior light and the touch sensor................................................ 46
6.3.9 Configuring the time .......................................................................................... 47
7Fault removal.............................................................................................................. 48
7.1 Error messages ................................................................................................. 48
7.2 General information and tips for preventing problems ...................................... 49
8Maintenance and care ............................................................................................... 51
8.1 Cleaning the InnuPure C16............................................................................... 51
8.2 Changing fuses.................................................................................................. 52
8.3 Software update................................................................................................. 53
9Transport and storage............................................................................................... 54
9.1 Transport ........................................................................................................... 54
9.1.1 Preparing the device for transport..................................................................... 54
9.1.2 Transport notes.................................................................................................. 57
9.1.3 Moving the device in the laboratory................................................................... 57
9.2 Storage .............................................................................................................. 58
10 Disposal...................................................................................................................... 59
11 EC Declaration of Conformity................................................................................... 60

Figures
InnuPure C16 Edition 06/2015 3
Figures
Figure 1 Safety symbols at the front and rear of the InnuPure C16 .................................. 10
Figure 2 Front view of the InnuPure C16........................................................................... 16
Figure 3 Rear connections and switches at the InnuPure C16.......................................... 16
Figure 4 Device view with open front door......................................................................... 17
Figure 5 Sample tray.......................................................................................................... 17
Figure 6 Priming station and optionally available perforation tool ..................................... 18
Figure 7 HID-Pro 320......................................................................................................... 18
Figure 8 Start window......................................................................................................... 24
Figure 9 Program overview of the InnuPure C16............................................................... 29
Figure 10 Assignment of the sample positions .................................................................... 37
Figure 11 Empty sample setup in the PC program.............................................................. 40
Figure 12 Sample setup created by PC/laptop .................................................................... 41
Figure 13 Example logfile of a demo protocol run ............................................................... 43
Figure 14 Switching the interior light/enabling the touch sensor.......................................... 46
Figure 15 Window for configuring the date and time ........................................................... 47
Figure 16 Open fuse holder.................................................................................................. 52

Figures
4 Edition 06/2015 InnuPure C16

Basic information
InnuPure C16 Edition 06/2015 5
1 Basic information
1.1 User manual notes
The InnuPure C16 is intended for operation by qualified specialist personnel observing this
user manual.
The user manual informs about the design and operation of the InnuPure C16 and provides
the personnel familiar with the isolation and purification of nucleic acid with the necessary
know-how for the safe handling of the equipment and its components. The user manual fur-
ther includes notes on the maintenance and service of the equipment and information on
potential causes and remedies of any faults.
User manual conventions
Instructions for action which occur in chronological order are numbered and combined into
action units and furnished with the corresponding results.
Lists which are not in chronological order are shown as itemized lists, sub-listings as bullet
points.
Safety notes are indicated by pictographs and signal words. The type and source of the
danger, as well as the consequences, are stated together with notes on preventing the dan-
ger. The meaning of the pictographs and signal words used is explained in the chapter
"Safety notes".
The elements of the control program are indicated as follows:
Program terms are identified with SMALL CAPS.
Buttons are shown by square brackets (e.g., [OK] button)
Menu items are separated by arrows (e.g., FILE OPEN).
1.2 Intended use
The InnuPure C16 is a flexible and efficient extraction system for the fully automated isola-
tion and purification of nucleic acid in chemical and biological laboratories. In the field of
medicine and diagnostics its use is limited to research.
The InnuPure C16 including its original accessories must only be used for the applications
described in this instruction manual. The manufacturer does not accept liability for any other
use, including that of any individual modules or components.
The device must only be operated by qualified and trained personnel.
The device must only be used in accordance with this instruction manual. This ap-
plies in particular to the adherence to the connection values, conditions of use and
notes on maintenance, service, transport and disposal.
The safety notices in this instruction manual must be observed.
It is not permissible
to work with explosive substances in this device.

Basic information
6 Edition 06/2015 InnuPure C16
to operate this device in an explosive environment.
to use consumables that are not recommended for the InnuPure C16 and the cor-
responding kits.
to use overfilled consumables.
The operator is responsible for the use of the equipment as intended.
1.3 Warranty and liability
The warranty and liability periods comply with the legal requirements and the provisions in
the general terms and conditions of Analytik Jena AG.
Deviations from the intended use described in this user manual will result in limitations of
warranty and liability in the event of a claim. Damage to wearing parts or breakage of glass
are not included in the warranty.
Warranty and liability claims are excluded for personal injury and property damage due to
one or several of the following causes:
use of the InnuPure C16 other than intended
improper commissioning, operation and servicing of the device
modifications to the equipment without prior consultation with Analytik Jena AG
unauthorized intervention in the equipment
operation of the device with faulty safety equipment or improperly fitted safety and
protection equipment
inadequate monitoring of the equipment components subject to wear
use of other than original spare parts, wearing parts or consumables
improper repairs
faults due to the non-observance of this user manual

Technical data
InnuPure C16 Edition 06/2015 7
2 Technical data
System parameters
Extraction principle based on surface-functionalized magnetic or paramagnetic parti-
cles
Extraction duration from 28 min (dependent on the source material)
Drive 5 low-noise long-life servomotors
Device operation stand-alone via HID-Pro 320 with 5.7” touchscreen (color)
Maintenance maintenance-free through the use of non-wearing stainless steel
pistons
Cleaning system components easily accessible through the front door
Temperature control heating up to 80°C
Application parameters
Consumables completely contained in the required kit
sealed, pre-filled reagent strips or plates
Lysis step automated in the device (dependent on the source material)
Extraction routines pre-installed protocols (optimized for the most varied source mate-
rials)
Piercing function removing the sealing film from the reagent strips and plates not
necessary
Cross contamination completely excluded through sealed reagent strips and plates
Tips max. 32 per preparation each with a capacity of 1000 µl
Samples
Sample capacity up to 16 preparations in parallel
processing of different source materials possible with identical pi-
petting protocols
Sample preparation pre-insertion of the pipette tips, samples and elution tubes into the
sample tray
insertion of pre-filled reagent strips/plates into the sample tray
Sample volume up to 2 ml or up to 50 mg
Elution separate storage vessels with cover
Elution volume adjustable from 20 - 500 µl or 50 – 500 µl depending on the proto-
col
Accessories and kits
Sample tray carrier for the pre-insertion of all consumables and samples
Priming station rack for easy preparation of the sample tray
Kits DNA extraction and RNA extraction from different source materials
Piercing tool(optional) tool for manually opening the strips and plates at the tip ejection
positions 1 and 3
Adapter for reagent
strips (optional) adapter for accepting a maximum of 4 reagent strips
Barcode scanner reading the sample names from barcode labels
Other technical data
Weight approx. 35 kg
Dimensions
(W x H x D) 380 mm x 435 mm x 530 mm

Technical data
8 Edition 06/2015 InnuPure C16
Electrical variables
Power supply 110 – 230 V AC, 50 – 60 Hz
Protection 2 x T 4A H 250V
Max. power consump-
tion 160 VA
PC interface USB
Interference suppres-
sion
(electromagnetic
compatibility)
interference suppression in compliance with the requirements of
BMPT Vfg. 243/1991 and EN 55022 Class A
Environmental conditions
Temperature during
operation 5 – 35 °C
Humidity during op-
eration max. 80% at +30 °C
Temperature during
storage -10 – 55 °C
Humidity during stor-
age max. 80% (use desiccant)