BACTcontrol microLAN User manual

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 1 of 59
User Manual
Version: V0.4.4
Date: 01.11.2020

Page 2 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 3 of 59
1. TABLE OF CONTENTS
1. TABLE OF CONTENTS ........................................................................3
LIST OF TABLES...........................................................................................6
1.1 VERSION OVERVIEW ...........................................................6
2. GENERAL.............................................................................................7
2.1 SAFETY..................................................................................7
2.2 COPYRIGHT ..........................................................................8
2.3 WARRANTY...........................................................................8
2.4 MANUAL INFORMATION.......................................................9
2.4.1 NOTES, CAUTIONS AND WARNINGS..................................9
2.5 ISO 15839 ..............................................................................9
2.6 RELATED DOCUMENTS.......................................................9
3. INTRODUCTION OF THE BACTCONTROL.......................................11
4. TECHNICAL DETAILS........................................................................12
4.1 CONDITIONS.......................................................................12
4.2 MATERIAL AND WEIGHT....................................................12
4.3 FLOOR SPACE BACTCONTROL........................................13
4.4 SAMPLE DETAIL..................................................................13
4.5 ELECTRICAL CONDITIONS................................................14
4.6 ENVIRONMENTAL CONDITIONS .......................................14
4.7 SPECIFICATIONS OPTIONS...............................................14
4.8 GENERAL COMPUTER SPECIFICATIONS ........................14
4.9 SYSTEM SPECIFICATIONS................................................15
4.10 SCREEN...................................................15
4.11 CONNECTIONS TOUCH PANEL .............16
4.12 COMMUNICATION SPECIFICATIONS ....16
5. MEASUREMENT SPECIFICATIONS..................................................17
5.1 THE ANALYZER...................................................................18
5.2 REACTION CHAMBER ........................................................19
5.3 PHOTODIODE SENSOR UNIT............................................20
5.4 TEMPERATURE SENSOR ..................................................20
5.5 PRESSURE SENSOR..........................................................20

Page 4 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
5.6 BUBBLE SENSOR ...............................................................21
5.7 PUMP UNITS........................................................................21
5.7.1 SAMPLE PUMP....................................................................21
5.7.2 REAGENTS PUMPS............................................................21
5.8 REAGENTS..........................................................................22
5.8.1 EXPLANATION SUBSTRATE..............................................23
5.8.2 STORAGE AND USE OF THE REAGENTS ........................24
6. BACTCONTROL INSTALLATION......................................................25
6.1 CONNECTIONS ON THE BACTCONTROL.........................25
6.2 CONNECTION OF THE WATER SAMPLE INLET, OUTLET &
WASTE.......................................................................................26
6.3 CONNECTING ALARMS AND ANALOG OUTPUT..............27
6.4 POWER SUPPLY.................................................................28
6.5 CHANGING/REPLACING FUSES........................................28
7. INTRODUCTION TO THE BACTCONTROL SOFTWARE..................29
7.1 LOG IN .................................................................................29
7.2 STATUS ...............................................................................29
7.3 ALARMS...............................................................................30
7.4 DETAILS...............................................................................31
7.5 SETTINGS............................................................................32
7.5.1 SEQUENCE..........................................................................32
7.5.2 CYCLE..................................................................................32
7.5.3 PUMP...................................................................................33
7.5.4 CLEANING ...........................................................................33
7.5.5 MISC.....................................................................................34
7.5.6 MAIL34
7.5.7 CHANGE A VALUE IN A SUBMENU: ..................................35
7.6 START A MEASUREMENT..................................................36
7.7 STOP A MEASUREMENT....................................................37
7.8 SERVICE..............................................................................38
7.9 RESULTS.............................................................................39
7.10 DATA EXPORT.........................................40
8. MAINTENANCE SCHEDULE..............................................................42
8.1 DAILY MAINTENANCE ........................................................42
8.1.1 VISUAL CHECK ...................................................................42
8.1.2 VOLUME SOLUTIONS.........................................................43
8.1.3 SUPPLY AND WASTE CONTAINERS.................................44
8.1.4 DATA CHECK.......................................................................44
8.2 MONTHLY MAINTENANCE.................................................44

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 5 of 59
8.2.1 CLEANING REACTION CHAMBER.....................................45
8.3 QUARTERLY MAINTENANCE.............................................47
8.3.1 REPLACE TUBING .............................................................47
8.3.2 PERISTALTIC PUMP TUBE CHANGING ............................47
8.4 PREVENTIVE MAINTENANCE (YEARLY) ..........................48
9. REAGENTS, CLEANING SOLUTION AND BUFFERS.......................49
10. CONSUMABLES.................................................................................50
11. SPARE PARTS ...................................................................................51
12. SOFTWARE........................................................................................52
APPENDIXA:GENERALWORKINGOFTHEBACTCONTROL.............................54
APPENDIX B: CHECKLIST .........................................................................58

Page 6 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
LIST OF TABLES
Table 1 Version overview...................................................................................................... 6
Table 2 Measurement specifications ....................................................................................17
Table 3 Recommended storage and use of reagents...........................................................24
Table 4 reagents, solutions and buffers................................................................................49
Table 5 Consumables ..........................................................................................................50
Table 6 Spare parts..............................................................................................................51
Table 7 Software..................................................................................................................52
1.1 VERSION OVERVIEW
Table 1 Version overview
Version
Release date
Changes
V0.4.1
30-05-2016
Basic version
V0.4.1
21-09-2016
Preliminary version
V0.4.2
25-01-2017
Preliminary version
V0.4.3
16-09-2019
Draft version
V0.4.4
28-10-2020
Updated

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 7 of 59
2. GENERAL
2.1 SAFETY
Figure 1 Wash hands
Water supply can be contaminated with bacteria!
Always wash your hands before and after working with the BACTcontrol.
Figure 2 Caution, risk of electric shock
Figure 3 Hot surface
Always switch off the instrument during maintenance and cleaning.

Page 8 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
2.2 COPYRIGHT
This manual and all containing information and figures are copyrighted. All rights
(publishing, reproduction, printing, translating, storage) are reserved by microLAN b.v.
Each reproduction or utilization outside the permitted limits of the copyright law are not
allowed without previous written consent of microLAN b.v.
The reproduction of products names, registered trade names, designation of goods
etc. in this manual does not imply that these names can be used by everyone, often
these are registered trademarks, even if they are not marked as such.
2.3 WARRANTY
microLAN b.v. warrants each Model BACTcontrol and its optional equipment against
defects in materials and workmanship under normal use and service for a period of
one (1) year. Equipment installed by microLAN is warranted from the installation date;
all other equipment is warranted from the ship date. If purchaser schedules or delays
installation more than 90 days after delivery, then the warranty period starts on the
91st day from date of shipment. This warranty extends only to the original purchaser.
microLAN will, at its option, repair or replace equipment that proves to be defective
during the warranty period, provided the equipment is returned to microLAN at the
expense of the purchaser. Parts, labour, and return shipment to the customer shall be
at the expense of microLAN. Travel costs shall be at the cost of the purchaser.
Software and firmware designed by microLAN for use with an external PC will execute
its programming instructions when properly installed on that PC. microLAN does not
warrant that the operation of the PC, software, or firmware will be uninterrupted or
error-free.
Consumables and parts are warranted for 30 days (parts only) and are not available
for coverage under extended warranties or service contracts.
This warranty shall not apply to defects originating from:
Improper maintenance or operation by purchaser.
Purchaser-supplied accessories or consumables.
Modification or misuse by purchaser.
Operation outside of the environmental and electrical product specifications.
Improper or inadequate site preparation.
Purchaser-induced contamination or leaks.

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 9 of 59
2.4 MANUAL INFORMATION
2.4.1 NOTES, CAUTIONS AND WARNINGS
This manual contains Notes, Cautions and Warnings,
for situations that may:
Endanger operating personnel,
Cause damage to equipment
Need specific attention.
The following formats and symbols are used:
NOTE
Notes provide additional information, such as expanded explanations, hints or
reminders.
CAUTION
Cautions alert you to conditions that may cause damage to the equipment or
interfere with the normal processing and damage the product.
WARNING
Warnings point out procedures you must follow precisely to avoid personal
injury or serious damage to the equipment.
2.5 ISO 15839
ISO 15839 describes the procedure and performance of on-line sensors/analyzing
equipment for water. This standard is applicable to most sensors/analyzing
equipment.
This International Standard :
Defines an on-line sensor/analyzing equipment for water quality measurements;
Defines terminology describing the performance characteristics of on-line
sensors/analyzing equipment;
Specifies the test procedures (for laboratory and field) to be used to evaluate the
performance characteristics of on-line sensors/analyzing equipment.
2.6 RELATED DOCUMENTS
BACTcontrol Software Manual
BACTcontrol Advanced user Manual
BACTcontrol Quick user Manual

Page 10 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
Manual Optional Cooling-Unit

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 11 of 59
3. INTRODUCTION OF THE BACTCONTROL
The determination of the presence of microorganisms is a key parameter in order to
determine whether a water body is safe, yet the standard determination methods that
are used are based on the cultivation of these microorganisms. These methods are
characterized of being time consuming (24-48) and are not suitable for a fast water
quality assessment (REF. 3). Due to these time consuming procedures, there is an
considerable chance that populations get exposed to microbiological hazards, before
the results of the tests are known (REF. 1). Microbiological contamination is a
constant concern in a wide range of fields. For example water treatment, food
industries and pharmaceutical companies are fields where fast detection of dangerous
microorganisms that might cause diseases is extremely important. Diseases that are
present in water bodies can be prevented by monitoring (REF. 1). Enzymatic activity
determination is one detection process that is suitable for rapid measurements of
dangerous microorganisms (REF. 3). Unfortunately there is still a significant lack of
automated on-line systems for the detection of microbial parameters, which speed up
the detection process of dangerous microorganisms (REF. 3).
The BACTcontrol is an on-line automated instrument for the detection of
microbiological activity in water. It measures the specific enzymatic activities of β-
galactosidase (coliforms), β-glucoronidase (E.coli), ß-glucosidase (Enterococci) and
alkaline phosphatase (Total Activity, biomass), as an indicator for the presence of
bacterial contamination. The enzyme activity is detected by adding reagents
(consumables) which contain a fluorescent indicator. The reagents are substrate
specific for the enzyme to be detected, meaning that fluorescence will increase when
the corresponding enzyme is present in the sample.
Figure 4 BACTcontrol
The BACTcontrol is an “Early warning system”, complementing the officially accepted
methods for the detection of microbiological activity. The measurements are realized
in a short period of time (1-2 hours), in contrast to classical microbiological methods,
which are labour intensive and in which cultivation of the organisms is required, taking
several hours before getting reliable results (24-48).
In Appendix B a more detailed explanation about enzymes and the working of the
BACTcontrol is given.

Page 12 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
4. TECHNICAL DETAILS
4.1 CONDITIONS
The BACTcontrol is specially designed for use on a table or on a trestle.
Place the BACTcontrol in a properly ventilated area
Make sure the BACTcontrol setup is at least 20 cm from a wall
The humidity conditions in the area should be between 20 and 80 %
Keep the BACTcontrol out of direct solar irradiation
Protect the BACTcontrol from rain
The area temperature should be between 15–35°C / 59 –86°F. In case of higher
temperatures, an optional Cooling-Unit is recommended (Part nr: 01BACT001A516)
Figure 5 Optional enclosure Cooling-Unit (Part nr: 01BACT001A516)
4.2 MATERIAL AND WEIGHT
Cabinet: Stainless steel 316
Protection classification: IP 54 (IP 65 optional)

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 13 of 59
Weight : 25kg (excl. optional cooling-unit 30kg in total)
4.3 FLOOR SPACE BACTCONTROL
Depth: 321 mm (347 mm incl. hinges & door handle)
Width: 450 mm ( 482 mm incl. connectors)
Height: 460 mm (470 mm incl. rubber feet)
Figure 6 Dimensions BACTcontrol
4.4 SAMPLE DETAIL
Sample inlet
Sample connection: 4 mm ID
Tube size: 2,5 mm ID x 4,0 mm OD
Sample pressure: max 0.05 bar
Sample flow: 3l/h
Sample temperature: 10- 35°C / 50 –95°F
Second inlet/ Extra rinsing
Sample connection: 4 mm ID
Tube size: 2,5 mm ID x 4,0 mm OD
Sample pressure: max 0.05 bar
Sample flow: 3l/h
Sample temperature: 10- 35°C / 50 –95°F
Sample outlet and waste
Sample connection: 4 mm ID

Page 14 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
Tube size: 2,5 mm ID x 4,0 mm OD
Sample flow: 3l/h
4.5 ELECTRICAL CONDITIONS
Operation voltage: 230V - 50Hz or 110V –60Hz
Nominal power consumption: <50W (without optional cooling unit)
Peak power consumption: <700W (included optional cooling-unit)
It is recommended to protect the electrical supply with an earth leakage circuit breaker
4.6 ENVIRONMENTAL CONDITIONS
Place the BACTcontrol in a properly ventilated area
Temperature between 15–30°C / 59 –86°F, (at higher temperatures, an optional
Cooling-unit is recommended)
No direct solar irradiation
Protect from rain
Humidity conditions between 20 and 80 %
4.7 SPECIFICATIONS OPTIONS
Modem slot for UMTS, ISDN or analog (modem optional)
Second sample inlet / extra rinsing
Cooling-Unit (if higher than 30°C / 86°F)
4.8 GENERAL COMPUTER SPECIFICATIONS
Integrated PC with Windows professional SP1 operating system
Graphical user interface with touchscreen
Touchscreen power DC12V/5A

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 15 of 59
4.9 SYSTEM SPECIFICATIONS
Processor: Onboard Intel® Cedarview dual core D2550 (1.86GHz,1MB L2 cache,
10W) mobile Processor
Chipset: Intel® NM10 Chipset
Ram: Onboard 2GB DDR3 1066MHz Memory
Graphics: Intel CedarView Integrated Graphics Engine with GMA3650
IP65: YES
Power: DC12V/5A Power Adapter
2 x USB 2.0 type A
2 x LAN 10/100/1000MB/s; RJ-45
1 x DB-9 RS-232/422/485 COM1; default RS-232
1 x DB-9 RS-232; COM2
Protocols: Modbus, others on request
1x 4 - 20mA output
Operating system: English
4.10 SCREEN
Integrated PC with Windows
Graphical user interface with touchscreen
LCD Size: 8,4 inch
Display type: XGA LCD
Resolution: 800 x 600
Display color: 262K
Viewing Angle: 65/65/65/45
Brightness: 300cd / C m2
Contrast: 500:1
Touchscreen: Five-wire resistive touch screen

Page 16 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
4.11 CONNECTIONS TOUCH PANEL
Figure 7 Connections touch panel
1On/Off button
2 Com port (COM1)
3USB ports (4)
(one port is used for software log, other three ports are available for external use)
4Network connections (2)
5 Com port (COM2)
6 Power input DC12V/5A
4.12 COMMUNICATION SPECIFICATIONS
Full network capability via direct LAN
Data output:
Digital: Modbus
Analog: 1x 4 - 20mA output

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 17 of 59
5. MEASUREMENT SPECIFICATIONS
Table 2 Measurement specifications
Parameter
E. coli
Coliform
Total activity
(TA)
Enterococci
Temperature
measurement
44,0 °C
111,2 °F
36,0 °C
96,8 °F
45,0 °C
113,0 °F
37,0 °C
98,6 °F
Reactor
chamber size
2ml
10ml
2ml
10ml
2ml
10ml
2ml
10ml
Buffer dosing
[µL]
60
250
60
250
-
-
60
250
Reagent
dosing [µL]
60
250
120
500
60
250
60
250
pH
6,9
6,9
10,0
8,8
Stabilization
time [min]
20
20
20
20
Incubation
time [min]
20
20
20
20
Enzyme
ß-
glucuronidase
ß-
galactosidase
alkaline
phosphatase
ß-glucosidase
Substrate
fluorescent
compound
4-
Methylumbellif
eryl β-D-
glucuronide
4-
Methylumbellif
eryl ß-D-
galactopyrano
side
4-
Methylumbellif
eryl
phosphate
4-
Methylumbellif
eryl ß-D-
glucopyranosi
de

Page 18 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
5.1 THE ANALYZER
Figure 8 Overview inside BACTcontrol (frontside)
1Valves
2Reaction chamber
3Vent hole
4Reagent and buffer pumps
5Cleaning solution, buffer and reagents
6Sample pump
7Bubble sensor
8Pressure senor
1
2
3
4
5
7
6
8
9

microLAN b.v. BACTcontrol –User Manual –V0.4.4 Page 19 of 59
9Ventilator
5.2 REACTION CHAMBER
In the reaction chamber the measurements take place. When the concentration of
bacteria in the sample is low, the bacteria has to be concentrated in order to be able
to detect the fluorescence and have reliable results. This is done with a porous
ceramic filter (Figure 9). The sample is pumped into the reaction chamber and flows
through the ceramic filter (size 0,45 µm). The pores of the filter are small enough to
retain the bacteria and let the water flow through. When the concentration of bacteria
is high enough and there is no need to concentrate it, the dummy filter can be used
instead of the ceramic filter. The dummy filter is made from metal, so liquid cannot
flow through it. In this case the sample will be pumped to the reaction chamber and a
valve is kept open in order to let the first millilitres of sample flow through the outlet,
increasing the chance that a representative sample will be measured. When the
chamber is filled the measurement takes place.
Figure 9 Reaction chamber with ceramic filter with C-ring
After the sample has been heated up to the right temperature, the photodiode emits
light with a wavelength of 366 nm through the photodiode window into the reaction
chamber (Figure 10). As the bacteria to be determined is present in the sample, the
hydrolysed MU substrate will fluorescence (APPENDIX A: GENERAL WORKING OF
THE BACTCONTROL).
The fluorescence is detected by the photodiode and converted into enzymatic activity
and expressed in picomole per minute

Page 20 of 59 BACTcontrol –User Manual –V0.4.4 microLAN b.v.
Figure 10 Overview reaction chamber and photodiode window and stirrer
5.3 PHOTODIODE SENSOR UNIT
A photodiode is a light detector, which converts light into voltage or current, depending
on how the device is programmed. A photodiode consist of optical filters and it is built-
in lenses. The photodiode is responsible for the detection of fluorescence which is an
indication of enzymatic activity of specific bacteria (E.coli, coliform, total activity) in the
sample. The photodiode is connected to the reaction chamber, where the sample is
collected.
5.4 TEMPERATURE SENSOR
The temperature sensor ensures that the measurements are carried out at the correct
temperature. Each bacteria has their own temperature optimum, therefore it is
important to monitor it. Also in the case of the calibration it is important to have a
temperature sensor, since the calibration solution is highly sensitive to temperature
changes.
5.5 PRESSURE SENSOR
The pressure sensor is placed between the sampling pump and the reaction chamber
and monitors the sample pressure (see figure 8), which should not exceed 3 bar. After
a period of time and depending on the type of sample the ceramic filter might get
blocked by the many particles present in water, decreasing the sample flow and
increasing the sample pressure. When the pressure exceeds the maximum, the
system will send a warning and stop the measurements.