Beckman Coulter EPICS XL User manual

COULTER®EPICS®XL™ Flow Cytometer
COULTER EPICS XL-MCL™ Flow Cytometer
SYSTEM IITM Software Addendum
PN 623532AA (September 2010)
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
TM
CYTOMETER
LASER
SAMPLE FLOW
LOW
READY
ON
MED HIGH
FS
SS
AUX
FL4
FL3
FL1
FL2
®

WARNINGS AND PRECAUTIONS
READ ALL PRODUCT MANUALS AND CONSULT WITH BECKMAN COULTER-TRAINED PERSONNEL BEFORE ATTEMPTING
TO OPERATE INSTRUMENT. DO NOT ATTEMPT TO PERFORM ANY PROCEDURE BEFORE CAREFULLY READING ALL
INSTRUCTIONS. ALWAYS FOLLOW PRODUCT LABELING AND MANUFACTURER’S RECOMMENDATIONS. IF IN DOUBT AS
TO HOW TO PROCEED IN ANY SITUATION, CONTACT YOUR BECKMAN COULTER REPRESENTATIVE.
BECKMAN COULTER, INC. URGES ITS CUSTOMERS TO COMPLY WITH ALL NATIONAL HEALTH AND SAFETY
STANDARDS SUCH AS THE USE OF BARRIER PROTECTION. THIS MAY INCLUDE, BUT IT IS NOT LIMITED TO,
PROTECTIVE EYEWEAR, GLOVES, AND SUITABLE LABORATORY ATTIRE WHEN OPERATING OR MAINTAINING THIS OR
ANY OTHER AUTOMATED LABORATORY ANALYZER.
HAZARDS AND OPERATIONAL PRECAUTIONS AND LIMITATIONS
WARNINGS, CAUTIONS, and IMPORTANTS alert you as follows:
WARNING - Can cause injury.
CAUTION - Can cause damage to the instrument.
IMPORTANT - Can cause misleading results.
WARNING Risk of operator injury if:
r All doors, covers and panels are not closed and secured in place prior to and during instrument operation.
r The integrity of safety interlocks and sensors is compromised.
r Instrument alarms and error messages are not acknowledged and acted upon.
r You contact moving parts.
r You mishandle broken parts.
r Doors, covers and panels are not opened, closed, removed and/or replaced with care.
r Improper tools are used for troubleshooting.
To avoid injury:
r Keep doors, covers and panels closed and secured in place while the instrument is in use.
r Take full advantage of the safety features of the instrument. Do not defeat safety interlocks and sensors.
r Acknowledge and act upon instrument alarms and error messages.
r Keep away from moving parts.
r Report any broken parts to your Beckman Coulter Representative.
r Open/remove and close/replace doors, covers and panels with care.
r Use the proper tools when troubleshooting.
CAUTION System integrity might be compromised and operational failures might occur if:
r This equipment is used in a manner other than specified. Operate the instrument as instructed in the Product Manuals.
r You introduce software that is not authorized by Beckman Coulter into your computer. Only operate your system’s
computer with software authorized by Beckman Coulter.
r You install software that is not an original copyrighted version. Only use software that is an original copyrighted
version to prevent virus contamination.
IMPORTANT If you purchased this product from anyone other than Beckman Coulter or an authorized Beckman Coulter
distributor, and, if it is not presently under a Beckman Coulter service maintenance agreement, Beckman Coulter cannot
guarantee that the product is fitted with the most current mandatory engineering revisions or that you will receive the most
current information bulletins concerning the product. If you purchased this product from a third party and would like
further information concerning this topic, call your Beckman Coulter Representative.

iii
PN 623532AA
REVISION STATUS
Initial Issue, 12/03
SYSTEM II Software, Version 3.0.
This addendum presents updated information and illustrations relative to the Universal
Power Supply (UPS).
Issue AA, 09/10
Software Version 3.0.
Updates were made to the company corporate address.
Note: Changes that are part of the most recent revision are indicated in text by a bar in the
margin of the amended page.
This document applies to the latest software listed and higher versions. When a subsequent software version
changes the information in this document, a new issue will be released to the Beckman Coulter website. For
labeling updates, go to www.beckmancoulter.com and download the most recent manual or system help for
your instrument.

PN 623532AA
iv
REVISION STATUS

PN 623532AA v
CONTENTS
WARNINGS AND PRECAUTIONS,ii
REVISION STATUS, iii
UPDATES FOR YOUR DOCUMENTATION,1
DOCUMENT OVERVIEW,1
What Documents Are Affected,1
How to Locate Related Information,1
DESCRIPTION OF CHANGES,1
XL and XL-MCL Flow Cytometry System,2
Location of Information,2
Universal Power Supply,3
Location of Information,3
Power and Signal Cable Connections,4
Location of Information,4
Waste and Pneumatic Tubing Connections,5
Location of Information,5
Component Dimensions,5
Location of Information,5
Cleaning the Air Filters,6
Location of Information,6
Prepare to Clean the Air Filters,6
Rinse and Return the Air Filters,6
Cleaning the Vacuum Trap,7
Location of Information,7
Prepare to Clean VAC TRAP,7
Rinse and Return VAC TRAP to its Bracket,7
Emptying the Waste Container,8
Location of Information,8
Adjusting the System Pressure,8
Location of Information,8
Resetting the Circuit Breakers,9
Location of Information,9
Warning Labels on UPS,12
Location of Information,12
TRADEMARKS
DOCUMENTATION PAGE

PN 623532AA
vi
CONTENTS
ILLUSTRATIONS
ILLUSTRATIONS
1 XL and XL-MCL Flow Cytometer,2
2 Universal Power Supply,3
3 Power and Signal Cable Connections, with Universal Power Supply,4
4 Waste and Pneumatic Tubing Connections, with Universal Power Supply,5
5 Location of Vacuum Trap,7
6 Location of Pressure Adjust Knob,8
7 International Warning Symbol Locations,12

PN 623532AA 1
UPDATES FOR YOUR DOCUMENTATION
DOCUMENT OVERVIEW
What Documents Are Affected
This addendum describes the updates to the documentation for the COULTER®EPICS®
XL™ and XL-MCL™ Flow Cytometers with SYSTEM II™ software.
Documents affected:
r Getting Started manual, PN 4237238.
r Reference manual, PN 4237298.
r Special Procedures and Troubleshooting manual, PN 4237296.
Keep this Addendum with your current documentation.
How to Locate Related Information
The location of the changed information is identified by chapter name, heading name, and/or
procedure name and step number as appropriate. The name of the affected manual is also
noted.
DESCRIPTION OF CHANGES
Illustrations and procedures have been updated to reflect the Universal Power Supply.

PN 623532AA
2
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
XL and XL-MCL Flow Cytometry System
Location of Information
Reference Manual:
t In the Use and Function Chapter under the heading System Components.
Getting Started Manual:
t In the Controls and Indicators Chapter under the heading XL and XL-MCL
Flow Cytometry System.
Figure 1 XL and XL-MCL Flow Cytometer
3532002A
TM
CYTOMETER
LASER
SAMPLE FLOW
LOW
READY
ON
MED HIGH
FS
SS
AUX
FL4
FL3
FL1
FL2
®
CYTOMETER
COMPUTER
WORKSTATION
MCL
OPTION
POWER
SUPPLY
MODULE
SYSTEM POPWER
MCL POPWER

PN 623532AA 3
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Universal Power Supply
Location of Information
Getting Started Manual:
t In the Controls and Indicators Chapter under the heading Power Supply.
Figure 2 Universal Power Supply
3532003A
AIR
FILTER
SYS
VAC SYS
PRESS
30
PSI
PRESS
ADJ
Gauge shows the amount
of SYStem VACuum.
Gauge shows the amount
of SYStem PRESSure.
VAC
TRAP
AIR
FILTER
VAC
FILTER
30
PSI
Refer to Special
Procedures and
Troubleshooting
manual if water is
present.
PRESSure ADJust knob
lets you change the
system pressure.
WATER
TRAP
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ

PN 623532AA
4
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Power and Signal Cable Connections
Location of Information
Reference Manual:
t In the Installation Chapter under the heading System Connections, Power and
Signal Cables.
Figure 3 Power and Signal Cable Connections, with Universal Power Supply
TX
RX
CYT12
3532006A
To optional
printer
To
keyboard
AC power
line cords
Mouse
Workstation
MCL option
To Power supply
Cytometer

PN 623532AA 5
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Waste and Pneumatic Tubing Connections
Location of Information
Reference Manual:
t In the Installation Chapter under the heading System Connections, Waste and
Pneumatic Tubing.
Figure 4 Waste and Pneumatic Tubing Connections, with Universal Power Supply
Component Dimensions
Location of Information
Reference Manual:
t In the Specifications/Characteristics Chapter under the heading Instrument
Specifications, Dimensions.
Flow cell
waste
Waste
To Waste
Pressure
Vacuum
To Power
supply
Cytometer
Component Height Width Depth Weight
Universal Power Supply 58.4 cm
(23 in.) 45.7 cm (18 in.) 71.1 cm (28 in.) 59.0 kg (130 lb)

PN 623532AA
6
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Cleaning the Air Filters
Location of Information
Special Procedures and Troubleshooting Manual:
t In the Cleaning Procedures Chapter under the heading Cleaning the Air Filters.
Prepare to Clean the Air Filters
Step 1: Turn off the system at the computer and unplug both Power Supply power cords from
the wall outlet.
Rinse and Return the Air Filters
Step 5: Plug both Power Supply power cords into the wall outlet then turn on the system at
the computer.
The instrument has four air filters. They
are located on the:
r Cytometer Back Panel
r Power Supply, Left Side Panel

PN 623532AA 7
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Cleaning the Vacuum Trap
Location of Information
Special Procedures and Troubleshooting Manual:
t In the Cleaning Procedures Chapter under the heading Cleaning the Vacuum Trap.
If you have the Universal Power Supply, refer to the following illustration when you do the
Clean the Vacuum Trap procedure.
Figure 5 Location of Vacuum Trap B:
Prepare to Clean VAC TRAP
Step 4: Unplug both Power Supply power cords from the wall outlet.
Rinse and Return VAC TRAP to its Bracket
Step 6: Plug both Power Supply power cords into the wall outlet.
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ
b

PN 623532AA
8
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Emptying the Waste Container
Location of Information
Special Procedures and Troubleshooting Manual:
t
In the Replace/Adjust Procedures Chapter under the heading Emptying the Waste Container.
Adjusting the System Pressure
Location of Information
Special Procedures and Troubleshooting Manual:
t
In the Replace/Adjust Procedures Chapter under the heading Adjusting the System Pressure.
Figure 6 Location of Pressure Adjust Knob B:
SYSTEM POPWER
MCL POPWER
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ
b

PN 623532AA 9
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Resetting the Circuit Breakers
Location of Information
Special Procedures and Troubleshooting Manual:
t In the Replace/Adjust Procedures Chapter under the heading Resetting the Circuit
Breakers.
If you have the Universal Power Supply, use the following procedure to reset the circuit
breakers.
Check the indicators bthrough the front
door of the Power Supply. If any of the
indicators are off (dark), reset the circuit
breakers.
1Open the Power Supply front door.
SYSTEM POPWER
MCL POPWER
b
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ

PN 623532AA
10
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
2Put the main switch in the 0 position to
turn the system off.
3Reset the circuit breakers by pressing
all 26 buttons.
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ
VAC
FILTER

PN 623532AA 11
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
4Put the main switch in the 1 position to
turn the system back on.
5 Power the computer and cytometer ON.
Note: If the problem persists, contact
your Beckman Coulter Representative.
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ

PN 623532AA
12
UPDATES FOR YOUR DOCUMENTATION
DESCRIPTION OF CHANGES
Warning Labels on UPS
Location of Information
Reference Manual:
t In the Precautions and Hazards Chapter under the heading Warning Labels.
The label located near the circuit breakers on the Universal Power Supply instructs the
user to refer to product documentation before resetting the circuit breakers.
The label located next to the power switch on the Universal Power Supply instructs
the user to refer to product documentation before powering up the instrument.
Figure 7 International Warning Symbol Locations
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ
SYSTEM POWER
MCL POWER
WATER
TRAP
VAC
TRAP
AIR
FILTER
VAC
FILTER
SYS
VAC
SYS
PRESS
30
PSI
PRESS
ADJ

PN 623532AA
TRADEMARKS
The BECKMAN COULTER logo, COULTER, EPICS, SYSTEM II, XL, and XL-MCL are
trademarks of Beckman Coulter, Inc.
All other trademarks, service marks, products, or services are trademarks or registered
trademarks of their respective holders.

Printed on Recycled Paper
COULTER®EPICS XL and XL-MCL Flow Cytometer Documentation
s Reference
PN 4237298
Use and Function • Installation • Operation Principles •
Specifications/Characteristics • Precautions and Hazards • Log Sheets • Predefined
Panels, Protocols, and QC Templates • Glossary • Index
s Special Procedures and
Troubleshooting
PN 4237296
Cleaning Procedures • Replace/Adjust Procedures • Troubleshooting • Bar-Code
Options • QC Maintenance Screen • Index
s Instructions for Use
PN 4237297
Startup • Quality Control • Creating a Worklist • Running Samples • Data Analysis •
Printing and Exporting Patient Reports • Reviewing QC Data • Cleaning and
Shutdown • Index
s Data Management
PN 4237237
Data Management • System Configuration • Working with QC Features • Creating
Reports • Exporting Data • File Formats • Index
s Getting Started
PN 4237238
Controls and Indicators • Learning the Basics • Software Overview • Creating
Protocols • Using Regions • Creating Panels • The Operating System • Index
s Operating Summary
PN 4237299
Quick reference versions of daily procedures.
s Master Index
PN 4237295
Combined index for the Operator’s Guide, Special Procedures and Troubleshooting,
Reference, Getting Started, and Data Management manuals.
Come visit us at www.beckmancoulter.com
Copyright © Beckman Coulter, Inc. 2003-2010
All Rights Reserved