axolotl BIOSYSTEMS axo User manual

AXOLOTL BIOSYSTEMS
Industry and Trade Limited Company
User Manual

Axolotl Biosystems is the producer of dierent products aimed for
researchers working in regenative medicine and tissue engineering.
AxoCell Stimulator is a biomaterial property testing system specifically designed for
researchers who work with 3D Bioprinted tissues. AxoCell Stimulator is a bioreactor that can
deform flexible membranes and 3D matrices in a sterile fluid environment.
Axolotl Biosystems focuses on providing the set of tools that makes regenative medicine and
tissue engineering research easier, faster and reliable. Our technologies and products
advance researchers in these fields and oer a set of plug & play devices.
2

CONTENTS
GENERAL
INFORMATION 1
GETTING
STARTED 2
3
BIOMATERIAL
TESTING
6 - 7
8 - 13
14 - 19
Product Map
Setting Up
Software Installation
Dismounting
Technical Specifications
General Safety Information
Electrical and Environmental Information
Maintenance
Warranty
Biomaterial Testing
Functions: Sine&Ramp
Test Blocks and Protocols
Loading Specimen to Device
Software Overview
Origin, Jog and Maximum Amplitudes
Setting Up & Running a Test
3

4
GENERAL
INFORMATION 1

GENERAL
INFORMATION 1
5
1. GENERAL INFORMATION
1.1 TECHNICAL SPECIFICATIONS
AxoCell Stimulator is a precision instrument developed for deforming bioprinted tissues,
flexible membranes and 3D matrices in a strerile flued environment. This device is capable
of uniaxial deformations on flexible cell-seeded substrates or scaolds. It has an onboard
actuator and control board to enable it to run independent of a computer in an
environment-controlled incubator.
Stimulation Uniaxial Tension
Cell Culture Area 6 strips up to 90mm
Maximum Strain >%100
Maximum Velocity 10mm/s
Maximum Cycle Frequency 5Hz
Loading Capacity 250N
Media Volume 100-385mL
Weight 3100gr
Cell-Contacting Components Autoclavable Materials
Product Construction Stainless Steel
Standalone Working Available
Connection USB
Workspace 80 mm
Dimensions 530x210x130 mm

GENERAL
INFORMATION 1
6
Electrical Input 12VDC
Current Rating 5 Amps
Electrical Supply Connection 100-240 VAC - Grounded
Pollution Degree Degree 2
Environment Conditions Maximum Operating Temperature 40ºC
0% - 100% Relative Humidity
Installation Category Category II
1.2 GENERAL SAFETY INFORMATION
1.3 ELECTRICAL AND ENVIRONMENTAL INFORMATION
To meet the necessary standarts of safety, all personnel using the device must read the user
manual.
If you come up with any problems that is not addressed in the manual, reach out to Axolotl
Biosystems.
Please use the device according to the manual. Do not try to modify the parts.
Attention! The device must be used according to its purpose and should be
be used in an other way stated in this manual. Any modification to the parts
and wrong usage of the device will revoke warranty. Reach Axolotl
Biosystems about any problem you might have with your device.
Attention! AxoCell Stimulator has moving parts while working. Do not place
your hands inside the testing chamber during the testing procedure.
Attention! Make sure the electrical connections are done as stated in the
manual. All connections must be grounded. Electrical connections should
remain dry for safety.

GENERAL
INFORMATION 1
7
1.4 MAINTENANCE
The equipment does not require maintenance other than cleaning. All parts can
be cleaned with ethanol on the exterior. Testing Chamber, Pull Rod, and Clamps
can be sterilized with UV.

GETTING
STARTED 2
8

GETTING
STARTED 2
2.1 PRODUCT MAP
End Clamp
Specimen Clamp
Power Input
USB Cable
9
Power Button
Main Chamber
Rod Lock
Test Chamber
Tension Rod
Chamber Lock
End Lock

GETTING
STARTED 2
10
2.2 SETTING UP
To set up your product, use the power cable provided and plug the device using
Power Input socket.
2.3 SOFTWARE INSTALLATION
AxoCell Stimulator Software enables you to control your device from your computer via
USB connection. To use your instrument, you have to install the software provided to you
in an email link or USB. Note that AxoCell Stimulator only works on its own software and
other softwares may cause your product to malfunction and cause damage out of
warranty.
Click on the setup software.
1
2
Install software. (For more information, Check out 2.3 Software Installation)
1
Start the device using power button.
3
Choose your setup language.
2
Choose the folder you wan to install
the software.
3