CORNING Axygen Axypet User manual

A Corning Brand
Axygen® Axypet®
Pipettors
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual

Table of Contents
1. Product Description ..............................................................1
2. Setting the Volume................................................................5
3. Aspiration and Dispensing Instructions........................... 8
4. Pre-rinsing................................................................................ 9
5. Dense and Viscous Liquids.................................................10
6. Axygen® Pipet Tips...............................................................10
7. Recommendations...............................................................10
8. Recalibration .........................................................................11
9. Troubleshooting ...................................................................15
10. Cleaning and Sterilization..................................................17
11. Components..........................................................................18
12. Spare Parts .............................................................................18
13. Limited Warranty .................................................................19

Instruction Manual | 1
1. Product Description
The Axygen® Axypet® single-channel pipettor is a
volumetric instrument designed to measure and transfer
liquids precisely and safely. It can accommodate volumes
from 0.1 μL to 10,000 μL depending on the model.
Axygen Axypet 8-channel and 12-channel pipettors have
been designed for microplate filling. The pipettors enable
precise and simultaneous delivery of 8 or 12 preset volume
doses of liquid. These pipettors are produced in four volume
ranges: 0.5 to 10 μL, 5 to 50 μL, 20 to 200 μL, 50 to 300 μL.
Model Volume Range (μL) Application
2, 10
Measurement and transfer of
micro-volumes, DNA sequencing,
and enzyme assay applications.
20, 50, 100, 200,
250, 300, 1,000
Measurement and transfer of general
aqueous solution, acids, and bases.
5,000,
10,000
Measurement and transfer of large
volumes.
The single-, 8-, and 12-channel pipettors feature a digital
counter which shows the pipetting volume. The set
volume is visible in the window on the handle. Volumes
are set by either turning the pipetting pushbutton (A1) or
the adjustment knob (B) in the correct direction.
A1. Pushbutton
A2. Knob
B. Adjustment knob
P. Identication ring
C. Shaft
D. Ejector
E. Tip

2 | AXYGEN AXYPET PIPETTORS
The pipettor design allows the user to lock the volume
setting by sliding the locking ring upwards.
The symbols printed on the handle show locked and
unlocked positions. The volume range of each pipettor
model is shown on the pipetting pushbutton (A1).
Liquids are aspirated into disposable pipet tips attached
to the pipettor shaft. Disposable pipet tips ensure
maximum safety and eliminate the possibility of cross-
contamination of the liquid samples. To protect the user
from contaminated pipet tips, the pipettor is fitted with a
built-in tip ejector (D).
Single-channel Pipettor Ejector
The ejector length can be adjusted to accommodate all
common brands of pipet tips. When using narrow tubes,
it may be necessary to remove the tip ejector. This is done
simply by pulling down.
Adjusting the Tip Ejector Length
2 to 1,000 μL Pipettors
The “H“ spacers included with
each pipettor, allow the ejector
length to be adjusted by +1 mm
or +2 mm.
An ”H0” spacer is fitted as
standard. The outside shape
of the spacer identifies the
adjustment.
SS

Instruction Manual | 3
5,000 and 10,000 μL Pipettors
The length of tip ejector is adjusted by screwing in or
screwing out the tip ejector arbor with a small screwdriver.
Turn the screwdriver counterclockwise to increase the length
of the tip ejector, and clockwise to reduce the length of the
tip ejector. The ejector can be adjusted by up to 5 mm.
If the ejector adjustment described above is not sufficient
or the diameter of the ejector opening is too large to eject
the tip, it will be necessary to put the ejector cap “M” onto
the ejector.
2 and 10 μL Pipettors
The 2 μL to 10 μL pipettors may require the use of an
ejector cap “M” to efficiently eject certain brands of pipet
tips. Simply place the cap (supplied with the pipettor)
on the bottom of the pipettor shaft and slide the cap
upwards until it surrounds the bottom of the tip ejector.
L
MM
5,000 μL: L + 6 mm
10,000 μL: L + 10 mm

4 | AXYGEN AXYPET PIPETTORS
8-channel and 12-channel Pipettor Ejector
The 8-channel and 12-channel pipettor construction
allows the lower 8-channel and 12-channel module to be
rotated 360°. This allows the user to disable the ejector to
avoid accidental use and to change its position to make
filling of microplates more comfortable and convenient. It
is recommended to turn the module clockwise.
The pipettor is a high quality instrument which offers
excellent accuracy and precision.
The accuracy and precision (repeatability) of the liquid
volume depends on the quality of pipet tips used. The
values for accuracy and precision shown in the table below
were obtained using Axygen® pipet tips.
Model
Volume
Range
(μL) Cat. No.
Volume
(μL)
Accuracy
(%)
Precision
(%)
Fit to Tips
Without
Filter
(μL)
Single-channel Pipettors
2 AP-2
Min. 0.1 ±40.0 ≤12.0
101 ±2.7 ≤1.30
Max. 2 ±1.5 ≤0.70
10 AP-10
Min. 0.5 ±4.0 ≤2.80
105 ±1.0 ≤0.60
Max. 10 ±0.5 ≤0.40
20 AP-20
Min. 2 ±3.0 ≤1.50
20010 ±1.0 ≤0.50
Max. 20 ±0.8 ≤0.30
50 AP-50
Min. 5 ±2.5 ≤2.00
200
25 ±1.0 ≤0.60
Max. 50 ±0.8 ≤0.40
100 AP-100
Min. 10 ±1.6 ≤0.80
20050 ±0.8 ≤0.24
Max. 100 ±0.8 ≤0.20
200 AP-200
Min. 20 ±1.2 ≤0.60
200100 ±0.8 ≤0.25
Max. 200 ±0.6 ≤0.20
250 AP-250
Min. 50 ±1.0 ≤0.40
300125 ±0.8 ≤0.30
Max. 250 ±0.6 ≤0.30
1,000 AP-1000
Min. 100 ±1.6 ≤0.40
1,000500 ±0.7 ≤0.20
Max. 1,000 ±0.6 ≤0.15

Instruction Manual | 5
Model
Volume
Range
(μL) Cat. No.
Volume
(μL)
Accuracy
(%)
Precision
(%)
Fit to Tips
Without
Filter
(μL)
Single-channel Pipettors (continued)
5,000 AP-5000
Min. 1,000 ±0.6 ≤0.25
5,0002,500 ±0.6 ≤0.20
Max. 5,000 ±0.5 ≤0.15
10,000 AP-10ML
Min. 1,000 ±2.5 ≤0.60
10,0005,000 ±0.8 ≤0.30
Max. 10,000 ±0.5 ≤0.20
8-channel and 12-channel Pipettors
8 - 10
12 - 10
AP-8-10
AP-12-10
Min. 0.5 ±10.0 ≤8.0
105 ±4.0 ≤2.0
Max. 10 ±2.0 ≤1.2
8 - 50
12 - 50
AP-8-50
AP-12-50
Min. 5 ±4.0 ≤2.5
20025 ±3.0 ≤1.2
Max. 50 ±1.6 ≤0.6
8 - 200
12 - 200
AP-8-200
AP-12-200
Min. 20 ±3.0 ≤1.5
200100 ±1.5 ≤0.8
Max. 200 ±1.0 ≤0.6
8 - 300
12 - 300
AP-8-300
AP-12-300
Min. 50 ±1.6 ≤1.5
300150 ±1.2 ≤1.0
Max. 300 ±1.0 ≤0.6
The accuracy and precision are obtained gravimetrically using
Axygen® pipet tips, performing at least 10 measurements
of distilled water at a reference temperature of 20°C ± 1°C
according to EN ISO 8655 standard.
2. Setting the Volume
The volume of the pipettor is set by turning the pipetting
pushbutton (A2) or the black adjustment knob (B).
The volume display consists of three numbers and is
read from top to bottom. The three numbers indicate the
volume selected and are colored black and red.
Red numbers represent decimal fractions for microvolume
pipettors or milliliters for macrovolume pipettors.

6 | AXYGEN AXYPET PIPETTORS
An example for each of these pipettors is given below.
Model Volume
Range (μL)
Counter
Readings
Set
Volume Increment
Single-channel Pipettors
2
1
2
5
1.25 μL 0.002 μL
10
0
7
5
7.5 μL 0.02 μL
20
1
2
5
12.5 μL 0.02 μL
50
0
3
8
38.0 μL 0.2 μL
100
0
7
5
75.0 μL 0.2 μL
200
1
2
5
125.0 μL 0.2 μL
250
2
4
5
245.0 μL 0.2 μL
1,000
0
7
5
750.0 μL 2 μL
5,000
1
2
5
1.25 mL 10 μL
10,000
0
7
5
7.5 mL 20 μL

Instruction Manual | 7
Model Volume
Range (μL)
Counter
Readings
Set
Volume Increment
8-channel and 12-channel Pipettors
8 - 10
12 - 10
0
3
5
3.5 μL 0.02 μL
8 - 50
12 - 50
0
6
5
6.5 μL 0.10 μL
8 - 200
12 - 200
0
8
5
85.0 μL 0.20 μL
8 - 300
12 - 300
2
5
0
250.0 μL 1.0 μL
To attain the maximum accuracy, the set volume must be
approached from a higher value.
If the desired value is lower than the previous value, adjust
the value down to a one-third turn above the required
setting. If the desired value is higher than the previous
value, adjust the value to a one-third turn above the
required value. The setting should then be performed in
the following manner:
• Slowly turn the pipetting pushbutton or the black
adjustment knob to the required volume.
• If the knob is accidentally turned too far below the
required value, the process must be repeated.
• Set the locking ring into the upper position following
the volume adjustment, thus locking the knob and
preventing accidental volume change.

8 | AXYGEN AXYPET PIPETTORS
3. Aspiration and Dispensing Instructions
Single-channel Pipettors
Place a pipet tip on the shaft of the pipettor. See Section 6
for the appropriate tip. Press the pipet tip on firmly using a
slight twisting motion. This will ensure an airtight seal.
8-channel and 12-channel Pipettors
To put pipet tips on the shafts: hold the pipettor vertically
and press it against the tips in the rack box until the shafts
retract approximately 1.5 mm into the manifold. The
suspension system of the shafts ensures even and tight
sealing of the pipet tips. The rocking movement does not
have to be performed to seal the pipet tips tightly.
NOTE: Never handle a liquid with a pipettor which has not
been fitted with a pipet tip.
Aspiration
1. Press the pushbutton to the first positive stop. Holding
the pipettor vertically, immerse the pipet tip into the
sample liquid. The depth to which the pipet tip is
immersed in the sample liquid depends on the model.
Model Volume Range (μL) Immersion Depth (mm)
2, 10 ≤1
20, 50, 100 2 - 3
200, 250, 300, 1,000 2 - 4
5,000 3 - 6
10,000 5 - 7
1234
56

Instruction Manual | 9
2. Release the pipetting pushbutton slowly and smoothly
to aspirate the sample. Wait one second and then
withdraw the pipet tip from the liquid. If the pipet tip
is not immersed to the recommended depth or if the
pipetting pushbutton is rapidly released, air may enter
the disposable pipet tip.
NOTE: Avoid touching the orifice of the pipet tip.
Dispensing
3. Place the end of the pipet tip against the inside wall
of the vessel at an angle of 10° to 40°. Press the
pushbutton smoothly to the first stop. Wait one second.
4. Press the pushbutton to the second stop to expel any
remaining liquid. Keep the pushbutton depressed to the
very end, and remove the pipettor by drawing the pipet
tip against the inside surface of the receiving vessel.
5. Release the pushbutton to its starting position.
6. Eject the pipet tip by pressing the tip ejector button.
Remember to change the pipet tip whenever a different
type of liquid is to be sampled.
Filters
A replaceable filter (L), fitted in a seat in the bottom part
of the shaft, is used in 5,000 μL and 10,000 μL pipettors.
This filter prevents the aspirated liquid from entering
into the shaft and thus from contaminating the inside of
the shaft and the piston. Using the filter is particularly
important when aspirating and dispensing large volumes
of liquid.
If the filter becomes wet during liquid aspiration it should
be replaced with a new one.
4. Pre-rinsing
When pipetting liquids of higher viscosity or lower surface
tension than water (e.g., sera or organic solvents), a film
of liquid is formed on the inside wall of the pipet tip. This
film can create an error. As the film remains relatively
constant in successive pipetting operations with the same
tip, this error can be eliminated by forming the film before
transferring the first sample. This is done by aspirating a
sample and dispensing it back into the same vessel. Now,
as the film is already formed, all of the following samples
will have better accuracy and repeatability.
This pre-rinsing operation should be repeated when the
volume to be aspirated is changed or when a new pipet tip
is used.

10 | AXYGEN AXYPET PIPETTORS
5. Dense and Viscous Liquids
The pipettor’s specifications of accuracy and precision are
based on pipetting distilled water. Handling of liquids with
physical qualities of density, viscosity, and surface tension
which differ substantially from water may need to be
gravimetrically checked for compensation of the volume
setting. Normally the degree of error resulting from heavy
or viscous liquids is negligible if pipetting is done slowly
and carefully. It is most important to give the liquids
some time to react to the change of pressure by holding
the pipet tip in its position for at least 2 seconds after the
aspiration and the blow out stroke.
If in extreme cases, this method of operation does not
result in accurate values, compensation may be achieved
as follows:
• Weigh the pipetted liquid when the pipettor is set to
the nominal value. Then calculate the set-off from the
nominal value:
Correction, value = 2 x nominal val. —
m = weight of the sample
γ= density of liquid
• Check this operation once again and correct if necessary.
Note the corrected value for further pipetting of the
same kind of liquid.
6. Axygen® Pipet Tips
Axygen pipet tips are made from high performance
polypropylene and their quality ensures the precision
and accuracy associated with the pipettor. Strict control
is maintained throughout the manufacturing process to
ensure the highest quality.
The accuracy and precision figures for the pipettors are
only guaranteed when Axygen pipet tips are used. The
use of inferior quality tips will seriously degrade the
performance of the pipettor.
Refer to Pages 4 and 5 for information on proper pipet tips
sizes, accuracy, and precision values.
7. Recommendations
Observing the following recommendations will ensure
maximum possible accuracy and precision of liquid sampling.
• Make sure to operate the pipettor slowly and smoothly.
• The depth of immersion in the sample liquid should be
the minimum necessary and should remain constant
during aspiration.
γ
m

Instruction Manual | 11
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed or
when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end of
the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid
to be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipettor upside down.
- Never lay the pipettor on its side when there is liquid
in the tip.
• Never force the volume setting beyond its
recommended limits.
• When pipetting liquids with temperatures different
from the ambient temperature, it is recommended to
pre-rinse the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
finishing the pipetting operation.
8. Recalibration
The accuracy and precision of the pipettors are obtained
gravimetrically using Axygen® pipet tips, performing at
least 10 measurements of distilled water according to
EN ISO 8655 standard.
If during pipettor operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in Section 1, the pipettor recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been met
during error determination:
• Ambient temperature and the temperature of the
pipettor, pipet tips, and water were identical.
• Density of the liquid used is close to that of distilled water.
• Balance with appropriate sensitivity has been used.
• Conversion factor mg/μL has been taken into account.
• The requirements shown in Sections 3 and 7 have
been met.

12 | AXYGEN AXYPET PIPETTORS
Volume Checked
(μL)
Balance Sensitivity
(mg)
0.1 - 10 ≤0.001
10 - 100 ≤0.01
>100 ≤0.1
If the above conditions are satisfied and the accuracy
error for selected volume given in Section 1 exceeds the
permissible value, the pipettor recalibration procedure
should be continued.
The recalibration can be performed within one full turn of
the calibration key to the right or to the left only.
Recalibration Conditions
Ambient temperature and the temperature of the pipettor,
pipet tips, and liquid should be within the range of 20°C to
25°C and stabilized during weighing within ±0.5°C.
• Measurements should be conducted using distilled
water.
• Balance sensitivity should be suitable for the volume to
be measured.
Recalibration Procedure
• Set the dose volume depending on the pipettor volume
according to the following tables:
Model
Volume
Range
(μL)
Range of
Pipettor
Volumes (μL)
Preset
Volume
(μL)
Permissible
Volumes
(μL)
Volume Change
ΔV for Full Turn of
the Calibration Key
(24 Increments)
(μL)
Single-channel Pipettors
2 0.1 - 2 0.2 0.176 -
0.224
0.06
10 0.5 - 10 0.5 0.48 - 0.52 0.33
20 2 - 20 2 1.94 - 2.06 0.63
50 5 - 50 5 4.875 -
5.125
2.50
100 10 - 100 10 9.84 - 10.16 2.50
200 20 - 200 20 19.76 -
20.24
6.30
250 50 - 250 50 49.5 - 50.5 6.30
1,000 100 - 1,000 100 98.4 - 101.6 25.00
5,000 1,000 - 5,000 1,000 994 - 1,006 125.00

Instruction Manual | 13
Model
Volume
Range
(μL)
Range of
Pipettor
Volumes (μL)
Preset
Volume
(μL)
Permissible
Volumes
(μL)
Volume Change
ΔV for Full Turn of
the Calibration Key
(24 Increments)
(μL)
10,000 1,000 -
10,000
1,000 975 - 1,025 250.00
8-channel and 12-channel Pipettors
8 - 10
12 - 10 0.5 - 10 1 0.92 - 1.08 0.33
8 - 50
12 - 50 5 - 50 5 4.8 - 5.2 1.67
8 - 200
12 -
200
20 - 200 20 19.4 - 20.6 6.30
8 - 300
12 -
300
50 - 300 50 49.2 - 50.8 10.00
• Perform 5 aspirations, weigh each one, and calculate the
average value of the aspirations.
• Perform 3 aspiration series (each series should include
the aspirations from all channels), weigh each time, and
calculate the average value of the aspirations.
• Calculate average aspirated volume in μL multiplying
the average aspiration amount [mg] by the distilled
water density coefficient [μL/mg], which depends on
temperature and pressure according to the following
table.
Temperature
(°C)
Pressure (kPa)
95.0 101.3 105.0
20 1.0028 1.0029 1.0029
21 1.0030 1.0031 1.0031
22 1.0032 1.0033 1.0033
23 1.0034 1.0035 1.0036
24 1.0037 1.0038 1.0038

14 | AXYGEN AXYPET PIPETTORS
Temperature
(°C)
Pressure (kPa)
95.0 101.3 105.0
25 1.0039 1.0040 1.0040
If the average aspirated volume exceeds the permissible
value, the following should be performed:
12
34
K
1. Remove the pipetting pushbutton.
WARNING: The pipetting pushbutton consists of 2 parts:
the lower volume-setting section (A2) and the upper
button (A1). After removal of the pushbutton, both parts
will separate.
2. Holding the volume setting knob to protect it against
rotation, insert the calibration key into the cuts of the
calibration screw.
3. Turn the calibration key clockwise to reduce the aspirated
volume, or counterclockwise to increase the volume.
One full turn of the calibration key changes the pipettor
aspiration volume by the amount given in the table.
4. Take out the calibration key and fix the pipetting
pushbutton. The pipetting pushbutton should be fixed
by placing the lower section on the arbor first (A2) and
then the button (A1).
Determine the average aspirated volume. The average

Instruction Manual | 15
volume should be within the permissible range shown
in the table. If the volume exceeds the values stated, the
recalibration procedure should be repeated.
When pipetting liquids with physical properties that are
considerably different from those of water, follow the
rules as described in Section 5.
9. Troubleshooting
If you notice an improper pipettor operation, identify the
cause and eliminate the fault. Follow the instructions in
the sequence provided. Replacement of parts should be
required only occasionally and should not occur under
normal pipettor use.
Problem Cause Solution
Droplets of
liquid remain
in the pipet
tip.
The pipet tip is
emptied too fast.
Decrease the speed
of pressing the pipettor
pushbutton.
The pipet tip
wettability has
increased due to
extensive use.
Replace the tip with
a new one.
Droplets
of air appear
in the liquid
aspirated
into the
pipet tip.
The pipet tip
immersion
is too shallow.
Immerse the tip
deeper according to the
instructions.
The pipet tip is
incorrectly pressed
onto the pipettor
shaft.
Press the pipettor firmly.
The tip is damaged
or worn out due to
extensive use.
Replace the tip with
a new one.

16 | AXYGEN AXYPET PIPETTORS
Problem Cause Solution
The pipettor
incorrectly
aspirates
the liquid
or the liquid
drops out
from the tip.
The pipet tip is
incorrectly pressed
onto the pipettor
shaft.
Press the pipet tip firmly.
The shaft nut is
loose in the single-
channel pipettors
2 μL to 1,000 μL.
Tighten the shaft nut.
The sealing surface
of the shaft is
cracked or scored.
Remove the tip ejector.
Unscrew the shaft nut and
inspect the shaft and the
piston assembly. Replace
the damaged parts. When
reassembling the pipettor, the
nut should be hand-tightened.
In the single-channel pipettor
model, the damage of the
shaft may also cause a damage
of the piston assembly. Replace
the damaged parts. When
reassembling the pipettor, the
nut should be hand-tightened.
To remove the tip ejector in
models AP-5000 and AP-10ML,
remove the ejector pushbutton
and using a screwdriver
unscrew the tip ejector by
turning the screwdriver
counterclockwise.

Instruction Manual | 17
Problem Cause Solution
The pipettor
incorrectly
aspirates
the liquid or
liquid drops
out from the
pipet tip.
Damage to the
piston or seal due
to prolonged use
with chemically
aggressive liquids.
Replace the piston, seal,
and O-ring. Rinse the inside
of the shaft in distilled
water and dry. Lubricate the
seal and O-ring with the
lubricant which has been
included with each pipettor.
The replacement of the
piston requires conducting
the calibration procedure.
NOTE: The parts of the
single-channel (2 μL and
10 μL) pipettors should
be lubricated evenly with
a minimum amount of
lubricant.
The pipettor is
reassembled
improperly.
Disassemble the pipettor
and reassemble it, observing
the proper sequence of
steps.
No lubricant on the
sealing elements.
Remove the tip ejector.
Unscrew the shaft nut and
remove the shaft, piston
assembly, seal, and O-ring.
Rinse the removed parts
in distilled water and dry
thoroughly. Lightly lubricate
the inside surfaces of the
seal and the O-ring with
the included lubricant.
Reassemble the pipettor in
the reverse order.
Contamination of
the inside of the
pipettor caused by
extensive aspiration
of chemically
aggressive liquids or
because liquid got
inside the pipettor.
Contamination of the inside
of the pipettor caused
by extensive aspiration
of chemically aggressive
liquids or because liquid got
inside the pipettor.
If the problem continues after carrying out the above
steps, contact your Corning representative.
Before returning the pipettor, please ensure the pipettor
is completely free of any chemical, radioactive, or
microbiological contamination which could pose a threat
during transport and repair.

18 | AXYGEN AXYPET PIPETTORS
10. Cleaning and Sterilization
Cleaning
External surfaces of the pipetting pushbutton, the
ejector pushbutton, the handgrip, the shaft nut, and the
adjustment knob may be cleaned using a cloth dampened
in isopropyl alcohol. The remaining parts removed from
the pipettor during pipettor disassembly may be washed
with distilled water or isopropyl alcohol.
WARNING: Before using cleaning agents other than
those recommended by the manufacturer, check the
compatibility charts and consider chemical resistance of
the following plastics: PEI, PP, PC, POM, PA, PPS, PVDF used
to produce the pipettor parts.
Sterilization
The pipettor can be sterilized in an autoclave at 121°C
for 20 minutes. After sterilization, the pipettor should be
dried and cooled to room temperature. It is recommended
to:
• Unscrew the shaft nut slightly prior to sterilization and
re-tighten after autoclaving.
• Set the locking ring in lower (unlocked) position prior to
sterilization.
• Sterilize the pipettors in autoclave with an initial
vacuum and drying cycle.
• Prior to sterilization, unscrew the shaft nut slightly in
the AP-2 to AP-1000 pipettors, and unscrew the shaft
slightly in the AP-5000 and AP-10ML. After autoclaving,
these parts should be screwed tight again.
The precision of the results should not alter if the
pipetting process and autoclaving are carried out as
described in this manual. Because a slight change in the
accuracy of the dosage may occur, it is recommended to:
• Check the calibration of the pipettor after the initial
first, third, and fifth autoclaving cycles and then after
every 10 autoclaving cycles.
Ultra Violet (UV) Sterilization
The pipettors are UV-resistant. However, the
recommended distance from the radiation source to the
exposed element should be not less than 50 cm. Prolonged
and very intense UV exposure can cause decoloration of
pipettor parts without effecting their performance.