VWR ShiroGel Horizontal Manual, Ver.1, 08.04.2010
2. Place the comb(s) in the grooves. Each tray has more than one comb grove so that
multiple combs can be used. Using multiple combs increases sample number available
per gel but decreases run length and care must be taken to ensure that samples from the
first wells do not migrate into the lanes of the second comb wells.
3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur
can be smoothed to the edge of the gel and dispersed using a pipette tip.
4. Allow the agarose to set, on a flat surface; the agarose will become opaque once
solidified.
5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the
main tank.
Using Traditional tape method:-
1. Autoclave or plastic backed general tape should be used. A length 5cm longer than the
width of each end of the tray should be cut. One length should be placed over one end of
the tray and stuck m1cm in from the tray edge. This should then be folded and the edges
sealed securely. Repeat for the other end and place onto a level surface for gel pouring.
2. Place the comb(s) in the grooves. Each tray has more than one comb grove so that
multiple combs can be used. Using multiple combs increases sample number available
per gel but decreases run length and care must be taken to ensure that samples from the
first wells do not migrate into the lanes of the second comb wells.
3. Pour in the agarose carefully so as not to generate bubbles. Any bubbles that do occur
can be smoothed to the edge of the gel and dispersed using a pipette tip.
4. Allow the agarose to set, on a flat surface; the agarose will become opaque once
solidified.
5. Carefully remove the gel casting gates and comb and transfer the gel including tray to the
main tank.
Running the Gel:-
1. Mix the sample to be loaded with sample buffer – see page 9 solutions for common sample
buffers. Usually 3ul of sample buffer is adequate but less may be used with sample volumes of
less than 10ul.
2. Fill the unit with buffer until the gel is just flooded with buffer. This will give the fastest
resolution times. For enhanced quality of resolution of sample, fill the unit to 5mm above the gel.
Note: DNA, RNA have a net negative charge and will migrate towards the anode.
3. Load the samples into the wells using pipettes. Multi-channel pipettes can be used for
loading samples with MC compatible combs, see listing in accessories for identification of these.
