Bellingham + Stanley Ltd. E-Line 90 User manual

The E-Line 90 is a full scale Brix refractometer ideal for use in the food
industry and other applications requiring concentration measurements
over a wide measurement range.
Applying the sample to the refractometer:
Lift the illuminating prism1and apply the sample to the measurement
prism. Close the prism and view the borderline (light/dark
demarcation line) through the eyepiece2.
Taking a reading:
Depending on the concentration of the sample, select the range of
measurement(Table 1) by adjusting the scale selection control3. If the
concentration is unknown, apply the sample and step through the
ranges until a clear borderline is found. Adjustment of the dispersion
ring4 will sharpen the borderline and should be set so that no colour
(blue/red) can be seen near the demarcation line. For dark samples,
adjust the cover plate5to allow more light to enter the prism.
The concentration of the sample can be measured at the point the
borderline crosses the scale. Take care to ensure that the reading is
taken from the correct scale range.
Adjust the reading by measuring the temperature6of the instrument
and adding the correction factor as shown on Table 2. Refractive
Index values can be obtained by using a conversion table available
from Bellingham + Stanley Ltd.
Calibration:
Water may be used for checking the zero of the instrument, whilst
accurately made sugar solutions are ideal for checking points further
up the scale. When using water or sugar standards, always apply
temperature correction (Table 2). Solid test plates may also be used
but do not require temperature correction. The E-Line 90 does not
have a facility to adjust calibration and in the event that the calibration
is found to be incorrect, please contact Bellingham + Stanley Ltd. For
further information on calibration materials, visit the Bellingham
+ Stanley website.
Always check sample Health & Safety Data before applying to the
refractometer.
When applying samples to the prism which are likely to cause harm to
skin or eyes, wear appropriate protective clothing and glasses.
This refractometer is a precision optical instrument and should be
handled with care. Do not drop or subject it to sharp knocks.
Optical glass is relatively soft and care should be taken not to scratch
the prism surface. Do not use metal spatulas or glass rods to apply
samples but instead use softer materials such as plastic.
Table 1
1 Illuminating
Prism
B
ellingham + Stanley Ltd.
Longfield Road, Tunbridge Wells, Kent TN2 3EY, United Kingdom.
Telephone: [phone] Fax: [phone]
Website: www.bellinghamandstanley.com Email: [e-mail]
6 Thermometer
2 Eyepiece
5 Cover Plate
3 Scale Selection
Control
4Disperson Rin
g
Specification
Range
Scale One
Scale Two
Scale Three
0-90 ˚Brix
0-42 ˚Brix
42-71 ˚Brix
71-90 ˚Brix
Minimum Division 0.2 ˚Brix
E-Line 90 Refractometer
Instruction Manual
E-Line Refractometer top side
E-Line Refractometer under side
Warning
Order Code Description
44-806 E-Line 90 Refractometer
44-892 Iss 01 Rev 0

B
ellingham + Stanley Ltd.
Longfield Road, Tunbridge Wells, Kent TN2 3EY, United Kingdom.
Telephone: [phone] Fax: [phone]
Website: www.bellinghamandstanley.com Email: [e-mail]
E-Line 90 Refractometer
Instruction Manual
Correcting the reading for temperature:
The refractometer is calibrated to read correctly at 20˚C. The refractive index of the measured sample will vary with temperature and the
reading must be corrected to take account of this. Please use the Table 2 below to adjust your reading accordingly.
Temperature Correction Table (Table 2)
This table gives mass fraction corrections to refractometric tables for sucrose solutions at 589nm for temperature different from 20˚C. Taken
from ICUMSA, Appendix 2, SPS-3 (1998) page 8.
Temperature (°C) Measured Sucrose ˚Brix (Mass Fraction)
0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85
15 -0.29 -0.30 -0.32 -0.33 -0.34 -0.35 -0.36 -0.37 -0.37 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.38 -0.37 -0.37
16 -0.24 -0.25 -0.26 -0.27 -0.28 -0.28 -0.29 -0.30 -0.30 -0.30 -0.31 -0.31 -0.31 -0.31 -0.31 -0.30 -0.30 -0.30
17 -0.18 -0.19 -0.20 -0.20 -0.21 -0.21 -0.22 -0.22 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.23 -0.22
18 -0.12 -0.13 -0.13 -0.14 -0.14 -0.14 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15 -0.15
19 -0.06 -0.06 -0.07 -0.07 -0.07 -0.07 -0.07 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.08 -0.07
20 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00
21 0.06 0.07 0.07 0.07 0.07 0.07 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.08 0.07
22 0.13 0.14 0.14 0.14 0.15 0.15 0.15 0.15 0.16 0.16 0.16 0.16 0.16 0.16 0.15 0.15 0.15 0.15
23 0.20 0.21 0.21 0.22 0.22 0.23 0.23 0.23 0.23 0.24 0.24 0.24 0.24 0.23 0.23 0.23 0.23 0.22
24 0.27 0.28 0.29 0.29 0.30 0.30 0.31 0.31 0.31 0.32 0.32 0.32 0.32 0.31 0.31 0.31 0.30 0.30
25 0.34 0.35 0.36 0.37 0.38 0.38 0.39 0.39 0.40 0.40 0.40 0.40 0.40 0.39 0.39 0.38 0.38 0.37
26 0.42 0.43 0.44 0.45 0.46 0.46 0.47 0.47 0.48 0.48 0.48 0.48 0.48 0.47 0.47 0.46 0.46 0.45
27 0.50 0.51 0.52 0.53 0.54 0.55 0.55 0.56 0.56 0.56 0.56 0.56 0.56 0.55 0.55 0.54 0.53 0.52
28 0.58 0.59 0.60 0.61 0.62 0.63 0.64 0.64 0.64 0.65 0.65 0.64 0.64 0.63 0.63 0.62 0.61 0.60
29 0.66 0.67 0.68 0.70 0.71 0.71 0.72 0.73 0.73 0.73 0.73 0.73 0.72 0.72 0.71 0.70 0.69 0.67
30 0.74 0.76 0.77 0.78 0.79 0.80 0.81 0.81 0.82 0.82 0.81 0.81 0.80 0.80 0.79 0.78 0.76 0.75
31 0.83 0.84 0.85 0.87 0.88 0.89 0.89 0.90 0.90 0.90 0.90 0.89 0.89 0.88 0.87 0.86 0.84 0.82
32 0.92 0.93 0.94 0.96 0.97 0.98 0.98 0.99 0.99 0.99 0.99 0.98 0.97 0.96 0.95 0.93 0.92 0.90
33 1.01 1.02 1.03 1.05 1.06 1.07 1.07 1.08 1.08 1.08 1.07 1.07 1.06 1.04 1.03 1.01 1.00 0.98
34 1.10 1.11 1.13 1.14 1.15 1.16 1.16 1.17 1.17 1.16 1.16 1.15 1.14 1.13 1.11 1.09 1.07 1.05
35 1.19 1.21 1.22 1.23 1.24 1.25 1.25 1.26 1.26 1.25 1.25 1.24 1.23 1.21 1.19 1.17 1.15 1.13
36 1.29 1.30 1.31 1.33 1.34 1.34 1.35 1.35 1.35 1.34 1.34 1.33 1.31 1.29 1.28 1.25 1.23 1.20
37 1.39 1.40 1.41 1.42 1.43 1.44 1.44 1.44 1.44 1.43 1.43 1.41 1.40 1.38 1.36 1.33 1.31 1.28
38 1.49 1.50 1.51 1.52 1.53 1.53 1.54 1.54 1.53 1.53 1.52 1.50 1.48 1.46 1.44 1.42 1.39 1.36
39 1.59 1.60 1.61 1.62 1.63 1.63 1.63 1.63 1.63 1.62 1.61 1.59 1.57 1.55 1.52 1.50 1.47 1.43
40 1.69 1.70 1.71 1.72 1.73 1.73 1.73 1.73 1.72 1.71 1.70 1.68 1.66 1.63 1.61 1.58 1.54 1.51
Cleaning the prism:
Thoroughly clean the prism after use with water or other suitable solvent and dry with clean tissue.
The prism surface could be damaged by strong alkalis or acids if left in contact for long periods of time. Clean samples from the
prism as soon as practicable.
Wiping the prism surface occasionally with alcohol will remove any build-up of oils left from the samples.
44-892 Iss 01 Rev
0