Metrohm 6.5330.210 User manual

IC equipment
IC equipment: Inline Ultrafiltration – MiPT (6.5330.210)
Manual
8.110.8039EN / 2019-10-25

Metrohm AG
CH-9100 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
IC equipment
IC equipment: Inline Ultrafiltration –
MiPT (6.5330.210)
Manual
8.110.8039EN / 2019-10-25

Technical Communication
Metrohm AG
CH-9100 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
This documentation has been prepared with great care. However, errors
can never be entirely ruled out. Please send comments regarding possible
errors to the address above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ III
Table of contents
1 Introduction 1
1.1 Description of the IC equipment: Inline Ultrafiltration –
MiPT ....................................................................................... 1
1.2 About the documentation ................................................... 2
1.2.1 Symbols and conventions ........................................................ 3
2 Overview 4
2.1 Parts of the IC equipment: Inline Ultrafiltration –
MiPT ....................................................................................... 4
2.2 Components of the ultrafiltration cell ................................ 5
2.3 Connectors of the ultrafiltration cell ................................... 6
2.4 Components of the Liquid Handling Station ...................... 7
3 Mode of operation of the intelligent Partial Loop Injection
Technique 8
4 Installation 10
4.1 Installing the ultrafiltration cell ......................................... 10
4.1.1 Preparing the ultrafiltration cell .............................................. 10
4.1.2 Connecting the ultrafiltration cell ........................................... 14
4.1.3 Inserting the ultrafiltration cell ............................................... 17
4.1.4 Deaerating the ultrafiltration cell ............................................ 17
4.2 Equipping the ultrapure water bottle ............................... 19
4.3 Installing the Dosino .......................................................... 20
4.3.1 Installing the Dosino .............................................................. 20
4.3.2 Connecting the dosing unit with the ultrapure water bottle ... 22
4.3.3 Connecting the dosing unit to the injection valve ................... 23
4.3.4 Connecting the dosing unit to the waste container ................ 24
4.4 Exchanging the sample loop ............................................. 26
4.5 Connecting the Liquid Handling Station .......................... 26
5 Operation and maintenance 29
5.1 Operation ............................................................................ 29
5.1.1 Service life of the filtration membrane .................................... 29
5.1.2 Selecting the filtration membrane .......................................... 30
5.2 Maintenance ....................................................................... 31
5.2.1 Replacing the filtration membrane ......................................... 31
5.2.2 Maintenance of the dosing unit (6.3032.120) ........................ 32

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
6 Technical specifications 33
6.1 Ultrafiltration cell (6.2729.110) ......................................... 33
6.2 Filtration membrane (6.2714.020) .................................... 33
6.3 Liquid Handling Station (6.2841.120) ............................... 33
6.4 Dosing unit 2 mL (6.3032.120) ......................................... 33
7 Accessories 34
Index 35

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of figures
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ V
Table of figures
Figure 1 IC equipment: Inline Ultrafiltration – MiPT – Overview ....................... 2
Figure 2 IC equipment: Inline Ultrafiltration – MiPT – Parts .............................. 4
Figure 3 Couplings, adapters and screws of the IC equipment: Inline Ultrafiltra-
tion – MiPT ....................................................................................... 5
Figure 4 Ultrafiltration cell – Parts .................................................................... 5
Figure 5 Ultrafiltration cell – Connectors ......................................................... 6
Figure 6 Liquid Handling Station – Parts .......................................................... 7
Figure 7 Connecting the ultrafiltration cell – Overview ................................... 10
Figure 8 Connecting the ultrafiltration cell ..................................................... 14
Figure 9 Equipping the ultrapure water bottle ............................................... 19
Figure 10 Connecting the Dosino – Overview .................................................. 20
Figure 11 Mounting the Dosino ...................................................................... 21
Figure 12 Mounting the FEP tubing ................................................................. 23
Figure 13 Installing the transfer capillary ......................................................... 24
Figure 14 Connecting to the waste container .................................................. 25
Figure 15 Connecting the Liquid Handling Station – Overview ......................... 26
Figure 16 Connecting the Liquid Handling Station ........................................... 26

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Description of the IC equipment: Inline Ultrafiltra-
tion – MiPT
The IC equipment: Inline Ultrafiltration – MiPT (6.5330.210) contains all
the required accessory parts for the Inline Ultrafiltration and for the
Metrohm intelligent Partial Loop Injection Technique (MiPT).
Combining the Inline Ultrafiltration and MiPT allows for the samples to be
filtered and subsequently be filled into the sample loop with an accurately
measured volume.
The main component of the Inline Ultrafiltration is the high-performance
ultrafiltration cell. This cell is suitable for the filtration of samples that pose
particular requirements with regard to filtration effectiveness and sample
throughput.
MiPT allows you to fill the 250 µL sample loop with a precisely measured
volume. In this process, an 800 Dosino with a 2 mL dosing unit performs
the precise dosing increments. MiPT enables calibration with only one
standard solution, as the injection volume can be selected freely. The same
applies to sample injection. This means, for example, that a small injection
volume can be selected for a highly concentrated sample.
The sample needle is rinsed with ultrapure water in the rinsing unit of the
Liquid Handling Station (6.2841.120) after each sample aspiration. The
Liquid Handling Station (6.2841.120) can be mounted on any Sample Pro-
cessor equipped with a Swing Head.

1.2 About the documentation ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
Figure 1 IC equipment: Inline Ultrafiltration – MiPT – Overview
1.2 About the documentation
This manual describes the installation of the IC equipment: Inline Ultrafil-
tration – MiPT.
CAUTION
Please read through this documentation carefully before putting the
equipment into operation. The documentation contains information
and warnings which the user must follow in order to ensure safe opera-
tion of the equipment.
Additional documentation
Topic Document
Mounting the Liquid Handling Station on
the Sample Processor
8.108.8011 Manual for Liquid Handling
Station
Installing the Dosino 8.800.8002 Manual for 800 Dosino
Care and maintenance of the dosing unit 8.807.8002 Manual for 807 Dosing Unit

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 3
1.2.1 Symbols and conventions
The following symbols and formatting may appear in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec-
ond to the instrument part in the figure.
Instruction step
Carry out these steps in the sequence shown.
Method Dialog text, parameter in the software
File ▶ New Menu or menu item
[Next] Button or key
WARNING
This symbol draws attention to a possible life-threat-
ening hazard or risk of injury.
WARNING
This symbol draws attention to a possible hazard due
to electrical current.
WARNING
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
WARNING
This symbol draws attention to a possible biological
hazard.
CAUTION
This symbol draws attention to possible damage to
instruments or instrument parts.
NOTE
This symbol highlights additional information and
tips.

2.1 Parts of the IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
2 Overview
2.1 Parts of the IC equipment: Inline Ultrafiltration –
MiPT
Figure 2 IC equipment: Inline Ultrafiltration – MiPT – Parts

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 5
Figure 3 Couplings, adapters and screws of the IC equipment: Inline
Ultrafiltration – MiPT
2.2 Components of the ultrafiltration cell
Figure 4 Ultrafiltration cell – Parts
1Stopper 2Upper chamber

2.3 Connectors of the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
3Sealing ring 4Lower chamber
5Washers 6Screws
For joining the upper and the lower part of
the cell.
2.3 Connectors of the ultrafiltration cell
Figure 5 Ultrafiltration cell – Connectors
1Upper chamber 2Lower chamber
3Filtrate outlet
Labeled OUT.
4Sample inlet
Labeled IN.
5Sample outlet
Labeled OUT.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 7
2.4 Components of the Liquid Handling Station
Figure 6 Liquid Handling Station – Parts
1Cover
For the mixing vessel.
2Rinsing unit
3Main body
With magnetic stirrer dummy.
4Rinsing unit inlet
Sealed with threaded stopper.
5Clamping fastener 6Base plate
7Platform 8Cable clip
9Waste connector 10 Mixing vessel outlet
Sealed with threaded stopper.
11 Mixing vessel

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
3 Mode of operation of the intelligent Partial
Loop Injection Technique
1. Emptying the dosing unit
At the beginning of the determination, the 2-mL dosing unit of the
Dosino is emptied.
2. Aspirating sample
The peristaltic pump between the sample changer and the ultrafiltra-
tion cell aspirates the sample.
3. Ultrafiltration
The peristaltic pump delivers a continuous flow of the sample at a
high rate through the lower chamber of the ultrafiltration cell. The
sample flows along the filtration membrane and then to the waste
container.
At the same time, the Dosino generates a vacuum in the upper cham-
ber of the ultrafiltration cell, thus aspirating the sample solution
through the filtration membrane. The filtrate enters the transfer capil-
lary.
Less than 20% of the original sample solution volume is analyzed as
filtrate. The remainder flows directly into the waste container.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 3 Mode of operation of the intelligent Partial Loop Injection Technique
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 9
4. Filling the sample loop
a. The injection valve is switched to the Fill position.
b. The 800 Dosino doses the injection volume from the transfer
capillary to the 250 µL sample loop.
The 800 Dosino doses the required ultrapure water from the
ultrapure water bottle to the transfer capillary and the sample
loop.
c. Excess sample and ultrapure water are discharged via port 4 of
the dosing unit.
5. Injecting
The injection valve is switched to the Inject position. The sample is
injected into the separation column with the eluent.
6. Rinsing
a. The needle on the Sample Processor moves to the rinse posi-
tion of the Liquid Handling Station.
b. The peristaltic pump between the ultrapure water bottle and
the Liquid Handling Station delivers ultrapure water to the Liq-
uid Handling Station.
c. The needle is rinsed in the Liquid Handling Station.

4.1 Installing the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
4 Installation
4.1 Installing the ultrafiltration cell
Figure 7 Connecting the ultrafiltration cell – Overview
4.1.1 Preparing the ultrafiltration cell
Inserting the filtration membrane
Required accessories ￭Ultrafiltration cell (6.2729.110)
￭Filtration membrane (6.2714.020)
￭5 mm hex key for IC Sample Processors (6.2621.070)

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 4 Installation
IC equipment: Inline Ultrafiltration – MiPT ￭￭￭￭￭￭￭￭ 11
￭Plastic tweezers (6.2831.010)
1Removing the stoppers
￭Remove the 3 green stoppers.
￭Turn the ultrafiltration cell around and place it on a table. The
screws face upwards.
2Removing the screws
￭Loosen the screws with the hex key.
￭Remove the screws with the washers and put them aside.
3Disassembling the ultrafiltration cell

4.1 Installing the ultrafiltration cell ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
12 ￭￭￭￭￭￭￭￭ IC equipment: Inline Ultrafiltration – MiPT
￭Remove the upper chamber of the ultrafiltration cell.
￭Remove the sealing ring.
4Cleaning the ultrafiltration cell
CAUTION
Damage to the ultrafiltration cell
Organic solvents (e.g. acetone) corrode and damage the ultrafiltra-
tion cell material (PMMA).
Use only ultrapure water or a water-ethanol mixture (70:30) for
cleaning the ultrafiltration cell.
￭Thoroughly rinse off the sealing ring, the upper chamber and the
lower chamber of the ultrafiltration cell with ultrapure water.
￭Dry all parts with a lint-free cloth.
5Wetting the filtration membrane
NOTICE
In the package containing the filtration membranes, you will find
sheets of different thicknesses and colors:
￭The firm white cardboard is a cover protecting the filtration
membranes. Do not insert the cover into the ultrafiltration cell.
￭The thin light-blue sheets are separation sheets placed between
2 filtration membranes. Do not insert the separation sheets into
the ultrafiltration cell.
￭The thin white sheets are the filtration membranes. Only insert
the filtration membranes into the ultrafiltration cell.