4 CALIBRATION
4.1 Preparation for Calibration
a) Calibration point
The instrument has 5 calibration points: 0 NTU, 20 NTU, 100 NTU, 400 NTU, and 800 NTU.
Among them, 0 NTU point uses AMCO 0.0 NTU calibration solution or laboratory distilled water,
and the remaining 4 calibration points use AMCO polymer solutions. Note that the cap of the 0.0
NTU solution cuvette can be unscrewed. After the solution is invalidated, users can replace the
0.0 NTU calibration solution or use fresh laboratory distilled water. The AMCO 0.0 NTU
calibration solution refill can be purchased from the supplier; For the remaining 4 calibration
solutions, their cuvette caps cannot be opened. Simply dispose the solutions after they are
expired and buy new ones from your supplier to replace. See the detail in Section 8 Replacement
Parts.
b) Replace 0.0 NTU turbidity standard solution
•Open the 0.0 NTU cuvette cap, pour out the original solution, add 1/2 distilled water, screw
on the cap and shake the cuvette to rinse it off and pour out the water. Repeat it 3 times.
Shake off the distilled water in the cuvette. Pour in new AMCO 0.0 NTU calibration solution
or fresh laboratory distilled water. Then close the cuvette cap.
•AMCO 0.0 NTU calibration solution has 6 to 12 months of shelf-life, distilled water is only
valid for a couple of days.
AMCO 0.0 NTU solution is provided in the default kit. For purchase details, please refer to Section 8
Replacement Parts.
c) Clean cuvette surface
Apply a small drop of silicone oil on the surface of the cuvette and wipe it off with a lint-free cloth to
evenly distribute the silicone oil on the surface in order to cover smudges and scratches,which helps
light scattering. But please pay attention to the following points:
①The silicone oil applied should not be too much. After wiping with lint-free cloth, please wipe with
filter paper or high-quality tissue paper to clean off. Excessive residual silicone oil on the cuvette
surface will affect the measurement accuracy.
②It's not necessary to use silicone oil for each calibration and measurement. Apply silicone oil
every several days or once a week. In between, just clean the surface with filter paper or high-
quality tissue paper.
③Clean the calibration cuvette and sample cuvette together and keep the steps and actions
consistent to achieve same degree of cleanliness.
④Stability of calibration solutions
The U.S EPA approved AMCO polymer standard calibration solution is very uniform and stable.
It does not precipitate, drift or condense. Generally, it can be used directly without shaking or
flipping the cuvette (to make the solution even). For AMCO polymer calibration solutions that
have not been used for a long time, slowly flip the cuvette twice and let it stand for 2 minutes.