BioFluidix Tek-Matic PipeJet NanoDispenser User manual

PipeJet®
NanoDispenser Kit
HARDWARE MANUAL
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Hardware manual
PipeJet®NanoDispenser Kit
English
2
Copyright 2021 BioFluidix GmbH, Freiburg.
No part of this document may be reproduced without prior permission of the copyright owner.
Document Version: 2.4
Publication Date: 22.07.2022
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Hardware manual
PipeJet®NanoDispenser Kit
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Preface
Contact Us
Please contact Tek-Matic if you have questions about a product that are not answered in
the manual. Be prepared and provide the following information:
•Product serial number
•Software version
•Error number, if available
Product service plan, support, and warranty extension
BioFluidix offers several service and warranty programs to keep your system running.
Get in touch and get more information about our services:
•Preventative Maintenance (PM)
•Technical support, remote or on-site
•Application and process development
•Software update
•Extended warranty
Product hands on training
Call BioFluidix if you are new to the device and want an interactive product training on-site or
via remote. An application specialist will explain all software functions and answer your
questions.
Warranty and Product Liability
The PipeJet®NanoDispenser Kit comes with a 12-month warranty upon delivery. The warranty
and product liability do not apply, in case of improper handling of the device, misuse, disregard
of the intended use or if the system was opened. Wear and tear parts are excluded from
warranty.
Tek-Matic
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Hardware manual
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Table of content
1User instructions .................................................................................................................................................... 5
1.1 Using this manual................................................................................................................................................... 5
1.2 Intended use............................................................................................................................................................ 5
1.3 Foreseeable misuse ............................................................................................................................................... 5
2Safety........................................................................................................................................................................ 6
2.1 Safety Instructions ................................................................................................................................................. 6
2.2 Warnings and safety signs.................................................................................................................................... 7
3Product description ............................................................................................................................................... 8
3.1 Features ................................................................................................................................................................... 8
3.2 Product Overview.................................................................................................................................................. 8
3.3 List of Parts.............................................................................................................................................................. 9
3.4 Hardware description..........................................................................................................................................10
3.4.1 PipeJet® Nanodispenser ......................................................................................................................................10
3.4.2 Dispensing pipes ...................................................................................................................................................10
3.4.3 BioFluidix Control ElectroniX 200: Front Panel .............................................................................................11
3.4.4 BioFluidix Control ElectroniX 200: Connection Panel ..................................................................................12
4Installation and operation...................................................................................................................................13
4.1 Connecting the parts ...........................................................................................................................................13
4.2 Installation of the dispensing pipes ..................................................................................................................14
4.3 Priming the PipeJet®Nanodispenser...............................................................................................................16
4.3.1 Sample volume up to 1ml....................................................................................................................................16
4.3.2 Sample volume < 50 µl ........................................................................................................................................17
5Maintenance, Cleaning, Storage, Disposal ......................................................................................................17
5.1 Maintenance .........................................................................................................................................................17
5.2 Cleaning the consumables..................................................................................................................................17
5.3 Storage ...................................................................................................................................................................18
5.4 Disposal..................................................................................................................................................................18
6Technical details ...................................................................................................................................................18
6.1 Technical and Environmental Specifications ..................................................................................................18
6.1.1 Power supply .........................................................................................................................................................18
6.1.2 Weight/dimensions Control ElectroniX ...........................................................................................................18
6.1.3 Ambient conditions ..............................................................................................................................................18
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6.2 Basic Settings and Details Control ElectroniX 200 .......................................................................................18
6.2.1 Basic Settings of the BioFluidix Control ElectroniX 200 ..............................................................................18
6.2.2 Connectors of Control ElectroniX 200.............................................................................................................19
6.3 Ordering information...........................................................................................................................................20
6.3.1 Dispensing units and Control ElectroniX .........................................................................................................20
6.3.2 Dispensing pipes and accessories......................................................................................................................20
7Troubleshooting ...................................................................................................................................................21
1 User instructions
1.1 Using this manual
Read the user manual completely before first use and keep this manual for future reference. You
will find detailed information regarding Care and Use and Contact information for our Customer
Service.
1.2 Intended use
The PipeJet®NanoDispenser Kit is intended for the following uses:
•Single channel dispensing of liquids for scientific experiments or for integration in an
automation system.
•Dispensing of liquids in a viscosity range of 0.5 –100 mPas with a single droplet volume in
the range of 2–70 nl* and a maximum dispensing frequency of 100 Hz*. (*For DI-water at
RT)
•The PipeJet®NanoDispenser Kit is only intended for indoor use in a laboratory or industrial
environment.
•Only dispense liquids approved by BioFluidix. Please contact BioFluidix for further
information. Please consider that you can harm yourself or damage the PipeJet®
NanoDispenser Kit by applying non-approved liquid types.
Another use as listed is not allowed and will lead to damage of the PipeJet®NanoDispenser Kit.
The whole product may not be changed or reconstructed.
1.3 Foreseeable misuse
•Use of a non-proofed medium, a medium hazardous to health, harmful to the environment
medium or explosive medium.
•Use of the dispenser with an unauthorized control electronic.
•Use of the BioFluidix Control ElectroniX 200 for other purposes than driving the PipeJet®
SiJet or ValveJet dispensers.
•Changing the original PipeJet®calibration by altering the position of the calibration screw.
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2 Safety
2.1 Safety Instructions
Safe operation of the device is only ensured if the recommendations written in this manual or
displayed on the device or in the software are followed.
•Avoid operation in contrarious environmental conditions. This may lead to damage to the
electronics and to a possible hazard for the health of the user.
Contrarious environmental conditions are:
•Too high humidity (greater than 80%, condensing)
•Moistness
•Too high environmental temperature (more than 45°C)
•Only put the BioFluidix Control ElectroniX 200 in operation if the chassis is safety closed
with inserted nuts and there are no visible damages like isolation damages on cables.
•Only use parts provided within the shipment or order parts from BioFluidix/ your local
supplier.
•Shut down the device in a potentially unsafe operation.
•If it is supposed that it is not possible to launch a safe operation, you have to take it out of
service and protect it against an unintended operation. It is supposed that a safe operation
is not possible if
•the kit shows visible damages,
•the kit isn´t working and
•after a longer storage in bad conditions or
•after heavy load due to transport.
•Never connect the Control ElectroniX 200 to the electrical grid if it comes from a cold room
in a warmer one. The resulting water condensation can damage the electronic. Always let
the unconnected BioFluidix Control ElectroniX 200 warm up to the room temperature.
•Do not immerse the dispenser into liquid! Keep dispenser dry at all times! If the dispenser
gets into contact with liquids, disconnect it from voltage supply and make sure it has dried
completely before next use. In doubt contact BioFluidix for further assistance.
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2.2 Warnings and safety signs
Warning - High voltage hazard!
Do not open the dispenser or the BioFluidix Control ElectroniX 200! The device
contains circuits which carry power supply voltage. Service and maintenance of the
PipeJet®NanoDispenser Kit is exclusively executed by BioFluidix personnel.
Warning - High voltage hazard after the device has been switched off!
After switching off the device, a DC voltage of 150 V remains in the circuits for several
minutes due to a capacitance in the circuits!
Warning - Danger of fire, explosion, and injury by use of aggressive liquids!
Do not use or clean the device with flammable or chemical aggressive media or
solvents. Always wear protective gloves, eye protection and lab coats when operating
the device. Always consult the safety data sheets of the liquids used for dispensing and
ensure that the required safety measures are taken.
Warning - Electric shock from damage to device or power cable
Only switch on the device if all electrical cables are undamaged. Only use a properly
installed and repaired device.
NOTICE
This product can only be operated by qualified staff or trained personnel. Other
uses different from those listed above are not allowed and will lead to damage
of the PipeJet®NanoDispenser Kit. The whole product may not be changed or
reconstructed.
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3Product description
3.1 Features
The PipeJet®Nanodispenser Kit combines all essentials to get started with the first dispensing
trials right after installation. It never was so easy: unbox all items, install the software, connect
the control unit, insert a pipe, fill in your liquid and start with the dispensing.
3.2 Product Overview
Figure 1: PipeJet®NanoDispenser Kit –Product Overview
1
Torque Handle
5
Pipe Guide Set LV
for Pipes ID125 (Front = Black; Back = Red)
2
PipeJet®Pipe Handling Tool
6
Pipe Guide Set HV
for Pipes ID200 & ID500 (Front = Red; Back =
Black)
3
Control ElectroniX 200
7
PipeJet® NanoDispenser
USB cable
8
USB Stick incl. Control Software
4
Power supply
9
PipeJet®Pipes Type 125-C
Volume range: 1 –7 nL
Disposable reservoir (1ml)
Laboratory tubing
10
PipeJet®Pipes Type 200
Volume range: 6 –20 nL
Tool Set
PipeJet®Connection Cable
11
PipeJet®Pipes Type 500
Volume range: 20 –70 nL
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3.3 List of Parts
Please make sure that the following items are included in your PipeJet® NanoDispenser Kit. If
any items are missing, please contact BioFluidix.
1x PipeJet®
NanoDispenser
1x PipeJet®
Pipe Handling Tool
1x BioFluidix Control ElectroniX 200
and power supply
1x Pipe Guide Set HV for
Pipes Types 200 / 500
1x Pipe Guide Set LV for
Pipe Types 125
10x PipeJet®Pipes Type 125-C
10x PipeJet®Pipes Type 200
10x PipeJet®Pipes Type 500
1x Reservoir Holder
10x 1 ml Reservoir
1 m Laboratory Tubing
1x Torque Handle for
fixation of the
Pipe Guides
1x PipeJet®Connection
Cable
1x USB cable
1x M4x16
1x Allen key (2.5mm)
1x BioFluidix USB Stick
incl. Control Software
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3.4 Hardware description
3.4.1 PipeJet® Nanodispenser
The PipeJet® Nanodispenser is a piezo-actuated dispensing unit based on a direct volume
displacement. The module is designed for the non-contact dispensing of single droplets in the
range from 2 nl to 70 nl. Different pipe types are available to address different volume ranges.
The dispensing pipe types vary in diameter, length, and coating to meet the requirements of
various applications.
Figure 2: Side view of a PipeJet® Nanodispenser
1
Dispensing pipe
3
Fixation screws
2
Pipe Guide (front and rear)
4
Positioning pins (pre-installed if
ordered)
3.4.2 Dispensing pipes
The dispensing pipes are intended for the use with a PipeJet®Nanodispenser. Different types of
dispensing pipes are available varying in size. and coating. The following pipes come with the Kit:
Table 1: Description of dispensing pipes
Pipe type
Size
Volume
125-C
Short
2 –7 nL*
200
Short
6 –20 nL*
500
Short
20 –70 nL*
* Volume ranges refer to dispensing DI-water at RT.
Calibration screw!
The calibration screw must not be used by the customer. The dispense modules are
calibrated and tested before shipment. Working on the calibration screw will exclude
reproducible dispensing performance.
1
2
3
4
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!
Material damage caused by incorrect handling and use.
Do not twist the pipes or change positioning after attachment to the pipe guide.
!
Do not reuse after dismounting.
The pipes are single-use products. The materials are not qualified for multiple mounting.
!
Prevent nozzle orifice damage.
Only remove the pipes from packaging in the ‘free space’ area without touching the packaging
wall. Read the operation instructions for more detailed information.
Top view:
Figure 3: Dispensing pipes in original packaging. The dispensing pipes should be guided to the ‘free space’ area to
be unpacked safely from packaging without touching the wall.
3.4.3 BioFluidix Control ElectroniX 200: Front Panel
Warning - The housing of the BioFluidix Control ElectroniX 200 is floating
It can be connected to an existing potential by connecting the grounding screw.
‘Free
space’
‘Free
space
area’
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Figure 4: Control ElectroniX 200 - Front Panel
1
Power LED: lights up when Control
ElectroniX 200 is switched on
4
Dispenser Connector (12x): Connection
for up to 12 dispensing units
2
Active LED: flashes during dispensing event
5
Channel LED (12x): lights if respective
dispensing channel is activated
3
Start Button: initiates dispensing event
3.4.4 BioFluidix Control ElectroniX 200: Connection Panel
Figure 5: Control ElectroniX 200 - Connection Panel (see Chapter 0 for detailed description)
1
Power input (for provided AC Adaptor)
6
LED: Connector for SmartDrop System
2
RS-232: serial connector
7
USB connector
3
Power input
8
Trigger (In and Out)
4
Grounding screw
9
Power toggle switch
5
Connector for valves
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4 Installation and operation
4.1 Connecting the parts
The installation by a trained and authorized personnel is recommended for a safe initial start-up
and proper function of the device. Please contact your supplier or BioFluidix for an installation
service and/or hand-on training.
•Connect the 24 V plug into the BioFluidix Control ElectroniX 200 then connect the wall
power supply to an approved plug socket.
•Start the BioFluidix Control ElectroniX 200 using the toggle switch.
•After a flash of the red, then the orange LED at the Front panel, the BioFluidix Control
ElectroniX 200 is in standby, indicated by illumination of the green Power LED only.
•Connect the dispenser by using the included PipeJet®Connection Cable with the BioFluidix
Control ElectroniX 200.
•Install the PipeJet® NanoDispenser on the desired location (e.g. a fixation bar, post or
similar) using the mounting holes and provided screws.
•Connect the BioFluidix Control ElectroniX 200 with a control Computer/Laptop using the
provided USB-cable.
•Follow the installation instructions provided on the USB-Stick to install the BioFluidiX
Control Software.
•Consult the BioFluidiX Control Software Manual and start the Software.
•If encounter any problems, check the Troubleshooting Chapter 0. Should your specific
problem still encounter please contact your supplier or BioFluidix.
Connect to standard power supply socket with 90 V –240 V and fuse >= 6 A, B –C
characteristic.
Only use the power chord supplied.
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4.2 Installation of the dispensing pipes
1
Reservoir (e.g. pipette tip)
2
Connection tube (Tygon, approx. 80mm piece)
3
PipeJet pipe
4
pipe guide screw
5
PipeJet body
6
Pipe guide back
7
Pipe guide front
8
Reservoir holder (not shown, can be attached to the
M4 thread, see next image)
1
Install the reservoir holder on the side of the
PipeJet® Nanodispenser using the provided
screw and tool.
2
Prepare the PipeJet® Nanodispenser with the rear
pipe guide.
1
Insert the pipette tip reservoir (1) into a approx.
80 mm piece of connection tube (2).
2
Connect a dispensing pipe to the tube setup.
1
2
3
4
5
6
7
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3
Remove the dispensing pipe as described in
chapter 3.4.2 Use the ‘free space’ area.
4
Insert the dispensing pipe into the rear pipe guide.
5
Close the PipeJet® NanoDispenser via the front
pipe guide.
6
Connect the reservoir to the reservoir holder.
7
Fix the dispensing pipe with 2x screws and
correct torque via the provided torque handle.
Make sure that the Pipe is positioned correctly in the centered rhombic
opening of the guide bottom!
Take special care not to bend or twist the PipeJet® Pipe when connecting the
dispensing tube and handling the reservoir.
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4.3 Priming the PipeJet®Nanodispenser
4.3.1 Sample volume up to 1ml
•Fill the liquid into the liquid reservoir. The liquid
will rise in the tubing without contacting the
PipeJet® Pipe as shown on the picture.
•Take the reservoir out of the holder and lift it
carefully until the liquid in the tubing contacts
the PipeJet®Pipe and a small droplet appears at
the nozzle.
•Lower the reservoir until the droplet at the Pipe
nozzle is drawn back and put the reservoir back
in the reservoir holder.
•If required soak up pending droplets at the
nozzle with a piece of wipe (without contacting
the PipeJet®Pipe).
•Take care that the tubing is filled without any air
bubbles.
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4.3.2 Sample volume < 50 µl
•Cut off a small piece of the laboratory tubing.
•Connect the laboratory tubing with the
PipeJet®Pipe.
•Fill the laboratory tubing with a pipette until a
small droplet appears at the nozzle. Make sure
that no bubbles are trapped.
•Carefully remove the droplet and if required
soak up the droplet at the nozzle with a piece
of wipe (without contacting the PipeJet®Pipe).
5 Maintenance, Cleaning, Storage, Disposal
5.1 Maintenance
The PipeJet® NanoDispenser Kit is a maintenance free system. If parts of the system will be
opened or demount, the warranty claim will expire immediately. For surface cleaning, take a
clean, dry, antistatic, lint-free cleaning tissue.
5.2 Cleaning the consumables
The dispensing pipes are intended for single use only. It is recommended to dispose the
consumables after use to prevent cross contamination or material damage by cleaning procedure
and agent.
Pipe cleaning
BioFluidix does not guarantee the dispensing performance for manually cleaned pipes.
Damage to the pipe during manual cleaning is at high probability. Certain cleaning
solutions can change the surface energy of the pipes which leads to different
dispensing behavior, non-proper capillary filling or wetting of the pipes.
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5.3 Storage
If you intend to leave the device inactive for a longer period, observe the storage conditions.
Clean the PipeJet®NanoDispenser Kit with all components and store the device in the original
packaging.
5.4 Disposal
Dispose the PipeJet®NanoDispenser Kit conform to the guilty law. Take special care of chemical
waste resulting during the operation of the kit. Please contact your person in charge for special
waste in your company for a safety removal.
6 Technical details
6.1 Technical and Environmental Specifications
6.1.1 Power supply
Voltage
90 V –240 V, AC
Frequency
50 Hz –60 Hz
Maximum power consumption
60 W
6.1.2 Weight/dimensions Control ElectroniX
Weight
1850 g
Width
Depth
Height
210 mm
255 mm
80 mm
6.1.3 Ambient conditions
Operation condition
+ 10 °C to + 45 °C
10 % to 80 % rel. humidity, non-condensing
Storage conditions
- 10 °C to + 50 °C
5 % to 95 % rel. humidity, non-condensing
6.2 Basic Settings and Details Control ElectroniX 200
6.2.1 Basic Settings of the BioFluidix Control ElectroniX 200
LoadCurrent
500 mA
Offlevel
42.7 mV
UnloadCurrent
5 mA
DetectLevel
12.2 mV
LoadTime
200 µs
Shots
1
HoldTime
100 µs
Shot Delay
100 ms
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6.2.2 Connectors of Control ElectroniX 200
RS-232 serial connector:
To connect the BioFluidix Control ElectroniX 200 to the computer, a 9-pin to 9-pin null modem
cable is needed. The RS232 settings are:
•Bits per second: 115200 baud
•Data bits: 8
•Parity: None
•Stop bits: 1
•Flow control (Handshaking): None
•CR, LF
Power Input:
24VDC power supply input. NOTE: use only one of the power input ports at the same time
Grounding screw:
Intended to earth the case of the device
Connector for valves:
Two 24V switchable connectors for connecting additional valves
LED:
Connector for SmartDrop Camera System
USB-connector
Intended to connect the electronics to the PC
Trigger In:
The input trigger can be used to initiate a dispensing event externally by a 3.3V LVTTL or a 5V
TTL signal for example to enable a synchronous operation with positioning facilities or in
automated measuring rigs.
Trigger Out:
The output trigger can be used to synchronise external devices with the PipeJet®
NanoDispenser (for example a camera or other measuring devices). With every dispensing event
an output pulse of 3.3V is generated.
Power toggle switch:
Enables / Disables the power supply of the Control ElectroniX CX200
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6.3 Ordering information
6.3.1 Dispensing units and Control ElectroniX
Order no.
Description
PJ-30091
PipeJet®NanoDispenser (single channel)
Volume ranges from 2 nl –70 nl
CX200-1800 E
ControlElectroniX CX200
Control Electronic for up to 12 dispensers
6.3.2 Dispensing pipes and accessories
Order no.
Description
PJ-12511
Dispensing pipes 125-S-C
Coated, 23mm (total length), 2mm (free pipe length), 1 box = 16 pipes
PJ-20010
Dispensing pipes 200-S
23mm (total length), 2mm (free pipe length), 1 box = 16 pipes
PJ-20011
Dispensing pipes 200-S-C
Coated, 23mm (total length), 2mm (free pipe length), 1 box = 16 pipes
PJ-20050
Dispensing pipes 200-L
28mm (total length), 7mm (free pipe length), 1 box = 16 pipes
PJ-20051
Dispensing pipes 200-L-C
Coated, 28mm (total length), 7mm (free pipe length), 1 box = 16 pipes
PJ-50070
Dispensing pipes 500-S
23mm (total length), 2mm (free pipe length), 1 box = 16 pipes
PJ-50071
Dispensing pipes 500-S-C
Coated, 23mm (total length), 2mm (free pipe length), 1 box = 16 pipes
PJ-50080
Dispensing pipes 500-L
28mm (total length), 7mm (free pipe length), 1 box = 16 pipes
PJ-50081
Dispensing pipes 500-L-C
Coated, 28mm (total length), 7mm (free pipe length), 1 box = 16 pipes
VM-20053
Tygon –LMT-55
Fluidic tubing. 1 PU = 1 meter
VM-20101
Disposable Reservoir
1000µl, 1 PU = 10 Pcs.
PJ‐50000
PipeJet reservoir holder
For 1000 μl reservoir size
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