CORNING Axygen Axypet User manual

A Corning Brand
Axygen® Axypet®
Pipettors
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual

Table of Contents
1. Product Description ..............................................................1
2. Setting the Volume................................................................5
3. Aspiration and Dispensing Instructions........................... 8
4. Pre-rinsing................................................................................ 9
5. Dense and Viscous Liquids.................................................10
6. Axygen® Pipet Tips...............................................................10
7. Recommendations...............................................................10
8. Recalibration .........................................................................11
9. Troubleshooting ...................................................................15
10. Cleaning and Sterilization..................................................17
11. Components..........................................................................18
12. Spare Parts .............................................................................18
13. Limited Warranty .................................................................19

Instruction Manual | 1
1. Product Description
The Axygen® Axypet® single-channel pipettor is a
volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 0.1 μL to 10,000 μL depending on the model.
Axygen Axypet 8-channel and 12-channel pipettors have
been designed for microplate filling. The pipettors enable
precise and simultaneous delivery of 8 or 12 preset volume
doses of liquid. These pipettors are produced in four volume
ranges: 0.5 to 10 μL, 5 to 50 μL, 20 to 200 μL, 50 to 300 μL.
Model Volume Range (μL) Application
2, 10
Measurement and transfer of
micro-volumes, DNA sequencing,
and enzyme assay applications.
20, 50, 100, 200,
250, 300, 1,000
Measurement and transfer of general
aqueous solution, acids, and bases.
5,000,
10,000
Measurement and transfer of large
volumes.
The single-, 8-, and 12-channel pipettors feature a digital
counter which shows the pipetting volume. The set
volume is visible in the window on the handle. Volumes
are set by either turning the pipetting pushbutton (A1) or
the adjustment knob (B) in the correct direction.
A1. Pushbutton
A2. Knob
B. Adjustment knob
P. Identication ring
C. Shaft
D. Ejector
E. Tip

2 | AXYGEN AXYPET PIPETTORS
The pipettor design allows the user to lock the volume
setting by sliding the locking ring upwards.
The symbols printed on the handle show locked and
unlocked positions. The volume range of each pipettor
model is shown on the pipetting pushbutton (A1).
Liquids are aspirated into disposable pipet tips attached
to the pipettor shaft. Disposable pipet tips ensure
maximum safety and eliminate the possibility of cross-
contamination of the liquid samples. To protect the user
from contaminated pipet tips, the pipettor is fitted with a
built-in tip ejector (D).
Single-channel Pipettor Ejector
The ejector length can be adjusted to accommodate all
common brands of pipet tips. When using narrow tubes,
it may be necessary to remove the tip ejector. This is done
simply by pulling down.
Adjusting the Tip Ejector Length
2 to 1,000 μL Pipettors
The “H“ spacers included with
each pipettor, allow the ejector
length to be adjusted by +1 mm
or +2 mm.
An ”H0” spacer is fitted as
standard. The outside shape
of the spacer identifies the
adjustment.
SS

Instruction Manual | 3
5,000 and 10,000 μL Pipettors
The length of tip ejector is adjusted by screwing in or
screwing out the tip ejector arbor with a small screwdriver.
Turn the screwdriver counterclockwise to increase the length
of the tip ejector, and clockwise to reduce the length of the
tip ejector. The ejector can be adjusted by up to 5 mm.
If the ejector adjustment described above is not sufficient
or the diameter of the ejector opening is too large to eject
the tip, it will be necessary to put the ejector cap “M” onto
the ejector.
2 and 10 μL Pipettors
The 2 μL to 10 μL pipettors may require the use of an
ejector cap “M” to efficiently eject certain brands of pipet
tips. Simply place the cap (supplied with the pipettor)
on the bottom of the pipettor shaft and slide the cap
upwards until it surrounds the bottom of the tip ejector.
L
MM
5,000 μL: L + 6 mm
10,000 μL: L + 10 mm

4 | AXYGEN AXYPET PIPETTORS
8-channel and 12-channel Pipettor Ejector
The 8-channel and 12-channel pipettor construction
allows the lower 8-channel and 12-channel module to be
rotated 360°. This allows the user to disable the ejector to
avoid accidental use and to change its position to make
filling of microplates more comfortable and convenient. It
is recommended to turn the module clockwise.
The pipettor is a high quality instrument which offers
excellent accuracy and precision.
The accuracy and precision (repeatability) of the liquid
volume depends on the quality of pipet tips used. The
values for accuracy and precision shown in the table below
were obtained using Axygen® pipet tips.
Model
Volume
Range
(μL) Cat. No.
Volume
(μL)
Accuracy
(%)
Precision
(%)
Fit to Tips
Without
Filter
(μL)
Single-channel Pipettors
2 AP-2
Min. 0.1 ±40.0 ≤12.0
101 ±2.7 ≤1.30
Max. 2 ±1.5 ≤0.70
10 AP-10
Min. 0.5 ±4.0 ≤2.80
105 ±1.0 ≤0.60
Max. 10 ±0.5 ≤0.40
20 AP-20
Min. 2 ±3.0 ≤1.50
20010 ±1.0 ≤0.50
Max. 20 ±0.8 ≤0.30
50 AP-50
Min. 5 ±2.5 ≤2.00
200
25 ±1.0 ≤0.60
Max. 50 ±0.8 ≤0.40
100 AP-100
Min. 10 ±1.6 ≤0.80
20050 ±0.8 ≤0.24
Max. 100 ±0.8 ≤0.20
200 AP-200
Min. 20 ±1.2 ≤0.60
200100 ±0.8 ≤0.25
Max. 200 ±0.6 ≤0.20
250 AP-250
Min. 50 ±1.0 ≤0.40
300125 ±0.8 ≤0.30
Max. 250 ±0.6 ≤0.30
1,000 AP-1000
Min. 100 ±1.6 ≤0.40
1,000500 ±0.7 ≤0.20
Max. 1,000 ±0.6 ≤0.15

Instruction Manual | 5
Model
Volume
Range
(μL) Cat. No.
Volume
(μL)
Accuracy
(%)
Precision
(%)
Fit to Tips
Without
Filter
(μL)
Single-channel Pipettors (continued)
5,000 AP-5000
Min. 1,000 ±0.6 ≤0.25
5,0002,500 ±0.6 ≤0.20
Max. 5,000 ±0.5 ≤0.15
10,000 AP-10ML
Min. 1,000 ±2.5 ≤0.60
10,0005,000 ±0.8 ≤0.30
Max. 10,000 ±0.5 ≤0.20
8-channel and 12-channel Pipettors
8 - 10
12 - 10
AP-8-10
AP-12-10
Min. 0.5 ±10.0 ≤8.0
105 ±4.0 ≤2.0
Max. 10 ±2.0 ≤1.2
8 - 50
12 - 50
AP-8-50
AP-12-50
Min. 5 ±4.0 ≤2.5
20025 ±3.0 ≤1.2
Max. 50 ±1.6 ≤0.6
8 - 200
12 - 200
AP-8-200
AP-12-200
Min. 20 ±3.0 ≤1.5
200100 ±1.5 ≤0.8
Max. 200 ±1.0 ≤0.6
8 - 300
12 - 300
AP-8-300
AP-12-300
Min. 50 ±1.6 ≤1.5
300150 ±1.2 ≤1.0
Max. 300 ±1.0 ≤0.6
The accuracy and precision are obtained gravimetrically using
Axygen® pipet tips, performing at least 10 measurements
of distilled water at a reference temperature of 20°C ± 1°C
according to EN ISO 8655 standard.
2. Setting the Volume
The volume of the pipettor is set by turning the pipetting
pushbutton (A2) or the black adjustment knob (B).
The volume display consists of three numbers and is
read from top to bottom. The three numbers indicate the
volume selected and are colored black and red.
Red numbers represent decimal fractions for microvolume
pipettors or milliliters for macrovolume pipettors.

6 | AXYGEN AXYPET PIPETTORS
An example for each of these pipettors is given below.
Model Volume
Range (μL)
Counter
Readings
Set
Volume Increment
Single-channel Pipettors
2
1
2
5
1.25 μL 0.002 μL
10
0
7
5
7.5 μL 0.02 μL
20
1
2
5
12.5 μL 0.02 μL
50
0
3
8
38.0 μL 0.2 μL
100
0
7
5
75.0 μL 0.2 μL
200
1
2
5
125.0 μL 0.2 μL
250
2
4
5
245.0 μL 0.2 μL
1,000
0
7
5
750.0 μL 2 μL
5,000
1
2
5
1.25 mL 10 μL
10,000
0
7
5
7.5 mL 20 μL

Instruction Manual | 7
Model Volume
Range (μL)
Counter
Readings
Set
Volume Increment
8-channel and 12-channel Pipettors
8 - 10
12 - 10
0
3
5
3.5 μL 0.02 μL
8 - 50
12 - 50
0
6
5
6.5 μL 0.10 μL
8 - 200
12 - 200
0
8
5
85.0 μL 0.20 μL
8 - 300
12 - 300
2
5
0
250.0 μL 1.0 μL
To attain the maximum accuracy, the set volume must be
approached from a higher value.
If the desired value is lower than the previous value, adjust
the value down to a one-third turn above the required
setting. If the desired value is higher than the previous
value, adjust the value to a one-third turn above the
required value. The setting should then be performed in
the following manner:
• Slowly turn the pipetting pushbutton or the black
adjustment knob to the required volume.
• If the knob is accidentally turned too far below the
required value, the process must be repeated.
• Set the locking ring into the upper position following
the volume adjustment, thus locking the knob and
preventing accidental volume change.

8 | AXYGEN AXYPET PIPETTORS
3. Aspiration and Dispensing Instructions
Single-channel Pipettors
Place a pipet tip on the shaft of the pipettor. See Section 6
for the appropriate tip. Press the pipet tip on firmly using a
slight twisting motion. This will ensure an airtight seal.
8-channel and 12-channel Pipettors
To put pipet tips on the shafts: hold the pipettor vertically
and press it against the tips in the rack box until the shafts
retract approximately 1.5 mm into the manifold. The
suspension system of the shafts ensures even and tight
sealing of the pipet tips. The rocking movement does not
have to be performed to seal the pipet tips tightly.
NOTE: Never handle a liquid with a pipettor which has not
been fitted with a pipet tip.
Aspiration
1. Press the pushbutton to the first positive stop. Holding
the pipettor vertically, immerse the pipet tip into the
sample liquid. The depth to which the pipet tip is
immersed in the sample liquid depends on the model.
Model Volume Range (μL) Immersion Depth (mm)
2, 10 ≤1
20, 50, 100 2 - 3
200, 250, 300, 1,000 2 - 4
5,000 3 - 6
10,000 5 - 7
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