Beckman Coulter PA 800 plus User manual

A51964AC
February 2011
User’s Guide
PA 800 plus
Pharmaceutical Analysis
System
System Maintenance
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

User’s Guide
PA 800 plus Pharmaceutical Analysis System
System Maintenance
PN A51964AC February 2011)
Copyright © 2009-2011 Beckman Coulter, Inc.
Beckman Coulter, Inc. grants a limited non-exclusive
license to the owner or operator of a PA 800 plus
instrument to make a copy, solely for laboratory use,
of any portion or all of the online help and electronic
documents shipped with the PA 800 plus instrument.
Trademarks:
Following is a list of Beckman Coulter trademarks:
• Beckman Coulter®
• 32 Karat™
All other trademarks are the property of their respective
owners.
Find us on the World Wide Web at:
www.beckmancoulter.com and www.CELeader.com
Beckman Coulter Ireland, Inc.
Mervue Business Park, Mervue Galway, Ireland 353 91 774068
Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda
Estr dos Romeiros, 220 - Galpao G3 - Km 38.5
06501-001 - Sao Paulo - SP - Brasil
CNPJ: 42.160.812/0001-44
製造販売元 : ベ ッ ク マ ン･コールタ ー株式会社
東京都江東区有明三丁目 5 番 7 号
TOC 有明ウエス ト タ ワー
贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]

A51964AC iii
Revision History
Second Revision, A51964AC, February 2011
32 Karat Software version 9.1 patch
PA 800 plus Software version 1.1 patch
PA 800 plus Firmware version 9.2
Numerous syntax and grammatical edits
First Revision, A51964AB, December 2009
Revised corporate address.
Initial Issue, A51964AA, April 2009
32 Karat Software version 9.1
PA 800 plus Software version 1.1
PA 800 plus Firmware version 9.0

A51964AC
iv
Revision History

A51964AC v
Safety Notices
Symbols and Labels
Introduction
The following is a description of symbols and labels used on the Beckman Coulter PA 800 plus
Pharmaceutical Analysis System or shown in this manual.
WARNING
If the equipment is used in a manner not specified by Beckman Coulter, Inc., the
protection provided by the instrument may be impaired.
General Biohazard Symbol
This caution symbol indicates a possible biohazard risk from patient specimen contamination.
Caution, Biohazard Label
This caution symbol indicates a caution to operate only with all covers in position to decrease risk
of personal injury or biohazard.

A51964AC
vi
Safety Notices
Symbols and Labels
Caution, Moving Parts Label
This caution symbol warns the user of moving parts that can pinch or crush.
High Voltage Electric Shock Risk Symbol
This symbol indicates that there is high voltage and there is a risk of electric shock when the user
works in this area.
Class 1 Laser Caution Label
A label reading “THIS PRODUCT CONFORMS TO APPLICABLE REQUIREMENTS OF 21 CFR 1040 AT THE
DATE OF MANUFACTURE” is found near the Name Rating tag. The laser light beam is not visible.
Sharp Object Label
A label reading “CAUTION SHARP OBJECTS” is found on the PA 800 plus.
CAUTION
PARTS MOVE
AUTOMATICALLY
A015081L.EPS
144557-AB
A016352L.EPS
A016350L.EPS
CLASS 1 LASER PRODUCT
THIS PRODUCT CONFORMS TO
APPLICABLE REQUIREMENTS OF
21 CFR 1040 AT THE DATE OF
MANUFACTURE.
MANUFACTURED:
726024-C
CAUTION
SHARP OBJECTS
A16558-AA
A016351L.EPS

A51964AC vii
Safety Notices
Symbols and Labels
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates:
1. The device was put on the European Market after August 13, 2005.
2. The device is not to be disposed of via the municipal waste collection system of any member
state of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label, please contact your dealer or local Beckman Coulter office for details on the take back
program that facilitates the proper collection, treatment, recovery, recycling, and safe disposal of
this device.
Disposal of Devices Containing Mercury Components
This product contains a mercury-added part. Recycle or dispose of according to local, state, or
federal laws. It is very important that you understand and comply with the safe and proper disposal
of devices containing mercury components (switch, lamp, battery, relay, or electrode). The mercury
component indicator label can vary depending on the type of device.

A51964AC
viii
Safety Notices
Symbols and Labels
Restriction of Hazardous Substances (RoHS) Labels
These labels and materials declaration table (the Table of Hazardous Substance's Name and
Concentration) are to meet People's Republic of China Electronic Industry Standard SJ/T11364-2006
"Marking for Control of Pollution Caused by Electronic Information Products" requirements.
RoHS Caution Label
This logo indicates that this electronic information product contains certain toxic or hazardous
elements, and can be used safely during its environmental protection use period. The number in the
middle of the logo indicates the environmental protection use period for the product. The outer
circle indicates that the product can be recycled. The logo also signifies that the product should be
recycled immediately after its environmental protection use period has expired. The date on the
label indicates the date of manufacture.
RoHS Environmental Label
This logo indicates that the product does not contain any toxic or hazardous substances or
elements. The "e" stands for electrical, electronic, and environmental electronic information
products. This logo indicates that this electronic information product does not contain any toxic or
hazardous substances or elements, and is green and is environmental. The outer circle indicates
that the product can be recycled. The logo also signifies that the product can be recycled after being
discarded, and should not be casually discarded.

A51964AC ix
Safety Notices
Symbols and Labels
Alerts for Warning, Caution, Important, and Note
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. The warning can be used to indicate the
possibility of erroneous data that could result in an incorrect diagnosis (does not
apply to all products).
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. The caution can be used to indicate the possibility of erroneous data
that could result in an incorrect diagnosis (does not apply to all products).
IMPORTANT IMPORTANT is used for comments that add value to the step or procedure being performed.
Following the advice in the IMPORTANT notice adds benefit to the performance of a piece of equipment
or to a process.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.

A51964AC
x
Safety Notices
Symbols and Labels

xi
Contents
Revision History, iii
Safety Notices,v
Symbols and Labels,v
CHAPTER 1: Installation Procedures,1-1
Introduction,1-1
Install UV or PDA Detectors,1-2
Calibrate the PDA Detector,1-5
Install UV Detector Wavelength Filters,1-6
Integrated 488 nm Solid-State Laser,1-12
Installation Requirements,1-12
Install the Solid-State Laser Module,1-12
Install the LIF Detector Filters,1-13
Install the Filter Housing into the Detector,1-14
Install the LIF Detector into the PA 800 plus,1-15
Connect the Probe to the Clamp Bar,1-15
LIF Detector Calibration,1-17
Troubleshooting Procedures,1-20
Capillary Cartridge,1-21
Rebuild a Capillary Cartridge,1-21
Install a Capillary Cartridge into the PA 800 plus,1-39
Universal Vials and Caps,1-42
Fill the Universal Vials,1-42
Fill the Micro Vials,1-43
CHAPTER 2: Maintenance Procedures,2-1
Maintenance Procedures,2-1
Opening Levers, Electrodes, and the Interface Block,2-2
Clean Electrodes, Opening Levers, and the Interface Block,2-5
Coolant,2-6
Fiber Optics (UV and PDA Detectors),2-8

xii
Contents
Lamp,2-10
Instrument Care,2-17
LIF Care and Maintenance,2-17
Inspect the LIF,2-17
How to Clean the LIF,2-17
How to Store the LIF,2-17
Replace the Quad-Rings,2-18
Replace the Fuses,2-19
CHAPTER 3: Interfacing PA 800 plus with Mass Spectrometry,3-1
CE-MS/MS Mode Cartridge Procedures,3-1
How to Install the Capillary in the Standard Cartridge,3-2
How to Install the Capillary in the EDA-Only cartridge,3-3
Ground Connection,3-5
APPENDIX A: LIF Test Mix Calibration and Performance Tests,A-1
Relative Fluorescent Units (RFU),A-1
Calibration Correction Factor (CCF),A-1
Applying the CCF,A-2
Automatic Calibration,A-2
Calibration Method,A-3
Non-fluorescent Solutions,A-4
LIF Calibration for Different Dimension Capillaries,A-4
LIF Calibration for Different Fluorescent Solutions,A-5
Standard System Performance Test,A-5
Troubleshooting,A-6
APPENDIX B: Evaluating PA 800 plus UV Filters,B-1
Overview,B-1

A51964AC 1-1
CHAPTER 1
Installation Procedures
Introduction
The installation procedures covered are listed as follows. Start with the type of detector, then
continue to remaining items.
• Install UV or PDA Detectors
• Calibrate the PDA Detector
• Install UV Detector Wavelength Filters
• Install the Solid-State Laser Module
• LIF Detector Calibration
• Capillary Cartridge
WARNING
Disconnect the power before any instrument disassembly. Failure to do so can
cause electrical shock or other injury.
WARNING
Maintenance or repair procedures not specifically described in this manual
present a risk of electrical shock or injury. Refer additional servicing to Beckman
Coulter.
WARNING
Do not attempt to defeat any of the instrument interlocks or safety mechanisms.

A51964AC
1-2
Installation Procedures
Install UV or PDA Detectors
Install UV or PDA Detectors
NOTE The following procedure covers installation of the UV and PDA detectors. For the capillary cartridge
installation procedure, see Capillary Cartridge.
For information on how to install the LIF Detector, see Install the LIF Detector into the PA 800 plus
procedure.
Before you start, you need:
• UV or PDA Detector
• Fiber optic cable (UV cable is 2 ended and the PDA cable is 3 ended)
• UV source optic module installed
1 Verify that power is off and no capillary cartridge is installed.
2 Lift the cartridge cover door and find the detector mounting location as shown in Figure 1.1 and
Figure 1.2.
3 Remove the detector from the package and remove the protective connector caps.
4 Position the detector on the mounting location. Carefully slide the detector back to make
contact with the back-plane receptacle.
5 Tighten the thumb screw until tight.
6 Unpack the fiber optic cable and remove the protective covers from each end (two covers for
the UV cable and three covers for the PDA cable). Make sure to keep the protective covers for
storage when not in use. Connect the cable to the clamp arm first, then to the detector.

A51964AC 1-3
Installation Procedures
Install UV or PDA Detectors 1
Figure 1.1 Installing the UV Detector
1. Outer Door Cover
2. UV Detector
3. Two-ended Fiber Optic Cable
4. Clamp Bar
5. Capillary Cartridge
6. Interface Block

A51964AC
1-4
Installation Procedures
Install UV or PDA Detectors
Figure 1.2 PDA Detector Installation
1. Outer Door Cover
2. PDA Detector
3. Three-ended Fiber Optic Cable
4. Clamp Bar
5. Capillary Cartridge
6. Interface Block

A51964AC 1-5
Installation Procedures
Calibrate the PDA Detector 1
Calibrate the PDA Detector
The PDA detector must be calibrated following installation, after you change the lamp or other
optical components, and when required as part of maintenance.
Calibrate the PDA detector as follows:
1 From the main menu, open an instrument configured with PDA detection.
2 From the Control menu, select Direct Control. The Direct Control screen displays.
Figure 1.3 PDA Detector - Direct Control
3 Select the rainbow above the lamp. The PDA Detector Parameters screen displays.

A51964AC
1-6
Installation Procedures
Install UV Detector Wavelength Filters
Figure 1.4 PDA Detector Parameters
4 Select Calibrateto start the calibration sequence. The calibration requires approximately two
minutes. Do not try to access the instrument during the calibration process.
Install UV Detector Wavelength Filters
When you install, the PA 800 plus includes 200 nm, 214 nm, 254 nm, and 280 nm filters. The following
section is for reference.
Before you start, you need:
• Filters
• Latex gloves
1 Using Direct Control, move the trays to the load position.
2 Lift the outer door and then the cartridge cover door. The cartridge cover door is to the left of
the UV Detector as shown in Figure 1.5.

A51964AC 1-7
Installation Procedures
Install UV Detector Wavelength Filters 1
Figure 1.5 Installing UV Detector Wavelength Filters
3 Loosen the two thumb-screws and lift the clamp bar.
4 Remove the capillary cartridge from the interface block.
5 Turn the system power off.
1. Outer Door Cover
2. Three-ended Fiber Optic Cable
3. UV Detector
4. Clamp Bar
5. Capillary Cartridge
6. Interface Block

A51964AC
1-8
Installation Procedures
Install UV Detector Wavelength Filters
6 Loosen the two thumb-screws and remove the optics source assembly. Put the assembly on a
clean work surface. The optics source assembly is located right behind the interface block and
has a horizontal bar handle as shown in Figure 1.6.
7 Remove the filter wheel access cover and rotate the filter wheel until the correct filter is visible.
See Figure 1.6.
Figure 1.6 How to Remove the Filter Wheel Access Cover
8 Put on latex gloves and use small forceps to remove the desired filters from the filter wheel.
Touching the optical surfaces of the filters without gloves leaves skin oil that can cause
improper filter performance.
9 Inspect each wavelength filter for a burned spot. Replace any filters that appear damaged.
UV filters are affected by heat and time. It is good practice to periodically check filter
performance with a spectrometer or to replace the filters.
10 Insert the new filter with the reflective side facing inward. See Figure 1.7.
1. Open cover at the indicated point
2. Access Cover
3. Thumb screws (2), one on each side